CN112215496B - Pig ration quality assessment method, device and storage medium - Google Patents

Pig ration quality assessment method, device and storage medium Download PDF

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CN112215496B
CN112215496B CN202011094061.0A CN202011094061A CN112215496B CN 112215496 B CN112215496 B CN 112215496B CN 202011094061 A CN202011094061 A CN 202011094061A CN 112215496 B CN112215496 B CN 112215496B
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赵胜军
任莹
张慧
熊奕
姜宇翔
吴涛涛
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Abstract

The invention discloses a method, a device and a storage medium for evaluating the quality of pig ration, wherein the method for evaluating the quality of pig ration comprises the following steps: obtaining digestion energy DE of the daily ration to be measured; obtaining the digestible crude protein content DCP of the ration to be tested; obtaining the digestible acid washing fiber content dADF of the ration to be measured; obtaining the average daily feed intake ADFI of the daily ration to be measured; calculating the evaluation index PGI of the daily ration to be measured according to the formula I a The method comprises the steps of carrying out a first treatment on the surface of the Based on the evaluation index PGI a And evaluating the nutrition quality of the daily ration to be tested. The invention calculates the evaluation index PGI of the daily ration to be measured through the formula I a Based on the rating index PGI a The nutritional quality of the daily ration to be measured can be evaluated, the cost is low and the daily ration is quick, in addition, the nutritional quality of the daily ration is quantized, and the accuracy of the evaluation result is high.

Description

猪日粮品质的评定方法、装置及存储介质Method, device and storage medium for evaluating the quality of pig diets

技术领域Technical Field

本发明涉及养殖技术领域,特别涉及一种猪日粮品质的评定方法、装置及存储介质。The invention relates to the technical field of breeding, and in particular to a method, a device and a storage medium for evaluating the quality of pig diets.

背景技术Background Art

在猪饲养工业中,饲料的营养品质和利用率至关重要,通过对猪饲料营养价值进行评定,可以提高猪的生长性能和饲料利用率,从而提高猪饲养的经济效益。In the pig farming industry, the nutritional quality and utilization rate of feed are crucial. By evaluating the nutritional value of pig feed, the growth performance and feed utilization rate of pigs can be improved, thereby improving the economic benefits of pig farming.

目前我国现有的猪日粮营养品质评定主要集中于感官评定如气味、颜色、新鲜度等项目,以及对化学组分含量的测定,这样的评定方法受主观影响较大,准确率低。在传统动物营养学中,评定猪日粮营养品质最直接的方法是动物饲养试验,这一方法通常需要伴随猪漫长的生长过程,不仅耗时长、成本高,而且易受环境、疾病和猪只分组等因素影响,因此,亟需建立一种快速且准确的评定方法。At present, the existing evaluation of the nutritional quality of pig diets in my country mainly focuses on sensory evaluation such as smell, color, freshness and other items, as well as the determination of the content of chemical components. Such evaluation methods are subject to greater subjective influence and have low accuracy. In traditional animal nutrition, the most direct method to evaluate the nutritional quality of pig diets is animal feeding experiments. This method usually requires a long growth process for pigs, which is not only time-consuming and costly, but also easily affected by factors such as the environment, disease and pig grouping. Therefore, it is urgent to establish a fast and accurate evaluation method.

发明内容Summary of the invention

本发明的主要目的是提出一种猪日粮品质的评定方法、装置及存储介质,旨在快速且准确地评定生长育肥猪日粮的营养品质。The main purpose of the present invention is to provide a method, device and storage medium for evaluating the quality of pig diets, aiming to quickly and accurately evaluate the nutritional quality of growing and fattening pig diets.

为实现上述目的,本发明提出一种猪日粮品质的评定方法。所述猪日粮品质的评定方法包括以下步骤:To achieve the above object, the present invention provides a method for evaluating the quality of pig diets. The method for evaluating the quality of pig diets comprises the following steps:

获得待测日粮的消化能DE;Obtain the digestible energy DE of the diet to be tested;

获得所述待测日粮的可消化粗蛋白含量DCP;Obtaining the digestible crude protein content DCP of the diet to be tested;

获得所述待测日粮的可消化酸性洗涤纤维含量dADF;Obtaining the digestible acid detergent fiber content dADF of the test diet;

获得所述待测日粮的平均日采食量ADFI;Obtaining the average daily feed intake ADFI of the test diet;

根据公式Ⅰ计算所述待测日粮的评定指数PGIa,其中,所述公式Ⅰ为:The assessment index PGI a of the diet to be tested is calculated according to Formula I, wherein the Formula I is:

根据所述评定指数PGIa,评定所述待测日粮的营养品质。According to the evaluation index PGI a , the nutritional quality of the diet to be tested is evaluated.

可选地,根据所述评定指数PGIa,评定所述待测日粮的营养品质的步骤包括:Optionally, the step of evaluating the nutritional quality of the diet to be tested according to the evaluation index PGI a comprises:

判断所述评定指数PGIa是否不小于100,若所述评定指数PGIa不小于100,判定所述待测日粮的营养品质合格。It is determined whether the evaluation index PGI a is not less than 100. If the evaluation index PGI a is not less than 100, it is determined that the nutritional quality of the diet to be tested is qualified.

可选地,根据所述评定指数PGIa,评定所述待测日粮的营养品质的步骤包括:Optionally, the step of evaluating the nutritional quality of the diet to be tested according to the evaluation index PGI a comprises:

根据多个所述待测日粮的评定指数PGIa的大小顺序,对多个所述待测日粮的营养品质进行排序。The nutritional qualities of the multiple diets to be tested are ranked according to the order of the evaluation indexes PGI a of the multiple diets to be tested.

