CN112221384B

CN112221384B - Mixing device for pepsinogen recombinant protein and monoclonal antibody preparation thereof

Info

Publication number: CN112221384B
Application number: CN202011461603.3A
Authority: CN
Inventors: 曾宪亮; 路爱成; 宣善浪
Original assignee: Nanjing Aubrime Abm Biotechnology Co ltd
Current assignee: Nanjing Aubrime Abm Biotechnology Co ltd
Priority date: 2020-12-14
Filing date: 2020-12-14
Publication date: 2021-03-09
Anticipated expiration: 2040-12-14
Also published as: CN112221384A

Abstract

The invention belongs to the technical field of biotechnology, and discloses a mixing device for preparing pepsinogen recombinant protein and a monoclonal antibody thereof. This pepsinogen recombinant protein is with compounding device rather than monoclonal antibody preparation, including the mixing box, the pan feeding mouth has been seted up to the roof of mixing box and the closing cap is equipped with, the constant temperature jacket is installed to the outer wall of mixing box, and constant temperature jacket left wall top and right wall bottom are fixed respectively to be communicated with inlet tube and outlet pipe, the bottom fixedly connected with collar of mixing box lateral wall. This pepsinogen recombinant protein is with compounding device rather than monoclonal antibody preparation, adopt the circulating pump to provide power formation circulation compounding, utilize the design of compounding mechanism, break up the caking through the design in crushed aggregates roller and crushed aggregates hole at the in-process of material circulation to in re-spraying the compounding incasement, avoid the existence of caking after the compounding finishes, the material mixes more fully, has solved the problem of proposing in the background art.

Description

Mixing device for pepsinogen recombinant protein and monoclonal antibody preparation thereof

Technical Field

The invention relates to the technical field of biotechnology, in particular to a mixing device for preparing pepsinogen recombinant protein and a monoclonal antibody thereof.

Background

Need carry out the homogeneous mixing reaction operation to its preparation material when the preparation of pepsinogen recombinant protein and its monoclonal antibody, the stirring shaft stirring of traditional compounding device is usually adopted and is mixed, however not simply be liquid in the preparation material of pepsinogen recombinant protein and its monoclonal antibody, still there are a large amount of powdery material, after accomplishing the material feeding, because the existence of air, the phenomenon of caking can appear in the contact of powder and liquid material, especially under the difficult water-soluble condition of powder a bit, and this problem often is difficult to solve to traditional compounding device, the product that obtains in the mixing contains and does not break up the caking, reaction process is not abundant, influence the medicine quality, perhaps bring the safety problem even.

Disclosure of Invention

The invention aims to provide a mixing device for preparing pepsinogen recombinant protein and a monoclonal antibody thereof, and the mixing device for pepsinogen recombinant protein and the monoclonal antibody preparation thereof adopts a circular mixing mode to enhance material mixing and avoid agglomeration. The problems set forth in the background art are solved.

In order to achieve the purpose, the invention is realized by the following technical scheme: a mixing device for preparing pepsinogen recombinant protein and monoclonal antibody thereof, which comprises a mixing box, the top wall of the mixing box is provided with a feeding port and a sealing cover, the outer wall of the mixing box is provided with a constant temperature jacket, the top end of the left wall and the bottom end of the right wall of the constant temperature jacket are respectively and fixedly communicated with a water inlet pipe and a water outlet pipe, the bottom fixedly connected with collar of compounding case lateral wall, the upper surface of collar and the right side fixed mounting who is located the compounding case have the circulating pump, the fixed intercommunication of feed end of circulating pump has the play liquid end of inlet tube, the discharge end of circulating pump is through the feed inlet end that the three-way valve is fixed intercommunication respectively has drain pipe and discharging pipe, the play liquid end of drain pipe runs through the right wall of compounding case to the inside of compounding case, the edge of diapire is provided with the mechanism of gathering materials in the compounding case, the interior diapire of compounding case still is provided with compounding mechanism, the top that just is located compounding mechanism in the compounding case is provided with rabbling mechanism.

Preferably, the mechanism of gathering materials includes the collecting ring, has seted up the annular chamber in the collecting ring, and ten baffle that the annular was arranged of the inner wall fixedly connected with of collecting ring, ten holes of gathering materials with the annular chamber intercommunication are still seted up to the inner wall of collecting ring, and ten holes of gathering materials and ten baffle are crisscross to be arranged, and the feed liquor end of inlet tube runs through mixing box lateral wall and communicates with the annular chamber.

