CN112410398A - Sterility detection method for crosslinked sodium hyaluronate gel for injection - Google Patents
Sterility detection method for crosslinked sodium hyaluronate gel for injection Download PDFInfo
- Publication number
- CN112410398A CN112410398A CN202011418806.4A CN202011418806A CN112410398A CN 112410398 A CN112410398 A CN 112410398A CN 202011418806 A CN202011418806 A CN 202011418806A CN 112410398 A CN112410398 A CN 112410398A
- Authority
- CN
- China
- Prior art keywords
- medium
- sodium hyaluronate
- test
- bacteria
- gel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/22—Testing for sterility conditions
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Epidemiology (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Public Health (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention provides a method for aseptically detecting cross-linked sodium hyaluronate gel for injection, which comprises the following steps: a1: preparing a bacterial suspension or a spore suspension; a2: preparation of a test solution: taking 90ml of hyaluronidase solution containing 300IU/ml, taking 10ml of crosslinked sodium hyaluronate gel for injection, putting the crosslinked sodium hyaluronate gel into the hyaluronidase solution, hydrolyzing at normal temperature, continuously shaking in the hydrolysis process, and shaking up after the gel is completely hydrolyzed to obtain the hyaluronic acid gel. By adopting the sterile detection method of the cross-linked sodium hyaluronate gel for injection, provided by the invention, hyaluronidase is adopted to dissolve a sample to be tested, so that the problem of false negative is avoided, direct inoculation is carried out, and microorganisms cannot be well detected due to the fact that the gel cannot be dissolved.
Description
Technical Field
The invention relates to the field of microbial detection, in particular to a sterile detection method of cross-linked sodium hyaluronate gel for injection.
Background
In the method for detecting the sterility of the cross-linked sodium hyaluronate gel for injection in the prior art, a sample is directly inoculated, and the gel cannot be dissolved, so that microorganisms cannot be well detected, and false negative results are easily caused.
Disclosure of Invention
The invention aims to provide a method for aseptically detecting cross-linked sodium hyaluronate gel for injection, which has high accuracy and is easy to realize.
The invention provides a method for aseptically detecting cross-linked sodium hyaluronate gel for injection, which comprises the following steps:
a1: preparing a bacterial suspension or a spore suspension;
a2: preparation of a test solution: taking 90ml of hyaluronidase solution containing 300IU/ml, taking 10ml of crosslinked sodium hyaluronate gel for injection, putting the crosslinked sodium hyaluronate gel into the hyaluronidase solution, hydrolyzing at normal temperature, continuously shaking in the hydrolysis process, and shaking uniformly after the gel is completely hydrolyzed to obtain the hyaluronic acid gel;
a3: the detection method comprises the following steps:
a 1: preparation of test article groups: filtering 200ml of sample solution by using a dual disposable totally-enclosed bacteria collection culture device, adding 100ml of thioglycollate fluid medium (FTM) culture medium into one bacteria collection cup of the bacteria collection culture device after the completion, and adding 100ml of trypticase soy peptone liquid medium (TSB) culture medium into the other bacteria collection cup;
a 2: preparation of test article test group: performing the same operation with the test sample group, preparing 3 bacteria collection cups for each culture medium of a thioglycollate fluid medium (FTM) and a trypticase soy peptone liquid medium (TSB), respectively, totally 6 bacteria collection cups, transferring to a positive control detection room after completion, and respectively adding test bacteria less than l00 cfu; wherein, bacterial suspension or spore suspension of staphylococcus aureus, escherichia coli and clostridium sporogenes is added into a thioglycollate fluid medium (FTM) culture medium, and bacterial suspension or spore suspension of candida albicans, aspergillus niger and bacillus subtilis is added into a trypticase soy peptone liquid medium (TSB) culture medium;
a 3: preparation of positive control group: taking 3 cups of disposable sterile bacteria collection cups containing 100ml of thioglycollate fluid medium (FTM) and 100ml of trypticase soy peptone liquid medium (TSB) medium respectively, and adding test bacteria with the same amount as the bacteria added in the test group of the test sample to a positive control detection room; wherein, bacterial suspension or spore suspension of staphylococcus aureus, escherichia coli and clostridium sporogenes is added into a thioglycollate fluid medium (FTM) culture medium, and bacterial suspension or spore suspension of candida albicans, aspergillus niger and bacillus subtilis is added into a trypticase soy peptone liquid medium (TSB) culture medium;
a 4: preparation of negative control group: filtering 200ml of hyaluronidase solution containing 300IU/ml by using a dual disposable totally-enclosed bacteria collection culture device, adding 100ml of thioglycollate fluid medium (FTM) culture medium into one bacteria collection cup of the bacteria collection culture device after the filtration is finished, and adding 100ml of trypticase soy peptone liquid medium (TSB) culture medium into the other bacteria collection cup;
a 5: colony count confirmation group: test bacteria with the same amount as that added in the test group added with the test sample are taken and added into corresponding culture media, staphylococcus aureus, bacillus subtilis and escherichia coli are subjected to colony number confirmation by using a trypticase soytone agar medium (TSA), candida albicans and aspergillus niger are subjected to colony number confirmation by using a Sabouraud glucose agar medium (SDA), and clostridium sporogenes are subjected to colony number confirmation by using a thioglycollate fluid medium (FTM).