可选地,获得待测日粮的消化能DE的步骤包括:Optionally, the step of obtaining the digestible energy DE of the diet to be tested comprises:

获得待测日粮中粗灰分含量Ash(%DM)、粗蛋白含量CP(%DM)、粗脂肪含量EE(%DM)以及中性洗涤纤维含量NDF(%DM);Obtain the crude ash content Ash (%DM), crude protein content CP (%DM), crude fat content EE (%DM) and neutral detergent fiber content NDF (%DM) in the diet to be tested;

根据公式Ⅱ计算所述待测日粮的消化能DE,其中,所述公式Ⅱ为:DE=4168-(9.1×Ash)+(1.9×CP)+(3.9×EE)-(3.6×NDF)。The digestible energy DE of the test diet was calculated according to Formula II, wherein Formula II is: DE=4168-(9.1×Ash)+(1.9×CP)+(3.9×EE)-(3.6×NDF).

可选地,获得所述待测日粮的可消化粗蛋白含量DCP的步骤包括:Optionally, the step of obtaining the digestible crude protein content DCP of the diet to be tested comprises:

获得所述待测日粮中粗蛋白含量CP;Obtaining the crude protein content CP in the diet to be tested;

获取所述待测日粮的CP消化率;Obtaining the CP digestibility of the diet to be tested;

获取所述待测日粮的干物质含量DM;Obtaining the dry matter content DM of the diet to be tested;

根据公式Ⅲ计算所述待测日粮的可消化粗蛋白含量DCP,其中,所述公式Ⅲ为:DCP=(CP×CP消化率)/DM。The digestible crude protein content DCP of the test diet is calculated according to Formula III, wherein the Formula III is: DCP=(CP×CP digestibility)/DM.

可选地,获得所述待测日粮的可消化酸性洗涤纤维含量dADF的步骤包括:Optionally, the step of obtaining the digestible acid detergent fiber content dADF of the diet to be tested comprises:

获得所述待测日粮中酸性洗涤纤维含量ADF;Obtaining the acid detergent fiber content ADF in the diet to be tested;

获取所述待测日粮的ADF消化率;Obtaining the ADF digestibility of the diet to be tested;

获取所述待测日粮的干物质含量DM;Obtaining the dry matter content DM of the diet to be tested;

根据公式Ⅳ计算所述待测日粮的可消化粗蛋白含量dADF,其中,所述公式Ⅳ为:dADF=(ADF×ADF消化率)/DM。The digestible crude protein content dADF of the test diet is calculated according to Formula IV, wherein the Formula IV is: dADF=(ADF×ADF digestibility)/DM.

可选地,获得所述待测日粮的平均日采食量ADFI的步骤包括:Optionally, the step of obtaining the average daily feed intake ADFI of the test diet comprises:

获取待食用所述待测日粮的猪的体重G;Obtaining the body weight G of the pigs to be fed with the test diet;

当所述体重G小于8Kg时,ADFI为0.3;When the body weight G is less than 8 kg, ADFI is 0.3;

当所述体重G为8~20Kg时,ADFI为0.75;When the body weight G is 8-20 kg, ADFI is 0.75;

当所述体重G为20~35Kg时,ADFI为1.45;When the body weight G is 20-35 kg, the ADFI is 1.45;

当所述体重G为35~60Kg时,ADFI为1.9;When the body weight G is 35-60 kg, the ADFI is 1.9;

当所述体重G大于60Kg时,ADFI为2.55。When the body weight G is greater than 60 kg, the ADFI is 2.55.

此外,本发明还提出一种猪日粮品质评定装置,所述猪日粮品质评定装置包括存储器、处理器及存储在所述存储器上并可在所述处理器上运行的猪日粮品质评定程序,所述猪日粮品质评定程序配置为实现如上文所述的猪日粮品质的评定方法的步骤。In addition, the present invention also proposes a pig diet quality assessment device, which includes a memory, a processor, and a pig diet quality assessment program stored in the memory and executable on the processor, wherein the pig diet quality assessment program is configured to implement the steps of the pig diet quality assessment method as described above.

此外,本发明还提出一种存储介质,所述存储介质上存储有猪日粮品质评定程序,所述猪日粮品质评定程序被处理器执行时实现如上文所述的猪日粮品质的评定方法的步骤。In addition, the present invention also proposes a storage medium, on which a pig diet quality assessment program is stored. When the pig diet quality assessment program is executed by a processor, the steps of the pig diet quality assessment method described above are implemented.

本发明提供的技术方案中,提出了一个评定指数PGIa以及公式Ⅰ,通过公式Ⅰ即可计算出待测日粮的评定指数PGIa,基于评定指数PGIa,即可评定待测日粮的营养品质,成本低且快速,此外,对日粮的营养品质进行量化,其评定结果准确性高。In the technical solution provided by the present invention, an evaluation index PGI a and formula I are proposed. The evaluation index PGI a of the diet to be tested can be calculated by formula I. Based on the evaluation index PGI a , the nutritional quality of the diet to be tested can be evaluated with low cost and rapidity. In addition, the nutritional quality of the diet is quantified, and the evaluation result is highly accurate.

附图说明BRIEF DESCRIPTION OF THE DRAWINGS

为了更清楚地说明本发明实施例或现有技术中的技术方案,下面将对实施例或现有技术描述中所需要使用的附图作简单地介绍,显而易见地,下面描述中的附图仅仅为本发明的一些实施例,对于本领域普通技术人员来讲,在不付出创造性劳动的前提下,还可以根据这些附图获得其他相关的附图。In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.

