CN112352793B - Metarhizium anisopliae granules stored for long time - Google Patents
Metarhizium anisopliae granules stored for long time Download PDFInfo
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- CN112352793B CN112352793B CN202011320186.0A CN202011320186A CN112352793B CN 112352793 B CN112352793 B CN 112352793B CN 202011320186 A CN202011320186 A CN 202011320186A CN 112352793 B CN112352793 B CN 112352793B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
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- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Toxicology (AREA)
- Microbiology (AREA)
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- Biotechnology (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of microorganisms, and particularly relates to a metarhizium anisopliae granule stored for a long time. The invention can obviously prolong the shelf life of the metarhizium anisopliae granules and can obviously improve the storage performance of the metarhizium anisopliae granules.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to a metarhizium anisopliae granule stored for a long time.
Background
Pesticides are chemicals and biopharmaceuticals used to control pests that harm agriculture, forestry, and animal husbandry, and to regulate plant growth. The pesticide is an essential production data in agricultural production, and plays an important role in protecting crops and harvested products from being damaged by harmful organisms, improving the inferior resistance of the crops and promoting the agricultural yield increase. For a long time, chemical pesticides have been the main agents for controlling crop pests.
However, with the wide application of chemical pesticides in the prevention and control of crop diseases and insect pests, the environmental pollution and public nuisance caused by the chemical pesticides are increasingly serious, and the accumulation of pesticides in the food chain caused by drug residues is not easy to control, so that a large number of drug-induced diseases are caused; in addition, the killing and poisoning effects of chemical pesticides on non-target organisms seriously affect the natural ecological balance and the self-regulation capacity of an ecological system, and the drug resistance of the target organisms is increasingly enhanced (the current research and application situation of microbial pesticides in China, Wangyi and the like, Sichuan food and fermentation, No. 42, No. 5, No. 1, No. 8, No. 1, No. 4, No. 1, No. 11/29 in published date, No. 1, No. 4 in No. 1, No. 11/29 in published date, biological pesticide research dynamics, trends and prospect, Liuqing Shu and the like, pesticide research and application, No. 11, No. 2, No. 1, No. 8 in No. 17, No. 1, No. 2/28 in published date, 2007).
The biological pesticide is biological living body with pesticide property for preventing and treating diseases, pests, grass and other harmful organisms, and the generated physiologically active substance and transgenic product, including microbial pesticide, botanical pesticide, biochemical pesticide, transgenic biological pesticide, natural enemy organism and the like. Compared with chemical pesticides, biological pesticides have the advantages of safety to human and livestock and non-target organisms, good environmental compatibility, difficulty in generating resistance, easiness in protecting biological diversity, wide sources and the like (the current situation and development prospect of biological pesticide research and application, the Chamingshan and the like, Shenyang agriculture university bulletin, Vol.37, No. 4, p.545, No. 1, lines 1-4, and published 2006, 8, 31).
Wherein the microbial pesticide is a microbial living preparation for preventing and treating diseases, insects, grass and other harmful organisms, and mainly comprises a microbial bactericide, a microbial pesticide and a microbial herbicide (the current condition and development prospect of biological pesticide research and application, Chamingshan and the like, Shenyang agriculture university bulletin, Vol. 37, No. 4, p. 545, No. 2, lines 1-2, and published 2006, 8 and 31).
Metarhizium anisopliae is a broad spectrum of entomopathogenic bacteria distributed in tropical and subtropical regions, and the entomogenous fungi are propagated by reproduction and budding. According to statistics, the number of the metarhizium anisopliae host insects can reach more than 200. Metarhizium anisopliae can induce insects to produce metarhizium anisopliae, and can form repeated infection in insect populations. In order to facilitate product storage, field application and improve control effect, the pesticide composition is generally processed into various dosage forms. The granules are an ideal dosage form for preventing and controlling underground pests, are convenient to throw and have more important functions of providing a microenvironment beneficial to survival and colonization for the metarhizium anisopliae, thereby improving the stress resistance of the granules to the environment and the preventing and controlling effect on the pests. Entomogenous fungi granules comprise entomogenous fungi granules, and comprise a granule carrier and entomogenous fungi spores. The particle carriers are divided into mineral carriers, plant carriers and synthetic carriers according to the sources, and can also be fermented natural crop particles or artificially synthesized nutritional particles; due to the particularity of the fungus granules, the problems of low survival activity of thalli, short storage shelf life, poor field colonization and the like exist. During transport, storage and use of fungal granules, there is a need to take into account the problem of spore survival in the environment.
Shelf life is an important index for measuring the quality of all fungal insecticides. Shelf life is an important index for measuring the quality of all fungal insecticides. The effective infection of the fungal pesticide has to have more than one year of storage period before the fungal pesticide has commercial development value.
However, the shelf life of the existing metarhizium anisopliae granules is short.
Disclosure of Invention
In view of the above, the present invention aims to provide a composition comprising an antioxidant, a water retention agent and glycerol for prolonging the shelf life of metarhizium anisopliae granules.
Except for special instructions, the parts are parts by mass.