a 6: and (5) culturing.
Further, in step A1, Staphylococcus aureus, Escherichia coli, Clostridium sporogenes, Bacillus subtilis, Candida albicans, and Aspergillus niger are made into bacterial suspension or spore suspension less than 100 cfu/ml.
Further, the preparation method of the hyaluronidase solution comprises the following steps: weighing a proper amount of hyaluronidase, dissolving in 100ml of 0.9% sterile sodium chloride solution, shaking to dissolve, and filtering with a sterile filter membrane of 0.22 μm in a sterile isolator after dissolving. A
Further, in step a2, the cross-linked sodium hyaluronate gel for injection was extruded from the syringe.
By adopting the sterile detection method of the cross-linked sodium hyaluronate gel for injection, provided by the invention, hyaluronidase is adopted to dissolve a sample to be tested, so that the problem of false negative is avoided, direct inoculation is carried out, and microorganisms cannot be well detected due to the fact that the gel cannot be dissolved.
Detailed Description
In the description of the present invention, it is to be noted that those whose specific conditions are not specified in the examples are carried out according to the conventional conditions or the conditions recommended by the manufacturers. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
The strains used in the following examples are as follows:
| serial number | Name (R) | Strain numbering | Algebra | Preparation and use |
| 1 | Staphylococcus aureus | CMCC(B)-26003 | No more than 5 th generation | Prepared on the same day and used within 2 hours |
| 2 | Escherichia coli | CMCC(B)44102 | No more than 5 th generation | Prepared on the same day and used within 2 hours |
| 3 | Clostridium sporogenes | CMCC(B)64941 | No more than 5 th generation | Prepared on the same day and used within 2 hours |
| 4 | Bacillus subtilis | CMCC(B)-63501 | No more than 5 th generation | Prepared on the same day and used within 2 hours |
| 5 | Candida albicans | CMCC(F)-98001 | No more than 5 th generation | Prepared on the same day and used within 2 hours |
| 6 | Aspergillus niger | CMCC(F)98003 | No more than 5 th generation | Prepared on the same day and used within 2 hours |
The strain used for inoculation has good morphological characteristics and the number of passages cannot exceed 5.
The embodiment provides a method for detecting sterility of cross-linked sodium hyaluronate gel for injection, which comprises the following steps:
a1: preparing a bacterial suspension or a spore suspension;
a2: preparation of a test solution: taking 90ml of hyaluronidase solution containing 300IU/ml, taking 10ml of crosslinked sodium hyaluronate gel for injection, putting the crosslinked sodium hyaluronate gel into the hyaluronidase solution, hydrolyzing at normal temperature, continuously shaking in the hydrolysis process, and shaking uniformly after the gel is completely hydrolyzed to obtain the hyaluronic acid gel;
a3: the detection method comprises the following steps:
a 1: preparation of test article groups: filtering 200ml of sample solution by using a dual disposable totally-enclosed bacteria collection culture device, adding 100ml of thioglycollate fluid medium (FTM) culture medium into one bacteria collection cup of the bacteria collection culture device after the completion, and adding 100ml of trypticase soy peptone liquid medium (TSB) culture medium into the other bacteria collection cup;
a 2: preparation of test article test group: performing the same operation with the test sample group, preparing 3 bacteria collecting cups respectively for each culture medium of a thioglycollate fluid medium (FTM) and a trypticase soy peptone liquid medium (TSB), transferring to a positive control detection room after completing the preparation, and respectively adding test bacteria less than 100 cfu; wherein, bacterial suspension or spore suspension of staphylococcus aureus, escherichia coli and clostridium sporogenes is added into a thioglycollate fluid medium (FTM) culture medium, and bacterial suspension or spore suspension of candida albicans, aspergillus niger and bacillus subtilis is added into a trypticase soy peptone liquid medium (TSB) culture medium;
