CN112326304B - Sample storage device for biotechnology and sample manufacturing method thereof - Google Patents
Sample storage device for biotechnology and sample manufacturing method thereof Download PDFInfo
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- CN112326304B CN112326304B CN202011125038.3A CN202011125038A CN112326304B CN 112326304 B CN112326304 B CN 112326304B CN 202011125038 A CN202011125038 A CN 202011125038A CN 112326304 B CN112326304 B CN 112326304B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
- G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
Abstract
The invention discloses a sample storage device for biotechnology and a sample manufacturing method thereof, wherein the sample storage device comprises a storage box body and is characterized in that: the novel slicing device is characterized in that a box cover is hinged to one side of the storage box body, a sample storage device is arranged inside the storage box body and comprises a first lifting grid plate and a second lifting grid plate, a central hinge is connected between the first lifting grid plate and the second lifting grid plate through a bearing, a knife holder is installed on the inner wall of the storage box body, a cutting knife is connected to the bottom of the knife holder, a first clamping portion is arranged on one side of the knife holder, a second clamping portion is arranged on the sliding portion, the first clamping portion and the second clamping portion are of a matched structure, a rack is arranged on the sliding groove, one end of the sliding portion is connected with a gear, the rack is meshed with the gear, one end of the sliding portion is connected with a motor, and the motor is connected with the inner wall of the storage box body in a sliding mode.
Description
Technical Field
The invention relates to the technical field of biological sampling, in particular to a sample storage device for biotechnology and a sample manufacturing method thereof.
Background
The field biological sampling comprises sampling plants such as wild mushrooms and sampling tissues, organs and excrement of wild animals, and the sampled samples need to be stored in a sample box for observation and other treatments. During the sampling research of stem plants, the plant is firstly sliced hierarchically, each tissue cross section of the plant is infiltrated by a fluorescent staining agent, and the growth 'annual rings' of the plant is observed by lighting so as to research the growth period of the plant.
The observation environment and the treatment measures are naturally lacked in the field, so that the experimenters need to carry the plants to an experimental site for research after the plants are subjected to freezing treatment, the process needs to carry freezing equipment and consume static force, and the frozen and then thawed plants lose part of the growth annual ring lines, so that the observation effect is poor. If slicing is performed in the field, a slicing device needs to be carried separately, and the slicing process is complicated.
In addition, the plant needs to make a specimen which is most suitable for the common shape, the process needs a big data fitting experiment of the volume shape of the stem plant, researchers need to manually measure the shape and the size of each cross section of the plant by using a laser scanner, and make a fitting curve, and the process needs to consume a large amount of manpower and inaccurate measuring results.
Therefore, it is necessary to design a sample storage device for biotechnology and a method of preparing a sample thereof, which are simple in slicing process and excellent in observation effect.
Disclosure of Invention
The present invention is directed to a sample storage device for biotechnology and a method for manufacturing a sample thereof, which solve the problems of the background art described above.
In order to solve the technical problems, the invention provides the following technical scheme: a biotechnological sample storage device comprising a storage cartridge, characterized in that: one side of the storage box body is hinged with a box cover, and a sample storage device is arranged inside the storage box body.
According to the technical scheme, the sample preservation device comprises a first lifting grid plate, a second lifting grid plate, a sliding portion and a sliding groove, a center hinge is connected between the first lifting grid plate and the second lifting grid plate through a bearing, a knife holder is installed on the inner wall of the storage box body, the bottom of the knife holder is connected with a cutting knife, one side of the knife holder is provided with a first clamping portion, a second clamping portion is arranged on the sliding portion, the first clamping portion and the second clamping portion are of a matched structure, a rack is arranged on the sliding groove, one end of the sliding portion is connected with a gear, the rack is meshed with the gear, one end of the sliding portion is connected with a motor, and the motor is connected with the inner wall of the storage box body in a sliding mode.