图1为本发明实施例方案涉及的硬件运行环境的设备结构示意图;FIG1 is a schematic diagram of a device structure of a hardware operating environment involved in an embodiment of the present invention;

图2为本发明提出的猪日粮品质的评定方法的一实施例的流程示意图。FIG. 2 is a schematic flow chart of an embodiment of a method for evaluating the quality of pig diets proposed by the present invention.

本发明目的的实现、功能特点及优点将结合实施例,参照附图做进一步说明。The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings.

具体实施方式DETAILED DESCRIPTION

为使本发明实施例的目的、技术方案和优点更加清楚,下面将对本发明实施例中的技术方案进行清楚、完整地描述。显然,所描述的实施例仅仅是本发明的一部分实施例,而不是全部的实施例。To make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them.

需要说明的是,实施例中未注明具体条件者,按照常规条件或制造商建议的条件进行。所用试剂或仪器未注明生产厂商者,均为可以通过市售购买获得的常规产品。此外,各个实施例之间的技术方案可以相互结合,但是必须是以本领域普通技术人员能够实现为基础,当技术方案的结合出现相互矛盾或无法实现时应当认为这种技术方案的结合不存在,也不在本发明要求的保护范围之内。基于本发明中的实施例,本领域普通技术人员在没有作出创造性劳动前提下所获得的所有其他实施例,都属于本发明保护的范围。It should be noted that, in the embodiments, those without specifying specific conditions are carried out according to conventional conditions or conditions recommended by the manufacturer. Those without specifying the manufacturer of reagents or instruments used are conventional products that can be purchased commercially. In addition, the technical schemes between the various embodiments can be combined with each other, but must be based on the ability of ordinary technicians in the field to achieve. When the combination of the technical schemes is mutually contradictory or cannot be achieved, it should be considered that the combination of such technical schemes does not exist, and is not within the scope of protection required by the present invention. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in the field without making creative work belong to the scope of protection of the present invention.

在猪饲养工业中,饲料的营养品质和利用率至关重要,通过对猪饲料营养价值进行评定,可以提高猪的生长性能和饲料利用率,从而提高猪饲养的经济效益。In the pig farming industry, the nutritional quality and utilization rate of feed are crucial. By evaluating the nutritional value of pig feed, the growth performance and feed utilization rate of pigs can be improved, thereby improving the economic benefits of pig farming.

目前我国现有的猪日粮营养品质评定主要集中于感官评定如气味、颜色、新鲜度等项目,以及对化学组分含量的测定,这样的评定方法受主观影响较大,准确率低。在传统动物营养学中,评定猪日粮营养品质最直接的方法是动物饲养试验,这一方法通常需要伴随猪漫长的生长过程,不仅耗时长、成本高,而且易受环境、疾病和猪只分组等因素影响,因此,亟需建立一种快速且准确的评定方法。At present, the existing evaluation of the nutritional quality of pig diets in my country mainly focuses on sensory evaluation such as smell, color, freshness and other items, as well as the determination of the content of chemical components. Such evaluation methods are subject to greater subjective influence and have low accuracy. In traditional animal nutrition, the most direct method to evaluate the nutritional quality of pig diets is animal feeding experiments. This method usually requires a long growth process for pigs, which is not only time-consuming and costly, but also easily affected by factors such as the environment, disease and pig grouping. Therefore, it is urgent to establish a fast and accurate evaluation method.

鉴于此,本发明提出一种猪日粮品质评定装置,请参阅图1,所述猪日粮品质评定装置可以包括:处理器1001,例如中央处理器(Central Processing Unit,CPU),通信总线1002、用户接口1003、网络接口1004,存储器1005。其中,通信总线1002用于实现这些组件之间的连接通信。用户接口1003可以包括显示屏(Display)、输入单元比如按键,可选用户接口1003还可以包括标准的有线接口、无线接口。网络接口1004可选的可以包括标准的有线接口、无线接口(如WI-FI接口)。存储器1005可以是高速随机存取存储器(Random AccessMemory,RAM),例如磁盘存储器。存储器1005可选的还可以是独立于前述处理器1001的存储装置。In view of this, the present invention proposes a device for evaluating the quality of pig diets, please refer to Figure 1, the device for evaluating the quality of pig diets may include: a processor 1001, such as a central processing unit (CPU), a communication bus 1002, a user interface 1003, a network interface 1004, and a memory 1005. Among them, the communication bus 1002 is used to realize the connection and communication between these components. The user interface 1003 may include a display screen (Display), an input unit such as a button, and the optional user interface 1003 may also include a standard wired interface and a wireless interface. The network interface 1004 may optionally include a standard wired interface and a wireless interface (such as a WI-FI interface). The memory 1005 may be a high-speed random access memory (Random Access Memory, RAM), such as a disk storage. The memory 1005 may also be a storage device independent of the aforementioned processor 1001.

本领域技术人员可以理解,图1中示出的装置的结构并不构成对设备的限定,可以包括比图示更多或更少的部件,或者组合某些部件,或者不同的部件布置。Those skilled in the art will appreciate that the structure of the device shown in FIG. 1 does not constitute a limitation on the device, and may include more or fewer components than shown, or a combination of certain components, or a different arrangement of components.

如图1所示,作为一种存储介质的存储器1005中可以包括操作系统、网络通信模块、用户接口模块以及猪日粮品质评定程序。As shown in FIG. 1 , the memory 1005 as a storage medium may include an operating system, a network communication module, a user interface module, and a pig diet quality assessment program.