In order to achieve the purpose, the technical scheme of the invention is as follows:
use of a composition comprising an antioxidant, a water retention agent and glycerol to prolong the shelf life of metarhizium anisopliae granules.
During the research process, the inventor unexpectedly finds that the composition containing the antioxidant, the water retention agent and the glycerol can remarkably prolong the shelf life of the metarhizium anisopliae granules.
Further, the mass ratio of the antioxidant to the water-retaining agent to the glycerol is 5-15:10-20: 65-85.
Further, the mass ratio of the metarhizium anisopliae granules to the antioxidant to the water-retaining agent to the glycerol is 1000:10:15: 75.
Further, the antioxidant comprises 1-2 of tert-butyl hydroquinone, butyl hydroxy anisol and di-tert-butyl hydroxy toluene.
Further, the water-retaining agent comprises a high-molecular water-absorbing resin.
Further, the metarhizium anisopliae is metarhizium anisopliae.
The invention also aims at a composition for protecting Metarrhizium anisopliae comprising: antioxidant, water retention agent and glycerin.
Further, the mass ratio of the antioxidant to the water-retaining agent to the glycerol is 5-15:10-20: 65-85.
Further, the mass ratio of the antioxidant to the water-retaining agent to the glycerin is 10:15: 75.
Further, the antioxidant comprises 1-2 of tert-butyl hydroquinone, butyl hydroxy anisol and di-tert-butyl hydroxy toluene.
Further, the water-retaining agent comprises a high-molecular water-absorbing resin.
Further, the metarhizium anisopliae is metarhizium anisopliae.
The invention has the beneficial effects that:
the invention can obviously improve the storage performance of the metarhizium anisopliae granules, and the spore germination rate can reach more than 40 percent after accelerated storage for 12 months at 30 ℃ under the non-vacuum storage condition.
The shelf life of the composition of the invention is significantly extended, which can be up to more than 12 months.
The method is simple and is beneficial to realizing industrial production.
Detailed Description
The examples are provided for better illustration of the present invention, but the present invention is not limited to the examples. Therefore, those skilled in the art should make insubstantial modifications and adaptations to the embodiments of the present invention in light of the above teachings and remain within the scope of the invention.
The following metarhizium anisopliae (CQMa421) granules were provided by Chongqing Jiulixin bioengineering ltd, and had a spore content of 2 hundred million active spores/g.
The following polymeric water-absorbent resins were purchased from Xin ya environmental protection materials Co.
Comparative example 1
Packaging the metarhizium anisopliae (CQMa421) granule per 200g in aluminum foil bag, and sealing by heat treatment.
Comparative example 2
Putting Metarhizium anisopliae (CQMa421) granule per 200g into aluminum foil bag, vacuum-treating with 450 type vacuum packaging machine until the vacuum degree reaches 0.04Mpa, and heat-treating and sealing.
Example 1
Taking 5 parts of tert-butyl hydroquinone (TBHQ), 10 parts of water-absorbent resin and 85 parts of 50% glycerol-water mixed solution according to the weight ratio, uniformly mixing, and mixing the obtained mixture with metarhizium anisopliae (CQMa421) granules according to the mass ratio of 1: 10, mixing evenly, filling 200g of the mixture into an aluminum foil bag, and sealing the aluminum foil bag by heat treatment.
Example 2
Uniformly mixing 5 parts of tert-butyl hydroquinone (TBHQ), 10 parts of water-absorbent resin and 85 parts of 50% glycerol-water mixed solution by volume, and mixing the obtained mixture with metarhizium anisopliae (CQMa421) granules according to the mass ratio of 1: 10, uniformly mixing, filling 200g of the mixture into an aluminum foil bag, performing vacuum treatment by using a 450 type vacuum packaging machine until the vacuum degree reaches 0.04Mpa, and performing heat treatment and sealing.
Example 3
Uniformly mixing 10 parts of tert-butyl hydroquinone (TBHQ), 15 parts of water-absorbent resin and 75 parts of 50% glycerol-water mixed solution, and mixing the obtained mixture with metarhizium anisopliae (CQMa421) granules according to a mass ratio of 1: 10, mixing evenly, filling 200g of the mixture into an aluminum foil bag, and sealing the aluminum foil bag by heat treatment.
Example 4
Mixing 10 parts of tert-butyl hydroquinone (TBHQ), 15 parts of water-absorbent resin and 75 parts of 50% glycerol-water mixed solution, and mixing the obtained mixture with metarhizium anisopliae (CQMa421) granules according to a mass ratio of 1: 10, uniformly mixing, filling 200g of the mixture into an aluminum foil bag, performing vacuum treatment by using a 450 type vacuum packaging machine until the vacuum degree reaches 0.04Mpa, and performing heat treatment and sealing.
Example 5
Mixing 10 parts of tert-butyl hydroquinone (TBHQ), 15 parts of water-absorbent resin and 75 parts of 50% glycerol-water mixed solution, and mixing the obtained mixture with metarhizium anisopliae (CQMa421) granules according to a mass ratio of 1: 10, uniformly mixing, drying in vacuum, filling 200g of the mixture into an aluminum foil bag, and carrying out heat treatment and sealing.
