CN112255221A - Colorimetric identification method for concentration and spraying effect of biological protection filter medium - Google Patents
Colorimetric identification method for concentration and spraying effect of biological protection filter medium Download PDFInfo
- Publication number
- CN112255221A CN112255221A CN202011113027.3A CN202011113027A CN112255221A CN 112255221 A CN112255221 A CN 112255221A CN 202011113027 A CN202011113027 A CN 202011113027A CN 112255221 A CN112255221 A CN 112255221A
- Authority
- CN
- China
- Prior art keywords
- filter medium
- polylysine
- trypan blue
- concentration
- fiber layer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/775—Indicator and selective membrane
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a method for indicating the concentration of a biological protection filter medium on the surface of an adsorption fiber layer and the spraying effect by trypan blue color development according to the interaction principle of trypan blue and polylysine. The invention has the advantages that in the process of producing the novel protective article taking the biological protective filter medium as the main filter material, the spraying effect and the effectiveness of the filter material can be judged by visual observation without any instrument, the detection cost is low, and the operation is simple.
Description
Technical Field
The invention relates to a colorimetric identification method for the spraying effect of a biological protection filter medium, in particular to a method for rapidly identifying the concentration and the spraying effect of the biological protection filter medium on the surface of an adsorption fiber layer by trypan blue color development in a mode of detecting polylysine by trypan blue.
Background
With the increasing self-protection awareness, people have higher and higher demands on individual protective articles, and the types and functions of the protective articles are continuously developed. Different protective media have different design requirements, quality evaluation indexes and methods according to specific users and use environments. The biological protection filter medium has wide market prospect as a protection medium which can effectively filter small particle pollutants and can prevent viruses, allergens and bacteria. The invention relates to a biological protection filter medium and an application technology thereof, which are a novel protection article prepared by spraying the biological protection filter medium on the surface of an adsorption fiber layer, wherein the biological protection filter medium and the application technology thereof have already been obtained by the national invention patent (application number: 201110145600.3). In the production process of the novel protective product, the adhesion and spraying effects of the biological protective filter medium on the surface of the adsorption fiber layer have direct influence on the protective efficiency and effectiveness. Therefore, a method for rapidly and effectively identifying the concentration of the biological protection filter medium on the surface of the adsorption fiber layer and the spraying effect is needed to be found.
Disclosure of Invention
Polylysine is one of its major components in the preparation of a bioprotective filter medium. Polylysine can help cells and proteins attach to solid surfaces. Meanwhile, polylysine has positive charges and is a positive charge enhancer, and can enhance the electrostatic interaction between negative ions of cell membranes and positive ions of culture surface attachment factors. Trypan blue, also called trypan blue, is a cell reactive dye commonly used to detect the integrity of cell membranes and to detect whether cells are viable. Its aqueous solution is blue, has the property that its absorptivity is maximum at lmax 580nm, and has the estimated epsilon 580 of molar absorption coefficient spectrum 2.0104M-1cm-1The area of (a). Another important property of trypan blue is that it carries a large negative charge under acidic conditions.
Because polylysine has a large amount of positive charges, trypan blue solution under an acidic condition has negative charges, polylysine with positive charges and trypan blue with negative charges are combined to form blue precipitates, whether adequate polylysine molecules are adsorbed on the adsorption fiber layer or not can be identified according to the color depth of the precipitates, and the concentration of the biological protection filter medium can be judged according to the sufficient polylysine molecules adsorbed on the adsorption fiber layer. According to the principle, the invention provides a method for rapidly and efficiently identifying the surface concentration and the spraying effect of the biological protection filter medium on the adsorption fiber layer. The method is characterized in that the concentration and the spraying effect of the biological protection filter medium on the surface of the adsorption fiber layer are rapidly identified by trypan blue color development by using a mode of detecting polylysine by trypan blue.