在图1所示的装置中,通过处理器1001调用存储器1005中存储的猪日粮品质评定程序,并执行以下操作:In the device shown in FIG1 , the processor 1001 calls the pig diet quality assessment program stored in the memory 1005 and performs the following operations:

获得待测日粮的消化能DE;Obtain the digestible energy DE of the diet to be tested;

获得所述待测日粮的可消化粗蛋白含量DCP;Obtaining the digestible crude protein content DCP of the diet to be tested;

获得所述待测日粮的可消化酸性洗涤纤维含量dADF;Obtaining the digestible acid detergent fiber content dADF of the test diet;

获得所述待测日粮的平均日采食量ADFI;Obtaining the average daily feed intake ADFI of the test diet;

根据公式Ⅰ计算所述待测日粮的评定指数PGIa,其中,所述公式Ⅰ为:The assessment index PGI a of the diet to be tested is calculated according to Formula I, wherein the Formula I is:

根据所述评定指数PGIa,评定所述待测日粮的营养品质。According to the evaluation index PGI a , the nutritional quality of the diet to be tested is evaluated.

进一步地,处理器1001可以调用存储器1005中存储的猪日粮品质评定程序,还执行以下操作:Furthermore, the processor 1001 may call the pig diet quality assessment program stored in the memory 1005, and further perform the following operations:

根据所述评定指数PGIa,评定所述待测日粮的营养品质的步骤包括:According to the evaluation index PGI a , the steps of evaluating the nutritional quality of the diet to be tested include:

判断所述评定指数PGIa是否不小于100,若所述评定指数PGIa不小于100,判定所述待测日粮的营养品质合格。It is determined whether the evaluation index PGI a is not less than 100. If the evaluation index PGI a is not less than 100, it is determined that the nutritional quality of the diet to be tested is qualified.

进一步地,处理器1001可以调用存储器1005中存储的猪日粮品质评定程序,还执行以下操作:Furthermore, the processor 1001 may call the pig diet quality assessment program stored in the memory 1005, and further perform the following operations:

根据所述评定指数PGIa,评定所述待测日粮的营养品质的步骤包括:According to the evaluation index PGI a , the steps of evaluating the nutritional quality of the diet to be tested include:

根据多个所述待测日粮的评定指数PGIa的大小顺序,对多个所述待测日粮的营养品质进行排序。The nutritional qualities of the multiple diets to be tested are ranked according to the order of the evaluation indexes PGI a of the multiple diets to be tested.

进一步地,处理器1001可以调用存储器1005中存储的猪日粮品质评定程序,还执行以下操作:Furthermore, the processor 1001 may call the pig diet quality assessment program stored in the memory 1005, and further perform the following operations:

获得待测日粮的消化能DE的步骤包括:The steps to obtain the digestible energy DE of the diet to be tested include:

获得待测日粮中粗灰分含量Ash(%DM)、粗蛋白含量CP(%DM)、粗脂肪含量EE(%DM)以及中性洗涤纤维含量NDF(%DM);Obtain the crude ash content Ash (%DM), crude protein content CP (%DM), crude fat content EE (%DM) and neutral detergent fiber content NDF (%DM) in the diet to be tested;

根据公式Ⅱ计算所述待测日粮的消化能DE,其中,所述公式Ⅱ为:DE=4168-(9.1×Ash)+(1.9×CP)+(3.9×EE)-(3.6×NDF);The digestible energy DE of the diet to be tested is calculated according to Formula II, wherein the Formula II is: DE=4168-(9.1×Ash)+(1.9×CP)+(3.9×EE)-(3.6×NDF);

根据所述粗蛋白含量CP以及所述酸性洗涤纤维含量ADF,计算得到可消化粗蛋白含量DCP以及可消化酸性洗涤纤维含量dADF。According to the crude protein content CP and the acid detergent fiber content ADF, the digestible crude protein content DCP and the digestible acid detergent fiber content dADF are calculated.

进一步地,处理器1001可以调用存储器1005中存储的猪日粮品质评定程序,还执行以下操作:Furthermore, the processor 1001 may call the pig diet quality assessment program stored in the memory 1005, and further perform the following operations:

获得所述待测日粮的可消化粗蛋白含量DCP的步骤包括:The steps of obtaining the digestible crude protein content DCP of the diet to be tested include:

获得所述待测日粮中粗蛋白含量CP;Obtaining the crude protein content CP in the diet to be tested;

获取所述待测日粮的CP消化率;Obtaining the CP digestibility of the diet to be tested;

获取所述待测日粮的干物质含量DM;Obtaining the dry matter content DM of the diet to be tested;

根据公式Ⅲ计算所述待测日粮的可消化粗蛋白含量DCP,其中,所述公式Ⅲ为:DCP=(CP×CP消化率)/DM。The digestible crude protein content DCP of the test diet is calculated according to Formula III, wherein the Formula III is: DCP=(CP×CP digestibility)/DM.

进一步地,处理器1001可以调用存储器1005中存储的猪日粮品质评定程序,还执行以下操作:Furthermore, the processor 1001 may call the pig diet quality assessment program stored in the memory 1005, and further perform the following operations:

获得所述待测日粮的可消化酸性洗涤纤维含量dADF的步骤包括:The steps of obtaining the digestible acid detergent fiber content dADF of the diet to be tested include:

获得所述待测日粮中酸性洗涤纤维含量ADF;Obtaining the acid detergent fiber content ADF in the diet to be tested;

获取所述待测日粮的ADF消化率;Obtaining the ADF digestibility of the diet to be tested;

获取所述待测日粮的干物质含量DM;Obtaining the dry matter content DM of the diet to be tested;

根据公式Ⅳ计算所述待测日粮的可消化粗蛋白含量dADF,其中,所述公式Ⅳ为:dADF=(ADF×ADF消化率)/DM。The digestible crude protein content dADF of the test diet is calculated according to Formula IV, wherein the Formula IV is: dADF=(ADF×ADF digestibility)/DM.

