CN112364710A - Plant electric signal classification and identification method based on deep learning algorithm - Google Patents

Plant electric signal classification and identification method based on deep learning algorithm Download PDF

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CN112364710A
CN112364710A CN202011125913.8A CN202011125913A CN112364710A CN 112364710 A CN112364710 A CN 112364710A CN 202011125913 A CN202011125913 A CN 202011125913A CN 112364710 A CN112364710 A CN 112364710A
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刘锴
刘鹏
赵娇娇
袁悦
吕途
袁佩
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Abstract

The invention discloses a plant electric signal classification and identification method based on a deep learning algorithm. Then, training and recognizing the two-dimensional vector by using an image recognition algorithm in deep learning, and judging the current life state of the plant; the method solves the problems that the existing plant life state judgment is delayed in information acquisition, long in period, and not suitable for rare variety evaluation due to destructive measurement. The method is suitable for various fields such as water-saving irrigation, seedling selection, environmental monitoring and the like.

Description

Plant electric signal classification and identification method based on deep learning algorithm
Technical Field
The invention belongs to the technical field of biological information, and particularly relates to a plant electric signal classification and identification method based on a deep learning algorithm.
Background
Since the twentieth century, the population growth, the waste of water resources, the land reduction and the soil degradation, ecological damage and environmental pollution caused by the excessive use of chemical fertilizers and pesticides have become serious day by day. Under the situation, on one hand, saline-alkali-tolerant and drought-tolerant crops are developed and cultivated, the utilization rate of barren soil is improved, and grain production is maintained; on the other hand, develop accurate irrigation technique, realize the rational utilization to the water resource, be the inevitable trend of wisdom agricultural development.
To develop, utilize and cultivate saline-alkali-tolerant and drought-tolerant crops, an effective crop stress resistance evaluation method and index are needed, and the method and index can sensitively and accurately reflect the state change of the crops under the environmental stress. The realization of the accurate irrigation technology also depends on the method and the index to accurately judge the water shortage state of the crops.
The traditional indexes for evaluating the crop state are mainly divided into two types, namely morphological indexes and physiological and biochemical indexes. But the determination of the form index is complicated, the workload is large, and the measurement period is long; physiological and biochemical indexes need to be subjected to destructive measurement, and the use amount of a sample is large, so that the method is not suitable for measuring rare varieties.
At present, the state of crops is judged by identifying and analyzing the appearance and the shape of plants by using a deep learning technology. However, the method can only effectively identify the plant when the appearance characteristics of the plant are obviously changed, the obtained information has hysteresis, the predicted identification rate is 60-70%, and the identification rate is low.
Disclosure of Invention
The invention aims to provide a plant electric signal classification and identification method based on a deep learning algorithm, and solves the problems of information acquisition lag, long period, destructive measurement and unsuitability for rare variety evaluation in the existing plant life state judgment.
The invention adopts the technical scheme that a plant electric signal classification and identification method based on a deep learning algorithm is implemented according to the following steps:
step 1, sampling the plant electric signal at equal time intervals to form a discrete signal sequence, wherein the discrete signal sequence is defined as D and the length is len (D);
step 2, decomposing the discrete signal sequence D by using an empirical mode decomposition algorithm,obtaining n intrinsic mode functions, and splicing the intrinsic mode functions into a Len (D) x n two-dimensional matrix vector tensor DS(ii) a Tensor D of two-dimensional matrix vectorSLabeled as normal signal;
step 3, for plants under environmental stress, adopting the same processing method as the step 1 and the step 2, and marking the obtained two-dimensional matrix vector tensor as a stressed signal Ds'; the two-dimensional tensors of the normal signal and the stressed signal jointly form a sample data set data;
step 4, dividing the data of the sample data set into a training set, a testing set and a verification set;
step 5, importing the training set into a deep learning network model for training, adjusting the hyper-parameters of the model, primarily evaluating the capability of the model, importing the verification set into a trained network for adjusting the hyper-parameters and monitoring whether the model is over-fitted to obtain an optimized model;
and 6, processing and classifying the new data needing to be identified by using the optimization model so as to identify the life state of the current plant.
The invention is also characterized in that:
step 1, the plant electric signal acquisition position is one of a plant leaf and a plant stem.
Step 2, decomposing by using an empirical mode decomposition algorithm to obtain n intrinsic mode functions, wherein the specific process is as follows:
finding out all maximum value points of data in the discrete signal sequence, and fitting to form an envelope curve e of the maximum value of the original discrete signal sequence by using a cubic spline interpolation function+(t), the upper envelope; finding out all minimum value points of data in the original discrete signal sequence in the same way, and fitting to form a minimum value envelope e of the discrete signal sequence-(t), the lower envelope; the mean value of the upper and lower envelope lines is denoted as m1(t), then:
Figure BDA0002733609120000031
respectively subtracting upper envelope mean value m and lower envelope mean value m from data in discrete signal sequence1(t) obtaining a new signal sequence with low frequencies removed
Figure BDA0002733609120000032
Comprises the following steps:
Figure BDA0002733609120000033
judgment of
Figure BDA0002733609120000034
Whether there is a positive local minimum and a negative local maximum, and if so, whether there is a positive local minimum and a negative local maximum
Figure BDA0002733609120000035
Is an intermediate state function, pair
Figure BDA0002733609120000036
Repeating the above steps;
after going through the "screening" for k times,
Figure BDA0002733609120000037
satisfy two conditions of imf, define
Figure BDA0002733609120000038
Is the first order imf component of the set of signals;
then there are:
Figure BDA0002733609120000039
subtracting c from the original discrete signal sequence D (t)1(t) obtaining a new signal r1(t) having:
r1(t)=D(t)-c1(t)
handle r1(t) repeating the above steps as a new "original signal" to obtain imf2Component c of (t)2(t) similarly, the nth order component c can be obtainedn(t),
The expression of the original discrete signal sequence d (t) is:
Figure BDA00027336091200000310
imf thereini(t) is the eigenmode function, rn(t) is the residual of the decomposition.
And 4, dividing the data set into a training set, a testing set and a verification set in a ratio of 3:1: 1.
In step 5, the training set and the verification set are transmitted to the deep learning network, and the number of training iterations is 1000-2000.
And 5, the deep learning network is a convolutional neural network.
The specific process of the step 6 is as follows:
performing empirical mode decomposition on the new data d to obtain a two-dimensional vector matrix, recording the two-dimensional vector matrix as data _ I, wherein the shape of the two-dimensional vector matrix is mxn, and expanding the two-dimensional vector matrix into a [1, N ] vector x, wherein N is mxn; calculating the connection weight and the bias according to the parameters of the optimization model, and calculating a predicted value by combining the following formula:
y_pred=wTx+b
w represents the weight of the connection between neurons, b represents the bias of the neuron connection, and y _ pred is a predicted value.
The invention has the beneficial effects that:
the invention relates to a plant electric signal classification and identification method based on a deep learning algorithm. Then, training and recognizing the two-dimensional vector by using an image recognition algorithm in deep learning, and judging the current life state of the plant; the method solves the problems that the existing plant life state judgment is delayed in information acquisition, long in period, and not suitable for rare variety evaluation due to destructive measurement. The method is suitable for various fields such as water-saving irrigation, seedling selection, environmental monitoring and the like.
Detailed Description
The present invention will be described in detail with reference to the following embodiments.
The invention relates to a plant electric signal classification and identification method based on a deep learning algorithm, which is implemented according to the following steps:
step 1, sampling the plant electric signal at equal time intervals to form a discrete signal sequence, wherein the discrete signal sequence is defined as D and the length is len (D);
the plant electric signal acquisition position is one of a plant leaf and a plant stem.
Step 2, decomposing the discrete signal sequence D by an empirical mode decomposition algorithm to obtain n intrinsic mode functions, and splicing the intrinsic mode functions into a Len (D) x n two-dimensional matrix vector tensor DS(ii) a Tensor D of two-dimensional matrix vectorSLabeled as normal signal;
the specific process of obtaining n intrinsic mode functions by decomposing with an empirical mode decomposition algorithm is as follows:
finding out all maximum value points of data in the discrete signal sequence, and fitting to form an envelope curve e of the maximum value of the original discrete signal sequence by using a cubic spline interpolation function+(t), the upper envelope; finding out all minimum value points of data in the original discrete signal sequence in the same way, and fitting to form a minimum value envelope e of the discrete signal sequence-(t), the lower envelope; the mean value of the upper and lower envelope lines is denoted as m1(t), then:
Figure BDA0002733609120000051
respectively subtracting upper envelope mean value m and lower envelope mean value m from data in discrete signal sequence1(t) obtaining a new signal sequence with low frequencies removed
Figure BDA0002733609120000052
Comprises the following steps:
Figure BDA0002733609120000053
judgment of
Figure BDA0002733609120000054
Whether there is a positive local minimum and a negative local maximum, and if so, whether there is a positive local minimum and a negative local maximum
Figure BDA0002733609120000055
Is an intermediate state function, pair
Figure BDA0002733609120000056
Repeating the above steps;
after going through the "screening" for k times,
Figure BDA0002733609120000057
satisfy two conditions of imf, define
Figure BDA0002733609120000058
Is the first order imf component of the set of signals;
then there are:
Figure BDA0002733609120000059
subtracting c from the original discrete signal sequence D (t)1(t) obtaining a new signal r1(t) having:
r1(t)=D(t)-c1(t)
handle r1(t) repeating the above steps as a new "original signal" to obtain imf2Component c of (t)2(t) similarly, the nth order component c can be obtainedn(t),
The expression of the original discrete signal sequence d (t) is:
Figure BDA0002733609120000061
imf thereini(t) is the eigenmode function, rn(t) is the residual of the decomposition.