The invention adopts the following technical scheme:
a method for rapidly identifying the concentration of a biological protection filter medium on the surface of an adsorption fiber layer and the spraying effect by trypan blue color development is characterized in that trypan blue detection liquid is dripped on an adsorption fiber layer sample solidified with the biological protection filter medium, the adsorption fiber layer is washed after incubation until the color is stable, then the adsorption fiber layer is compared with a colorimetric standard series, the concentration of polylysine on the adsorption fiber layer is identified by adopting a visual colorimetry, and the concentration of the biological protection filter medium and the spraying effect are identified by combining the specific gravity of polylysine and other components in the biological protection filter medium.
The preparation steps of the colorimetric standard series are as follows:
(1) dropwise adding trypan blue detection liquid on a known adsorption fiber layer sample of a biological protection filter medium solidified with different polylysine concentrations, and washing the adsorption fiber layer after incubation until the color is stable;
(2) measuring absorbance at the maximum absorption wavelength by using an ultraviolet-visible spectrophotometer;
(3) drawing a standard curve and obtaining a standard curve equation by taking the absorbance as a vertical coordinate and the concentration of polylysine as a horizontal coordinate;
(4) a colorimetric standard series was made that indicated different polylysine concentrations according to the standard curve equation.
The preparation method of the trypan blue detection solution comprises the following steps: commercially available trypan blue solution with a concentration of 0.4%, before staining in trypan blue solution: hydrochloric acid 10: 1 equivalent of hydrochloric acid is added into the mixture according to the proportion of 1, the mixture is fully mixed, and the pH value is adjusted to 5.0.
The bioprotective filtration media contains at least one polylysine.
The specific gravity of polylysine and other components in the biological protective filter medium is known.
The biological protection filter medium is solidified on the adsorption fiber layer by adopting a high-pressure spraying method or a soaking method.
The original color of the adsorption fiber layer is white.
The invention has the following remarkable advantages:
the invention indicates the concentration and the spraying effect of the biological protection filter medium on the surface of the adsorption fiber layer by trypan blue color development according to the interaction principle of trypan blue and polylysine, and has the advantages that in the production process of the novel protection product taking the biological protection filter medium as the main filter material, the spraying effect and the effectiveness of the filter material can be judged by visual observation without any instrument, the detection cost is low, and the operation is simple. Meanwhile, the invention can directly carry out semi-quantitative analysis on the concentration of the biological protection filter medium on the adsorption fiber layer by comparing with colorimetric standard series, and when the concentration of the biological protection filter medium has more accurate requirement, the invention can also utilize the ultraviolet-spectrophotometry to measure the absorbance at the maximum wavelength and substitute the absorbance into a standard curve equation to carry out quantitative analysis, and the flexibility can meet different requirements in the production process.
Drawings
FIG. 1 is a standard curve plotted with polylysine concentration on the abscissa and absorbance of OD580nm on the ordinate in accordance with an example of the present invention.
Detailed Description
The preparation method of the trypan blue detection solution comprises the following steps: commercially available trypan blue solution with a concentration of 0.4%, before staining in trypan blue solution: hydrochloric acid 10: 1 equivalent of hydrochloric acid is added into the mixture according to the proportion of 1, the mixture is fully mixed, and the pH value is adjusted to 5.0.
And (3) identifying the surface concentration and the spraying effect of the biological protection filter medium on the adsorption fiber layer: the trypan blue detection solution is dripped on an adsorption fiber layer sample solidified with the biological protection filter medium, the adsorption fiber layer is washed after incubation until the color is stable, then the trypan blue detection solution is compared with a colorimetric standard series, the concentration of polylysine on the adsorption fiber layer is identified by adopting a visual colorimetric method, and the concentration and the spraying effect of the biological protection filter medium are identified by combining the specific gravity of the polylysine and other components in the biological protection filter medium.
Colorimetric standard series: the preparation steps of the colorimetric standard series are as follows:
(1) dropwise adding trypan blue detection liquid on a known adsorption fiber layer sample of a biological protection filter medium solidified with different polylysine concentrations, and washing the adsorption fiber layer after incubation until the color is stable;
(2) measuring absorbance at the maximum absorption wavelength by using an ultraviolet-visible spectrophotometer;
(3) drawing a standard curve and obtaining a standard curve equation by taking the absorbance as a vertical coordinate and the concentration of polylysine as a horizontal coordinate;
(4) a colorimetric standard series was made that indicated different polylysine concentrations according to the standard curve equation.