进一步地,处理器1001可以调用存储器1005中存储的猪日粮品质评定程序,还执行以下操作:Furthermore, the processor 1001 may call the pig diet quality assessment program stored in the memory 1005, and further perform the following operations:

获得所述待测日粮的平均日采食量ADFI的步骤包括:The steps of obtaining the average daily feed intake ADFI of the test diet include:

当所述体重G小于8Kg时,ADFI为0.3;When the body weight G is less than 8 kg, ADFI is 0.3;

当所述体重G为8~20Kg时,ADFI为0.75;When the body weight G is 8-20 kg, ADFI is 0.75;

当所述体重G为20~35Kg时,ADFI为1.45;When the body weight G is 20-35 kg, the ADFI is 1.45;

当所述体重G为35~60Kg时,ADFI为1.9;When the body weight G is 35-60 kg, the ADFI is 1.9;

当所述体重G大于60Kg时,ADFI为2.55。When the body weight G is greater than 60 kg, the ADFI is 2.55.

参阅图2,图2为本发明提出的猪日粮品质的评定方法的一实施例,在本实施例中,所述猪日粮品质的评定方法包括以下步骤:Refer to FIG. 2 , which is an embodiment of a method for evaluating the quality of pig diets proposed by the present invention. In this embodiment, the method for evaluating the quality of pig diets includes the following steps:

步骤S10,获得待测日粮的消化能DE。Step S10, obtaining the digestible energy DE of the diet to be tested.

消化能DE是饲料可消化养分所含的能量。消化能DE的获取方法有多种,具体地,可以使用仿生消化仪直接测到,获得过程简单、快捷;也可以建立营养模型,通过体外试验获得得到,以减少购买仿生消化仪的成本投入。Digestible energy DE is the energy contained in the digestible nutrients of feed. There are many ways to obtain digestible energy DE. Specifically, it can be directly measured using a bionic digestion instrument, which is simple and quick to obtain. It can also be obtained by establishing a nutritional model and obtaining it through in vitro experiments to reduce the cost of purchasing a bionic digestion instrument.

具体地,建立营养模型,通过体外试验获得所述待测日粮的消化能DE的步骤可以包括:Specifically, the steps of establishing a nutritional model and obtaining the digestible energy DE of the diet to be tested through in vitro experiments may include:

步骤S101,获得所述待测日粮中粗灰分含量Ash(%DM)、粗蛋白含量CP(%DM)、粗脂肪含量EE(%DM)以及中性洗涤纤维含量NDF(%DM)。Step S101, obtaining the crude ash content Ash (%DM), crude protein content CP (%DM), crude fat content EE (%DM) and neutral detergent fiber content NDF (%DM) in the test diet.

其中,粗灰分含量Ash(%DM)、粗蛋白含量CP(%DM)、粗脂肪含量EE(%DM)以及中性洗涤纤维含量NDF(%DM)可以通过如下步骤获得:Among them, crude ash content Ash (%DM), crude protein content CP (%DM), crude fat content EE (%DM) and neutral detergent fiber content NDF (%DM) can be obtained by the following steps:

S1011,建立体外“胃-小肠消化”模拟系统,利用模拟系统对日粮进行预处理。S1011, establish an in vitro "stomach-small intestine digestion" simulation system and use the simulation system to pretreat the diet.

步骤S1011具体包括:将待测日粮烘干后粉碎过100目筛。称取5g(精确至0.0001g)过筛样品,加入500mg胃蛋白酶,50ml HCl/NaCl溶液,加入0.5ml细菌生长抑制剂(质量浓度为0.5%的氯霉素溶液),放入恒温摇床中,并在40℃、120r/min条件下消化l h。然后,加入2.5ml 2mol/L NaOH溶液,调节pH到6.5~7.5,并加入20mL 0.1mol/L磷酸缓冲液使pH稳定,随后加入50mg胰蛋白酶,放入恒温摇床中,并在40℃、120r/min条件下消化l h,得到底物,备用。取6~8头体重为30~50kg的育肥猪新鲜粪样,按照每克新鲜粪样加入5ml生理盐水的比例,将新鲜粪样与生理盐水混匀过滤,制成菌源。将1g底物置于发酵瓶中,加入10mL菌源和164mL培养液,通入CO2进行排空后用橡胶塞密封,然后置于恒温振荡器中,于37~40℃下振荡培养36~50h,收集发酵产物并烘干,即得日粮残渣。Step S1011 specifically includes: drying the diet to be tested and then crushing it through a 100-mesh sieve. Weigh 5g (accurate to 0.0001g) of the sieved sample, add 500mg of pepsin, 50ml of HCl/NaCl solution, add 0.5ml of bacterial growth inhibitor (chloramphenicol solution with a mass concentration of 0.5%), put it in a constant temperature shaker, and digest it for 1h at 40°C and 120r/min. Then, add 2.5ml of 2mol/L NaOH solution, adjust the pH to 6.5-7.5, and add 20mL of 0.1mol/L phosphate buffer to stabilize the pH, then add 50mg of trypsin, put it in a constant temperature shaker, and digest it for 1h at 40°C and 120r/min to obtain a substrate for standby use. Take 6-8 fresh feces samples of fattening pigs weighing 30-50kg, add 5ml of physiological saline per gram of fresh feces sample, mix the fresh feces sample with the physiological saline and filter it to make a bacterial source. Place 1 g of substrate in a fermentation bottle, add 10 mL of bacterial source and 164 mL of culture medium, pass CO2 to evacuate, seal with a rubber stopper, then place in a constant temperature oscillator, shake and culture at 37-40°C for 36-50 hours, collect the fermentation product and dry it to obtain the dietary residue.

