CN112266872A - A agitator for plant cell culture device - Google Patents
A agitator for plant cell culture device Download PDFInfo
- Publication number
- CN112266872A CN112266872A CN202011072235.3A CN202011072235A CN112266872A CN 112266872 A CN112266872 A CN 112266872A CN 202011072235 A CN202011072235 A CN 202011072235A CN 112266872 A CN112266872 A CN 112266872A
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- Prior art keywords
- liquid
- groove
- rotating
- cell culture
- plant cell
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/18—Flow directing inserts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/18—Flow directing inserts
- C12M27/22—Perforated plates, discs or walls
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/42—Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
Abstract
The invention discloses a stirrer for a plant cell culture device, which structurally comprises a motor, a stirring mechanism, a fixing plate, a controller and a lifting plate, wherein the stirring mechanism is provided with a fixing groove, a stirring device, a placing tank, a fixer and a blocking cover, liquid generates inward force under the action of rotating force to reduce the effect of the rotating force, the rule of the liquid rotating along the inner wall is destroyed through the dislocation of a circulating groove to ensure that the liquid circulates back and forth, the pressure of the liquid caused by the rotation is reduced, cells in the liquid are prevented from being broken, the liquid moves upwards along an inner side drainage groove of a sliding plate under the action of the rotating force to change the horizontal position of the liquid, the liquid expands outwards according to the mechanical principle, moves upwards along an inclined sealing groove under the action of the rotating force to compress the sealing groove, so that a stress block is stressed to ensure that the liquid on the inner side, the fluctuation generates upward pressure under the rotating force, thereby driving the liquid to be stirred upwards and improving the mixing degree of the liquid.
Description
Technical Field
The invention relates to the field of animal and plant cell culture devices, in particular to a stirrer for a plant cell culture device.
Background
Animal and plant cell culture adopts cell culture solution to stir, provides nutrient for plant cells to obtain nutrient in the culture solution, leads the cells to fully and uniformly contact with the culture solution through stirring, thereby achieving better culture effect, adopting an impeller baffle stirrer with a larger cut to reduce the shearing force of stirring, but the stirrer with the baffle plate structure can generate larger centrifugal force when the stirring speed is too high, so that the liquid rotates along the inner wall of the container, the pressure of plant cell chloroplasts is easy to generate, thereby causing the rupture of cell membranes and the loss of the activity of plant cells, and because the inner wall of the container is smooth, the liquid is easy to be attached and rotated in the rotating process, the fluctuation range of the horizontal position of the liquid at the same horizontal position is limited, and the liquid is not easy to be guided by the rotating force to be stirred upwards, so that the liquid mixing degree is low.
Disclosure of Invention
The invention is realized by the following technical scheme: a stirrer for plant cell culture device comprises a motor, a stirring mechanism, a fixing plate, a controller and a lifting plate, the motor is embedded and fixed at the upper end of the stirring mechanism, the upper end of the stirring mechanism is arranged on the side surface of the lifting plate, the controller is arranged on the side surface of the lifting plate, the lower end of the stirring mechanism is welded on the upper surface of the controller, the lifting plate is arranged at the upper end of the fixed plate, the stirring mechanism is provided with a fixed groove, a stirring device, a placing tank, a fixer and a blocking cover, the stirring device is arranged at the lower end of the fixer, the stirring device is positioned in the placing tank, the blocking cover is in threaded fit with the placing tank, the lower end of the placing tank is embedded and fixed in the fixing groove, the fixer is arranged on the side surface of the lifting plate, the motor is installed inside the fixer, and the stirring device and the fixing groove are integrally of a cylindrical structure.
As a further improvement of the invention, the stirring device is provided with a rotating shaft, connecting rods, rotating rods and rotating mechanisms, wherein the connecting rods are embedded and fixed outside the rotating shaft, the rotating rods are arranged outside the rotating shaft and positioned inside the rotating mechanisms, the rotating shafts are embedded and fixed at the lower ends of the fixing devices, the connecting rods and the rotating rods are respectively provided with four connecting rods and are distributed in a circular staggered manner, the rotating mechanisms are provided with two rotating mechanisms, the rotating mechanisms are respectively arranged outside the rotating rods and the connecting rods, and the diameters of the two rotating mechanisms are different.
