CN112251471A - Enzymatic process for preparing biodiesel from acidified oil - Google Patents
Enzymatic process for preparing biodiesel from acidified oil Download PDFInfo
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- CN112251471A CN112251471A CN202011157859.5A CN202011157859A CN112251471A CN 112251471 A CN112251471 A CN 112251471A CN 202011157859 A CN202011157859 A CN 202011157859A CN 112251471 A CN112251471 A CN 112251471A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
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- C—CHEMISTRY; METALLURGY
- C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
- C10L—FUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G, C10K; LIQUEFIED PETROLEUM GAS; ADDING MATERIALS TO FUELS OR FIRES TO REDUCE SMOKE OR UNDESIRABLE DEPOSITS OR TO FACILITATE SOOT REMOVAL; FIRELIGHTERS
- C10L1/00—Liquid carbonaceous fuels
- C10L1/02—Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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Abstract
The invention provides an enzymatic process for preparing biodiesel from acidified oil, and relates to the technical field of biodiesel preparation. The enzymatic process for preparing the biodiesel from the acidified oil comprises the following materials in parts by weight: 400 portions of raw oil, 40 to 60 portions of biological enzyme catalyst and 40 to 60 portions of methanol, wherein the enzymatic process for preparing the biodiesel from the acidified oil comprises the following components in parts by weight: 500 parts of raw oil, 50 parts of a biological enzyme composite catalyst and 50 parts of methanol. The invention changes the original process of carrying out the liquid enzyme reaction and then carrying out the solid enzyme reaction and then distilling into the process of carrying out the distillation and then carrying out the solid enzyme reaction, improves the reaction speed of the product, can separate other matters such as pigments, colloids, non-fatty acid acidic substances, mechanical impurities and the like in the acidified oil by simultaneously carrying out the distillation, can effectively protect the activity of the immobilized fatty acid, and obviously increases the use times of the enzyme.
Description
Technical Field
The invention relates to the technical field of biodiesel preparation, in particular to an enzymatic process for preparing biodiesel from acidified oil.
Background
Biodiesel is one kind of biomass energy, and is a monoalkyl ester of long-chain fatty acid obtained by biological utilization of thermal cracking and other technologies. Biodiesel is a mixture of complex organic constituents with very high oxygen content, which are mainly some organics with large molecular weight, including almost all kinds of oxygenated organics. The biodiesel basically contains no sulfur and aromatic hydrocarbon, has high cetane number, can be biodegraded, is non-toxic and harmless to the environment. Its high oxygen content CN value is favorable to normal combustion of compression ignition machine so as to reduce discharge of harmful substances in tail gas, so that it is called low-pollution fuel.
The production method of the biodiesel mainly comprises a chemical method, a biological enzyme method and a supercritical alcohol method. In the existing production method, when biodiesel production is carried out on oils with high content of colloid and pigment such as vegetable oil, acid soap foot acidified oil, white clay oil and acidified oil in a biodiesel factory, an enzymatic process generally comprises a process of carrying out a solid enzyme reaction and then distilling on the acidified oil after a liquid enzyme reaction, but the process of carrying out the solid enzyme reaction and then distilling is carried out on the acidified oil, a carrier of immobilized lipase in a catalyst of the solid enzyme reaction can adsorb pigment and colloid to influence the activity of the immobilized lipase, the solid enzyme reaction is a reaction of fatty acid and methanol to generate fatty acid methyl ester and water, the national standard requirement that the acid value of a product is lower than 0.5mgKOH/g is to carry out online dehydration in the reaction process and in the middle, the reaction liquid after the liquid enzyme reaction contains 0.5-1% of water, and the water generated by the reaction is 0.3-0.4%, so the reaction speed is lower.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides an enzymatic process for preparing biodiesel from acidified oil, which solves the problems that in the prior art, the solid enzyme reaction speed is low, and the activity of immobilized lipase is easily influenced.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: the enzymatic process for preparing the biodiesel from the acidified oil comprises the following materials in parts by weight: 400 portions of raw oil, 600 portions of bio-enzyme catalyst, 40 to 60 portions of methanol.
