CN112251326A - Device and cutter for labeling nucleic acid probe by gel cutting and purifying isotope - Google Patents
Device and cutter for labeling nucleic acid probe by gel cutting and purifying isotope Download PDFInfo
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- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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Abstract
The invention discloses a device for labeling a nucleic acid probe by a gel cutting and purifying isotope, which consists of a base (1) and a baffle (2), wherein an ultraviolet light source (4) is arranged above the base (1), the ultraviolet light source (4) is connected with the base (1) through a bracket (3), and a color developing panel (5) is arranged on the base (1). The color development panel (5) is a fluorescent thin-layer chromatography plate, and can also be a paraffin film. Thus, the DNA bands can be visually positioned by an ultraviolet shadow method, and the method is applied to the process of cutting gel and purifying the isotope labeled nucleic acid probe. The invention also discloses a cutter for cutting the electrophoresis gel, wherein the cutter head is formed by surrounding four blades, so that the electrophoresis gel can be cut more quickly, and the time is saved in the process of purifying the probe.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a method for cutting gel and purifying an isotope labeled nucleic acid probe. The invention also relates to a device for cutting gel and purifying the isotope labeled nucleic acid probe. The invention also relates to a cutter for cutting the electrophoretic glue.
Background
The isotope labeled nucleic acid probe has the characteristics of high sensitivity, no change of the chemical property of a nucleic acid sequence, and capability of detecting a target fragment of picogram level, and is widely used in the field of life science. In use32P or33P in PCR or in vitro transcription of labeled oligonucleotides, shorter product fragments are produced due to hydrolysis and premature translation termination. The higher the specific activity of the probe, the higher the sensitivity.Many assays rely on highly active probes, such as Ribonuclease Protection Assay (RPA). The higher the specific activity of the prepared probe, the easier it is to generate short product fragments, and the lower the product yield. In order to avoid interference on experimental results, the probes are often required to be subjected to gel cutting purification, and short product fragments and other substances such as template DNA, transcriptases, NTPs and the like added in the labeling reaction can be effectively removed.
The domestic invention patent CN106047863B discloses a method for cutting gel and purifying isotope labeled nucleic acid probe. The method comprises the following steps: the method comprises the following steps: preparing a nucleic acid fragment with the same base property and base sequence as the target isotope labeled nucleic acid probe; step two: electrophoretically separating said nucleic acid fragments; step three: locating the position of the nucleic acid fragments on the electrophoresis gel using a conventional staining method; step four: using another piece of electrophoresis gel which is the same as the electrophoresis gel in the second step, and adopting the same conditions as the electrophoresis gel in the second step to electrophoretically separate the target isotope labeled nucleic acid probe; step five: after the time length is the same as that of the second step, stopping electrophoresis, and cutting the gel block at the position determined by the third step on the electrophoresis gel in the fourth step; step six: recovering the isotopically labeled nucleic acid probe in the gel mass.
The invention patent CN106047863B provides a method for purifying isotope-labeled nucleic acid probes by cutting gel with few steps of contacting with isotopes and short isotope operation time. The method reduces the steps and time required to manipulate the isotope by predetermining the specific location of the isotope-labeled nucleic acid probe on the electrophoresis gel after the electrophoresis is completed. The method for cutting gel and purifying isotope labeled nucleic acid probe of the invention has the disadvantages that: the method requires the use of two pieces of electrophoresis gel, requires that the two pieces of electrophoresis gel have the same chemical and physical properties, and requires electrophoresis under the same conditions. In the actual operation process, because two different electrophoresis gels are used and electrophoresis is performed in sequence, the two electrophoresis gels are not easy to have absolutely the same chemical and physical properties and the same electrophoresis conditions. The result is that the position of the nucleic acid bands on the two pieces of electrophoresis gel is shifted, thereby reducing the success rate of the method for gel cutting and probe purification.
Disclosure of Invention
In order to overcome the defects of the method for gel cutting and purifying the isotope labeled nucleic acid probe, the invention provides a method for gel cutting and purifying the isotope labeled nucleic acid probe, which can be completed by only using one piece of electrophoresis gel and one-time electrophoresis.
Accordingly, another object of the present invention is to provide an apparatus for purifying isotope-labeled nucleic acid probes by gel cutting using a UV shading method to show DNA bands.
In addition, the technical problem to be solved by the invention is to provide a cutter for quickly cutting electrophoresis gel.
