CN112342141A

CN112342141A - Stem cell culture equipment

Info

Publication number: CN112342141A
Application number: CN202011240689.7A
Authority: CN
Inventors: 齐雯歆
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-11-09
Filing date: 2020-11-09
Publication date: 2021-02-09

Abstract

The invention discloses stem cell culture equipment which comprises an incubator, wherein a sealing cover is arranged on the front surface of the incubator, two clamping grooves are formed in the front surface of the incubator, two buckles are fixedly connected to the inner side of the sealing cover, an installation box is fixedly installed inside the incubator, a motor is fixedly installed at the inner bottom of the installation box, a fixed frame body is fixedly installed at the inner bottom of the installation box, a first bevel gear is fixedly connected to one side, penetrating through the fixed frame body, of the output end of the motor, and the outer surface of the first bevel gear is connected with a second bevel gear in a meshed mode. Through placing the culture ware in the inside of fixed standing groove, the culture ware also can rotate thereupon when the second carousel rotates to make the inside culture solution of culture ware be in the circular motion state, prevent that the culture solution from taking place to solidify when static placing, thereby make stem cell keep the activity for a long time, then make the culture time improve.

Description

Stem cell culture equipment

Technical Field

The invention belongs to the technical field of culture equipment, and particularly relates to stem cell culture equipment.

Background

In brief, a stem cell is a type of cell that has unlimited or immortal self-renewal capacity, and is capable of producing at least one type of highly differentiated progeny cell. The definition of stem cells has been revised and defined from different levels over the years. Most biologists and physicians now believe that stem cells are a class of cells derived from embryonic, fetal, or adult cells that have the ability to self-renew and proliferate and differentiate without restriction under certain conditions, and that are capable of producing daughter cells with the same phenotype and genotype as themselves, and also capable of producing specialized cells that make up body tissues and organs, and also capable of differentiating into progenitor cells.

The development and progress of modern medicine are not independent of stem cell culture, stem cells are generally cultured in a culture vessel and then placed in an incubator, and the incubator is always placed statically, so that culture solution can be gradually solidified in the culture vessel, stem cells gradually lose activity, stem cell culture time cannot be too long, and the practicability of stem cell culture equipment in use is reduced.

Disclosure of Invention

In order to overcome the defects of the prior art, the invention provides stem cell culture equipment, a culture vessel is placed in a fixed placing groove, and the culture vessel rotates along with the second rotating disc when the second rotating disc rotates, so that the culture solution in the culture vessel is in a circular motion state, the culture solution is prevented from being solidified when being placed statically, stem cells keep activity for a long time, and the culture time is prolonged.

In order to solve the technical problems, the invention provides the following technical scheme: a stem cell culture device comprises an incubator, wherein a sealing cover is arranged on the front surface of the incubator, two clamping grooves are formed in the front surface of the incubator, two buckles are fixedly connected to the inner side of the sealing cover, an installation box is fixedly installed in the incubator, a motor is fixedly installed at the inner bottom of the installation box, a fixed frame body is fixedly installed at the inner bottom of the installation box, a first bevel gear is fixedly connected to one side, penetrating through the fixed frame body, of the output end of the motor, a second bevel gear is connected to the outer surface of the first bevel gear in a meshed mode, a rotating rod is connected to the top of the fixed frame body in a penetrating mode, the bottom end of the rotating rod is rotatably connected with the inner bottom of the installation box, a first rotating disc is fixedly connected to the top end of the rotating rod, a fixed sliding rod is fixedly connected to the top, the top end of the fixed sliding rod is fixedly connected with a second rotary table.

As a preferred technical scheme of the invention, four fixed placing grooves are fixedly mounted at the top of the second turntable, a culture vessel is arranged in each fixed placing groove, a pull rod penetrates through one side of each fixed placing groove, a fixed clamping plate is fixedly connected to the top end of each pull rod, an inner wall of each fixed placing groove is fixedly connected with a telescopic rod, the other end of each telescopic rod is fixedly connected with the outer side of the corresponding fixed clamping plate, and a reset spring is arranged on the outer surface of each telescopic rod.

As a preferred technical scheme of the invention, the rotating rod is fixedly sleeved with the second bevel gear, and a rotating shaft is arranged at the contact end of the bottom end of the rotating rod and the installation box.

As a preferred technical scheme of the invention, the fixed sliding rod is in sliding connection with the sliding groove, and the sliding groove and the fixed sliding rod are both circularly arranged.

As a preferable technical scheme of the invention, a bearing is arranged at the contact end of the outer surface of the rotating rod and the inside of the fixed frame body, and the second rotating disc and the first rotating disc are both circularly arranged.

As a preferable technical solution of the present invention, one side of the return spring is fixedly connected to an inner wall of the fixed placement groove, and the other side of the return spring is fixedly connected to an outer side of the fixed clamp plate.

As a preferable technical scheme of the invention, the rubber pad is arranged on the inner side of the fixed splint and is arranged in an arc shape.

