CN112391301A - Construction and culture method of propionibacterium acnes biofilm for experimental research - Google Patents
Construction and culture method of propionibacterium acnes biofilm for experimental research Download PDFInfo
- Publication number
- CN112391301A CN112391301A CN201910737263.3A CN201910737263A CN112391301A CN 112391301 A CN112391301 A CN 112391301A CN 201910737263 A CN201910737263 A CN 201910737263A CN 112391301 A CN112391301 A CN 112391301A
- Authority
- CN
- China
- Prior art keywords
- bhi
- culture
- propionibacterium acnes
- adjusting
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000186427 Cutibacterium acnes Species 0.000 title claims abstract description 37
- 229940055019 propionibacterium acne Drugs 0.000 title claims abstract description 37
- 238000010276 construction Methods 0.000 title abstract description 15
- 238000012136 culture method Methods 0.000 title abstract description 13
- 239000001963 growth medium Substances 0.000 claims abstract description 28
- 241000894006 Bacteria Species 0.000 claims abstract description 22
- 238000000034 method Methods 0.000 claims abstract description 20
- 238000012258 culturing Methods 0.000 claims abstract description 9
- 238000005303 weighing Methods 0.000 claims abstract description 6
- 239000002994 raw material Substances 0.000 claims abstract description 5
- 229940041514 candida albicans extract Drugs 0.000 claims description 16
- 239000012138 yeast extract Substances 0.000 claims description 16
- 229920001817 Agar Polymers 0.000 claims description 14
- 239000008272 agar Substances 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 12
- 239000008103 glucose Substances 0.000 claims description 12
- 239000000843 powder Substances 0.000 claims description 12
- 238000002156 mixing Methods 0.000 claims description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 6
- 241001052560 Thallis Species 0.000 claims description 6
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 239000002609 medium Substances 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- 239000008280 blood Substances 0.000 claims description 4
- 210000004369 blood Anatomy 0.000 claims description 4
- 239000006781 columbia blood agar Substances 0.000 claims description 4
- 238000011084 recovery Methods 0.000 claims description 4
- 208000002874 Acne Vulgaris Diseases 0.000 claims description 3
- 206010000496 acne Diseases 0.000 claims description 3
- 230000010355 oscillation Effects 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 238000010257 thawing Methods 0.000 claims description 2
- 239000012499 inoculation medium Substances 0.000 claims 1
- 238000002955 isolation Methods 0.000 claims 1
- 230000003902 lesion Effects 0.000 claims 1
- 241000894007 species Species 0.000 claims 1
- 238000000338 in vitro Methods 0.000 abstract description 5
- 239000000243 solution Substances 0.000 description 9
- 238000004113 cell culture Methods 0.000 description 8
- 239000003242 anti bacterial agent Substances 0.000 description 4
- 229940088710 antibiotic agent Drugs 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 230000003115 biocidal effect Effects 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 206010059866 Drug resistance Diseases 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 230000009885 systemic effect Effects 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- OJMMVQQUTAEWLP-UHFFFAOYSA-N Lincomycin Natural products CN1CC(CCC)CC1C(=O)NC(C(C)O)C1C(O)C(O)C(O)C(SC)O1 OJMMVQQUTAEWLP-UHFFFAOYSA-N 0.000 description 1
- 102000007474 Multiprotein Complexes Human genes 0.000 description 1
- 108010085220 Multiprotein Complexes Proteins 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 238000010009 beating Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000032770 biofilm formation Effects 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 210000003780 hair follicle Anatomy 0.000 description 1
- 230000005745 host immune response Effects 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- OJMMVQQUTAEWLP-KIDUDLJLSA-N lincomycin Chemical compound CN1C[C@H](CCC)C[C@H]1C(=O)N[C@H]([C@@H](C)O)[C@@H]1[C@H](O)[C@H](O)[C@@H](O)[C@@H](SC)O1 OJMMVQQUTAEWLP-KIDUDLJLSA-N 0.000 description 1
- 229960005287 lincomycin Drugs 0.000 description 1
- 239000003120 macrolide antibiotic agent Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 244000039328 opportunistic pathogen Species 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 210000001732 sebaceous gland Anatomy 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 206010040882 skin lesion Diseases 0.000 description 1
- 231100000444 skin lesion Toxicity 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Cosmetics (AREA)
Abstract
The invention belongs to the technical field of biology, and relates to a construction and culture method of a propionibacterium acnes biofilm for experiments; the method comprises the following steps: weighing raw materials, and preparing a culture medium to obtain a BHI Sup. culture medium; recovering the seed bacteria; resuspending the thallus; and (5) culturing strains. According to the invention, the prepared BHI Sup culture medium is cultured in an anaerobic environment, so that the problems that the propionibacterium acnes needs a microaerophilic or anaerobic environment for growth, the growth rate is slow, the biomembrane is difficult to construct in vitro by adopting the prior art and the like can be effectively solved.
