CN1122368A - Cloning method of hemotopoietic stem cell, ancestor cell and megacaryocyte - Google Patents
Cloning method of hemotopoietic stem cell, ancestor cell and megacaryocyte Download PDFInfo
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- CN1122368A CN1122368A CN94113972A CN94113972A CN1122368A CN 1122368 A CN1122368 A CN 1122368A CN 94113972 A CN94113972 A CN 94113972A CN 94113972 A CN94113972 A CN 94113972A CN 1122368 A CN1122368 A CN 1122368A
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Abstract
Description
Cell was through 48 hours liquid culture | Adding PF4 (5 μ g/ milliliter) hatches | Not adding PF4 hatches |
Cell quantity increases | 1.98 doubly | 3.24 doubly |
Cell doubling time (hour) | 35 | 26 |
Cell percentage ratio G1:S:G2/M in each in period: | 48.8 45.5 5.7 | 59.4 29.1 11.5 |
Cell required time in each (hour) G1:S:G2/M: in period | 16.9 15.8 2 | 15.5 7.6 3 |
Factor concentration (/ml) | ?HPP-CFC | CFU-MK | CFU-GM | BFU-E |
Do not have | ?6±1/10 5Cell | 52±8/10 5Cell | 49±6/10 5Cell | 16±2/10 5Cell |
PF4????????????5μg | ?7±1/10 5Cell | 14±3/10 5Cell * | 28±4/10 5Cell # | 9±1/10 5Cell # |
TGF-β1???????1μg | ?7±0.8/10 5Cell | 12±2/10 5Cell * | 11±2/10 5Cell # | 4±1/10 5Cell * |
ZD?????????????5μg | ?6±0.5/10 5Cell | 78±9/10 5Cell * | 56±5/10 5Cell | 18±2/10 5Cell |
PF4+ZD | ?7±1/10 5Cell | 54±11/10 5Cell | 53±5/10 5Cell | 14±1/10 5Cell |
TGF-β1+ZD | ?5±1/10 5Cell | 52±8/10 5Cell | 46±3/10 5Cell | 13±2/10 5Cell |
Concentration (/ml) | ????CFU-MK | ????CFU-GM | ????BFU-E |
ZD???????????0 ?????????????0.1μg ?????????????1μg# ?????????????10μg ?????????????100μg | ????7±1 ????21±3# ????29±3* ????27±2* ????25±3* | ????15±2 ????17±3 ????13±2 ????18±3 ????15±2 | ????0 ????0 ????0 ????0 ????0 |
MPO??????????1μg | ????35±4 | ????18±2 | ????1±0.7 |
ZD+MPO | ????72±4# | ????21±3 | ????2±1 |
IL6??????????20ng | ????18±2 | ????20±2 | ????0 |
ZD+IL6 | ????38±3# | ????24±3 | ????2±1 |
GM-CSF??????10ng | ????22±4 | ????45±3 | ????0 |
ZD+GM-CSF | ????23±3 | ????78±3# | ????0 |
EPO??????????1U | ????19±3 | ????17±3 | ????37±3 |
ZD+EPO | ????25±3 | ????19±3 | ????68±4# |
Propylene glycol alginate sodium sulfate dosage (μ g/ time) | Megalokaryocyte number ± 10 3/ femur | Megalokaryocyte progenitor cell number * 10 3/ femur | Granulocyte progenitor cell number * 10 3/ femur | Leukocyte count * 10 9/ liter | / 100 milliliters of oxyphorase grams | Platelet count * 10 9/ liter |
????0 | ?5.3±0.3 | 1.5±0.1 | ?2.5±0.2 | ?3.2±0.3 | ?14.0±0.5 | ?890±58 |
????1 | ?6.2±0.5* | 2.5±0.2 | ?2.6±0.3 | ?2.9±0.4 | ?14.2±0.4 | ?966±78 |
????10 | ?6.9±0.7* | 3.1±0.5* | ?2.7±0.2 | ?3.2±0.6 | ?14.1±0.5 | ?1120±82* |
????50 | ?6.8±0.5* | 2.9±0.4* | ?2.6±0.3 | ?3.3±0.5 | ?14.5±0.7 | ?1050±76* |
HPP-CFC | CFU-MK | CFU-GK | BFU-E | |
The medullary cell of hatching with the factor not | 4±1/10 5Cell | 25±4/10 5Cell | 32±6/10 5Cell | 24±3/10 5Cell |
The not medullary cell of hatching with the factor+ZD washing | 5±1/10 5Cell | 32±3/10 5Cell | 34±4/10 5Cell | 28±3/10 5Cell |
The medullary cell that PF4 is hatched | 6±2/10 5Cell | 13±2/10 5Cell # | 19 ± 3/105 cell # | 14 ± 2/105 cell # |
The medullary cell that PF4 is hatched+ZD washing | 5±2/10 5Cell | 2.7±2/10 5Cell | 33±4/10 5Cell | 23±3/10 5Cell |
The medullary cell growth factor | ?HPP-CFC | CFU-MK | CFU-GK???? |
The medullary cell IL3+IL6 of hatching with the factor not | ?7±2/10 5Cell | 34±3/10 5Cell | 44±7/10 5Cell |
The medullary cell SCF of hatching with the factor not | ?14±3/10 5Cell | 14±2/10 5Cell | 18±3/10 5Cell |
The medullary cell # IL3+IL6 that PF4 is hatched | ?38±2/10 5Cell * | 56±2/10 5Cell * | 54±4/10 5Cell # |
The medullary cell # SCF that PF4 is hatched | ?46±3/10 5Cell * | 49±4/10 5Cell * | 35±1/10 5Cell |
????CD34 +Cell | CFU-GM | CFU-MK | BFU-E | |
Hinder serum again | ????1.8% | 26/2×10 5Cell | 45/2×10 5Cell | 28/2×10 5Cell |
Hinder serum+ZD again | ????25% | 32/2×10 5Cell | 91/2×10 5Cell | 35/2×10 5Cell |
Cord serum | ????17% | 22/2×10 5Cell | 41/2×10 5Cell | 19/2×10 5Cell |
Cord serum+ZD | ????23% | 29/2×10 5Cell | 78/2×10 5Cell | 41/2×10 5Cell |
SCF+IL3+IL6+GM-CSF+EPO | ????19% | 29/2×10 5Cell | 40/2×10 5Cell | 36/2×10 5Cell |