S1012,分别检测日粮残渣以及待测日粮中的各营养成分的含量,检测项目包括:粗灰分含量Ash、粗蛋白含量CP、粗脂肪含量EE、中性洗涤纤维含量NDF以及干物质含量DM。S1012, respectively testing the contents of various nutrients in the diet residue and the diet to be tested, the test items include: crude ash content Ash, crude protein content CP, crude fat content EE, neutral detergent fiber content NDF and dry matter content DM.

具体实施时,各营养成分含量可采用本领域的常规检测方法检测,例如,可以采用凯氏定氮法检测粗蛋白含量CP;采用油重法检测粗脂肪含量EE;采用灼烧残渣法检测粗灰分含量Ash;采用酸碱法检测中性洗涤纤维含量NDF。In specific implementation, the content of each nutrient component can be detected by conventional detection methods in the field, for example, the crude protein content CP can be detected by the Kjeldahl method; the crude fat content EE can be detected by the oil weight method; the crude ash content Ash can be detected by the ignition residue method; and the neutral detergent fiber content NDF can be detected by the acid-base method.

S1013,根据公式Ⅴ计算各营养成分的消化率,其中,公式Ⅴ为:某营养成分消化率(%)=(样品中某营养成分含量-残渣中某营养成分含量)/样品中某营养成分含量)×100。S1013, calculating the digestibility of each nutrient according to Formula V, wherein Formula V is: digestibility of a certain nutrient (%) = (content of a certain nutrient in the sample - content of a certain nutrient in the residue) / content of a certain nutrient in the sample) × 100.

S1014,根据公式Ⅵ,计算出待测日粮中各营养成分的含量(%DM),其中,公式Ⅵ为:各营养成分含量(%DM)=日粮中该营养成分的百分含量(%)×该营养成分的消化率(%)/DM(%)。S1014, calculate the content of each nutrient in the test diet (%DM) according to formula VI, wherein formula VI is: content of each nutrient (%DM) = percentage content of the nutrient in the diet (%) × digestibility of the nutrient (%)/DM (%).

以CP为例,公式Ⅵ中,CP(%DM)=(粗蛋白含量CP×CP消化率)/DM(%)。同样地,基于公式Ⅵ,计算得出粗灰分含量Ash(%DM)、粗脂肪含量EE(%DM)以及中性洗涤纤维含量NDF(%DM)。Taking CP as an example, in Formula VI, CP (% DM) = (crude protein content CP × CP digestibility) / DM (%). Similarly, based on Formula VI, the crude ash content Ash (% DM), crude fat content EE (% DM) and neutral detergent fiber content NDF (% DM) are calculated.

需要说明的是,在上述步骤S1011中,所涉及的各种溶液按照下表1配制方法制得,配制方法中所有采用的试剂均为实验室常见试剂,可在市面上购得,且只是为便于理解,才采用了诸如g、ml、L等单位,但应当理解的是,本发明提供的各溶液的制备方法不受限于上述计量单位,在实际应用时,可以根据需要等比例增大或减小试剂的使用量。It should be noted that in the above step S1011, the various solutions involved are prepared according to the preparation method in Table 1 below. All reagents used in the preparation method are common laboratory reagents that can be purchased on the market. Units such as g, ml, and L are used only for ease of understanding. However, it should be understood that the preparation methods of the various solutions provided in the present invention are not limited to the above-mentioned measurement units. In actual applications, the amount of reagents used can be proportionally increased or decreased as needed.

表1配制方法Table 1 Preparation method

步骤S102,根据公式Ⅱ计算所述待测日粮的消化能DE,其中,所述公式Ⅱ为:DE=4168-(9.1×Ash)+(1.9×CP)+(3.9×EE)-(3.6×NDF)。Step S102, calculating the digestible energy DE of the diet to be tested according to Formula II, wherein Formula II is: DE=4168-(9.1×Ash)+(1.9×CP)+(3.9×EE)-(3.6×NDF).

需要说明的是,公式Ⅱ中,DE单位为kcal/Kg,需要换算成MJ/Kg。公式中涉及的Ash、CP、EE以及NDF均是指可消化粗灰分含量Ash、可消化粗脂肪含量EE以及可消化中性洗涤纤维含量NDF,所有指标以g/kg DM表示。It should be noted that in Formula II, the unit of DE is kcal/Kg, which needs to be converted into MJ/Kg. Ash, CP, EE and NDF involved in the formula refer to the digestible crude ash content Ash, digestible crude fat content EE and digestible neutral detergent fiber content NDF, and all indicators are expressed in g/kg DM.

步骤S20,获得所述待测日粮的可消化粗蛋白含量DCP。Step S20, obtaining the digestible crude protein content DCP of the diet to be tested.

DCP为可消化粗蛋白的百分含量(%),具体实施时,可以通过如下步骤实现:DCP is the percentage of digestible crude protein (%), which can be achieved by the following steps:

步骤S201,获得所述待测日粮中粗蛋白含量CP;Step S201, obtaining the crude protein content CP in the diet to be tested;

步骤S202,获取所述待测日粮的CP消化率;Step S202, obtaining the CP digestibility of the diet to be tested;

步骤S203,获取所述待测日粮的干物质含量DM;Step S203, obtaining the dry matter content DM of the diet to be tested;

步骤S204,根据公式Ⅲ计算所述待测日粮的可消化粗蛋白含量DCP,其中,所述公式Ⅲ为:DCP=(CP×CP消化率)/DM。Step S204, calculating the digestible crude protein content DCP of the diet to be tested according to Formula III, wherein the Formula III is: DCP=(CP×CP digestibility)/DM.

