CN112239729A - Culture medium and method for promoting efficient spore production of Arctostaphylos - Google Patents
Culture medium and method for promoting efficient spore production of Arctostaphylos Download PDFInfo
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Abstract
The invention discloses a culture medium for promoting efficient spore production of arthrobacter and a method thereof, wherein the culture medium is a sugarcane juice culture medium or a potato juice culture medium, and the method is characterized in that the arthrobacter is activated on a PDA culture medium and then inoculated into the sugarcane juice culture medium or the potato juice culture medium for constant-temperature culture for 7-15 days. Wherein the sugarcane juice culture medium contains the following substances in parts by weight: 30-100g/L of sugarcane juice, 1.0g/L of magnesium sulfate heptahydrate, 1.0g/L of monopotassium phosphate and 20g/L of agar; the potato juice culture medium contains the following substances in proportion: 50-400g/L of potato, 20g/L of glucose and 20g/L of agar. By adopting the culture medium and the culture method thereof to culture the arthrobacter, the efficient spore production of the arthrobacter can be promoted, the produced arthrobacter can be quickly obtained for propagation, the produced arthrobacter can be obtained in 7 days at the fastest, and the propagation of one generation into the next generation can be finished. The culture medium and the culture method thereof are used for propagation of the short-section rhodosporidium and can greatly shorten the propagation period of the short-section rhodosporidium.
Description
Technical Field
The invention belongs to the technical field of bioengineering, and particularly relates to a culture medium for promoting efficient spore production of Arctosporium and a method thereof.
Background
The arthrobacter is a mould which is not common in nature, 3-nitropropionic acid (3-NPA) can be generated by growth and propagation under proper conditions, the 3-nitropropionic acid is a neurotropic toxic substance, the central nervous system, the digestive system, the respiratory system and the urinary system of a human can be damaged, 100 grams of the 3-nitropropionic acid can be eaten by the human as long as the human eats less, and 1000 grams of the mildew sugarcane can cause propionic acid poisoning. The existing research shows that the tolytricha glabrata is distributed in sugarcane samples of sugarcane production areas (Guangdong and Guangxi) and deteriorated sugarcane poisoning and disease areas (mainly northern areas), wherein the distribution is respectively 7.6 percent (9/19) and 56.4 percent (53/94), the latter is obviously higher than the former, the tolytricha glabrata strain in the sugarcane production areas accounts for 72.9 percent (35/48), and the tolytricha glabrata strain in the poisoning and disease areas accounts for 7.6 percent (40/227).
3-Nitropropionic acid is biotin, and multiple injections of 3-NPA (30mg/kg, 2 injections) in large doses can inhibit mitochondrial function by selectively inhibiting succinate dehydrogenase, which interferes with the conduction of cellular electrical activity leading to cell death. Although 3-nitropropionic acid is toxic, everything is in harmony, in recent years, domestic and foreign researches find that the ischemia and anoxia tolerance of neurons can be improved when a single 3-NPA (20mg/kg, injected once) pretreatment is carried out at a proper dose, and the protection mechanism of the 3-nitropropionic acid possibly relates to links such as activation of adenosine receptors. The existing research shows that in a temporal lobe epilepsy model caused by kainic acid, the pretreatment of small-dose 3-nitroacrylic acid can inhibit the apoptosis of hippocampal nerve cells of rats suffering from the epilepsy caused by the kainic acid and the expression of p53 protein, the pretreatment of small-dose 3-nitroacrylic acid has a certain protection effect on the apoptosis of hippocampal nerve cells of rats suffering from the epilepsy caused by the kainic acid, and the protection mechanism of the pretreatment can relate to the activation link of adenosine receptors (research on the influence of 3-nitropropionic acid on the apoptosis of hippocampal nerve cells of rats suffering from the epilepsy caused by the kainic acid and the expression of p53 protein [ D ], 2006, first department of medical sciences: neurosurgery). 3-Nitropropanoic acid pretreatment can up-regulate ischemic penumbra Bcl-2 protein expression, down-regulate Bax protein expression, reduce neuronal apoptosis, and protect against cerebral ischemic injury by opening mitochondrial ATP-sensitive potassium channels (Zhengsha, xuronghua, Chengni, Lihongge, Meiyunwu. 3-Nitropropanoic acid pretreatment has an effect on apoptosis of rat focal cerebral ischemia reperfusion cells [ J ], (Guangdong medicine) 2008 12 years). The 3-nitropropionic acid can be pretreated for a plurality of times to stimulate the synthesis of reduced glutathione by reducing the generation of malondialdehyde, so as to protect dopaminergic neurons and achieve the effect of preventing Parkinson's disease (Zhuangzheng, Deng Shenjun, Sunsheng Sheng, etc.. the mechanism for protecting dopaminergic neurons by the 3-nitropropionic acid is pretreated for a plurality of times [ J ], (clinical recovery in China) No. 34 in 2006). The 3-NPA pretreatment induces cerebral ischemic tolerance, and the mechanism may be to up-regulate GLUT1 and GLUT3 mRNA and protein expression levels, and maintain energy supply to brain tissues (chen am, yand prize, daoyang, zhuang. influence of 3-nitropropanoic acid pretreatment on expression of cerebral ischemic tolerance models GLUT1 and GLUT3 [ J ], (journal of chinese pathophysiology) at 11 th 2010).
