CN112229883A - Step protection structure, detection printed electrode, preparation process and application thereof - Google Patents
Step protection structure, detection printed electrode, preparation process and application thereof Download PDFInfo
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- CN112229883A CN112229883A CN202010919465.2A CN202010919465A CN112229883A CN 112229883 A CN112229883 A CN 112229883A CN 202010919465 A CN202010919465 A CN 202010919465A CN 112229883 A CN112229883 A CN 112229883A
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- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
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Abstract
The invention provides a step protection structure, a detection printing electrode, a preparation process and application thereof, wherein the step protection structure of the vitamin electrochemical detection printing electrode comprises a silver step protection structure, a carbon step protection structure and an insulating layer step protection structure from bottom to top; the detection area is arranged at one end of the vitamin electrochemical detection printed electrode, the counter electrode layer detection area of the vitamin electrochemical detection printed electrode is provided with two counter electrodes, the main electrode layer detection area of the vitamin electrochemical detection printed electrode is provided with a main electrode, and the bottom surface of the silver step protection structure is arranged in the detection area of the vitamin electrochemical detection printed electrode and is positioned between the counter electrode and the main electrode or around the main electrode and the counter electrode. The electrode with the step protection structure can effectively prevent the printed electrode from being extruded and rubbed in the subsequent preparation process, so that the discreteness of the electrode is reduced, and the high-sensitivity detection of vitamins is realized.
Description
Technical Field
The invention belongs to the field of medical detection, and particularly relates to a step protection structure, a vitamin electrochemical detection printed electrode containing the step protection structure, and a preparation process and application thereof.
Background
Vitamins are trace organic matters necessary for the growth and metabolism of organisms, vitamin deficiency can cause vitamin deficiency, and the types and the total amount of the vitamins in organisms can be known through the inspection of the vitamins, so that the effects of disease diagnosis and prevention are achieved.
In the prior art, the traditional electrochemical detection printed electrode is difficult to be used for vitamin detection because the concentration of vitamins in blood or serum samples is low and high-sensitivity detection is required, and particularly the content normal values of B-group and D-group vitamins, vitamin K and the like in a human body are in pmol-nmol level; in the high-sensitivity detection process, the requirement on the printed electrode for detection is extremely high, the traditional electrode generally comprises a substrate, a counter electrode layer, a main electrode layer and an insulating layer, but the traditional electrode is often damaged by the electrode structures in the counter electrode layer and the main electrode layer due to extrusion and friction in the preparation process or transportation in the preparation process, so that the dispersion coefficient is high, the detection precision is influenced, the wide application of the vitamin electrochemical detection printed electrode in various levels of health mechanisms is obviously limited, and other traditional detection methods such as liquid chromatography, high performance liquid chromatography-mass spectrometry, electro/chemiluminescence and the like also have the defects of poor specificity, long detection period, high cost and the like, and are difficult to popularize and apply.
Disclosure of Invention
In view of the above, an object of the present invention is to provide a step protection structure for a printed electrode for vitamin electrochemical detection, which can effectively protect the printed electrode for vitamin electrochemical detection, prevent the electrode structure from being damaged due to extrusion and friction during the preparation process, and improve the detection accuracy.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a step protection structure of a vitamin electrochemical detection printed electrode comprises a silver step protection structure, a carbon step protection structure and an insulating layer step protection structure from bottom to top; the detection area is arranged at one end of the vitamin electrochemical detection printed electrode, the counter electrode layer detection area of the vitamin electrochemical detection printed electrode is provided with two counter electrodes, the main electrode layer detection area of the vitamin electrochemical detection printed electrode is provided with a main electrode, and the bottom surface of the silver step protection structure is arranged in the detection area of the vitamin electrochemical detection printed electrode and is positioned between the counter electrode and the main electrode or around the main electrode and the counter electrode.
Further, the thickness of the silver step protection structure is greater than that of the counter electrode layer, the bottom surface of the carbonaceous step protection structure is not smaller than the upper surface of the silver step protection structure and is consistent with the thickness of the main electrode layer, and the bottom surface of the insulating layer step protection structure is not smaller than the upper surface of the carbonaceous step protection structure and is consistent with the thickness of the insulating layer of the electrode.