In order to solve the above-mentioned technical problems, the method of the present invention for gel cutting purification of an isotope-labeled nucleic acid probe comprises the steps of:
the method comprises the following steps: preparing a nucleic acid fragment with the same base property and base sequence as the target isotope labeled nucleic acid probe;
step two: electrophoretically separating the nucleic acid fragments from the target isotope-labeled nucleic acid probe;
step three: using an ultraviolet shadow method to locate the positions of the nucleic acid fragments on the electrophoresis gel;
step four: cutting the gel block at the position corresponding to the probe lane, and recovering the target isotope labeled nucleic acid probe.
The target isotope-labeled nucleic acid probe may be any of single-stranded DNA, double-stranded DNA, single-stranded RNA, or double-stranded RNA.
The isotope used for labeling the nucleic acid probe with the target isotope may be32P、3H or14Any one of C.
The medium used for electrophoresis in the method can be polyacrylamide gel.
The radioactive isotope has the same chemical properties and a small difference in molecular weight as compared with the corresponding non-radioactive isotope. Therefore, the isotopically labeled nucleic acid probe and the corresponding unlabeled nucleic acid fragment have the same mobility during gel electrophoresis. The DNA bands in the lanes of nucleic acid fragments can be visualized using the UV shading method. The DNA bands in the probe lanes cannot be visualized by UV shading because of insufficient content, but the positions of the full-length probes after electrophoresis are identical to those of the DNA bands in the nucleic acid fragment lanes. And cutting the gel block at the corresponding position of the probe lane, and eluting to obtain the full-length probe. This is the basic principle of the method.
Principle of ultraviolet shadow method: the gel is irradiated from above by an ultraviolet light source at the wavelength of 260nm, DNA in the gel absorbs ultraviolet light, and the DNA presents a dark blue strip under the fluorescent background of a paraffin film or a fluorescent thin-layer chromatography plate.
The method for gel cutting and purifying the isotope labeled nucleic acid probe uses the unlabeled nucleic acid fragment and the target isotope labeled nucleic acid probe to perform synchronous electrophoresis in different lanes of the same gel. The nucleic acid fragments were visualized using UV shading to indirectly determine the position of the full length probe on the gel. As only one piece of electrophoresis gel is needed to be used for one-time electrophoresis, the problems that two pieces of electrophoresis gel required by the method for gel cutting, purifying and isotopically labeling the nucleic acid probe in the background technology have the same chemical and physical properties and are required to be subjected to electrophoresis under the same conditions are fundamentally solved. Because two pieces of electrophoresis gel are avoided being used in sequence, the problem of nucleic acid strip deviation caused by the electrophoresis gel is fundamentally avoided, and the success rate of gel cutting and probe purification can be obviously improved. This is an advantageous effect of the present invention.
The invention consists of four steps, and the gel cutting purification method described in the background technology part needs six steps. The invention only needs one piece of electrophoresis gel for one time electrophoresis, while the gel cutting purification method described in the background technology part needs two pieces of electrophoresis gel for two times electrophoresis. Therefore, the invention is more concise and efficient, saves time and is simpler to operate. This is yet another advantageous effect of the present invention.
According to the gel cutting purification method, during electrophoresis, the nucleic acid fragments and the nucleic acid probes are positioned in two adjacent lanes on the electrophoresis gel. Therefore, after electrophoresis is finished, the nucleic acid fragments are displayed by using an ultraviolet shadow method, and the glue blocks at the corresponding positions of the probe lanes are easier to position.
In the gel cutting purification method of the present invention, two lanes adjacent to the nucleic acid probe lane are nucleic acid fragment lanes during electrophoresis. Therefore, after electrophoresis is finished, the nucleic acid fragments are displayed by using an ultraviolet shadow method, and the glue blocks at the corresponding positions of the probe lanes are easier to position.
The device for cutting gel and purifying isotope labeled nucleic acid probe consists of a base and a baffle, wherein an ultraviolet light source is arranged above the base, the ultraviolet light source is connected with the base through a bracket, and a color development panel is arranged on the base.
The color-developing panel is a fluorescent thin-layer chromatography plate. The color-developing panel may also be a parafilm.
With this structure, ultraviolet light with a wavelength of 260nm is emitted because the ultraviolet light source is located above. The gel is irradiated by ultraviolet light from above, and DNA in the gel absorbs the ultraviolet light, and the DNA presents a dark blue strip under the fluorescent background of a paraffin film or a fluorescent thin-layer chromatography plate. The full length probe is located in the probe lane at a position corresponding to this dark blue band. And cutting the gel block at the corresponding position of the probe lane, and eluting to obtain the full-length probe.
As an improvement of the gel cutting purification isotope labeling nucleic acid probe device, one or more holes are arranged on a base of the device and are matched with eppendorf centrifuge tubes with various specifications. The specifications of eppendorf centrifuge tubes include 0.5 ml, 1.5ml, 5ml, etc. After adopting such structure, it is very favorable to placing various eppendorf centrifuging tubes on this device. After the rubber is cut, the rubber block can be conveniently transferred to a centrifugal tube.
As a further improvement of the gel cutting purification isotope labeling nucleic acid probe device, a box body is arranged outside a baffle plate. After adopting such structure, can put into the box with the relevant consumptive material commonly used of experiment, like gloves, centrifuging tube etc to make things convenient for the completion of experiment flow. As optimization, a preservative film and a paraffin film are placed in the box body. Thus, the utility model can be conveniently used when needed.
The invention relates to a cutter for cutting electrophoretic glue, which consists of a cutter handle and a cutter head, wherein the cutter head is formed by surrounding four blades.
After the structure is adopted, the cutter head is formed by surrounding four blades, and the cut is in a shape of a Chinese character kou, so that the rubber block can be cut off only by cutting once. The common cutting knife, such as a scalpel, has a linear incision, and can cut off a rubber block only by cutting four times. Therefore, the cutter can obviously shorten the rubber cutting time. Meanwhile, the shape of the cut of the cutter is consistent with the shape of the nucleic acid strip after electrophoresis, so the cutter for cutting the gel is simpler and more convenient to use.
Isotopes have strong radioactivity which can induce cellular carcinogenesis and cause other damage to the human body. The damage to the human body from such radioactivity is proportional to the time it takes to operate the isotope. The longer the contact time with the isotope, the greater the damage. Therefore, the shortening of the operation time is very beneficial to the experimenters.
As an improvement of the electrophoretic gel cutting tool, four blades of the tool bit of the electrophoretic gel cutting tool are inclined inwards. After the structure is adopted, the four blades are inclined inwards, so that the circumference of the upper edge of the knife edge is larger than that of the lower edge of the knife edge, and the gel can be better pushed away by the upper edge of the knife edge when the gel is cut, so that the cut gel block is easier to separate from the original gel. Preferably, the blade is inclined inwardly at an angle of 5-15 degrees. Less than 5 degrees, the effect is not obvious. If the angle is larger than 15 degrees, the cut is too large in inclination, so that the cutting of the rubber block is not facilitated.
As a further improvement of the electrophoretic gel cutting tool, the tool handle and the tool bit of the electrophoretic gel cutting tool are fixedly connected, buckled and connected or screwed. And the cutter is more stable and durable due to the adoption of fixed connection. Adopt buckle connection or screw connection, then conveniently change the tool bit, be favorable to adapting to different rubber cutting scenes.
Drawings
The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments.
FIG. 1 is a flowchart of the method for gel cutting purification of an isotope-labeled nucleic acid probe according to the present invention.
FIG. 2 is a schematic diagram of a lane of the method for gel cutting purification of an isotope-labeled nucleic acid probe of the present invention.
FIG. 3 is a photograph of an autoradiogram of probes purified using the method of the present invention.
FIG. 4 is a schematic view showing the structure of the gel-cutting purified isotope labeled nucleic acid probe apparatus of the present invention.
Fig. 5 is a schematic structural diagram of the cutting tool for cutting electrophoretic gel according to the present invention.
Reference numerals: 1-base, 2-baffle, 3-bracket, 4-ultraviolet light source, 5-color panel, 6-hole digging, 7-box, 21-knife handle, 22-knife head, 23-blade and 26-notch.
Detailed Description
The following examples are intended to illustrate the invention and are not intended to limit the scope of the invention. The following examples are given without reference to specific experimental procedures or methods, generally according to conventional experimental conditions, such as those compiled by Sambrook et al, molecular cloning: the experimental conditions described in the laboratory Manual (New York: Cold Spring Harbor laboratory Press, 2002), or according to the manufacturer's recommendations.
The "isotope" referred to in the present invention generally means an isotope having radioactivity unless otherwise specified.
Example 1:
FIG. 1 is a flowchart of the method for gel cutting purification of an isotope-labeled nucleic acid probe according to the present invention. For comparison, the right side shows the conventional procedure for gel-cutting and purifying isotope-labeled nucleic acid probes. The dashed box indicates that the part requires handling of the radioisotope.
As shown in fig. 1, the method of the present invention comprises four steps. The method comprises the following steps: preparing a nucleic acid fragment corresponding to the target isotope-labeled nucleic acid probe; step two: separating the nucleic acid fragment and the target isotope labeled nucleic acid probe by electrophoresis; step three: positioning the position of the nucleic acid fragment on the electrophoresis gel by using an ultraviolet shadow method; step four: cutting the gel block at the position corresponding to the probe lane, and recovering the probe. The method of the invention is completed on one piece of electrophoresis gel, and only one time of electrophoresis is needed.
For comparison, the conventional procedure for gel-cutting and purifying an isotope-labeled nucleic acid probe consists of six steps. The method is divided into two stages, the first stage consists of the first three steps, and the second stage consists of the last three steps. The method needs two pieces of electrophoresis gel, and two pieces of electrophoresis gel are sequentially carried out. The first and second stages are sequentially run on different electrophoresis gels.
Preparing a target isotope labeled nucleic acid probe.
The method of the present invention is applicable to an isotope-labeled nucleic acid probe having a specific sequence, and can be prepared by various known methods. These methods include: nick translation method, end tailing method, end labeling method, reverse transcription doping method, in vitro transcription method and PCR method. The above methods for preparing isotopically labeled nucleic acid probes are available in a number of references and corresponding commercial products, such as molecular cloning: a laboratory Manual, a product related to Invitrogen corporation, a product related to Ambion corporation, and the like. The sequence of the nucleic acid probe may be single-stranded DNA, double-stranded DNA, single-stranded RNA or double-stranded RNA, and the labeled radioisotope may be32P、3H or14C。
It is an object of the present invention to provide a method for gel cutting and purification of an isotopically labeled nucleic acid probe, and therefore, the preparation of an isotopically labeled nucleic acid probe is only a working object of the present invention, and is not one of the steps of the present invention.
Preparing a nucleic acid fragment corresponding to the target isotope-labeled nucleic acid probe (step one).
The term "nucleic acid fragment corresponding to a target isotope-labeled nucleic acid probe" means that the nucleic acid fragment has the same base properties and base sequences as those of the target isotope-labeled nucleic acid probe. If the target isotope-labeled nucleic acid probe is a double-stranded DNA, the prepared nucleic acid fragment is also a double-stranded DNA and the base sequences of both are the same. If the target isotope-labeled nucleic acid probe is a single-stranded RNA, the prepared nucleic acid fragment is also a single-stranded RNA, and the base sequences of both are the same.
The nucleic acid fragment having the same sequence as that of the target isotope-labeled nucleic acid probe may be prepared by the same method as that for preparing the target isotope-labeled nucleic acid probe, or by other methods as long as the prepared nucleic acid fragment has the same base properties and base sequence as those of the target isotope-labeled nucleic acid probe. If the target isotope-labeled nucleic acid probe is prepared by the PCR method, the corresponding nucleic acid fragment may be prepared by the PCR method, or may be prepared by an artificial synthesis method.
And (2) separating the nucleic acid fragments and the target isotope labeled nucleic acid probe by electrophoresis (step two).
According to molecular cloning: the nucleic acid fragments and the target isotope-labeled nucleic acid probes are separated by electrophoresis using a gel electrophoresis method described in a laboratory manual or other literature references. The optimized electrophoretic medium is polyacrylamide gel.
Since the target isotope-labeled nucleic acid probe and the nucleic acid fragment corresponding thereto have the same properties and the difference in molecular weight is extremely small, the mobility is the same at the time of electrophoresis.
And (3) positioning the nucleic acid fragments on the electrophoresis gel by using an ultraviolet shadow method (step three).
FIG. 2 is a schematic diagram of a lane of the method for gel cutting purification of an isotope-labeled nucleic acid probe of the present invention. The nucleic acid fragments and the nucleic acid probes are positioned in two adjacent lanes on the electrophoresis gel, and the two lanes adjacent to the nucleic acid probe lane are nucleic acid fragment lanes, so that the lanes are distributed in a mode which is favorable for indicating the positions of the probes and facilitating the cutting of the gel block. Of course, other lane distributions may be used.
After electrophoresis is finished, a preservative film is placed on the gel, and the glass plate is reversed, so that the gel is attached to the preservative film. Then transferring the gel to the device for cutting gel and purifying the isotope labeled nucleic acid probe. And (3) turning on an ultraviolet light source, irradiating the gel from above at the wavelength of 260nm by the ultraviolet light source, absorbing the ultraviolet light by the DNA in the gel, and displaying a dark blue strip on the DNA under the fluorescent background of a paraffin film or a fluorescent thin-layer chromatography plate.
And cutting the gel block at the corresponding position of the probe lane, and recovering the probe (step four).
Because the radioactive isotope has the same chemical properties and small molecular weight difference compared with the corresponding non-radioactive isotope. Therefore, the isotopically labeled nucleic acid probe and the corresponding unlabeled nucleic acid fragment have the same mobility during gel electrophoresis.
As shown in lanes 1 and 3 of FIG. 2, the DNA bands in the lanes of nucleic acid fragments can be visualized by the UV shading method. As shown in lane 2 of FIG. 2, the DNA band in the probe lane could not be visualized by the UV shading method due to insufficient content, but the position of the full-length probe after electrophoresis was identical to the position of the DNA band in the nucleic acid fragment lane. The gel block at the corresponding position of the probe lane is cut, as shown in the box in lane 2 of FIG. 2. The electrophoresis gel cutting tool can be used for cutting, and a common scalpel can also be used for cutting.
The cut gel block was used "molecular cloning: the isotopically labeled nucleic acid probes in the gel mass are recovered by a method described in the laboratory Manual or commercially available products, such as Ambion, Progema, Inc.
Example 2:
and (3) synthesizing a nucleic acid probe.
Probes were synthesized using the RNA in vitro Transcription System RiboProbe in vitro Transcription System (Promega, USA) according to the kit instructions. Briefly described as follows: taking 4. mu.L of 5 Xbuffer, 2. mu.L of DTT (100 mM), 4. mu.L of ribonuclear Inhibitor 20u, rATP, rCTP and rGTP (2.5 mM each), 1. mu.l of pTRI-action-Mouse (0.5. mu.g/. mu.l, Ambion, USA) as transcription template, and 15u of SP6 RNA Polymerase [ alpha-32P]4 μ L of rUTP (800 Ci/mmol, 10 mCi/ml), 1 μ L of rUTP (100 μ M), made up to 20 μ L with water. And (5) uniformly mixing. The transcription was performed under the following conditions: 37 ℃ for 60 min.
And (3) synthesizing nucleic acid fragments.
RNA fragments were synthesized using the RNA in vitro Transcription System, Riboprobe in vitro Transcription System (Promega, USA) according to the kit instructions. Briefly described as follows: mu.L of 5 Xbuffer, 2. mu.L of DTT (100 mM), 4. mu.L of ribonuclear Inhibitor 20u, rATP, rCTP, rGTP and rUTP (2.5 mM each), 2. mu.L of pTRI-action-Mouse (0.5. mu.g/. mu.l, Ambion, USA) as a transcription template, and 15u of SP6 RNA Polymerase were added to 20. mu.L of water. And (5) uniformly mixing. The transcription was performed under the following conditions: 37 ℃ for 60 min.
Polyacrylamide gel electrophoresis.
The RNA fragments and transcripts of the RNA probe were separated by electrophoresis at a constant pressure of 250V for 60min using 5% polyacrylamide/8M urea gel (0.75 mm thick).
The nucleic acid probe with the length of 334 nt is purified by cutting gel.
And (3) preparing an RNA probe by taking pTRI-Actin-Mouse as a template and performing catalytic reaction by using SP6 RNA polymerase. And an RNA fragment of 334 nt was obtained in the same manner. The separation was performed by electrophoresis at a constant pressure of 250V for 60min using 5% polyacrylamide/8M urea gel (0.75 mm thick). And (3) revealing the position of the nucleic acid fragment by using an ultraviolet shadow method, cutting a glue block at the corresponding position in a probe lane for elution, and recovering the probe. After gel electrophoresis again, exposure was performed using autoradiography. FIG. 3 is an autoradiogram of the probe purified by the method of the present invention, confirming successful probe recovery.
Example 3:
FIG. 4 is a schematic view showing the structure of the gel-cutting purified isotope labeled nucleic acid probe apparatus of the present invention. The gel cutting purification isotope labeling nucleic acid probe device shown in fig. 4 is composed of a base 1 and a baffle 2, an ultraviolet light source 4 is arranged above the base 1, the ultraviolet light source 4 is connected with the base 1 through a support 3, and a color development panel 5 is arranged on the base 1. The color development panel 5 is a fluorescent thin-layer chromatography plate, and may be a paraffin film. Two digging holes 6 are arranged on the base 1, and the two digging holes 6 are respectively matched with 1.5ml eppendorf centrifuge tubes and 5ml eppendorf centrifuge tubes. A box body 7 is arranged on the outer side of the baffle plate 2. Preservative films and paraffin films are placed in the box body 7, and other objects required by experiments can be placed in the box body 7.
The using method comprises the following steps: after the gel electrophoresis is finished, a preservative film is placed on the gel, and the glass plate is inverted, so that the gel is attached to the preservative film. The gel was then transferred to the apparatus. And (3) turning on an ultraviolet light source, irradiating the gel from above at the wavelength of 260nm by the ultraviolet light source, absorbing the ultraviolet light by the DNA in the gel, and displaying a dark blue strip on the DNA under the fluorescent background of a paraffin film or a fluorescent thin-layer chromatography plate. The glue cutting operation can be performed.
Example 4:
fig. 5 is a schematic structural diagram of the cutting tool for cutting electrophoretic gel according to the present invention. The cutter for cutting electrophoretic gel shown in fig. 5 comprises a handle 21 and a cutter head 22, wherein the cutter head 22 is surrounded by four blades 23. The angle of inward inclination of the blades 23 of the cutter head 22 is 0 degree, but may be other angles such as 5 degrees, 10 degrees, 15 degrees, and the like. The tool holder 21 and the tool bit 22 are fixedly connected, and other connection methods such as a snap connection method and a screw connection method can be adopted.
The using method comprises the following steps: the cutter for cutting the electrophoretic gel has the same use mode as other common cutters, such as scalpels. Because the tool bit of the tool of the invention consists of four blades, one square cut 26 can be finished by one-time cutting, so that the rubber blocks are separated, and the time is saved greatly.
After reading the above description of the present invention, the skilled person can make various modifications or alterations to the present invention, such as using agarose gel instead of polyacrylamide gel, using other isotopes, other conventional staining methods instead of the corresponding parts of the present invention, which equivalents are also within the scope of the invention as defined in the appended claims.
Claims (10)
1. The utility model provides a device of gel cutting purification isotope labeling nucleic acid probe, comprises base (1) and baffle (2), its characterized in that: an ultraviolet light source (4) is arranged above the base (1), the ultraviolet light source (4) is connected with the base (1) through a support (3), and a color development panel (5) is arranged on the base (1).
2. The apparatus for gel cutting purification of an isotope-labeled nucleic acid probe according to claim 1, wherein: the color development panel (5) is a fluorescent thin-layer chromatography plate.
3. The apparatus for gel cutting purification of an isotope-labeled nucleic acid probe according to claim 1, wherein: the color development panel (5) is a paraffin film.
4. The apparatus for gel cutting purification of an isotope-labeled nucleic acid probe according to any one of claims 1 to 3, characterized in that: the base (1) is provided with one or more digging holes (6), and the digging holes (6) are matched with eppendorf centrifuge tubes of various specifications.
5. The apparatus for gel cutting purification of an isotope-labeled nucleic acid probe according to any one of claims 1 to 3, characterized in that: a box body (7) is arranged on the outer side of the baffle (2).
6. The apparatus for gel cutting purification of an isotope-labeled nucleic acid probe according to claim 5, wherein: the preservative film and the paraffin film are placed in the box body (7).
7. The utility model provides a cutter of cutting electrophoresis gel, comprises handle of a knife (21) and tool bit (22), its characterized in that: the cutter head (22) is formed by encircling four blades (23).
8. The cutter for cutting electrophoretic glue according to claim 7, wherein: the four blades (23) of the cutter head (22) are inwardly inclined.
9. The cutter for cutting electrophoretic glue according to claim 8, wherein: the inward inclined angle of the blade (23) of the cutter head (22) is 5-15 degrees.
10. A cutter for cutting an electrophoretic gel, as claimed in any one of claims 7 to 9, wherein: the knife handle (21) and the knife head (22) are fixedly connected, buckled or screwed.
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| CN112280777A (en) * | 2020-11-06 | 2021-01-29 | 贵州师范学院 | Method for cutting gel and purifying isotope labeled nucleic acid probe |
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