As pharmacological properties of 3-nitropropionic acid show that the demand of 3-nitropropionic acid is more and more, the production of 3-nitropropionic acid by using microbial strains is also a conventional method, and at present, some researches on the production of toxin by using Arctostaphylos sp are carried out. Luxueyun et al screened out three culture media for producing toxin by using nodularia nodularis, which are malt extract-peptone, malt extract-yeast extract and potato-yeast extract-sucrose, from a culture medium consisting of malt extract, potato juice, peptone, yeast extract and sucrose. The LD _ (50) of the three cultures of the toxigenic strains of Arctostaphylos jirima to mice were 54.0mL/kg, 55.0mL/kg, and 52.0mL/kg, respectively (Roxuyun, Liuxing _29600, Liyuwei, Li xiufu. etiological study of metamorphic sugarcane-III. study of toxigenic culture medium of Arctostaphylos jirima, sanitary study, 1986, 15(3) -25. Liu Jiang et al have studied the factor that influences the toxigenic nodularis toxinii production of the design of orthogonal test, for strain temperature > time > culture medium > pH value according to the magnitude sequence of the influence degree that influences the factor of toxigenic in the laboratory. Selecting a culture medium: potato juice-yeast extract-sucrose medium (PYS), malt extract-peptone medium, malt extract-yeast extract medium, sugarcane juice medium, wherein the sugarcane juice is prepared from commercially available sugarcane by peeling, crushing, and squeezing with gauze (liujiang, liuxingji 29600, monshoch, laboratory studies on factors affecting toxigenicity of nodystilus, sanitation studies, 1992, 21(06) -303.
The tolytricha is mainly from deteriorated sugarcane, but the cost for separating the tolytricha from the deteriorated sugarcane is high, so that the method for accelerating the breeding of the tolytricha becomes the key for culturing more tolytricha, the research on the tolricha is mainly focused on the aspect of accelerating the toxin production of the tolricha, and the research on the method for accelerating the spore production of the tolricha is very little. Therefore, the present applicant has conducted intensive studies on how to accelerate the spore production of Arctosporium.
Disclosure of Invention
The invention aims to provide a culture medium and a method for promoting high-efficiency spore production of Arctium japonicum, and the culture medium and the culture method can be used for culturing the Arctium japonicum, so that the high-efficiency spore production of the Arctium japonicum can be promoted, the Arctium japonicum producing spores can be quickly obtained for expanding propagation, the Arctium japonicum producing spores can be obtained in 7 days, and the propagation of one generation into the next generation can be completed. The culture medium and the culture method thereof are used for propagation of the short-section rhodosporidium and can greatly shorten the propagation period of the short-section rhodosporidium.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for promoting efficient spore production of Arctostaphylos metschlechleri comprises the following steps: activating and culturing the arthrobacter strains on a PDA culture medium for 6-8 days, inoculating the arthrobacter strains subjected to activation culture into a sugarcane juice culture medium or a potato juice culture medium, and then putting the arthrobacter strains into a constant-temperature culture box to perform constant-temperature culture at 27-29 ℃ for 7-15 days to obtain the produced arthrobacter.
Furthermore, the obtained nodule spore producing rhodosporidium sp can be inoculated to a new sugarcane juice culture medium or a new potato juice culture medium for further propagation, and propagation is continuously carried out for multiple generations so as to propagate more nodule rhodosporium sp offspring.