According to the technical scheme, the grids are embedded into one sides of the first lifting grid plate and the second lifting grid plate in a sliding mode, a penetrating dyeing portion is fixed to one end of each grid through a bolt, a positioning ring is installed on the other side of each grid, a sliding rod is arranged inside each grid in a sliding mode, electrode plates are correspondingly arranged outside each sliding rod, the positioning rings are conductive, a spring is installed on one side of each grid, and one end of each spring is fixed to each sliding rod.
According to the technical scheme, the inside of the sliding rod is provided with the pressing button in a sliding and inserting mode, the sliding rod is evenly provided with the clamping groove, the inner wall of the square is evenly provided with the clamping block in a sliding mode, the clamping block is matched with the clamping groove, the outer wall of the pressing button is wound with the elastic drawing wire, and the elastic drawing wire is connected with the clamping block.
According to the technical scheme, the inner wall of the square is provided with the irradiation lamp, one end of the square is connected with the installation sleeve in a screwing mode through threads, the convex lens is installed inside the installation sleeve, and the moon-shaped lens is fixedly arranged on one side of the convex lens through bonding.
According to the technical scheme, one end of the square is connected with a permeable dyeing part, a permeable groove is formed in the inner wall of the permeable dyeing part, the bottom of the permeable groove is connected with a soaking groove in a penetrating mode, the permeable groove is adjacent to the convex lens, one end of the permeable groove is connected with a permeable liquid pipe, and one end of the permeable liquid pipe is connected with a permeable liquid input end in a penetrating mode.
According to the technical scheme, one side of the square is provided with the light splitting assembly, the light splitting assembly comprises a light splitting sleeve, the inner wall of the light splitting sleeve is fixedly provided with the phase delay piece and the Wollaston lens through threaded screwing respectively, and the opening of the light splitting sleeve is provided with the birefringent wedge-shaped prism.
According to the technical scheme, the splicing part is formed in one side of the first lifting grid plate, the sodium azide bag is arranged on the inner wall of the splicing part, the extrusion part is arranged on one side of the first lifting grid plate, and the air outlet flow channel is formed in the inner wall of the first lifting grid plate.
A method for preparing a specimen in a specimen storage device for biotechnology, comprising: comprises that
Firstly, flatly placing the stem plants on a first layer of lifting grid plate, taking the lower left corner as an original point, the bottom edge as an X axis and the left side edge as a Y axis, recording the X-axis minimum value and the X-axis maximum value coordinate corresponding to the Y-axis coordinate according to the resistance value change brought by the pressed grids by using the value corresponding to Y, Y, Y, Y … … YN from small to large of the Y axis, and connecting adjacent coordinates to obtain the contour line of the first layer;
step two, making contour lines on each layer of the layered and sliced stem plants, taking the height of each layer of slices as a Z axis, and connecting the corresponding positions of the Z axis of each layer of contour lines from bottom to top to obtain a three-dimensional contour image of the stem plants;
step three, importing the plurality of three-dimensional contour images into a computer, and fitting by using fitting software to obtain a most universal plant virtual contour map;
step four, guiding the virtual contour map into the sample storage device, splicing the lifting grid plates where the grids are located into a whole, and taking down the grids where the contour is located according to the coordinates of the contour map to form a closed contour space;
and fifthly, smashing the cut stem plants into a slag shape, pouring the mixed liquid of the gel, the preservative and the slag stem plants into a lifting grid plate, standing for a period of time, and detaching the lifting partition plates one by one after the mixed liquid is solidified to obtain stem plant sample specimens.
