CN112293352B - Detection device for biological antenna sensing mechanism research - Google Patents
Detection device for biological antenna sensing mechanism research Download PDFInfo
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- CN112293352B CN112293352B CN202011244173.XA CN202011244173A CN112293352B CN 112293352 B CN112293352 B CN 112293352B CN 202011244173 A CN202011244173 A CN 202011244173A CN 112293352 B CN112293352 B CN 112293352B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention belongs to the technical field of biological behavior detection, and particularly relates to a detection device for researching a biological touch perception mechanism, which comprises an induction switching module, a biological touch experiment automatic alternation module and an antenna position alignment module; wherein: the induction switching module can realize the automatic switching of different induction modes in the experimental process through the matching action of the automatic alternation and the automatic stretching of the inducer; the tentacle position alignment module mainly comprises an image acquisition processing device and an angle adjusting device, the position deviation of the base line of the biological tentacle is obtained through calculation of the image acquisition processing device, and the deviation correction is realized through the angle adjusting device, so that the direction of the base line of the biological tentacle is kept consistent with the reference line; the automatic alternation module for the biological touch test realizes the automatic alternation of the test organisms in the standard environment; therefore, the realization of the device can be used for establishing biological tactile mechanism research and improving the standardization degree of tactile experiments.
Description
Technical Field
The invention belongs to the technical field of biological behavior detection, and particularly relates to a detection device for researching a biological antenna sensing mechanism.
Background
The biological behavior detection device is a bridge connecting life science and information science, and is an essential advanced automatic detection and observation device for biological behavior development. In recent years, modern biological behavior detection technology is gradually mature, and has qualitative breakthrough in detection objects, detection methods, monitoring parameters, experimental accuracy, efficiency and cost, and is widely applied to various research fields such as medicine, molecular biology, physiology, neuroscience and the like.
For the detection of biological antenna sensing, a common method is to manually contact the antenna of a living being with a reagent, but inevitably, human factors exist in the detection process, and the experimental result is influenced. In order to improve the accuracy of biological antenna sensing detection and the experimental research efficiency, an automatic detection device for biological antenna sensing mechanism research is provided.
In future research, the problems of flexibility of sensor selection and sensitivity of sensors, aging of biological behavior detection devices, service life of signal detectors, stability of signal response, convenience of detection devices and the like still need to be solved. The research and development of an automatic experimental device for realizing biological behavior detection play an important role in finding the rule in an ecological system, utilizing and protecting biological resources and enabling people and the environment to be harmonious, and finally, the rules and the development serve the human beings in turn.
Disclosure of Invention
The invention aims to provide a detection device for researching a biological antenna sensing mechanism so as to solve the defects or problems in the background technology.
In order to achieve the above object, an embodiment of the present invention provides a detection apparatus for biological antenna sensing mechanism research, which is characterized in that the detection apparatus comprises an induction switching module, a biological tactile sensation experiment automatic rotation module, and an antenna position alignment module;
the induction switching module comprises an automatic inducer switching device and an automatic inducer telescoping device; the automatic inducer switching device comprises a first rotating platform and a first rotating disc arranged on the first rotating platform, wherein m first round holes for placing reagent tubes are uniformly formed in the first rotating disc; the automatic inducer telescoping device comprises a gear, a rack and an electromagnetic magnet, the gear is meshed with the rack and is fixedly connected with the rack, the electromagnetic magnet consists of an iron core, a coil and an armature which is detachably connected to the iron core, the armature is sleeved on a reagent tube, the iron core generates electromagnetic attraction and attracts the armature under the condition that the coil is electrified, the iron core loses magnetic force and is disconnected from the armature to adsorb under the condition that the coil is powered off, the gear is driven to rotate by a first motor, the gear is meshed with the rack to drive the electromagnetic magnet, and the iron core of the electromagnetic magnet adsorbs the armature to drive the reagent tube to stretch and move under the condition that the coil is powered on;