a 3: preparation of positive control group: taking 3 cups of disposable sterile bacteria collection cups containing 100ml of thioglycollate fluid medium (FTM) and 100ml of trypticase soy peptone liquid medium (TSB) medium respectively, and adding test bacteria with the same amount as the bacteria added in the test group of the test sample to a positive control detection room; wherein, bacterial suspension or spore suspension of staphylococcus aureus, escherichia coli and clostridium sporogenes is added into a thioglycollate fluid medium (FTM) culture medium, and bacterial suspension or spore suspension of candida albicans, aspergillus niger and bacillus subtilis is added into a trypticase soy peptone liquid medium (TSB) culture medium;
a 4: preparation of negative control group: filtering 200ml of hyaluronidase solution containing 300IU/ml by using a dual disposable totally-enclosed bacteria collection culture device, adding 100ml of thioglycollate fluid medium (FTM) culture medium into one bacteria collection cup of the bacteria collection culture device after the filtration is finished, and adding 100ml of trypticase soy peptone liquid medium (TSB) culture medium into the other bacteria collection cup;
a 5: colony count confirmation group: test bacteria with the same amount as that added in the test group added with the test sample are taken and added into corresponding culture media, staphylococcus aureus, bacillus subtilis and escherichia coli are subjected to colony number confirmation by using a trypticase soytone agar medium (TSA), candida albicans and aspergillus niger are subjected to colony number confirmation by using a Sabouraud glucose agar medium (SDA), and clostridium sporogenes are subjected to colony number confirmation by using a thioglycollate fluid medium (FTM).
a 6: culturing: : placing tryptone soy peptone liquid medium (TSB) of the test sample test group and the positive control group and Sa's dextrose agar medium (SDA) of the colony number confirmation group at 20-25 ℃ for culturing for 5 days, placing the thioglycolate fluid medium (FTM) of the test sample test group and the positive control group and the tryptone soy peptone agar medium (TSA) and the thioglycolate fluid medium (FTM) of the colony number confirmation group at 30-35 ℃ for culturing for 5 days, placing the tryptone soy peptone liquid medium (TSB) of the test sample group and the negative control group at 20-25 ℃ for culturing for 14 days, and placing the thioglycolate fluid medium (FTM) of the test sample group and the negative control group at 30-35 ℃ for culturing for 14 days.
In this example, the tryptic Soy peptone liquid Medium (TSB) of the test sample test group and the positive control group and the Sabourdon dextrose agar Medium (SDA) of the colony number confirmation group were incubated at 22 ℃ for 5 days, the thioglycolate fluid Medium (FTM) of the test sample test group and the positive control group and the tryptic Soy peptone agar Medium (TSA) and the thioglycolate fluid Medium (FTM) of the colony number confirmation group were incubated at 33 ℃ for 5 days, the tryptic Soy peptone liquid Medium (TSB) of the test sample group and the negative control group was incubated at 22 ℃ for 14 days, and the thioglycolate fluid Medium (FTM) of the test sample group and the negative control group was incubated at 33 ℃ for 14 days.
In step A1, Staphylococcus aureus, Escherichia coli, Clostridium sporogenes, Bacillus subtilis, Candida albicans, and Aspergillus niger are made into bacterial suspension or spore suspension less than 100 cfu/ml.
The preparation method of the hyaluronidase solution comprises the following steps: weighing a proper amount of hyaluronidase, dissolving in 100ml of 0.9% sterile sodium chloride solution, shaking to dissolve, and filtering with a sterile filter membrane of 0.22 μm in a sterile isolator after dissolving.
Wherein, in the step A2, the cross-linked sodium hyaluronate gel for injection is extruded from the needle tube.
As a result: the negative control group and the test sample group grow aseptically, the positive control group grows well, compared with the control group, the test bacteria of the test sample group grow well, the result shows that the test quantity of the test sample has no bacteriostasis or the bacteriostasis can be ignored under the test condition, the colony number of the colony number confirmation group meets the requirement and is not more than 100cfu/ml, and the sterility test of the test sample can be carried out according to the test method and the test condition.