The invention is further illustrated by the following examples:
[ example 1 ]
Preparing trypan blue detection solution: a0.4% Trypan blue solution was prepared, the buffer of which was phosphate buffered saline and the pH was 7.2. A diluted hydrochloric acid solution was prepared with a concentration of 1N and a pH < 3.0. And adding 1000 microliters of hydrochloric acid into 10 milliliters of trypan blue solution, fully and uniformly mixing, and adjusting the pH value to 5.0 to obtain the trypan blue detection solution.
And (3) identifying the surface concentration and the spraying effect of the biological protection filter medium on the surface of the adsorption fiber layer, namely dripping 1 ml of prepared trypan blue detection solution on a white non-woven fabric sample sprayed with the biological protection filter medium, wherein the solidified biological protection filter medium on the non-woven fabric only contains polylysine which is a specific binding receptor. Incubation for 10 minutes at room temperature resulted in a significant color change. And washing the discolored non-woven fabric twice until the color is stable. According to the color shade after washing, the polylysine concentration on the non-woven fabric can be semi-quantitatively analyzed by a visual colorimetry according to a contrast colorimetric standard series, or the absorbance is measured at the wavelength of 580nm by an ultraviolet-spectrophotometry method and is substituted into a standard curve equation to perform quantitative analysis. Because only one specific binding receptor of the polylysine exists in the biological filtration protection medium, the concentration of the polylysine is the concentration of the biological protection medium.
Colorimetric standard series: 1 ml of prepared trypan blue detection solution is respectively dripped on 6 white non-woven fabrics sprayed by biological filtration protective media with different polylysine concentrations. The concentration of polylysine in the biological protection filter medium is as follows in sequence: 0g/ml, 6g/ml, 12g/ml, 24g/ml, 48g/ml and 96 g/ml. And incubating for 10 minutes at room temperature to generate an obvious color change method, and washing the discolored non-woven fabric twice until the color is stable. And (3) respectively measuring absorbance at the wavelength of 580nm by adopting an ultraviolet-visible spectrophotometer, drawing a standard curve by taking the absorbance as a vertical coordinate and the concentration of the polylysine as a horizontal coordinate to obtain a standard curve equation, and then manufacturing color development strips indicating different polylysine concentrations according to the standard curve equation to serve as a colorimetric standard series.
[ example 2 ]
Preparing trypan blue detection solution: a0.4% Trypan blue solution was prepared, the buffer of which was phosphate buffered saline and the pH was 7.2. A diluted hydrochloric acid solution was prepared with a concentration of 1N and a pH < 3.0. And adding 1000 microliters of hydrochloric acid into 10 milliliters of trypan blue solution, fully and uniformly mixing, and adjusting the pH value to 5.0 to obtain the trypan blue detection solution.
And (3) identifying the surface concentration and spraying effect of the biological protection filter medium on the surface of the adsorption fiber layer, namely dripping 1 ml of prepared trypan blue detection solution on a white HEPA filter membrane sample sprayed with the biological protection filter medium, wherein the biological protection filter medium solidified on the HEPA filter membrane contains two specific receptors of polylysine and sialic acid, and the weight ratio of the polylysine to the sialic acid is 1: 2. incubation for 10 minutes at room temperature resulted in a significant color change. Washing the discolored HEPA filtering membrane twice until the color is stable. According to the color shade after washing, the polylysine concentration on the HEPA filter membrane is semi-quantitatively analyzed by a visual colorimetry according to a contrast colorimetric standard series, or the absorbance is measured at the wavelength of 580nm by an ultraviolet-spectrophotometry method and is substituted into a standard curve equation for quantitative analysis. According to the weight ratio of polylysine to sialic acid in the biological filtration protection medium of 1: and 2, the polylysine concentration x3 is the concentration of the biological protection filter medium.