Preferably, the mixing mechanism comprises a mixing box fixedly arranged on the inner bottom wall of the mixing box, a liquid outlet hole is formed in the left side of the top wall of the mixing box, a positioning column is fixedly connected to the position of the inner bottom wall of the mixing box, which corresponds to the liquid outlet hole, a movable ring is movably sleeved on the positioning column, the upper surface and the lower surface of the movable ring are respectively contacted with the upper wall and the lower wall in the mixing box, four blade grooves are formed in the outer wall of the movable ring, blades are inserted in the blade grooves, the blades are movably connected with the bottom ends of the blade grooves through a return spring, one ends of the blades, which are far away from the return spring, extend out of the blade grooves and are contacted with the inner side wall of the mixing box, four crushing holes are further formed in the movable ring, two ends of the crushing holes are respectively located on the inner wall and the outer wall of the movable ring, a first bevel gear is fixedly, four maintenance poles of outer wall fixedly connected with of retaining ring, the downthehole garrulous material roller that is provided with of crushed aggregates, set up the maintenance hole that runs through its axle center on the garrulous material roller, the one end that the retaining ring was kept away from to the maintenance pole passes the outside of maintaining hole to activity ring, and the one end that the retaining ring was kept away from to the maintenance pole is middle part of fixedly connected with U-shaped pole still, the both ends and the activity ring outer wall fixed connection of U-shaped pole, one side fixedly connected with conical gear two that the crushed aggregates roller is close to the retaining ring, and conical gear two and conical gear one mesh, the surface of garrulous material roller is equipped with the grinding layer, the irregular grinding piece on grinding layer for.

Preferably, go out the axle center coincidence of liquid hole, mixing box, reference column and movable ring four, go out the liquid hole and be eccentric design based on mixing box, cooperation movable ring and blade form similar and the structure of blade pump, when the movable ring rotates, the flexible space that makes between the blade changes, impresses liquid in the crushed aggregates downthehole, utilizes the rotation of bits of broken glass roller to break up the material, follows liquid afterwards and goes out liquid hole blowout, the upper surface of reference column and the lowest in bits of broken glass hole are located same level, and the diameter of conical gear two is eight millimeters, and the bits of broken glass roller is round platform form and its less one end and keeps away from the one end of keeping the ring for it.

Preferably, rabbling mechanism includes the bracing piece, the both ends of bracing piece respectively with the mixing box in both ends fixed connection of controlling, fixed mounting has the bearing in the through-hole in bracing piece middle part, the fixed cup has the rotary rod in the bearing, the cistern has been seted up to the bottom of rotary rod, and the notch of cistern is relative with going out the liquid hole, the rotary rod lateral wall is from last to being four puddlers that the heliciform is equipped with down, seted up in the puddler and annotated the liquid chamber and communicate with the cistern, four spouts with annotating the liquid chamber intercommunication are seted up to the lower surface of puddler along puddler axial equidistance, go out liquid hole spun liquid and get into through the spout blowout in the cistern, the slope design of spout can promote the bracing piece and rotate, make spun liquid.

Preferably, the included angle formed by the axis of the nozzle and the horizontal plane is forty-five degrees, the included angle formed by the guide plate inclining towards the direction of the material collecting hole is thirty degrees, the lower surface of the guide plate is in contact with the bottom wall of the mixing box, and the aperture range of the material collecting hole is four to five millimeters.

Preferably, the aperture of the crushing hole is ten millimeters, the diameters of the top end and the bottom end of the crushing roller are five millimeters and eight millimeters respectively, the maintaining rod is a round rod, and the axis of the maintaining rod coincides with the axis of the crushing hole.

Preferably, four the bits of broken glass hole and four blade grooves are crisscross the arranging on the movable ring, the play liquid direction of drain pipe is tangent with the outer wall of movable ring, and the drain pipe blowout liquid strikes the blade and can drive the movable ring and rotate.

By adopting the technical scheme, the invention has the beneficial effects that:

1. this pepsinogen recombinant protein is with compounding device rather than monoclonal antibody preparation, adopt the circulating pump to provide power formation circulation compounding, utilize the design of compounding mechanism, break up the caking through the design in crushed aggregates roller and crushed aggregates hole at the in-process of material circulation to in re-spraying the compounding incasement, avoid the existence of caking after the compounding finishes, the material mixes more fully, has solved the problem of proposing in the background art.