Furthermore, the upper surface of the silver step protection structure is in one of a triangular shape, a strip shape, a rectangular shape, a circular shape, an annular shape, a C shape, an L shape and a U shape; the insulating layer step protection structure is composed of a single layer or multiple layers of insulating paint.
Preferably, the invention further provides a three-layer step composite structure of a vitamin electrochemical detection printed electrode, which comprises a counter electrode layer, a main electrode layer, an insulating layer and a step protection structure of the vitamin electrochemical detection printed electrode, wherein the counter electrode layer, the main electrode layer and the insulating layer are stacked from bottom to top, and the bottom surface of the step protection module is arranged between or around main electrodes of the counter electrode layer and the main electrode layer of the main electrode layer; wherein, the step protection structure comprises a silver step protection structure, a carbon step protection structure and an insulating layer step protection structure from bottom to top.
The other purpose of the invention is to provide a vitamin electrochemical detection printed electrode containing a step protection structure, and the electrode is added with the step protection structure, so that the dispersion coefficient of the vitamin electrochemical detection printed electrode can be effectively reduced.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a vitamin electrochemical detection printed electrode containing a step protection structure comprises a substrate layer, a counter electrode layer, a main electrode layer, an insulating layer, a surface coating film layer and the step protection structure from bottom to top; wherein the content of the first and second substances,
the electrode structure comprises a main electrode layer, an insulating layer, a counter electrode layer and a counter electrode, wherein a detection area and an interface area are respectively arranged at two ends of the electrode, two silver counter electrodes are transversely arranged at two ends of the detection area of the counter electrode layer, a main electrode is transversely arranged at the detection area of the main electrode layer, and a step protection structure is arranged above the insulating layer and is positioned between the main electrode and the counter electrode or around the main electrode and the counter electrode.
Further, the material of the base layer is one or two of PET and polyimide.
Furthermore, the interface area is connected with the detection area through a lead area, and the interface areas of the counter electrode layer and the main electrode layer are respectively provided with three finger-shaped interfaces; the interface area of the counter electrode layer is provided with 3 silver interfaces, and the interface area of the main electrode layer is provided with 3 carbon interfaces.
Furthermore, 3 silver leads are arranged in the lead area of the counter electrode layer, the 3 silver leads are respectively connected with the 3 silver interfaces of the counter electrode layer, and 2 silver leads at two ends are also connected with the two silver counter electrodes;
the lead area of the main electrode layer is provided with 3 carbonaceous leads, the 3 carbonaceous leads are respectively connected with the 3 carbonaceous interfaces of the main electrode layer, and the middle carbonaceous lead is also connected with the main electrode.
Further, the step protection structure comprises a silver step protection structure, a carbon step protection structure and an insulating layer step protection structure from bottom to top; wherein the content of the first and second substances,
the silver step protection structure is arranged between the main electrode and the counter electrode or arranged around the main electrode and the counter electrode, and the thickness of the silver step protection structure is larger than that of the counter electrode layer.
Furthermore, the shapes of the silver counter electrode and the main electrode comprise a circle with the diameter of 1-2 mm, a rectangle with the area of 0.2mm multiplied by 0.3 mm-2 mm multiplied by 5mm, or a square with the area of 0.2mm multiplied by 0.2 mm-2 mm multiplied by 2 mm.
Further, the shape of the silver step protection structure comprises a triangle, a strip, a rectangle, a circle, a ring, a C shape, an L shape and a U shape; the carbonaceous step protection structure is consistent with or slightly larger than the silver step protection structure, and the insulating layer step protection structure is composed of a single layer or multiple layers of insulating paint.
In view of the above, the third object of the present invention is to provide the application of the electrochemical vitamin detection printed electrode containing the step protection structure in vitamin detection, so as to realize high sensitivity detection of vitamin.
In view of the above, the fourth objective of the present invention is to provide a process for preparing a printed electrode for electrochemical detection of vitamin containing a step protection structure, which can prepare a vitamin high-sensitivity detection electrode with a low dispersion coefficient.