Compared with the prior art, the invention has the following beneficial effects: according to the invention, through arranging the components such as the lifting grid plate, the stem plants can be automatically sliced and dyed for observation of inspectors, the slicing is not required to be carried out manually, meanwhile, a fitting curve of the plant volume can be manufactured according to the number and the distribution mode of the lowered grids, a handheld scanner is not required to scan the plants, and the measurement process is convenient.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of the sample retention device of the present invention;
FIG. 3 is a schematic view of the internal cross-sectional structure of the storage case of the present invention;
FIG. 4 is a schematic view of a grid and slide bar installation of the present invention;
FIG. 5 is a schematic diagram of the internal structure of a grid according to the present invention;
in the figure: 1. a storage box body; 11. a box cover; 2. a sample retention device; 3. a cutting knife; 21. lifting the grid plate I; 22. a second lifting grid plate; 23. a central hinge; 24. grid; 241. a penetrating dyeing part; 2411. a permeation groove; 2412. soaking the tank; 242. a positioning ring; 243. pressing the button; 244. a slide bar; 245. a spring; 246. an electrode sheet; 247. illuminating a lamp; 248. a permeate tube; 2481. a permeate input; 249. installing a sleeve; 2491. a convex lens; 2492. a moon-shaped lens; 25. a sliding part; 31. a tool apron; 4. a clamp; 41. a splint; 5. a chute.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-5, the present invention provides the following technical solutions: a sample storage device for biotechnology, comprising a storage case 1, characterized in that: one side of the storage box body 1 is hinged with a box cover 11, a sample storage device 2 is arranged in the storage box body 1, when sampling is carried out, firstly, a taken stem plant sample is sent into the storage box body 1, and the sample is stored by the sample storage device 2;
the sample storage device 2 comprises a first lifting grid plate 21, a second lifting grid plate 22, a sliding part 25 and a sliding groove 5, wherein a central hinge 23 is connected between the first lifting grid plate 21 and the second lifting grid plate 22 through a bearing, a tool apron 31 is installed on the inner wall of the storage box body 1, a cutting tool 3 is connected to the bottom of the tool apron 31, a first clamping part is arranged on one side of the tool apron 31, a second clamping part is arranged on the sliding part 25, the first clamping part and the second clamping part are of a matched structure, a rack is arranged on the sliding groove 5, one end of the sliding part 25 is connected with a gear, the rack is meshed with the gear, one end of the sliding part 25 is connected with a motor, the motor is connected with the inner wall of the storage box body 1 in a sliding manner, when a sample is sliced, the motor is started firstly, an output shaft of the motor drives the gear to rotate, so that the tool apron 31 is driven to move on the sliding groove 5, the positions of the first lifting grid plate 21 and the second lifting grid plate 22 are switched, therefore, slices with different horizontal heights can be accommodated, when the slices slide to the position below the cutting knife 3, the torque of the knife holder 31 is driven to slice the stem plants, and the first lifting grid plate 21 and the second lifting grid plate 22 are hinged with each other, so that the slices can be folded to save space when not being mounted;
a grid 24 is embedded into one side of each of the first lifting grid plate 21 and the second lifting grid plate 22 in a sliding manner, one end of the grid 24 is fixed with a permeable dyeing part 241 through a bolt, the other side of the grid 24 is provided with a positioning ring 242, a sliding rod 244 is arranged inside the grid 24 in a sliding manner, an electrode plate 246 is correspondingly arranged outside the sliding rod 244, the positioning ring 242 has conductivity, one side of the grid 24 is provided with a spring 245, one end of the spring 245 is fixed with the sliding rod 244, when the squares 24 are pressed inwardly by the force of the plant slices, the squares 24 slide inwardly, thereby compressing the springs 245, then the positioning ring 242 is pushed to move on the sliding rod 244, the contact position of the positioning ring 242 and the electrode sheet 246 is equivalent to the principle of a slide rheostat, the square 24 is determined to enter the first lifting grid plate 21 and the second lifting grid plate 22 by using the change of the resistance, and therefore the signal is converted into an electric signal to prepare for a subsequent fitting image;