The sugarcane juice culture medium contains the following substances in parts by weight: 30-100g/L of sugarcane juice, 1.0g/L of magnesium sulfate heptahydrate, 1.0g/L of monopotassium phosphate and 20g/L of agar; mixing the raw materials at a certain ratio, and sterilizing at 121 deg.C and 105KPa for 20min to obtain sugarcane juice culture medium; the sugarcane juice is obtained by juicing sugarcane stems and filtering.
Preferably, the sugarcane juice culture medium contains the following substances in proportion: 50g/L of sugarcane juice, 1.0g/L of magnesium sulfate heptahydrate, 1.0g/L of potassium dihydrogen phosphate and 20g/L of agar.
The potato juice culture medium contains the following substances in proportion: 50-400g/L of potato, 20g/L of glucose and 20g/L of agar; cutting potato into small pieces, decocting in boiling water for 20-40min, and filtering with gauze to obtain potato juice; and uniformly mixing the potato juice with glucose and agar according to a ratio, and sterilizing at 121 ℃ and 105KPa for 20min to obtain the potato juice culture medium.
Preferably, the potato juice culture medium contains the following substances in proportion: 100g/L of potato, 20g/L of glucose and 20g/L of agar.
The invention has the beneficial effects that:
the sugarcane juice culture medium or potato juice culture medium has the advantages of few raw materials, low cost and simple preparation method, and the culture of the Arctosporium alternifolia by adopting the culture medium and the culture method can promote the high-efficiency spore production of the Arctosporium alternifolia, quickly obtain the Arctosporium alternifolia for spore production for propagation, obtain the Arctosporium alternifolia for spore production in 7 days, and finish the propagation of one generation into the next generation. The culture medium and the culture method thereof are used for propagation of the short-section rhodosporidium and can greatly shorten the propagation period of the short-section rhodosporidium.
Drawings
FIG. 1 is a graph showing the growth of Armillaria mellea obtained by culturing the cells in the sugarcane juice medium containing 30g/L of sugarcane juice of example 1 for 7 days;
FIG. 2 is a graph showing the growth of Armillaria mellea obtained by culturing the cells in the sugarcane juice medium containing 50g/L of sugarcane juice of example 2 for 7 days;
FIG. 3 is a graph showing the growth of Armillaria mellea obtained by culturing the cells in the sugarcane juice medium containing 100g/L of sugarcane juice of example 3 for 7 days;
FIG. 4 is a graph showing the growth of Arctomyces nodularis cultured in potato juice medium containing 50g/L of potato of example 4 for 7 days;
FIG. 5 is a graph showing the growth of Arctomyces altaicus obtained by culturing for 7 days in potato juice medium containing 100g/L of potato of example 5;
FIG. 6 is a graph showing the growth of Arctomyces metschnikoides obtained by culturing for 7 days in potato juice medium containing 200g/L of potato in example 6;
FIG. 7 is a graph showing the growth of Arctomyces nodularis cultured for 7 days in potato juice medium of example 7 containing 400g/L potatoes;
FIG. 8 is a graph showing the growth of Arctomyces metschnikoides cultured for 7 days in the PSA medium of comparative example 1;
FIG. 9 is a graph showing the growth of Armillaria mellea cultured in the corn starch medium of comparative example 2 for 7 days.
Detailed Description
In order to describe the present invention in more detail, the present invention will be further described with reference to the following examples. The following species of Arthrospira are isolated and stored by the Applicant from commercial sugar cane.
Example 1
A method for promoting efficient spore production of Arctostaphylos metschlechleri comprises the following steps: activating and culturing the arthrobacter on a PDA culture medium for 7 days, beating a bacterial cake with the diameter of 5mm at the edge of a bacterial colony, inoculating the bacterial cake into a sugarcane juice culture medium, and then putting the culture medium into a constant-temperature incubator to perform constant-temperature culture for 15 days at 28 ℃.
The sugarcane juice culture medium contains the following substances in parts by weight: 30g/L of sugarcane juice, 1.0g/L of magnesium sulfate heptahydrate, 1.0g/L of potassium dihydrogen phosphate and 20g/L of agar. Juicing sugarcane stems, filtering to obtain sugarcane juice, adding 1.0g of magnesium sulfate heptahydrate, 1.0g of potassium dihydrogen phosphate and 20g of agar into 30g of freshly squeezed sugarcane juice, uniformly stirring to completely dissolve all the components, adding water to a constant volume of 1L, and sterilizing the solution at 121 ℃ and 105KPa for 20min to obtain the sugarcane juice culture medium.