Compared with the prior art, the invention can achieve the following beneficial effects:

1. by placing the culture vessel in the fixed placing groove, the culture vessel can also rotate along with the second turntable when the second turntable rotates, so that the culture solution in the culture vessel is in a circular motion state, the culture solution is prevented from being solidified when placed statically, stem cells can keep activity for a long time, and the culture time is prolonged;

2. through setting up fixing device to fix the culture ware, prevent that fixed standing groove from rotating when too fast, cause the culture ware to drop from the inside of fixed standing groove, realized then protecting the culture ware, and reduced the landing and to the damage that the culture ware caused.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic cross-sectional front view of the present invention;

FIG. 3 is a schematic top view of the incubator of the present invention;

FIG. 4 is an enlarged view of the structure of FIG. 3 according to the present invention;

fig. 5 is a schematic top view of the installation box of the present invention.

Wherein: 1. an incubator; 2. a sealing cover; 3. buckling; 4. a card slot; 5. installing a box; 6. a motor; 7. a first bevel gear; 8. a second bevel gear; 9. a fixed frame body; 10. rotating the rod; 11. a first turntable; 12. a chute; 13. fixing the sliding rod; 14. a second turntable; 15. fixing the placing groove; 16. a culture vessel; 17. a pull rod; 18. fixing the clamping plate; 19. a telescopic rod; 20. a return spring.

Detailed Description

The present invention will be further described with reference to specific embodiments for the purpose of facilitating an understanding of technical means, characteristics of creation, objectives and functions realized by the present invention, but the following embodiments are only preferred embodiments of the present invention, and are not intended to be exhaustive. Based on the embodiments in the implementation, other embodiments obtained by those skilled in the art without any creative efforts belong to the protection scope of the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified, and materials, reagents and the like used in the following examples are commercially available unless otherwise specified.

Example (b):

as shown in figures 1-5, a stem cell culture device comprises an incubator 1, a sealing cover 2 is arranged on the front surface of the incubator 1, two clamping grooves 4 are arranged on the front surface of the incubator 1, two buckles 3 are fixedly connected to the inner side of the sealing cover 2, an installation box 5 is fixedly installed inside the incubator 1, a motor 6 is fixedly installed at the inner bottom of the installation box 5, a fixed frame body 9 is fixedly installed at the inner bottom of the installation box 5, a first bevel gear 7 is fixedly connected to one side, through which an output end of the motor 6 penetrates the fixed frame body 9, a second bevel gear 8 is connected to the outer surface of the first bevel gear 7 in a meshing manner, a rotating rod 10 is connected to the top of the fixed frame body 9 in a penetrating manner, the bottom of the rotating rod 10 is rotatably connected with the inner bottom of the installation box 5, a first rotating disk 11 is fixedly connected to the top of the, the top of the mounting box 5 is provided with a sliding chute 12, and the top end of a fixed sliding rod 13 is fixedly connected with a second rotary table 14;

firstly, the culture vessel 16 is placed in the incubator 1, the sealing cover 2 is closed, the buckle 3 is clamped with the clamping groove 4, then, through the internal sealing performance of the incubator 1, under the action of an external power supply, the motor 6 is rotated, the motor 6 drives the first bevel gear 7 to rotate when rotating, the first bevel gear 7 drives the second bevel gear 8 to rotate when rotating, the second bevel gear 8 drives the rotating rod 10 to rotate when rotating, the fixed slide rod 13 is driven to rotate when the rotating rod 10 rotates, the fixed slide rod 13 is connected with the fixed slide rod 13 in a sliding way through the sliding groove 12, when the fixed slide rod 13 rotates, the second rotary disk 14 is driven to rotate, the stability of the second rotary disk 14 is improved, when the second rotary disk 14 rotates, the culture vessel 16 is made to rotate in a circular way, by placing the culture vessel 16 in the fixed placing groove 15, when the second rotary disk 14 rotates, the culture vessel 16 also rotates along with the fixed placing groove, thereby, the culture solution in the culture vessel 16 is in a circular motion state, the culture solution is prevented from being solidified when the culture solution is placed statically, the stem cells keep activity for a long time, and the culture time is prolonged.

In other embodiments, four fixed placing grooves 15 are fixedly installed at the top of the second rotating disc 14, a culture vessel 16 is arranged inside the fixed placing grooves 15, a drawing rod 17 penetrates through one side of the fixed placing grooves 15, a fixed clamping plate 18 is fixedly connected to the top end of the drawing rod 17, an expansion link 19 is fixedly connected to the inner wall of the fixed placing grooves 15, the other end of the expansion link 19 is fixedly connected to the outer side of the fixed clamping plate 18, and a return spring 20 is arranged on the outer surface of the expansion link 19;

make solid fixed splint 18 shrink through pulling pull pole 17, place the inside at fixed standing groove 15 with cultivateing household utensils 16, loosening pull pole 17, under reset spring 20's elastic deformation, make reset spring 20 promote solid fixed splint 18 and remove, thereby fix cultivateing household utensils 16, when fixed standing groove 15 carries out the circumferencial rotation, cultivateing household utensils 16 is still stable on fixed standing groove 15's surface, through setting up fixing device, so that fix cultivateing household utensils 16, prevent that fixed standing groove 15 from rotating when too fast, cause cultivateing household utensils 16 and drop from fixed standing groove 15's inside, then realized protecting cultivateing household utensils 16, and reduce the damage that the landing caused cultivateing household utensils 16.

