CN112342131B

CN112342131B - Experimental instrument for bacterial filtration efficiency

Info

Publication number: CN112342131B
Application number: CN202011065319.4A
Authority: CN
Inventors: 方元; 闫栋; 刘虎
Original assignee: Jinan Huanzheng Technology & Development Co ltd
Current assignee: Jinan Huanzheng Technology & Development Co ltd
Priority date: 2020-09-30
Filing date: 2020-09-30
Publication date: 2022-10-04
Anticipated expiration: 2040-09-30
Also published as: CN112342131A

Abstract

A bacterial filtration efficiency experimental instrument comprises a bacterial suspension conveying device, an aerosol chamber and a sampler which are communicated in sequence, wherein experimental materials are arranged between the aerosol chamber and the sampler, the bacterial suspension conveying device comprises a container, a high-pressure air pipe is arranged at the bottom of the container, the high-pressure air pipe extends into the container, a bacterial suspension conveying pipe is arranged at the top of the container and communicated with the aerosol chamber, and the bacterial suspension conveying pipe is arranged on the aerosol chamber and communicated with the aerosol chamber.

Description

Experimental instrument for bacterial filtration efficiency

Technical Field

The invention relates to the technical field of detection equipment, in particular to a bacterial filtration efficiency experimental instrument.

Background

The mask is a sanitary article, is generally worn at the position of the mouth and the nose for filtering air entering the mouth and the nose so as to achieve the effect of blocking harmful gas, smell, spray, virus and other substances, has a certain filtering effect on air entering the lung, and has a very good effect when being worn in the operation in the environment polluted by dust and the like when respiratory infectious diseases are prevalent.

Before the mask is put into use, the bacterial filtration efficiency of the mask needs to be experimentally measured, an experimental method for the bacterial filtration efficiency of the medical surgical mask is recorded in the national medical and pharmaceutical industry standard YY0469-2011, a continuous working machine is used for introducing bacterial suspension into a sprayer, then the sprayer is used for introducing the bacterial suspension into an aerosol chamber, a sampler communicated with the aerosol chamber is arranged at the bottom of the aerosol chamber, an experimental material is placed between the aerosol chamber and the sampler, and the bacterial filtration efficiency of the experimental material is obtained by measuring the bacterial content in the sampler and comparing the total bacterial content introduced into the aerosol chamber. However, the continuous working machine in the method needs additional power in the experimental process, not only is the structure complex, but also the total amount of bacteria conveyed by the continuous working machine is inconvenient to determine, and the final metering data is also inaccurate, so that the bacterial filtration efficiency value determined by the experimental material has a large error, and meanwhile, the particle size of the bacterial suspension entering the aerosol chamber cannot be adjusted according to the requirement of the material to be measured, and the finally measured data of the experiment can be influenced to a certain extent.

Disclosure of Invention

In order to solve the above problems, the present invention aims to provide a bacterial filtration efficiency experimental apparatus, in which a bacterial suspension conveying device connected to an aerosol chamber is installed on the aerosol chamber to replace the existing continuous working machine, so that the particle size of the bacterial suspension entering the aerosol chamber can be adjusted as required, the total amount of the conveyed bacteria can be conveniently measured, the measurement data is accurate, and the accuracy of the final measured bacterial filtration efficiency data can be ensured.

The technical scheme adopted by the invention for solving the technical problem is as follows: the invention relates to a bacterial filtration efficiency experimental instrument, which comprises a bacterial suspension conveying device, an aerosol chamber and a sampler which are communicated in sequence, wherein experimental materials are arranged between the aerosol chamber and the sampler, the bacterial suspension conveying device comprises a container, a high-pressure air pipe is arranged at the bottom of the container, the high-pressure air pipe extends into the container, a bacterial suspension conveying pipe is arranged at the top of the container and is communicated with the aerosol chamber, a ball is arranged between the bacterial suspension conveying pipe and the high-pressure air pipe, the diameter of the ball is the same as that of the high-pressure air pipe, the diameter of the ball is smaller than that of the bacterial suspension conveying pipe, a first through hole is formed in the bottom of the container, a second through hole is formed in the top of the container, a third through hole is formed in the side wall of the container, a bacterial suspension suction pipe communicated with the third through hole is arranged in the container, one end of the bacterial suspension suction pipe is fixedly connected with the inner wall of the container, the other end of the bacterial suspension is arranged between the high-pressure air pipe and the ball, the high-pressure air pipe blows the bacterial suspension into a safe biological suspension conveying cabinet body, and a biological suspension cabinet body are arranged in front of the aerosol chamber, and a biological suspension conveying cabinet body, the biological cabinet body is arranged in front of the high-pressure air pipe, and a biological suspension conveying cabinet body. And a convex edge is arranged in the bacterial suspension conveying pipe. The bacterial suspension conveying pipe comprises a straight pipe and an elbow, the straight pipe extends into the container, the elbow is communicated with the aerosol chamber, and the convex edge is positioned in the straight pipe. The high-pressure air pipe is fixedly provided with a vertically arranged connecting rod, and the connecting rod is provided with a ball. The bacterial suspension suction pipe is a conical pipe, and the diameter of one end, close to the high-pressure air pipe, of the bacterial suspension suction pipe is smaller than that of the end, communicated with the third through hole, of the bacterial suspension suction pipe. The top of the container is a conical surface, the bacterial suspension conveying pipe is positioned at the center of the conical surface, and the second through hole is formed in one side of the conical surface.