Description
Technical Field
The invention belongs to the technical field of biology, and relates to a construction and culture method of a propionibacterium acnes biofilm for experimental research.
Background
The prior art discloses that propionibacterium acnes is an anaerobic gram-positive bacillus which is mainly planted in a skin hair follicle sebaceous gland unit and is a symbiotic flora of human skin. Propionibacterium acnes play an important role in the development of acne and, in addition, can also serve as an opportunistic pathogen causing a variety of implant-related infections. Currently, systemic or topical antibiotics are widely used to treat infections caused by propionibacterium acnes, however, the resistance rate of propionibacterium acnes to antibiotics is increasing; according to research reports, the drug resistance rate of the compound to antibiotics is up to 55.5%, wherein the drug resistance rate to macrolide antibiotics and lincomycin antibiotics is highest. In recent years, there has been an increasing search for antibiotic resistance as a result of bacterial biofilm formation.
The bacterial biofilm is a survival mode corresponding to planktonic cells, which is formed by bacteria adsorbed on the surfaces of inert or active materials in the growth process for adapting to the living environment, and comprises three key components: bacterial cells, a plane for attachment of bacteria, and extracellular polysaccharide protein complexes; the latter are secreted by bacteria and can protect the bacteria from host immune responses and local and systemic antibiotic treatment. Because the propionibacterium acnes grows slowly and is clinically suspected to be serious or deep infection caused by the propionibacterium acnes, anaerobic culture needs to be prolonged to 14 days, and the environmental conditions required for growth are strict (microaerophilic or anaerobic), the difficulty in constructing the biofilm in vitro is high, and the biofilm which is difficult to form well according to the conventional biofilm construction scheme is high.
Based on the current situation of the prior art, the inventor of the application intends to provide a method for constructing and culturing a propionibacterium acnes biofilm for experimental research.
Disclosure of Invention
The invention aims to overcome the defects or shortcomings of the prior art and provide a method for constructing and culturing a propionibacterium acnes biomembrane for experimental research, and the method can effectively solve the problem that the prior art is not ideal for in-vitro construction of the propionibacterium acnes biomembrane.
The invention discloses a method for constructing and culturing a Propionibacterium acnes biomembrane for experimental research, which is characterized by comprising the following steps of:
step 1, weighing raw materials: BHI dry powder, yeast extract;
step 2, preparation of a culture medium: dissolving BHI dry powder and yeast extract in water, adding glucose to prepare 1% glucose solution, mixing and stirring uniformly to obtain BHI Sup culture medium, and adjusting pH value of the culture medium to 7.0;
step 3, seed bacteria recovery: thawing cryopreserved propionibacterium acnes strain at room temperature, streaking strain on a blood plate, inoculating the strain to Brookfield agar broth after the bacteria are recovered, adjusting OD600nm of the strain, and performing anaerobic culture by using a shaking incubator;
step 4, resuspending the thalli: collecting cultured thalli by using a sterile centrifuge tube, centrifuging, removing supernatant, using Brookfield agar broth to resuspend the thalli, and adjusting the OD600nm value to 1.0;
step 5, strain culture: the resuspended cells were diluted with BHI Sup. medium, added to 96-well plates, and cultured anaerobically.