Preferably, the enzymatic process for preparing the biodiesel from the acidified oil comprises the following components in parts by weight: 500 parts of raw oil, 50 parts of a biological enzyme composite catalyst and 50 parts of methanol.
An enzymatic process for preparing biodiesel from acidified oil comprises the following process steps:
s1, carrying out liquid enzyme reaction on the raw oil according to the original processing mode in the prior art to obtain liquid A;
s2, performing centrifugal separation on the solution A by using a horizontal screw centrifuge, wherein the rotation speed of the horizontal screw centrifuge is 10000-13000 rmp, and the centrifugation time is 20-30 min, so as to remove part of solid impurities in the solution A;
s3, pumping the liquid A after the centrifugal separation in the step 2 into a distillation retort by an oil pump, heating the liquid A to 400 ℃ to gasify the liquid A, condensing the gasified liquid A into liquid by a condenser, and separating impurities such as pigment, colloid, waste fatty acid substances, mechanical impurities and the like in the distilled liquid A to obtain liquid B;
and S4, pumping the liquid B into a reaction kettle by an oil pump, heating to raise the temperature of the liquid B in the reaction kettle No. 2 to 60-80 ℃, adding a biological enzyme catalyst and methanol, performing a circulating reaction for 4-6 hours, and filtering by a plate frame after the reaction is finished to obtain the biodiesel.
Preferably, the stirring kettle in the step 4 is stirred for 5-10 min at the temperature of 60-80 ℃, the stirring speed is 2000-3000 r/min, and the heat preservation time is 20-60 min.
Preferably, the lipase is a complex immobilized enzyme of one or more of the lipases produced by bacteria, fungi, yeast or animal pancreas.
Preferably, the raw oil is vegetable oil, acid soap keratin acid oil, white clay oil, biodiesel acid oil and other oils with high content of colloid and pigment.
(III) advantageous effects
The invention provides an enzymatic process for preparing biodiesel from acidified oil. The method has the following beneficial effects:
the invention changes the original process of carrying out the liquid enzyme reaction and then carrying out the solid enzyme reaction and then distilling into the process of carrying out the distillation and then carrying out the solid enzyme reaction. In order to ensure that the acid value of the product is lower than the national standard requirement of 0.5mgKOH/g, the product needs to be dehydrated on line in the reaction process and the middle, the reaction liquid after the liquid enzyme reaction contains 0.5 to 1 percent of water and 0.3 to 0.4 percent of water generated by the reaction, the water can be dehydrated to about 0.05 percent by distillation, so that only 0.3 to 0.4 percent of water generated by the reaction is removed during the reaction on-line dehydration, and the reaction speed is nearly doubled; meanwhile, other substances such as pigments, colloid, non-fatty acid acidic substances, mechanical impurities and the like in the acidified oil can be separated by distillation, so that the activity of the immobilized fatty acid can be effectively protected, and the use frequency of the enzyme is obviously increased.
Detailed Description
The following will clearly and completely describe the technical solutions in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows:
the embodiment of the invention provides an enzymatic process for preparing biodiesel from acidified oil, which comprises the following components in parts by weight: 500 parts of raw oil, 50 parts of a biological enzyme composite catalyst and 50 parts of methanol.