The invention has the positive effects that: according to the experimental instrument for the bacterial filtration efficiency, the existing continuous working machine is replaced by the bacterial suspension conveying device which is arranged on the aerosol chamber and is communicated with the aerosol chamber, the bacterial suspension conveying device is simple in structure, no additional power device is needed except for being connected with a high-pressure air source, compared with the original device, the total quantity of bacteria conveyed by the bacterial suspension conveying device is more convenient to measure, the measurement precision is accurate, the accuracy of the measured bacterial filtration efficiency value of the experimental material can be guaranteed, the particle size of the bacterial suspension entering the aerosol chamber can be changed by changing the connection mode of the through holes in the bacterial suspension conveying device according to the requirement of the material to be measured, the application range of the device is further expanded to a certain extent, and the accuracy of the finally measured data can be improved.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is an enlarged partial view of I of FIG. 1; (the dotted line in the figure indicates the flow direction of the bacterial suspension)

FIG. 3 is a schematic view of an alternative arrangement of the connection of the bacterial suspension delivery device to the aerosol chamber; (the dotted line in the figure indicates the flow direction of the bacterial suspension)

FIG. 4 is a schematic diagram of a prior art bacterial filtration efficiency test;

fig. 5 is a schematic view of the present invention in a biosafety cabinet.

In the figure 1, a bacterial suspension conveying device 2, an aerosol chamber 3, a sampler 4, an experimental material 5, a container 6, a high-pressure air pipe 7, a bacterial suspension conveying pipe 8, a ball 9, a first through hole 10, a second through hole 11, a third through hole 12, a bacterial suspension suction pipe 13, a connecting rod 14, a continuous working machine 15, a condenser 16, a flowmeter 17, a vacuum pump 18, a connecting hose 19, a cabinet 20, a bracket 21, a hinged member 23, a glass door 71, a straight pipe 72, an elbow 73, a convex edge 74 and an annular anti-backflow groove.

Detailed Description

The invention relates to a bacterial filtration efficiency experimental apparatus, as shown in figure 1, which comprises a bacterial suspension conveying device 1, an aerosol chamber 2 and a sampler 3 which are communicated in sequence, wherein an experimental material 4 is arranged between the aerosol chamber 2 and the sampler 3, the bacterial suspension sequentially passes through the aerosol chamber 2 and the experimental material 4 and finally enters the sampler 3, the number of bacteria blocking the experimental material 4 can be obtained by measuring the number of bacteria in the sampler 3 and the total number of bacteria conveyed into the aerosol chamber 2 and comparing the number of bacteria, and the bacterial filtration efficiency value of the experimental material 4 can be obtained.

Fig. 4 shows a prior art apparatus for performing a bacterial filtration efficiency experiment, wherein the aerosol chamber 2 is communicated with a continuous working machine 14, the continuous working machine 14 is used for inputting bacterial suspension into the aerosol chamber 2, in order to simulate the situation that the mask material to be tested filters bacteria when a person inhales, the bottom of the sampler 3 is further connected with a condenser 15 and a flow meter 16 in sequence through a conduit, and a vacuum pump 17 is further installed at the other end of the flow meter 16.