the inside of the sliding rod 244 is provided with a pressing button 243 in a sliding and inserting mode, the sliding rod 244 is uniformly provided with clamping grooves, the inner wall of each square 24 is uniformly provided with a clamping block in a sliding mode, the clamping block is matched with the clamping grooves, the outer wall of the pressing button 243 is wound with an elastic traction wire, the elastic traction wire is connected with the clamping block, when plant slices are pressed on the first lifting grid plate 21 and the second lifting grid plate 22, the square 24 is pressed inwards by the acting force of the elastic traction wire, the square 24 is positioned by being pressed by the clamping block and cannot be restored to the original state along with the taking out of a sample, when the original state needs to be restored, the pressing button 243 is pressed downwards, so that the elastic traction wire pulls the clamping block back to the inside of the sliding rod 244, the spring 245 is restored to the original state, and the shape memory and the quick restoration of the position of the clamping block are facilitated;
the inner wall of the square grid 24 is provided with a radiation lamp 247, one end of the square grid 24 is connected with a mounting sleeve 249 in a screwing mode through threads, a convex lens 2491 is mounted inside the mounting sleeve 249, a moon-shaped lens 2492 is fixedly bonded on one side of the convex lens 2491, after slicing is finished, the radiation lamp 247 is started, the convex lens 2491 and the moon-shaped lens 2492 are used for collimating divergent laser output by the radiation lamp 247 by using a refraction principle, and laser beams are changed into parallel beams after passing through the collimation lens, so that human eyes can observe the annual rings of plants conveniently;
one end of the square 24 is connected with a permeable dyeing part 241, the inner wall of the permeable dyeing part 241 is provided with a permeable groove 2411, the bottom of the permeable groove 2411 is connected with a soaking groove 2412 in a penetrating way, the permeable groove 2411 is adjacent to the convex lens 2491, one end of the permeable groove 2411 is connected with a permeable liquid pipe 248, one end of the permeable liquid pipe 248 is connected with a permeable liquid input end 2481 in a penetrating way, when the permeable liquid is injected, as the temperature of the convex lens 2491 and the lunar lens 2492 is easily raised by direct strong light, firstly the permeable liquid is introduced into the permeable groove 2411 to play a role in cooling the convex lens 2491 and the lunar lens 2492, and then the cut piece is oozed from the soaking groove 2412 to be dyed so as to be convenient for observation;
a light splitting assembly is arranged on one side of the grid 24 and comprises a light splitting sleeve, a phase delay plate and a Wollaston lens are fixedly screwed on the inner wall of the light splitting sleeve through threads respectively, a birefringent wedge-shaped prism is arranged at an opening of the light splitting sleeve, laser emitted from the inside of the irradiation lamp 247 has four different wavelengths, the polarization state of the laser with the first wavelength lambda 1 is kept unchanged through the phase delay plate, the polarization of the second lambda 2 rotates 90 degrees, the polarization of the third lambda 3 rotates 180 degrees, the polarization of the fourth lambda 4 rotates 270 degrees, the Wollaston lens separates light beams into four beams according to angles, then the four beams are converted into parallel light through the birefringent wedge-shaped prism, and the irradiation of the irradiation lamp 247 can be realized, so that energy and part quantity are saved;
the splicing part is arranged on one side of the first lifting grid plate 21, the sodium azide bags are arranged on the inner wall of the splicing part, the extrusion part is arranged on one side of the first lifting grid plate 21, the air outlet flow channel is arranged on the inner wall of the first lifting grid plate 21, when the first lifting grid plates 21 are extruded together, the sodium azide bags are broken by the extrusion part, the sodium azide is decomposed to generate a large amount of nitrogen, the nitrogen is discharged through the air outlet flow channel, the slices on the first lifting grid plate 21 are blown away through a large amount of air flow, the slices can be conveniently and quickly cleaned and then reused, the trouble of manual cleaning is avoided, and when the slices are reused, only new sodium azide bags need to be placed into the splicing part;
a method for preparing a specimen in a specimen storage device for biotechnology, comprising: comprises that
Firstly, flatly placing stem plants on a first layer of lifting grid plate, taking the lower left corner as an original point, the bottom edge as an X axis and the left side edge as a Y axis, taking values corresponding to Y1, Y2, Y3 and Y4 … … YN of the Y axis from small to large, recording an X axis minimum value and an X axis maximum value coordinate corresponding to the Y axis coordinate according to resistance value change brought by pressed grids, connecting adjacent coordinates to obtain a contour line of the first layer, and converting sunk grids into coordinate parameters so as to realize standardized contour tracing;