An enzymatic process for preparing biodiesel from acidified oil comprises the following process steps:
s1, carrying out liquid enzyme reaction on the raw oil according to the original processing mode in the prior art to obtain liquid A;
s2, performing centrifugal separation on the solution A by using a horizontal screw centrifuge, wherein the rotation speed of the horizontal screw centrifuge is 10000-13000 rmp, and the centrifugation time is 20-30 min, so as to remove part of solid impurities in the solution A;
s3, pumping the liquid A after the centrifugal separation in the step 2 into a distillation retort by an oil pump, heating the liquid A to 400 ℃ to gasify the liquid A, condensing the gasified liquid A into liquid by a condenser, and separating impurities such as pigment, colloid, waste fatty acid substances, mechanical impurities and the like in the distilled liquid A to obtain liquid B;
and S4, pumping the liquid B into a reaction kettle by an oil pump, heating to raise the temperature of the liquid B in the reaction kettle No. 2 to 60-80 ℃, adding a biological enzyme catalyst and methanol, performing a circulating reaction for 4-6 hours, and filtering by a plate frame after the reaction is finished to obtain the biodiesel.
Example two:
the embodiment of the invention provides an enzymatic process for preparing biodiesel from acidified oil, which comprises the following components in parts by weight: 400 parts of raw oil, 40 parts of a biological enzyme composite catalyst and 40 parts of methanol.
An enzymatic process for preparing biodiesel from acidified oil comprises the following process steps:
s1, carrying out liquid enzyme reaction on the raw oil according to the original processing mode in the prior art to obtain liquid A;
s2, performing centrifugal separation on the solution A by using a horizontal screw centrifuge, wherein the rotation speed of the horizontal screw centrifuge is 10000-13000 rmp, and the centrifugation time is 20-30 min, so as to remove part of solid impurities in the solution A;
s3, pumping the liquid A after the centrifugal separation in the step 2 into a distillation retort by an oil pump, heating the liquid A to 400 ℃ to gasify the liquid A, condensing the gasified liquid A into liquid by a condenser, and separating impurities such as pigment, colloid, waste fatty acid substances, mechanical impurities and the like in the distilled liquid A to obtain liquid B;
and S4, pumping the liquid B into a reaction kettle by an oil pump, heating to raise the temperature of the liquid B in the reaction kettle No. 2 to 60-80 ℃, adding a biological enzyme catalyst and methanol, performing a circulating reaction for 4-6 hours, and filtering by a plate frame after the reaction is finished to obtain the biodiesel.
Example three:
the embodiment of the invention provides an enzymatic process for preparing biodiesel from acidified oil, which comprises the following components in parts by weight: 600 parts of raw oil, 60 parts of a biological enzyme composite catalyst and 60 parts of methanol.
An enzymatic process for preparing biodiesel from acidified oil comprises the following process steps:
s1, carrying out liquid enzyme reaction on the raw oil according to the original processing mode in the prior art to obtain liquid A;
s2, performing centrifugal separation on the solution A by using a horizontal screw centrifuge, wherein the rotation speed of the horizontal screw centrifuge is 10000-13000 rmp, and the centrifugation time is 20-30 min, so as to remove part of solid impurities in the solution A;
s3, pumping the liquid A after the centrifugal separation in the step 2 into a distillation retort by an oil pump, heating the liquid A to 400 ℃ to gasify the liquid A, condensing the gasified liquid A into liquid by a condenser, and separating impurities such as pigment, colloid, waste fatty acid substances, mechanical impurities and the like in the distilled liquid A to obtain liquid B;
and S4, pumping the liquid B into a reaction kettle by an oil pump, heating to raise the temperature of the liquid B in the reaction kettle No. 2 to 60-80 ℃, adding a biological enzyme catalyst and methanol, performing a circulating reaction for 4-6 hours, and filtering by a plate frame after the reaction is finished to obtain the biodiesel.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. An enzymatic process for preparing biodiesel from acidified oil is characterized in that: the enzymatic process for preparing the biodiesel from the acidified oil comprises the following materials in parts by weight: 400 portions of raw oil, 600 portions of bio-enzyme catalyst, 40 to 60 portions of methanol.