Colorimetric standard series: 1 ml of prepared trypan blue detection solution is respectively dripped on 6 white HEPA filter membranes sprayed and treated by biological filter protective media with different polylysine concentrations. The concentration of polylysine in the biological protection filter medium is as follows in sequence: 0g/ml, 6g/ml, 12g/ml, 24g/ml, 48g/ml and 96 g/ml. And incubating for 10 minutes at room temperature to generate an obvious color change method, and washing the color-changed HEPA filtering membrane twice until the color is stable. And (3) respectively measuring absorbance at the wavelength of 580nm by adopting an ultraviolet-visible spectrophotometer, drawing a standard curve by taking the absorbance as a vertical coordinate and the concentration of the polylysine as a horizontal coordinate to obtain a standard curve equation, and then manufacturing color development strips indicating different polylysine concentrations according to the standard curve equation to serve as a colorimetric standard series.
[ example 3 ]
Preparing trypan blue detection solution: preparing trypan blue detection solution: a0.4% Trypan blue solution was prepared, the buffer was phosphate buffered saline, and the pH was 7.2. A diluted hydrochloric acid solution was prepared with a concentration of 1N and a pH < 3.0. And adding 1000 microliters of hydrochloric acid into 10 milliliters of trypan blue solution, fully and uniformly mixing, and adjusting the pH value to 5.0 to obtain the trypan blue detection solution.
And (3) identifying the surface concentration and the spraying effect of the biological protection filter medium on the surface of the adsorption fiber layer, namely dripping 1 ml of prepared trypan blue detection solution on a white non-woven fabric sample sprayed with the biological protection filter medium, wherein the biological protection filter medium solidified on the non-woven fabric contains three specific receptors of polylysine, sialic acid and polymyxin B, and the weight ratio of the receptors is 1: 2: 1. incubation for 10 minutes at room temperature resulted in a significant color change. And washing the discolored non-woven fabric twice until the color is stable. According to the color shade after washing, the polylysine concentration on the non-woven fabric can be semi-quantitatively analyzed by a visual colorimetry according to a contrast colorimetric standard series, or the absorbance is measured at the wavelength of 580nm by an ultraviolet-spectrophotometry method and is substituted into a standard curve equation to perform quantitative analysis. According to the weight ratio of polylysine, sialic acid and polymyxin in the biological filtration protection medium of 1: 2: and 1, the polylysine concentration x4 is the concentration of the biological protection filter medium.
Colorimetric standard series: 1 ml of prepared trypan blue detection solution is respectively dripped on 6 white non-woven fabrics sprayed by biological filtration protective media with different polylysine concentrations. The concentration of polylysine in the biological protection filter medium is as follows in sequence: 0g/ml, 6g/ml, 12g/ml, 24g/ml, 48g/ml and 96 g/ml. And incubating for 10 minutes at room temperature to generate an obvious color change method, and washing the discolored non-woven fabric twice until the color is stable. And (3) respectively measuring absorbance at the wavelength of 580nm by adopting an ultraviolet-visible spectrophotometer, drawing a standard curve by taking the absorbance as a vertical coordinate and the concentration of the polylysine as a horizontal coordinate to obtain a standard curve equation, and then manufacturing color development strips indicating different polylysine concentrations according to the standard curve equation to serve as a colorimetric standard series.