SCF+IL3+IL6+GM-CSF+EPO+ZD | ????26% | 41/2×10 5Cell | 76/2×10 5Cell | 56/2×10 5Cell |
????HPP-CFC | ????CFU-GM | ????CFU-MK | ????BFU-E | |
PF4+ hinders serum again | ????2.50 | ????1.52 | ????2.72 | ????1.34 |
PF4+ hinders serum+ZD again | ????3.61 | ????1.72 | ????3.80 | ????1.63 |
The PF4+ cord serum | ????2.43 | ????1.43 | ????2.62 | ????1.36 |
PF4+ cord serum+ZD | ????3.62 | ????1.62 | ????3.45 | ????1.72 |
?PF4+IL3+IL6 | ????1.70 | ????1.72 | ????2.15 | ????1.32 |
?PF4+IL3+IL6+ZD | ????3.10 | ????1.93 | ????3.23 | ????1.56 |
?PF4+SCF+GM-CSF | ????2.12 | ????3.12 | ????1.17 | ????1.22 |
?PF4+SCF+GM-CSF+ZD | ??3.12 | ????3.52 | ????1.64 | ????1.42 |
?PF4+SCF+EPO | ????1.68 | ????1.43 | ????1.21 | ????3.12 |
?PF4+SCF+EPO+ZD | ????2.72 | ????1.62 | ????1.72 | ????3.74 |
?PF4+SCF+bFGF | ????2.37 | ????1.72 | ????1.67 | ????2.10 |
?PF4+SCF+bFGF+ZD | ????3.64 | ????2.10 | ????2.24 | ????2.42 |
Claims (6)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN94113972A CN1064081C (en) | 1994-11-04 | 1994-11-04 | Cloning method of hemotopoietic stem cell, ancestor cell and megacaryocyte |
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Application Number | Priority Date | Filing Date | Title |
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CN94113972A CN1064081C (en) | 1994-11-04 | 1994-11-04 | Cloning method of hemotopoietic stem cell, ancestor cell and megacaryocyte |
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CN1122368A true CN1122368A (en) | 1996-05-15 |
CN1064081C CN1064081C (en) | 2001-04-04 |
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Application Number | Title | Priority Date | Filing Date |
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CN94113972A Expired - Lifetime CN1064081C (en) | 1994-11-04 | 1994-11-04 | Cloning method of hemotopoietic stem cell, ancestor cell and megacaryocyte |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1302102C (en) * | 2003-12-29 | 2007-02-28 | 中国医学科学院血液学研究所 | Method for preparing megakaryocytic preparation by amplifying macronucleus ancestral cell and mature megacaryocyte and use |
CN100572528C (en) * | 2004-09-28 | 2009-12-23 | 中国人民解放军军事医学科学院野战输血研究所 | A kind of method of amplification in vitro hematopoietic stem |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1353994B (en) * | 2001-11-19 | 2011-01-12 | 上海市儿童医院 | Method for creating human/goat hemopoietic stem cell heteroplastic transplantation model |
-
1994
- 1994-11-04 CN CN94113972A patent/CN1064081C/en not_active Expired - Lifetime
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1302102C (en) * | 2003-12-29 | 2007-02-28 | 中国医学科学院血液学研究所 | Method for preparing megakaryocytic preparation by amplifying macronucleus ancestral cell and mature megacaryocyte and use |
CN100572528C (en) * | 2004-09-28 | 2009-12-23 | 中国人民解放军军事医学科学院野战输血研究所 | A kind of method of amplification in vitro hematopoietic stem |
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CN1064081C (en) | 2001-04-04 |
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Owner name: UNION STEM CELL & GENE ENGINEERING CO.,LTD Free format text: FORMER OWNER: INST. OF HEMATOLOGY, CHINESE ACADEMY OF MEDICAL SCIENCES Effective date: 20030124 Owner name: INST. OF HEMATOLOGY, CHINESE ACADEMY OF MEDICAL S Free format text: FORMER OWNER: BEITE BIOLOGICAL TECHNOLOGY CO., LTD., SHANGHAI Effective date: 20030124 |
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Effective date of registration: 20030124 Address after: 300073 Tianjin City, Nankai District Road No. 11 building sign Hung Hom Hom Patentee after: Union Stemcell & Gene Engineering Co.,Ltd. Address before: 300020 No. 288, Nanjing Road, Heping District, Tianjin Patentee before: lNSTITUTE OF HEMATOLOGY, CHINESE ACADEMY OF MEDICAL SCIENCES & PEKING UNlON MEDICAL College Effective date of registration: 20030124 Address after: 30020 No. 288, Nanjing Road, Heping District, Tianjin Patentee after: lNSTITUTE OF HEMATOLOGY, CHINESE ACADEMY OF MEDICAL SCIENCES & PEKING UNlON MEDICAL College Address before: 200031 No. 320, Yueyang Road, Shanghai Patentee before: SHANGHAI BEITE BIOTECHNOLOGY CO.,LTD. |
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