CN112275166A

CN112275166A - Nutrient solution mixing device for cell culture

Info

Publication number: CN112275166A
Application number: CN202011102528.1A
Authority: CN
Inventors: 陈永杰
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-10-15
Filing date: 2020-10-15
Publication date: 2021-01-29

Abstract

The invention discloses a nutrient solution mixing device for cell culture, which comprises a shell and an inner cylinder capable of floating up and down in the shell, wherein the inner cylinder comprises a first liquid cavity facing the bottom of the shell and a second liquid cavity facing the top of the shell, the inner cylinder is provided with a liquid inlet channel which flows in a one-way manner from the second liquid cavity to the first liquid cavity, the bottom of the shell is provided with a circulating pipe, the top of the circulating pipe is provided with a first piston which is in sealing sliding fit with the first liquid cavity, the first piston is provided with a liquid outlet channel which flows in a one-way manner from the first liquid cavity to the circulating pipe, the second liquid cavity is internally provided with a second piston which is in sealing sliding fit, the second piston drives the inner cylinder to move down when moving down to the bottom of the second liquid cavity, and the second piston drives the inner cylinder to move up when moving up to the top of the second liquid. The invention aims to provide a nutrient solution mixing device for cell culture, which can mix raw materials when liquid is added every time and prevent waste.

Description

Nutrient solution mixing device for cell culture

Technical Field

The invention belongs to the field of cell culture, and particularly relates to a nutrient solution mixing device for cell culture.

Background

In the field of cell culture, devices such as a cell culture machine are generally used, a liquid inlet pipeline or an injector needs to be arranged in the cell culture machine to fill a specific culture vessel with mixed nutrient solution, the mixed nutrient solution generally needs to be filled into a liquid filling cylinder of the cell culture machine after two raw materials are mixed in an external environment, the mixed nutrient solution is easy to deteriorate, and due to the fact that more nutrient solutions can be mixed at one time, the mixed nutrient solution can only be discarded after deterioration occurs, and a large amount of waste situations are caused.

Disclosure of Invention

In order to overcome the defect that the nutrient solution is easy to deteriorate and cause waste after being mixed in the prior art, the invention provides the nutrient solution mixing device for cell culture, which can mix raw materials when adding liquid every time and prevent the waste.

In order to achieve the purpose, the invention adopts the following technical scheme:

the utility model provides a nutrient solution mixing arrangement for cell culture, includes the shell and can the inner tube that floats from top to bottom in the shell, the inner tube is including the first sap cavity towards the shell bottom and towards the second sap cavity at shell top, the inner tube is equipped with the inlet channel of the one-way circulation of second sap cavity to first sap cavity, the bottom of shell is equipped with the runner pipe, the top of runner pipe is equipped with the first piston with the sealed sliding fit of first sap cavity, first piston is equipped with the play liquid channel from first sap cavity to the one-way circulation of runner pipe, be equipped with sealed sliding fit's second piston in the second sap cavity, the second piston drives the inner tube when moving down to second sap cavity bottom and moves down, the second piston moves up when moving to second sap cavity top and moves up the inner tube. When the second piston moves downwards, the second liquid cavity is compressed, raw materials in the second liquid cavity are injected into the first liquid cavity, so that the two raw materials of the first liquid cavity and the second liquid cavity are mixed, when the second piston moves downwards to the bottom of the second liquid cavity, the inner cylinder is driven to move downwards, and because the first piston and the shell are kept relatively immobile, when the inner cylinder moves downwards, the first piston moves upwards relative to the inner cylinder, and mixed liquid in the first liquid cavity is pressed into the circulation pipe to be discharged. Like this, just mix two kinds of feed liquor before the ration feed liquor of every turn, then carry out the operation of feed liquor filling, avoid the nutrient solution after the mixture to take place the condition about rotten, can effectively prevent the waste of nutrient solution.

Preferably, the first liquid cavity is provided with a rotating sheet at the outlet of the liquid inlet channel, the middle of the rotating sheet is provided with a liquid outlet communicated with the liquid inlet channel, the rotating sheet rotates along the axial direction of the first liquid cavity, and the rotating sheet extends from the liquid outlet to the inner wall of the first liquid cavity to form a spoon-shaped guide fan blade. Therefore, when liquid in the second liquid cavity flows to the first liquid cavity from the liquid inlet channel, the liquid flow passes through the spoon-shaped guide fan blades to drive the rotating sheet to rotate, and the rotating sheet rotates to help to uniformly stir and mix the liquid of the two raw materials.