the biological touch sensation experiment automatic alternation module adopts an experiment biological automatic alternation device in a standard environment; the experimental organism automatic conversion device comprises a second rotating platform and a second rotating disc, wherein a plurality of second round holes for placing the biological fixing devices are uniformly formed in the second rotating disc, a biological fixing device is sleeved in each second round hole, and the biological fixing devices are used for placing experimental organisms;
the antenna position alignment module comprises an image acquisition processing device and an angle adjusting device; the image acquisition and processing device is arranged above the insect training standard environment and comprises an industrial camera and image acquisition software corresponding to the industrial camera, the industrial camera realizes the photographing of the tentacle positions of bees, the image acquisition software realizes the processing and analysis of the acquired images and calculates the angle deviation between the central lines of the two tentacles of the bees and the reference line of the biological fixing device; the angle adjusting device comprises a fine adjusting device, the fine adjusting device comprises a switching device and a power device which are fixed below the biological fixing device, the power device comprises a linear rotating motor and a rotary adjusting workpiece, the linear rotating motor is used for realizing axial linear motion and rotary motion, the linear rotating motor is fixedly connected with the rotary adjusting workpiece, and an output shaft of the linear rotating motor drives the rotary adjusting workpiece to realize and rotate along the linear directionSwitching deviceThe output shaft of the linear rotating motor starts to rotate after the rotation adjusting workpiece is in butt joint with the switching device, the output shaft of the linear rotating motor adjusts the calculated angle deviation between the central lines of the two tentacles of the bees and the reference line of the biological fixing device in the rotating process, the linear rotating motor drives the switching device to adjust the bees to the correct position convenient for contacting with the reagent, and the switching device is used for connecting the biological fixing device and the power device.
Furthermore, the rotation adjusting workpiece is fixedly connected to an output shaft of the linear rotating motor through a connecting piece, and a connecting lug is arranged on the rotation adjusting workpiece.
Furthermore, the biological fixing device is provided with a containing cavity for containing the experimental organism, and the containing cavity is a through hole.
Preferably, the upper end of the adapter device is fixedly connected below the accommodating cavity, and a connecting groove matched with the connecting projection is formed below the adapter device.
Furthermore, the number of the first round holes is m, and the number of the second round holes is n.
Furthermore, the first rotating platform and the second rotating platform are both provided with electric indexing disc rotating platforms, and a first round hole formed in the first rotating disc and a second round hole formed in the second rotating disc are both in circumferential arrays.
Furthermore, the detection device for the biological antenna perception mechanism research further comprises a moving device, wherein the moving device comprises a one-dimensional moving device and a two-dimensional moving device, the one-dimensional moving device is used for moving a first rotating platform and the two-dimensional moving device is used for moving a second rotating platform, the one-dimensional moving device is connected with the first rotating platform of the inducer automatic wheel device and drives the first rotating platform to move along the Z-axis direction, the two-dimensional moving device is connected with the second rotating platform of the experimental biological automatic wheel device and drives the second rotating platform to move along the X-axis direction and the Y-axis direction.
Specifically, the one-dimensional moving device selects a vertical ball screw sliding table mechanism, and the two-dimensional moving device is formed by superposing two horizontal ball screw sliding table mechanisms.
Specifically, the vertical ball screw sliding table mechanism and the two horizontal ball screw sliding table mechanisms are driven by the second motor.
Furthermore, a liquid reagent is arranged in the reagent tube, the liquid reagent is electrically connected with the grounding end of the signal acquisition device through a lead, a biological (bee) is electrically connected with a certain input end of a data acquisition card in the signal acquisition device, a biological (bee) antenna and the reagent can generate a bioelectricity signal when contacting, and the data acquisition card acquires the bioelectricity signal through the certain input end of the signal acquisition device and transmits the acquired bioelectricity signal to a computer.