In step 1 of the invention, 37g of BHI dry powder and 5g of yeast extract are weighed;
in step 2 of the invention: dissolving 37g of BHI dry powder and 5g of yeast extract in 1L of water, adding glucose to prepare a 1% glucose solution, uniformly stirring to obtain a BHI Sup culture medium, and adjusting the pH value of the culture medium to 7.0;
in step 3 of the invention: after the bacteria are recovered, inoculating the bacteria in Brookfield agar broth, adjusting the OD600nm of the strains to be 0.1, adjusting the oscillation speed to 220rpm, and carrying out anaerobic culture at 37 ℃ for 120 h;
in step 4 of the invention: treating the cultured thallus with a centrifuge at 6000rpm for 5min, and adjusting the OD600nm value of the resuspended thallus to 1.0;
in step 5 of the invention: the resuspended cells were diluted with BHI Sup. medium at a ratio of 1:100, added to a 96-well plate at 200 ul/well, and anaerobically cultured at 37 ℃ for 120 h.
In the construction and culture method, the strain of the cryopreserved propionibacterium acnes is taken from the skin lesion of an acne patient and is obtained after separation and identification, so that the purity of the propionibacterium acnes is ensured;
in the construction culture method, the obtained strain is placed in glycerol and stored at the temperature of minus 80 ℃;
in the construction culture method, the water is deionized water;
in the construction and culture method, the blood plate adopts a Columbia blood agar plate;
in the construction and culture method, the recovered strain is inoculated in Brookfield agar broth;
in the construction culture method, the culture medium used for resuspension is Brookfield agar broth;
in the step 2 and the step 5, the culture temperature is 37 ℃;
in the step 2 and the step 5, the culture environment is anaerobic environment;
in the step 5, the strain culture is carried out on a 96-hole cell culture plate;
in the construction culture method, the yeast extract adopts a high-purity high-protein yeast extract.
The invention has the beneficial effects that:
(1) the prepared BHI Sup culture medium is used for culturing the propionibacterium acnes in an anaerobic environment, so that the problems that the propionibacterium acnes grow under severe conditions (anaerobic or microaerophilic), the growth rate is slow, and the biomembrane is difficult to construct in vitro by adopting the prior art are solved.
(2) The BHI Sup culture medium is low in price and easy to prepare, the biomass of the Propionibacterium acnes biomembrane, the number of viable bacteria in the biomembrane and the structure of the biomembrane constructed by the BHI Sup culture medium are superior to those of the conventional culture medium, the BHI Sup culture medium is an ideal culture medium for researching the Propionibacterium acnes biomembrane, and a foundation is laid for the subsequent research on the Propionibacterium acnes biomembrane and the effect of the Propionibacterium acnes biomembrane in antibiotic resistance.
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Detailed Description
Example 1
A construction and culture method of a Propionibacterium acnes biomembrane for experimental research comprises the following steps;
step 1: weighing raw materials, namely weighing 30g of BHI dry powder, 5g of yeast extract, 1% glucose solution prepared from glucose and 1L of deionized water;
step 2: preparing a culture medium, mixing the yeast extract with deionized water, adding 37g of BHI dry powder, uniformly mixing and stirring to obtain a BHI Sup. culture medium, wherein the pH value of the culture medium is 7.0;
and step 3: recovering strain, melting cryopreserved propionibacterium acnes strain at room temperature, streaking strain on Columbia blood agar plate, inoculating bacteria to Brookfield agar broth after recovery, adjusting OD600nm of strain to 0.1, and performing anaerobic culture in a shaking incubator at 37 deg.C and 220rpm for 120 h;
and 4, step 4: resuspending thallus, collecting cultured thallus with sterile centrifuge tube, treating with centrifuge at 6000rpm for 5min, taking out supernatant, resuspending thallus with Buchner agar broth, and adjusting OD600nm to 1.0;
and 5: the strain is cultured, the resuspended thallus is diluted by BHI Sup. culture medium according to the proportion of 1:100, then 96-hole cell culture plates (200 ul/hole) are added, and the culture is carried out for 120h under the anaerobic environment at 37 ℃.