In order to achieve the purpose, the technical scheme of the invention is as follows:
a preparation process of a vitamin electrochemical detection printed electrode containing a step protection structure comprises the following steps:
(1) preparing two silver counter electrodes at two ends of a detection area of a substrate, preparing 3 silver leads in a lead area, and preparing 3 silver interfaces in an interface area, wherein the 3 silver leads are connected with the 3 silver interfaces one by one; the detection area is the detection end of the vitamin detection printed electrode, the interface area is the interface end of the vitamin detection printed electrode, the lead area is connected with the detection area and the interface area, and 2 silver leads are connected with the two silver counter electrodes at two ends;
(2) preparing a main electrode between the two silver counter electrodes in the detection area, and printing and preparing 3 carbonaceous leads and 3 carbonaceous interfaces above the 3 silver leads and the 3 silver interfaces;
(3) preparing a silver step protection structure at the vacant position of the detection area of the substrate, and preparing a carbonaceous step protection structure with the area not smaller than that of the silver step protection structure above the silver step protection structure;
(4) preparing insulating layers at the detection area except for a main electrode and 2 silver pair electrodes and the lead area, and printing and preparing an insulating layer step protection structure consisting of single-layer or multi-layer insulating paint on the carbonaceous step protection structure;
(5) a strip-shaped low-adhesion material having the same width as the substrate is prepared in advance, and a cover film is formed on the insulating layer.
Further, the 3 carbonaceous leads and the 3 carbonaceous interfaces respectively cover the 3 silver leads and the 3 silver interfaces in an area.
Further, the silver step protection structure is arranged between the main electrode and the counter electrode or arranged around the main electrode and the counter electrode, and the thickness of the silver step protection structure is larger than that of the counter electrode layer. Preferably, the thickness of the carbonaceous step protection structure is the same as that of the main electrode layer, and the thickness of the insulating layer step protection structure is the same as that of the insulating layer.
Further, in the step (1), a screen printing process is adopted to prepare silver paste on the substrate to obtain the silver counter electrode, the silver lead and the silver interface.
The invention has the following beneficial effects: the invention provides a step protection structure, a vitamin electrochemical detection printed electrode containing the step protection structure, and a preparation process and application thereof, wherein the vitamin electrochemical detection printed electrode containing the step protection structure is characterized in that the step protection structure which is not electrically connected with a main electrode, a counter electrode, a lead and other structures is prepared around the main electrode, the counter electrode and other structures, so that the printed electrode can be effectively prevented from being extruded and rubbed in the subsequent preparation process, the dispersion coefficient of the electrode is reduced, the integrity, consistency and accuracy of the electrode structure are further ensured, and the high-sensitivity detection of the vitamin is realized; meanwhile, the invention also designs a preparation process of the vitamin electrochemical detection printed electrode with the step protection structure, has the advantages of simple and convenient processing and low cost, and is favorable for promoting the wide application and cost control of the vitamin electrochemical detection printed electrode in medical institutions.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It is obvious that the drawings in the following description are some embodiments of the invention, and that for a person skilled in the art, other drawings can be derived from them without inventive exercise.
FIG. 1 is a schematic cross-sectional view of an embodiment of an electrochemical vitamin detection printed electrode with a step protection structure according to the present invention;
FIG. 2 is a schematic cross-sectional structure of each layer of an embodiment of an electrochemical vitamin detection printed electrode with a step protection structure according to the present invention;
FIG. 3 is an exploded view of one embodiment of an electrochemical vitamin detection printed electrode with a step protection structure according to the present invention;
FIG. 4 is a schematic diagram of step guard structures disposed on both sides of an electrode in one embodiment;
FIG. 5 is a schematic view of a step guard structure disposed around an electrode in one embodiment;
FIG. 6 is a schematic diagram of step guard structures disposed on both sides of and between electrodes in one embodiment;
FIG. 7 is a schematic view of a step guard structure disposed around and between electrodes in one embodiment;
FIG. 8 is a flowchart of an embodiment of a process for preparing an electrochemical vitamin detection printed electrode with a step protection structure according to the present invention.
Reference numerals: 1. the multilayer ceramic capacitor comprises a base layer, 2, a counter electrode layer, 3, a main electrode layer, 4, an insulating layer, 5, a carbon step protection structure, 6, an insulating layer step protection structure and 7, and a surface coating layer.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention. It is to be understood that the embodiments described are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The examples are given for the purpose of better illustration of the invention, but the invention is not limited to the examples. Therefore, those skilled in the art should make insubstantial modifications and adaptations to the embodiments of the present invention in light of the above teachings and remain within the scope of the invention.