The technical scheme of the invention has the following beneficial effects:
(1) the invention relates to a detection device for researching a biological antenna sensing mechanism, which comprises an induction switching module, a biological tactile experiment automatic alternation module and an antenna position alignment module; wherein: the induction switching module can realize the automatic switching of different induction modes in the experimental process through the matching action of the automatic alternation and the automatic stretching of the inducer; the tentacle position alignment module mainly comprises an image acquisition processing device and an angle adjusting device, the position deviation of the base line of the biological tentacle is obtained through calculation of the image acquisition processing device, and the deviation correction is realized through the angle adjusting device, so that the direction of the base line of the biological tentacle is kept consistent with the reference line; meanwhile, the automatic alternation module for the biological touch experiment realizes the automatic alternation of the experimental organisms in the standard environment; therefore, the realization of the device can be used for establishing biological tactile mechanism research and improving the standardization degree of tactile experiments.
(2) The detection device for researching the biological antenna sensing mechanism realizes that a plurality of experimental organisms perform experiments on the same reagent by the arrangement of the induction switching module and the biological touch experiment automatic alternation module, can realize research experiments on a plurality of reagents by a single experimental organism, and has wide applicability and convenient application.
(3) The invention realizes the electrophysiological signal acquisition during the contact of the biological antennae and the behavior image acquisition during the contact process, thereby researching and analyzing the tactile-behavior mechanism of the organisms. The invention adopts the image acquisition and processing method to calculate and judge the position of the biological antenna, and has higher precision. In the process of the antenna perception detection, the living beings actively contact the reagent by using the own touch organs, human factors are eliminated, and the biological behavior detection device can simultaneously acquire biological electric signals and record the behavior tracks of the touch organs.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic structural diagram of an automatic rotation module for a biological tactile assay according to the present invention;
FIG. 3 is a schematic structural diagram of an induced switching module according to the present invention;
FIG. 4 is a view showing the connection between the adapter device and the bio-immobilization device according to the present invention;
FIG. 5 is a schematic view of the adapter of the present invention;
FIG. 6 is a schematic view of the structure of the rotation regulating member according to the present invention;
fig. 7 is a schematic diagram of a biological (bee) position deviation image according to the present invention.
Description of reference numerals: 100. a one-dimensional moving device; 200. an induction switching module; 201. a first rotating platform; 202. a first turntable; 203. a reagent tube; 204. an armature; 205. a rack; 206. a gear; 207. electromagnetic attracting iron; 208. a first motor; 209. a fixed block; 210. a sliding screw platform; 300. the automatic alternation module for the biological tactile test; 301. a second turntable; 302. a second rotary platform; 303. a biological fixation device; 304. a rolling bearing; 305. a sliding screw platform; 400. a two-dimensional moving device; 410. a horizontal ball screw sliding table mechanism; 420. a horizontal ball screw sliding table mechanism; 411. a slider; 412. a screw rod; 413. a coupling; 414. a second motor; 415. a bearing; 416. a base; 500. an angle adjusting device; 510. a linear rotating electric machine; 520. rotating the adjusting workpiece; 521. a connection bump; 530. a switching device; 531. connecting grooves; 540. a connecting member.
Detailed Description
In order to make the technical problems, technical solutions and advantages of the present invention more apparent, the following detailed description is given with reference to the accompanying drawings and specific embodiments.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", "front", "rear", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
As shown in fig. 1, a detection apparatus for biological antenna sensing mechanism research includes an induction switching module 200, a one-dimensional moving apparatus 100, a biological tactile sensation experiment automatic rotation module 300, a two-dimensional moving apparatus 400, and an antenna position alignment module.