2. This pepsinogen recombinant protein is with compounding device rather than monoclonal antibody preparation, based on compounding mechanism has designed rabbling mechanism, utilizes compounding mechanism spun liquid to provide power and drives the rotary rod and rotate, makes the puddler form the stirring to the material in the compounding case, and makes the powder of being smashed the caking mix with the interior material remixing of compounding case once more in the spout spouts the material, strengthens the compounding effect.

3. This pepsinogen recombinant protein is with compounding device rather than monoclonal antibody preparation, the collection of caking powder has been strengthened in the design of mechanism of gathering materials, and the baffle blocks to collect the caking and is pumped into the circulating pump through the hole of gathering materials and circulate, and the material obtains the intensive mixing when guaranteeing the compounding completion.

Drawings

Fig. 1 is a front view of the present invention.

FIG. 2 is a front cross-sectional view of the present invention.

Fig. 3 is an enlarged view of the invention at a in fig. 2.

Fig. 4 is a schematic structural view of the movable ring of the present invention.

Fig. 5 is a schematic structural diagram of the material collecting mechanism of the present invention.

In the figure: 1 mixing box, 2 feeding ports, 3 sealing covers, 4 constant-temperature jackets, 5 collecting mechanisms, 51 collecting rings, 52 annular cavities, 53 guide plates, 54 collecting holes, 6 mixing mechanisms, 61 mixing boxes, 62 liquid outlet holes, 63 positioning columns, 64 movable rings, 65 blade grooves, 66 return springs, 67 crushing holes, 68 conical gear I, 69 upright rods, 610 maintaining rings, 611 maintaining rods, 612 crushing rollers, 613 maintaining holes, 614U-shaped rods, 615 conical gear II, 616 grinding layers, 617 blades, 7 stirring mechanisms, 71 supporting rods, 72 bearings, 73 rotating rods, 74 liquid grooves, 75 stirring rods, 76 liquid injection cavities, 77 nozzles, 8 circulating pumps, 9 liquid inlet pipes, 10 three-way valves, 11 liquid outlet pipes, 12 liquid outlet pipes, 13 water inlet pipes, 14 water outlet pipes and 15 mounting rings.

Detailed Description

Referring to fig. 1-5, the present invention provides a technical solution: a mixing device for preparing pepsinogen recombinant protein and monoclonal antibody thereof comprises a mixing box 1, wherein the top wall of the mixing box 1 is provided with a feeding port 2 and a sealing cover 3, the outer wall of the mixing box 1 is provided with a constant temperature jacket 4, the top end of the left wall and the bottom end of the right wall of the constant temperature jacket 4 are respectively and fixedly communicated with a water inlet pipe 13 and a water outlet pipe 14, the bottom end of the side wall of the mixing box 1 is fixedly connected with a mounting ring 15, the upper surface of the mounting ring 15 is fixedly provided with a circulating pump 8 positioned at the right side of the mixing box 1, the feeding end of the circulating pump 8 is fixedly communicated with the liquid outlet end of a liquid inlet pipe 9, the discharging end of the circulating pump 8 is respectively and fixedly communicated with the liquid inlet ends of a liquid outlet pipe 11 and a liquid outlet pipe 12 through a three-way valve 10, the liquid outlet end of the liquid outlet pipe 11 penetrates through the right wall of the mixing box 1 to the, and a stirring mechanism 7 is arranged in the mixing box 1 and above the mixing mechanism 6.

Aggregate mechanism 5 includes collecting ring 51, annular cavity 52 has been seted up in collecting ring 51, the baffle 53 that ten annular arrangements of inner wall fixedly connected with of collecting ring 51, ten aggregate hole 54 with annular cavity 52 intercommunication have still been seted up to collecting ring 51's inner wall, ten aggregate hole 54 and ten baffle 53 are crisscross to be arranged, the inlet end of admission pipe 9 runs through mixing box 1 lateral wall and communicates with annular cavity 52, the collection of caking powder has been strengthened in the design of mechanism 5 that gathers materials, baffle 53 blocks to collect the caking and is extracted through aggregate hole 54 and go into circulating pump 8 and circulate, the material obtains intensive mixing when guaranteeing the compounding completion.