In order to greatly reduce the influence of the external environment on the experimental result when the experimental apparatus for the bacterial filtration efficiency is used for performing experiments, as shown in fig. 5, the bacterial suspension conveying device 1, the aerosol chamber 2 and the sampler 3 are all located in a biosafety cabinet, the biosafety cabinet comprises a cabinet body 19, a support 20 is installed at the bottom of the cabinet body 19, a cavity 21 is formed in the cabinet body 19, the cavity 21 is used for containing experimental instruments, a glass door 23 is installed on the cabinet body 19 at the front end of the cavity 21 through a hinge 22, the bacterial suspension conveying device 1 can be moved out of the cabinet body 19 when the glass door 23 is opened, so that an operator can conveniently add liquid to the bacterial suspension conveying device 1, adjust pipeline connection or perform weighing operation, and after the glass door 23 is closed, the bacterial suspension conveying device 1 can be pushed into the biosafety cabinet to ensure the accuracy of the experimental result.

As shown in fig. 2, the bacterial suspension conveying device 1 comprises a container 5, the container 5 is used for containing bacterial suspension, a high-pressure air pipe 6 is installed at the bottom of the container 5, the high-pressure air pipe 6 is externally connected with a high-pressure air source, the high-pressure air pipe 6 extends into the container 5, a bacterial suspension conveying pipe 7 is installed at the top of the container 5 corresponding to the position of the high-pressure air pipe 6, and the bacterial suspension conveying pipe 7 is communicated with the aerosol chamber 2 and is used for conveying the bacterial suspension in the container 5 into the aerosol chamber 2.

A round ball 8 is arranged between the bacterial suspension conveying pipe 7 and the high-pressure air pipe 6, wherein the diameter of the round ball 8 is the same as that of the high-pressure air pipe 6, and the diameter of the round ball 8 is smaller than that of the bacterial suspension conveying pipe 7. The bottom of the container 5 is further provided with a first through hole 9, the top of the container 5 is provided with a second through hole 10, the side wall of the container 5 is provided with a third through hole 11, a bacterial suspension suction pipe 12 communicated with the third through hole 11 is arranged inside the container 5, one end of the bacterial suspension suction pipe 12 is fixedly connected with the inner wall of the container 5, and the other end of the bacterial suspension suction pipe 12 is positioned between the high-pressure air pipe 6 and the ball 8. The end of the bacterial suspension suction pipe 12 can form negative pressure by the gas blown out from the high-pressure gas pipe 6, so that the bacterial suspension enters between the high-pressure gas pipe 6 and the round ball 8 from the bacterial suspension suction pipe 12, the bacterial suspension is blown into the bacterial suspension conveying pipe 7 by the high-pressure gas, and the third through hole 11 is communicated with the first through hole 9 or the second through hole 10.

When the particle size of the bacterial suspension to enter the aerosol chamber 2 is smaller, the bacterial suspension conveying device 1 is installed in a mode shown in fig. 1 and 2, one end of the high-pressure air pipe 6 is the bottommost part of the container 5, the first through hole 9 is communicated with the third through hole 11 through the connecting hose 18, the second through hole 10 is blocked after a certain amount of bacterial suspension is injected into the container 5, the liquid level of the bacterial suspension is lower than the height of the high-pressure air pipe 6 extending into the container 5, and the bacterial suspension is prevented from entering the high-pressure air pipe 6 when high-pressure air is not introduced. After the installation, high-pressure gas is introduced into the high-pressure gas pipe 6, the high-pressure gas is blown out from the bottom to the top in the direction shown in fig. 2, the high-speed flowing gas enables the end part of a bacterial suspension suction pipe 12 between the high-pressure gas pipe 6 and the round ball 8 to form negative pressure, bacterial suspension in the container 5 can enter the space between the high-pressure gas pipe 6 and the round ball 8 through the first through hole 9, the third through hole 11 and the bacterial suspension suction pipe 12 in sequence, and then rises under the driving of the high-pressure gas, most of the bacterial suspension can bypass the round ball 8 in a divergent shape to rise in the rising process due to the obstruction of the round ball 8, wherein the bacterial suspension with larger particle size can fall back to the bacterial suspension at the bottom of the container 5 under the action of gravity due to larger dead weight, the bacterial suspension with smaller particle size can enter the bacterial suspension conveying pipe 7 under the driving of the high-pressure gas, and the particle size of the bacterial suspension introduced into the aerosol chamber 2 is further ensured to be smaller. After the experiment is finished, the bacteria suspension conveying device 1 can be integrally disassembled to be weighed, so that the total amount of the bacteria suspension entering the aerosol chamber 2 can be calculated according to the difference between the front and the back of the experiment, the whole process is convenient to implement, and the data measurement is more accurate.