After many experiments and preferably, the mixture is stained with 0.4% trypan blue dye solution before staining with the trypan blue solution: hydrochloric acid 10: 1 equivalent of hydrochloric acid is added into the mixture according to the proportion of 1, the mixture is fully mixed, the pH value is adjusted to 5.0, so that the detection sensitivity is obviously increased, and the standard trypan blue detection solution is obtained. The trypan blue detection liquid is used for detecting the non-woven fabric (also can be a gauze or HEPA filter membrane or other adsorption fiber materials) sprayed and treated by the biological protection filter medium, and the result shows that the color of the non-woven fabric is gradually deepened from colorless to light blue along with the increase of the concentration of the polylysine in the biological protection filter medium, and the depth of the coloring is in direct proportion to the concentration of the polylysine in the biological protection filter medium adhered to the non-woven fabric. The good corresponding relation is that y is 0.44x-0.44, R2 is 0.9994, as long as blue is displayed, the attachment of the biological protection filter medium on the non-woven fabric is shown, the deeper the blue is, the more molecules are attached on the non-woven fabric by the biological protection filter medium, the higher the concentration is, and the better the protection effect is. Meanwhile, the invention can directly carry out semi-quantitative analysis on the concentration of the biological protection filter medium on the adsorption fiber layer by comparing with colorimetric standard series, and when the concentration of the biological protection filter medium has more accurate requirement, the invention can also utilize the ultraviolet-spectrophotometry to measure the absorbance at the maximum wavelength and substitute the absorbance into a standard curve equation to carry out quantitative analysis, and the flexibility can meet different requirements in the production process.
The above description is only a preferred embodiment of the present invention, and all equivalent changes and modifications made in accordance with the claims of the present invention should be covered by the present invention.
Claims (7)
1. A method for rapidly identifying the concentration and the spraying effect of a biological protection filter medium on the surface of an adsorption fiber layer by trypan blue color development in a mode of detecting polylysine by trypan blue. The method is characterized in that trypan blue detection liquid is dripped on an adsorption fiber layer sample solidified with the biological protection filter medium, the adsorption fiber layer is washed by water after incubation until the color is stable, then the adsorption fiber layer is compared with a colorimetric standard series, the concentration of polylysine on the adsorption fiber layer is identified by adopting a visual colorimetric method, and the concentration and the spraying effect of the biological protection filter medium are identified by combining the specific gravity of the polylysine and other components in the biological protection filter medium.
2. The method of claim 1, wherein the colorimetric standard series is prepared by:
(1) dropwise adding trypan blue detection liquid on a known adsorption fiber layer sample of a biological protection filter medium solidified with different polylysine concentrations, and washing the adsorption fiber layer after incubation until the color is stable;
(2) measuring absorbance at the maximum absorption wavelength by using an ultraviolet-visible spectrophotometer;
(3) drawing a standard curve and obtaining a standard curve equation by taking the absorbance as a vertical coordinate and the concentration of polylysine as a horizontal coordinate;
(4) a colorimetric standard series was made that indicated different polylysine concentrations according to the standard curve equation.
3. The method according to any one of claims 1 to 2, wherein the trypan blue detection solution is prepared by a method comprising: trypan blue solution at concentration 0.4%, before staining as trypan blue solution: hydrochloric acid 10: 1 equivalent of hydrochloric acid is added into the mixture according to the proportion of 1, the mixture is fully mixed, and the pH value is adjusted to 5.0, so that trypan blue detection liquid is obtained.
4. The method of any one of claims 1-2, wherein the biocontainment filter medium comprises at least one polylysine.
5. A method according to any one of claims 1 to 2 wherein the specific gravity of polylysine to the other components of the biocontained filter medium is known.
6. The method of any of claims 1-2, wherein the biocontainment filter medium is cured to the adsorbent fiber layer by high pressure spraying or soaking.