By adopting the sterile detection method of the cross-linked sodium hyaluronate gel for injection provided by the embodiment of the invention, hyaluronidase is adopted to dissolve a sample to be tested, so that the problem of false negative is avoided, direct inoculation is carried out, and microorganisms cannot be well detected due to the fact that the gel cannot be dissolved.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (4)
1. The method for detecting the sterility of the crosslinked sodium hyaluronate gel for injection is characterized by comprising the following steps:
a1: preparing a bacterial suspension or a spore suspension;
a2: preparation of a test solution: taking 90ml of hyaluronidase solution containing 300IU/ml, taking 10ml of crosslinked sodium hyaluronate gel for injection, putting the crosslinked sodium hyaluronate gel into the hyaluronidase solution, hydrolyzing at normal temperature, continuously shaking in the hydrolysis process, and shaking uniformly after the gel is completely hydrolyzed to obtain the hyaluronic acid gel;
a3: the detection method comprises the following steps:
a 1: preparation of test article groups: filtering 200ml of sample solution by using a dual disposable totally-enclosed bacteria collection culture device, adding 100ml of thioglycollate fluid medium (FTM) culture medium into one bacteria collection cup of the bacteria collection culture device after the completion, and adding 100ml of trypticase soy peptone liquid medium (TSB) culture medium into the other bacteria collection cup;
a 2: preparation of test article test group: performing the same operation with the test sample group, preparing 3 bacteria collection cups for each culture medium of a thioglycollate fluid medium (FTM) and a trypticase soy peptone liquid medium (TSB), respectively, totally 6 bacteria collection cups, transferring to a positive control detection room after completion, and respectively adding test bacteria less than l00 cfu; wherein, bacterial suspension or spore suspension of staphylococcus aureus, escherichia coli and clostridium sporogenes is added into a thioglycollate fluid medium (FTM) culture medium, and bacterial suspension or spore suspension of candida albicans, aspergillus niger and bacillus subtilis is added into a trypticase soy peptone liquid medium (TSB) culture medium;
a 3: preparation of positive control group: taking 3 cups of disposable sterile bacteria collection cups containing 100ml of thioglycollate fluid medium (FTM) and 100ml of trypticase soy peptone liquid medium (TSB) medium respectively, and adding test bacteria with the same amount as the bacteria added in the test group of the test sample to a positive control detection room; wherein, bacterial suspension or spore suspension of staphylococcus aureus, escherichia coli and clostridium sporogenes is added into a thioglycollate fluid medium (FTM) culture medium, and bacterial suspension or spore suspension of candida albicans, aspergillus niger and bacillus subtilis is added into a trypticase soy peptone liquid medium (TSB) culture medium;
a 4: preparation of negative control group: filtering 200ml of hyaluronidase solution containing 300IU/ml by using a dual disposable totally-enclosed bacteria collection culture device, adding 100ml of thioglycollate fluid medium (FTM) culture medium into one bacteria collection cup of the bacteria collection culture device after the filtration is finished, and adding 100ml of trypticase soy peptone liquid medium (TSB) culture medium into the other bacteria collection cup;
a 5: colony count confirmation group: test bacteria with the same amount as that added in the test group added with the test sample are taken and added into corresponding culture media, staphylococcus aureus, bacillus subtilis and escherichia coli are subjected to colony number confirmation by using a trypticase soytone agar medium (TSA), candida albicans and aspergillus niger are subjected to colony number confirmation by using a Sabouraud glucose agar medium (SDA), and clostridium sporogenes are subjected to colony number confirmation by using a thioglycollate fluid medium (FTM).
a 6: and (5) culturing.
2. The method for aseptically detecting cross-linked sodium hyaluronate gel for injection according to claim 1, wherein in step A1, Staphylococcus aureus, Escherichia coli, Clostridium sporogenes, Bacillus subtilis, Candida albicans and Aspergillus niger are made into bacterial suspension or spore suspension of less than 100 cfu/ml.
3. The method for aseptically detecting the crosslinked sodium hyaluronate gel for injection according to claim 1, wherein the preparation method of the hyaluronidase solution comprises the following steps: weighing a proper amount of hyaluronidase, dissolving in 100ml of 0.9% sterile sodium chloride solution, shaking to dissolve, and filtering with a sterile filter membrane of 0.22 μm in a sterile isolator after dissolving.