When the particle size of the bacterial suspension to enter the aerosol chamber 2 is large, the installation mode of the bacterial suspension conveying device 1 is as shown in fig. 3, one end of the bacterial suspension conveying pipe 7 is the bottommost part of the container 5, the second through hole 10 is communicated with the third through hole 11 through the connecting hose 18, the first through hole 9 is blocked after a certain amount of bacterial suspension is injected into the container 5, the liquid level of the bacterial suspension is lower than the height of the bacterial suspension conveying pipe 7 extending into the container 5, and the bacterial suspension is prevented from directly entering the aerosol chamber 2 when high-pressure gas is not introduced. After the installation, high-pressure gas is introduced into the high-pressure gas pipe 6, the high-pressure gas is blown out from top to bottom in the direction shown in fig. 3, the high-speed flowing gas enables the end part of the bacterial suspension suction pipe 12 between the high-pressure gas pipe 6 and the round ball 8 to form negative pressure, the bacterial suspension in the container 5 sequentially enters the space between the high-pressure gas pipe 6 and the round ball 8 through the second through hole 10, the third through hole 11 and the bacterial suspension suction pipe 12, and then falls into the bacterial suspension conveying pipe 7 under the drive of self weight and the high-pressure gas, and compared with the installation mode shown in fig. 2, the particle size of the bacterial suspension introduced into the aerosol chamber 2 is larger in the installation mode.

Further, in order to make the particle size of the bacterial suspension entering the aerosol chamber 2 smaller in the installation mode shown in fig. 2, the bacterial suspension delivery pipe 7 comprises a straight pipe 71 and an elbow 72, the straight pipe 71 extends into the container 5, the elbow 72 is communicated with the aerosol chamber 2, a convex edge 73 is further arranged inside the elbow 72 connected with the straight pipe 71, the elbow 72 can reduce the flow speed in the bacterial suspension delivery pipe 7, so that part of the bacterial suspension with larger particle size is attached to the pipe wall of the elbow 72, and after accumulation, the bacterial suspension with smaller particle size falls down along with self weight, the convex edge 73 can intercept the part with larger peripheral particle size in the rising bacterial suspension, and the bacterial suspension with smaller particle size is introduced into the aerosol chamber 2.

In the installation mode shown in fig. 3, the inside of the bacterial suspension conveying pipe 7 is also provided with the convex edge 73, and the annular anti-backflow groove 74 is formed between the outer periphery of the upper side of the convex edge 73 and the pipe wall, because the bacterial suspension in the installation mode shown in fig. 3 enters the bacterial suspension conveying pipe 7 under the double action of the blowing of high-pressure gas and gravity, the bacterial suspension with overlarge particle size can also directly enter the bacterial suspension conveying pipe 7, in order to avoid the situation, the annular anti-backflow groove 74 is formed between the outer periphery of the upper side of the convex edge 73 and the pipe wall, and the bacterial suspension with overlarge particle size can be blocked by the convex edge 73 and collected into the annular anti-backflow groove 74, so that the bacterial suspension meeting the requirement of the particle size enters the aerosol chamber 2. Wherein, each time the bacteria spraying is about 0.05mL, if 20% enters the anti-reflux groove, the bacteria liquid entering the annular anti-reflux groove 74 each time is 0.01mL, because the experiment needs to be finished within 3 hours after the bacteria liquid preparation, the total volume of the annular anti-reflux groove 74 is 0.4mL, the volume is enough to collect the bacteria suspension with overlarge particle size so as not to overflow the bacteria suspension, and the bacteria suspension with overlarge particle size is prevented from entering the aerosol chamber 2.

The first through hole 9 and the second through hole 10 can be blocked, the third through hole 11 is communicated with the continuous working machine 14 through the connecting hose 18, the continuous working machine 14 provides power to enable the bacterial suspension to enter the container 5, and then the bacterial suspension is sprayed into the aerosol chamber 2 through the high-pressure air pipe 6, so that the bacterial filtration efficiency experimental instrument can be suitable for various different testing modes. Work machine 14 may be a peristaltic pump.

Furthermore, a connecting rod 13 which is vertically arranged is fixedly installed on the high-pressure air pipe 6, and a round ball 8 is installed on the connecting rod 13.