Preferably, the diameter of the liquid outlet is contracted from the liquid inlet channel to the first liquid cavity, and one end of the flow guide fan blade, which is close to the liquid outlet, is provided with a flow guide bulge arranged along the edge of the flow guide fan blade. The liquid outlet of shrink helps improving the liquid flow velocity of flow, through set up the water conservancy diversion arch at water conservancy diversion flabellum edge, plays better water conservancy diversion effect to improve the rotation efficiency of rotor plate, guarantee to mix the effect.

Preferably, transmission gears are respectively arranged on two sides of the inner cylinder in the shell, an inner rack in matched transmission with the transmission gears is arranged on the side wall of the inner cylinder, a connecting rod is arranged on the second piston, a connecting block is arranged at the top of the connecting rod, and an outer rack in matched transmission with the transmission gears is arranged on the connecting block. The outer rack and the inner rack are respectively meshed with the transmission gear, when the second piston moves downwards, the outer rack moves the transmission gear to rotate to enable the inner rack to move upwards through the connecting rod and the connecting block, and therefore the inner cylinder moves upwards, the volume of the first liquid cavity is increased, and liquid in the first liquid cavity is mixed favorably.

Preferably, a strip-shaped groove corresponding to the transmission gear is formed in the shell, a positioning sliding block is arranged in the strip-shaped groove, the positioning sliding block is released from being fixed relative to the strip-shaped groove when contacting with the connecting block, the transmission gear is rotatably arranged on the positioning sliding block, and a sliding block spring is arranged between the bottom of the strip-shaped groove and the positioning sliding block. When the positioning slide block is in an unlocking state, the positioning slide block can slide in the strip-shaped groove, and meanwhile, the second piston also drives the inner cylinder to move downwards to extrude and discharge the mixed liquid in the first liquid cavity downwards.

Preferably, the positioning slide block comprises a slide block body and an unlocking button which is located in the slide block body and slides in the vertical direction, a positioning pin linked with the unlocking button is arranged in the slide block body, and a locking groove matched with the positioning pin is formed in the upper end of the strip-shaped groove. When the unlocking button is not pressed, the positioning pin and the locking groove are positioned to enable the positioning slide block and the strip-shaped groove to be relatively fixed, and when the unlocking button is pressed to move downwards, the positioning pin and the locking groove are released through linkage.

As preferred, be equipped with the dwang in the location slider, the lower extreme of unblock button is equipped with and acts on the contact lever of dwang downwardly pivoted, be equipped with the guide post on the locating pin, the lower extreme of dwang be equipped with guide post sliding fit's guide way, the locking groove is kept away from to drive the locating pin when dwang is turned down. Through the linkage cooperation of contact lever, dwang and locating pin, thereby make the locating pin can take place horizontal slip and fix or remove the position locking with the cooperation of locking groove.

Preferably, the bottom of the positioning sliding block is provided with a horizontal sliding groove, the positioning pin horizontally slides in the sliding groove, one end, facing the locking groove, of the positioning pin is arc-shaped, and a positioning pin spring for pushing the positioning pin to move towards the locking groove is arranged in the sliding groove. When the positioning slide block moves upwards and returns, the positioning pin can reset under the action of the positioning pin spring and enter the locking groove to realize automatic locking and positioning.

The invention has the beneficial effects that: (1) the nutrient solution is mixed in a small amount at a time, so that the deterioration and waste of the nutrient solution are avoided, and the cost of raw materials is saved; (2) the mixing of the two raw materials is completed in the extraction and injection processes, the extraction and injection processes are reliable and stable, and the liquid adding efficiency is improved.

Drawings

FIG. 1 is a schematic diagram of an embodiment of the present invention;

FIG. 2 is a side view of the present invention;

FIG. 3 is an enlarged view of a portion of FIG. 1 at A;

FIG. 4 is a schematic structural view of the positioning block of the present invention in a locked state;

FIG. 5 is a schematic structural view of the positioning block of the present invention in an unlocked state;

FIG. 6 is a schematic view of the second piston of the present invention sliding to the bottom of the second chamber;

FIG. 7 is a schematic view of the first piston of the present invention sliding to the bottom of the first chamber.