The invention is further improved in that the rotating mechanism is provided with a circulation hole, a rotating ring, a fixing groove, a circulation groove and a connecting frame, the rotating ring is embedded and fixed in the middle of the connecting frame, the circulation hole and the rotating ring are of an integrated structure, the circulation groove is positioned on the inner side of the rotating ring, the fixing groove is embedded and fixed on the outer side of the rotating ring, the position of the circulation groove between the two rotating mechanisms is staggered, and the circulation hole is a round hole with a narrow middle and wide two sides.
As a further improvement of the invention, the rotating ring is provided with a curved block, a semicircular block and a circular ring, the curved block and the semicircular block are in an integrated structure, the semicircular block is attached to two sides of the circular ring, the circular ring is positioned in the middle of the connecting frame, the curved block and the semicircular block form a continuous bending structure, and the number of the semicircular blocks is two.
As a further improvement of the invention, the drainage device is provided with a tilting mechanism, a sliding plate and drainage grooves, wherein the drainage grooves are attached to the inner side of the sliding plate, the tilting mechanism is positioned on the inner side of the sliding plate, the sliding plate is embedded and fixed in the fixed groove, the inclination angles of the drainage grooves are the same, and the drainage grooves are of a continuous bending structure.
The invention is further improved in that the tilting mechanism is provided with a sealing groove, a stress block, a rubber plate and a fixing frame, wherein the sealing groove is positioned on the right side of the rubber plate, the stress block is attached to the left side of the fixing frame, the rubber plate is embedded and fixed on the left side of the fixing frame, the fixing frame is arranged on the inner side of the sliding plate, the sealing groove is of a tilting structure with a wider upper end and a narrower lower end, and the stress block is attached to the right side of the rubber plate.
Advantageous effects
Compared with the prior art, the invention has the beneficial effects that:
1. liquid fluctuates in the bent shape that curved shape piece in the rotating ring and semicircle piece formed, makes liquid produce the power to the inboard under the revolving force, reduces the effect of revolving force, and the dislocation through the circulation groove destroys the rotatory law along the inner wall of liquid, carries out the circulation each other between the liquid for liquid circulates back and forth, falls into two rotatory inner walls and has reduced rotatory pressure to liquid, avoids cell rupture in the liquid.
2. Along the inboard drainage groove upward movement of sliding plate under the effect of rotatory power, thereby change the horizontal position of liquid, liquid moves to the outside under the revolving force, when getting into tilt mechanism, according to the mechanics principle, liquid expands to the outside, along the seal groove upward movement of slope under the revolving force, compress the seal groove, thereby the atress piece receives pressure, make the inboard liquid of rubber slab produce undulantly, undulantly produce ascending pressure under the revolving force, thereby drive the upwards stirring of liquid, improve the mixed degree of liquid.
Drawings
FIG. 1 is a schematic view showing the construction of an agitator for a plant cell culture apparatus according to the present invention.
Fig. 2 is a schematic side view of a stirring mechanism according to the present invention.
Fig. 3 is a schematic top view of a stirring device according to the present invention.
Fig. 4 is a schematic side view of a rotating mechanism according to the present invention.
FIG. 5 is a schematic top view of a rotating ring according to the present invention.
Fig. 6 is a schematic perspective sectional view of a tank according to the present invention.
Fig. 7 is a schematic side view of a tilting mechanism according to the present invention.
In the figure: the device comprises a motor-1, a stirring mechanism-2, a fixed plate-3, a controller-4, a lifting plate-5, a fixed groove-21, a stirring device-22, a placing tank-23, a fixer-24, a blocking cover-25, a rotating shaft-221, a connecting rod-222, a rotating rod-223, a rotating mechanism-224, a circulating hole-a 1, a rotating ring-a 2, a fixed groove-a 3, a circulating groove-a 4, a connecting frame-a 5, a curved block-a 21, a semicircular block-a 22, a circular ring-a 23, an inclining mechanism-w 1, a sliding plate-w 2, a drainage groove-w 3, a sealing groove-w 11, a stressed block-w 12, a rubber plate-w 13 and a fixed frame-w 14.