Example 1
Referring to fig. 1, 2 and 3, in particular, the electrochemical vitamin detection printed electrode with a step protection structure in this embodiment includes, from bottom to top, a substrate layer 1, a counter electrode layer 2, a main electrode layer 3, an insulating layer 4, and a surface coating film layer 7; in this embodiment, the front end of the electrode is set as a detection region, the tail end is set as an interface region, and the middle region is set as a lead region for connecting the detection region and the interface region.
In this embodiment, the substrate layer 1 may be made of PET, polyimide, etc., and the specific dimensions may fluctuate within a certain range, the width is 5-8mm, the length is 35-50mm, and the thickness is 300-600 μm.
Furthermore, 2 silver counter electrodes are transversely arranged at two ends of a detection area of the counter electrode layer 2, 3 finger-shaped interfaces are arranged in an interface area of the counter electrode layer 2, and 3 silver leads are arranged in a lead area; the 3 silver leads are respectively connected with the 3 silver interfaces of the counter electrode layer, and the 2 silver leads at the two ends are also connected with the two silver counter electrodes; the silver counter electrode structure in the embodiment can be round (diameter phi is 0.9-1.8 mm), or rectangular (0.1mm multiplied by 0.2 mm-1.8 mm multiplied by 4.8mm, the length and width of which can be increased by 0.1mm as steps according to the requirement of irradiation detection), or square (0.1mm multiplied by 0.1 mm-1.8 mm multiplied by 1.8mm, the side length of which can be increased by 0.1mm as steps) and other structures, the areas of 2 counter electrodes are equal, the structures are consistent, and the thickness is 3-15 mu m; the widths of the 3 silver leads and the 3 silver interfaces are 0.3-1.2 mm.
Furthermore, the thickness of the main electrode layer 3 is 5-15 μm, a main electrode is transversely arranged in a detection area, the main electrode is positioned in an idle position between 2 counter electrodes, 3 carbon leads are arranged in a lead area of the main electrode layer 3, 3 carbon interfaces are also arranged in an interface area, the 3 carbon leads are respectively connected with the 3 carbon interfaces of the main electrode layer, and the carbon leads in the middle are also connected with the main electrode, preferably, the carbon surface layer of the main electrode is in a round shape (the diameter phi is 0.5-2 mm), a rectangular shape (the length and the width can be increased by 0.1mm according to the detection requirement), a square shape (the length and the width can be increased by 0.2 mm-2 mm x 2mm, the side length can be increased by 0.1mm according to the detection requirement) and the like; the width of the surface layer of the 3 carbon leads is 0.5-1.5 mm; the surface layer of the 3 carbon interfaces is of a rectangular structure (0.5mm multiplied by 0.4mm to 1.5mm multiplied by 8mm, the length and the width of the surface layer can be increased by 0.1mm as steps according to the detection requirement).
In this embodiment, 3 carbonaceous leads and 3 carbonaceous interfaces of the main electrode layer 3 need to completely cover 3 silver leads and 3 silver interfaces of the counter electrode layer 2.
The insulating layer 4 is covered on the other regions except the main electrode, the 2 silver pair electrodes and the interface region; the insulating layer comprises the following materials: one or more of acrylic resin, urethane resin, polyurethane resin, polysiloxane resin, silicone resin, epoxy resin, poly-p-xylylene resin, poly-xylylene, and the like, and the insulating layer 4 functions to electrically insulate structures such as a main electrode, a counter electrode, a lead, and the like, so as to avoid the formation of interference and errors during the detection process, which results in the failure of the detection; of course, the insulating layer 4 may be formed in a multi-layered insulating layer structure by printing an insulating varnish a plurality of times.
Furthermore, the strip-shaped material (preferably completely covering the detection area) with the same width (10-12mm) as the printed electrode for the electrochemical detection of the vitamin containing the step protection structure is covered on the insulating layer 4 to form a closed structure, so that the oxidation of the main electrode and the counter electrode is avoided, the surface damage of the electrode caused by operation in subsequent cutting, packaging, transportation and use links of the main electrode and the counter electrode can be further protected, the detection accuracy is better guaranteed, the storage environment of the electrode is improved, and the effective period of the electrode is prolonged.
Preferably, the vitamin electrochemical detection printed electrode containing the step protection structure in the embodiment further comprises the step protection structure, the step protection structure is divided into 3 layers, and the step protection structure comprises a silver step protection structure, a carbon step protection structure 5 and an insulating layer step protection structure from bottom to top; the silver step protection structure can be arranged between the main electrode and the counter electrode or around the main electrode and the counter electrode, the surface shapes can be triangular, strip-shaped, rectangular, circular, annular, C-shaped, L-shaped, U-shaped and the like, the areas of the bottom surfaces of the silver step protection structure, the carbon step protection structure 5 and the insulating layer step protection structure can be consistent, and the silver step protection structure, the carbon step protection structure and the insulating layer step protection structure are overlapped layer by layer; also can increase gradually for the superficial layer structure can cover the substructure completely, avoids exposing of silver matter step protection architecture, carbonaceous step protection architecture 5, of course, in order to improve the protection effect, can also use the insulating layer step protection architecture that multilayer insulated paint constitutes, step protection architecture in this embodiment highly is higher than main electrode and counter electrode, can effectively protect the two, avoids forming fish tail or indentation in follow-up technology and operation, the testing process, leads to influencing detection validity and precision.
Further, the thickness of the step protection structure in this embodiment is slightly higher than the thickness of the electrode layer, and finally, an insulating paint layer needs to be laid on the step protection structure, that is, the step protection structure is at least thicker than the electrode by the height of one layer of insulating paint. The dimension can be selected according to actual conditions so as to form an enough supporting and protecting structure and ensure that the structure does not form electrical connection with a main electrode, a counter electrode, a lead and the like; similarly, the carbonaceous step protection structure 5 is also an enough support protection structure and ensures that the carbonaceous step protection structure is not electrically connected with structures such as a main electrode, a counter electrode, a lead and the like, and the thickness of the carbonaceous step protection structure is the same as that of the main electrode layer 4; the thickness of the insulating layer step protection structure is the same as that of the insulating layer 5.
The specific structure of the step protection structure can be adjusted according to the actual situation, and the purpose of the step protection structure is to provide a supporting function to prevent the electrode layer from being deformed by external force. As shown in fig. 4, 5, 6, and 7, the step protection structures in fig. 4 are disposed on two sides of the electrode, and the step protection structures in fig. 5 are disposed around the electrode and have a U-shaped structure; in fig. 6, the step protection structures are arranged on two sides of the electrodes and between the electrodes; the step guard structure in fig. 7 is arranged around and between the electrodes.
Referring to fig. 8, the present invention provides an embodiment of a process for preparing a printed electrode for electrochemical detection of vitamins containing a step protection structure, which specifically comprises the following steps:
s100: preparing 2 silver counter electrodes at two ends of a detection area of a substrate, preparing 3 silver leads in a lead area, and preparing 3 silver interfaces connected with the 3 silver leads one by one in an interface area; then step S200 is executed;
in the step, a substrate prepared from PET, polyimide and other materials is selected, the specific dimension of the substrate can fluctuate within a certain range, the width is 5-8mm, the length is 35-50mm, and the thickness is 300-600 mu m; then the front end of the substrate is set as a detection area, the tail end of the substrate is set as an interface area, the middle area is set as a lead area for connecting the detection area and the interface area, and silver paste is prepared on the substrate by adopting a screen printing process: 2 silver counter electrodes are transversely arranged at two ends of the detection area, the interface area is provided with 3 finger-shaped interfaces, and the lead area is provided with 3 silver leads; 3 silver leads are respectively connected with 3 silver interfaces of the counter electrode layer, 2 silver counter electrode structures can be circular (the diameter phi is 0.9-1.8 mm), rectangular (0.1mm multiplied by 0.2 mm-1.8 mm multiplied by 4.8mm, the length and width of the silver counter electrode structures can be increased by taking 0.1mm as a step according to the detection requirement), square (0.1mm multiplied by 0.1 mm-1.8 mm multiplied by 1.8mm, the side length of the silver counter electrode structures can be increased by taking 0.1mm as a step according to the detection requirement), and the like, 8 areas of two pairs of electrodes are equal, the structures are consistent, and the thickness of the two pairs of electrodes is 3-15 mu m; the widths of the 3 silver leads and the 3 silver interfaces are 0.3-1.2 mm;
s200: preparing a main electrode among 2 silver counter electrodes in the detection area, and printing and preparing 3 carbonaceous leads and 3 carbonaceous interfaces above the 3 silver leads and the 3 silver interfaces; then, step S300 is executed;
in this embodiment, the carbon paste is prepared on the substrate, the silver lead, and the silver interface by a screen printing process: preparing a main electrode at an idle position between 2 counter electrodes, preparing 3 carbon leads and 3 carbon interfaces on the 3 silver leads and the 3 silver interfaces, wherein the 3 carbon leads are respectively connected with the 3 carbon interfaces, and the middle carbon lead is also connected with the main electrode, preferably, the carbon surface layer of the main electrode can be round (diameter phi: 0.5-2 mm), rectangular (0.2mm multiplied by 0.3 mm-2 mm multiplied by 5mm, the length and width of the main electrode can be increased by taking 0.1mm as a step for detection), square (0.2mm multiplied by 0.2 mm-2 mm multiplied by 2mm, the side length of the main electrode can be increased by taking 0.1mm as a step for detection) and other structures; the width of the surface layer of the 3 carbon leads is 0.5-1.5 mm; the surface layer of the 3 carbon interfaces is of a rectangular structure (0.5mm multiplied by 0.4mm to 1.5mm multiplied by 8mm, the length and width of the carbon interfaces can be increased by taking 0.1mm as a step according to the detection requirement), and 3 carbon leads and 3 carbon interfaces need to be completely covered by 3 silver leads and 3 silver interfaces.
S300: preparing a silver step protection structure at the vacant position of the detection area of the substrate, and preparing a carbonaceous step protection structure 5 with the area not smaller than that of the silver step protection structure above the silver step protection structure; then, step S400 is executed;
in this embodiment, a silver step protection structure having a shape such as a triangle, a strip, a rectangle, a circle, a ring, a C-shape, an L-shape, a U-shape, etc. is prepared at a vacant position of the detection region, for example, between the main electrode and the counter electrode, or around the main electrode and the counter electrode, the thickness of the silver step protection structure is slightly higher than that of the silver counter electrode, and the dimension can be selected according to actual conditions, so as to support the electrode layer.
Then, forming a carbonaceous step protection structure 5 which is consistent with or slightly larger than the upper surface area of the silver step protection structure on the silver step protection structure so as to form an enough supporting protection structure and ensure that the carbonaceous step protection structure is not electrically connected with structures such as a main electrode, an electric pair 8, a lead and the like, and the thickness of the carbonaceous step protection structure is the same as that of the main electrode;
s400: preparing insulating layers at the detection region except the main electrode, the 2 silver pair electrodes and the interface region, and printing and preparing an insulating layer step protection structure on the carbonaceous step protection structure 5; then, step S500 is executed;
in this embodiment, the other regions except the main electrode, the 2 silver pair electrodes, and the interface region are prepared by a screen printing process; the insulating layer comprises the following materials: one or more of rubber, plastic, glass, ceramic, mica and asbestos, and forming one or more layers of insulating paint on the carbonaceous step protection structure 5 to form an insulating layer step protection structure consisting of single-layer or multi-layer insulating paint;
s500: a strip-shaped low-adhesion material having the same width as the substrate is prepared in advance, and a cover film is formed on the insulating layer.
In this embodiment, a strip material (preferably, a strip material having a width equal to 10-12mm) of the electrochemical detection printed electrode for vitamin containing a step protection structure is formed in advance by using a material such as low-adhesion silica gel and a mechanical processing or laser cutting process (to completely cover the detection region), and is covered on the insulating layer in step S400 to form a closed structure.
While the present invention has been described with reference to the embodiments shown in the drawings, the present invention is not limited to the embodiments, which are illustrative and not restrictive, and it will be apparent to those skilled in the art that various changes and modifications can be made therein without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (10)
1. The step protection structure of the vitamin electrochemical detection printed electrode is characterized by comprising a silver step protection structure, a carbon step protection structure and an insulating layer step protection structure from bottom to top; the detection area is arranged at one end of the vitamin electrochemical detection printed electrode, the counter electrode layer detection area of the vitamin electrochemical detection printed electrode is provided with two counter electrodes, the main electrode layer detection area of the vitamin electrochemical detection printed electrode is provided with a main electrode, and the bottom surface of the silver step protection structure is arranged in the detection area of the vitamin electrochemical detection printed electrode and is positioned between the counter electrode and the main electrode or around the main electrode and the counter electrode.