Example 6
Taking 15 parts of tert-butyl hydroquinone (TBHQ), 20 parts of water-absorbent resin and 65 parts of 50% glycerol-water mixed solution, mixing the obtained mixture with metarhizium anisopliae (CQMa421) granules according to the mass ratio of 1: 10 g of the mixture is uniformly mixed, every 200g of the mixture is put into an aluminum foil bag, a 450 type vacuum packaging machine is used for vacuum treatment, and when the vacuum degree reaches 0.04Pa, the aluminum foil bag is subjected to heat treatment and sealed.
Determination of storage period of metarhizium anisopliae CQMa421 particles
The shelf life of the products obtained in comparative examples 1-2 and examples 1-6 was examined and specifically: the obtained product is stored in an accelerated manner under the constant conditions that the temperature is 30 ℃ and the air humidity is 60 percent; the spore germination rate of the product is measured at 0, 3, 6, 9 and 12 months of storage respectively, and the result is shown in table 1;
the spore germination rate detection method comprises the following steps: the incubated samples were formulated as 2X 107spore/mL spore suspension, 100uL coated 1/4SDAY plate; culturing at a constant temperature of (26 +/-1) ° C for 24h, scratching a (0.5-1) cm multiplied by (0.5-1) cm agar block, dyeing the agar block with a lapachol lactate staining solution, observing a flat plate under an optical microscope, counting the number of germinated spores and ungerminated spores in a visual field, and counting the germinated spores by using the spores with the spore germination length being greater than the spore radius or 1/2 long axis length; observing each plate for n times of visual field (n is more than or equal to 3), observing the total number of spores to be more than 300, and calculating the germination rate [ (the number of germinated spores/the number of observed total spores) × 100%]。
TABLE 1 shelf life measurement results
Remarking: capital differences indicate significant differences at the 0.01 level.
As can be seen from Table 1, the shelf lives of the products of examples 1-6 were significantly extended compared to those of comparative examples 1-2. Therefore, the composition provided by the invention can obviously prolong the shelf life of the metarhizium anisopliae granules and can obviously improve the storage performance of the metarhizium anisopliae granules.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (2)
1. The application of a composition comprising 10 parts by mass of an antioxidant, 15 parts by mass of a high-molecular water-absorbent resin and 75 parts by mass of a 50vol% glycerol-water mixture in prolonging the shelf life of metarhizium anisopliae granules; the antioxidant comprises 1-2 of tert-butyl hydroquinone, butyl hydroxy anisol and di-tert-butyl hydroxy toluene.
2. The storage-resistant composition comprises the following components in parts by mass: 10 parts of antioxidant, 15 parts of high-molecular water-absorbing resin and 75 parts of 50vol% glycerol-water mixture, wherein the oxidant comprises 1-2 of tert-butyl hydroquinone, butyl hydroxy anisol and di-tert-butyl hydroxy toluene.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011320186.0A CN112352793B (en) | 2020-11-23 | 2020-11-23 | Metarhizium anisopliae granules stored for long time |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011320186.0A CN112352793B (en) | 2020-11-23 | 2020-11-23 | Metarhizium anisopliae granules stored for long time |
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| CN112352793A CN112352793A (en) | 2021-02-12 |
| CN112352793B true CN112352793B (en) | 2021-11-05 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1095758A (en) * | 1993-05-17 | 1994-11-30 | 艾克科技公司 | Storing fungal cultures and conidial method |
| CN1329829A (en) * | 2001-08-20 | 2002-01-09 | 西南农业大学 | Method for storing conidia liquid of insecticiding funguses |
| CN107254286A (en) * | 2017-06-27 | 2017-10-17 | 六安三创建筑材料有限公司 | A kind of universal environment protection architecture glue and preparation method thereof |
| CN108977361A (en) * | 2018-08-03 | 2018-12-11 | 北京泰克美高新技术有限公司 | gel-type microbial inoculum and preparation method thereof |
| WO2019238734A1 (en) * | 2018-06-15 | 2019-12-19 | Bayer Aktiengesellschaft | Liquid and storage-stable formulations for fungal spores |
-
2020
- 2020-11-23 CN CN202011320186.0A patent/CN112352793B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1095758A (en) * | 1993-05-17 | 1994-11-30 | 艾克科技公司 | Storing fungal cultures and conidial method |
| CN1329829A (en) * | 2001-08-20 | 2002-01-09 | 西南农业大学 | Method for storing conidia liquid of insecticiding funguses |
| CN107254286A (en) * | 2017-06-27 | 2017-10-17 | 六安三创建筑材料有限公司 | A kind of universal environment protection architecture glue and preparation method thereof |
| WO2019238734A1 (en) * | 2018-06-15 | 2019-12-19 | Bayer Aktiengesellschaft | Liquid and storage-stable formulations for fungal spores |
| CN108977361A (en) * | 2018-08-03 | 2018-12-11 | 北京泰克美高新技术有限公司 | gel-type microbial inoculum and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| "金龟子绿僵菌种衣剂的研制";杨震元;《中国优秀硕士学位论文全文数据库 农业科技辑》;20100715(第7期);D046-86 * |
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