Further, the bacterial suspension suction pipe 12 is a conical pipe, the diameter of the end of the bacterial suspension suction pipe 12 close to the high-pressure air pipe 6 is smaller than the diameter of the end communicated with the third through hole 11, wherein the diameter of the bacterial suspension suction pipe 12 close to the high-pressure air pipe 6 can be made small enough to ensure that the bacterial suspension is sucked from the container 5 to the space between the high-pressure air pipe 6 and the round ball 8 under the driving of high-pressure gas.

Furthermore, the top of the container 5 is a conical surface, the bacterial suspension delivery pipe 7 is positioned at the center of the conical surface, and the second through hole 10 is formed in one side of the conical surface. In the usage state shown in fig. 3, the conical container 5 can use a smaller amount of bacterial suspension while maintaining the same height of bacterial suspension, so as to ensure that the bacterial suspension can be continuously conveyed to the aerosol chamber 2, reduce or avoid the number of times of adding the bacterial suspension, and efficiently and accurately complete the bacterial filtration efficiency experiment.

The technical solution of the present invention is not limited to the scope of the embodiments of the present invention. The technical contents not described in detail in the present invention are all known techniques.

Claims

1. The utility model provides a bacterial filtration efficiency laboratory glassware which characterized in that: comprises a bacteria suspension conveying device (1), an aerosol chamber (2) and a sampler (3) which are communicated in sequence, wherein an experimental material (4) is arranged between the aerosol chamber (2) and the sampler (3), the bacteria suspension conveying device (1) comprises a container (5), a high-pressure air pipe (6) is arranged at the bottom of the container (5), the high-pressure air pipe (6) extends into the container (5) and corresponds to the position of the high-pressure air pipe (6), a bacteria suspension conveying pipe (7) is arranged at the top of the container (5), the bacteria suspension conveying pipe (7) is communicated with the aerosol chamber (2), a round ball (8) is further arranged between the bacteria suspension conveying pipe (7) and the high-pressure air pipe (6), the diameter of the round ball (8) is the same as that of the high-pressure air pipe (6), the diameter of the round ball (8) is smaller than that of the bacteria suspension conveying pipe (7), a first through hole (9) is further formed at the bottom of the container (5), a second through hole (10) is formed in the top of the container (5), a third through hole (11) is formed in the side wall of the container (5), a suction pipe (12) is fixedly connected with one end of the bacteria suspension (5) and is connected with a suction pipe (12) for fixing the bacteria suspension, the other end of the bacterial suspension suction pipe (12) is positioned between the high-pressure air pipe (6) and the round ball (8), negative pressure can be formed at the end part of the bacterial suspension suction pipe (12) by air blown by the high-pressure air pipe (6), further bacterial suspension enters the space between the high-pressure air pipe (6) and the round ball (8) from the bacterial suspension suction pipe (12), then the bacterial suspension is blown into the bacterial suspension conveying pipe (7) by high-pressure air, the third through hole (11) is communicated with the first through hole (9) or the second through hole (10), the bacterial suspension conveying device (1), the aerosol chamber (2) and the sampler (3) are all positioned in a biological safety cabinet, the biological safety cabinet comprises a cabinet body (19), a support (20) is installed at the bottom of the cabinet body (19), a cavity (21) is formed in the cabinet body (19), and a glass door (23) is installed on the cabinet body (19) at the front end of the cavity (21) through a hinge piece (22); a convex edge (73) is arranged in the bacterial suspension conveying pipe (7); the high-pressure air pipe (6) is fixedly provided with a vertically arranged connecting rod (13), and the connecting rod (13) is provided with a ball (8).

2. A bacteria filtration efficiency experimental apparatus according to claim 1, wherein: the bacteria suspension conveying pipe (7) comprises a straight pipe (71) and an elbow (72), the straight pipe (71) extends into the container (5), the elbow (72) is communicated with the aerosol chamber (2), and the convex edge (73) is positioned in the straight pipe (71).

3. A bacteria filtration efficiency experimental apparatus according to claim 1, wherein: the bacterial suspension suction pipe (12) is a conical pipe, and the diameter of one end, close to the high-pressure air pipe (6), of the bacterial suspension suction pipe (12) is smaller than that of the end, communicated with the third through hole (11).

4. A bacteria filtration efficiency experimental apparatus according to claim 1, wherein: the top of the container (5) is a conical surface, the bacterial suspension conveying pipe (7) is positioned at the center of the conical surface, and the second through hole (10) is formed in one side of the conical surface.