In the figure: the liquid inlet valve comprises a flow pipe 1, a first piston 2, a liquid outlet channel 2a, a slider spring 3, a strip-shaped groove 4, a transmission gear 5, a positioning slider 6, a rotating rod 61, a guide groove 61a, an unlocking button 62, a contact rod 63, a slider body 64, a sliding groove 65, an external toothed bar 7, a second piston 8, an action part 9, a connecting block 10, a shell 11, a locking groove 11a, a connecting rod 12, an end cover 13, an inner cylinder 14, a first liquid cavity 141, a second liquid cavity 142, a liquid inlet channel 14a, an inner toothed bar 14b, a second one-way valve 15, a rotating piece 16, a flow guide bulge 161, a flow guide fan blade 162, a liquid outlet 163, a first one-way valve 17, a fourth one-way valve 18, a second liquid inlet pipeline 19, a first liquid inlet pipeline 20, a third one-way valve 21, a positioning pin 22.

Detailed Description

The invention is further described with reference to the following figures and detailed description.

A nutrient solution mixing device for cell culture comprises a shell 11 and an inner barrel 14, wherein the inner barrel 14 is positioned in the shell 11, the inner barrel 14 comprises a first liquid cavity 141 and a second liquid cavity 142, the first liquid cavity 141 is positioned at the lower end of the inner barrel 14, the first liquid cavity 141 faces the bottom of the shell 11, the second liquid cavity 142 is positioned at the upper end of the inner barrel 14, and the second liquid cavity 142 faces the top of the shell 11. The bottom of the shell 11 is provided with a circulating pipe 1, the circulating pipe 1 is arranged in the vertical direction, the top of the circulating pipe 1 is provided with a first piston 2, and the first piston 2 is in sealing sliding fit with the first liquid cavity 141, so that the up-and-down floating of the inner cylinder 14 in the shell 11 is realized. The first piston 2 is provided with a liquid outlet channel 2a, the liquid outlet channel 2a is provided with a first one-way valve 17, the first one-way valve 17 enables the first liquid cavity 141 to keep one-way circulation to the circulation pipe 1, and when the inner cylinder 14 moves downwards relative to the first piston 2, the first piston 2 compresses the first liquid cavity 141, so that the liquid in the first liquid cavity 141 is discharged from the circulation pipe 1.

A second piston 8 in sealed sliding fit is arranged in the second liquid cavity 142, an inlet channel 14a is arranged between the second liquid cavity 142 and the first liquid cavity 141 of the inner barrel 14, a second one-way valve 15 is arranged in the inlet channel 14a, so that the second liquid cavity 142 is communicated with the first liquid cavity 141 in one way, and when the second piston 8 moves downwards relative to the second liquid cavity 142, the second piston 8 compresses the space of the second liquid cavity 142, so that the liquid in the second liquid cavity 142 flows into the first liquid cavity 141. The bottom of the second liquid cavity 142 is provided with a plane which is contacted with the second piston 8, and the inner cylinder 14 is driven to move downwards when the second piston 8 moves downwards to the bottom of the second liquid cavity 142. An end cover 13 is arranged at the opening of the top of the second liquid chamber 142, and when the second piston 8 moves up to the top of the second liquid chamber 142, the end cover 13 is abutted to drive the inner cylinder 14 to move up.

In addition, as shown in fig. 2, the inner cylinder 14 is provided with a first liquid inlet pipe 20 at the bottom of the first liquid cavity 141, and a third one-way valve 21 is provided at a communication position between the first liquid inlet pipe 20 and the first liquid cavity 141, so as to ensure one-way communication between the first liquid inlet pipe 20 and the first liquid cavity 141. The inner cylinder 14 is provided with a second liquid inlet pipeline 19 at the bottom of the second liquid cavity 142, and a fourth check valve 18 is provided at the communication position of the second liquid inlet pipeline 19 and the second liquid cavity 142, so as to ensure the one-way circulation between the second liquid inlet pipeline 19 and the second liquid cavity 142.

Referring to fig. 3, the first liquid chamber 141 is provided with a rotating plate 16 at an outlet of the liquid inlet channel 14a, a liquid outlet 163 communicated with the liquid inlet channel 14a is provided in a middle portion of the rotating plate 16, the rotating plate 16 rotates along an axial direction of the first liquid chamber 141, and the rotating plate 16 extends from the liquid outlet 163 to an inner wall of the first liquid chamber 141 to form a scoop-shaped guide vane 162. The diameter of the liquid outlet 163 is reduced from the liquid inlet channel 14a to the first liquid cavity 141, and a flow guiding protrusion 161 arranged along the edge of the flow guiding fan 162 is disposed at one end of the flow guiding fan 162 close to the liquid outlet 163. The diversion fan blades 162 are spoon-shaped, and when the liquid flow of the liquid outlet 163 impacts on the diversion fan blades 162, the rotation piece 16 is driven to rotate integrally. The rotating plate 16 stirs the liquid in the first liquid chamber 141 during the rotation process, so that the two materials are mixed more uniformly.

