CN112244825A - Method for low-loss extraction of shellfish blood - Google Patents
Method for low-loss extraction of shellfish blood Download PDFInfo
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- CN112244825A CN112244825A CN202011239529.0A CN202011239529A CN112244825A CN 112244825 A CN112244825 A CN 112244825A CN 202011239529 A CN202011239529 A CN 202011239529A CN 112244825 A CN112244825 A CN 112244825A
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- blood
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- shellfish
- individuals
- clam
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- 210000004369 blood Anatomy 0.000 title claims abstract description 50
- 239000008280 blood Substances 0.000 title claims abstract description 50
- 238000000034 method Methods 0.000 title claims abstract description 38
- 235000015170 shellfish Nutrition 0.000 title claims abstract description 25
- 238000000605 extraction Methods 0.000 title claims abstract description 7
- 210000003205 muscle Anatomy 0.000 claims abstract description 10
- 239000013535 sea water Substances 0.000 claims abstract description 10
- 239000003242 anti bacterial agent Substances 0.000 claims abstract description 4
- 229940088710 antibiotic agent Drugs 0.000 claims abstract description 4
- 238000011084 recovery Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000005303 weighing Methods 0.000 claims abstract description 4
- 238000005070 sampling Methods 0.000 abstract description 4
- 241001465754 Metazoa Species 0.000 abstract description 3
- 238000010171 animal model Methods 0.000 abstract description 3
- 238000009395 breeding Methods 0.000 abstract description 3
- 230000001488 breeding effect Effects 0.000 abstract description 3
- 231100000518 lethal Toxicity 0.000 abstract description 3
- 230000001665 lethal effect Effects 0.000 abstract description 3
- 238000011160 research Methods 0.000 abstract description 3
- 238000009394 selective breeding Methods 0.000 abstract description 3
- 241000894007 species Species 0.000 abstract description 3
- 238000011161 development Methods 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150015—Source of blood
- A61B5/15003—Source of blood for venous or arterial blood
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/15—Devices for taking samples of blood
- A61B5/150007—Details
- A61B5/150206—Construction or design features not otherwise provided for; manufacturing or production; packages; sterilisation of piercing element, piercing device or sampling device
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/40—Animals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/42—Evaluating a particular growth phase or type of persons or animals for laboratory research
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Heart & Thoracic Surgery (AREA)
- Medical Informatics (AREA)
- Molecular Biology (AREA)
- Surgery (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Veterinary Medicine (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Manufacturing & Machinery (AREA)
- Farming Of Fish And Shellfish (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a method for extracting shellfish blood with low loss, which selects healthy individuals with intact appearance; weighing the selected individuals, and calculating the safe blood drawing amount; a small hole is arranged above the adductor muscle by a gem cutting machine; inserting a disposable sterile syringe into the place of the thick adductor muscle of the clam for 0.5-1cm to draw blood, and controlling the blood drawing amount; putting the individuals with the blood drawn back into clean seawater for culture, and adding antibiotics into the water; and determining the next blood drawing time according to the recovery condition of the clam and the experimental requirement. The method solves the problem of experimental data loss caused by parent death in the traditional breeding process, can extract the required data from the parent by blood extraction when the parent is still alive, and does not influence the subsequent growth of the parent. Compared with the traditional lethal sampling method, the method has the advantages that shellfish blood is extracted with low damage, great damage to experimental animals is avoided, and the method has important research significance and practical value in the aspects of endangered animal resource protection, economic species whole-gene selective breeding and the like.
Description
Technical Field
The invention relates to the field of shellfish blood extraction, in particular to a method for extracting shellfish blood with low loss.
Background
The current methods for extracting shellfish blood in the market are mostly methods for destroying shellfish tissue structures, so that the purpose of obtaining shellfish blood is achieved. Most of these methods destroy the mantle of the shellfish, resulting in death of the shellfish in a short time. The prior art can not complete the academic sampling of the parent individuals in different growth periods, which leads to the reduction of the experimental efficiency.
Disclosure of Invention
The invention aims to provide a method for extracting shellfish blood with low loss aiming at the defects of the prior art so as to solve the problems of the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a method for extracting shellfish blood with low loss comprises the following steps:
the method comprises the following steps: selecting healthy individuals with intact appearances;
step two: weighing the selected individuals, and calculating the safe blood drawing amount;
step three: a small hole is arranged above the adductor muscle by a gem cutting machine;
step four: inserting a disposable sterile syringe into the place of the thick adductor muscle of the clam for 0.5-1cm to draw blood, and controlling the blood drawing amount;
step five: putting the individuals with the blood drawn back into clean seawater for culture, and adding antibiotics into the water;
step six: and determining the next blood drawing time according to the recovery condition of the clam and the experimental requirement.
As a preferable technical scheme of the invention, the diameter of the small hole in the step three is 0.3-0.6 mm.
As a preferable technical scheme of the present invention, the seawater in the fifth step is sterilized seawater.
The invention has the beneficial effects that: the method solves the problem of experimental data loss caused by parent death in the traditional breeding process, can extract the required data from the parent by blood extraction when the parent is still alive, and does not influence the subsequent growth of the parent. Compared with the traditional lethal sampling method, the method has the advantages that shellfish blood is extracted with low damage, great damage to experimental animals is avoided, and the method has important research significance and practical value in the aspects of endangered animal resource protection, economic species whole-gene selective breeding and the like.
The method can repeatedly take blood for many times (safe blood taking amount does not affect the growth and development of the shellfish); adopting shellfish blood to extract genetic material, and determining enzyme activity in blood serum; the physiological indexes of different periods can be measured on the same shellfish, so that individual difference is avoided, and the experiment precision is improved.
Drawings
FIG. 1 is a flow chart of the method of the present invention;
FIG. 2 is a flow chart of the present invention for extracting blood from clam;
FIG. 3 is a line graph of the amount of blood drawn from the clam of the present invention and the weight of the clam.
Detailed Description
The following detailed description of the preferred embodiments of the present invention, taken in conjunction with the accompanying drawings, will make the advantages and features of the invention more readily understood by those skilled in the art, and thus will more clearly and distinctly define the scope of the invention.
Referring to fig. 1, the present invention provides a technical solution: a method for extracting shellfish blood with low loss comprises the following steps:
the method comprises the following steps: selecting healthy individuals with intact appearances;
step two: weighing the selected individuals, and calculating the safe blood drawing amount;
step three: a small hole is arranged above the adductor muscle by a gem cutting machine;
step four: inserting a disposable sterile syringe into the place of the thick adductor muscle of the clam for 0.5-1cm to draw blood, and controlling the blood drawing amount;
step five: putting the individuals with the blood drawn back into clean seawater for culture, and adding antibiotics into the water;
step six: and determining the next blood drawing time according to the recovery condition of the clam and the experimental requirement.
The diameter of the small hole in the third step is 0.3-0.6 mm; and the seawater in the fifth step is sterilized seawater.
Example 1: from fig. 2-3, we can find the relationship between the maximum amount of blood drawn from the thick adductor muscle of clam and the body weight based on this method, and determine the amount of blood drawn according to the size of clam. The shellfish growth and development after the blood drawing amount exceeds 30 percent of the maximum blood drawing amount (at the thick adductor muscle), and the shellfish death can be caused when the blood drawing amount exceeds 50 percent.
The method solves the problem of experimental data loss caused by parent death in the traditional breeding process, can extract the required data from the parent by blood extraction when the parent is still alive, and does not influence the subsequent growth of the parent. Compared with the traditional lethal sampling method, the method has the advantages that shellfish blood is extracted with low damage, great damage to experimental animals is avoided, and the method has important research significance and practical value in the aspects of endangered animal resource protection, economic species whole-gene selective breeding and the like.
The method can repeatedly take blood for many times (safe blood taking amount does not affect the growth and development of the shellfish); adopting shellfish blood to extract genetic material, and determining enzyme activity in blood serum; the physiological indexes of different periods can be measured on the same shellfish, so that individual difference is avoided, and the experiment precision is improved.
The above examples only show some embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention.
Claims (3)
1. A method for low-loss extraction of shellfish blood, characterized by: the method comprises the following specific steps:
the method comprises the following steps: selecting healthy individuals with intact appearances;
step two: weighing the selected individuals, and calculating the safe blood drawing amount;
step three: a 0.3-0.6mm small hole is arranged above the adductor muscle by a gem cutting machine;
step four: inserting a disposable sterile syringe into the place of the thick adductor muscle of the clam for 0.5-1cm to draw blood, and controlling the blood drawing amount;
step five: putting the individuals with the blood drawn back into clean seawater for culture, and adding antibiotics into the water;
step six: and determining the next blood drawing time according to the recovery condition of the clam and the experimental requirement.
2. The method of claim 1, wherein the method comprises the steps of: the diameter of the small hole in the third step is 0.3-0.6 mm.
3. The method of claim 1, wherein the method comprises the steps of: and the seawater in the fifth step is sterilized seawater.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011239529.0A CN112244825A (en) | 2020-11-09 | 2020-11-09 | Method for low-loss extraction of shellfish blood |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011239529.0A CN112244825A (en) | 2020-11-09 | 2020-11-09 | Method for low-loss extraction of shellfish blood |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112244825A true CN112244825A (en) | 2021-01-22 |
Family
ID=74266502
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011239529.0A Pending CN112244825A (en) | 2020-11-09 | 2020-11-09 | Method for low-loss extraction of shellfish blood |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112244825A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4954340A (en) * | 1988-04-23 | 1990-09-04 | Juridical Foundation The Chemo-Sero-Therapeutic Research Institute | Method for activating hemocytes of bivalves for pearl production |
| US7013836B1 (en) * | 2002-06-27 | 2006-03-21 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Enhancing maturation of oocytes in bivalves |
| US20100162964A1 (en) * | 2008-12-29 | 2010-07-01 | Dalian Fisheries University | Process for polycross breeding Manila clam pedigree |
| CN107817259A (en) * | 2017-10-30 | 2018-03-20 | 中国水产科学研究院黄海水产研究所 | A kind of method of seawater blood clam section shellfish blood cell collection and Ultrastructural observation |
| CN108931468A (en) * | 2018-05-16 | 2018-12-04 | 中国海洋大学 | A kind of method of easy quickly detection oyster ploidy |
-
2020
- 2020-11-09 CN CN202011239529.0A patent/CN112244825A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4954340A (en) * | 1988-04-23 | 1990-09-04 | Juridical Foundation The Chemo-Sero-Therapeutic Research Institute | Method for activating hemocytes of bivalves for pearl production |
| US7013836B1 (en) * | 2002-06-27 | 2006-03-21 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Enhancing maturation of oocytes in bivalves |
| US20100162964A1 (en) * | 2008-12-29 | 2010-07-01 | Dalian Fisheries University | Process for polycross breeding Manila clam pedigree |
| CN107817259A (en) * | 2017-10-30 | 2018-03-20 | 中国水产科学研究院黄海水产研究所 | A kind of method of seawater blood clam section shellfish blood cell collection and Ultrastructural observation |
| CN108931468A (en) * | 2018-05-16 | 2018-12-04 | 中国海洋大学 | A kind of method of easy quickly detection oyster ploidy |
Non-Patent Citations (1)
| Title |
|---|
| 余友茂: "珍稀海产动物——鲎", 《福建水产》 * |
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