Wherein, the inducing switching module 200 comprises an inducer automatic rotation device and an inducer automatic expansion device; the automatic inducer switching device comprises a first rotating platform 201 and a first rotating disc 202 arranged on the first rotating platform, wherein m first round holes for placing reagent tubes 203 are uniformly formed in the first rotating disc 202, and the tail ends of the reagent tubes 203 are sleeved with armatures 204; the liquid in the reagent tube 203 is connected with a grounding end of a signal acquisition device, the automatic inducer telescoping device comprises a gear 206, a rack 205 and an electromagnetic magnet 207, the gear 206 is meshed with the rack 205, the electromagnetic magnet 207 is fixedly connected with the rack 205, the gear 206 is driven to rotate by a first motor 208, the gear 206 drives the electromagnetic magnet 207 by the rack 205, the electromagnetic magnet 207 comprises a coil, an iron core and an armature 204 which is detachably connected to the iron core, and the iron core 204 of the electromagnetic magnet 207 adsorbs the armature 204 to drive the reagent tube 203 to move telescopically under the condition that the coil is electrified; when the coil of the iron core of the electromagnet 207 is powered off, the magnetic force of the iron core disappears, and the iron core is disconnected from the armature 204 for adsorption; in the embodiment of the present invention, a one-dimensional moving device 100 for adjusting the height of the first rotating platform 201 is disposed on one side of the first rotating platform 201, the one-dimensional moving device 100 is connected to the first rotating platform, and the one-dimensional moving device 100 drives the first rotating platform 201 to move along the Z-axis direction; the whole induction switching module 200 is driven to move up and down by controlling the one-dimensional moving device 100, so that the lowest position of the circle center of one first circular hole on the first rotary disc 202 and the axis position of the electromagnetic magnet 207 are on the same horizontal line; the automation degree is high; specifically, the one-dimensional moving device 100 selects a vertical ball screw sliding table mechanism.
As shown in fig. 3, the automatic inducer switching device, specifically, in this embodiment, the first rotary table 202 and the electric indexing disc rotary table (first rotary table 201) are coaxially assembled through the sliding screw platform 210, the diameter of the first rotary table 202 is 300mm, the height of the first rotary table is 30mm, m first circular holes are arranged in a circumferential array at a position 132mm away from the center of the first rotary table 202, the diameter of the m first circular holes is 5mm, the number of the first circular holes and the number of the second circular holes can be determined by the number of reagent tubes 203 required in an experiment and the number of experimental creatures, and the invention is not limited thereto.
In a further embodiment of the invention, the first circular holes of the first carousel 202 are each inserted with a reagent vessel 203, the reagent vessel 203 being 16mm long and 4.2mm in diameter. The reagent tube 203 extends through the first turntable 202 and is connected at the end of the reagent tube 203 to an armature 204 with an inner diameter of 8mm and an outer diameter of 12 mm.
As shown in fig. 3, the automatic telescopic device for an inducer is specifically, in this embodiment, the armature 204 is fixed at the end of the reagent tube 203, the electromagnetic iron 207 is fixedly connected with the end of the rack 205, and may be fixedly connected by bolts or the like, and the axial center position of the electromagnetic iron 207 and the lowest position of the center of one of the first circular holes on the first rotating disk 202 are on the same horizontal line. The gear 206 is connected with a first motor 208 through a coupling, and the first motor 208 drives the gear 206 to rotate. In the present embodiment, the rack 205 is mounted on the fixed block 209, and the fixed block 209 is fixed to the one-dimensional moving device 100.