The mixing mechanism 6 comprises a mixing box 61, the mixing box 61 is fixedly arranged on the inner bottom wall of the mixing box 1, a liquid outlet hole 62 is formed on the left side of the top wall of the mixing box 61, a positioning column 63 is fixedly connected with the position of the inner bottom wall of the mixing box 61 corresponding to the liquid outlet hole 62, a movable ring 64 is movably sleeved on the positioning column 63, the upper surface and the lower surface of the movable ring 64 are respectively contacted with the upper wall and the lower wall in the mixing box 61, four blade grooves 65 are formed in the outer wall of the movable ring 64, blades 617 are inserted in the blade grooves 65 and movably connected with the bottom ends of the blade grooves 65 through a return spring 66, one end of each blade 617, which is far away from the return spring 66, extends out of the blade groove 65 and is contacted with the inner side wall of the mixing box 61, four crushing holes 67 are further formed in the movable ring 64, two ends of each crushing hole 67 are respectively positioned on the inner vertical rod wall and the outer vertical rod wall of, a maintaining ring 610 is movably sleeved on the upright rod 69, four maintaining rods 611 are fixedly connected to the outer wall of the maintaining ring 610, a crushing roller 612 is arranged in the crushing hole 67, a maintaining hole 613 penetrating through the axis of the crushing roller 612 is formed in the crushing roller 612, one end, far away from the maintaining ring 610, of the maintaining rod 611 penetrates through the maintaining hole 613 to the outer side of the movable ring 64, one end, far away from the maintaining ring 610, of the maintaining rod 611 is further fixedly connected with the middle of a U-shaped rod 614, two ends of the U-shaped rod 614 are fixedly connected with the outer wall of the movable ring 64, a conical gear II 615 is fixedly connected to one side, close to the maintaining ring 610, of the crushing roller 612, the conical gear II 615 is meshed with a conical gear I68, a grinding layer 616 is arranged on the surface of the crushing roller 612, the grinding layer 616 is an irregular grinding block embedded on the surface of the crushing roller 612, power is, the caking is broken up through the design of crushed aggregates roller 612 and crushed aggregates hole 67 at the in-process of material circulation to spout again into mixing box 1 in, avoid the existence of caking after the compounding, the material mixes more fully, has solved the problem of proposing among the background art.

Go out liquid hole 62, mixing box 1, the axle center coincidence of four of reference column 63 and activity ring 64, it is eccentric design based on mixing box 61 to go out liquid hole 62, cooperation activity ring 64 and blade 617 form similar and the structure of blade pump, when activity ring 64 rotated, the flexible space that makes between the blade changed, in smashing material hole 67 with liquid impressing, utilize the rotation of smashing material roller 612 to break up the material, follow liquid afterwards and spout through going out liquid hole 62, the upper surface of reference column 63 is located same level with the minimum of smashing material hole 67, the diameter of two conical gear 615 is eight millimeters, smashing material roller 612 is the round platform form and its less one end is its one end of keeping away from retaining ring 610.

The stirring mechanism 7 comprises a supporting rod 71, two ends of the supporting rod 71 are respectively fixedly connected with the left end and the right end in the mixing box 1, a bearing 72 is fixedly installed in a through hole in the middle of the supporting rod 71, a rotating rod 73 is fixedly sleeved in the bearing 72, a liquid tank 74 is arranged at the bottom end of the rotating rod 73, a notch of the liquid tank 74 is opposite to the liquid outlet hole 62, four stirring rods 75 are spirally arranged on the side wall of the rotating rod 73 from top to bottom, a liquid injection cavity 76 is formed in each stirring rod 75 and communicated with the liquid tank 74, four nozzles 77 communicated with the liquid injection cavity 76 are formed in the lower surface of each stirring rod 75 at equal intervals along the axial direction of the stirring rod 75, liquid sprayed out of the liquid outlet hole 62 enters the liquid tank 74 and is sprayed out through the nozzles 77, the supporting rod 71 can be pushed to rotate by the inclined design of the nozzles 77, the sprayed, utilize mixing mechanism 6 spun liquid to provide power and drive rotary rod 63 and rotate, make puddler 75 form the stirring to the material in the mixing box 1, and make when spout 77 spouts the material that the powder that is smashed the caking mix with the interior material remixing of mixing box 1 once more, strengthen the compounding effect.