7. The method according to any one of claims 1-2, wherein the adsorbent fiber layer is white in its original color.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011113027.3A CN112255221A (en) | 2020-10-16 | 2020-10-16 | Colorimetric identification method for concentration and spraying effect of biological protection filter medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011113027.3A CN112255221A (en) | 2020-10-16 | 2020-10-16 | Colorimetric identification method for concentration and spraying effect of biological protection filter medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112255221A true CN112255221A (en) | 2021-01-22 |
Family
ID=74244715
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011113027.3A Pending CN112255221A (en) | 2020-10-16 | 2020-10-16 | Colorimetric identification method for concentration and spraying effect of biological protection filter medium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112255221A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1673357A (en) * | 2005-02-03 | 2005-09-28 | 山东大学 | Method for digesting blood smooth muscle |
| CA2686722A1 (en) * | 2007-05-06 | 2008-11-13 | Sloan-Kettering Institute For Cancer Research | Methods for treating and preventing gi syndrome and graft versus host disease |
| CN102302877A (en) * | 2011-06-01 | 2012-01-04 | 李红 | Biological protection filter medium and application thereof |
| EP3373011A1 (en) * | 2015-11-07 | 2018-09-12 | Hunan Skyworld Biotechnologies Co. | Methionine adenosyltransferase biological activity assay method and reagent kit |
-
2020
- 2020-10-16 CN CN202011113027.3A patent/CN112255221A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1673357A (en) * | 2005-02-03 | 2005-09-28 | 山东大学 | Method for digesting blood smooth muscle |
| CA2686722A1 (en) * | 2007-05-06 | 2008-11-13 | Sloan-Kettering Institute For Cancer Research | Methods for treating and preventing gi syndrome and graft versus host disease |
| CN102302877A (en) * | 2011-06-01 | 2012-01-04 | 李红 | Biological protection filter medium and application thereof |
| EP3373011A1 (en) * | 2015-11-07 | 2018-09-12 | Hunan Skyworld Biotechnologies Co. | Methionine adenosyltransferase biological activity assay method and reagent kit |
Non-Patent Citations (1)
| Title |
|---|
| WEI-CHIANG SHEN 等: "Colorimetric determination of microgram quantities of polylysine by trypan blue precipitation", 《ANALYTICAL BIOCHEMISTRY》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5852126A (en) | Polymerisable composition and the use thereof | |
| CN103558189B (en) | Device and method for detection of humidity-compromised urine test strips | |
| CN109253907B (en) | Method for rapidly and auxiliarily detecting micro-plastic in water environment sample by using Nile red dyeing | |
| EP1327874B1 (en) | Sensor formulation for the detection of a gas composition and method for detecting presence of respiring organisms | |
| US4592994A (en) | Method for the determination of biochemical data | |
| DE69910667T2 (en) | Composition for the detection of a microorganism in a sample | |
| CN101203617A (en) | Device for rapid detection and identification of single microorganisms without preliminary growth | |
| CN104946248A (en) | Water-soluble sulphurous acid hydrogen root ratiometric fluorescent probe and application thereof | |
| CA1201963A (en) | Method of concentrating and measuring unicellular organisms | |
| US20040171094A1 (en) | Oxygen sensors disposed on a microtiter plate | |
| CN101464409B (en) | Apparatus and method for fast quantitative bacteria detection | |
| CN206420773U (en) | A kind of portable formaldehyde examination device based on fluorescence probe method | |
| CN112255221A (en) | Colorimetric identification method for concentration and spraying effect of biological protection filter medium | |
| CN102353749A (en) | Test paper for quickly detecting formaldehyde in air | |
| CN107057685B (en) | Europium fluorescence probe and test paper based on biphenyl dicarboxylic acid and the application in detection p-phenylenediamine | |
| CN1828303A (en) | Method for quick detection of microbe | |
| Moreira-Turcq et al. | Chemical and biological characterization of particles by flow cytometry in the Krka estuary, Croatia | |
| CN109884015A (en) | The method of application and detection CHL of the MOF-Zn fluorescent optical sensor in detection chloramphenicol | |
| WO2003036293A1 (en) | Method for simultaneous optical measurement of a ph-value and dissolved oxygen content | |
| DE10251893A1 (en) | Optical sensor, for quantitative measurement of enzyme activities, has a transparent layer on a carrier with a grid between them to switch electromagnetic waves on/off as a disposable chip | |
| CN106153586B (en) | Application of the europium fluorescence probe based on terephthalic acid (TPA) in detection aniline | |
| WO1994017388A1 (en) | Ion selective fluorosensor based on the inner filter effect | |
| CN104459128A (en) | Salmonella immunochromatography test strip based on low-noise excitation type fluorescent mark | |
| CN220795047U (en) | Be used for pesticide waste water biological detection device and microcystis aeruginosa carrier | |
| CN215179676U (en) | Fluorescence excitation type detection pen |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20210122 |