Step 3, for plants under environmental stress, adopting the same processing method as the step 1 and the step 2, and marking the obtained two-dimensional matrix vector tensor as a stressed signal Ds'; the two-dimensional tensors of the normal signal and the stressed signal jointly form a sample data set data;
step 4, dividing the data of the sample data set into a training set, a testing set and a verification set;
the data set is divided into a training set, a testing set and a verification set in a ratio of 6:2: 2.
Step 5, importing the training set into a deep learning network model for training, adjusting the hyper-parameters of the model, primarily evaluating the capability of the model, importing the verification set into a trained network for adjusting the hyper-parameters and monitoring whether the model is over-fitted to obtain an optimized model;
the training set and the verification set are transmitted into a deep learning network, and the number of training iterations is 1000-2000.
The deep learning network is a convolutional neural network.
Step 6, using the optimization model to process and classify the new data to be identified, thereby identifying the life state of the current plant;
the specific process of the step 6 is as follows:
performing empirical mode decomposition on the new data d to obtain a two-dimensional vector matrix, recording the two-dimensional vector matrix as data _ I, wherein the shape of the two-dimensional vector matrix is mxn, and expanding the two-dimensional vector matrix into a [1, N ] vector x, wherein N is mxn; calculating the connection weight and the bias according to the parameters of the optimization model, and calculating a predicted value by combining the following formula:
y_pred=wTx+b
w represents the weight of the connection between neurons, b represents the bias of the neuron connection, and y _ pred is a predicted value.
The invention relates to a plant electric signal classification and identification method based on a deep learning algorithm, which has the working principle that:
in the life activity of plants, there is fluctuation of electric potential, i.e., electric signal, which is a comprehensive reflection of the electrophysiological activity of cell groups highly related to plant cells, and is closely related to the physiological state of plant cells. In the case where the plant is not affected by external factors, the plant leaf electrical signal is considered to be a normal signal. When a plant is stimulated (such as drought and saline-alkali stress), action potential and variable potential are generated by cells, and are rapidly transmitted in tissues and organs at a relatively high speed, and the electrical signals of plant leaves are changed and taken as abnormal signals influenced by stimulation factors. The electrical signal of a plant is the most direct and rapid manifestation of its life state. The signals are accurately identified, so that the current life state of the plant can be sensitively detected. The method can be used in the fields of automatic irrigation control, selection and breeding of saline-alkali tolerant and drought resistant varieties and the like.
Examples
Collecting electric signals of leaves of the normally growing corn seedlings, wherein the electric signals are divided into one group in 10 minutes, and the total number of the groups is 500; 500 groups of leaf electric signals under saline-alkali stress and drought stress are collected by the same method, each signal is randomly divided into a training set, a testing set and a verification set according to the ratio of 6:2:2, and corresponding labels are marked. The method comprises the following specific steps:
normal maize signal label: [100]
corn saline-alkali stress signals: [010]
maize drought stress signals: [001]
and decomposing the data by adopting an empirical mode decomposition algorithm (EMD) to obtain intrinsic mode functions of each group of signals, and splicing the intrinsic mode functions to obtain a two-dimensional tensor D corresponding to each growth state. And combining the three states D to obtain a data set I.
Sampling the two-dimensional matrix in the I by using a convolution method, performing pooling processing on data by using a max _ posing method to obtain high-dimensional characteristics of the data, and using a data set I1To indicate.
Data set I1Forward propagation is performed with a learning rate of 10-3And iterating for 1000-1500 times, and adjusting the hyper-parameter by using a back propagation algorithm (BP algorithm).
And leading the verification set into a trained neural network to verify the recognition rate. If the recognition rate is higher, the obtained weights and biases of the training are retained. If the recognition rate is low, the learning rate and the iteration times are adjusted, and finally a matrix with the weight and the bias of 3 x 128 is obtained.
And performing convolution pooling on the data to be identified, calculating a predicted value y _ pred by using the weight and the bias obtained by the deep learning network, and classifying the input data according to the y _ pred.
The recognition rate is calculated according to the classification result and is 86%, compared with the existing prediction recognition rate of 60-70%, the recognition rate of the method is obviously improved.
Through the mode, the method adopts a variable modal decomposition algorithm with self-adaptive characteristics, and by collecting the plant leaf electric signals and adopting a two-dimensional decomposition algorithm, the one-dimensional electric signals are expanded into two-dimensional vectors which are distributed from low frequency to high frequency in sequence. Then, training and recognizing the two-dimensional vector by using an image recognition algorithm in deep learning, and judging the current life state of the plant; the method solves the problems that the existing plant life state judgment is delayed in information acquisition, long in period, and not suitable for rare variety evaluation due to destructive measurement. The method is suitable for various fields such as water-saving irrigation, seedling selection, environmental monitoring and the like.