步骤S30,获得所述待测日粮的可消化酸性洗涤纤维含量dADF。Step S30, obtaining the digestible acid detergent fiber content dADF of the diet to be tested.

dADF为可消化酸性洗涤纤维含量(%),具体实施时,可以通过如下步骤实现:dADF is the digestible acid detergent fiber content (%), which can be achieved by the following steps:

步骤S301,获得所述待测日粮中酸性洗涤纤维含量ADF;Step S301, obtaining the acid detergent fiber content ADF in the diet to be tested;

步骤S302,获取所述待测日粮的ADF消化率;Step S302, obtaining the ADF digestibility of the diet to be tested;

步骤S303,获取所述待测日粮的干物质含量DM;Step S303, obtaining the dry matter content DM of the diet to be tested;

步骤S304,根据公式Ⅳ计算所述待测日粮的可消化粗蛋白含量dADF,其中,所述公式Ⅳ为:dADF=(ADF×ADF消化率)/DM。Step S304, calculating the digestible crude protein content dADF of the diet to be tested according to Formula IV, wherein the Formula IV is: dADF=(ADF×ADF digestibility)/DM.

需要说明的是,步骤S301和步骤S302的获取方法与上述其他营养成分的检测方法相同,在此不做赘述。It should be noted that the acquisition method of step S301 and step S302 is the same as the detection method of other nutrients mentioned above, and will not be described in detail here.

步骤S40,获得所述待测日粮的平均日采食量ADFI。Step S40, obtaining the average daily feed intake ADFI of the diet to be tested.

平均日采食量ADFI是指实际生产过程中,一定时间内生长育肥猪平均每天食用该待测日粮的重量。平均日采食量ADFI可以通过动物试验测得,由于不需要考察动物食用日粮后的其他多种生长指数,例如料重比(F/G)、腹泻率(DR)、血液生化指标等,在只测试日粮的平均日采食量ADFI时,动物试验具有较快的检测速度。Average daily feed intake (ADFI) refers to the average daily weight of the diet consumed by growing and finishing pigs within a certain period of time during the actual production process. Average daily feed intake (ADFI) can be measured through animal experiments. Since there is no need to examine other growth indexes of animals after consuming the diet, such as feed-to-weight ratio (F/G), diarrhea rate (DR), blood biochemical indicators, etc., animal experiments have a faster detection speed when only testing the average daily feed intake (ADFI) of the diet.

进一步地,目前市面上常见的日粮已经经过了多次研究试验和数据统计,而在经过多次研究试验和数据统计后,日粮组分的平均日采食量ADFI的数值已得到了确认,本实施例对多种日粮的平均日采食量ADFI进行统计后发现,在利用评定指数PGIa评定日粮品质时,其计算公式Ⅰ中涉及的平均日采食量ADFI可以按照如下分类方式获取数值。具体地,步骤S40可以包括:Furthermore, the common diets currently on the market have undergone multiple research tests and data statistics, and after multiple research tests and data statistics, the values of the average daily feed intake ADFI of the diet components have been confirmed. After statistics of the average daily feed intake ADFI of various diets in this embodiment, it is found that when the evaluation index PGI a is used to evaluate the quality of the diet, the average daily feed intake ADFI involved in its calculation formula I can obtain values according to the following classification methods. Specifically, step S40 may include:

步骤S41、获取待食用所述待测日粮的猪的体重G;Step S41, obtaining the weight G of the pigs to be fed with the diet to be tested;

步骤S42、当所述体重G小于8Kg时,ADFI为0.3Kg/d;当所述体重G为8~20Kg时,ADFI为0.75Kg/d;当所述体重G为20~35Kg时,ADFI为1.45Kg/d;当所述体重G为35~60Kg时,ADFI为1.9Kg/d;当所述体重G大于60Kg时,ADFI为2.55Kg/d。且经多次对比试验,根据体重G分布区间获取的ADFI计算得到的评定指数PGIa与根据动物试验获取的ADFI计算得到的评定指数PGIa的相对偏差不大于10%。Step S42, when the weight G is less than 8Kg, ADFI is 0.3Kg/d; when the weight G is 8-20Kg, ADFI is 0.75Kg/d; when the weight G is 20-35Kg, ADFI is 1.45Kg/d; when the weight G is 35-60Kg, ADFI is 1.9Kg/d; when the weight G is greater than 60Kg, ADFI is 2.55Kg/d. And after multiple comparative tests, the relative deviation of the assessment index PGI a calculated based on the ADFI obtained from the weight G distribution interval and the assessment index PGI a calculated based on the ADFI obtained from the animal test is no more than 10%.

步骤S50、根据公式Ⅰ计算所述待测日粮的评定指数PGIa,其中,公式Ⅰ为:Step S50, calculating the assessment index PGI a of the diet to be tested according to Formula I, wherein Formula I is:

其中,DE为待测日粮的消化能DE(单位为MJ/Kg);DCP为可消化粗蛋白质含量(%DM);dADF为酸性洗涤纤维含量(%DM);ADFI为待测日粮的平均日采食量ADFI(单位为Kg/d)。Among them, DE is the digestible energy DE of the test diet (unit: MJ/Kg); DCP is the digestible crude protein content (%DM); dADF is the acid detergent fiber content (%DM); ADFI is the average daily feed intake ADFI of the test diet (unit: Kg/d).

步骤S60、根据所述评定指数PGIa,评定所述待测日粮的营养品质。Step S60: Evaluate the nutritional quality of the diet to be tested according to the evaluation index PGI a .

评定指数PGIa将能量、蛋白质指标、纤维成分以及日粮采食量统一起来,综合评价猪日粮的营养品质,能够更加全面客观且准确地反映猪日粮的营养品质,而且,整个评价过程不涉及长时间的动物试验,不受环境、疾病和猪只分组等因素影响,评价准确且快速。The evaluation index PGI a unifies energy, protein indicators, fiber composition and dietary feed intake to comprehensively evaluate the nutritional quality of pig diets. It can reflect the nutritional quality of pig diets more comprehensively, objectively and accurately. Moreover, the entire evaluation process does not involve long-term animal experiments and is not affected by factors such as environment, disease and pig grouping. The evaluation is accurate and fast.