Example 2
The present example further provides a semi-quantitative detection method of a propionibacterium acnes biofilm, in the above example 1, comprising the following steps:
step 1: when propionibacterium acnes were cultured in vitro using 96-well cell culture plates for 96h and 120h, the following operations were performed, respectively: discarding bacteria solution, adding PBS to wash off non-adhered bacteria, and repeating washing for 3 times;
step 2: adding 99% methanol into 96-well cell culture plate washed with PBS to fix for 15min, removing liquid, and drying at room temperature;
and step 3: adding 1% crystal violet into a 96-hole cell culture plate, dyeing for 8min, washing with tap water until running water is colorless, and drying at room temperature;
and 4, step 4: the biofilm was dissolved by adding a 10% acetic acid solution to a 96-well cell culture plate, dried at room temperature, and then measured for OD570nm using a spectrophotometer.
Example 3
The present example further provides, in example 1 above, a method for detecting the number of viable bacteria in a propionibacterium acnes biofilm, including the following steps:
step 1: at 96h and 120h of culture using 96-well cell culture plates, the following operations were performed, respectively: discarding bacteria solution, adding PBS to wash off non-adhered bacteria, and repeating washing for 3 times;
step 2: adding 100ul of normal saline into each hole of a 96-hole cell culture plate, and repeatedly blowing and beating to completely resuspend bacteria in the biomembrane in the normal saline;
and 4, step 4: respectively transferring the bacterial liquid after the heavy suspension in each hole into a white 96-hole enzyme label plate, adding 20ul CellTiter-blue (TM) reagent into each hole, shaking for 1min at 300rpm by using a mixing instrument, culturing for 1h at 37 ℃ in an anaerobic environment, and recording the fluorescence of Em560/Ex590nm by using an enzyme label instrument;
example 4
A construction and culture method of a Propionibacterium acnes biomembrane for experimental research and an observation method of the morphological structure of the Propionibacterium acnes biomembrane are provided, and the method comprises the following steps:
step 1: weighing raw materials to obtain BHI dry powder 37g, yeast extract 5g and deionized water 1L;
step 2: preparing a culture medium, mixing the yeast extract, BHI dry powder and deionized water, adding glucose to prepare a 1% glucose solution, mixing and stirring uniformly to prepare a BHI Sup culture medium, and adjusting the pH value of the culture medium to 7.0;
and step 3: recovering strain, melting cryopreserved propionibacterium acnes strain at room temperature, streaking strain on Columbia blood agar plate, inoculating bacteria to Brookfield agar broth after recovery, adjusting OD600nm of strain to 0.1, and performing anaerobic culture in a shaking incubator at 37 deg.C and 220rpm for 120 h;
and 4, step 4: resuspending thallus, collecting cultured thallus with sterile centrifuge tube, treating with centrifuge at 6000rpm for 5min, discarding supernatant, resuspending thallus with Buchner agar broth, and adjusting OD600nm to 1.0;
and 5: the strain is cultured, diluted by BHI Sup. culture medium at a ratio of 1:10, and then fluorodish (2 ml/dish) is added to the strain, and the strain is cultured for 120 hours in an anaerobic environment at 37 ℃.
Step 6: the supernatant was discarded, gently washed with physiological saline for 3 times, added with 600ul Live/Dead dye solution, left at room temperature for 20min, and observed under a confocal laser microscope. .
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (11)
1. A method for constructing and culturing a Propionibacterium acnes biofilm for experimental research is characterized by comprising the following steps:
step 1, weighing raw materials: BHI dry powder, yeast extract;
step 2, preparation of a culture medium: dissolving BHI dry powder and yeast extract in water, adding glucose to prepare 1% glucose solution, mixing and stirring uniformly to obtain BHI Sup culture medium, and adjusting pH value of the culture medium to 7.0;
step 3, seed bacteria recovery: thawing cryopreserved propionibacterium acnes strain at room temperature, streaking strain on a blood plate, inoculating the strain to Brookfield agar broth after the bacteria are recovered, adjusting OD600nm of the strain, and performing anaerobic culture by using a shaking incubator;
step 4, resuspending the thalli: collecting cultured thalli by using a sterile centrifuge tube, centrifuging, removing supernatant, using Brookfield agar broth to resuspend the thalli, and adjusting the OD600nm value to 1.0;
step 5, strain culture: the resuspended cells were diluted with BHI Sup. medium, added to 96-well plates, and cultured anaerobically.