In other embodiments, the rotating rod 10 is fixedly sleeved with the second bevel gear 8, and a rotating shaft is arranged at the contact end of the bottom end of the rotating rod 10 and the installation box 5; by providing the rotation shaft, stability when the rotation lever 10 is rotated is improved, and a positional deviation when the rotation lever 10 is rotated is prevented.

In other embodiments, the fixed sliding rod 13 is slidably connected to the sliding chute 12, and both the sliding chute 12 and the fixed sliding rod 13 are arranged in a circle; so as to limit the fixed slide bar 13, and then improve the stability of the rotation of the second rotary table 14 when the fixed slide bar 13 drives the second rotary table 14 to rotate.

In other embodiments, a bearing is arranged at the contact end between the outer surface of the rotating rod 10 and the inside of the fixed frame body 9, and the second rotating disc 14 and the first rotating disc 11 are both circularly arranged; through setting up the bearing to stability when improving dwang 10 and rotating to and play the supporting effect to dwang 10, take place to drop when preventing then that dwang 10 from rotating.

In other embodiments, one side of the return spring 20 is fixedly connected with the inner wall of the fixed placing groove 15, and the other side of the return spring 20 is fixedly connected with the outer side of the fixed clamping plate 18; the return spring 20 pushes the fixing clamp 18 through the elastic deformation of the return spring 20, so that the fixing clamp 18 fixes the culture vessel 16.

In other embodiments, the inner side of the fixed splint 18 is provided with a rubber pad, and the rubber pad is arranged in an arc shape; by providing a rubber mat to protect the culture dish 16, the culture dish 16 is prevented from being worn when the culture dish 16 is fixed.

In the present invention, unless otherwise expressly stated or limited, "above" or "below" a first feature means that the first and second features are in direct contact, or that the first and second features are not in direct contact but are in contact with each other via another feature therebetween. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and the preferred embodiments of the present invention are described in the above embodiments and the description, and are not intended to limit the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. A stem cell culture apparatus comprising an incubator (1), characterized in that: the front surface of the incubator (1) is provided with a sealing cover (2), the front surface of the incubator (1) is provided with two clamping grooves (4), the inner side of the sealing cover (2) is fixedly connected with two buckles (3), the inner part of the incubator (1) is fixedly provided with an installation box (5), the inner bottom of the installation box (5) is fixedly provided with a motor (6), the inner bottom of the installation box (5) is fixedly provided with a fixed frame body (9), the output end of the motor (6) penetrates through one side of the fixed frame body (9) and is fixedly connected with a first bevel gear (7), the outer surface of the first bevel gear (7) is meshed with a second bevel gear (8), the top of the fixed frame body (9) is connected with a rotating rod (10) in a penetrating manner, the bottom end of the rotating rod (10) is rotatably connected with the inner bottom of the installation box (5), the top end of the rotating rod (10) is fixedly, the top fixedly connected with fixed slide bar (13) of first carousel (11), spout (12) have been seted up at the top of install bin (5), the top fixedly connected with second carousel (14) of fixed slide bar (13).

2. A stem cell culture apparatus according to claim 1, wherein: the top fixed mounting of second carousel (14) has four fixed standing grooves (15), the inside of fixed standing groove (15) is provided with cultivates household utensils (16), one side through connection of fixed standing groove (15) has pull pole (17), the top fixedly connected with solid fixed splint (18) of pull pole (17), inner wall fixed connection telescopic link (19) of fixed standing groove (15), and the outside fixed connection of the other end of telescopic link (19) and solid fixed splint (18), the surface of telescopic link (19) is provided with reset spring (20).

3. A stem cell culture apparatus according to claim 1, wherein: dwang (10) and second bevel gear (8) fixed cup joint, the bottom of dwang (10) is provided with the pivot with the contact jaw of install bin (5).

4. A stem cell culture apparatus according to claim 1, wherein: the fixed sliding rod (13) is in sliding connection with the sliding groove (12), and the sliding groove (12) and the fixed sliding rod (13) are both in circular arrangement.

5. A stem cell culture apparatus according to claim 1, wherein: the outer surface of the rotating rod (10) and the contact end in the fixing frame body (9) are provided with bearings, and the second rotary table (14) and the first rotary table (11) are both in circular arrangement.

6. A stem cell culture apparatus according to claim 2, wherein: one side of the return spring (20) is fixedly connected with the inner wall of the fixed placing groove (15), and the other side of the return spring (20) is fixedly connected with the outer side of the fixed clamping plate (18).

7. A stem cell culture apparatus according to claim 2, wherein: the inner side of the fixed splint (18) is provided with a rubber pad which is arranged in an arc shape.