step two, making contour lines on each layer of the layered and sliced stem plants, taking the height of each layer of slices as a Z axis, and connecting the corresponding positions of the Z axis of each layer of contour lines from bottom to top to obtain a three-dimensional contour image of the stem plants;
step three, importing the plurality of three-dimensional contour images into a computer, and fitting by using fitting software to obtain a most universal plant virtual contour map;
step four, guiding the virtual contour map into the sample storage device, splicing the lifting grid plates where the grids are located into a whole, and taking down the grids where the contour is located according to the coordinates of the contour map to form a closed contour space;
and fifthly, smashing the cut stem plants into a slag shape, pouring mixed liquid of the gel, the preservative and the slag stem plants into a lifting grid plate, standing for a period of time, and detaching lifting partition plates one by one after the mixed liquid is solidified to obtain stem plant sample specimens.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (8)
1. A sample storage device for biotechnology, includes storage box body (1), its characterized in that: a box cover (11) is hinged to one side of the storage box body (1), and a sample storage device (2) is arranged inside the storage box body (1);
the sample storage device (2) comprises a first lifting grid plate (21), a second lifting grid plate (22), a sliding part (25) and a sliding chute (5), a central hinge (23) is connected between the first lifting grid plate (21) and the second lifting grid plate (22) through a bearing, a cutter holder (31) is arranged on the inner wall of the storage box body (1), the bottom of the cutter holder (31) is connected with a cutting-off cutter (3), a first clamping part is arranged on one side of the cutter holder (31), a second clamping part is arranged on the sliding part (25), the first clamping part and the second clamping part are of a matching structure, a rack is arranged on the chute (5), one end of the sliding part (25) is connected with a gear, the rack is meshed with the gear, one end of the sliding part (25) is connected with a motor, and the motor is connected with the inner wall of the storage box body (1) in a sliding manner.
2. A biotechnological sample storage device according to claim 1 characterised by: the utility model discloses a lift check, including lift check board (21) and lift check board two (22), the equal slip embedding in one side of check (24), the one end of check (24) is fixed with infiltration dyeing portion (241) through the bolt, holding ring (242) are installed to the opposite side of check (24), the inside slip of check (24) is provided with slide bar (244), the outside correspondence of slide bar (244) is provided with electrode piece (246), holding ring (242) have electric conductivity, spring (245) are installed to one side of check (24), the one end and the slide bar (244) of spring (245) are fixed mutually.
3. A biotechnological sample storage device according to claim 2 characterised by: the inner part of the sliding rod (244) is provided with a pressing button (243) in a sliding and inserting mode, the sliding rod (244) is evenly provided with clamping grooves, the inner wall of the square (24) is evenly provided with clamping blocks in a sliding mode, the clamping blocks are matched with the clamping grooves, the outer wall of the pressing button (243) is wound with an elastic traction wire, and the elastic traction wire is connected with the clamping blocks.
4. A biotechnological sample storage device according to claim 3 characterised by: the inner wall of square (24) is provided with lamp (247), the one end of square (24) is connected with installation cover (249) through the screw thread closure soon, the internally mounted of installation cover (249) has convex lens (2491), one side of convex lens (2491) is fixed with moon-shaped lens (2492) through the bonding.
5. A biotechnological sample storage device according to claim 4 characterised by: one end of the square (24) is connected with a permeable dyeing part (241), a permeable groove (2411) is formed in the inner wall of the permeable dyeing part (241), a soaking groove (2412) is connected to the bottom of the permeable groove (2411) in a penetrating mode, the permeable groove (2411) is adjacent to the convex lens (2491), one end of the permeable groove (2411) is connected with a permeable liquid pipe (248), and one end of the permeable liquid pipe (248) is connected with a permeable liquid input end (2481) in a penetrating mode.