2. The method of claim 1, wherein in step 1, 37g of BHI dry powder, 5g of yeast extract;
in the step 2: dissolving 37g of BHI dry powder and 5g of yeast extract in 1L of water, adding glucose to prepare a 1% glucose solution, uniformly stirring to obtain a BHI Sup culture medium, and adjusting the pH value of the culture medium to 7.0;
in the step 3: after the bacteria are recovered, inoculating the bacteria in Brookfield agar broth, adjusting the OD600nm of the strains to be 0.1, adjusting the oscillation speed to 220rpm, and carrying out anaerobic culture at 37 ℃ for 120 h;
in the step 4: treating the cultured thallus with a centrifuge at 6000rpm for 5min, and adjusting the OD600nm value of the resuspended thallus to 1.0;
in the step 5: the resuspended cells were diluted with BHI Sup. medium at a ratio of 1:100, added to a 96-well plate at 200 ul/well, and anaerobically cultured at 37 ℃ for 120 h.
3. The method of claim 1, wherein the cryopreserved species of propionibacterium acnes is obtained from a lesion of the acne patient after isolation and identification.
4. The method of claim 1, wherein the harvested seed is placed in glycerol and stored at-80 ℃.
5. The method of claim 1, wherein the water is deionized water.
6. The method of claim 1, wherein the blood plate is a columbia blood agar plate.
7. The method of claim 1, wherein in step 3, the inoculation medium is Brookfield agar broth.
8. The method of claim 1, wherein in step 4, the medium used for resuspension is Brookfield agar broth.
9. The method for constructing and culturing a Propionibacterium acnes biofilm for experimental study as claimed in claim 1, wherein in said step 3, the culture environment is anaerobic.
10. The method for constructing and culturing a Propionibacterium acnes biofilm for experimental study as claimed in claim 1, wherein in said step 5, the culture environment is anaerobic.
11. The method according to any one of claims 1 to 10, wherein the yeast extract is a high-purity high-protein type yeast extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910737263.3A CN112391301A (en) | 2019-08-11 | 2019-08-11 | Construction and culture method of propionibacterium acnes biofilm for experimental research |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910737263.3A CN112391301A (en) | 2019-08-11 | 2019-08-11 | Construction and culture method of propionibacterium acnes biofilm for experimental research |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112391301A true CN112391301A (en) | 2021-02-23 |
Family
ID=74602120
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910737263.3A Pending CN112391301A (en) | 2019-08-11 | 2019-08-11 | Construction and culture method of propionibacterium acnes biofilm for experimental research |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112391301A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110684689A (en) * | 2019-10-22 | 2020-01-14 | 复旦大学附属华山医院 | Construction and culture method of propionibacterium acnes biofilm |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107105749A (en) * | 2014-08-13 | 2017-08-29 | 阿克索生物医药公司 | Antimicrobe compound and composition and application thereof |
| US20190105343A1 (en) * | 2016-03-24 | 2019-04-11 | Bioclenz Ph Llc | Treatment of Skin Conditions and Diseases Associated with Microbial Biofilms |
| WO2019113066A1 (en) * | 2017-12-05 | 2019-06-13 | Biomx Ltd. | Bacteriophage treatment for acne and biofilms |
| CN110684689A (en) * | 2019-10-22 | 2020-01-14 | 复旦大学附属华山医院 | Construction and culture method of propionibacterium acnes biofilm |
| CN112771149A (en) * | 2018-08-23 | 2021-05-07 | 韩国亿诺生物有限公司 | Novel Propionibacterium granulatus strain and composition for preventing or treating acne comprising the same or culture thereof |
-
2019
- 2019-08-11 CN CN201910737263.3A patent/CN112391301A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107105749A (en) * | 2014-08-13 | 2017-08-29 | 阿克索生物医药公司 | Antimicrobe compound and composition and application thereof |
| US20190105343A1 (en) * | 2016-03-24 | 2019-04-11 | Bioclenz Ph Llc | Treatment of Skin Conditions and Diseases Associated with Microbial Biofilms |