As shown in fig. 2, the automatic biological tactile sensation experiment rotation module 300 adopts an automatic experimental biological rotation device in a standard environment; the automatic experimental organism switching device comprises a second rotating platform 302 and a second rotating disc 301, wherein n second round holes for placing the biological fixing devices 303 are uniformly formed in the second rotating disc 301, the biological fixing devices 303 are used for placing experimental organisms, and the experimental organisms can be bees and the like. In the embodiment of the present invention, the bio-fixation device 303 and the second turntable 301 are both made of ABS plastic, and in order to enable the bio-fixation device 303 to rotate, a rolling bearing 304 is assembled in the second circular hole of the second turntable 301, the bio-fixation device 303 is fixed with the adapter 530, and the adapter 530 is matched with the rolling bearing 304. A two-dimensional moving device 400 for driving the second rotating platform 302 to move in the X-axis direction and the Y-axis direction is arranged below the second rotating platform 302, the two-dimensional moving device 400 is connected with the second rotating platform 302 of the experimental biological automatic switching device 300, and the two-dimensional moving device 400 drives the second rotating platform 302 to move in the X-axis direction and the Y-axis direction; through the arrangement of the two-dimensional moving device 400, the position adjustment of the whole experimental organism automatic switching device in the X-axis and Y-axis directions is realized, so that the reagent tube can be accurately aligned with the experimental organism fixed in a certain biological fixing device 303; the two-dimensional moving device 400 is formed by overlapping two horizontal ball screw sliding table mechanisms (410, 420). Specifically, in the present embodiment, two horizontal ball screw sliding table mechanisms (410, 420) are stacked via the slider 411, and the two horizontal ball screw sliding table mechanisms (410, 420) are vertically disposed. The base of the horizontal ball screw sliding table mechanism (410, 420) is designed to be 500mm long, 140mm wide, 135mm guide rail spacing, 300mm guide rail long and 10mm high. The screw 412 is coaxially assembled with a coupling 413, a second motor 414 and a bearing 415, and the bearing 415 is fixed on a base 416. The rolling bearing 415 is 618-8 GB 276-94, the inner diameter is 8mm, and the outer diameter is 16 mm. The second motor 414 is 56 x 76mm in size. In a further embodiment, the screw 412 drives the sliding screw platform 303 to move, the table surface of the sliding screw platform 303 coincides with the table surface of the electric indexing disc rotating table (the second rotating platform 302), the diameter of the electric indexing disc rotating table (the second rotating platform 302) is 150mm, the diameter of the circular hole on the second rotating disc 301 is 10mm, and 10 second circular holes are circumferentially arrayed at a position 54mm away from the center of the second rotating disc 301.
Preferably, the two-dimensional moving device 400 is provided with a sliding screw platform 305 above the horizontal ball screw sliding table mechanism 410 at the upper end, and the sliding screw platform 305 is provided with an L-shaped structure. In the embodiment of the present invention, the second turntable 301 is connected and coaxially engaged with an electric indexing disc rotating table (second rotating table 302) through a sliding screw platform 305, a rotating motor of the electric indexing disc rotating table is connected with the electric indexing disc rotating table (second rotating table 302) through a coupling, and when the rotating motor is started, the electric indexing disc rotating table (second rotating table 302) rotates to drive the second turntable 301 to rotate.
The antenna position alignment module comprises an image acquisition processing device and an angle adjusting device; the image acquisition and processing device is arranged above an insect training standard environment and comprises an industrial camera and image acquisition software corresponding to the industrial camera, the industrial camera realizes photographing of the tentacle position of a living being (bee), the image acquisition software realizes processing and analysis of the acquired image, and the angle deviation between the central line of two tentacles of the living being (bee) and the reference line of the biological fixing device is calculated; in this embodiment, the industrial camera is a large constant MER-500-7UM camera, and the corresponding image acquisition software is matlab software. The angle adjusting device 500 comprises a fine adjusting device, the fine adjusting device comprises an adapter 530 fixed below the biological fixing device 303 and a power device, the power device comprises a linear rotating motor 510 and a rotary adjusting workpiece 520, and the adapter 530 is used for connecting the biological fixing device 303 and the power device. Preferably, in the present invention, a linear rotating motor 510 is adopted, the linear rotating motor 510 drives a rotation adjusting workpiece 520 to move linearly up and down, so as to realize the butt joint with the adaptor 530, and in addition, the linear rotating motor drives the rotation adjusting workpiece 520 to perform a rotational movement, so as to realize the rotation of the adaptor 530 and the biological fixing device 303 fixed above the adaptor 530 by a certain angle, so as to realize the function of correcting the head position deviation of the living being (bee).
As shown in fig. 7, a schematic diagram of a deviation image of a position of a living being (bee) is shown, specifically, in this embodiment, an industrial camera photographs an antenna position of the living being (bee), and processes and analyzes the acquired image through corresponding image acquisition software to calculate a deviation angle between two antenna center lines of the living being (bee) and an angle position of a reference line of a biological fixing device.