Example 2
A method for promoting efficient spore production of Arctostaphylos metschlechleri comprises the following steps: activating and culturing the arthrobacter on a PDA culture medium for 7 days, beating a bacterial cake with the diameter of 5mm at the edge of a bacterial colony, inoculating the bacterial cake into a sugarcane juice culture medium, and then putting the culture medium into a constant-temperature incubator to perform constant-temperature culture for 15 days at 28 ℃.
The sugarcane juice culture medium contains the following substances in parts by weight: 50g/L of sugarcane juice, 1.0g/L of magnesium sulfate heptahydrate, 1.0g/L of potassium dihydrogen phosphate and 20g/L of agar. Juicing sugarcane stems, filtering to obtain sugarcane juice, adding 1.0g of magnesium sulfate heptahydrate, 1.0g of potassium dihydrogen phosphate and 20g of agar into 50g of freshly squeezed sugarcane juice, uniformly stirring to completely dissolve all the components, adding water to a constant volume of 1L, and sterilizing the solution at 121 ℃ and 105KPa for 20min to obtain the sugarcane juice culture medium.
Example 3
A method for promoting efficient spore production of Arctostaphylos metschlechleri comprises the following steps: activating and culturing the arthrobacter on a PDA culture medium for 7 days, beating a bacterial cake with the diameter of 5mm at the edge of a bacterial colony, inoculating the bacterial cake into a sugarcane juice culture medium, and then putting the culture medium into a constant-temperature incubator to perform constant-temperature culture for 15 days at 28 ℃.
The sugarcane juice culture medium contains the following substances in parts by weight: 100g/L of sugarcane juice, 1.0g/L of magnesium sulfate heptahydrate, 1.0g/L of potassium dihydrogen phosphate and 20g/L of agar. Juicing sugarcane stems, filtering to obtain sugarcane juice, adding 1.0g of magnesium sulfate heptahydrate, 1.0g of potassium dihydrogen phosphate and 20g of agar into 100g of freshly squeezed sugarcane juice, uniformly stirring to completely dissolve all the components, adding water to a constant volume of 1L, and sterilizing the solution at 121 ℃ and 105KPa for 20min to obtain the sugarcane juice culture medium.
Example 4
A method for promoting efficient spore production of Arctostaphylos metschlechleri comprises the following steps: activating and culturing the arthrobacter on a PDA culture medium for 7 days, beating a bacterial cake with the diameter of 5mm at the edge of a bacterial colony, inoculating the bacterial cake into a potato juice culture medium, and then putting the potato juice culture medium into a constant-temperature incubator to perform constant-temperature culture for 15 days at 28 ℃.
The potato juice culture medium contains the following substances in proportion: 50g/L of potato, 20g/L of glucose and 20g/L of agar. Cutting 50g of potato into small pieces of about 2cm × 2cm, boiling in boiling water for 30min, and filtering with gauze to obtain potato juice; adding 20g of glucose and 20g of agar into the potato juice to completely dissolve the components, adding water to a constant volume of 1L, and sterilizing the solution at 121 ℃ and 105KPa for 20min to obtain the potato juice culture medium.
Example 5
A method for promoting efficient spore production of Arctostaphylos metschlechleri comprises the following steps: activating and culturing the arthrobacter on a PDA culture medium for 7 days, beating a bacterial cake with the diameter of 5mm at the edge of a bacterial colony, inoculating the bacterial cake into a potato juice culture medium, and then putting the potato juice culture medium into a constant-temperature incubator to perform constant-temperature culture for 15 days at 28 ℃.
The potato juice culture medium contains the following substances in proportion: 100g/L of potato, 20g/L of glucose and 20g/L of agar. Cutting 100g of potato into small pieces of 2cm × 2cm, boiling in boiling water for 30min, and filtering with gauze to obtain potato juice; adding 20g of glucose and 20g of agar into the potato juice to completely dissolve the components, adding water to a constant volume of 1L, and sterilizing the solution at 121 ℃ and 105KPa for 20min to obtain the potato juice culture medium.