2. The step protection structure according to claim 1, wherein the silver step protection structure has a thickness higher than that of the counter electrode layer, the carbonaceous step protection structure has a bottom surface not smaller than an upper surface of the silver step protection structure and a thickness corresponding to that of the main electrode layer, and the insulating layer step protection structure has a bottom surface not smaller than an upper surface of the carbonaceous step protection structure and a thickness corresponding to that of the insulating layer of the electrode.
3. The step protection structure of claim 2, wherein the top surface of the silver step protection structure is shaped as one of a triangle, a bar, a rectangle, a circle, a ring, a C-shape, an L-shape, and a U-shape; the insulating layer step protection structure is composed of a single layer or multiple layers of insulating paint.
4. A printed electrode for vitamin electrochemical detection containing a step protection structure, which is characterized by comprising a substrate layer, a counter electrode layer, a main electrode layer, an insulating layer, a surface coating film layer and the step protection structure according to any one of claims 1 to 3 from bottom to top; wherein the content of the first and second substances,
the two ends of the electrode are respectively provided with a detection area and an interface area, two silver counter electrodes are transversely arranged at the two ends of the detection area of the counter electrode layer, a main electrode is transversely arranged at the detection area of the main electrode layer, and the bottom surface of the step protection structure is positioned between the main electrode and the counter electrode or around the main electrode and the counter electrode.
5. The vitamin electrochemical detection printed electrode containing the step protection structure, according to claim 4, wherein the interface region is connected with the detection region through a lead region, and the interface regions of the counter electrode layer and the main electrode layer are provided with three finger interfaces; the interface area of the counter electrode layer is provided with 3 silver interfaces, and the interface area of the main electrode layer is provided with 3 carbon interfaces.
6. The vitamin electrochemical detection printed electrode with the step protection structure as claimed in claim 5, wherein the lead region of the counter electrode layer is provided with 3 silver leads, the 3 silver leads are respectively connected with the 3 silver interfaces of the counter electrode layer, and 2 silver leads at two ends are further connected with the two silver counter electrodes;
the lead area of the main electrode layer is provided with 3 carbonaceous leads, the 3 carbonaceous leads are respectively connected with the 3 carbonaceous interfaces of the main electrode layer, and the middle carbonaceous lead is also connected with the main electrode.
7. Use of the printed electrode for electrochemical detection of vitamins containing a step protection structure according to any one of claims 4 to 6 for detecting vitamins.
8. A preparation process of a vitamin electrochemical detection printed electrode containing a step protection structure is characterized by comprising the following steps:
(1) preparing two silver counter electrodes at two ends of a detection area of a substrate, preparing 3 silver leads in a lead area, and preparing 3 silver interfaces connected with the 3 silver leads one by one in an interface area; the detection area is the detection end of the vitamin detection printed electrode, the interface area is the interface end of the vitamin detection printed electrode, the lead area is connected with the detection area and the interface area, and 2 silver leads are connected with the two silver counter electrodes at two ends;
(2) preparing a main electrode between the two silver counter electrodes in the detection area, and printing and preparing 3 carbonaceous leads and 3 carbonaceous interfaces above the 3 silver leads and the 3 silver interfaces;
(3) preparing a silver step protection structure at the vacant position of the detection area of the substrate, and preparing a carbonaceous step protection structure with the area not smaller than that of the silver step protection structure above the silver step protection structure;
(4) preparing insulating layers at the detection area except for a main electrode and 2 silver pair electrodes and the lead area, and printing and preparing an insulating layer step protection structure consisting of single-layer or multi-layer insulating paint on the carbonaceous step protection structure;
(5) a strip-shaped low-adhesion material having the same width as the substrate is prepared in advance, and a cover film is formed on the insulating layer.
9. The process for preparing the vitamin electrochemical detection printed electrode containing the step protection structure as claimed in claim 8, wherein the 3 carbonaceous leads and the 3 carbonaceous interfaces are covered by 3 carbonaceous interfaces respectively.
10. The process for preparing a vitamin electrochemical detection printed electrode containing a step protection structure as claimed in claim 8, wherein the silver step protection structure is disposed between or around the main electrode and the counter electrode, and the thickness of the silver step protection structure is greater than that of the counter electrode layer.
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