Detailed Description
The technology of the present invention is further described with reference to the accompanying drawings:
example 1:
as shown in fig. 1-5:
the invention relates to a stirrer for a plant cell culture device, which structurally comprises a motor 1, a stirring mechanism 2, a fixing plate 3, a controller 4 and a lifting plate 5, wherein the motor 1 is embedded and fixed at the upper end of the stirring mechanism 2, the upper end of the stirring mechanism 2 is installed at the side surface of the lifting plate 5, the controller 4 is installed at the side surface of the lifting plate 5, the lower end of the stirring mechanism 2 is welded on the upper surface of the controller 4, the lifting plate 5 is installed at the upper end of the fixing plate 3, the stirring mechanism 2 is provided with a fixing groove 21, a stirring device 22, a placing tank 23, a fixer 24 and a blocking cover 25, the stirring device 22 is installed at the lower end of the fixer 24, the stirring device 22 is positioned in the placing tank 23, the blocking cover 25 is in threaded fit with the placing tank 23, the lower end of the placing tank 23 is embedded and fixed in the fixing groove 21, the fixer 24, the motor 1 is installed inside the fixer 24, and the stirring device 22 and the fixing groove 21 are integrally of a cylindrical structure, so that the plant culture solution in the holding tank 23 is not easy to splash out during stirring and is blocked by the blocking cover 25.
Wherein, agitating unit 22 is equipped with axis of rotation 221, connecting rod 222, dwang 223, rotary mechanism 224, connecting rod 222 inlays admittedly in the axis of rotation 221 outside, dwang 223 is installed in the axis of rotation 221 outside, dwang 223 is located rotary mechanism 224 inboard, axis of rotation 221 inlays admittedly at the 24 lower extreme of fixer, connecting rod 222 respectively installs four with dwang 223, and carries out circular dislocation distribution each other, rotary mechanism 224 is equipped with two, installs respectively in the outside of dwang 223 with connecting rod 222, and two rotary mechanism 224 diameter variation in size, avoids stirring in-process liquid to rotate along an inner wall for liquid makes a round trip to circulate, falls into two rotatory inner walls and has reduced the rotatory pressure to liquid.
The rotating mechanism 224 is provided with a flow hole a1, a rotating ring a2, a fixing groove a3, a flow groove a4 and a connecting frame a5, the rotating ring a2 is embedded and fixed in the middle of the connecting frame a5, the flow hole a1 and the rotating ring a2 are of an integrated structure, the flow groove a4 is positioned on the inner side of the rotating ring a2, the fixing groove a3 is positioned on the inner side of the rotating ring a2, the fixing groove a3 is embedded and fixed on the outer side of the rotating rod 223, the position of the flow groove a4 between the two rotating mechanisms 224 is staggered, the flow hole a1 is a round hole with a narrow middle and wide two sides, the liquid can rotate and flow more conveniently, and the rule of the liquid rotating along the inner wall is broken through the dislocation of the flow groove a4, so that the liquid can flow mutually.
The rotating ring a2 is provided with a curved block a21, a semicircular block a22 and a circular ring a23, the curved block a21 and the semicircular block a22 are of an integrated structure, the semicircular block a22 is attached to two sides of the circular ring a23, the circular ring a23 is located in the middle of the connecting frame a5, the curved block a21 and the semicircular block a22 form a continuous bending structure, and the number of the semicircular blocks a22 is two, so that liquid on the inner side and the outer side of the rotating ring a2 fluctuates, the rotating force is damaged, the effect of pressure generation is avoided while stirring is achieved, and cell breakage in liquid is avoided.