2. The enzymatic process for preparing biodiesel from acidified oil according to claim 1, wherein the enzymatic process comprises the following steps: the enzymatic process for preparing the biodiesel from the acidified oil comprises the following components in parts by weight: 500 parts of raw oil, 50 parts of a biological enzyme composite catalyst and 50 parts of methanol.
3. A preparation method of an enzymatic process for preparing biodiesel from acidified oil is characterized by comprising the following steps: the method comprises the following enzymatic process steps:
s1, carrying out liquid enzyme reaction on the raw oil according to the original processing mode in the prior art to obtain liquid A;
s2, performing centrifugal separation on the solution A by using a horizontal screw centrifuge, wherein the rotation speed of the horizontal screw centrifuge is 10000-13000 rmp, and the centrifugation time is 20-30 min, so as to remove part of solid impurities in the solution A;
s3, pumping the liquid A after the centrifugal separation in the step 2 into a distillation retort by an oil pump, heating the liquid A to 400 ℃ to gasify the liquid A, condensing the gasified liquid A into liquid by a condenser, and separating impurities such as pigment, colloid, waste fatty acid substances, mechanical impurities and the like in the distilled liquid A to obtain liquid B;
and S4, pumping the liquid B into a reaction kettle by an oil pump, heating to raise the temperature of the liquid B in the reaction kettle No. 2 to 60-80 ℃, adding a biological enzyme catalyst and methanol, performing a circulating reaction for 4-6 hours, and filtering by a plate frame after the reaction is finished to obtain the biodiesel.
4. The enzymatic process for preparing biodiesel from acidified oil according to claim 3, wherein the enzymatic process comprises the following steps: in the step 4, the stirring kettle is stirred for 5-10 min at the temperature of 60-80 ℃, the stirring speed is 2000-3000 r/min, and the heat preservation time is 20-60 min.
5. The enzymatic process for preparing biodiesel from acidified oil according to claim 1, wherein the enzymatic process comprises the following steps: the biocatalyst is lipase, and the lipase is composite immobilized enzyme of one or more of lipase produced by bacteria, fungi, yeast or animal pancreas.
6. The enzymatic process for preparing biodiesel from acidified oil according to claim 1, wherein the enzymatic process comprises the following steps: the raw oil is vegetable oil, acid soap keratin acid oil, clay oil, biodiesel acid oil and other oils with high colloid and pigment content.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011157859.5A CN112251471A (en) | 2020-10-26 | 2020-10-26 | Enzymatic process for preparing biodiesel from acidified oil |
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| CN202011157859.5A CN112251471A (en) | 2020-10-26 | 2020-10-26 | Enzymatic process for preparing biodiesel from acidified oil |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115386432A (en) * | 2022-07-29 | 2022-11-25 | 湖南诺睿信生物科技有限公司 | System for producing biodiesel by using double enzymes and production method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102021207A (en) * | 2010-11-04 | 2011-04-20 | 清华大学 | On-line dehydration process for preparing biodiesel from renewable grease through catalysis of lipase |
| CN102660386A (en) * | 2012-01-16 | 2012-09-12 | 四川大学 | Method for preparing biodiesel by base catalysis in combination with lipase catalysis |
| CN103459607A (en) * | 2011-03-30 | 2013-12-18 | 诺维信公司 | Esterification process |
-
2020
- 2020-10-26 CN CN202011157859.5A patent/CN112251471A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102021207A (en) * | 2010-11-04 | 2011-04-20 | 清华大学 | On-line dehydration process for preparing biodiesel from renewable grease through catalysis of lipase |
| CN103459607A (en) * | 2011-03-30 | 2013-12-18 | 诺维信公司 | Esterification process |
| CN102660386A (en) * | 2012-01-16 | 2012-09-12 | 四川大学 | Method for preparing biodiesel by base catalysis in combination with lipase catalysis |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115386432A (en) * | 2022-07-29 | 2022-11-25 | 湖南诺睿信生物科技有限公司 | System for producing biodiesel by using double enzymes and production method thereof |
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