As shown in fig. 4, transmission gears 5 are respectively arranged on two sides of an inner cylinder 14 inside a housing 11, an inner rack 14b in transmission matched with the transmission gears 5 is arranged on the side wall of the inner cylinder 14, a vertical connecting rod 12 is arranged on a second piston 8, a connecting block 10 is arranged on the top of the connecting rod 12, and an outer rack 7 in transmission matched with the transmission gears 5 is arranged on the connecting block 10. In this embodiment, the transmission gear 5 is coaxially fixed by a pinion and a gearwheel, wherein the gearwheel is engaged with the outer rack 7, the pinion is engaged with the inner rack 14b, and the reference circle diameter of the pinion is determined according to the ratio of the cross-sectional areas of the first fluid chamber 141 and the second fluid chamber 142, so that the fluid in the second fluid chamber 142 does not flow out of the flow pipe 1 when the fluid in the second fluid chamber 142 flows into the first fluid chamber 141 and is mixed. The inner rack 14b and the outer rack 7 are located on both sides of the transmission gear 5, and are arranged in parallel, and when the transmission gear 5 rotates, the inner rack 14b and the outer rack 7 move in opposite directions.

Combine fig. 4, fig. 5 to show, the inside bar groove 4 that corresponds with drive gear 5 that is equipped with of shell 11, bar groove 4 arranges along vertical direction, is equipped with location slider 6 in the bar groove 4, and drive gear 5 rotates and sets up on location slider 6, is equipped with slider spring 3 between 4 bottoms in bar groove and the location slider 6. The positioning slide block 6 comprises a slide block body 64 and an unlocking button 62 which is positioned in the slide block body 64 and slides up and down, the connecting block 10 extends downwards to an action part 9 which can be contacted with the unlocking button 62, a positioning pin 22 linked with the unlocking button 62 is arranged in the slide block body 64, and a locking groove 11a matched with the positioning pin 22 is arranged at the upper end of the strip-shaped groove 4. Be equipped with dwang 61 in the location slider 6, the lower extreme of unblock button 62 is equipped with and acts on dwang 61 pivoted contact lever 63 downwards, is equipped with guide post 22a on the locating pin 22, the lower extreme of dwang 61 be equipped with guide post 22a sliding fit's guide way 61a, drive locating pin 22 when dwang 61 is pivoted downwards and keep away from locking groove 11a, remove when realizing location slider 6 and connecting block 10 contact with bar groove 4 relatively fixed. The bottom of the positioning slide block 6 is provided with a horizontal sliding groove 65, the positioning pin 22 horizontally slides in the sliding groove 65, one end of the positioning pin 22 facing the locking groove 11a is arc-shaped, and a positioning pin spring 23 for pushing the positioning pin 22 to move towards the locking groove 11a is arranged in the sliding groove 65.

In the actual operation process, the second piston 8 moves downwards under the action of external force to compress the second liquid chamber 142, at the moment, because the positioning slide block 6 is in the position locking state, the positioning slide block 6 and the strip-shaped groove 4 are kept relatively fixed, and the raw materials in the second liquid chamber 142 are injected into the first liquid chamber 141 by the second piston 8, so that the two raw materials in the first liquid chamber 141 and the second liquid chamber 142 are mixed. Meanwhile, due to the transmission of the outer rack 7, the transmission gear 5 and the inner rack 14b, the inner cylinder 14 moves upwards under the driving of the inner rack 14b, so that the liquid in the second liquid chamber 142 enters and is mixed with the liquid in the first liquid chamber 141. As shown in fig. 7, when the second piston 8 moves down to the bottom of the second liquid chamber 142, the connecting block 10 contacts with the upper end of the positioning slider 6, and presses the unlocking button 62 to move down, so that the positioning slider 6 and the strip-shaped groove 4 are switched to a relative sliding state, the second piston 8 pushes the inner cylinder 14 to move down, the first piston 2 moves up relative to the inner cylinder 14 when the inner cylinder 14 moves down, and the mixed liquid in the first liquid chamber 141 is pressed into the circulation pipe 1 to be discharged. When the last liquid adding is needed after the single liquid adding is completed, the second piston 8 and the inner barrel 14 move upwards until the positioning slide block 6 moves to the upper end of the strip-shaped groove 4 for locking and positioning. In the process that the second piston 8 and the inner barrel 14 move upwards, the first liquid inlet pipeline 20 and the second liquid inlet pipeline 19 are opened in a single direction, and raw materials are filled into the first liquid cavity 141 and the second liquid cavity 142 respectively, so that subsequent liquid adding is facilitated.