Example 6
A method for promoting efficient spore production of Arctostaphylos metschlechleri comprises the following steps: activating and culturing the arthrobacter on a PDA culture medium for 7 days, beating a bacterial cake with the diameter of 5mm at the edge of a bacterial colony, inoculating the bacterial cake into a potato juice culture medium, and then putting the potato juice culture medium into a constant-temperature incubator to perform constant-temperature culture for 15 days at 28 ℃.
The potato juice culture medium contains the following substances in proportion: 200g/L of potato, 20g/L of glucose and 20g/L of agar. Cutting 200g of potato into small pieces of about 2cm × 2cm, boiling in boiling water for 30min, and filtering with gauze to obtain potato juice; adding 20g of glucose and 20g of agar into the potato juice to completely dissolve the components, adding water to a constant volume of 1L, and sterilizing the solution at 121 ℃ and 105KPa for 20min to obtain the potato juice culture medium. The potato juice culture medium containing 200g/L of potatoes is a PDA culture medium.
Example 7
A method for promoting efficient spore production of Arctostaphylos metschlechleri comprises the following steps: activating and culturing the arthrobacter on a PDA culture medium for 7 days, beating a bacterial cake with the diameter of 5mm at the edge of a bacterial colony, inoculating the bacterial cake into a potato juice culture medium, and then putting the potato juice culture medium into a constant-temperature incubator to perform constant-temperature culture for 15 days at 28 ℃.
The potato juice culture medium contains the following substances in proportion: 400g/L of potato, 20g/L of glucose and 20g/L of agar. Taking 400g of potatoes, cutting into small pieces of about 2cm multiplied by 2cm, boiling in boiling water for 30min, and filtering with gauze to obtain potato juice; adding 20g of glucose and 20g of agar into the potato juice to completely dissolve the components, adding water to a constant volume of 1L, and sterilizing the solution at 121 ℃ and 105KPa for 20min to obtain the potato juice culture medium.
In order to highlight that the culture medium of the invention can promote the efficient spore production of the rhodosporidium toruloides, the applicant also carried out the following tests using other commonly used culture media:
comparative example 1
Activating and culturing the arthritic strains on a PDA culture medium for 7 days, beating a bacterial cake with the diameter of 5mm at the edge of a bacterial colony, inoculating the bacterial cake into a PSA culture medium, and then putting the PSA culture medium into a constant-temperature incubator to perform constant-temperature culture for 15 days at 28 ℃.
The PSA culture medium contains the following substances in proportion: 200g/L of potato, 20g/L of cane sugar and 20g/L of agar. Cutting 200g of potato into small pieces of about 2cm × 2cm, boiling in boiling water for 30min, and filtering with gauze to obtain potato juice; adding 20g of sucrose and 20g of agar into the potato juice to completely dissolve the components, adding water to a constant volume of 1L, and sterilizing the solution at 121 ℃ and 105KPa for 20min to obtain the PSA culture medium.
Comparative example 2
Activating and culturing the arthritic strains on a PDA culture medium for 7 days, beating a bacterial cake with the diameter of 5mm at the edge of a bacterial colony, inoculating the bacterial cake into a corn starch culture medium, and then putting the bacterial cake into a constant-temperature incubator to perform constant-temperature culture for 15 days at the temperature of 28 ℃.
The corn starch culture medium (CS, basic culture medium) contains the following substances in proportion: 3g/L of corn starch, 1.0g/L of magnesium sulfate heptahydrate, 1.0g/L of potassium dihydrogen phosphate and 20g/L of agar. Mixing the raw materials uniformly according to a ratio by a conventional method, and sterilizing at 121 ℃ and 105KPa for 20min to obtain the corn starch culture medium.
Comparative example 3
Activating and culturing the arthritic strains on a PDA (PDA) culture medium for 7d, beating a bacterial cake with the diameter of 5mm at the edge of a bacterial colony, inoculating the bacterial cake into an SNA culture medium, and then putting the bacterial cake into a constant-temperature incubator to perform constant-temperature culture for 15d at 28 ℃.
The SNA culture medium contains the following substances in proportion: 1.0g/L potassium dihydrogen phosphate, 1.0g/L potassium nitrate, 0.5g/L potassium chloride, 0.5g/L magnesium sulfate heptahydrate, 0.2g/L sucrose, 0.2g/L glucose and 20g/L agar. The SNA culture medium is obtained by uniformly mixing the raw materials according to the proportion by a conventional method and then sterilizing the mixture for 20min at 121 ℃ and 105 KPa.
After activated culture and inoculation to a new culture medium, regularly observing the spore yield of the rhodosporidium on each culture medium, wherein the observation method comprises the following steps: 1 cell of the culture medium was inoculated with 1 cell of a punch having a diameter of 5mm, and the cell was placed in 1mL of sterile water to prepare a spore suspension, and the number of spores was observed under a microscope using a hemocytometer. The spore production time and spore production of Arctosporium nodosum cultured in the above 10 examples were recorded as follows:
from the above spore production time and spore production quantity, the Arthrobacter strains on the culture mediums of examples 1-5 can produce spores on day 7, and the spore production time is earlier, wherein the culture mediums of examples 2 and 5 have the best spore production effect, and not only have the earlier spore production time and the larger spore production quantity, but also are most suitable for expanding propagation of Arthrobacter.
The growth conditions of the Arthrobacter xylinum strains on the culture media at day 7 are shown in FIGS. 1-9, and it can be seen that the higher the contents of the sugarcane juice and the potato juice in the culture media are, the better the growth vigor of the strains is, but the good growth vigor of the Arthrobacter xylinum strains does not represent that the spore production quantity is large. The strain inoculated on the corn starch culture medium does not grow, the strain inoculated on the SNA culture medium only grows at the original inoculation position without diffusion growth, and the spore yield is too small to be measured.
Claims (6)
1. A method for promoting efficient spore production of Arctosporium alternifolia is characterized by comprising the following steps: activating and culturing the arthrobacter strains on a PDA culture medium for 6-8 days, inoculating the arthrobacter strains subjected to activation culture into a sugarcane juice culture medium or a potato juice culture medium, and then putting the arthrobacter strains into a constant-temperature culture box to perform constant-temperature culture at 27-29 ℃ for 7-15 days to obtain the produced arthrobacter.
2. The method for promoting efficient spore production of the arthrobacter according to claim 1, wherein the sugarcane juice culture medium contains the following substances in proportion: 30-100g/L of sugarcane juice, 1.0g/L of magnesium sulfate heptahydrate, 1.0g/L of monopotassium phosphate and 20g/L of agar; mixing the raw materials at a certain ratio, and sterilizing at 121 deg.C and 105KPa for 20min to obtain sugarcane juice culture medium; the sugarcane juice is obtained by juicing sugarcane stems and filtering.
3. The method for promoting efficient spore production of the arthrobacter according to claim 1 or 2, wherein the sugarcane juice culture medium contains the following substances in parts by weight: 50g/L of sugarcane juice, 1.0g/L of magnesium sulfate heptahydrate, 1.0g/L of monopotassium phosphate and 20g/L of agar; mixing the raw materials at a certain ratio, and sterilizing at 121 deg.C and 105KPa for 20min to obtain sugarcane juice culture medium; the sugarcane juice is obtained by juicing sugarcane stems and filtering.
4. The method for promoting efficient spore production of the arthrobacter according to claim 1, wherein the potato juice culture medium comprises the following substances in parts by weight: 50-400g/L of potato, 20g/L of glucose and 20g/L of agar; cutting potato into small pieces, decocting in boiling water for 20-40min, and filtering with gauze to obtain potato juice; and uniformly mixing the potato juice with glucose and agar according to a ratio, and sterilizing at 121 ℃ and 105KPa for 20min to obtain the potato juice culture medium.
5. The method for promoting high-efficiency spore production of the arthrobacter according to claim 1 or 4, wherein the potato juice culture medium comprises the following substances in parts by weight: 100g/L of potato, 20g/L of glucose and 20g/L of agar; cutting potato into small pieces, decocting in boiling water for 20-40min, and filtering with gauze to obtain potato juice; and uniformly mixing the potato juice with glucose and agar according to a ratio, and sterilizing at 121 ℃ and 105KPa for 20min to obtain the potato juice culture medium.
6. The method for promoting efficient spore production of the arthrobacter according to claim 1, wherein the obtained arthrobacter producing the spore is inoculated to a new sugarcane juice culture medium or a new potato juice culture medium for further propagation, and propagation is continuously carried out for multiple generations so as to breed more arthrobacter progeny.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112725198A (en) * | 2021-02-25 | 2021-04-30 | 宁波市农业科学研究院 | Culture medium and culture method for rapid culture of Monosporium kansui |
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