4. The method for aseptically measuring crosslinked sodium hyaluronate gel for injection according to claim 1, wherein in step A2, the crosslinked sodium hyaluronate gel for injection is extruded from a needle tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011418806.4A CN112410398A (en) | 2020-12-07 | 2020-12-07 | Sterility detection method for crosslinked sodium hyaluronate gel for injection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011418806.4A CN112410398A (en) | 2020-12-07 | 2020-12-07 | Sterility detection method for crosslinked sodium hyaluronate gel for injection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112410398A true CN112410398A (en) | 2021-02-26 |
Family
ID=74776741
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011418806.4A Pending CN112410398A (en) | 2020-12-07 | 2020-12-07 | Sterility detection method for crosslinked sodium hyaluronate gel for injection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112410398A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105572062A (en) * | 2015-12-24 | 2016-05-11 | 杭州嘉伟生物制品有限公司 | Method for determining in-vitro enzymatic hydrolysis rate of crosslinked sodium hyaluronate gel |
| CN106248819A (en) * | 2016-07-13 | 2016-12-21 | 浙江景嘉医疗科技有限公司 | The detection method of lidocaine hydrochloride content in a kind of medical cross-linking sodium hyaluronate gel |
| CN108300755A (en) * | 2018-01-26 | 2018-07-20 | 北京朗依制药有限公司 | The microbial limit tests of dispersible tablet of pidotimod |
| CN111500671A (en) * | 2020-04-13 | 2020-08-07 | 苏州市药品检验检测研究中心 | Method for checking sterility of docetaxel micelle for injection |
-
2020
- 2020-12-07 CN CN202011418806.4A patent/CN112410398A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105572062A (en) * | 2015-12-24 | 2016-05-11 | 杭州嘉伟生物制品有限公司 | Method for determining in-vitro enzymatic hydrolysis rate of crosslinked sodium hyaluronate gel |
| CN106248819A (en) * | 2016-07-13 | 2016-12-21 | 浙江景嘉医疗科技有限公司 | The detection method of lidocaine hydrochloride content in a kind of medical cross-linking sodium hyaluronate gel |
| CN108300755A (en) * | 2018-01-26 | 2018-07-20 | 北京朗依制药有限公司 | The microbial limit tests of dispersible tablet of pidotimod |
| CN111500671A (en) * | 2020-04-13 | 2020-08-07 | 苏州市药品检验检测研究中心 | Method for checking sterility of docetaxel micelle for injection |
Non-Patent Citations (4)
| Title |
|---|
| 国家药典委员会: "《中华人民共和国药典》", 30 June 2015, 中国医药科技出版社 * |
| 国家药典委员会: "《中国药典分析检测技术指南》", 30 July 2017, 中国医药科技出版社 * |
| 曲建全 等: "透明质酸钠的无菌检查", 《2003年全国生化与生物技术药物学术研讨会论文集》 * |
| 葛雪飞 等: "交联透明质酸钠凝胶中盐酸利多卡因的HPLC测定", 《广州化工》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Rupp et al. | Adequate disinfection of a split-septum needleless intravascular connector with a 5-second alcohol scrub | |
| Hassan et al. | Detection and antibiotic susceptibility pattern of biofilm producing Gram positive and Gram negative bacteria isolated from a tertiary care hospital of Pakistan | |
| CN109880758B (en) | Lactobacillus plantarum mutant strain and mutation method and application thereof | |
| CN114480555B (en) | Method for rapidly detecting sterility of sterile bulk drug | |
| Gomez-Garces et al. | Bacteriologic characteristics and antimicrobial susceptibility of 70 clinically significant isolates of Streptococcus milleri group | |
| CN111500671A (en) | Method for checking sterility of docetaxel micelle for injection | |
| CN112410398A (en) | Sterility detection method for crosslinked sodium hyaluronate gel for injection | |
| CN111850086B (en) | Aseptic detection method of voriconazole for injection | |
| CN110484596B (en) | Method for checking sterility of gentamicin sulfate injection | |
| CN105713955A (en) | Culture medium for identifying group-B streptococcus | |
| Martini et al. | Investigation of biofilm formation in coagulase-negative staphylococci isolated from platelet concentrate bags | |
| Dilip et al. | Speciation, Detection of Virulence Factors and Antibiotic Susceptibility of Coagulase Negative Staphylococci | |
| Schneierson et al. | Erwinia infections in man | |
| CN109402219B (en) | Sterility inspection method of azithromycin eye drops | |
| CN116024114A (en) | Intestinal bacillus subtilis strain and preparation method and application thereof | |
| CN110804646B (en) | Antibacterial activity removal method for rifampicin capsule microorganism limit control bacteria inspection | |
| CN113293194A (en) | Sterility test method for moxifloxacin hydrochloride eye drops | |
| CN114574331A (en) | Sterility test and verification method for cell product | |
| CN112831539A (en) | In-vitro detection kit for aerobic microorganisms | |
| KR20200079713A (en) | Agent simulant composition of gram positive bacterial and manufacturing method thereof | |
| RU2446214C1 (en) | Method of determining degree of epidemic hazard of pathogenic and potentially pathogenic bacteria isolated from water for various uses | |
| Gould et al. | Does storage of sputum specimens adversely affect culture results? | |
| CN111286524A (en) | Method for checking total number of aerobic bacteria of nifuratel tablets | |
| Denyer et al. | Introduction to pharmaceutical microbiology | |
| CN118272484A (en) | Microorganism limit inspection method for preparation containing boric acid antibacterial component |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210226 |