The embodiment has the following specific use modes and functions:
in the invention, the controller 4 rotates the motor 1 to drive the stirring device 22 to stir the liquid in the tank 23, the connecting rod 222 and the rotating rod 223 are driven by the rotating rod 211 to rotate in the stirring process, the circulating hole a1 in the rotating mechanism 224 is used for circulating the liquid, so that the liquid fluctuates on the curved shape formed by the curved block a21 and the semicircular block a22 in the rotating ring a2, the liquid generates an inward force under the rotating force, the effect of the rotating force is reduced, the rule of the liquid rotating along the inner wall is damaged through the dislocation of the circulating groove a4, the mutual circulation between the liquid is carried out, the two rotating mechanisms 224 generate dislocation difference, the liquid is circulated back and forth, the two rotating inner walls are divided to reduce the pressure of the liquid, and the cell rupture in the liquid is avoided.
Example 2:
as shown in fig. 6-7:
the 233 is provided with a tilting mechanism w1, a sliding plate w2 and a drainage groove w3, the drainage groove w3 is attached to the inner side of the sliding plate w2, the tilting mechanism w1 is positioned on the inner side of the sliding plate w2, the sliding plate w2 is embedded and fixed inside the fixing groove 21, the inclination angles of the drainage grooves w3 are the same, and the drainage groove w3 is of a continuous bending structure, so that liquid moves upwards along with the drainage groove w3 under the action of rotating force, and the horizontal position of the liquid is changed.
The tilting mechanism w1 is provided with a sealing groove w11, a stress block w12, a rubber plate w13 and a fixing frame w14, the sealing groove w11 is located on the right side of the rubber plate w13, the stress block w12 is attached to the left side of the fixing frame w14, the rubber plate w13 is embedded in the left side of the fixing frame w14, the fixing frame w14 is installed on the inner side of the sliding plate w2, the sealing groove w11 is of a tilting structure with a wider upper end and a narrower lower end, the stress block w12 is attached to the right side of the rubber plate w13, liquid moves outwards under the rotating force, and according to the mechanical principle, the liquid moves upwards along the tilted sealing groove w11 and compresses the sealing groove w11, so that the stress block w12 is subjected to pressure, the liquid on the inner side of the rubber plate w13 fluctuates, and the fluctuation generates upward pressure under the rotating force, thereby driving the liquid to stir upwards and improving.
The embodiment has the following specific use modes and functions:
in the invention, when liquid flows on the inner wall of the placing tank 23, the liquid moves upwards along the drainage groove w3 on the inner side of the sliding plate w2 under the action of rotating force, so that the horizontal position of the liquid is changed, the liquid moves outwards under the action of rotating force, when the liquid enters the tilting mechanism w1, the liquid expands outwards according to the mechanical principle, and moves upwards along the tilted sealing groove w11 under the action of rotating force to compress the sealing groove w11, so that the stress block w12 is stressed, so that the liquid on the inner side of the rubber plate w13 fluctuates, and the fluctuation generates upward pressure under the action of rotating force, so that the liquid is driven to be stirred upwards, and the mixing degree of the liquid is.
The technical scheme of the invention is utilized, or a similar technical scheme is designed by a person skilled in the art based on the inspiration of the technical scheme of the invention, so that the technical effects are achieved, and the technical scheme and the method fall into the protection scope of the invention.
Claims (6)
1. The utility model provides an agitator for plant cell culture device, its structure includes motor (1), rabbling mechanism (2), fixed plate (3), controller (4), lifter plate (5), motor (1) inlays admittedly in rabbling mechanism (2) upper end, install in lifter plate (5) side the upper end of rabbling mechanism (2), install in lifter plate (5) side controller (4), the lower extreme welding of rabbling mechanism (2) is at controller (4) upper surface, lifter plate (5) are installed in fixed plate (3) upper end, its characterized in that:
rabbling mechanism (2) are equipped with fixed slot (21), agitating unit (22), place jar (23), fixer (24), block lid (25), agitating unit (22) are installed in fixer (24) lower extreme, agitating unit (22) are located and place jar (23) inside, block lid (25) and place jar (23) screw-thread fit, the lower extreme of placing jar (23) is inlayed admittedly in fixed slot (21) inside, install in the side of lifter plate (5) fixer (24).