6. A biotechnological sample storage device according to claim 5 characterised by: one side of square (24) is provided with the beam splitting subassembly, the beam splitting subassembly includes the beam splitting cover, the inner wall of beam splitting cover is fixed with phase delay piece and Wollaston lens through the screw thread is closed soon respectively, the opening part of beam splitting cover is provided with birefringent wedge prism.
7. A biotechnological sample storage device according to claim 6 characterised by: the air-out flow passage is formed in the inner wall of the first lifting grid plate (21).
8. A biotechnological sample storage device according to any one of claims 1-7 characterised by: comprises that
Firstly, flatly placing the stem plants on a first layer of lifting grid plate, taking the lower left corner as an original point, the bottom edge as an X axis and the left side edge as a Y axis, taking values corresponding to Y1, Y2, Y3 and Y4 … … YN of the Y axis from small to large, recording an X-axis minimum value and an X-axis maximum value coordinate corresponding to the Y coordinate according to resistance value change brought by a pressed grid, and connecting adjacent coordinates to obtain a contour line of the first layer;
step two, making contour lines on each layer of the layered and sliced stem plants, taking the height of each layer of slices as a Z axis, and connecting the corresponding positions of the Z axis of each layer of contour lines from bottom to top to obtain a three-dimensional contour image of the stem plants;
step three, importing the plurality of three-dimensional contour images into a computer, and fitting by using fitting software to obtain a most universal plant virtual contour map;
step four, guiding the virtual contour map into the sample storage device, splicing the lifting grid plates where the grids are located into a whole, and taking down the grids where the contour is located according to the coordinates of the contour map to form a closed contour space;
and fifthly, smashing the cut stem plants into a slag shape, pouring the mixed liquid of the gel, the preservative and the slag stem plants into a lifting grid plate, standing for a period of time, and detaching the lifting partition plates one by one after the mixed liquid is solidified to obtain stem plant sample specimens.
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| CN115384908A (en) * | 2022-10-10 | 2022-11-25 | 江苏浦贝智能科技有限公司 | Storage box for precision mold production workshop |
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| JP2010054482A (en) * | 2008-08-29 | 2010-03-11 | Seiko Instruments Inc | Automatic slicing device |
| CN102004305A (en) * | 2009-08-31 | 2011-04-06 | 索尼公司 | Tissue-slice image acquirement and display apparatus, tissue-slice image acquirement and display method, and tissue-slice image acquirement and display program |
| CN103038623A (en) * | 2010-07-29 | 2013-04-10 | 樱花精机株式会社 | Method for forming tissue pieces for tissue array, and device for forming tissue pieces for tissue array |
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| JP2005098702A (en) * | 1999-10-07 | 2005-04-14 | Seitai Kagaku Kenkyusho:Kk | Biological tissue sample preparation method and apparatus |
| US8840608B2 (en) * | 2002-03-15 | 2014-09-23 | The General Hospital Corporation | Methods and devices for selective disruption of fatty tissue by controlled cooling |
| CN101375682B (en) * | 2008-10-03 | 2011-08-24 | 罗荣 | Biological tissue slice supporting agent and method for carrying quick slice in surgery using the same |
| CN103185673A (en) * | 2011-12-30 | 2013-07-03 | 李朝林 | Integrative box type multifunctional histotome |
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| JP2010054482A (en) * | 2008-08-29 | 2010-03-11 | Seiko Instruments Inc | Automatic slicing device |
| CN102004305A (en) * | 2009-08-31 | 2011-04-06 | 索尼公司 | Tissue-slice image acquirement and display apparatus, tissue-slice image acquirement and display method, and tissue-slice image acquirement and display program |
| CN103038623A (en) * | 2010-07-29 | 2013-04-10 | 樱花精机株式会社 | Method for forming tissue pieces for tissue array, and device for forming tissue pieces for tissue array |
| CN108872174A (en) * | 2018-07-05 | 2018-11-23 | 南京林业大学 | A kind of method of rapid examination green bamboo blastular structure |
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