Fig. 4 shows a connection diagram of the bio-fixation device 303 and the adaptor device 530, fig. 5 shows the adaptor device 530, and the bio-fixation device 303 has a cavity for accommodating the experimental organism, and the cavity is a through hole. Specifically, as shown in fig. 6, the rotation adjusting component 520 is fixedly connected to the output shaft of the linear rotating motor 510 through a connecting member 540, and the rotation adjusting component 520 has a connecting protrusion 521. The upper end of the adapter 530 is fixedly connected to the lower portion of the accommodating cavity, and it is feasible that the adapter 530 and the biological fixing device 303 can be connected by an integral forming method, a snap connection method, a butt connection method or a bolt connection method, and the connecting groove 531 matched with the connecting protrusion 521 is arranged below the adapter 530. The upper part of the switching device 530 is provided with a semicircular groove for connecting with the biological fixing device 303 to play a fixing role, the lower end is also provided with a connecting groove 531 to form a semicircular structure, the semicircular connecting groove 531 is butted with a connecting lug 521 of the semicircular structure at the upper end of the power device, and the power device is convenient to drive the switching device 530 and the biological fixing device 303 to rotate and not slip. The upper end of the biological fixing device 303 is designed as a standard semicircle, and the camera photographs the semicircle position to determine the initial position of the organism (bee) before fine adjustment.
Specifically, the connecting member 540 is connected to an output shaft of the linear rotating motor 510 to drive the rotation adjusting workpiece 520 to rotate and rise, and when the upper end of the rotation adjusting workpiece 520 contacts with the lower connecting groove 531 of the adapter 530, the connecting member 540 and the rotation adjusting workpiece 520 rotate synchronously, so that the position of the experimental creature (bee) is corrected. When the experimental creatures (bees) are rotated, the linear rotating motor 510 rotates reversely to drive the rotation adjusting workpiece 520 to rotate and descend, and then the rotation adjusting workpiece returns to the safe position during rotation. Preferably, in the present invention, the lower end of the rotation adjusting member 520 is formed in a shaft shape, and the connecting member 540 may be a coupling to connect the rotation adjusting member 520 with the output shaft of the linear rotating motor 510.
In this embodiment, the reagent tube 203 is filled with liquid reagent, the reagent is electrically connected to a ground terminal of a signal acquisition device through a wire, the organism (bee) is electrically connected to an input terminal of a data acquisition card in the signal acquisition device, when the organism (bee) antenna contacts with the reagent, an electrical signal is generated, and the data acquisition card acquires the electrical signal and transmits the acquired signal to a computer.
The working process of the specific embodiment of the invention is as follows: a detection method for researching biological antenna perception mechanism is as follows:
step S1, providing a detection device for researching a biological antenna sensing mechanism; the rotation adjusting member 520 is mounted on the linear rotating motor 510, and the bio-fixation device 303 is fixed to the adaptor 530.
Step S2, fixing the organism (bee) on the organism fixing device 303, connecting the organism (bee) with one input end of the signal acquisition card in the signal acquisition device by a lead wire, covering the visual organs of the organism (bee), placing the reagent tube 203 in the first round hole of the first turntable 202, and fixing the armature 204 at the tail end.
Step S3, connecting the liquid reagent stored in the reagent tube 203 with the grounding end of the data acquisition device through a lead to provide a grounding signal; the biological (bee) is electrically connected with a certain input end of a data acquisition card in the signal acquisition device, when the biological (bee) antenna is contacted with the reagent, an electric signal is generated, the contact bioelectric signal is mainly acquired by the data acquisition card of the signal acquisition device, and the data acquisition card transmits the acquired bioelectric signal to a computer.
Step S4, the two second motors 414 of the two-dimensional moving device 400 are started, and the two horizontal ball screw sliding table mechanisms (410, 420) of the two-dimensional moving device 400 drive the second rotating platform 302 to move to the designated position by using the sliding table screw platform 305.