Claims (7)

1. A plant electric signal classification and identification method based on a deep learning algorithm is characterized by comprising the following steps:
step 1, sampling the plant electric signal at equal time intervals to form a discrete signal sequence, wherein the discrete signal sequence is defined as D and the length is len (D);
step 2, decomposing the discrete signal sequence D by an empirical mode decomposition algorithm to obtain n intrinsic mode functions, and splicing the intrinsic mode functions into a Len (D) x n two-dimensional matrix vector tensor DS(ii) a Tensor D of two-dimensional matrix vectorSLabeled as normal signal;
step 3, for plants under environmental stress, adopting the same processing method as the step 1 and the step 2, and marking the obtained two-dimensional matrix vector tensor as a stressed signal Ds'; the two-dimensional tensors of the normal signal and the stressed signal jointly form a sample data set data;
step 4, dividing the data of the sample data set into a training set, a testing set and a verification set;
step 5, importing the training set into a deep learning network model for training, adjusting the hyper-parameters of the model, primarily evaluating the capability of the model, importing the verification set into a trained network for adjusting the hyper-parameters and monitoring whether the model is over-fitted to obtain an optimized model;
and 6, processing and classifying the new data needing to be identified by using the optimization model so as to identify the life state of the current plant.
2. The plant electrical signal classification and identification method based on the deep learning algorithm as claimed in claim 1, wherein the plant electrical signal collection position in step 1 is one of a plant leaf and a plant stem.
3. The deep learning algorithm-based plant electrical signal classification and identification method according to claim 1, wherein the empirical mode decomposition algorithm is used for decomposing in the step 2, and the specific process of obtaining n intrinsic mode functions is as follows:
finding out all maximum value points of data in the discrete signal sequence, and fitting to form an envelope curve e of the maximum value of the original discrete signal sequence by using a cubic spline interpolation function+(t), the upper envelope; finding out all minimum value points of data in the original discrete signal sequence in the same way, and fitting to form a minimum value envelope e of the discrete signal sequence-(t), the lower envelope; the mean value of the upper and lower envelope lines is denoted as m1(t), then:
Figure FDA0002733609110000021
respectively subtracting upper envelope mean value m and lower envelope mean value m from data in discrete signal sequence1(t) obtaining a new signal sequence with low frequencies removed
Figure FDA0002733609110000022
Comprises the following steps:
Figure FDA0002733609110000023
judgment of
Figure FDA0002733609110000024
Whether there is a positive local minimum and a negative local maximum, and if so, whether there is a positive local minimum and a negative local maximum
Figure FDA0002733609110000025
Is an intermediate state function, pair
Figure FDA0002733609110000026
Repeating the above steps;
after going through the "screening" for k times,
Figure FDA0002733609110000027
satisfy two conditions of imf, define
Figure FDA0002733609110000028
Is the first order imf component of the set of signals;
then there are:
Figure FDA0002733609110000029
subtracting c from the original discrete signal sequence D (t)1(t) obtaining a new signal r1(t) having:
r1(t)=D(t)-c1(t)
handle r1(t) repeating the above steps as a new "original signal" to obtain imf2Component c of (t)2(t) similarly, the nth order component c can be obtainedn(t),
The expression of the original discrete signal sequence d (t) is:
Figure FDA00027336091100000210
imf thereini(t) is the eigenmode function, rn(t) is the residual of the decomposition.
4. The deep learning algorithm-based plant electric signal classification and identification method as claimed in claim 1, wherein the data set is divided into a training set, a test set and a verification set in a ratio of 3:1:1 in step 4.
5. The method as claimed in claim 1, wherein the training set and the verification set are introduced into the deep learning network in step 5, and the number of training iterations is 1000-2000.
6. The plant electric signal classification and identification method based on the deep learning algorithm as claimed in claim 1, wherein the deep learning network in step 5 is a convolutional neural network.
7. The deep learning algorithm-based plant electric signal classification and identification method according to claim 1, wherein the specific process of step 6 is as follows:
performing empirical mode decomposition on the new data d to obtain a two-dimensional vector matrix, recording the two-dimensional vector matrix as data _ I, wherein the shape of the two-dimensional vector matrix is mxn, and expanding the two-dimensional vector matrix into a [1, N ] vector x, wherein N is mxn; calculating the connection weight and the bias according to the parameters of the optimization model, and calculating a predicted value by combining the following formula:
y_pred=wTx+b
w represents the weight of the connection between neurons, b represents the bias of the neuron connection, and y _ pred is a predicted value.
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