The included angle formed by the axis of the nozzle 77 and the horizontal plane is forty-five degrees, the included angle formed by the guide plate 53 inclining towards the direction of the material collecting holes 54 is thirty degrees, the lower surface of the guide plate 53 is in contact with the bottom wall of the mixing box 1, and the aperture range of the material collecting holes 54 is four to five millimeters.

The diameter of the crushed material hole 67 is ten millimeters, the diameters of the top end and the bottom end of the crushed material roller 612 are five millimeters and eight millimeters respectively, the maintaining rod 611 is a round rod, and the axis of the maintaining rod is coincident with the axis of the crushed material hole 67.

The four material crushing holes 67 and the four blade grooves 65 are alternately distributed on the movable ring 64, the liquid outlet direction of the liquid outlet pipe 11 is tangent to the outer wall of the movable ring 64, and liquid sprayed out of the liquid outlet pipe 11 impacts the blades 617 to drive the movable ring 64 to rotate.

When the mixing device for preparing the pepsinogen recombinant protein and the monoclonal antibody thereof works, liquid materials are injected into a mixing box 1 through a feeding port 2, water is continuously conveyed into a constant-temperature jacket 4 to maintain the reaction temperature in the mixing box 1, a three-way valve 10 is operated to enable a circulating pump 8 to be communicated with a liquid outlet pipe 11, the circulating pump 8 is started to enable the liquid in the mixing box 1 to circulate, high-speed liquid impacts blades 617 to enable a movable ring 64 to rotate, the high-speed liquid sprayed from a liquid outlet hole 62 drives a rotating rod 73 to rotate, then powder is put into the mixing box 1, a sealing cover 3 is closed after the putting is completed, the powder enters the mixing box 1 and then is mixed with the liquid to generate caking, the caking generated after the circulation caking of the liquid materials is blocked by a guide plate 53, is sucked into the circulating pump 8 through a material collecting hole 54 and an annular cavity 52 and then is sprayed into a mixing box 61, and the caking is, the material crushing roller 612 rotates to scatter the caking, then the caking is sprayed into the liquid tank 74 and sprayed out by the nozzle 77 to be mixed continuously, the three-way valve 10 is operated to change the direction after the materials react fully, and the materials are pumped out by the circulating pump 8 and are discharged out of the mixing box 1 through the discharge pipe 12.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a pepsinogen recombinant protein and its monoclonal antibody preparation compounding device, includes mixing box (1), pan feeding mouth (2) have been seted up and be equipped with closing cap (3) to the roof of mixing box (1), constant temperature jacket (4) are installed to the outer wall of mixing box (1), and constant temperature jacket (4) left wall top and right wall bottom are fixed respectively and are communicate inlet tube (13) and outlet pipe (14), its characterized in that: the bottom end of the side wall of the mixing box (1) is fixedly connected with a mounting ring (15), a circulating pump (8) is fixedly mounted on the upper surface of the mounting ring (15) and positioned on the right side of the mixing box (1), the feeding end of the circulating pump (8) is fixedly communicated with the liquid outlet end of a liquid inlet pipe (9), the discharging end of the circulating pump (8) is respectively and fixedly communicated with the liquid inlet end of a liquid outlet pipe (11) and the liquid outlet end of a liquid outlet pipe (12) through a three-way valve (10), the liquid outlet end of the liquid outlet pipe (11) penetrates through the right wall of the mixing box (1) to the inside of the mixing box (1), the edge of the inner bottom wall of the mixing box (1) is provided with a material collecting mechanism (5), the inner bottom wall of the mixing box (1) is also provided with a mixing mechanism (6), and a stirring mechanism;

the collecting mechanism (5) comprises a collecting ring (51), an annular cavity (52) is formed in the collecting ring (51), ten guide plates (53) which are arranged in an annular mode are fixedly connected to the inner wall of the collecting ring (51), ten collecting holes (54) communicated with the annular cavity (52) are further formed in the inner wall of the collecting ring (51), the ten collecting holes (54) and the ten guide plates (53) are arranged in a staggered mode, and the liquid inlet end of the liquid inlet pipe (9) penetrates through the side wall of the mixing box (1) and is communicated with the annular cavity (52);

the mixing mechanism (6) comprises a mixing box (61), the mixing box (61) is fixedly installed on the inner bottom wall of the mixing box (1), a liquid outlet hole (62) is formed in the left side of the top wall of the mixing box (61), a positioning column (63) is fixedly connected to the position, corresponding to the liquid outlet hole (62), of the inner bottom wall of the mixing box (61), a movable ring (64) is movably sleeved on the positioning column (63), the upper surface and the lower surface of the movable ring (64) are respectively contacted with the upper wall and the lower wall in the mixing box (61), four blade grooves (65) are formed in the outer wall of the movable ring (64), blades (617) are inserted in the blade grooves (65), the blades (617) are movably connected with the bottom ends of the blade grooves (65) through a return spring (66), one ends, far away from the return spring (66), of the blades (617) extend out of the blade grooves (65) and are contacted with the inner side wall of the mixing box (61), and four material crushing holes, two ends of the material crushing hole (67) are respectively positioned on the inner wall and the outer wall of the movable ring (64), the upper surface of the positioning column (63) is fixedly connected with a first conical gear (68), the middle part of the upper surface of the positioning column (63) is also fixedly connected with a vertical rod (69), a maintaining ring (610) is movably sleeved on the vertical rod (69), the outer wall of the maintaining ring (610) is fixedly connected with four maintaining rods (611), a material crushing roller (612) is arranged in the material crushing hole (67), a maintaining hole (613) penetrating through the axis of the material crushing roller (612) is formed in the material crushing roller (612), one end, far away from the maintaining ring (610), of each maintaining rod (611) penetrates through the maintaining hole (613) to the outer side of the movable ring (64), the middle part of the U-shaped rod (614) is also fixedly connected with one end, far away from the maintaining ring (610), two ends of the U-shaped rod (614) are fixedly connected with the outer wall of the movable ring (64), one side, close to the maintaining ring (, the second conical gear (615) is meshed with the first conical gear (68), and the surface of the crushing roller (612) is provided with a grinding layer (616);

the axes of the liquid outlet hole (62), the mixing box (1), the positioning column (63) and the movable ring (64) are superposed, the upper surface of the positioning column (63) and the lowest position of the crushing hole (67) are positioned at the same horizontal height, the diameter of the conical gear II (615) is eight millimeters, the crushing roller (612) is in a circular truncated cone shape, and the smaller end of the crushing roller is the end of the crushing roller far away from the maintaining ring (610);

rabbling mechanism (7) are including bracing piece (71), the both ends of bracing piece (71) respectively with mixing box (1) interior both ends fixed connection of controlling, bracing piece (71) middle part through-hole internal fixation has bearing (72), fixed cover has connect rotary rod (73) in bearing (72), cistern (74) have been seted up to the bottom of rotary rod (73), and the notch of cistern (74) is relative with going out liquid hole (62), rotary rod (73) lateral wall is equipped with four puddlers (75) from last to being the heliciform down, it annotates liquid chamber (76) and communicates with cistern (74) to have seted up in puddler (75), the lower surface of puddler (75) is followed puddler (75) axial equidistance and is seted up four spouts (77) that communicate with annotating liquid chamber (76).

2. The mixing device for preparing the pepsinogen recombinant protein and the monoclonal antibody thereof according to the claim 1, is characterized in that: the included angle formed by the axis of the nozzle (77) and the horizontal plane is forty-five degrees, the included angle formed by the guide plate (53) inclining towards the direction of the material collecting hole (54) is thirty degrees, the lower surface of the guide plate (53) is in contact with the bottom wall of the mixing box (1), and the aperture range of the material collecting hole (54) is four-five millimeters.

3. The mixing device for preparing the pepsinogen recombinant protein and the monoclonal antibody thereof according to the claim 1, is characterized in that: the aperture of the material crushing hole (67) is ten millimeters, the diameters of the top end and the bottom end of the material crushing roller (612) are five millimeters and eight millimeters respectively, the maintaining rod (611) is a round rod, and the axis of the maintaining rod coincides with the axis of the material crushing hole (67).

4. The mixing device for preparing the pepsinogen recombinant protein and the monoclonal antibody thereof according to the claim 1, is characterized in that: the four crushing holes (67) and the four blade grooves (65) are arranged on the movable ring (64) in a staggered mode, and the liquid outlet direction of the liquid outlet pipe (11) is tangent to the outer wall of the movable ring (64).