Step S4 is specifically as follows:
step S401, the second rotary platform 302 rotates to drive the second rotary disc 301 to rotate, and the biological fixing device 303 is rotated to one of the biological fixing devices 303 which is positioned on the same straight line with the reagent tube 204 at the lowest end of the first rotary disc 202;
s402, an industrial camera photographs the position of a living being (bee), and the angle deviation between the central line of two antennae of the living being (bee) and the reference line of the fixing device is calculated as an angle by using software;
step S403, after the linear rotating motor 510 is started to rotate by a certain angle, the output shaft of the linear rotating motor 510 moves upwards, so that the rotation adjusting workpiece 520 is in butt joint with the adapter 530, and after the power device drives the biological fixing device 303 to rotate reversely by the adapter 530, the output shaft of the linear rotating motor contracts downwards, so that the rotation adjusting workpiece 520 is separated from the adapter and returns to a safe position.
Step S5, the second motor 414 of the one-dimensional moving device is started, and the vertical ball screw drives the first rotating platform 201 to move to the designated position.
Step S5 is specifically as follows:
step S501, the first rotating platform 201 rotates to drive the first rotating disc 202 to rotate, and one of the reagent tubes 203 is rotated to the lowest point position of the first rotating disc 202;
step S502, electrifying the electromagnetic magnet 207, starting a first motor 208 of the inducer automatic stretching device to drive a gear 206 to rotate, driving the gear 206 to transmit to a rack 205, pushing the electromagnetic magnet 207 to move forwards, attracting the electromagnetic magnet 207 with an armature 204, thereby pushing the armature 204 and a reagent tube 203 out through the rotation of the first motor 208 of the inducer automatic stretching device, pushing the armature to a position with a small antenna distance D (D value) with a living being (bee), stopping the first motor 208, enabling the living being (bee) to randomly move in a space range by utilizing the space search characteristic of the antenna of the living being (bee), recording contact electric signals of the antenna of the collected living being (bee) and transmitting the signals to a computer when the living being (bee) is in first contact with the reagent tube 203, and simultaneously shooting and recording the motion behavior track of the antenna by an industrial camera;
step S503, after the signal acquisition is finished, the first motor 208 of the inducer automatic telescoping device rotates reversely, the reagent tube 203 retracts, the coil of the electromagnetic magnet 207 is powered off, the armature 204 is separated from the iron core of the electromagnetic magnet 207, the rack 205 retracts to a safe position, and the first motor 208 stops.
And step S6, repeating the steps, starting an electric dividing disc rotating table of the inducer automatic rotating device to drive the first rotating disc 202 to rotate, and sequentially contacting the m reagent tubes 203 with the biological (bee) antennae for an experiment to complete the research on the perception mechanism of the same biological (bee) antennae for different reagents.
And step S7, repeating the steps, starting an electric dividing disc rotating table of the experimental biological automatic conversion device to drive the second rotating disc 301 to rotate, and enabling n organisms (bees) to be in contact with the same reagent pipe 203 in sequence to carry out experiments, so that the research on the sensing mechanism of the same reagent by the antennae of different organisms (bees) is completed.