| WO2019113066A1 (en) * | 2017-12-05 | 2019-06-13 | Biomx Ltd. | Bacteriophage treatment for acne and biofilms |
| CN111867608A (en) * | 2017-12-05 | 2020-10-30 | 拜欧麦克斯有限公司 | Phage therapy for acne and biofilm |
| US20210177920A1 (en) * | 2017-12-05 | 2021-06-17 | Biomx Ltd. | Bacteriophage treatment for acne and biofilms |
| CN112771149A (en) * | 2018-08-23 | 2021-05-07 | 韩国亿诺生物有限公司 | Novel Propionibacterium granulatus strain and composition for preventing or treating acne comprising the same or culture thereof |
| CN110684689A (en) * | 2019-10-22 | 2020-01-14 | 复旦大学附属华山医院 | Construction and culture method of propionibacterium acnes biofilm |
Non-Patent Citations (4)
| Title |
|---|
| VALÉRIE BORREL等: "Adaptation of acneic and non acneic strains of Cutibacterium acnes to sebum-like environment", MICROBIOLOGYOPEN, 4 April 2019 (2019-04-04), pages 1 - 10 * |
| 翁心华等: "传染病学", 30 September 2009, 复旦大学出出版社, pages: 214 * |
| 马英等: "痤疮丙酸杆菌生物膜形成与抗菌药物耐药的相关性分析", 中国感染与化疗杂志, 20 November 2021 (2021-11-20), pages 703 - 707 * |
| 齐显龙等: "痤疮丙酸杆菌生物膜的构建", 中国麻风皮肤病杂志, 10 February 2007 (2007-02-10), pages 126 - 128 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110684689A (en) * | 2019-10-22 | 2020-01-14 | 复旦大学附属华山医院 | Construction and culture method of propionibacterium acnes biofilm |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2019000850A1 (en) | Dendrobium officinale endophytic fungi strain and extracellular polysaccharide produced thereby as well as extraction method and application of extracellular polysaccharide | |
| WO2018099366A1 (en) | Bacillus subtilis, culture method therefor and application thereof | |
| CN110387330B (en) | Freeze-drying method for improving survival rate of lactobacillus plantarum by using composite protective agent | |
| WO2012016445A1 (en) | Bacillus subtilis strain and uses thereof | |
| CN104845896B (en) | Produce the bacterial strain and method of Weilan gum | |
| CN115637235B (en) | Staphylococcus epidermidis with good antioxidant and anti-inflammatory effects and application thereof | |
| CN101372682B (en) | Construction method of Epinephelus fuscoguttatus fin cell line | |
| CN112457994A (en) | Method for promoting growth of chlorella pyrenoidosa by using volatile fatty acid | |
| CN112391301A (en) | Construction and culture method of propionibacterium acnes biofilm for experimental research | |
| CN102559559A (en) | Bacillus and method of producing hyaluronidase by employing the same | |
| CN111202739A (en) | Application of sophorolipid in preparing medicine for resisting propionibacterium acnes and biomembrane thereof | |
| CN102719502A (en) | Method for producing L-alanine by mutating lactate-production bacteria | |
| CN102071165A (en) | Method for improving biomass of lactic acid bacteria at low pH by adding glutamic acid | |
| CN110684689A (en) | Construction and culture method of propionibacterium acnes biofilm | |
| CN114032179B (en) | Industrial cannabis endophytic fungus for producing CBD and application thereof | |
| US3597325A (en) | Production of 3-(2-nitro-3-chlorophenyl)-4-chloro-pyrrole | |
| CN106319016B (en) | Method for preparing 11 α,17 α -hydroxyprogesterone | |
| CN109294920A (en) | A method of addition nano material induces haematococcus pluvialis efficient accumulation astaxanthin | |
| Krishnamoorthy et al. | Culture of Rhinosporidium seeberi: preliminary report | |
| CN104830736B (en) | One plant of Pediococcus pentosaceus and its application | |
| CN103275921A (en) | Method for improving cholate tolerance of lactobacillus | |
| CN113493743A (en) | Heterotrophic-dilution-photoinduction culture method for chlorella pyrenoidosa in desert | |
| CN103113214B (en) | Method for adjusting and controlling structure and shape of calcium oxalate crystals by using colibacillus secretion | |
| CN102251017A (en) | Method for identifying function and capability of cells for phagocytizing exogenous foreign bodies | |
| JPS62263196A (en) | 14-hydroxyerythromycin derivative and production thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |