CN112243955A

CN112243955A - Construction method and application of novel PLS3 gene knockout rat animal model

Info

Publication number: CN112243955A
Application number: CN202011150353.1A
Authority: CN
Inventors: 李梅; 夏维波; 胡静; 孙磊
Original assignee: Peking Union Medical College Hospital Chinese Academy of Medical Sciences
Current assignee: Peking Union Medical College Hospital Chinese Academy of Medical Sciences
Priority date: 2020-10-23
Filing date: 2020-10-23
Publication date: 2021-01-22
Anticipated expiration: 2040-10-23
Also published as: CN112243955B

Abstract

The invention discloses a construction method and application of a novel PLS3 gene knockout rat animal model. The invention firstly carries out targeted knockout on an E10-E16 fragment of PLS3 gene in a cell to obtain PLS3 gene mutant cell, and then constructs a novel PLS3 gene knockout animal model by using the obtained cell. The novel animal model constructed by the invention makes up the defects of animal models related to osteoporosis heredity, provides a reliable and effective novel experimental animal model for researching the genetic mechanism of osteoporosis, and has important application value.

Description

Construction method and application of novel PLS3 gene knockout rat animal model

Technical Field

The invention belongs to the technical field of biomedicine, and particularly relates to a construction method and application of a novel PLS3 gene knockout rat animal model.

Background

Osteoporosis is a systemic bone disease associated with increased age characterized by decreased bone strength and increased risk of fracture. Epidemiological reports published by the national health committee in 2018 show that the prevalence rate of osteoporosis of people over 50 years old in China is as high as 19.2%, the prevalence rate of osteoporosis is high, and the prevalence rate still increases rapidly (Chinese medical society, the subcompartment of osteoporosis and bone and mineral diseases, Chinese osteoporosis epidemiological investigation and action results of the 'healthy bones' special item are published [ J ]. J. J. China osteoporosis and bone and mineral diseases, 2019,12(4):317 and 318). The most serious consequence of osteoporosis is brittle fracture, which can cause restricted activity, skeletal malformation, acute complications of cardiovascular and cerebrovascular diseases, seriously affect the quality of life of patients and also bring heavy burden to the social economy (China medical society, bone and mineral diseases, J.J.Chinese osteoporosis and bone and mineral diseases, 2017,10(5): 413-443). Deeply exploring the pathogenesis of the disease, establishing a new strategy for preventing and treating the disease, and being the key for practically reducing the burden of the disease and restraining the harm of the disease.

Osteoporosis is a complex disease regulated by multiple factors such as heredity, environment, endocrine and the like. In recent years, it has been found that genetic factors play an important role in the development of disease, and 80% of peak bone mass is regulated by genetic factors. More than 500 genetic susceptibility sites have been found to be closely related to bone mass, fracture risk, but these can account for only about 20% of skeletal phenotypes (Morris J A, Kemp J P, Youlten S E, et al. an atlas of genetic inflections on osteoporosis in humanans and mice [ J ] Nat Genet,2019,51(2): 258-). At present, the genetic research of osteoporosis faces challenges, results of different populations, different ethnicities and different research methods have inconsistency, and models for researching the genetic risk of osteoporosis are extremely deficient. In recent years, studies have found that osteoporosis and bone fractures are often characterized by an increased maternal genetic risk, in which the bone regulatory gene PLS3 may play an important role (Kim KM, Kim YJ, Choi SH, et al, the effects of body mass indexes on the genetic factors in bone mass in heat-resistant nails (KNHANES V) [ J ] Osteoporos Int,2016,27(6): 2057). The PLS3 (plastic 3) gene is located in Xq23 chromosome (NG-012518) of human, encodes PLS3 protein, is an actin-binding protein (actin-binding protein) family member, is widely expressed in human solid tissues, is involved in regulating actin polymerization, fasciation or crosslinking, and influences cell structure and function (Delanote V, Vankeckhove J, Gettermans J. plastics: upside molecular modules of actin organization in (path) physiological cellular processes [ J ] Acta Pharmacol Sin,2005,26(7): 769-779). Since 2013, various studies report that PLS3 gene mutation can cause X chromosome associated with hereditary osteogenesis imperfecta or osteoporosis, and patients have the characteristics of reduced bone density and increased bone fragility, and are characterized by repeated limb fracture and spinal compression fracture under slight external force (an Dijk FS, Zillikens MC, Michad, et al.PLS3 tissues in X-linked osteoporotosis with fractures [ J ]. N Engl J Med,2013,369 (16: 1529) -1536). Population cohort studies have shown that the PLS3 single nucleotide polymorphism is significantly associated with female fracture risk and low bone mass (Shao C, Wang YW, He JW, et al. The above studies all suggest that PLS3 is a key candidate gene in the genetic regulatory mechanism of osteoporosis. Although a PLS3 mutant mouse model has been developed, it does not sufficiently interpret the phenotype of the disease (Neugebauer J, Heilig J, Hosseini barkooi S, et al, Plastic 3 inflammation bone hormone disorders through regulation of osteoinductive activity [ J ]. Hum Mol Gene, 2018,27(24): 4249-.

The applicant team discovers maternal hereditary osteoporosis caused by deletion mutation of large fragments of 10 th-16 th exons (E10-E16) of PLS3 gene for the first time in clinical diagnosis, patients have typical osteoporosis phenotypes such as obvious reduction of bone mass and multi-vertebral compression fracture, and gene sequencing discovers that micro-homologous mediated end ligation participates in mediating the occurrence of large fragment deletion of PLS3 (Lv F, Ma M, Liu W, et al. A novel large fragment deletion in PLS3 mice X-linked early-associated osteoporosis and stress to zoledronic acid [ J ]. Osteporosis Int,2017,28(9): 2691-2700). From the gene mutation of the patient, an experimental animal with high identity with the human PLS3 gene is selected, small guide RNA (small guide RNA, sgRNA) is synthesized by means of a high-efficiency and accurate CRISPR/Cas9 gene editing system aiming at the E10-E16 fragment of the PLS3 gene, the sgRNA is combined with a specific target under the mediation of a pro-spacer adjacent motif (PAM), and the target sequence is subjected to specific knockout by using a Cas9 enzyme, so that a model animal (Doudna JA, Charpentier E.genome editing.the new front of genome engineering with CRISPR-Cas9[ J ]. Science,2014,346(6213):1258096) with the genotype in accordance with clinical practice is constructed. The PLS3 protein is highly conserved among different species, and the homology of the human PLS3 protein and the PLS3 protein of mammals such as rats, mice, dogs, cows and the like is as high as more than 97%, which suggests that the mammals such as the rats, the mice and the like can be used as an ideal object for the PLS3 gene knockout. The genotype of the animal model is derived from clinical osteoporosis patients, and the animal model has novelty and important clinical application value, provides important animal experimental research basis for deeply researching genetic mechanism of osteoporosis occurrence and establishing new diagnosis and treatment strategies by taking PLS3 related bone matrix protein metabolic pathways as targets, has important value in the aspect of researching genetic regulation and control of bone metabolism, and has a key role in the pathogenesis of tumors and motor neuron diseases by PLS3, and the animal model provided by the invention also has important potential application value in the disease research.

Disclosure of Invention

In order to deeply research the genetic regulation mechanism of osteoporosis and make up the defects of related animal models in the field, the invention aims at important skeletal metabolism regulation gene PLS3 positioned on an X chromosome, and starts from the large-fragment deletion genotype of PLS3 causing hereditary osteoporosis, realizes the directional mutation of PLS3 gene and constructs a brand-new osteoporosis animal model with PLS3 gene specific fragment deletion.

In order to achieve the purpose, the invention adopts the following technical scheme:

the invention provides a method for constructing a novel PLS3 gene knockout rat animal model.

Further, the construction method comprises the following steps:

(1) and (3) carrying out targeted knockout on a specific fragment of the PLS3 gene in the cell to prepare a PLS3 gene mutant cell.

(2) And (3) constructing a novel PLS3 gene knockout animal model by using the PLS3 gene mutation cell obtained in the step (1).

Further, the specific fragment is an E10-E16 fragment of PLS3 gene.

Further, the construction method includes a knock-out method of a specific fragment of PLS3 gene in a cell.

Further, the knockout method adopts a CRISPR/Cas9 gene editing system, and realizes PLS3 gene targeted mutation by introducing sgRNA and Cas9 into a receptor cell.

Further, the recipient cell is preferably a fertilized egg.

Further, the specific fragment is derived from a clinically actual patient gene mutant, and the CRISPR/Cas9 system comprises a sgRNA expression vector targeting PLS3 gene E10-E16.

Further, the sgRNA expression vector includes sgRNA1, sgRNA2, sgRNA3, and/or sgRNA 4.

Further, the target sequence of the sgRNA1 is (1), (2) or (3) below:

(1) a sequence shown as SEQ ID NO.1 in a sequence table;

(2) a DNA fragment derived from (1) and having 75% or more 75% identity with the DNA sequence defined in (1);

(3) a DNA fragment derived from (1) which hybridizes with the DNA sequence defined in (1) under stringent conditions.

Further, the target sequence of the sgRNA2 is (1), (2) or (3) below:

(1) a sequence shown as SEQ ID NO.2 in the sequence table;

Further, the target sequence of the sgRNA3 is (1), (2) or (3) below:

(1) a sequence shown as SEQ ID NO. 3in the sequence table;

Further, the target sequence of the sgRNA4 is (1), (2) or (3) below:

(1) a sequence shown as SEQ ID NO.4 in the sequence table;

Preferably, the sequence of the sgRNA1 is shown as SEQ ID No. 5-6, the target sequence is shown as SEQ ID No.1, the sequence of the sgRNA2 is shown as SEQ ID No. 7-8, the target sequence is shown as SEQ ID No.2, the sequence of the sgRNA3 is shown as SEQ ID No. 9-10, the target sequence is shown as SEQ ID No.3, the sequence of the sgRNA4 is shown as SEQ ID No. 11-12, and the target sequence is shown as SEQ ID No. 4.

In an embodiment of the invention, the method comprises introducing the sgRNA and Cas9 into a recipient cell to realize the targeted mutation of the PLS3 gene, and introducing the sgRNA and Cas9 into the recipient cell comprises injecting the sgRNA and Cas9 into the recipient cell by means of microinjection, wherein the recipient cell contains the PLS3 gene.

The term "identity" as used herein refers to sequence similarity to a native nucleic acid sequence. "identity" includes nucleotide sequences that are 75% or greater, or 85% or greater, or 90% or greater, or 95% or greater identical to the nucleotide sequences of the present invention. The nucleotide sequence can be evaluated visually or by computer software. Using computer software, the identity between two or more sequences can be expressed in percent (%), which can be used to assess the identity between related sequences.

The identity of 75% or more may be 80%, 85%, 90% or 95% or more.

The term "stringent conditions" refers to any of the usual, standard experimental conditions carried out according to the Southern Blotting principle, and the following are exemplary and not limiting: stringent conditions may be hybridization with Hyb high performance hybridization overnight at 42 deg.C, rinsing 2 times 5min each with a solution of 2 XSSC, 0.1% SDS at room temperature, and rinsing 2 times 15min each with a solution of 0.1 XSSC, 0.1% SDS at 65 deg.C.

The sgRNA is transcribed into RNA by respective target sequences and is connected with a sgRNA framework sequence to form a sgRNA expression vector. The framework sequence of the sgRNA can be specifically transcribed from a DNA sequence corresponding to the framework sequence of the sgRNA in pUC57 (addge).

The Cas9 expression vector includes a Cas9 universal expression vector.

Such mutations include those which cause an alteration in the function of PLS3 gene.

The PLS3 gene mutant cell prepared by the method of the target mutation of the PLS3 gene in the cell also belongs to the protection scope of the invention.

Further, the cell is a non-germ cell.

Further, the animal in the step (2) of the construction method is a mammal, preferably a rat.

Further, the sgrnas include sgrnas and sgRNA-associated biomaterials.

Further, the sgRNA-associated biological material is selected from any one of the following (1) to (7):

(1) a nucleic acid molecule encoding the sgRNA;

(2) an expression cassette comprising the nucleic acid molecule of (1);

(3) a recombinant vector comprising the nucleic acid molecule of (1), or a recombinant vector comprising the expression cassette of (2);

(4) a recombinant microorganism containing (1) the nucleic acid molecule, or a recombinant microorganism containing (2) the expression cassette, or a recombinant microorganism containing (3) the recombinant vector;

(5) a transgenic animal cell line containing the nucleic acid molecule of (1) or a transgenic animal cell line containing the expression cassette of (2);

(6) a transgenic animal tissue containing the nucleic acid molecule of (1) or a transgenic animal tissue containing the expression cassette of (2);

(7) a transgenic animal organ containing the nucleic acid molecule of (1) or a transgenic animal organ containing the expression cassette of (2).

Further, the nucleic acid molecule includes DNA, such as cDNA, genomic DNA, or recombinant DNA; the nucleic acid molecule also includes RNA, such as mRNA or hnRNA, and the like.

Further, the expression cassette refers to a DNA capable of expressing the sgRNA in a host cell, and the DNA may include not only a promoter that initiates transcription of a gene encoding the sgRNA but also a terminator that terminates transcription of the encoding gene.

Further, the expression cassette may also include an enhancer sequence.

The transgenic animal cell line, the transgenic animal tissue and the transgenic animal organ do not comprise propagation materials.

The second aspect of the present invention provides the use of the production method according to the first aspect of the present invention in any one of the following (1) to (3):

(1) use in the study of osteoporosis and/or other bone diseases;

(2) the application in the research of tumor diseases;

(3) use in the study of spinal muscular atrophy and/or other motor neuron diseases.

A third aspect of the present invention provides use of the sgRNA or sgRNA-related biomaterial according to the first aspect of the present invention in any one of the following (1) to (7):

(1) the application of the mutant in constructing PLS3 gene cells;

(2) the use thereof for the preparation of a cell product for the construction of a PLS3 gene mutation;

(3) the application in preparing PLS3 gene mutation animals;

(4) the use thereof for the preparation of an animal product for the construction of a PLS3 gene mutation;

(5) use in the study of osteoporosis and/or other bone diseases;

(6) the application in the research of tumor diseases;

(7) use in the study of spinal muscular atrophy and/or other motor neuron diseases.

The invention has the advantages and beneficial effects that:

the invention provides a method for the targeted deletion mutation of a specific segment of a PLS3 gene (E10-E16 segment of a PLS3 gene) and an animal model constructed by the method for the first time, wherein the gene mutation site and the type are from osteoporosis patients of clinical actual X chromosome sex-linked inheritance, and the model makes up the defects of an osteoporosis genetic related animal model, provides a reliable and effective experimental animal model for researching the genetic mechanism of osteoporosis, provides a good animal basis for establishing a novel strategy for preventing, diagnosing and treating osteoporosis, and has important application value. In addition, the invention also has potential application value in the aspects of tumor, motor neuron diseases and the like.

Drawings

FIG. 1 is a technical scheme for constructing a PLS3 gene mutation rat model;

FIG. 2 is a schematic diagram of 4 targeted knock-out sites designed for exons 10-16 of the PLS3 gene;

FIG. 3 is a drawing of an electrophoresis band for genotyping of rat generation F0;

FIG. 4 is a genotype identification electrophoretic band of F1-generation rats obtained after crossing F0-generation mutant female rats and wild-type SD rats.

Detailed Description

The present invention will be described in further detail with reference to the following detailed description and the accompanying drawings, which are provided for illustration only and are not intended to limit the scope of the present invention. It is to be noted that modifications or substitutions may be made in the spirit and form of the technical solution of the present invention without departing from the subject and scope of the present invention, but those modifications and substitutions are within the scope of the present invention.

Example construction of PLS3 Gene Targeted mutant rat model and identification of genotype

The invention utilizes CRISPR/Cas9 technology to carry out large-fragment targeted knockout on 10 th-16 th exons (E10-E16) of PLS3 Gene [ Rattus norvegicus (Norway rat) ], wherein the reference sequence is PLS3 Gene sequence (SEQ ID NO.17) in a sequence table, information is derived from NCBI database (NCBI Gene ID:81748, https:// www.ncbi.nlm.nih.gov/Gene/81748), and the technical route of specific model construction is shown in figure 1.

1. Experimental methods

(1) Design of target Gene knockout targets

According to the PLS3 gene sequence, 4 acting targets (see table 1) as shown in figure 2 are designed aiming at the intention knock-out large fragment, the target sequence is determined by using an online Design Tool (CRISPR Design Tool, http:// tools, genome-engineering. org), and four pairs of oligonucleotide chains (DNA oligonucleotides, see table 2) for recognizing the target site are synthesized.

Table 1 shows the 4 action targets and PAM sequences of PLS3 gene E10-16

Table 2 oligonucleotide strands identifying exons 10-16 of rat PLS3

(2) Preparation of sgRNA and Cas9-RNA

The DNA Oligos were annealed to double strands and ligated to pUC57-sgRNA expression vector (Addgene ID:51132) linearized with Bsa I endonuclease to construct a sgRNA expression vector. pST1374-NLS-flag-linker-Cas9(Addgene ID:44758) was selected as the Cas9 expression vector. sgRNA and Cas9 expression vectors were transcribed into injectable sgRNA and Cas9-RNA using an in vitro transcription kit (Ambion Am 1345).

(3) Preparation of F0-generation rats

Selecting 15 SD female mice of 4-6 weeks old, injecting 20 IU/mouse of Pregnant Mare Serum Gonadotropin (PMSG) into abdominal cavity, and injecting 20 IU/mouse of human chorionic gonadotropin (hCG) after 48h, and performing superovulation. According to the following steps: 1 ratio and adult male mice, collecting about 150 fertilized eggs for microinjection. 30 vasectomized male mice of 8-10 weeks are prepared at the same time, and the male mice are combined with adult female mice 1d before ovulation to prepare pseudopregnant receptor female mice.

Cas9-RNA obtained by in vitro transcription and sgRNA are mixed, after the concentration is adjusted, the RNA mixture is injected into the male pronucleus and cytoplasm of fertilized eggs of SD rats by a microinjection method (TE2000U microinjector), and then the fertilized eggs are implanted into bodies of pseudopregnant SD rats.

(4) Genotype identification of F0 generation PLS3 mutant rats

Taking F0 newborn suckling mouse toes from 7-14d, extracting with genome DNA extraction kit (

Genomic DNA Kit EE101-12, Beijing Pan-Kagaku Co.) was extracted from rat Genomic DNA. Based on the detection of WT primers for the wild-type gene and KO primers for the mutant-type gene (Table 3) designed based on the sequence information, the target gene was amplified by PCR, the amplified products were separated by electrophoresis on 1.0% agarose gel, and the specific information of the knock-out fragment was determined by gene sequencing.

TABLE 3 primer names for PLS3 Gene amplification and sequences thereof

TABLE 4 size of amplified product of WT primer and KO primer for F0 mutant mice

(5) F1 generation PLS3 mutant rat breeding and genotype identification

The 6F 0 generation mutant rats are cultured according to 6 strains respectively, and the rats without Specific Pathogen (SPF) are raised in the barrier environment of experimental animals under the condition of 12h light/12 h dark light cycle, the temperature is 20-25 ℃, the relative humidity is 50-65%, and the supply of sufficient feed and water is ensured. And breeding the female F0 generation mutant rats and the male F0 generation mutant rats to wild type SD rats in a ratio of 1:1 and a ratio of 1:1 or 1:2 when the female rats and the male F0 generation mutant rats are bred to 2-3 months of age, wherein female rats of male F0 mutant rat offspring are heterozygous mutations of the PLS3 gene, and male rats are wild type.

In the offspring of female F0 generation mutant rats, F1 generation female rats have two genotypes of wild type and heterozygous mutant, and F1 generation male rats have two genotypes of hemizygous mutant and wild type. The above F1 generation was cultured for 2-3 weeks, rat genome was extracted as described above, PCR-amplified with KO primer, and then the genotype of F1 generation rat was identified by gel electrophoresis.

2. Results of the experiment

The results of amplification using KO primers for mutant genes are shown in FIG. 3, and show that the edited types of 6 target genes were co-detected, wherein the female mouse heterozygous for the F0 generation PLS3mutation (the genotype is PLS 3)^+/-) The male mouse is numbered 12#, 13#, 26#, F0 generation PLS3 hemizygous mutant (the genotype is PLS 3)^-/0) No.4, No.7 and No. 22, 6 positive F0 generation rats were obtained, of which 3 female rats (genotype Pls 3)^+/-12, 13, 26) and 3 male mice (genotype Pls 3)^-/0And the numbers are 4, 7 and 22), the sizes of the first mouse knockout fragments are shown in a table 4. The results of amplification using KO primers for mutant genes are shown in FIG. 4, in which female mouse progeny No. 26, No. 1-13F 0, female mouse progeny No. 13, No. 14-23F 0, and genotype identification results show male hemizygous mice No.1, 9, and 14F 1 (the genotype is PLS 3)^-/0) The numbers 3, 5, 13, 15 and 21 are heterozygous female mice (the genotype is PLS 3)^+/-) The results show the successful construction of a PLS3 gene targeted mutation rat model.

Sequence listing

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cactccattt tcagtgtaca taggattctg agcctgaaaa ggacacctat gttaaaagca 3060

cacggtcctg atgtgcaggc cagcctgttg cacagattat ctagcaatgg atataaactg 3120

tacctgaact cctagagatg agccccccta gtggaagtaa ggtgacaata taattttaga 3180

gtgaatttct aataaagatt tacctttaaa aaaatagcca aagtgtttct acaactcagc 3240

tctggttaca agtagacact aataaccatt gttaatttcc tagttttaaa agatgtttag 3300

atagatagat ataatgatac tagctaggtg aagatttaaa aaaaaaaact atatgattgt 3360

tgtttttctg tcaagccgaa ttatttcaga ttaaaaatgt tttaaagcca gcatttaagt 3420

ggcagatgca gaggaaccag ggactctagg atatcctcag ctagccttgc ttcagtgaga 3480

gcccatctaa ataaggcaag aagaaaaagg ttttaaattg cttatttttt ttagatattt 3540

agatatggtt ttctttttta atcattgttt aatttttttc atacagtata gttggataat 3600

atttcccccc cagccctccc agatgctgct catctaacta tatgattcct ctctttccca 3660

aaacaaaatg aaaatcaaag aaacaaaaaa caattcaagc aaaacatccc agtgtgaaat 3720

catttctaag aggtggaacc ctcctgaaga gcagggctgc agggggtata agtcatgtca 3780

aaacagaatc atgagcctgg aagatgttca gtgagtaaag gcactaaacc aagcctgaga 3840

gcctgaatta gatccctgta tcacataata gaagttgaga ctttcttatg gggatgcaat 3900

ccccttcagc tacttcagtc ctttaactcc tccaaaggga acctcctcct cagtttagtg 3960

gttgttgtga gcatctccct ctgtatctgt catgctctgg cagagcctct caggagacac 4020

ctatatcagg ctcctgtcag catgcacttc ttggcattag caatgttgtc tggatttgca 4080

gtctgtatat gggttggatt ccccaggtag ggcagtctct ggatggactt tccttcagtc 4140

tctgctccat acattgtctc catattttct cctgtgagta tttttgttct ctctcctaag 4200

aaggactgaa gcatctgcac tttggtcaac ttcaagaagt tcttgggctt catgtggtct 4260

gggaattgtt tcttgggtat tccaagattt tgggctaata tccactgatc agtgagtgca 4320

taccatgtgt gtttttctgt gattgggtta cctcactcag gatggtattt tctagttcat 4380

tccacttgcc tgtgaatttc ataaagtcat tgtttttgat agctgagtag tacaccattg 4440

tgtaaatgta ccacattttc tgtatccatt cctctgttga aaggcatctg ggctctttcc 4500

agcttctggc tattataaat aaggctgcta tgaacatagc ggagcgtgtg tctttgttat 4560

atgttggggc accttttggg tatatgccca agagaggtat agctgggccc tcaggtagta 4620

caatgtccaa ttttctgagg aacctccaga ctgatttcca gagaggttgt accagtctgc 4680

aatcccacca ccaatggagg agtgttcctc tttctccaca cccttgccag catctgctgt 4740

cgcctgagtt tttgaactta gccattctga ctggagtgag gtaaaatctc agggttgttt 4800

tgatttgcat ttccctgatg acttagaatg ttgaacattt ctttaggtgc ttctaggtca 4860

ttcgatgttc ctcagctgag aattctgttt agctctgtac tccatttttt catagggtta 4920

tttgattctc tggagtctaa cttcttgagt tttttgtata tattggatag tagccctcta 4980

tgggctgtag gattgataaa gatcttttcc caatctgttg gttgctgttt tgtcctactg 5040

acagtgtcct ttgccttaca ggagctttac cgagctctca gggactaaac taccatccca 5100

agagtacaca tggacagacc catggctcca tccacatatg tagcagagga tggccttttc 5160

aggcatcaat gggaggagaa gcctctagcc ctggtcaatg caacagtata ggggaatgta 5220

agggcggtta ggtgggaaga agtcagtggg tagctgaggg agcaccttca tagaaggagg 5280

ggcgggggaa gattataatg ggtttctgga gggaaaactg ggagagggga taacatttaa 5340

aatataaatt aaaaaaaatt caataaaagg aaaaaaagaa gttaagacct gacagttatc 5400

atttgacttc catacatgac atggtaagat gtcctgccca ctacacagaa tcattactac 5460

tttctgatac acagcacttc ttggaatgtc cagactctta aaacagctct cattgctgtc 5520

ctgtaattca gcgatattat ctaatttacc cggattttta ttgaagttcg agtattatgg 5580

attttttttt ctcagacagg gtttctctgt gtaaaaagcc cttttggccc taaactctat 5640

ttgttggcta ggctggcctc aaactcaaag agatccacct gcttcttaat ccagagtgct 5700

gagattaaag atatttgcta ccaagcccag cttgtaactc ttagttctta atttctaccc 5760

atagacgtag ctgcttgggg tgcaagctaa attatgttat acctctgttg taatacttta 5820

aatgacattg aagcctaaaa cgtctagtcc ctttgatgag ctttagagtc taattgctat 5880

aatgtgccta ggtttgtggc atttggagta gtattcgtag tattttcaca gaggtaagtc 5940

ttttctacaa acagaaaaag tttatgcctg tattaagctt gacacctatt attagggccc 6000

aatgttcctg tttagtatac tatcagaaac ccagggacct tttcctccta ggcaagtagt 6060

atgactgctg ctgggaaaag ggggagggga cagcttggtc atggtgcttg aacactcaag 6120

tgatgctttt ggacaaggga agaaagtcca cttttttttt tttttcggag ctgaggaccg 6180

aacccagggc cttgcacttg ctaggcaagc gctctaccac tgagctaaat ccccaaccca 6240

gaaagtccac ttttaactcc atgttctgtt taaggtgttg ctccaacatg gcttttcaca 6300

attttcgata tcatattctt agcaaatatt acaatggcag agattcgtat gtatattcag 6360

cccagtatgc tctgggtgga aactagacag acgaagacat ttgttgttgt aactgattct 6420

aaaagtaacg ggtatgaaat cagacttgaa aatgaacatc tgtcaatatc ggcagcttga 6480

atatgtgcct tttagcatta ttttgtactt gttggtatga taggtttttt gatgtaaaaa 6540

gagagcctaa gattagaagt tgtgtcatat ttcgcatctg tgtatttaat ctagggagtg 6600

atgtgtctta taatgtaagc tgaatgcgat ggaggattcc aaagctcttt ggatgcattc 6660

ttctgactgt tttcagtttg gagtcctttg tttaaagttg tggcaactgt ctaatgcttc 6720

cggacaatcc ccatcccatg tgtgtctgtg tctgtatgta ccttaaagag atggacataa 6780

agtgtcttcc tcaatgacct acttccttat tgttgaagac agaaactcta attgaaactg 6840

gaccttgtcc atttgggtag actgactggc cagagagccc tagggatcct cctgtctgca 6900

cctcctcagt gctgtctgtc aggtcaggta tatgctacct cttctgtctt ttgtgcatat 6960

acgctgagaa atctgaacac aggccttcat gcctgcatgg caagcacctc actgcggaac 7020

catctcccca gaacttcaat gccattctta agagccaatc tcaaaataca tgggtcagtt 7080

tattttagtt atagagggga ggcggtttca aaagattcac agctaaattc agtaatattg 7140

aacgtcgcca tattcttacc acatgaaaga aaaatgagag tctggagtat tctagggatg 7200

aagaaatgtt atttaaaaga aaatgctgag agagaagagg ggtgtggcat caaggttctg 7260

taataccaac atatcagatg tcagtgcata gaatgagttc ttagtagcat ggactcttgt 7320

ctcaaaagga aaacaacaaa atatggagag gcaattatgt cagatatagc gtcaaaggaa 7380

ctgctactga gccagaattt catcttgaga tactcacata aaattgttat attggtaaat 7440

aacatttagt ccatatatgt actagacact gagtattttc tcagttttgt aaagtggagt 7500

agctcaatgc ttttagttgg ctggaattac tttttgaata tagctgtaat ctggctattc 7560

tcacttaaat ttcttattgt ttttttggta aatgtgtatg ggtgttttgc ctgcatgtat 7620

gtctgtctat cacttgtagt aactagtgcc catggcagcc agaaaatgat aacaaatccc 7680

tgaaatgtgg agttaaagac ggtcacgagc tgcaaattgg gtgctgggaa tcaaacccaa 7740

gtcctctctc tagaagcctc tagcactctt acccacttga gccatccctc caggccctgt 7800

tctcatttaa cttcctttta tagttgcagg gctggagaac ggatatcaga gtctcatgcc 7860

aggcaagcat tctactactg atcagtatgc ccggcccaca tgttactttt gattttgagt 7920

cagagtcttg cttatgacac ccaaactagc cttaaacaca ctttggagac catgccgacc 7980

tttgaacttg taatcctcct gcctcaacct ctcaaggacc ctaaattata ggcttgcacc 8040

caccaggcct ggctacagac cgattttata ctatgaaaaa cttagaaatg tgattgatga 8100

taaatgtagt gtcgctgagt ctaaacgata ttgggcgtgg aatctctgct ttcatttttc 8160

tgctgtctgg acggattggc ccttttctgt aaatcactgt tgtgctcatg gctggagttt 8220

gcttgatttt taaggcatct gtttttaatc tatgcatttc acaggcatgc tgaaaggata 8280

agtgaactat gaacgcttgg ctcttcggca gacaggtgct atataaatcc agcatactgt 8340

agctgtattt cataactctg ctaggccttt aacaggcttt tttagaagac tgggttatag 8400

ttcaatagca gtacatttgt tttgctcgca tgaggctatg ggttcaatcc tcagcatcac 8460

acatgcgcgc gcgcacacac acacgcacgt gcgctcatga atgcatatgc acaagcacag 8520

gtacacatac actggcagta ttatgtttct taattcagat gttatggaga atagcatgtt 8580

gatgtcatca attgattttt tttccttgtt actaaataat acgtttattt ttccttattg 8640

gttgtcaata cagagtccgt tgtcatcttc cttttaaatt ggtcattagc atacacatat 8700

ttaggtttca ctgtgttatt ttcaaacagt ttgttatggt ttattttcat atacctccct 8760

tctcccattt cccccttgta cttttcccca ccctcctgcc tgcttccagc acacatgtac 8820

cagattgctt cctcttcctc aaaacctctt gccacactaa tggtctcatt cctagtttca 8880

taggttccat agcccaggcc cacacttctg cctacttaca tacatataaa cataatatgc 8940

taggttgcac acatgagaga atgtgattct tgtctgaatc tcaaccacct cacttaaaat 9000

aatgctttcc agatccagcc attttcctgc aaatttcatt tttctacatg gctgaataaa 9060

attatccact gtttttgtat tacgtatttt ttatccattc atctgttgat ggatatctat 9120

aggctgattc attttccttg ctgttatgaa tagagagcag caataaaaat agatatgtaa 9180

gtatctctgt ggcagtgtac agagtcctta tggtatatgc cagaaatggt atctgaatca 9240

tacggtagtt ctgacttaat ataattttta tatttattta ttctgtatgc gcatgtgtca 9300

tgctgcacat agagatcaga gaacaacctg cagaaggact tcgtctatct tgggggtctt 9360

aggaattgaa ttcagtcatc aggcttgagg gcaaatgctt ttacctgctg agccattttg 9420

ccaggccagt taaatggctt gagaataact gagaatcacg gctagtttga taagagtggg 9480

cgacttttac tatgagaaat catttttaag ttgtgtcctc ttatttcgga cattgtgctt 9540

taaaatattc actaggaaag gcattttgcg taatcctgta acttgtgcat tgacaataga 9600

agattgatta tcttaagtta tgatacatac attgaaaatg aatatataaa cacattatga 9660

ttttatgtaa acctagaaag tccttacatt gtagaaaatg caatggattc tcagccattg 9720

tctctgttta tagctgagtg agttcatgtt ctgccagggc tacacagaga aaccctgtct 9780

tgaccccctc cctccctgaa agaaagaaag aaagaaagaa agaaagaaag aaagaaagag 9840

agaaagagag agagagagag aaagaaagga aggaaggaag aaaagaaaag aaagagggtg 9900

tgggcaaggc tgaagagatg gctcatcagt aaaggagtga tgcttgctgc tcttacctag 9960

gacttacctt ccctcctagc acccacatag ggtggctcac aaccacctgt aattcccgat 10020

tccaagaata taacaccttg ttatggtttt gtgggcaatt acactcatag gcacatactc 10080

tcacacaaac acacatgcac acctaattaa aagtgtgtgt aggtagggag gacaacttgc 10140

tggaatcatg ttctccttcc actgtgtaga tgtcccccga attgaactca ggtcctcagg 10200

attggcagca agcattctta accactgagc cgtctcacta gcccttgttt gttttgttta 10260

atgctattat gaaaatactg tttaatattg attttttcat tgtgatgatg gatgatttaa 10320

gcaaaggttt aggttaaagg gactgctttg cttagctcag aatcctgttt atcattgaat 10380

tcatcctttt atgaggattt gctatccaac agtgtaagaa cttcccttca aaggaaaatt 10440

ggaaatgtgg ctactgatca tatgtggtta catatgacac atgaagctta ccagagtgaa 10500

aggtaatggt caatgttcat gcctggtttg ttatatagga caggaggctt caagatttgg 10560

gtggttttca caataataga aatatcaggg ctggagagag ggcttagctt ttaagagcac 10620

tgactgctct tccagaggtc ctgagttcaa ttccccgcaa ccacatggtg tctcacaacc 10680

atctgtaatg ggatctgatg ccctcttctg gtgtgtctga agacagctat aatgtgctca 10740

tgtacataaa ataaataaat cttaaaaaga gaaaaatgaa atatcaatgg taagaaattt 10800

actttggagt aaaatagcat aaacaaaata aattagtgaa aggtgaaatg ttgacaaatg 10860

cagagggtta taggttaaat cagcatctct cctttaaaac atatttatta gtgtgtgttt 10920

gtgattgttc gtgggcagag ggtagaggaa acctctcagg agttggtttc cttccttcca 10980

ctgtagattc cgggttcagg tcatcatggc tgccataagc acatagttta acaagtcttg 11040

gtatggatcc taaatcagga tcatttccaa cgcgacatat agcccttaca aactgataaa 11100

cttacattca acttacattt gataattttt ttccctctgg tagaagattt caaacgtaaa 11160

acccagcaaa tgaaaggtat tgaaagattc gaaaatattc taaacaagct gagcatagtg 11220

gcgcatgctt ttaatcccag cacttgggag acagagctag gcagatctct gtgagttcaa 11280

ggttagcctg gtctccatag taagttccag gacagccagg tttatgggga gataccctgt 11340

ctcacaaaaa aaattaaata aataaaaaca taacaaaaag caagaaacaa aaccaaccaa 11400

ataaacagaa aggttttaag ggatttgtgg agctatggct gaaccctttt tgttagtgat 11460

tctaggggat actccttttt ctgagtatgt atgtttctgg tgatttgtgt tcgctaaaca 11520

aactgcctag gattgagcca caattccatg ccttatttct tgtctattcc ctcacatttt 11580

gagagaagac ttcgctatgt tacttgggct ggactgtaaa ttttgatcct actgcttcag 11640

tctctggagt agccaggatt acaagcataa gttaccagaa gacttggcta agtaattaca 11700

ttttactatt tatcctacgg tgattttaaa atacaaccaa cttagcaact tagtaataaa 11760

gttttaaaag ttctttcaat gggttacgag ttcaaaagta caagtcaatt cacgcatttt 11820

ttttaagtct aaactctttt tttttataaa tttatttttt aagattttat tttattgtat 11880

gagtacactg tagctgcctt cagacacacc agaagagggc atcagatctc attacagatg 11940

gttgtgagcc accatgtggt tgctgggatt tgaactcagg acctcaggaa gagcagttgg 12000

tgctcttaac cactgagcca tctctccagc agccccttaa gtctaaactc ttaagtactc 12060

cagcttctgg tagctctcag atgaaaagca ggctaaaggc aagtgaaacc ttcccaaact 12120

agagctctgt ataaagatga aggaagtttg gtatatctca taccttgaag tgtatatgta 12180

ctggagaggg ggaagtaagg tatatttcag ttggtagaga gcttgtctag catacaatag 12240

gtccaaagtt tggccccaag cacaacaagg cccaagcata gtggcacaag catgtaaatc 12300

tcagtgctca ggaggccgaa tgaggaggac cagaagttca agattaagtt tgaagctagc 12360

cttcaaactt cttcgtagcg gcccatcggc tactaatgaa tagatagaaa cctgtgcaag 12420

tgtccggtgt agatgcctag agcccacaga ggtcaaaaga gggctctggt ctctctggaa 12480

ctggagttac tgctagtttt aagctgccac acggatgttg ggtcatttgc aagaggagtc 12540

agtgctgaac catctctcca gcctcatcat ttaatacaag tatttaatga agactatgta 12600

ctagggttac aaagaggata gtagatagct cctgcaattt aggagaagaa aaagacacaa 12660

aagtaatact gttttttatt atggaatcta aagatatttg ttagaattag ttattaattt 12720

gtgtgtgtgt gtgtgtgtgc atgtgcccaa gcgcaccggc atgtaacatc aggaaaactt 12780

gtgaaaactg gtttttgcct ccaccttatt gagacagctt cttgctactg catagcttct 12840

gccagcccat ctggaagatg ctaacgcaat tctcctctcc acttcccatt tccgtggagg 12900

agtgatggga tttagatgcc cacatgagac ttttcttacc ttggttccag gattgaattt 12960

gggtcaatgc tttcatagct ctcatctcct cctctgcaat atatatatca tggagaaaag 13020

aatttaaatt ccgtcttttt tttaacataa aataatttct aaatttaaac tctgtatgaa 13080

caaagacatt cacttatatc ccagtataat caaatctaaa gtaagaacaa atcacattta 13140

aacattgtta tttctttgca ataattatta cgtcctaaaa aaggagccca ctatgagtta 13200

attttactta aatagaacat tgctcagcct ataaatgagg gtcagcagac accagtgtct 13260

tggaataaaa tagccctttg gctagaaaat gggctacacc caattttgtt tttacaaaaa 13320

taaaattctt tccagcttca ctaaattgga gatgcagtgg acaacgacag tagtagttga 13380

ctttgcgact gtggaaatat tcaagtatcc gtgtgtacag aaacccctag aaaagctgac 13440

ttccacaagc ggtactcagg ccttctgtag ctcctcttca cataggtgtt atcatagccg 13500

tgtataaagt aactcatagc tgatggcttt catttcctaa cttgtacttc atgattttaa 13560

caggccttaa attgagtagg agaagctggg atttcaggtc ggacagttgc tccctctgga 13620

agcggggaat caaagtaata cataatgcat atggctttct tgaaaggcag tgagtaattc 13680

ctgccaatta tgaaccacca caagcaggga ttgaacaaat aaacttgagg ggaaaatccc 13740

tgttctggtt ccttcatagt agcctgacac acagcattcc ctctattttc acaaggaatg 13800

gtgggatggt ttgtatatgt ttgacccagg gagtgccagt attagaaggt gtggccttgt 13860

tagagtaagt gtgtcactgt gggtgtgggc tttaatatcc tagtcctagc tgcctggaag 13920

ccagtatttt gctagcagcc ttcagatgaa gacatagaac gctcacctcc tgcagcatgt 13980

ctgcctgggg gctgccatgt tcccaccttg atgataatgg actgaacctc tgaacctgta 14040

agccagcccc aattacatgt tgtccttata agagttgcct tggtcatggt gtctcttcac 14100

agcagttaaa acctaacaaa gacaaatggg tttcaggaac tctgtcagga tgcagaagcc 14160

tcttatagaa aataaggtgg tctttgcata tagcctatgc tcattcttta tgcttttttg 14220

tgagtggagg tgaatgctgg accaaaagcc tctcacattc taaacttatt gagctccaca 14280

ttcacagcac tctcccacat agatgattaa atcatcccta ggttactcaa cacctcgtag 14340

aatatgaaag cttggcctgt attgcttaga gaatggggac aagaaaagat cgatcaagtc 14400

caatccagtc acgggtcttt ctgaatgttt tccagctggt gctgcttgaa ccccagtgga 14460

gcacagagat agagagggcc cattgagttg ggaaaaaaga aatgaccttg aattgtcttg 14520

ctgataagat gattcatgaa gctgagggct gtagataggg ctcagtagtc tgtactgggc 14580

ataagtaagg tcttgtattt gatccccagc agaaagaaag gggagccaag gaaggaggag 14640

gaaaggatgg caggaaggaa agaaggaagg aaagaaaaaa tgtaaaggcg gggtggaaga 14700

caatcgaatt gaagaaaccc aaattagcgg gccattgttt tatgacacat ggacttcatg 14760

tgaagagagt catatggtgt tcttgctttc atatactttt tagctatttt ctttacttac 14820

tagaactttc attagcataa gttcactgca caaaatggaa gaattcttca tgaaaagttc 14880

atgcaaatac ataatgtact ttgaccatat ttgctccatt accctctctt ttcctcagcc 14940

ccagcgttcc cacaggtcta tttagtcctt ccaaatactc ctctgagatt cacatcctac 15000

atgtgtggca tatgcaggtg tttgcatgta taatgatgct gcatatgaaa gagaactcat 15060

aatatctgtc ctttcaaatg tggcttattt cttttaatat gatccacagt ttcatcattt 15120

ttcctgtaaa tgacacaatt ttattcatct ttgtggctgg aaaaaacatc atcgtggaca 15180

ttgttcacag aactaggggg aaaaaaagtc ctaaaattca taggaaaatg caacacacca 15240

tgtgtttcca aagcaatgct aagcagaaag aaccctgctg gaagctatca aaattacttg 15300

acctcaaatt gtactacaga gctgtactgg caaaaaaaat aacgtggtcc agagaaaaac 15360

agacatgtag accaaagata gaatggggac ctatgaaaga acacagacag ctaggatcac 15420

ataatactgt gcagaggtga taaaagaaaa ggtatacact agaaataaat cctattcaac 15480

aaattgtgct atcaaaactg gatatccctc tacagaaccg taacattaga gccacacctt 15540

tccccttaac caaaaataaa ttcaaattta gttaaaaaga aagaaagaaa gaaaggaaaa 15600

aaaccctctc gttttaaaac atgaaacact gaaattgcta gaggaaaaca cttcaagatg 15660

ccaacataga caaaaccttt tttcctgttc tttgacttag gactcacgta gcacaggata 15720

gtatcaagct cactctagct gagcataagg ttgaacttgt ggttacatca cctctacctc 15780

ttgtgtgctg cgattactgg cttgcactcc cacagtgggt gtagactgtg ctgccgaatg 15840

aaaccacagc ttcattccta ccacgaaagc attctaccaa gtgccgtgca ttcaggctag 15900

tctcaaagtc ttccttcctg cctcaggctt ctgagtgctg gaattacagg cattttatca 15960

ccttacctgg ctttggctat ctcagaggat tgattacgag tgaatgttat gtgctttgtt 16020

ttgttccttc ttttggagat aaagtctctc actctgtaac cacatgctgg cctggaattt 16080

gctatactgc cctgcctgtc ctctacttta cagtttacct gccttagcct cctgcatgct 16140

tgagggtgta agcacggtcc atcacctcca gctatgaata gcatctcctt tcctttttaa 16200

agatgtattt atttactgga tgaatgcttt atctgtgtgt acatatgtac accgtgtgtg 16260

cctggtgcct atggaaatta gaagggggca tcagattcct tgggactgga gttgcccaaa 16320

gtgggcgctg ggaaccaaaa cgaagtcctc tgcaagaaaa aagtgctctt aacttctgag 16380

ccatctctcc atactccagt atgcatggca ttttcatgct aagaactgct gaaccttagc 16440

ctcctgcatg cttgagggtg taagcacggt ccatcacctc cagctatgaa tagcatctcc 16500

tttccttttt aaagatgtat ttatttactg gatgaatgct ttatctgtgt gtacatatgt 16560

acaccgtgtg tgcctggtgc ctatggaaat tagaaggggg catcagattc cttgggactg 16620

gagttgccca aagtgggcgc tgggaaccaa aacgaagtcc tctgcaagaa aaaagtgctc 16680

ttaacttctg agccatctct ccatactcca gtatgcatgg cattttcatg ctaagaactg 16740

ctgaacttta gaatcaacta ggaagctgtg atatgttact attttcacat cgtagtagct 16800

ttagtggcaa actgcatgta tatgtccata atagctttta caagtataca cttcaacaca 16860

ttttagtcca tctcttatta tgtatacgat tatagtccgt gttcttcttt taagatttat 16920

ttattttgtt ttatgcatat gagtcctttg ctctcatgta tgtctgggta acatatgcat 16980

gccaggtctc caaagaggtc agaggaggct gttgggtccc ctggaaccag aattaggtac 17040

agttgtgagc tgcatattag tgctgggaat caaacctatg tcctctgtaa aaacaagtgc 17100

tcttaactgc taagccaatt ctccatccgc ctacagtcca ctttcataca tttgtattag 17160

ctcagacaga aatactgcac tacctaccca taatcttacc accctgctaa cccgtgacta 17220

ctaattgact actaattgac tttgcatctc cataaatttg cctcttctgg atttttatgt 17280

ggattcaacg ctattacata tggtcttttt cgcatctgac ttctacttga catggtgctt 17340

taaaaggtca ccgacattct agcatcagca cttcattgct ttttttatgc cctatcaaca 17400

tttaggttac atagacctgt ccaccattca ttccctcatt ggcggtcatt tgaattgtct 17460

ctacattatg gctgttataa acggtggaac acatcagtgt taaaagttta acatcaggca 17520

agataatgag ttaaaggttg tactgataag attgtctcat ttagtaagct gttaatgaga 17580

atcctagctt ggactgactt tggctgttgc tttcttggtg ctacccactg gtttatctaa 17640

gagttactcc cagtacattt ttccttttcc ttgagtaatg caaagtacat acaagatttt 17700

agtttggttt gggtggggtt agcccggcca tgaacattta tcctcttgac tctgcccctt 17760

aagtagtggg gattccaaac ctgtgctctc agtcctttaa aacaagctgt attatagatg 17820

tttagaatgt tcagttacat atctgttctt gttgtttggt ttggaagatg gcccctagat 17880

gcagatacgg ctggcctcaa actcaaggcc tttctgcttc accttccccg gtaacaccaa 17940

cacacccagc cacaaatttg tttcagtaca ttcttgacta cctaagagca aagataagca 18000

atgttaagga atgcagaagt aaacagtaaa cactctcgaa aacaatggtt atggttaacc 18060

taagcagtta ctgaatacat ttatttttca actgtgctca catagaacaa atttcaaatg 18120

ctacaaaggg ctgtagtata taataaaaag cttccaacac aacatgttca aaaaacattt 18180

gtgctttgtg gttaggaagc acaatggtaa aagggctctg ctgacgtttt aggagtgaat 18240

atgaaaacgg gatgtctata agcactttca agttagaatg tctcagggtc acccacaaag 18300

catcaggtaa aaccaggtga taacaaagtg ggatgggcca ccctggaaac ccagcacttt 18360

gaagctaaag caggaggatt gtgacaagtt tgaggacaaa gtgggagatt aggaggtcct 18420

atctcaagaa cccttaaagg aattaagaag agaactactg gtccctaaaa tcctattttg 18480

ttcctttatt ctggattcaa ctgtaagaaa atttcctgag aagtttttta aaagagtcct 18540

tgtgataaag atgctgaagg gctgctcaca ggtgacagct tctggcagga gctatggtgg 18600

ggaagaacgc agttatcgtt taggggctgg ccattgggag tttgtccatg cttcagtatg 18660

gatgagcaac acaaattgga cttggtgtat tttgggggtg ggtggggtgg ggtggggctg 18720

gggaatgggt caggggtgaa atgatacctg ggaggaatgg ggcgtgaatg tgactggggt 18780

gtattgtatg aaattcccaa atacttacta aaaatatgtt ggagaaaaaa atgtcctgga 18840

tcccagctgt ttactcctct agacacggag aactatgcag gcccttgagg aaaggagggg 18900

tttcactgta caaagaaggc tggaagtcag taccatttcc tggagaccca ttttgaatgc 18960

cacatataat tacttaattc ttgtaacccg tctttgttaa ttgtctacag tgtgcctgca 19020

acactaagat taatcaaaca atatgccacc atttaagccc ggagtccagt cgcggcactg 19080

ctattacaag tgaggcaatg gaggtctgac ggtggttagc ctcagcatac agttgttaag 19140

gtcattggtg gaggagccca gtttatcatg ggagatctgg attttagcag tagacctact 19200

ccattactgt tctctgtcca tttcaacacc acatccatgg tctacactgt ctgatagccc 19260

tgcctcttcc atcgggagtc agttaatact aactgccgtt ttctattaga tactaacaga 19320

ttgacaagtg ctatgccatg tttgtaaact gacgtggaac ctcatgtgcc tgtatgttct 19380

atatgctccc tagaatccct ccacttagca tgaaaacaac ttgggtcaga caattgatcc 19440

atagttaata gaaatgcctt actagttaga caccgagcag ggaactcttt tcctgtgttc 19500

tatttttgat tgatagttct cagtttgcta aaaagcacat attcataatc ctacatggtt 19560

aatgcattcc aaagggttgt aaaactctaa ttcaatatat tgcatgtata tcatagactt 19620

tttaatcact catgatttct actaaattag aatttattgc caggtggtag tggcatatgt 19680

atgcctttca ttccagcacc caggagacag aagcaggcag atttcttgat ttcagcctgg 19740

actacagaac tagttccagt gccgccaggg ctacacagag aaatcctgtc acaaaagaaa 19800

attattatgc acctttttct cctccctctc cccctcttcc ctcctcctcc ccctcacttc 19860

tcctcccccc tcctctaccc cgtccttttt tgagagaggc tctcaggtat cctatgcggc 19920

catgtgtgat cttgacctca tgtgcctgta tgttctatat gctgagatta caggcacaga 19980

catatccagt gcttaaggta ttggagattg agtccgaggc ttcatataaa tactgggcaa 20040

atactctacc aactgagaca aattcccaga ccgtcactat cgattcctga tcatctgctt 20100

cagcttccca agtgctggga ttacagggct ttgccattac acacagcccc tgtgttacac 20160

ttgaccaaga agaagtttca ttcaacaaat cattcgctta ttcaaataat tactccataa 20220

cttacataag tcaggtaata ttttgtcatt agggatcatt gttgtgaatg aagttccaaa 20280

gggcaccact tttatgaaac ttaacatact actagaggtg gcatagaagg taacattggt 20340

aaagtattgc tcttgtgaca aatagaaaaa tgatttaaat ttatttagag aaactagttt 20400

tggcccatgg tttcagtcca aggtaagctg gctgcattgc tctggacttg agatagggca 20460

gaatatcatg ggagtagtcc ttgtgaatgg gtttactcac tacatgacaa tcaggaaggg 20520

gagaaagaga ccagaaattg tgtagtaccc taaaggacat gtccccattg acctgttcct 20580

tcccagtaat gccatgagat tattaattca tcaatggttg tatccagtga tcaaatctag 20640

gaccttatgg gccaatcagt tctcagtgat tagctgcact attgggaaac aaatcttcaa 20700

caattgagcc ttttgtagaa tttattgtgg ttatggtgtg tgtgcatgca catgcgtgtg 20760

tgcgtgcgtg catgtgaata agcatgtatg tatggtgtct tccttggttt ctttcacttg 20820

ttttctgttt taagacagag tctctcacta agcctgaagc tcattgattc tcctggctgg 20880

ctagtccaca aagcttagga atcgttcaat ctccacccct tccccaacaa cactagagtt 20940

acaagctagt gtaaagcacc accatgcctt tatatgtgtg ttgaggatcc aaggtcgggt 21000

tctcatactt gaatgaccat caattttgca actgtgacag ctccccagcc tcttgtggga 21060

cttttcatat ccaaacctaa catgtagtat gttagataat tcccctatct tccttcaaac 21120

cccctacttt tctcactttt attttgaaac actcccacac tataacccaa gctgagcttg 21180

cttgaacttg tgatcatcca gcctcagctc ttggtcaggc atgggctacc aagcctgact 21240

gggaagtgtc tttattcatt ccgtgtatta ttctgatgag tcttccagaa tattgtgcat 21300

atccattctt tgttcctttc attactaaat ttcagtctgt ggtgagaatg tacgtatgac 21360

tgtttaacaa tggccccatt agagcacatc tggaccattt ccagctgtat cttgctattg 21420

ctttaaaaat aaagtttcaa catccccaca ccctgagttt ctgatacatg acttgttttg 21480

cacaatcgcc catgcctgga agttgcaagt attactttat ttattggagg gtggcatgca 21540

tatgctaaag cacccaaatg gaggtcagat aacaatttgc aagacttggt tctctccttg 21600

caccatatgg ctccagggat ttaaactgac gtcatgatgc tggggcttca acttttgact 21660

ccaagcatgc taagcctatg ccctaccagt gagctatact ctcctctcat ttccagtttt 21720

gttgttgttg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct 21780

gccacagata aaggaggcac tataaacatt tgtgtgcaat cacttgtcca agcgtaaggt 21840

ttatttttgg aaaaccatta cagtttgaat atgaaatgcc ttgtgctgga tcatacattg 21900

aatactaggt ggttctactt ggaaatttga agaggtagag cccagctgga agacataggt 21960

cactgaggat gtgttgttgg gaactgtatc tttgccctgt gcttctatgt ctctaccgtg 22020

tgtcatgtgc tctgccatac agtgttgcct caccagaagc taaaaatcaa tggaaacaag 22080

acaatggact gggaacctga aatcccgaac caaaataaaa ctttattcac ttaagttgta 22140

tatatcagct attttgtcac tatctaaagt gtgcaggtac tgagttgtat ggaagttgca 22200

tgttagtttt ataaggaatt gccaaagtac atgggtagag tactagactg ttttctactt 22260

tttagaaaag atttgtttat tttatttatg tgagtacact gaagctgtct ccagacacac 22320

cagaagaggg catcagatcc cattacagat ggttgtgagc caccatgtgg ttgctgggat 22380

ttgaactcag gagctttgga ttaacttgat gaaaaattta attgagtatt cagtctgatg 22440

ctaaatctta ttttctaacc ctatgccttt ttaaaagaga ttaaaatgtc atatagccca 22500

ggctatcctt gaattctcta tgtagctgag gatgatgtga acgtttgatc ttcctgcctc 22560

taccactaga gtgctgaaat tacagatgta gtcttctaac tctgctcagt ctgtcagtaa 22620

tgcggactga ccccaggtac tcattcagcc acaacaagca ttctgccagc cgagctacat 22680

accagaaaca tctattttta tagttaaaaa taattaagcc agggatgatg gtacattccc 22740

ttatccccag gactcaggca ggtagatctc cgtgagtttt aggctagccc tagttcagag 22800

atgtttatat acagagacta tatctcaaaa taaattcagt ttaccccttt aactaataca 22860

cagaaagata aaactgcatt tttataatgt accatgtgat ttgacagatg tatgtgctat 22920

ataagtgttg agataaaggt acctctcttt atatttaatg atccagagtg ggcggcacag 22980

aacaaagcca catgactcat ttttccatcc tggttttagt ctcaattgcc ttttattttc 23040

attgaggaaa acttaccact cggcctgtaa cattggaagg tgggattatg gggttagagt 23100

tcgggagcag aatcaggcaa gtcacggttc aaacacacct gctctgagta catagcagaa 23160

ttactgctct atactgacag ctgctttaac gtacatagca catggatcct agggctactt 23220

gtcacagtgc tgactctagt cactggtatc agtgatgtat ataactgctt agggaatggc 23280

tacagcttca gcgaaacctg tccttagcaa aagaaagtat aatctgggaa agaaagggaa 23340

gcgtagcagt tcgcacctct agtgtgggga gagtgagtga gacaggagga tcacaagatg 23400

aggcctgttt gggtgtctat ggaaagttca aggctggcct ggttggcttc atacttgaga 23460

ctctgtctca aaaaaaaatc aaaataagaa aacaaaggag aaaaaggatt gcatgtggta 23520

tagttcagtg gttgagtact tgcctggtga gtaacagcta caaagcttgg ttgtcttttt 23580

aaagaagcaa tgatatgata tgcatcaaaa cagatattca agtttctttt ttcacatgta 23640

attgatttgg ggtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgagtgagtg 23700

tgtgtgtgtg tgtgtgtgtg tgttttactc cagggcacat gttagtcaag cacacttacc 23760

actgagtacc actagccttt aattttgtct tctaaacatt caccctggaa attcatgaat 23820

tctaaaaacc agtcgacata caataatcga ccacagtgga agagctccga agagcttagc 23880

caatttctga agtaatgagc tgggagcagg cagacgaaga ttaatcctaa aaatggaatg 23940

tttagcaatt gaagacgata gggcctttga cttgtggcaa aattcattga aatgagatgt 24000

gtttagccat acaaagttaa caaatcacca agtgacgtat cggcccagca aatcttcctg 24060

attacagtcc atctctttag caagctgaac cagcctgtta ttgtcttgat gaattctgct 24120

acattctcaa agatacacaa tcaagcttct cagcaaaaat aaactaaata aaatttcgtc 24180

atcttttata tgtatatatg aaggttaaga gcacgtataa ccacttttag agactgtgga 24240

attttttggg ggcaatcttg cagataaatc tacattgttt tcacagttct gtgtagaggc 24300

agatttaaca atgaccatta tgtgtagaaa atagaaacca ttgttgctgt gctaaactca 24360

ctggagatta aaaaaaaatt gaacgaagca aattaaaacc ttaacggtaa cacataaaag 24420

aaatcaacca gtaaagagtg attagaaaca atatagaagt actgttgaat tagacgtggc 24480

aaaagatcgc tgaagtgtat agaacgctga gaatcactta cttatttaaa aagataattc 24540

caaaactgca tgcacgcaca cacacacaca cacacacaca cacacacaca cacacacaca 24600

caggcacacg aaatttgggt ctttcagcta ttattttgga gaaggcatga gaaaaatata 24660

ttcagaagag ataacaatgc agtctgtgca ctgccaaatc ataatgacat ggaatttatt 24720

tggtttgtgc tttgacagtg tgtatatgtt tacaagaatt acgagctagc attgagctga 24780

tactgtgcag ttcttaaagt gaatgtcggc aatgttctga atgttttatt agacaatgaa 24840

gccacaagtt ttctctgtga acttgtctct gcaagggtag cagaagacag actatattcc 24900

ccatgcaaaa tatgtgaaac ttcatggatg cattcaacaa cctgaaacca tggcaacaag 24960

tggcaattga cgtcctagaa gctctggctt catgttacca cttcatctga attagaagtt 25020

taaagagtat tttatatgcc ttgctaaaaa ctgacaagct taatcttcgt ccagggcatg 25080

caggcaggaa atgctaaaac agctctttct tttgaagatt tctttcatta ttccctgtga 25140

ggtcaaaaag cagttgtgag tttggaaacc cagagcagtg atgtcttgag agctaaatga 25200

gatttttctt tcttgtaggg cgagtttggt ttgttcagtc ctccttgtgt gccaggcact 25260

gtgttagatg ccaaaataga ttactaagag aaggagacaa gaaaaatcat tgccgttata 25320

ttctttgatc atggttgagg ctctagaaca atttccagtt atttaatatc attcctcagc 25380

ttttaaaagt ctcccccaaa agagtcttga actcttgaat attttatctg atttgaatag 25440

ggagaactga tgatttgttt ggaattcatg gcaagaatcc aaacgctcat accgaaaata 25500

aatattttct ttttagtcac caaaaataaa tattttcttt ttggtcgtca ctgctgtgtt 25560

tcctatgcca gagtcacacc tgcactgctg actgacctag gaatgcttta ggcttaactt 25620

gtggattcag tctttattcg gggcttaatt ccaattctgt gggggaggga aaggatacag 25680

gaggctcaat gtaatttgta aattcattta gaaatgtgtc tctgtcgtgt gaagatttat 25740

ggaatcctca aactggactg aacagtcacc acggaaaatg aatatatcgc ggagttattt 25800

tcgcccgggt gaggagtaca agggctgact caaagaggat gccatgacgg ttcctgtgct 25860

gaagttgaat aagatgacag gctgagctgg aaaatccgtg cgctcatgcc caagtttgct 25920

cacatccggt gttcttttcc ttttatagag aaaaatcaag gagagtcagt caagtccagt 25980

ctctctctct gtcctctctg tcttctctct ctcccccccc aatactttct cctgccattt 26040

tttttatttc cttataaaat tccattttaa taatttgcat ttggttagct taaattgtaa 26100

taacacgaat aagaacctta gcatagaaga ggaatgatac ccattctttg gaattcaagg 26160

ttaataaata tgttttttgg tttgtttgtt gggagacaga ctggtcttga attcattgta 26220

taggcaaagg taagattaaa tttgtacttc tgactctcct gcctccacct ccagcattct 26280

gtttactttt tagacagaaa cttgcactgt agtccaggct aatgtggaat tcactatata 26340

gccatactgg cttaacactt ggacagtctt cctgatttag ccttctaaat gctaggatta 26400

acagacagat ataaactacc ctacctggct caatacatat ggttttctga tgacagtgat 26460

gatttttaat taatatgttt ttaggtatta tacaaatgtc agcatttaaa agttcggctt 26520

aacttggtaa aatggtcatt tttccagtca ttaatgcata attaatgaaa ccaactattg 26580

gctaaaagaa agttccactc aaaatgcaac tgaaacacca aagggattta attgaaaagg 26640

tatggccaac acaattttca acttggcttt tggaaataat gtttttttaa atatgctata 26700

cacatttcca tatgataggc tatattgtgc ttttcaagtc ttaacatttt tcccaacagc 26760

aaatttaacg tgatgattgc attatttaat tcacatatca gaaagttctt ttttcaggtc 26820

caaagccaaa aagttggggg aaccattaag ttgactggaa tttgctgatt tttccttctc 26880

tttttttttt ttttttttct tttttttttt tttgattttt tttttgaatt tttttttttt 26940

attaacttga gtatttcctt atatatcatt tcgagtgtta ttcttttccc ggtttccggg 27000

caaacaatcc cctcacccct cgggcttcct tatgggtgtt ctccccaacc ctcccccatt 27060

gccgcccctc cccccataga ctattttcac tggggggcgg attcagtctt agcagggacc 27120

caggctcccc ttccaactgt gctgctttac taggatattc ttgctaccta atggtccaag 27180

tccagggtcg tccatgtatg tgctttagga gtggcttagt cccctggaag ctctggttgc 27240

ttggcaattg ttgtattttg gggtctcgag cccttcaagc tcttccagtt ctttctctag 27300

attccttcaa taggggacct attctcagtc atggtttggc tgctggcatt cgcctctata 27360

ttgctgtatt ctggctgtgt ctctcaggag cgatctacat ccggctccct gtcggtctgc 27420

acttctttgc ttcatccatc ttgtctaatt ggtggctgta ttctccttct ctttgttttt 27480

ctatccctca gctacttaac ccccttcctg ttccttcttc ctcttcttcc cctcccccct 27540

cttcttctct cctcctcccc cctcttcctc ctggtctttc aagcaaaatc atgaaggttg 27600

aaagaaagca tgacttattt ctgtagatgt tctcattagc tgtgaagaga acacagattc 27660

aaatcagact ctgttctaca tggagctcag ctcttctagc cactttctga gaacttagtc 27720

ttcaaataga gaaaagccag aaatggtgag ccacacctgt aatcccaggg ctcaggaggc 27780

tgaaacagga ggatcatgca tttaaggcac acctctaagg caagactgtc cataacttaa 27840

aattcacata gaatcctggg ttagtttaaa gcacattatg ttattattga aacagagctg 27900

ataaatacct gacaatatgg agtcatcctt ggactgctga gattaagggg tctttggtat 27960

atttttttta tttacacttt aaatgttatc cccattccca gtcccctctc tcccagaaac 28020

ctgctgtccc attcccctcg tccctccttc tatgagggtg ctctctcact cacccaccca 28080

ctccctactg cctccctgcc ctggcattcc cgtacactgg ggcatcaaac cttcaaagga 28140

ccaagggcct ctcctcccat tgatgcctga caaggccatc ctctgctaca tatgcagctg 28200

aagccatgca cccctcccca ccatgtgtac actttggttg gtggtttagt ccctgagagc 28260

tctggggtgt cttgttggtt gatattgttg ttctttctat gaagttgcaa accccttcag 28320

ttgctacttt agtccgttct ctaactcctc cattagggat cccatttcag ttccattgtt 28380

agctgcgagc atccacctct gtatttgtca ggctctggca aagcctctca agggatatcc 28440

atatcaggct ccagtcagtg tgcacttctt ggcatctgca atattttctg ggtttggtga 28500

ctgtagatgg gctggatctc caggtggggc agtctctgca tggcctgaac ttcagtctct 28560

gctccacact ttgtctctgt atttcctcct atgaatattt ttgttccccc ttctgcaaag 28620

gactggagca tctgcacttt ggtcgtccgt cttcttgagc ctcttgtggt ttgtgaattg 28680

tatcttgggt attctgagct ttgattaaga ggtttgtata cctttattaa ttctttttaa 28740

gacttattta tttcatgtat gtgagtacac tgtagctgtc ttcggacaca ccacaagagg 28800

gcatcagatc tcattataga tggttgtgag acaccatgtg gttgctggga attgaattca 28860

ggacctctgg aagaatagtc tgtgctctta actgctgagt catctctcca cccccatctt 28920

tagtaattcg ttgagaatca tacatcatac agtgtattat gaccatattc atccctaact 28980

gtatctttat ctcctgccag atacagcccc accactgtac atccacaaat gtttgtcttc 29040

tgaaattaag atattgttct gtaccatttt tctccttaaa tatgaaaaaa cattccaggg 29100

actatagctg taggtcagtg tgaaagcatt tcccagcatt cataaggtcc taagttcctc 29160

ccaagtactg gccttacagg cttatagcac ctcctctgtc tacccctccc taattacctt 29220

catattaaag tttttttctt tgatcttcct ggcctttgaa tgatgtggat tgttccacct 29280

cctcccatta aataaattat agtatcaatc taaaatgcaa ggtcgtgttg ttacggcatt 29340

tgactgggtt ttttgttgtt ggtggtggtt gttgttgttt tgttttgttt tgcttttgct 29400

tttgctttcc tttttttgtg gtatttgtcc cttcatttgg attggaccta gaatcttaga 29460

catgttaggc aagtggtcta ccactgggct acaaccacaa ccattttatt tttaaatact 29520

tttatttttg aaattaaagt atagttatca ttcctcacat tccctttact cacatcaagc 29580

cttccaatgt acctgtctta ctatcaaatt catggccatt gttttttcaa ttgtcggtgt 29640

tgttttgtat atatttctta atatataaat atacctgatt gctctggata atgttgcttg 29700

tatgtatctg atttcagggc tgaccactgg gtataagata accaatgggt gctctcttcc 29760

ctgagataga acatgctccc actttcagaa ttccatagtt gcattttaaa aacgtttgtg 29820

tgttaacata actaagtagg aatctgaagg tgtcatgcag taaactggtt tttatattag 29880

ttggggaaag gtttttttgg gttaggctag ataggaattg agcacagtgg tataatatgt 29940

acaacctaat actttcaatt tttaaatata attgaccagg tcactatttc ctgtgccctg 30000

gatagaaaaa tgaattagat taagtaagca ttgagcatga agagagaaag atatgtaatc 30060

atcagaatgc ttgaagtctt tgcatcgttt atgccgccgt ctgttatatt ctattggaga 30120

tatgaatgaa aagaaaaatt atttttcaaa tgaccagtgt tgtagatgca ccaatcaaat 30180

tatcttcaat aacaacaaaa tttctttgtg tgcctgtgtg tgtattttgt gtatgtgcac 30240

aggtgagtgc ccccaagaag gaactggatc ctccagagtt agaattagag ctctacatgg 30300

gtgttaagga ccaacgcagt ccccaggaag agcaggaagt gctcttagtt actgaaccat 30360

ctcaccagcc ccctcaaaca ctttattttg agacactatg atattatcca ggctggcaca 30420

accttgtgat cctcatgcct cattacccct cgatgcctac atatctaact tcatcctcag 30480

taactaggaa tttaggtatg tgccaccaca tacgaattgt ttcatctttg tctttttggt 30540

ttggtttggt tttggtttgt tagtttttct tgtgtttaga cctgttgtaa cactgggcat 30600

tgaacttaag atctatttat atactagaca tcttctctat tactaagaga ggtgtttact 30660

atgagacagg gtcttcaata agctacccaa gctacccttg cactcagtcc atatcctatt 30720

cagccttgaa tttgccatat ttctgcctca gcctccccac cacgttcgct ttatttacct 30780

tttgacatca tatcatatgc cgtacagcta accagatatg gaagcaatgt aaagtgttta 30840

cattccaagt tccaatcctc tagtcatgtc tctagaagat aaagtgcatc acaatatgga 30900

atgattactt attggggcat cttgtgctta tgaagtgggc agttactgct tgggatctca 30960

ggatgctgaa ctttttctcc aaatatgaaa gaacaattgc agaaccttat atagctctgt 31020

gtaaatcact tgtgtggtgt gcatttgggg tgagagggag gaaaagcaaa agtaaccctg 31080

aaaatagatc catgtgaatg ttaaaacaac aattgtctgt gcaaccggaa gtttgcatag 31140

ctgctgctgc tgcttctagg atgcctcctt gaaatgttcc aattttcttc aaaaattaca 31200

gtagcattgc tatactactg tgcattatat tggctccagt attatatgtt agacctctgt 31260

tcatgaatgt ttcattcact gtgtgctatt tttttttaaa aaataaaaaa gggtaatgcc 31320

ttgtttcttg aaaggaaatt agatcattct attgaggtgt gtcattaagc agagtgggag 31380

aggtctccaa ggtacccgtg agacacggaa agaatggaca gagcactggg tgtggtgggg 31440

agtatgatgc ttgagcgaaa gttggaagag gtcttaaaaa tatgctctag attttgtttc 31500

tagctacaca tcgcattgct acgtatgtga aactctcctg tgacgttaat gggtgtgcct 31560

ctgattttct ttttctttcc accctcaggg gtgtgtgtga accttattgt tttgattgta 31620

agatctaaag gcaatttgca cgtgttttcc gttggctttg cattcaacct gatgtattgt 31680

gacgggaata tgaaacccgt tttcgttctg ttgcccgctt gtaaaaaaaa aaaaaaaaaa 31740

agtaaggctc atgattcacc cttagccact atcttctact cccaaggcaa ttttcattac 31800

ccagagtaaa aacaggttct agtcacaggc atgggcacat atacagacac agcgtcaccg 31860

tttagcccat cctataccac ccgtttcagg tgctgagtga aaaagaagtt tacgaaatga 31920

accatggacc acaaaataaa aaagaaaaat taaaaatctt gagtgtacta ttgatagtga 31980

agaagtttcg tgtagaaaac ttcgtgttat agggtttttg tttggatttt tgtttttcta 32040

gtgctggaga ataaaactga ggagctcttg aatgcttggt aggaaacaaa cagatcagta 32100

gcatttgagt ttgaaaacat taaagaaata tatgcattta cctatgacgg aagaagtcag 32160

ggatacattt tggttgattg tttattttgc taagacaggg tttcactgtg tagctgacct 32220

ggaacttgct atgtagacta ggctgacctt gatctcagac atccactagc ctctgcctcc 32280

tgagttctgg gattaaagat gtgtgccacc attgcctagc atcgatcgag tttctaagta 32340

gcctgttaga agtatctgta aacaggaaag aaaagtgtat tctcaagcag cacactgttt 32400

aaaacatcat ccagtaatgg ggttgtacat ctgaaaagag tggtgatata gcacttggga 32460

cagacctatc taaggggttg aggcaaacgg tgtcactgta gtacagctcc tgttgggaaa 32520

ctccagcatt tctggcccag cactgctgtc tccttacctc aggtggttgg ttctgttcca 32580

gtattcattg actgcaaaat acaggattcg tctgcctggt ttctaccttc cagttaatac 32640

caggggcttc gcaggtcctc cagaagtatt atctatccta acaggccccg gggagatggt 32700

gctaggtttg ctttgcaaat ggagactttg aaaaagctaa gaaattcata tactgagtca 32760

gcaattgaaa tggattaaaa aaaaatacat cccctggctt ctcctggtac ttcctgtcca 32820

ttttacattg tgttgctttt gggggggagt atttcatatg gactttgatg attgctgtaa 32880

tgcactgaag catgtccatt aagcaatgaa gcgtaatcca gtattggtag gcatgaggta 32940

gggaggagaa cagaacattg tctgcctctt aatgaagact acccagtggt ggttcctctt 33000

cacaagtgaa cgctctgtga gtcacatctg tgtgctgaga acttaatggg ttatataaga 33060

ctcatcgcct cgttttatct tcttcataaa aacccagagt atgctttctc attttatttc 33120

ctcatatggt agacagagga gatgttagta agcaggtgtt atccgggaag gttacaacac 33180

tatccaccac ttgctgaaca ctgaccctct cccatacctt tcccagcctt ctcccatacc 33240

tttcccagcc ttctcccata cctttcccag ccttctttta acctctaaaa gatatgtgga 33300

attcgctcca tttttttcag atagggatgt aggccccaga gaggctaagt gatttgataa 33360

gggtcacaca gacttacaga ctgtgacttc taaggctccc atgctgccat tccattcctc 33420

ccaggttatt catttgcact gaatctagag aaatcttctg agcctagcca gtacagctgt 33480

gttttttggt ttggttggtt ggtttgattt ttgtttgagg ggttttgttt gtttgtttgt 33540

gggttggttt tgttatgttt tttgtttttg ttttgttttt gcttcttttt atctatccat 33600

tcagtaattc agttactaga gtagggaaga agaagggtat taataagaac atctgtccat 33660

aactaatgat tgccatcatc tacatttatg gcgtatacaa aatcattaaa aatgttctac 33720

actgcataag gaaattagtt actctgatat ggagtagttc atatttttat tctttatact 33780

gctacgacta ctaattattc ttttgtataa aatattatta ttattattat tattattatt 33840

attattatta ttattattgg tgttaagaca ggttttcact ctgtagccct ggctgtcctg 33900

gagctctttc tgtagaccaa gctgacctcg aattcacaga gctcctcatg catctgtaac 33960

cccattgctg ggattaaggg catgtaccat tatgcccagt aagtattgca tatcattaca 34020

atcatttgca gggtaagaca tggaacctta gggcctttcg catactgggc cagcactgtc 34080

tgagccccag ccccagtttc agcccaaaat gcttatggtt gttgtcatcc tggctgggaa 34140

agccaggctg aagagattaa acaactggca caggaacaca taaacctatt aaattgtggg 34200

gctgtgaggg atggacaccc agtctgactc tggagcactt tctaaggtgc cactcctttt 34260

tctgacagaa cagtctggcg tactgtgctg ctgtcagagg ttgctcttcc aacagaaacc 34320

aaagtgaaat aagtgtgctg ctccagttag aatctggagt ataaatgttg tatgtggact 34380

ggaatgaata atagaaaaca taagcgtcct gggatagtta atttgcctct ccaagtaatg 34440

tacttgactg tggatgagtg gcttggacac tggggagaag ctgttctttg tgttctaaaa 34500

taagccggtt acttggctac agctcaaact ctaaataggt atatgggttg tagctatttg 34560

acctgaagca acttgaagtt cccttggttc cctgtctggt tttaaaagtg gaaccaaaac 34620

gaaagcataa aagcatcaag tttgctctca aggttgtgct tcagttcaaa tttattccac 34680

ctctggcaaa ggactgcaaa ggcagccagg aaggagattc gtaagtattg tatcatttct 34740

ttgtcagggt gttcatttgc tagcaggaag tgttgtgact tgttctttaa agatgcaatt 34800

gtaagaagaa tgttgggttt ccagatttca agttctgggc gtgggagaca gtcctcttag 34860

tacttcaaga aaagaagcag gttcgctcca gtcagaaggg taactatttt aatattgttt 34920

ttgtcggtgc taacttttta ggttactgaa tatttaagcc gagcttgact gtttaaaaca 34980

tgacgttttt attttctgtt taggtaaacc tttgtaatca cttttaaaat cttgattaag 35040

gataagtcag atttgttgta aaacattttt gagaataaga atttaacctc tactttgttc 35100

tatattctgc tgtttttctc ttcttcaatg ttgggaatgt tctaggggtt ttgttctctt 35160

ttttaaaaaa tgtgtttgaa gtccaactta tgtaatctta acatgtcaag ttggatacct 35220

cacagcttaa ttgatagaaa ttgtatctgt ggcattttga atggaaggga gaagagacag 35280

gatttcaaat gcctttgctt cttgctaaag gatttttttt ttaaattctg taacaaagtt 35340

tgtcatgcta gaacatgtca cgattagatg aggatctctg ctcttacaaa gtggcctatg 35400

tcaaacagtt gtcccagcat ttctagttca caacacacat gatttagatg gtgttcctgt 35460

cccaaagaag tgttcatctt gccagcaggg ttttcaatca tacacagctg aggaacttaa 35520

aagtcttaac ttcctgagtt cctgacaaaa ggaagcatag ctagttatag aatgatctta 35580

aatccgagct aagtaagtct catctatgtg gtctggaaca ttaggtctag tgttttcagt 35640

acagggagaa tatttgaatt ggcaaactat atgatactga agaagtcact ttggtgacaa 35700

agagatacag actatgggta agcgatgtat gctccacgag tttgggaaaa caaacagaga 35760

tgaaagcttt caaaagaggt atttcattcc aaaaaatcag tgcccattag cttggcaaac 35820

aaattgagga acgaaaaggg taatacgagg taagtcttct ggtaattttt tttaattttt 35880

gagattataa tataacatca ttcccctgtc cctttcctcc atcttggttg agtctttttt 35940

ttttgagttt gacatattta aatcactatt atgacttgca ttaaaaatct atacaacttt 36000

tgggcatcaa gcatttaaag aatgtcttca atgtaagtta agtcaaacat tgctattatt 36060

ggcagagtta gtgacttatt tttaaatagc ttttagatcg tatctgtgtt tattaagagc 36120

caaggggaag ggaaaggaaa cttttactta aaatggaagt atttcatgtt ccttcaggaa 36180

gtgacacaga gtcgggcacc ctttgggatt tgagtgactc atacatactg gtactgaaaa 36240

ggatcaatta gaatctagct tatactagtc tagaagagat cgcaaatatt ttcagtagat 36300

gaatttgttt cttgatatgt agtagtgaaa tcatgggatg tctggtgtta gattgaaaac 36360

aattccagtt agagaggtag ggcaaagagg aacaggaagg agtgcagaaa caggggaagt 36420

gtctgtgggg aaactaggag aaatggagta cctgctggtt gtgcggatgg tgattgtata 36480

ttattttctc acagttttat acattgaaaa tgctaatagc acaaaacttg acatataatt 36540

agctaattgt ataactagct atgtctgttt gtaaacatcc agttttacct gggaacggca 36600

agcctgtcaa aacatgatcc atgcccaagt cggattgtaa aggcgaaatg tgctgcatgt 36660

gtgccaaaag gcagcaagga ctgtttctaa acttcttgct gtttgacttt cagtcacatt 36720

cacatcgtag cctgcaaagc tcttgaagag ttctgtggta tcttcaagtg tgaaacctga 36780

gtaaataagt aaaacatgat cacaggctaa ttgcatgtaa agaatgtgac tttgttgtgt 36840

tctcagtttt tttccaaagt gttttgttaa taatgcttgc aactttattc attcattcat 36900

tcattcattc attcattcat tcattcatct gttggcacta aggactgtga ggtctgagcc 36960

tcacatattg ttaatgctag agaaatactc tgccactaac ctgtactctg aatctcctta 37020

gtttagcatt gtttgtttag gctgcatttg tttacttttt tagacagggt ttccttatgt 37080

agcctatcct gtcttcgaac ttttgtgtag cccagggatt cacaatcctc ctgcctcaca 37140

ctcccaagtt ctgtaattat aggtatctcc atgctcagct cacgaatatg cattttgcaa 37200

acccacttga gatgactaga tagactaact tcctgaaagg taggtttgct cgctcccatc 37260

aatgcttatt cctttagaca atttttggtg tgtacttgtt gtgagccaag atacaaaacc 37320

atggcataat cttctctctt ttcaagaagc caggaagaaa taagtgtaag agacagaaaa 37380

ggcagaaggc aatcagctca ctgtggaatt taggaaatgt ttactgactc acacctatga 37440

tcatgtgttg catctccttt aaagttatcc aaacagaaaa tttgaggttt gggcatggta 37500

gtctatggct gtaatcacag aatttgggaa gcagggacaa gagaatcagg agtttaaggc 37560

cagcctaagg tacatgaatc cttattatct caaaacaaaa acaagcaaat ccacaaaatt 37620

ttaggatggt atagttagtt gccagtatta aagaagtaga aagtgggcaa aaatagcata 37680

ggttaaatcg atatacgagt tttcaaacaa acaagtctct gcccctttat tcttctcagg 37740

aatctttaaa ggctctgtag ggatcatgag acaaggcatg caaaggaaag caatatcttt 37800

acatttccac ggagagaggc gtttaagtcc ccttaaaggt tttgaataca tccaagttaa 37860

aattagactt aaaggaagga agttcatggg acagagcaaa gggtaaagga agccttcagc 37920

aacagttgtc cagaatatag caacttattg ggaattgaaa gcatattttg tcaaggaagt 37980

atatgctggc ccttgttaag atttaattat ttaaagctgg agagacagag atgactccat 38040

ggttaagagt gcttgctgct cttctgagga ccttggttca attgccagca gtcatgtgac 38100

tactctaacc atccgaactg atgtccacgt tggcctctgt gagcacacag acatacatgc 38160

atggtaaaaa aaacactcaa aataaaaagt aaatcatttt agaaatatat atttatttat 38220

aatgtgtgtg tgcatgtttt gcttgctact tgatgggtgt gcaccacaca tgtagtgccc 38280

ttggaagcca gaagaggatt tcagattccc tggaactgga gttacagaca tatttcatct 38340

accacagggt gctggggact gagctgaaat cctttaccaa agcagccagc tttttttaaa 38400

ctgctgagtc atatctccag taccaagtaa taagactttt caaattacaa gtatctttga 38460

actcgttatc tgactttttt ttaatattgt cattcacgtg ttgtttggta atacaactga 38520

ttgaatcgag cgtctcgtga atgctaggca acactctacc actggactgc atccccagcc 38580

tcctcttcat gtataatttg gaagcgtaat ctggctaagt tgtgctgatt gctgttgaat 38640

ttggaatccc ttagcttcag cttgcccagt atgtaggatt ataatgctgt tccaccaggc 38700

caaacctatg ctccttaacc agagtgaacc aagaagcgga gctggaaaga tgcttctgta 38760

gttaagagtt cataccgctc ttgcagagga catgatgagt tctatttcca gcgcccacat 38820

taggcagctc ccaagcacct gtaactccac ctgcaagggg agcccatgtc ctctgccttc 38880

catgcgtgtg catgtacacg aatagatgaa tattttcaaa aagaacgaac caagagtact 38940

tgctacatag atccagagtc tttcaagcag tggtttcatt ccccacgttg taattctgta 39000

caaccgaaat aaccgatgta gctatgccta aagctcaaat gctgttggtt ttgtgaagaa 39060

catctaaaga cgttctctgc ttttgtttta cagtgtgtcg ctgaaatgtc aaacataatt 39120

cccagaagtt gtaagcctga aaaataactg tgctaagcaa agtctgttgt ataattgaac 39180

attcctttca tagtttgttg ttgttgttgt tgttgttgct gttgttgttc acattgagta 39240

tgaaaaaacc tggatattta ctataaaaaa caattggcaa gaccaggtat ggtggtaaag 39300

acctttaatc ccaacactta gaagctgagg caggtgaata tttgtgactt ccagaccagc 39360

ctggtctaga tagtgacatc ttagcttatg gtaaaaaaaa aaaaaaaaaa aaaaaaaaaa 39420

aacaaaaaaa aaaaacaagc aaacaaaaaa aaaaaaaaca aagaagtaat gggcaagggc 39480

catgaaatag agaggtagct cagttggtag cattctcagc accacataaa acaagtatct 39540

aggtacaaac ctgtaatccc agcattcagg atctagagac aggtgagcca gaagtttaaa 39600

ttgatacttg attacatggt aaagtccaag ccagcttgga ctacatgaga ccctgcctaa 39660

aaacaataat caaacatgat ttaaaaaatg ctaaaatgtc agaatgaaac ctagtataaa 39720

tcaatagaaa acaaaaggaa aaaaagaccc agaagaccaa aacgatcttt ttgtgtttat 39780

aggcgtaagt gcacagacgc actgggtcat gatttcagga caactgctgc tgtcagttct 39840

tactgtctac ttcctggaag gcagggttac ttcattgttt gccactaaat acagcagaca 39900

agctggcctc agagcttctg gggattcttc tgtgtccagc ctcatgctgt aggaacactg 39960

gtattttaga cgtatgctat tgtgcccagc tttacgtagg ttctcttggc cttgggctta 40020

cgcgcaagct ctatgcctat acccactgag ccggcttctc atccggaaat atgcaagtat 40080

cacaaatact gatgtgctat ggttagggaa ttgtcaaaaa aatttaaaaa gtcatcatgc 40140

cttaatgcat ttcagaacta ggagcctaaa agaagtgtga ggccatttgt gatttgacta 40200

ctttcttttg actttctttt ttaagattaa tttattttta tttatatgag tacactgtag 40260

ctgtcttcag agacaccaga agagtgcatt ggatcccact acagatggtt gtgagccacc 40320

atgtggttgc tgggaatcga actcaagacc tctggaagag cagtcagtgc tcttaactgc 40380

tgagccatct ctccagacct cttttgactt gtacaaagca ttgaatgaaa atgtaatgta 40440

gggccagaca tggaggagtc ctcaccaggc agagccaggc agatctctga gttcaaggcc 40500

agcctggtct acagagttag ttccaggaca gctagagtta aacagaaaaa aaaaatctgt 40560

ctcaaaaaca aaacaaaacg acaacagaca aaccaaaaac caaacagaca aacagccccc 40620

cctaaaaaaa tatagagaga gaacatagaa agaaatgatg tatagattct taagctatta 40680

aaacaactgc tttaccaaga ttacacgaaa atttgcttca aggagaaaag ttctgaaaga 40740

gaaagaaaat tgactgtatg atgagtgttt ggctatgttt acattaacac aaagttcttg 40800

ctaagctttg tggcagtctt ctaaaatctg tgctcagctg aagaaaaagg tcagaaggaa 40860

gcacagcact gggcatcagg cttctcaaga agaaacctct caatgggaga ggaggcacct 40920

agcctcaccg agacttgaag tgccaggatg ggggagtatc taggggcccc ccacctgctc 40980

agagaagaag gggaggaggg ttggggatgg gggctgggat gggggcagtg agcaggatgt 41040

aaagtgaata aataaaacat taaattaaat ttaaaaagag agagacttca gtagaatctt 41100

ggaggttgac agtgatgtag taggaatgga ggaaagggaa agcagaaaga gagactgaga 41160

acaggagatg tggagagaag tcttaatcca gtgtggtgct gtggaagtta acagggtacg 41220

gtcggaagaa aagctcaaat caaaaggctc tttcaaagct gggccagggt gacagatacc 41280

tgcaatctca gcaagaggga agttaaggag ggaagattac cacttccaga ccggagaggg 41340

ctgtacacct tgtctcaaaa atacagaagt caaagttgat gcaggaagtt ggtgtaagta 41400

gtgggactcc tgaaatagca agcaggtggt gcacaatgta ggtacaagca gttatagctt 41460

gctgaatttg gggatgggac ataatcgact ggttgtgtat gctagttatg gctcattttt 41520

aatttgaaag aaaatctgaa tggactgtgt ccaaggcaca cagtagtgaa tattcagtaa 41580

gttgttgaac tttcatcaca gagtggttgg gaatattgac tgtttcagat aggtctgtgg 41640

tttgagactt ggttcatcta tttctagcta tgtgagtttg aagtgatttc tctaaagcca 41700

gttgctctgt taagtgttaa tatcacactc acacacacac acatgaaata atttttaatt 41760

tcaataataa tatttctaag actctagttt tctaagaagt agcttccatt ttacctgtgg 41820

agcaagtagc ttaccccgca gtgaatatgt cctctgtgat tacgagaggc taaaatagaa 41880

acacagaaag acgtgctgat acttcacaga agttgttatt acctatactt caggcgctgt 41940

gatacaggtt aggtggatgc caatgagacg tgatactgat caatgagata ggagtattaa 42000

atgctatttt gggataacta tattttctat aaataaatgt taattgggca tggtggtgca 42060

tgttggtaat taaagcacct tggagacaga gataggaaga tagcaaattc aaagctagcc 42120

tgggttatat agcaagatcc tatggagaga ctactgaaac ttctagaatt gatcaaatgt 42180

gagaccataa agggacatga tgaaagaaaa tctcaaagtt tgtaagaaag aatccaagga 42240

gtttaatgac ggactcaccc aaagggaaca aattttgtgg gcaacaagtg tctcaaacgt 42300

atttgcctag agactatgaa aaatggagac gttggatatg aagcctggta tataaggaac 42360

tagagttggt gagggaaagg gacgaggcca ggaatgggac tcactgaaga gagcatctgc 42420

ctagcatgct ccaggtccgg gctttgagtt atagtactga gaaaaggagg ggaggagatg 42480

gcagtgaggt tgacttgaat aactcttgtt gaacttcacc tccttttttc aggtctcagc 42540

tagactctga ccttctgtaa tcatgcctgt cagtaaccct aggcaaaata aaagcccaag 42600

ccttcctgta ttactgcagc tccagatagc accttctctt tctgactcct aagcctagca 42660

tatatatata ggtctccagt ctttaagaca aaggtcttac cacatccatg gaaatggtcg 42720

ccgacaccta ccattcaaca tgtatgacca tgatagccct ggtatgatgt gctgtttccc 42780

cccactggac aataaactct tctagagaaa agaggctttg gggtcctaat ggtttatatt 42840

cgtggcactg gactcactgc ctaatatgta ttaagccttc agtgaaggct tggtgagtgt 42900

cgatgagtca tgagtgaatg tgttagacac tcaggttgaa atctgccaaa ggcattagga 42960

aataatggca ctgaagtttg agtaatcaca ggaagagatg gtgagcctta gcccagagtg 43020

gcacacaccc atccagccta gagactggca ctgttgactt acctaatttg aggaggaggc 43080

agggtggcag tttctgttgg tacaggtagg aatgtgaagt gtgggtgttc caaggcgctg 43140

catgctttga atggaaaaag gtgacaaata atatctcctt tgttgttgtt gttgttgttg 43200

ttgtttttgc ctgctctaga attgcttgcc cacaatggtg gctacatctt tgtgatttcc 43260

tcctcttttc taacaaaata atacattctt ccatgttttc atctcctctt gattgctttc 43320

tgcttaggga attttaagtc atagacccgg caatctttaa tcaacatttt tccaattccg 43380

tttttaccac atttactagc ggctgtcaat gaagtctatg gtgaatatcc tgtaggttac 43440

tagggtagtt gatcagcttc cttgctttct gctgggctgg aaatgaaact cttggcacta 43500

gacaggctcg ttaacgccga gctacaagca cagctgggtc cctgttctat tctttatttg 43560

cccagttcag ttccaggagg ggggaaaaaa gtacagaatt cctaatccaa actgattacc 43620

catgaaggag ttcctgaatg tgccaccctt cctcctgatg ctgccatggt acagaatgac 43680

acgcactagt aaatccactg ttcaataaac tatattccaa atgtgagtac ctgaagaaac 43740

cggatgagga ctccctccac tccccccccc ccaaacctcg aacagccttc aatttttgag 43800

taatagtttc aggtggtttt aagtaaacca ttctcaaaaa ggatgtgtgt ccaaggtttc 43860

tgtatagtta catgagtgga gtggtttcat aactgctgta ttgttagtca aacactggaa 43920

ttagggatga cttcataagc ataagattgt tctatgggtg acgccctgaa tctgtaccct 43980

gttgtacacc gtatcctttt atgagaaaaa caaacaaaca aggtttgttt tatacccaga 44040

gttgaaaatc tgttttccta tctctcagat gtttcagaaa gagagctccc ccaactcctg 44100

ctgctttctg tgttcagaac tgtcaggcag acaaatgaga acaaaggcag agtgaaaagt 44160

tttgcagact ggagagcctg ggagcattat tccttggcca ctatttcatt tgcctaaatg 44220

aaatggaggg aaacatatat agttctgctc actgcgtatt agtactctta cataaacaaa 44280

gttcagtttt gtggtaggac aggctgcagg gggaggggta aaaaaaacat ttcaaagggc 44340

ctttcccttg tctgtcattc attattaaat tggctttaaa cgtgaatcgt aaccttactt 44400

ctttatcttt tattcagcat tattgctcat gagaaaatgt tcagatggag aggaatgtga 44460

caatatcaac aagctgatga cttcgaagta ttgatactaa aattactttt attttttcaa 44520

attaatcact tgcctacacc tcaagatctt tggccttgtg aaagagccaa attgagtcat 44580

atgtaaaaag tgactgacag gttttttttt ctaatgcttt cttcaactgc gttgactgaa 44640

ggcttgtaat ttttatatat gagggaggct tgttatactc tgtagtcaat aattataggg 44700

gagatgagac agctcttcag gtatcccatt taaaagactc tcagtagaac ttatgcctga 44760

tatttattgt ctctcacaat tacttatgga gttatttatt ccccgttttc tttctccgag 44820

gatttgaagt tgctatataa tggtttgtag cttataattt gccttcacat acgtcatctt 44880

taatctttac gaggactgag ggaaacaggc attattagtg ctatttgcaa atgagaagcc 44940

gaggctcaga gagatgaaat aaacatcctc acggactgac agttaggaag tagctagacc 45000

agggtactga ttgatgatgg cctctcattg catgcccaag aggaatccac tatgtccgtg 45060

ggagaaaagc aattgccagg catggcaagc attcaggaag tatccagagg cagcgatgat 45120

aacaaattgt ctgtttcaca tgagagcccg tctaggatac tttgggagaa catgtatgaa 45180

aataaagaaa aaagaaaaga acagaaattg agataatttt ggtaagatct aagcatttcc 45240

taatgctata gaaagaccac atagttctat tcccagcacc catagagtag ggtgggcagg 45300

ggcaaggggt gcaggtcaca accgtttata cctaaagatg cagtggactg atgttctctt 45360

ctggcctcta caggcactgc actcaaaaca tgtacaccca acacacatgc acgtgtgcac 45420

atcgcgtgca cacacacaca cacacacaca cacacacaca cacacactca aaaagaataa 45480

taaaatattt ttaaagttag gggtgaggat aaagggggag ccaaaaggcc cgttgaggag 45540

ggatttgaaa gggaggaagt ttaacggaag ggtgcacatt cctgtcacct gcaataaggg 45600

tgacaagcag gggtggttta cattgtggct taaatctttt cattttctta ctgattaaaa 45660

aaaaacagaa gtgatatgta gtatgtgaat gaagtaagtt gtatagtttt atttttgctt 45720

gacctaccct aagttattat ggattcaaac taaccaaact agaaagggcc tttagaaata 45780

gaggtcaaac caaatgtttt agattttctc actccaatgg cttttgtgcc gctgttcact 45840

atatataatt cgtggaaatg gctaattttt cattaaaaac atgatgactg ggcatggtgg 45900

cataactctt tcattgcagc ttgatgaatg gtagaggtgg gaggttgcca gtagtttgag 45960

gtcaggctgg agctacatag ggagtgcctg tccagccagg actacaaaat cacaccccaa 46020

tttaaagcaa accaaaatga tcacttaaaa tgtatgtaaa cagaagtata attaatccta 46080

tttagtattt ttgtgattgg cactgttaaa catcgtacga tacgtaaaac aaacttggaa 46140

ttatcaccac agggttaaag cttaatgttg taggatgttt gaccatatta tgaacccaga 46200

gattctggta tttactaaaa tagcctgttt ctagttgtgg tgtaactcag ccttagcacg 46260

catctttaat cgcagcactt gggaggcaca tgcctttaat taaaaacagt aaaggtaaag 46320

ttaatgtgta gaaagaagca cccgtgtttg taagtcatgt ctaattgagt ggcagatgac 46380

taataagatt tgacagaata ggatatgctc caactttcag gagaagagag aggaaaggga 46440

agctactacc taaggggcaa tgcagagaga aggaggcagg catttttgct gggagagttt 46500

tacagaagac cgaaggagcc agaagattag aacatactgt caacgtgagt atggggcaag 46560

cagagcgatt tgggagaagg gaagagaagc cagattgaat ccgttagtgt ggagaggggt 46620

ttgaaccaga acagctaaat tgaattagct gacgagagtt cagaaaggga gagaaagggt 46680

gagcttattt agcaatgtct cagaggctaa aaacattcta ggcctagata aaattataca 46740

ttggacgtag gtgagatgct tctaggacta ggcctaggtt agaagactga gggagtaggc 46800

tacagacaca ataattactt caaagcaaat aaaacttaac agcttcagct acatcttaat 46860

tttttttaaa aatcatttta ttatttttaa accaagtggc tacttgattt tgagtcaaga 46920

ttgtttcttt gcctgtttgt gtggttattt gttgcagata gggtgtcacc aaataccctt 46980

attggcctag gacttgctat gtgaaaccaa atggccctga actcagagat cagcctgcct 47040

ttgcctccca ggtgctgaga ttgaaggcat gggccaccac tcctgactcc gcattttttc 47100

acaatgtatt ttagtttggg aaattaaaat ataatgacat aattttcccc tttcctcctt 47160

acaacttcac gcatgtaggc ctctctctca aatcatggcg tctcttttta ttggatattt 47220

tatttccaat tcaaatgtca tcctctttcc aggtttctcc tcctcaaacc cccatcccat 47280

tccccctccc tcatgacttc ttttttttgt ttggttctta tttaaaatag ctacctctgg 47340

gctctgggta gacctcagtt aatgctatgc ttacttagta ttcctggaac cataggttct 47400

attcctagta ctgaatatat caggcaatgc atggctataa tcccagcatt taaaaaagga 47460

gaagaatgga tgaccgtgac ttggtgataa caagttcgag gccagcctgg gctacttaag 47520

accctcaaaa actggtgaaa atgtacaatg ataaaatgta tgtctaactg gagatacttg 47580

tttcttttaa aaacattttt aatatttgat atcgtaattt aattacattt ctcccttccc 47640

tttcctccct ccaatccttc ccatgttccc cacactctca aattcacaac ctcttctttt 47700

ttctcctctt ctttattatt gttgtgtata tacgtatata tttaaattat aaattttaaa 47760

tcatatctag tatataataa caatttaaca tagtatgtgt ttgttaacat atagttgtgg 47820

aatctatatc aaaaccaaat atatttgttt tgactggttt ctttctctcc cttttaataa 47880

gagtgtttga taggtctcag tatgattgac atttgaaatc agtatcttct tttacaggaa 47940

ttattagcca agtgatttgg actttcacta tggcgcagct ggaacacaca cacaaacaag 48000

atcctgtcat gcagtgcttt tgtgctatac cttggtgctt tcaaatccgt ttagtgtagc 48060

ttaagatttt atcattggga agttgccacc aagtgagacc ttctgagttc aacttcagga 48120

atccacatac tgaagagaga atctatgcca acagttgccc tctgacttct aaatgcatgc 48180

tgtgacagan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngag agagagagaa 48240

agagagagag agagagagag agagagagag agagagagag cgcttgtcgt tggttggttt 48300

tctttatttt gggtgtagat acatggttac ctattcagtg ttttcgtagt ttgcagactt 48360

acttactgtg tttgtaattt agcaaacgct gctgttacat attcttcatg aactgttttg 48420

aagaaagagc cttttgcttc cttcccttta gatcttcaca tggacgagat ggcaaccact 48480

cagatttcca aagatgagct tgatgaactc aaagaggcct ttgcaaaagt tggtgagtgc 48540

attctgccac agccagagga aaggcgcatt tattgggtct taaagtgaga ttgctagaaa 48600

ttaccataga tcagactttg cctgagcaat acagtgagac tgactctaaa tatcaaacca 48660

gaactagatg tgtaaagttt tagatattat agggaaaact gtattaattt gggcagacat 48720

tgcttacttg aattcaatcc tggggatcca cttggtggaa ggaggaaact acacacacac 48780

acacaaacac acaaatgaag acataaaagt cataaatttt attacgattt ctttacaagt 48840

actgtattta tatcatttgc agtctcccct ctttttcctt caatgtccat gtccccaata 48900

ctccctctca aattcacgaa cttatcttca tatatatata catatatata taatattata 48960

gatgtatata tgtatatata tgggtataat atataagtac tatatatgta tatatgtgta 49020

tgtatatgta tgtgtgtatg tatatatgta tatatagtat atatgtgtat aataatatat 49080

aagtattata tatgtatata tgcatgtgta tatgtatata tatatgtata tgtgtgtatg 49140

tatatatatg tatatttgta tatacctgta tatatgtgtg tgtgaatttt taaatttgaa 49200

agatatttta atagtatcta gtatttaatg tggtcaatca tttttatcct aaagtcctag 49260

tcattaaatc tagggtgatg tggcacatga aaatttggtg atatatgttg tataattaga 49320

atatttttca tatcatgttt ttctgaggtt agtgtttagg tgaatgctga agcattagtt 49380

agagttccct gttcctgtgg tatttatttg ttgagaaaag agtctcattc tatatccagt 49440

ctagtcttga cttcactatg tgtactgttc atcctgctaa caagcatcaa aaagggaaag 49500

gcatatcaga atgtcatgat tttcccctag aacagagtat tgaacccaaa atctcatgac 49560

aagcaagtcc tctacagttg agcggcaccc tcagccattt atgttttgag acagtctccc 49620

tgagctgttc tggctgatac caacttgtga tcttgtcacc taacacagga attggaatta 49680

tagacacatg ccaacataca tggcttgttt tctttgttgt atatttgttt gtatacatac 49740

atgctagacc aactatctac caataagtaa cattaccatc ttttcctttc actttttatt 49800

ttgagacaag gtctgcccag attgcccaga ctgaccttga aatgactcta tagcccatcg 49860

aggtcttgaa tctactatct gtcctatttc ctggccttcc cccagtagct ggcaatgcaa 49920

gcctggacca ccgggcccag ttttgttttg ttttgaaact tttaacatca tatcacacgc 49980

cttccaattc gtagctatgg aagcaatgca tattgttttc attctatgca aagatcaagt 50040

ctgtaaagga taaagtgaaa tggagtaaga actaactgct gatatgaata ctcataagac 50100

agagcatgtg cttaagcttt ggctctcaac ctgtgcttat aaacacagac ccctatctcc 50160

aaaaatatct acattacaat tcattacagt agcaaagtta caataatgaa atagcaacga 50220

aaacaatttg atagttggtg gtcacccaat atgaggaact gcattaaaag catttctcta 50280

ttagaaaggt tgaaaacccc tggcttaagg taaacccaaa ctacagggag taaagttaga 50340

gtataatggt ttattattaa atcagaacta ctctgaaact gatgttaaaa ttcctattaa 50400

tataaaattt aaaaagatac ctatctatgt tctcttttga ttcattcatt cagtcattca 50460

gtcagtcagt cagtcactct gtcatttttg ggacagtctc ttttacacca ggttggtctt 50520

cagattgact ccaaatagcc caagatgact ttgaactctt cttccccttt gtccacctcc 50580

atagggctaa gattaaagac attagcacct gtcttagggt tttattccta tgaagaaaca 50640

ccatgacctc aacaactctt actaaaaaaa caaacagaca ttgagttggc ggcggcttat 50700

agtccaaagg tttagtccat tactgttatg acagggtgga tggcatgcag acagacattg 50760

tgctggagat gcagttgtga gttctacatc ctgatctaca ggcaccagaa ggagactgtg 50820

tatcatactg agcataccct gagcatctaa taccttgaag cctgccctcc aacaaggcca 50880

cacctacttc aacagggcca tataccctta gtgtcactgc ctatggtcaa aaatactaca 50940

catgagtcta gggggggcat tcttattcaa ggaccaagcc ttcgtgcgtg ctaggcaaat 51000

gcttcgccaa ctgagctaca tacctacgta tgaacctaat ttctataaca gcatttatat 51060

taatagaaac ctcataccct gaagagaata ataatgcccc agctagagtg gctattcata 51120

tcccatcatg tctagataga aaaggttaaa ttgttctgaa tcatcatttt atgtgtgtat 51180

attttgcaaa gcagtataat aacttgctgc acatatgact cactgggaga agggaagtac 51240

ataggtgaaa tagtcaattg atgaaaaaga gaaaatgagt ctttggtcat aagcggtggt 51300

gttatttttt cccttttgtc cctttagaac tgttctttcc ataattgttt tagaagcgtg 51360

ttttctctca cccttgtctt ttgtttccat tcatctgtca gtggtaggaa cagtggagtt 51420

agtttctgtg cagggaccat gacttactaa tgctgcattg cttcttcacg atgcagggcc 51480

tcctgaagga aggccttgac ttcatgtatt gcctcttgga catatttatg ttcactgttt 51540

cttttaaatt aacgttgaat gatatgtgac ttactaaatg gaacttggag tactttttat 51600

gacttaacag cataactgct ttgctaagac ataaggatac agaattgacc aattattgcc 51660

gtagcgagaa cagggttaaa ttatacttaa caagggaagg atcaattaga acagtggttc 51720

ttattcttcc caatgcttga ctctgtaata catttcctca tggcctagtg acaacctcaa 51780

ccataaaatc atttcattgc tacttcataa atagaatttt gctaccctta tgagttgtaa 51840

tataaataga tgatatgcag gatatctgat atgcaccccc tgtgaaaggg tcagataccc 51900

caaagaggtc accacccaca ggttgagaac cattaggtta cagtttttac tgaaaaaatt 51960

cagaacatca acaaaaacaa aaccgcatca ctgtgtgagt ctttgcattg cataggcagg 52020

agataggacc gtaagagcta ctgaaaccct tcccagacac aggtttcact atacaacccc 52080

taaataaaca acctgtccct caaaaatagg gaggctggta tttctgccgg ttcttattaa 52140

tactctttca tttgtatttc tcctctaaat acctagtgca gatggcccac gtgttaggtt 52200

ctcgatgaat atttaattac ttgaatatca aggaaacaca gtctttgatg tactgagatt 52260

ttaccatttt caattgtaca ctcaaagggt ctttgctaca aagaatttat tattaaagtt 52320

ttttggccat tccactgaag cacataaaag caggggtggg gtgtctgtgt gggtgaatgt 52380

gtatgtgtct gtgggggtgg gatactatgt gtgggaaatg catgtgcaga ctctaccact 52440

cagaaactca ctgaaaagct cacccgactc cttggatttt cctacaaacc ttcgtagttt 52500

ttacatggca ttggatacga agccctcagg caggtgtcca aggtgttatg gaactatttt 52560

ttttaattta agtgtccctt atttattaaa caaagctcta gaacgttatt tccctggcga 52620

tgatgattct tccctactct gtgtggtaag ggagagcaga gagagctact tggtctcact 52680

tcatttgtga caaaatatgg aaatcatttt cctcatctat taacagtaat agccttgatt 52740

atctacttag aaattcagca aaatcacagt gggcagagaa aatctctgta atcacgacta 52800

atcacagggt ttgttcagtc aattaatagt gctttgaaac tctctttacc agcaataaat 52860

tgagtattag gatttaaagt tctttcacta gctccttgca cctgcttatg gttcagctct 52920

aatcatatgc aattatttta attataccaa caattgtctc agggttagtg gaaatcttag 52980

tgccaccctt cccccaacac aaaatgtttc tgttttaatt taaatactga catcctgcat 53040

gattctcttc taaagagaat gcagcttagt atgttggggc ctgccaagga ttcttgaagt 53100

ctataatcct tgcactaggg ttgctgaatg aagaagatct caacagaaca gggctagcca 53160

aggctgcata gggaaaccct gactctatat ttttttaaga cagaaatggt gacacgtact 53220

tctaatccca ctacttgggc acatctttga cagttcaagg ccaaccctga gttaaaaaaa 53280

aaataaaaaa aaataccaaa aaactaggag agagcgggtg ggttcggagg cgatatagtt 53340

ttcctttttt gggtggtggc agtacatggt ttaagcctag tactggaaag tcagaagcag 53400

gtaaatctct gtttgaggct atccttgtat acctaaggag tctcagaaca gccagaggta 53460

cacacagaga aacccagtct tgaaagccca atatgggtgt atgtaatttt gttttcattt 53520

tatttattca tttacaaaga gaatgtttac cagatacaag atgtcttttc ccccccccta 53580

ggcaagcgct ctaccactga gctaaatccc caaccccgca agatgtcttt tttattctaa 53640

gactttaatt ttgtattgtt acttccctcc attaatgaat tttgggtgac ttaaaatact 53700

ctcagtttga cattggtcct gcaaccactt ccttaaaaat aggacttcgg agagcaggaa 53760

agctgtctca gtgtttatgg ctactcttgc aatagaactt ggttcaattc taagaactca 53820

catagggggc ttaaaacctc tacaactcca agtccagatc aaacacgctc ttctgaactc 53880

tgtggacagc tgcaatcaca tgtatgcatt cccccacaac aggtacacac ataattataa 53940

ccagggcata tctataaaga atagtcataa aatatgactt tttcttcttc catgaagcat 54000

attctagtaa acttttgtaa acatgaaagt caggattttt aaaacacaag aatactgggg 54060

ttacttaaat gaccaggaat agtatgggtg cattctttga ggttcccacc aaaagaagcc 54120

aaaacacttt ggttatggtg attttaactg taccagattt ctcttagtcc agaacatgga 54180

actgtttcca attacttgat attctgtagt atagaaagca catcacaatg ccacaaaaaa 54240

atggcagttt tgtccagttt cctttcagtg ttgaaagaaa ctgcaccatt gcagatttgt 54300

ttaattctac ctaaaaatag aaaaatataa aaactagcag gctagttttg tcaggaagtt 54360

gtggaggggt tggttagtat atagaagcaa ctgggcattc accaaacaca cacacacaca 54420

cacacacaca cacacacaca cagagagaga gagacagaga gagagagaca gagagagaga 54480

gagagagaga gagagagaga gagagagaca gagattgatt ataaacattt ctgaatagcc 54540

agtcctgacc ttctacacca cttttggggg tccactcctg aatgtccaat atagagtact 54600

aattagcaag ttctagaata tccttaaatg gaactcatta aatatccacc tcatgttcgt 54660

tgaactaggt tacctcagga agtagatact gagccacagg gttagataca catctgaaga 54720

taattagtag cagccattcc ttgttttttt tttttcaaga tggggattct ctgcgtagtc 54780

atgaccatcc tggaacttgc tctgtagatc agaacagccc agaactcaga gattggcctg 54840

cctctgcctc ctaagtgcta ggattgaaag tgcccacaag caatgtccag ctaagttttc 54900

acactttaat agctaatgaa agctcggctt tactacttag atcttagggg gtaataaata 54960

aaatacatgt atgtaggctc ctgtaaaagt cttttgacca gtggtgttag aaaaattatt 55020

gttagatttg ttaattttat tttctgtgtt ttccctgcat gtgtgtctgt gtgccacatg 55080

tgtgtctggt accttcagag gtcagaaagg ggtgtcagat tccttggaac aaaagtcctg 55140

aatgatttcg acctcccagt ggggtgctgg gaggtaaaac tgagttctct gcaagagcat 55200

ccagtactct tagatactga accatctctc cagcctgtgg gcaagccttt aaatcaatga 55260

ttttatatgt actcaacatc gtgcatgttt ctataagacc attgttatgc acttaacttc 55320

tctatcaatt tcttttattt aaaaagtttt gagaggggtt ggggatttag ctcagtggta 55380

gagcgcttgc ctaggaagcg caaggccctg ggttcggtcc ccaactccga aaaaaaagaa 55440

ccgaaaaaaa aaataaaaat aaaaagtttt gagaatatat tagttaaggt aacctagact 55500

ttttcaattc cagccttttt aaaaaaaact gtatatctct tgtggattca ttgtcttctt 55560

taaattgtaa tgagcactgt ccacaatggg ttaatgtaga tgccttatat gaaggcacac 55620

agttgcaact tcttttctag aaaaacacca tatttagaca gtctcaagaa gagtaaaaga 55680

cacacacaca cacacacaca cacacacaca cacacacaca cacacacaca ctccctgacc 55740

gtcaaaggaa atcctccttt tttcttactg ttagatttag aacacaaagt aacaccttaa 55800

aagatgcttt ggaaggcatg ggttacaatg cataccacct aggcaagggc agtgtaaaac 55860

agcggccaac ataaaaacca cttgaaattc tggctcagcc ttctccagac aaggacttaa 55920

aatagcttaa ataacaggat gcaaggggag ggaaaacatt tccttttaag gatatgcatt 55980

cttggtgtga ttcaaacttc tgcaggaaca taagtttatt tactttgagg ttacttgggg 56040

gagggtgtgg aaggaggtaa ctggcattta aactaatact tggttaaaaa aaagagaata 56100

ataagatttc ctttaaaaca aagtgcagat gggactctac atgtgtcatc tgaagcacag 56160

agagacagat ggagagtgaa ttctttgtaa ccaacagttg gttcgtttct gatgaattca 56220

gcaactggac tgaaagatga ttctgtctag tacaatcttg agtgcctcct agtgattctt 56280

ttcaagtata atatttttca gtgtttgttt tttttttaaa taaatttggt gtatttgtta 56340

aatgttagct cattacttca tgcaagcaca tggtttggca gccagatagc attccttttt 56400

tttccgccta tggcatgtta ctctactcat aataatctta ttttcagaac tacaatgctt 56460

gactgttgtg gaaaacacaa gctgtgtgta aatcatttat tattttaatc agacgttaga 56520

gaatgggata gcagaagaga cgagtcttcc aaatacaaag cctcttcccc ctcctgccag 56580

tgcatagtta gaagcagtga atgagccaga aatgggttcc agaatgttgg cagctttaac 56640

gagcaaagaa agcgctacca ccgcgcctgc tgattgcact tttagtttcc agttaaaact 56700

gcttttgatc tgggcctgct tcttagatgc tcatatgatt aaactgttac gttcatatca 56760

aattcagaat ggaaaagttc accattaaac gtggttttag attttggtga atcgcgttac 56820

gggtaggatg gattctggtg aaaccagcat gtactccctc ctttttccaa caggtcaggt 56880

cactgtcact acttcaggaa tgtagtgctg tgtttcagtt ctgtcttttt aaaccggaga 56940

ctggaaataa aatcagcatg aaatgctttg aaaataatta cacactgttt tgtgctttgt 57000

aaggacttta ccagctggga ggttttgtaa aattaattgt tagctgatgc cattttctgg 57060

ctctctctga cttcttaatg acattgaaat tgttcccttc cagtccagtc ctaagaaagg 57120

cccgttgtga ttggctgata cgagcaccaa aatgccacaa tgtaaacagg attggcatct 57180

attgagaaaa tacagggagg gagccaagta gaactgatga aaggacggtg acacaatgat 57240

acacaatatc tccccccacc acctcatttt tatttttatg gcttggtttc tctgtgtagc 57300

ccctggctgt cctgcgtctc tctctgtaga tcaggctggc ctcacgggga tccaaatgcc 57360

tctgcctccc taatgttggc attaaagttg tgcaccacca ttccctggat agatgatata 57420

cagtctaaac atgaaaatta tgaaggataa atgtcctcag gaattccaaa gttgtacaaa 57480

aattcgcaag ccattggttc aagtgagaac caagggttct gactgattat tgtcttattt 57540

gggttcagct tagtgtcttt gacagatggc tatggtcatt tagaaaacca aattacagat 57600

cagatggttg ctgtgtgggg acaggattag aacaacagga gtgagattaa atgttatctg 57660

agagatattt ccatcccgtt ttagtttcat gatgaactgg caattagtct ttttggggta 57720

cttgttctgt aatttttggt cttggctatt tagttctgtt ttgaaaaagc tcatctagga 57780

ttagagcatt cttcctccag ttagtgtcca agaattagtc tgtacagtat tttagagcat 57840

ttgactctag agcttggggt ggagtaatgg agcttgtatt tagcatgcat aaagccctgg 57900

ttaaattctt accaaagcaa agaccagaaa gaatgtaaag atatggggct ggggatttag 57960

ctcagtggta gagcgcttac ctaggaagcg caaggccctg ggttcggtcc ccagctctga 58020

aaaaaagaac caaaaaaaaa aaaaaaaaga atgtaaagat ataccatgta atgccaggat 58080

cctgatcctg tcacacacac acacacacac acacacacac acacacacct tcatataact 58140

ggtaaattgg tcagagataa ttcataattt tgcagggcgt ggtgccatgt gcctataatc 58200

tcagcatttg gggaggcaga agcacaagga tctctgtagg ttacaaggcc agccagaact 58260

ataagagtaa gaccctgtct caagctcaaa ctgccaaata aaagtgcaga cacaacacag 58320

ttaatatttt agaggttgtc ccaaagttaa ctgctgtaag tcacacttag ttcatgtcat 58380

aaaaacatcc aaagtctgta ctgcaaccca gaagaatgtg caggagccat gaaaaatggc 58440

ctctttcccc tttgttcact gtgctgaagg atgctagacc tctgctgttc caggagcaca 58500

gggcatgcac atccgcatca catatggtga gagctctttc ccctacttga aatcgctcaa 58560

tcctggtgtc tgcctttgtc tcacacaagt ctatattcaa atggaagctc tcgcacaatg 58620

gccgtccttg actattctgt ctgcataatg gcggtccttg actattctgt ttgaacagta 58680

gcccccacaa tgtcattata acccagagtt actcaacctc agggccgctt atatgttgag 58740

ctgcaccagt ctgtgctgcc tagtggccaa cggtgtgtca tatgtgatgt gttgctggag 58800

attctgtggg gtccttgctc tcgacccact ggatgccaag gagcatcttc tccccattca 58860

gatatggcaa tcagagacat cctcagctag agctggttgt tgggtgcacg gctgtcatcc 58920

caacacatac aaggctgaga cagatatcag cacaagtcac tgcgagttct agagtaaccc 58980

aggcagcata gtgctactca gtctcaggaa cacatgcaga atcatgcaga tgctcctcaa 59040

ttccctgcct gggtgcagag gaatggctgc tgctccaacc ttcttctcca cttcagtttt 59100

catcgaaggt ccatttgcat tgtcatatgg tgcattgtca tatggtgcac tgtcatatgg 59160

tgcattgtcc tatggtgcac tgtcctatgg tgcattgtca tatagtgcat tgtcctatgg 59220

tgcattgtca tatggtgcac tgtcatatgg tgcattatcc tatggtgcac tgtcatatgg 59280

tgcattatcc tatggtgcac tgtcctatgg tgcattgtcc tatggtgcat tgtcctatgg 59340

tgcactgtca tatggtgcat tatcctatgg tgcactctcc tatggtgcac tgtcctatgg 59400

tgcactgtcc tatggtgcat tgtcatatgg tgcattgtca tatggtgctg tgcctcaaag 59460

tcgcacgggc tcagtttgca ctgaggttag aatgtgggat ccgtaaggcc agacattttc 59520

gggtactttg ggttctaaga agagttcccg gtgaggcttt tggttaatcc tgttcatttt 59580

gagacagagt ctctctatgt agccctggct gtcttaggga ccacttttta gaccaagctg 59640

gccttgaact cacagaaacc cgcctgcctc tgcctccagg gtgggggact aaaggtctgc 59700

accacctatc tgctttggca gaacttttaa gtaaattccc tgatcaaaac atttccaaca 59760

tggtcatcat gggtcatttg ttcatttcta gattattttg tcctttacat tttaacatac 59820

ctgtacttca atacactaaa cataaagaga tcatttatag aatcactttt tttttgtttt 59880

gtcttttttt tttccggagc tgaagactga acccaggacc ttgagcttgc taggcaagtg 59940

ctctaccact gagctaaatc cccaacccta tagaatcact tttcgtgcct gctttacaca 60000

gtgctccctt attctgttga actatctatt tgtctgagat taaagtttgt tcaccttaac 60060

tcaaaaaatt gaccctcgaa gctgttactc ctgctcagac atgctttgag ataaaatcac 60120

tgcgaaacca taaaacccat tttgcaaaaa gaaaagaata cagatcaaat cctacatcct 60180

gcatagagac agtagaaatt aggttacacc gtgacagttt gttgttgtat aaagtattgt 60240

ttctaagact ctccttgttt ctatgattta ataataactt agaaattata gtttcttata 60300

tttagggatg ttgtttttaa ataaagattt gtagacattt ttaaaagtat cagatacatg 60360

atataaataa aaccttaccc tttcaggaaa aatatacatt cctcttccac acatccttag 60420

atttcaaaga gtaaaaaata atactgtaat taggcaggcc tgtaaagtga tgatattctt 60480

acaagtggat ttctaatctt ttctgtttta tatttttgtt tgttttttgg tggttttctg 60540

ttcataattt tatgaagatc tcaacagcaa tggattcatt tgcgactatg aacttcatga 60600

gcttttcaag gaagcgaata tgccattgcc cggatataaa gtgagagaaa ttattcaaaa 60660

actcatgctg gatggcgaca gaaataaaga tgggaagata agctttaatg aatttgttta 60720

tgtaagtatg cgaaaagtct gattgtttaa agtcattgtt gttccactat atactggcaa 60780

tcttaaaata ctgttgcatt gaatggttaa caatatgtta atgctctggt accttgtaac 60840

atttttgtca ttctgactat aaaatttaaa tgcttcagct attagaatac ttaaagatgt 60900

caaaaaaggt acattgaaaa aacaaggtat agcacaatgt tcacagtatg cagaaacaat 60960

ctcctttggg gcatctttag agggtacttc tcaagatact caataacagc tttgaggcac 61020

tctttctttt tttctgtata agattgggct acatgagttt actgatacca tatgactcag 61080

ttttagaagt ttcctgagct gaacttttga atgttttctc acccccatac actatactgt 61140

tatttaactg tcccttttcc cccagggcga acgtattgat gattaaaagg tagaaggata 61200

ttttttcagt ttgtctggag aggcatttac aactttgttt cttgtctagt gtcatcaatt 61260

attcctcaag gttcatggct gatttcataa tacttctccc ctataagcgt ttagttcggt 61320

tcctatagca tggctgacag acatgtggtt ctttatcaag atataactat tttagctgcc 61380

agaattatag ccaaggtttt tgtgaattat agatgttcag tctagatttg ttaaattatt 61440

gcatgatgac gttttaaata gctgttacta tttctttcag ttaagaacac ccttaagggg 61500

ttgcaaagag agcttaacat ttaagagtac tagctgctct tccagagtac cttggttcaa 61560

ttgaaatgga ggtttagaac tgtgattcca gtggcacagg atatggcacc cacttctgga 61620

atccctaggc actgcacata agtaggacac atcctgtaaa ccagaacact ggtggcttat 61680

gatttgaatc ccaggattta ggagaaagag gtaagcagag ctctgtgagt tcaaggccag 61740

cctgttctac agagctaatt gtacaacagc aaaagccaga cacggtagcc ctgtctcaca 61800

ggggagaaag aataattgga aaaagcaccc taaaggcaga catggtggta catacctttc 61860

atcaagtcag aggcattcac tgaactcttg tgattgagtt caaggccaga ttggtctaca 61920

gagcaagttc caggccaccc actgctgcaa agagagaccc tgcctcggat aagatacata 61980

catgtttggg gttttgtttt tgcttgtgtt agggtttgtt tagtttagtt tgttttttga 62040

gacagtctaa ctttaaaatc atgatcatct tgctttgacc tcctgagagc cgagggagaa 62100

aggcgtgtac tgccttactt agcctacttt aagatcttgt gggtgttact atttattgat 62160

tgccttggtt tttaagacta cctttggccg tgtagctccg actggcctcc tgtttatgat 62220

gtctttctgc ctcagcatcc cgagtgctag gattgcagga gcatactaca ctgcctgact 62280

attcgtgatt attttaagcc tgaatttcag tagatcttgt taggccttgc tttcagtata 62340

ctgcaaacta ggaatcagtg tttcatattt gtcaccaact tcccagcatg cctgtcttcc 62400

tttttcatac ttcatcttgg caccaacata tctgctgtta tagtgaatgc tctcatcagt 62460

taccatgact caatatgaca actgaaattc agatatgttc cctctgagca gagctaacgt 62520

gaagtacaca ccatatggtt aggaaacaaa tagactacat agggctggag agatggcaca 62580

gtggtggaga gcatagattg ctcttccaga ggacccaagt tcaagtacca ccacccacat 62640

ggcagctcgc aactgtctgt aactccagga tacaaccctc atgcagacat acatgcaggt 62700

gaaacaccaa atacacacac acacacacac acacacacac acacacacac atatatatat 62760

atatatatga tttcttaaaa ccaaatagac ttgtcagata tggggggggg tggtcagtac 62820

taaaaactac agatgtgtac tattatactt acacagttct tgctattttg ggtgataaac 62880

tgatttcctc tcatcatttc tgtctccagt tatggctaaa gcgtttgctt tgaattctgt 62940

gttcgatagt aagttacaag tgactcgtga tatcagcact tgcatgtctc tgtgtgtact 63000

ttgcatgtgt cactgtgaat ttaaagcagg ccggagcggt tgggaaccat ggccagttgc 63060

ttttcctata acttacagcc gggagtcttt aaccaccaga aagaggcagc caaagtcatt 63120

agaatctgta gtctaactag tactgttgag gtcaaaatta tatataaaca tgtctacaac 63180

gaacaatcat gataaaacat tcaattttgt atattttaaa cactgcagtt tccacacatc 63240

atttagtggc tttcaagtca caaagtaaca ttgacttagt atgtacttta aaagtagagg 63300

cataataatt taggggaaag cagggatttt ctagagtaaa agaggaagga ggggctgaag 63360

agtgggcaca gtggtcccag tacacatatg gtggcccaga accatctgat gggatctgat 63420

accctcttct gttgtgcaag gcatacagga atacagcact catatacatc aataaataaa 63480

aaccacaaga gaaaaatcta ctttttaaaa attgaggagg acatctaaga tcaaagtaat 63540

atttagaagg atttccactc tctcttgttt gaggagaatc cgtctctgta gccctgtctg 63600

tcctggaact atgttgacca ggctgtcctc aaactaacag aagtggacct acctctgcct 63660

cgcctgctgc aattaaaaag gtgacctcga tgccttgctt caacatgaaa caagcatgta 63720

tgaaagagaa attgagttgg ccagcattgt ctagagggtg agaagtcttt tctagtcggt 63780

cctctgctca tcaagacact tggactggga cctggagaaa tggctcagca gcactaacta 63840

ctcctccaga aatcccagac ttcattccca gctcccaaac aaccaggaaa cccattgccc 63900

tcttctggtc tccaaggaca tcccaatgat gttaaagtga cccatatata ccatcatatt 63960

actatggaca tgactgttag aaaaaggggg gacattgaaa tggctgtcca ctctttatgt 64020

gctttgctgc tcatttaaca ctttgcaagg tgtcaaagaa aattgaacca aagcttaagt 64080

atacaagaac cagagactgc aactcttttt ttttttcttt ttttattaac ttgagtattt 64140

cttataacag tttcgattta ttcccttccc ggttccggcc aacatccccc tcccccctcc 64200

ttccttatgg gtgttccctc ccaaccctcc ccccccattg cctgccgctc ctccccatag 64260

actagttact tgggggttca gtccttagca gggaaccgca gggcttcccc ttccactggt 64320

ggttcttact agatattcat tgctacctag tggggtcaga gtccagtcaa cccatgtata 64380

atcttatagt ggacttagtc cctggaagat cctggttgct ggcattgttg actttggggt 64440

ctcggccccc ttcatcgaat ctcagttctt ctctgattcc ttcataggga cctattccag 64500

ttcagtgttt gcgctgcatt cggcctctgt attgtgctga ttctggctgt gtctctcaga 64560

ggcgatctac atccgctcct gcgtctgcac ttcttgcttc atccatctgt caattgggtg 64620

gctgaatgta tggccacatg gggtgcaggc tctgatgggt gtatccttca gttctgtttt 64680

gaatctttgc ctctccattc cactgccaag ggtattcttt tcctcattaa aaaggagtcg 64740

aagcatgctc agcattttga tcatccccgt ctgattcgtt gtctagggat ccccgtaggg 64800

taaatttcaa cgggcattgg gctttatatc cacccttatc aaattgaaag tgcataacat 64860

gtacttttct tgtattgggt ttagctcacg atcaggtaat ttttaaacag tttccaacgc 64920

atttgggcct taacgaaatt tcataaaccg tgtttttgat aagctgagta tattccattg 64980

tgtagatgta ccaacatttt ctgtaaacat tccttctgtt gaaaggcatc aaannnnnnn 65040

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 65100

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 65160

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 65220

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 65280

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 65340

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 65400

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 65460

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 65520

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 65580

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 65640

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 65700

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 65760

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 65820

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 65880

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 65940

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 66000

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 66060

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 66120

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 66180

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 66240

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 66300

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 66360

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 66420

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 66480

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 66540

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 66600

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 66660

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 66720

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 66780

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 66840

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 66900

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 66960

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 67020

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 67080

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 67140

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 67200

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 67260

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 67320

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 67380

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 67440

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 67500

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 67560

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 67620

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 67680

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 67740

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 67800

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 67860

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 67920

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 67980

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 68040

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 68100

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 68160

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 68220

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 68280

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 68340

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 68400

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 68460

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 68520

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 68580

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 68640

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 68700

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 68760

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgagactg 68820

caactcttgt tggctgttgg tatgagggaa gagaaaggac acatgttctt acatgctagg 68880

tttagtattt ttatgtaaat tagagtttaa aacagtattc agtgtaaact aagctacaaa 68940

gaataccagt cactattaat tttcaaggac agataaagga caaaagaggc tccagatatt 69000

gtaaacaaac ttcaaagcta ggactgtaat taataattgt ttttgttttg agtatcacga 69060

attgaagtta ctagtcttgt ttacttacat attttttagt ttattgaagc agatttcctc 69120

tgtgtaagaa ctcagaccct actggaactc tctctctctg tagaccaggc tggcctcaca 69180

ctcacagaga tctgcctgcc tctgcctcct atgtgctagg attaaaggca tctgctgtca 69240

tgctgtgcta ttgggctttt taaatgttga ttttttttcc tattttgact tattgtgttc 69300

aatgacttaa acacggtggc tgtgcacatg ctaggtaaca tgctgtattt gcacatgctc 69360

tactactgaa ccatatacac agaacactgg gttttatact tataaactaa cacagttgct 69420

ttctttgatt gtgaaagcct aatggggcaa tgtattttta aacagagatg attacatgtt 69480

gatttcttgt tttcacaatg atagacttgc tttcacatcg tggtgataag tctttaacct 69540

tttgctaata agatcggtcc acaggattaa tcatttaaaa atagcaaagt acttagaatg 69600

atgtccggca cagagtaaat gctccacagc tcttggtagt ttaaataatt atttctattt 69660

ttattgattg attcctttga gccagcgtct caccaagtta cctcaactgg acaaacttaa 69720

taattcaatg gattttcttg ctctcagcct ctgtggcaaa tgtgggactc ccaacccctt 69780

actagtactt ctccctctcc cctcccctct ctccctcccc ctctctctcc ccttccctct 69840

ctctctcccc ttccctctct ctccccctcc ccctctacct tcccccgctc tctgattttc 69900

tcctatttaa aataaatgtc gtcatgctct tttccaacgt taggccggca acaatttcca 69960

caagaaggaa aaattcaagc cagctatgtt ggaaacaagc cccatactag aagcaatcga 70020

tattttctgc aaattctttc tgaaatcttt ccttcttaag tcatatgcct ctgcattgct 70080

ctgggctggc tgcttggcct ttgcaggact cactttgggg tgaaaaagat cttaagtgtc 70140

tgctgaagat tttgagagaa agttggtgtg gctttggaaa ggatgccacc aaagaaacta 70200

aaaatgatgc ataggatgag agtggctatt gccttctgag tagtgtacgc aaggaatttg 70260

tctccaggtg ataaaacaac tttgtcctcg aggagggaaa acagcgtagc acagagagct 70320

cattaggatc acaagtgaac cttgaaaaaa ttcactgggc agccgagcgc agtcttgtgc 70380

tggctctgat ttctgagttg cctgttaagc caaagactga ctatatatat gtatgtatat 70440

atatatatat atatacatac atatatatat atatatatat atatatatat atatatatat 70500

accccaacgg ttttgtcatc caatccagga atgtcttccc tgagtagaaa gaaaaatgca 70560

gacattcatg tagtgaacca atctgatata ggaattttct ttttttaata tcattaaatt 70620

tatttattca ctttacgaac caatatcaat ccttcctctc ctcccagaac ccctcaagca 70680

agtcctcccc catctgaact tccccgaagc ccccgactct gtatcaaata ccacccacac 70740

acgctctccc caccacctac ctacctaccc actctccacc ctctccccca tatatcaagt 70800

tgctgcagga cttggggcat cctctaccac tgaggccaga caaggctata tatactttag 70860

gggcggagaa tccaaagata gacaggcatg caacaagctc ttggacagcc tttgtttcag 70920

tccttagggg aactccatga aaaccaagtg gctcatctac tacatatgtg cagggtgtgt 70980

aggtataaag catgcttgct ctttggttgg tgactcagtc tctgggagca tctgagggtc 71040

cactttagtt gactctgatg tataaatttt caatgctctt aattttcgta agaaaagagt 71100

tctatcaact aacacccccc cccccgacct cccagggact aaaacaccaa ctaaagagta 71160

tacatgggat ggggggaaca cgtgactccg catatgtaac cttatcaata ggaaaagagg 71220

cccttggtcc tgggaaggct ggataccccc agtatagggg aatgccaggg ctgagaggta 71280

ggagtggatg ggtggagcag caccctcata gaagcagggg gagggaggat gagatagggg 71340

gtttccagag gggaaaccgg aaaagtggat aacatctgaa atgtaaataa ataaaatata 71400

caataaaaga gaatagttgg gctggagaga tgatggctca gtggttaaga gcactgactg 71460

ctcttccaga ggtcctgagt tcaaatccca gcaaccacat ggtggctcac aaccatctgt 71520

aatgagatct gatgccctct tctggtgtgc ctgaaggcag ctacagtgta cttgtatata 71580

ataaataaat cttaaaaaaa aaagagagag agaatagtta tgatgagatc tggtgccctc 71640

ttctggcttg caggcataca tgcaggcaaa acactgcata ataaatcgat cttgtttcac 71700

aaaaggaaag aaaaaggatt taaactaaga gacagagcaa aatgttatgg aacaaaattt 71760

gaggttggct gaaatgtgtt tagagatatt tggtatggag aatttgtaac atagctcagt 71820

ggtaaatata gattcagtca tcagcaccac ataaaaaggc ataaaaattt aattagactt 71880

tctgcatcat tcacaagcac tgtttcttat aattatgaaa gaggtaggat attttacccc 71940

cacgatattc ttactgcagt attccactct tcatatatat atatatatat atatagtgga 72000

tttatcattc agcaagaatc atgatcattg aaacaactta atttgttaga agaccatggt 72060

gtgcgaagca gacgcacaca gaacaagaaa aacagctctt cctgtcttcc tctgaagctg 72120

aaacaggttc gggtctggca gtggtgactg gtatgcttgg tatgaggaaa tgcacaccta 72180

attccacatt ttttgttttg ttttttttac aaatcggagt ggttcacaat taggaagact 72240

aacttttgtc tgagcagata ctcttttttt ttcttcattt tgttttgtct aagcaagggt 72300

cacaatctgt aaaccaagca ggctttgaac tcctggcaat cctcttgctt taatctcctg 72360

agtgagtcac caggccggac tatttttctt ttgtttttga gacaggggct cattatgggt 72420

atagcatgaa atttcaactt ttgagggcac agcattaaaa ctagaggaag tgctgtgcat 72480

ggtggcgcat acattgaagc ccaacactta cgaggcagag cagcaccctc atagaagcag 72540

ggggagggag gatgagatag ggggtttcca gaggggaaac cggaaaagtg gataacatct 72600

gaaatgtaaa taaataaaat atacaataaa agagaatagt tgggctggag agatgatggc 72660

tcagtggtta agagcactga ctgctcttcc agaggtcctg agttcaaatc ccagcaacca 72720

catggtggct cacaaccatc tgtaatgaga tctgatgccc tcttctggtg tgcctgaagg 72780

cagctacagt gtacttgtat ataataaata aatcttaaaa aaaaaagaga gagagaatag 72840

ttatgatgag atctggtgcc ctcttctggc ttgcaggcat acatgcaggc aaaacactgc 72900

ataataaatc gatcttgttt cacaaaagga aagaaaaagg atttaaacta agagacagag 72960

caaaatgtta tggaacaaaa tttgaggttg gctgaaatgt gtttagagat atttggtatg 73020

gagaatttgt aacatagctc agtggtaaat atagattcag tcatcagcac cacataaaaa 73080

ggcataaaaa tttaattaga ctttctgcat cattcacaag cactgtttct tataattatg 73140

aaagaggtag gatattttac ccccacgata ttcttactgc agtattccac tcttcatata 73200

tatatatata tatatatagt ggatttatca ttcagcaaga atcatgatca ttgaaacaac 73260

ttaatttgtt agaagaccat ggtgtgcgaa gcagacgcac acagaacaag aaaaacagct 73320

cttcctgtct tcctctgaag ctgaaacagg ttcgggtctg gcagtggtga ctggtatgct 73380

tggtatgagg aaatgcacac ctaattccac attttttgtt ttgttttttt tacaaatcgg 73440

agtggttcac aattaggaag actaactttt gtctgagcag atactctttt tttttcttca 73500

ttttgttttg tctaagcaag ggtcacaatc tgtaaaccaa gcaggctttg aactcctggc 73560

aatcctcttg ctttaatctc ctgagtgagt caccaggccg gactattttt cttttgtttt 73620

tgagacaggg gctcattatg ggtatagcat gaaatttcaa cttttgaggg cacagcatta 73680

aaactagagg aagtgctgtg catggtggcg catacattga agcccaacac ttacgaggca 73740

gagacaggac atctctgagt tcaaggccag cctggtctac aaaataactt cagaatagcc 73800

agggctacac agagaaacct ggtctcaaac tacacccaca tacacacaca caaacacaca 73860

cacacacaca cacacacaca cacacacaca cacctgcaaa aaactagagg gaacttgatt 73920

tttaaaagtt aattgcacag cattttccta aacaatagta tgtttagaga accaaagttg 73980

gtgatgggtt gatatgacta tgataatgaa taacaattat ccattacaga aagtccctgt 74040

tttgctgtgc ttggtggcac atatgtaatc ccagcccttg agaaggcaga ggccggtaga 74100

tctaacaaat caacaacaaa aacgatcctt tctcttagtg gattgattac aaaagggctt 74160

tgcccgaagc ctgcataggc tagactcttc cagtctgcac ggctgtacat tgtgaacttc 74220

aactaaaggt gacctcttcc agggaggaga gtgacagtgt ctatgagtca gcagcactaa 74280

aagactttga catgggctat gttctcttta gccattcttt ggtttatgct tctatttaaa 74340

gggaggttca aaaggcattt agcaaaagtt tcaggaccct gaacgatggc tcagtaggta 74400

aagcattgcg tagcaagcct gctgagttga atttgatctc cagagtgcat gtaaacatgg 74460

atggagagaa ccaactcctc gaaattgttc tctaacctcc atttgtgtgc ccctgccgta 74520

acaacagtga ttcaatttgt tttaaagaat atttagcagg ttgccagact tattcctagt 74580

tgccagtgaa aaattgaagc atgaacattg taagcagggg agattttgga aggagacaaa 74640

tttgagaata acagaagtat agagacagat tttaaggacg ctcattcatt cacattatta 74700

aaccagtata gaaagcttac atttgggctg gagagatggc tcagtggtta atagcactgt 74760

ctgttcttcc agaagaccca ggttcgattc cacatggcat ccacatggca gcttacaact 74820

gtctgtagct ccagttccag gagatctggc accttcgcag acatatatgg acacagaaca 74880

tcaacgcata agaaataaaa ataaatcttt tctaaaaggg gaaatattat ttttgaaaac 74940

attaacatat tatccaggtt ctgcgaaggg cggtgaagcc caaataggac aatcattaaa 75000

aagccctttc tgagtttgct tcctcttcta tctccttcac ataactgagg acaaaaccct 75060

ggcacttgcg tggacacagc gctagtttca ctttttttgt tttgtttgtt taagacaggg 75120

tttctctgtg taatagtccc agatgtctta gacctcacat ggtagaccag gctggcctag 75180

actttggaga tcagtctgtc tctgcctcca gagtgctggg actaaaggtg tgcatgctca 75240

ccgcctagtg aaatttccca gttttaattt ttatttttag acaagttctc actgtcttgt 75300

tcaaggtggc cacgaacatg caatcgttct gtttcctgcc ggctctttct tgaggtaaaa 75360

attttctatg cagccctagc aaccaggaac cccttttgta gcggggctca aacttgtata 75420

gatcctcttg cctctataca aatgtgccca ccatgctttg cctttgttgt ctacattctt 75480

tcttcctttt tcgactagtt tgttgagtac gctgcatcga gcagtagact atacagtatt 75540

aataatattg cctacatcat acttactctc cttgggaatc atctctgcca ccaggataac 75600

acatttttcc tttgttactc tgttgctttg attgagtaaa cttttagcta ccacatcata 75660

ccacaaaata gttttttggt cgagtcataa atttttgtct gtgtgctaag aatcgtggtt 75720

caagaagggg ctgtccatca gcctcttctc tctaagaaaa gaacaaagct atgcttcact 75780

atgaggaatg aacagtttta cctgtgcctt ggaactgcaa gttttttcac ttaaaagtgc 75840

attcaatgca tgcagccctg aatgcttttg gcgagtgtgt gaacttgacg cttcatcttt 75900

agtaacagta ctgtagcatt gcagaaaccc aagccagttt tccccttaac attcactgcc 75960

tgtggaaaac actgtaggtg acagacaggt aaaacggagt tcaatgtgct tgttcttgtg 76020

tcctcttgaa aagtgtgaat ctgcagcctt gttttcattg atagattttc caagaggtaa 76080

agagcagtga cattgctaaa accttccgca aagccatcaa caggaaagaa gggatttgtg 76140

ctctgggagg aacttcagag ttgtccagtg aaggaaccca gcattcctac tcaggtaatc 76200

atggcatggg tgtacttggg tcagtacagt gcactgggca gatacagact gaagccctaa 76260

aaagcacgtt tctctaagta aatgtactaa ggagtcttag tgacctagtt gggtcttggg 76320

tcacatattt gtcagaaagt tcggtgatga taatcttcat tacagtggag gtgtgcaata 76380

atataaatac aatttttata tattttataa cttccctaaa gatcagttct tttgtttcta 76440

ctagcatatt aattaatccc taatggccac tggattgcct tcacgtctct gagtattcaa 76500

agaagatggc cacataattg tgaagcatta tctcttaaca gcactacaca acaagtgtag 76560

ggttggggcc aaataattaa cagagagtat gaactgattt cttgcttact taatataatt 76620

ttagaggaag aaaaatatgc tttcgttaat tggataaata aagctttgga aaatgatccc 76680

gattgcagac atgtcatacc catgaaccca aatactgatg acctattcaa agcagttggt 76740

gatggaattg tgctctggta agagactagt tttgttttgt atcccaatct ccaaagataa 76800

tctccacaga ataactgtac ttggttatgt ggttatttgg ctgtttgata tctagaaggg 76860

gggcatcaat tgctcaggtt tgtctttttc atttaatatc taaatctatg tgtaaaagta 76920

aaagtcagtc acagagtggg gacctctttt atagggtaga atttttggct tccaatttct 76980

cattactgag aaagcagaac attacatttt ttaaaaaaaa atgtaagtat gtcactatct 77040

attctgaaat accttgactt ctcaagggag aataagaggc tagccttcag ttctaactct 77100

ccagtgatct gattctgaaa actgctttcc tcactttagc tacatctgaa ccagcagtat 77160

cttggcagga gtgatgtaag attatcactt gtctgttgct gctcttgttc tcagagatgg 77220

ttttgccaca ctgttctaaa ataagctcat tattgaagtc tgggttccta tgtaaggctt 77280

cccacacagt agctgtgtgg atggggtcca gatagtcaga accttttaca tgttcatggc 77340

cttggtttcc aaactcagca catgagagca aatgcctgta atcccagcac tcatggaatg 77400

gggtcagaca aatatctgtg agttcaagga cagccaagga gacagggaga tcctgtccca 77460

aaataaaaaa gaaggaagga aggaaggaag gaaggaagga aggaaggaag gaaggaagga 77520

aggaaggaaa gaacagaaaa acaaaagata gaagaaaaag caacaacaga gctaacctgc 77580

caggcagtga tgacacacac ctttaattcc agcacttgga tggcagaggc aggcagatct 77640

ctataagttc aaggccagcc ttgtccacaa agtggatagc caggactaca catagaaacc 77700

ctatcttaaa acaaaacaac aggggttggg gatttagctc agtggtagag cgcttgccta 77760

gcaagcgcaa ggccctgggc tcagtcccca gctccgaaaa aaaaaaaaaa aaggaagaaa 77820

aaaaaacaac aaagcaaaac aaaacaaaat taaaaaccct cacaacctta gcatcacaaa 77880

aaaaacccaa aaagtaaaac ttagatttgg gttatgtatg tatgcatgtg tttgtgtgta 77940

tacatacata catgtacata tgtatatatg tgtatatata tgtatatgga atatgaataa 78000

ttttttcaga cctgggcacc ccaaaatgtc atcatatttt atctaatctg cagtttggac 78060

atatatattt tgtggtcaac gtgataaaca tcatctgtga cattttgttt ttattttatg 78120

tgtagcttca tgggtttatg tataccacct tgtgcacgta cctgtgaagg ccaaaagaat 78180

gcctagaatc ccctgaaact ggagttaaca ggcagttgtg agctgttcag tataggtgct 78240

aggagccaaa cctgggtcct ctacaagagc agtaatgttc ttaactcctg ggctatctct 78300

tcagccccag atcgagtgca ttttttatat tgcatgaaag atagtttata ataagcttta 78360

acattcatga agatttatca aagtgttgtt gttagagaca ggatttcgta tgtctcaagc 78420

tagcctcaac ttgatatgta gtccaggatg gcctcgaact cctgattctt ttgcctccat 78480

gctcttgtgc taggaatata tatttctcca tgtcctagtt atgtggtgct ggtcactgaa 78540

cccatgacct catacatgct aggtaagcac tctacaaagt gagatattaa caccgaactt 78600

tgtggactct ttcaaagtaa accaactttt gtttgtttca gcaaaatgat caacctttct 78660

gttcccgata caattgatga gagagcaatc aataagaaga agcttacacc cttcatcatt 78720

caggtgtgag gagttctcta ttctctcttc catgaactgt gatttgaaac agagaccata 78780

cactgtgtat tctgtttgct ggcctttaat ctttagagca gctccgcgtg tggaatgatc 78840

attgatgttt cagtgagctg gaactcagaa aggattgcgg ggcttgctgg aaattaacac 78900

tggcactttt gaactcaaag ccctgtgttc ctttcattac ctattgagtt aagatatcac 78960

caaaatgaac aaaatgatca agctctttct tagctggtgt gcttaaaggg tggagcgttt 79020

ttggttttcc actgaataag tagtagatta gtacagcgaa gtgccatcag gcaggagtga 79080

ccttgagata gatagattgg tcatttctag aacctggatt tatgccatat actgttggac 79140

tccaaattta ggcagttttg gaaaaaataa caccatggat atattgactt tggtgacttg 79200

acatttttac tttagaattg gcataattcc aaagcaacca ggtcaaaaga aaaagtccat 79260

tccctgtaga attctgcaca aagattacga gtgaggctga ggattgttgt cgctttgctg 79320

cttattgccc aactctgcca tttgttttct gtaaatgtgg gtggatgaaa ttaatacctc 79380

ttgctaattt ctcaactgag cggtattttc agtgaaggct gaggtggact gggtgtggaa 79440

aaaaaaaagg tcaagaagtg cataatttgg tctgtcttcc aggaaaactt gaacttggcc 79500

ctgaattctg cttctgccat tgggtgtcac gttgtgaaca ttggtgccga agatctgcgt 79560

gccgggaaac ctcatctggt cttgggactg ctctggcaga ttattaagat tggcttattt 79620

gctgacattg aattaagcag gaatgaaggt aatggaacac agttgttttt tgaaagatgt 79680

atattgttga tttttacttt ctcttcatga aaactgtcat gcttgcactt aactgagttg 79740

agcagatgga aactagaata aatccagagc tattcagaaa aacaacctgt acatacacct 79800

ctgttcttta tttgtagcta atatgacagt tcacattgga tttttctcta aagacaaaaa 79860

tacacagaga agttgatatt aagtgccttc cttagtctct ctccacttta gaattgtgtg 79920

tgtgcatgta tttttttaat ttacttatta tatttatata agtacactgt agctgtcttc 79980

agacacacca gaagagggca tcagatccca tttcagatgg ctgtgagcca ccatgtggtt 80040

gctgggaatt gaactcagga cctctgcaag agcagtcagt gctcttaatt gctgagccat 80100

ctctccagcc cctggtcttc atacttaaac tgcaagcaat ttaccaagct cttctctcca 80160

gccccaggcc tactcttgag acaagatcta gcactaaatc tggagcttgc aaatttggct 80220

agattgccag acagcaaacc ccagatactg gcacacgctc caccccaccc caaccctgct 80280

ttcacctctg cagctctaaa gggtagagac ttgtgctacc acgtctggct tttcacatgg 80340

gtgctagcag ttgaacctgg gtcctcatgg tttcacagca agccctttac tgactgagcc 80400

atgtctttag cccagagttt gaggacctgg aagttaggtc tctattacca aagtatcaaa 80460

agactgaaat gacgtatgat gcctcatgcc tgtgatccca gcatttggga ggttgaggca 80520

gggagatcat gagattaggg gaagcatggg aatgtttcaa agtaaaatgg taggagaaag 80580

ggagaaagag cgtgctcaag tacttggcat gaccactagc acacaatata ctgactgagg 80640

catttctgtc tacaagcact ggcggctttg cttcgagatg gtgagactct ggaggagctt 80700

atgaaattgt ctccagaaga gcttctgctg agatgggcca acttccatct ggaaaactca 80760

ggctggcaaa agatcaacaa cttcagtgct gacatcaagg taactatgca aagggccaga 80820

gcattcaggg agatgtgatg gcttcagaga ttttttttat cccattttaa cagagacaaa 80880

aattataggc tatcaccagg cccatgagag acaaaagtct ctcatgtttt gagataaggt 80940

cttgctatgt agctcaggct atactagaca tgacttttgt agacaatgct agcctcaaac 81000

ttactgtgat tcttcttctg cctcttgaac actggtatac agagagatac caccatagct 81060

ggatgattat ctttccatac actgatgttg tcgtttgctt ttgtttttgt ttactaaaga 81120

atgtctccta tgttccaaga tggcctcaag tctgccatgt agttaaagat gatgtgagat 81180

gctgtagtca cattttgtgg gatgttcagg attaaatcca gggtttcctc cattcaaggc 81240

aagcacacta ctaactgtgg ccattatggt tttttcaaat gtgatttgac tctgtagccc 81300

aggtcggctt gtgtcaacca ggctggcttc aaaactgcaa tcttcctgcc tctggctaaa 81360

aactatatac aggcatatgc tgaaatgcct agtcagtgga aatctttatt aggaattctg 81420

ctttgaatac ttttgaaaat caaagaatgc ttgcatttat tgaccatgtg aagccttaac 81480

ctcaatcccc atcactgtgg gagacggggg agagacgagc atgttttgtt aagtgagttc 81540

tgaccttaaa aggttaaatt ttatcttgcc aactatatgt gtttctcatt taagcttttc 81600

taaatttcaa ctttttatat aattaaatat ataattaaca taacaagtgt tgtttcctta 81660

ggtcgatacc catcttgctt tgtaatgtgt ccattttctc tttccctatg cttcctggat 81720

ttctgctctt ctatttcatg gttgtttatt tgtcaagctc cttgacttca gtaattcagt 81780

gaaggtacag aaatgcttaa tagctttatt gggttgatgg catcaataaa ctgtaaagtc 81840

agaacaaaat caagctcaaa gagaggtgga aagccagaca gatggacttt tcctgggaga 81900

aatatctcaa ataagaagac agagaagaat gccatatatt atcgccaaag ctgatccaaa 81960

cgtcctcgtc aacaagggac aatacgatgc agttactctt tgtaaatgtg ctcacactgg 82020

tatgtttgat tcttttgtag gattccaaag cctatttcca tctactcaat caaattgcac 82080

cgaaaggaca gaaggaaggg gaaccacgga tagatattaa catgtcaggt ttcaacgtaa 82140

gtataggtgc cttttcaaat tctagtcttt tcccattgtt tctttttttt ccctttggtt 82200

tcgatgaggc aaactcttgc tacgtaatgc tgtaacctgc ttgtattaga ctcatagaga 82260

gtccacctgc ctctgttttc tatgtattgg gtctaaagct ttgtgctccc atacccaaat 82320

aattttgcat aaattctatc aaaatgttta tgtaacttca tgtagtggtg tgtggtgttg 82380

acacttttaa ggttttttta aagaggattt gatacatgtg actcttagaa gagaaagtat 82440

cctgatttat tttgataact tgtgaggtcc ataattttga tggtagtaga ttttatgaat 82500

gacataaaca ttcaaagaga gtatggggga agaagcttat tccccacggt aggggtaaag 82560

ggctagtcta tgcattaagg gaactatctg tggtagcttg tcctcttgaa ataaaattac 82620

agtcatgctc agaggtgcgt ctccaagttc atttgaaatc aaggtgagtt gacagctaag 82680

atgatccatc ccaacagcaa atcctccctg aatgatgttt gattcatccc ccaaaatcca 82740

tccaggcttc ccctactggc tctagaattt ggtctctctg cttagcattt aaattcctac 82800

aaaatattac tcatcacttt cagtctgccc caaaaacctc ttttcacctg catatagctg 82860

tcttagactt taccaaacat tcctcccctt tcagtttttt aagacagggt ttctttgcat 82920

agccctggtt gtcctgaaac tcaatatgta gaccaagctg gccttaaact cagagatctg 82980

cctgcctcta cctgcctcta cctcttgtgt gctaggatta aaagcgtgtg ccacaaccac 83040

ccagcccaaa catccctttt aagtccaagc ccatgcttgc aagttttttc agatatgttc 83100

tttaagtaaa aaattaaggg tctggagaga tggctcagcc aagtacttgc tgctctagca 83160

gagtgctctt tttaggcttc caacctttaa ctccagatct aggagatcca aatgccttca 83220

tcttctattc aggcatttgg gaatgagaga gatttaaaaa aagctgtttt gaaaataaaa 83280

tgtttttctg aaactatcca tatttgaaga caagtcttca aaggttgggt aatggcttca 83340

ttccctttgc acgttgccat gagccttaat ctcaatggaa tgctttattc agtccttaga 83400

tgtattctgg cgccatcttg gggaatttaa aagtactaca tattgcatgc ctcgttttaa 83460

cattcaaaac caattttata agtaccgtgt gttagctgaa agagctatgc tttacccaac 83520

ctatgattag attatagatg aaatgtgtgt gtatatatac atacatacat acataagtac 83580

atacatacat agatgatggc ctttaaaata tgtcttcatt ttcttcaaca ggaaacagat 83640

gatttaaaga gagccgaaag tatgcttcag caagcagata aattaggctg cagacagttc 83700

gttaccccgg ctgatgttgt gagcggaaac cccaaactga acttggcctt tgtagccaac 83760

ctctttaata aatatccagc actaactaaa cctgagaacc aggatattga ttggactctt 83820

ctagaaggta actcaaaggt cctcgaattc gcatgtgcag tctgtagcat aaaaccccag 83880

aggccgcaat gatgtcattc caacccagtg agttgcttta tccatttggc ttttgaaaga 83940

atctgttaga agcagatgcc gtgctgagtg agaactggaa atggctggat ggagggagtg 84000

atggaagcaa agtatgcaaa taaaataatg gagtggggga atgtggctcg gtgccagagc 84060

tcttacctag cacggccact gctacaaaag aaactagagg gctggagaga tggctaagtg 84120

gttaagagca cccgactggt gttccagagg tcctgagttc aattcccagc aaccacatgg 84180

tggttcacaa ccatctgtaa agagatctga tgccctcttc tggtgcgtct gaagacagct 84240

acagtgtact tatatataat aaatgaatga atcttaaaaa aaaaaaaaaa aaaagaaact 84300

agaacatcca gagtcacctg atatgagaac acttagtaaa caaatgtttg gctccaatgt 84360

ttagtgccat atctttctct cttttttgtt tgttttaaac ctccttattg attttgagcg 84420

aagagtctca tgtattgctt ctttgagatc tttttaacct agtcatctat tttgggacaa 84480

gcatttaatg tgtctccagt tgacctcaaa cttactacac agtaaggaat gaacgtaaga 84540

ttttgatgct cctgtctcca agtgctggga ttacaggcat catttgtggt tttgagggtg 84600

ttagggagta aacctggaac ttcatgcact ctaggcaagg actctataaa ctgagcttca 84660

ttccccagag cctctgagat agttgtgaga cagggtctca ccctgtagcg gtaagctggc 84720

ctgaaacaaa ctatgtagcc caagactggc ctcaagctct tggtgatctt ctttcttcag 84780

ccaaatgagg gctgagatta aagatatgag ccaccatgcc tgactagacc acagaaaggt 84840

ataaatacat acagaattta acgtataagc ttttgtaaac atatttcatt tatccataga 84900

gacatcctag catgcaatga ttattagctc agaataaagc atatcaaata aatggataga 84960

tgcagtggtg ttgtatgtac gagacaatga attaatgaat atattttgtg aatgttttaa 85020

taggagaaac tcgtgaagaa agaacctttc gtaactggat gaactctctt ggtgtgaatc 85080

cccatgtcaa ccatctctat gtgtaagctg ccttactgct cttaggaagt tatgaatatc 85140

atgaatatat gtagcataaa taatgacaac tataaaattt ttctacatgt tcgtttatag 85200

caactattcc tattatgaag cgttactgat ttctattatc gttttccaaa tacacacgaa 85260

cccaaattct ttctttgcct acctattggc tgtatgtgat agaatataag tgtcttgatg 85320

gtacttcagg gaaaaggtac tttaagtttc ttaaatagtc caggaatatt cataacagac 85380

tacccatgat tccctacagt ctttggcttc ctggtcggac tgttctttgc tgacatacag 85440

tggtttatat cctaacaata ttccctagct tcattatttt cctaccatct gccattcact 85500

acctatgaag tctactcatt gcatttacat atatgtcaaa atcatactat ttgcttatct 85560

acacatcttt ttgactctcg agtagggcct tgggttttca tcttgtgttc tccaccttta 85620

taatattttt tgcttgcaat aagtgcacat ccttacatca tcgtcatcat tttggttttt 85680

caagacaggc tttctctgtg tagtcctggc tgtcctgaaa cactctctgt tgaccaagct 85740

ggcctcagac tcaaagagat ctgcttggct ctgcttccca agtgctgtaa ttaaagacat 85800

gtgccactac tgcctggccc ttatttttta agacaggaca gcaactttgt ggccctggct 85860

gtcctggaac tggctataca gaccagtttt aactcactca caaagatcac cctgccactc 85920

cctcccaaga ctgggaccaa aggtgtgtgc caccttgccc agctgttctc acacactatt 85980

aagtaaactg aatggtcttg gctctatttt cagaacaggt atatgagtta taggaactgt 86040

gactttatag atcctgtgtg atttgtatca ctgcttacaa acactgatga agttatgttt 86100

caaattgtta gtgacctgca ggatgccctg gtgatcttac agctgtatga gagaattaaa 86160

gttcctgtgg actggagtaa agttaacaag cctccatacc caaagctggg tgccaacatg 86220

aaaaaggtag agaaccttga atcttcttga ttactttctt ttcctttaaa acctgtagac 86280

ccgggctgga gagacagctc agtggttaat agcactggct gctcttccag aggaccaagg 86340

ttcaataccc ggcaactcca tggcagctca caattgtctg taactccagt ttcaggcctc 86400

tgaaaccaca cagacataca caaatgcaaa tcaaataaaa ataaaaatga tatatagata 86460

gatacataca acatacatac atacatacat acatacatac atacatacat acatatattc 86520

atatatacat aaaaaacctc ttgagcctaa gttgcttctg gaggacaagg acaagaacta 86580

gatcttggct ttgagcagat acttactatt atacaataca tgcgtgagcg tgcgcacaca 86640

cacacacaca cacacacaca cacacacaca cacacacacg catgcatggt gggattatgc 86700

agattgaaca aatgacaata tttaaccatt actttgcttt tgtcgatttg cagttagaaa 86760

actgcaacta tgcagttgaa ttagggaaga atcaagctaa attctccttg gttggcattg 86820

gaggacaaga cttgaatgat gggaacccaa ccttgacctt ggctgtagtc tggcagctga 86880

tgagaaggta ttgtacacag tgttgactat taaagcttca cagttatatg cctatatgaa 86940

ttcaccctta gtatattttc actgattaaa ttaatcattt agttcaatga atacaattca 87000

gagctcattg tacttagact ctgaaatgag tgtatgttgg atcttcactg ggagataact 87060

ccttgtgagt taggtagaat cgtagctatt gccagtctgt cacagaagtc tacatgtgta 87120

gatttaagcc tgatgcaatg tcactgaata acattaacag acaatctaat gtagttcact 87180

gactaaaatt gtcagagggt gtgcagctct agcttagtag tctacgatat acttagcatg 87240

catggaggct aacctcagag cccccagatg aatataagga attgacagaa acccaaaatg 87300

gtggctcata cttgaaatgt cagcacctag gagtctgagg ggggagaaat ggctagtttg 87360

aggccagttt gggctacata gtgaattctg gggtatcttg aagtataacg tggaaccttg 87420

cctcaaaaca acaaaccaaa aacagtatca gaagaaatat gaacatctgt atgtatccat 87480

tttgaaatga cgaacagggc cagcaggata gctcagtgtt aaggatcctg tgcccagctt 87540

gatggcctga gttctgttcc aggaacccat gtggtaaagg aacaaaacca agtcccataa 87600

gttgttcctt gatttccata ggggtaccat aacatacatg tgttcaaata catataaata 87660

attatagttg ttataattct ttaaaaaatt gaagattcct ttatcttgat ccgtttttaa 87720

taattctttg agaatttcat acaatgagct ctaataatac ccaatcccac ttccagcccc 87780

tccaaccgcg ctggattccc ccacttttct ttcctacttt tgttataacc tactgaatcc 87840

cattaatgtc tcccatatgt acagtgtgga atgttacatg tgaagcatag ggaccaatta 87900

aaagccacat tccatgataa aaaacttact ctgtcaccca gtcgtatcca ctgtcaacac 87960

taagagtgag gcatcatggg tcctttctct atccatgctg taatgctgtg tcttgatctt 88020

gagtgggtct tattcaggca gtggcagcca ttgtgaattg atgtgggcaa acagacctgt 88080

cctgaatctg gaagcagaag ctggtgcaga ggccatgggt cgcagggggt ggggcatgct 88140

gcttcctggc tttctcatca ctgcttgttc agcctgcatt cttatataac gtagcattac 88200

cagctctaga accacccaca agggacggac cttcccccat caataactga ctaagaaagt 88260

gactacacca ggtcgtatgg agacagtttc tcaattaagg cttcttcctt tcagataact 88320

gtattttgtg tgtcaagttg acacaagact agccagcaca gaaagagaga gaaagagagt 88380

agagaataga ggatgacctc aggtgtcatg ttcaggaaca cgatccacct gctttgagat 88440

ctgcctacta tcccgagata aagttagagc ctttcatagg gaattgggca tagctctttg 88500

attagatttg acctcaggtg tcatgttcag aaataccatt cacctgcctt gagatctgcc 88560

tactatctaa gataaaggta gagcctttca aagggacttg ggcgtagctc tttggctagg 88620

ttggttggcc agtgaacccg ggaatctact gtctccacct gcccagcact acaattacaa 88680

atacattcct gcatgcctgg catttcttta tataggtgct cgtgtttgaa tccaggtctt 88740

catgtttgtg tttgtaactc aagcacttta ccgattgagc tatttcccaa ctcctccttg 88800

atacacatac acacacactc acacacacac acacacacac acacacacac acacacacac 88860

acacacaaac atggctcttt gcatgaagtt acttttattc tgtgtgtgta tactaatttg 88920

gtttaagtat ataatttcta tgcatgtgta taaactgtat gtgtgagaat gactaagtat 88980

agtaaggctc atactactgc ctcagataga cgtcagagta taagtttggg taataatttt 89040

tcactatcta ctatgtagga atcagattca attttcacac aatgtattag gctgttttac 89100

ccactgagcc atcttgctga cccctataaa atctatgtga tacatatgta cataaggtat 89160

atttctaatg tttgcatttt tcttgataga atgcatggtg actttgttct gctgcttctc 89220

ttagatatac ccttaatgtt atggaagatc ttggagaggg tcagaaagca actgatgaca 89280

tcattgtaaa ctgggtcaat ggaacattga gtgaagctgg aaagtcaact tctattcaga 89340

gttttaaggt cagaatactg attattatac attatgtttg ctagataata tcggctattt 89400

tacggacaac cttgagtttc tttgtaagag ttatcattgt tgagctcggg gcagcatttt 89460

cctttccaag gtcaggctaa caaaaagaag cacgtgatca tttgtaaata ttggaattct 89520

taggcttgtt ccctttttca taataggaca agacgatcag ctccagtttg gcagttgtgg 89580

atttaattga tgccatccag ccaggctgca ttaactatga ccttgtgaag actggaaatc 89640

taacagaaga agacaagcac aataacgcca agtaagggcg accatgtgtg gtctctcagg 89700

gtcactcaag aacactggtg ttccagccag gcagttctac tctaagtcaa gctctcccac 89760

ttgttagtga ggacgttagg ggaaagatgt cttctctttt aagggtttaa ctttactttt 89820

tgtcttagtt agggtttcca ttgctaagaa gaaacaccat gaccaaagca actctttttt 89880

taattttatt tttcttgtat attttatgta tttacatttc aaatattccc cttttccccc 89940

tctccctgct tctatgagga tgctcccatt cctacccacc tactccaacc tcaatgaaag 90000

atttatttat tcttatttat atgagtacac tgcagttgtc ttcaaataca ccagaagagg 90060

gcattgaaat ggttgtgagg caccatgtgg ttgctgggaa ttgacctcag gacctctgga 90120

agaacagtca gtactcttaa ccactgagcc atctctccat ccagaccaaa gcaactctta 90180

taaaggataa catttaattg gcactgcctt acatgttcag aggttcagtc cattatcatc 90240

aaggcaggag catggcagca tccaggcagg catgacactg gaggagtaag agtatacatc 90300

ttgatccaaa cgcatccagg agaaaactgg catcctctgg cagctaggag gaaagtctct 90360

cctgcaatgg gtgaagcctg agtgtaggag gagatcgcca aagcccaccc ctacagtgac 90420

acacttctaa caatgtacac ctcctaatag ttaccactcc ttatgggcca agtgtattca 90480

aaccacgatt ttaatggctt tactgagatc caactcacag ataaacaatt cactcattta 90540

tgtatataca atctaatgtt tttgaccttg ctcacactga tatatgacca tcaacacaat 90600

cgattttaga acactgttat cagataaaac agaaccttcc tgagtaccca gcaaacctat 90660

gttctagttt ataactgcct acccttaact ctattttgtc tctctgctct ttaatatcaa 90720

ttatatagcc acttcaccaa catctccccc gtaaaccttg acattacagc tcgcatctct 90780

gtccttttgt gagaatagct ttggagagag cccatatggg acaatgaaac acaatgggac 90840

ctctagtaag aatgcaatgt tctcagttgt aacaacaagg gtgaatatga agatgatgtt 90900

aaatttcaga cacagaaata gcagtatggt ttgatattag atctgtgagg ggaagcatcc 90960

tttgtttgtt ttggtttagt tgcatttgtt tgtttgtttg ttttgataca aggtttctct 91020

gtgtacgtat ggctgtcctg gaactccctc agtagatcag gcagacctct gactcaaact 91080

caagagatct gcctgtgtct tcctcacaag tgctagaatt aaaggtgttc acaagaaata 91140

tcattataaa cctatattgt tattgtatgt gtaggggtgt tttgtctgca ggtatgttgg 91200

tgtaaaactt gtatgtgtgc ctagtgtgtg cgtgaatagg ccagaaaagt gtgtcatatc 91260

ctctgaaact gtagttctag actgttgtaa gctaccatgt aggtacgtgg aattgaacct 91320

gagtcctctg gaagagcaac cagcaaccca tataaggcaa atatctttaa accacaaatt 91380

tatcatacaa aaataaatct aatttttacc ttaaaaggtt caaatgttta caaatatttt 91440

aagaatctgt tatctcatta cactgcacag aggatttcat aattgattat gcagtcagtc 91500

cggctaatta cacacacaca gacacacaca cacacacaca cacacacaca cacaccccac 91560

aaatatttct ttttgttgtg ggggtaggct gtttatctgt ttgatttttc aaaatagagt 91620

ttctctgttt atcgctggct gttttggatc tctctgtaga ccagtctggc ctcagactca 91680

gaaatgttct tgcctctgcc tcgagagtgc tggaattaaa ggcatgtgcc accatgcctg 91740

gccaacaatt tcatttttat atgaactcag tgcatcaagg tttccttttc ttttcatatt 91800

tggaagaaat gagtaaatac aatccaaaat aataaattgt aaaaatgtag tctaacttgg 91860

acctggagag atggggactc aactgttaga agcacttgtt gcacttacag acaagctggg 91920

tttggtccca gcaccgaatg gtggtacctc cactttcagg aaaactaaaa tccacttctg 91980

acctcttcag tcatcaggaa cacatgtagt tcacacatgc atgtaggcaa aacacttaaa 92040

tccataaaat aaatctcatg ctttgatagt ataagttaaa aatcaattca aaacaaaaat 92100

taagcagaaa acaagtctga aattattaat ttgcactttg tgttttctga ggtaaagtgt 92160

cactgtgtac ttccgactgc tttcaaactt acagggactc tttggcttca ccttctgccc 92220

caagagcact atgattatag gactatgagt atgtgtcacc aggccccgat ctctgagtta 92280

tacattcatc tcagaaatgt tgcatcccag ccatcaaatt ctataagagg aagtaccttt 92340

cttaaaaagg tgtccttgga ggggttgggg atttagttca gtgggagagc gcttgcctag 92400

caagcgcaag gccatgggtt cgatccccag ctccggaaaa aaaaaagggt gtccttgggc 92460

tggagagatg gctcagtgtt tggttaagag cattggctgc tcttcttgag gacctgggct 92520

taagttctag tacccacagg gcagctcaca actgccataa cttcaatttt aggggatcca 92580

acaccctcac acagacatac atgaatgcac aacaccaaag cacataaaat aaaattaatc 92640

ttttattaaa agagtgcaaa gaacttcttg atggaaaaaa actcatcttg atttttgttt 92700

cctcaaaaac taggtatgca gtgtcgatgg ctagaagaat aggagccaga gtgtatgctc 92760

tccctgaaga ccttgtggaa gtaaaaccga agatggtcat gaccgtgttt gcctgtttga 92820

tgggcagagg gatgaagaga gtgtgaaata gctaattaga acattaccaa ccttactcgc 92880

aggtgcatga ttagcaagtc agctagtctc aggtgaagtg cacatggctt cagaagtctt 92940

ggtcatttaa aggactttta gttttgatta gcaagactag ttaatcatga cggccttcag 93000

agaaagcatt ttaataaaca agcagttcct ctttcctgta gacctggatc tgttcagcac 93060

acctgaacaa gctcacagcc aacctttttg ctctctcctc ctagaatgct gcccttgaca 93120

ttccggcttg ctttgtatta tctatgtatc ttttccctcc tgggatatcc atctcttgag 93180

acttgcatag atgtgtagat aggaatatta ttagctcgtc attttgcttt ccaagtgcta 93240

gcgattcttt ctgaagaact gtgctctgag gctatatgga gatgagtgtt ctttgcttac 93300

cttgtagtgc tgaaaatctg ctgaagtaac tggctgtgca gaatgtacta gaagcttttc 93360

tcattctttt attcttaaaa tcagtatctt tttacagtat tctttctaca tgattatttt 93420

tttgtacatt taagaatatt ttgattacat ccaacacgtc tgctgatttg ctactttttt 93480

aaaaggggtc ttcatgtaag taaaacatac aaaggcataa attataggtg gaggaaagtg 93540

gacccgaagg ttgcatttcc ccctcatacc caggctcaaa tggaatgaaa tattttgtct 93600

tcaataagtc agtctgatac atacttcttt gtttggaaat ctttatcaca aaactctttt 93660

tggtctacaa tgctgttgaa tatgacactt tgaggggaga gtgggctcag aatttagatg 93720

ggaatttgat aagcaaagaa tgctaaagtg tacaatatat gtttaagttt cacacctgta 93780

acaaggagat gtggtcacaa aaatgagaaa gtatgtcgga cttgatgtcc tcttgctctg 93840

tcaagctatt tgctatagtt ttcaagatca ttatgttact ctaactctga aaagtgatgt 93900

aatctggtag caacatagta gtccaaataa aggcatttgc ataataatcg ttctcaaa 93958

Claims

1. A construction method of a novel PLS3 gene knockout rat animal model is characterized by comprising the following steps:

(1) carrying out targeted knockout on a specific fragment of the PLS3 gene in a cell to prepare a PLS3 gene mutant cell;

2. The method of constructing a recombinant vector according to claim 1, wherein the specific fragment in step (1) is an E10-E16 fragment of PLS3 gene.

3. The method of claim 1, wherein the animal in step (2) is a mammal, preferably a rat.

4. The method of construction according to claim 1, comprising a knock-out of a specific fragment of PLS3 gene in a cell.

5. Construction method according to claim 4, characterized in that the CRISPR/Cas9 gene editing system is adopted in the knockout method, and the target mutation of PLS3 gene is realized by introducing sgRNA and Cas9 into a receptor cell, preferably the receptor cell is a fertilized egg.

6. The construction method according to claim 5, characterized in that the sgRNA expression vector includes sgRNA1, sgRNA2, sgRNA3 and/or sgRNA 4;

7. The construction method according to claim 5 or 6, characterized in that the sgRNAs comprise sgRNAs and sgRNA-associated biomaterials.

8. The construction method according to claim 7, wherein the sgRNA-associated biomaterial is selected from any one of the following (1) to (7):

(1) a nucleic acid molecule encoding the sgRNA;

(2) an expression cassette comprising the nucleic acid molecule of (1);

9. The use of the production method according to claim 1 in any one of the following (1) to (3):

(1) use in the study of osteoporosis and/or other bone diseases;

(2) the application in the research of tumor diseases;

10. The sgRNA or sgRNA-related biomaterial of any one of claims 5 to 8, for any one of the following (1) to (7):

(1) the application of the mutant in constructing PLS3 gene cells;

(3) the application in preparing PLS3 gene mutation animals;

(5) use in the study of osteoporosis and/or other bone diseases;

(6) the application in the research of tumor diseases;