2. An agitator for a plant cell culture apparatus according to claim 1, wherein: agitating unit (22) are equipped with axis of rotation (221), connecting rod (222), dwang (223), rotary mechanism (224), connecting rod (222) are inlayed and are fixed in axis of rotation (221) outside, dwang (223) are installed in axis of rotation (221) outside, dwang (223) are located rotary mechanism (224) inboard, axis of rotation (221) are inlayed and are fixed at fixer (24) lower extreme.
3. An agitator for a plant cell culture apparatus according to claim 2, wherein: the rotating mechanism (224) is provided with a flow hole (a1), a rotating ring (a2), a fixing groove (a3), a flow groove (a4) and a connecting frame (a5), wherein the rotating ring (a2) is embedded in the middle of the connecting frame (a5), the flow hole (a1) and the rotating ring (a2) are of an integrated structure, the flow groove (a4) is located on the inner side of the rotating ring (a2), the fixing groove (a3) is located on the inner side of the rotating ring (a2), and the fixing groove (a3) is embedded on the outer side of the rotating rod (223).
4. An agitator for a plant cell culture apparatus according to claim 3, wherein: the rotating ring (a2) is provided with a curved block (a21), a semi-circular block (a22) and a circular ring (a23), the curved block (a21) and the semi-circular block (a22) are of an integrated structure, the semi-circular block (a22) is attached to two sides of the circular ring (a23), and the circular ring (a23) is located in the middle of the connecting frame (a 5).
5. An agitator for a plant cell culture apparatus according to claim 1, wherein: the (233) is provided with a tilting mechanism (w1), a sliding plate (w2) and a drainage groove (w3), the drainage groove (w3) is attached to the inner side of the sliding plate (w2), the tilting mechanism (w1) is located on the inner side of the sliding plate (w2), and the sliding plate (w2) is embedded in the fixing groove (21).
6. An agitator for a plant cell culture apparatus according to claim 5, wherein: tilting mechanism (w1) is equipped with seal groove (w11), atress piece (w12), rubber slab (w13), mount (w14), seal groove (w11) are located rubber slab (w13) right side, atress piece (w12) laminating is on mount (w14) left side, rubber slab (w13) are embedded and are fixed in mount (w14) left side, mount (w14) are installed in sliding plate (w2) inboard.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011072235.3A CN112266872A (en) | 2020-10-09 | 2020-10-09 | A agitator for plant cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011072235.3A CN112266872A (en) | 2020-10-09 | 2020-10-09 | A agitator for plant cell culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112266872A true CN112266872A (en) | 2021-01-26 |
Family
ID=74337363
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011072235.3A Withdrawn CN112266872A (en) | 2020-10-09 | 2020-10-09 | A agitator for plant cell culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112266872A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113376320A (en) * | 2021-04-28 | 2021-09-10 | 王静 | Biological detection card casket |
| CN115025668A (en) * | 2022-06-15 | 2022-09-09 | 黑龙江省八达路桥建设有限公司 | High-speed asphalt stirring device capable of moving through guide rail |
-
2020
- 2020-10-09 CN CN202011072235.3A patent/CN112266872A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113376320A (en) * | 2021-04-28 | 2021-09-10 | 王静 | Biological detection card casket |
| CN113376320B (en) * | 2021-04-28 | 2023-12-05 | 吉林夏兰生物科技有限公司 | Biological detection cassette |
| CN115025668A (en) * | 2022-06-15 | 2022-09-09 | 黑龙江省八达路桥建设有限公司 | High-speed asphalt stirring device capable of moving through guide rail |
| CN115025668B (en) * | 2022-06-15 | 2023-10-27 | 黑龙江省八达路桥建设有限公司 | High-speed asphalt stirring device capable of moving through guide rail |
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Application publication date: 20210126 |
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| WW01 | Invention patent application withdrawn after publication |