While the foregoing is directed to the preferred embodiment of the present invention, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (8)
1. A detection device for biological antenna perception mechanism research is characterized by comprising an induction switching module, a biological tactile experiment automatic alternation module and an antenna position alignment module;
the induction switching module comprises an automatic inducer switching device and an automatic inducer telescoping device; the automatic inducer switching device comprises a first rotating platform and a first rotating disc arranged on the first rotating platform, wherein m first round holes for placing reagent tubes are uniformly formed in the first rotating disc; the automatic inducer telescoping device comprises a gear, a rack and an electromagnetic magnet, the gear is meshed with the rack and is fixedly connected with the rack, the electromagnetic magnet consists of an iron core, a coil and an armature which is detachably connected to the iron core, the armature is sleeved on a reagent tube, the iron core generates electromagnetic attraction and attracts the armature under the condition that the coil is electrified, the iron core loses magnetic force and is disconnected from the armature to adsorb under the condition that the coil is powered off, the gear is driven to rotate by a first motor, the gear is meshed with the rack to drive the electromagnetic magnet, and the iron core of the electromagnetic magnet adsorbs the armature to drive the reagent tube to stretch and move under the condition that the coil is powered on;
the biological touch sensation experiment automatic alternation module adopts an experiment biological automatic alternation device in a standard environment; the experimental organism automatic conversion device comprises a second rotating platform and a second rotating disc, wherein a plurality of second round holes for placing the biological fixing devices are uniformly formed in the second rotating disc, a biological fixing device is sleeved in each second round hole, and the biological fixing devices are used for placing experimental organisms;
the antenna position alignment module comprises an image acquisition processing device and an angle adjusting device; the image acquisition and processing device is arranged above the insect training standard environment and comprises an industrial camera and image acquisition software corresponding to the industrial camera, the industrial camera realizes the photographing of the tentacle positions of bees, the image acquisition software realizes the processing and analysis of the acquired images and calculates the angle deviation between the central lines of the two tentacles of the bees and the reference line of the biological fixing device; the angle adjusting device comprises a fine adjusting device, the fine adjusting device comprises a switching device and a power device which are fixed below the biological fixing device, the power device comprises a linear rotating motor and a rotary adjusting workpiece, the linear rotating motor is used for realizing axial linear motion and rotary motion, the linear rotating motor is fixedly connected with the rotary adjusting workpiece, an output shaft of the linear rotating motor drives the rotary adjusting workpiece to realize butt joint and separation with the switching device along the linear direction, the output shaft of the linear rotating motor starts rotary motion after the rotary adjusting workpiece is in butt joint with the switching device, the output shaft of the linear rotating motor adjusts the calculated angle deviation between the central line of the two contact angles of the bees and the reference line of the biological fixing device in the rotating process, and the linear rotating motor drives the switching device to adjust the bees to the correct position convenient for contacting with the reagent, the switching device is used for connecting the biological fixing device and the power device.
2. The detecting device for the research on the biological antenna sensing mechanism as claimed in claim 1, wherein the rotation adjusting workpiece is fixedly connected to the output shaft of the linear rotating motor through a connecting piece, and the rotation adjusting workpiece is provided with a connecting lug.
3. The detecting device for the research on the mechanism of biological antenna sensing according to claim 1, wherein the biological fixing device has a cavity for accommodating the experimental organism, and the cavity is a through hole.
4. The detecting device for the research on the mechanism of biological antenna sensing according to claim 2, wherein the upper end of the adapter is fixedly connected to the lower portion of the accommodating cavity, and the lower portion of the adapter has a connecting groove matched with the connecting protrusion.
5. The detecting device for the research on the mechanism of biological antenna perception according to claim 1, further comprising a moving device, wherein the moving device comprises a one-dimensional moving device for moving the first rotating platform and a two-dimensional moving device for moving the second rotating platform, the one-dimensional moving device is connected with the first rotating platform of the inducer automatic rotating device, the one-dimensional moving device drives the first rotating platform to move along the Z-axis direction, the two-dimensional moving device is connected with the second rotating platform of the experimental biological automatic rotating device, and the two-dimensional moving device drives the second rotating platform to move along the X-axis direction and the Y-axis direction.
6. The detecting device for the research of the biological antenna sensing mechanism according to claim 5, wherein the one-dimensional moving device is a vertical ball screw sliding table mechanism, and the two-dimensional moving device is formed by overlapping two horizontal ball screw sliding table mechanisms.
7. The detection device for the research on the biological antenna sensing mechanism according to claim 6, wherein the vertical ball screw sliding table mechanism and the two horizontal ball screw sliding table mechanisms are driven by a second motor.
8. The detecting device for researching biological antenna sensing mechanism as claimed in claim 1, wherein said reagent tube is filled with liquid reagent, said liquid reagent is electrically connected to the ground terminal of the signal collecting device through a conducting wire, the bee is electrically connected to an input terminal of a data collecting card in the signal collecting device, when the bee antenna contacts with the reagent, a biological electrical signal is generated, said data collecting card collects the biological electrical signal through an input terminal of said signal collecting device, and transmits the collected biological electrical signal to the computer.
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