Claims

1. A nutrient solution mixing device for cell culture is characterized by comprising a shell (11) and an inner barrel (14) capable of floating up and down in the shell (11), wherein the inner barrel (14) comprises a first liquid cavity (141) facing the bottom of the shell (11) and a second liquid cavity (142) facing the top of the shell (11), the inner barrel (14) is provided with a liquid inlet channel (14 a) which flows in a one-way manner from the second liquid cavity (142) to the first liquid cavity (141), the bottom of the shell (11) is provided with a circulating pipe (1), the top of the circulating pipe (1) is provided with a first piston (2) which is in sealed sliding fit with the first liquid cavity (141), the first piston (2) is provided with a liquid outlet channel (2 a) which flows in a one-way from the first liquid cavity (141) to the circulating pipe (1), and a second piston (8) which is in sealed sliding fit is arranged in the second liquid cavity (142), when the second piston (8) moves downwards to the bottom of the second liquid cavity (142), the inner cylinder (14) is driven to move downwards, and when the second piston (8) moves upwards to the top of the second liquid cavity (142), the inner cylinder (14) is driven to move upwards.

2. The nutrient solution mixing device for cell culture as claimed in claim 1, wherein the first liquid cavity (141) is provided with a rotating plate (16) at the outlet of the liquid inlet channel (14 a), the middle part of the rotating plate (16) is provided with a liquid outlet (163) communicated with the liquid inlet channel (14 a), the rotating plate (16) rotates along the axial direction of the first liquid cavity (141), and the rotating plate (16) extends from the liquid outlet (163) to the inner wall of the first liquid cavity (141) to form a spoon-shaped guide fan blade (162).

3. The mixing apparatus of claim 2, wherein the diameter of the liquid outlet (163) is reduced from the liquid inlet channel (14 a) to the first liquid chamber (141), and the end of the guide vane (162) near the liquid outlet (163) is provided with a guide protrusion (161) disposed along the edge of the guide vane (162).

4. The nutrient solution mixing device for cell culture as claimed in claim 1, wherein transmission gears (5) are respectively arranged at two sides of the inner cylinder (14) inside the housing (11), an inner rack (14 b) which is in matched transmission with the transmission gears (5) is arranged on the side wall of the inner cylinder (14), the second piston (8) is provided with a connecting rod (12), a connecting block (10) is arranged at the top of the connecting rod (12), and an outer rack (7) which is in matched transmission with the transmission gears (5) is arranged on the connecting block (10).

5. The nutrient solution mixing device for cell culture as claimed in claim 4, wherein a strip-shaped groove (4) corresponding to the transmission gear (5) is arranged in the shell (11), a positioning slide block (6) is arranged in the strip-shaped groove (4), the positioning slide block (6) is released from being fixed relative to the strip-shaped groove (4) when being contacted with the connecting block (10), the transmission gear (5) is rotatably arranged on the positioning slide block (6), and a slide block spring (3) is arranged between the bottom of the strip-shaped groove (4) and the positioning slide block (6).

6. The nutrient solution mixing device for cell culture as claimed in claim 5, wherein the positioning slide block (6) comprises a slide block body (64) and an unlocking button (62) which is positioned in the slide block body (64) and slides up and down, a positioning pin (22) which is linked with the unlocking button (62) is arranged in the slide block body (64), and a locking groove (11 a) which is matched with the positioning pin (22) is arranged at the upper end of the strip-shaped groove (4).

7. The nutrient solution mixing device for cell culture as claimed in claim 6, wherein a rotating rod (61) is arranged in the positioning slide block (6), a contact rod (63) acting on the rotating rod (61) to rotate downwards is arranged at the lower end of the unlocking button (62), a guide post (22 a) is arranged on the positioning pin (22), a guide groove (61 a) in sliding fit with the guide post (22 a) is arranged at the lower end of the rotating rod (61), and the positioning pin (22) is driven to be away from the locking groove (11 a) when the rotating rod (61) rotates downwards.

8. The nutrient solution mixing device for cell culture as claimed in claim 7, wherein the bottom of the positioning slide block (6) is provided with a horizontal sliding groove (65), the positioning pin (22) horizontally slides in the sliding groove (65), one end of the positioning pin (22) facing the locking groove (11 a) is arc-shaped, and a positioning pin spring (23) for pushing the positioning pin (22) to move towards the locking groove (11 a) is arranged in the sliding groove (65).