具体地,评定指数PGIa越高,日粮的营养品质越高。如此,基于评定指数PGIa,即可评定待测日粮的营养品质,不仅成本低且快速,此外,由于对日粮的营养品质进行了量化,其营养品质的评定结果也更加直观,具有较高的准确性。Specifically, the higher the evaluation index PGI a is, the higher the nutritional quality of the diet is. In this way, the nutritional quality of the diet to be tested can be evaluated based on the evaluation index PGI a , which is not only low-cost and fast, but also, because the nutritional quality of the diet is quantified, the evaluation result of the nutritional quality is more intuitive and has higher accuracy.

具体实施时,步骤S60可以包括以下步骤:In specific implementation, step S60 may include the following steps:

步骤S61、判断所述评定指数PGIa是否不小于100,若所述评定指数PGIa不小于100,判定所述待测日粮的营养品质合格。Step S61, judging whether the evaluation index PGI a is not less than 100. If the evaluation index PGI a is not less than 100, judging that the nutritional quality of the diet to be tested is qualified.

本实施例以评定指数PGIa为评定指标,并设定评定标准为100,当评定指数PGIa≥100时,则可以判定待测日粮的营养品质合格,能够用于饲喂生长育肥猪;反之,当评定指数PGIa小于100时,则可以判定待测日粮的营养品质不合格,不适合用于饲喂生长育肥猪。In this embodiment, the evaluation index PGI a is used as the evaluation index, and the evaluation standard is set to 100. When the evaluation index PGI a ≥100, it can be determined that the nutritional quality of the diet to be tested is qualified and can be used to feed growing and fattening pigs; conversely, when the evaluation index PGI a is less than 100, it can be determined that the nutritional quality of the diet to be tested is unqualified and is not suitable for feeding growing and fattening pigs.

此外,步骤S60还可以通过如下步骤实现:In addition, step S60 can also be implemented by the following steps:

步骤S62、根据多个所述待测日粮的评定指数PGIa的大小顺序,对多个所述待测日粮的营养品质进行排序。Step S62: Sort the nutritional qualities of the multiple daily diets to be tested according to the order of their PGI a .

基于评定指数PGIa越大,待测日粮的营养品质越高,本实施例中,按照待测日粮评定指数PGIa的大小进行排序,判定各待测日粮的营养品质的顺序。例如,假如日粮A、B、C、D分别对应的评定指数PGIa依次增大,则说明这四种日粮的营养品质依次提升。Based on the fact that the larger the evaluation index PGI a is, the higher the nutritional quality of the diet to be tested is, in this embodiment, the diets to be tested are sorted according to the size of the evaluation index PGI a to determine the order of the nutritional quality of each diet to be tested. For example, if the evaluation index PGI a corresponding to diets A, B, C, and D increases in sequence, it means that the nutritional quality of these four diets is improved in sequence.

以下结合具体实施例对本发明的技术方案作进一步详细说明,应当理解,以下实施例仅仅用以解释本发明,并不用于限定本发明。The technical solution of the present invention is further described in detail below in conjunction with specific embodiments. It should be understood that the following embodiments are only used to explain the present invention, and are not used to limit the present invention.

对下表2中列举的四组日粮进行品质评价。这四组日粮均适宜饲喂的猪体重G为24~32Kg。The quality of the four diets listed in Table 2 below was evaluated. All four diets are suitable for feeding pigs with a body weight G of 24 to 32 kg.

表2日粮配方Table 2 Diet formula

按照下述步骤对四组日粮进行评价:Four diets were evaluated as follows:

(1)将待测日粮烘干后粉碎过100目筛。称取5g(精确至0.0001g)过筛样品,加入500mg胃蛋白酶,50ml HCl/NaCl溶液,加入0.5ml细菌生长抑制剂(质量浓度为0.5%的氯霉素溶液),放入恒温摇床中,并在40℃、120r/min条件下消化l h。然后,加入2.5ml 2mol/LNaOH溶液,调节pH到6.5~7.5,并加入20mL 0.1mol/L磷酸缓冲液使pH稳定,随后加入50mg胰蛋白酶,放入恒温摇床中,并在40℃、120r/min条件下消化l h,得到底物,备用。取6~8头体重为30~50kg的育肥猪新鲜粪样,按照每克新鲜粪样加入5ml生理盐水的比例,将新鲜粪样与生理盐水混匀过滤,制成菌源。将1g底物置于发酵瓶中,加入10mL菌源和164mL培养液,通入CO2进行排空后用橡胶塞密封,然后置于恒温振荡器中,于37~40℃下振荡培养36~50h,收集发酵产物并烘干,即得日粮残渣。(1) Dry the diet to be tested and grind it through a 100-mesh sieve. Weigh 5 g (accurate to 0.0001 g) of the sieved sample, add 500 mg of pepsin, 50 ml of HCl/NaCl solution, and 0.5 ml of bacterial growth inhibitor (chloramphenicol solution with a mass concentration of 0.5%), place it in a constant temperature shaker, and digest it for 1 h at 40°C and 120 r/min. Then, add 2.5 ml of 2 mol/L NaOH solution to adjust the pH to 6.5-7.5, and add 20 mL of 0.1 mol/L phosphate buffer to stabilize the pH. Subsequently, add 50 mg of trypsin, place it in a constant temperature shaker, and digest it for 1 h at 40°C and 120 r/min to obtain the substrate for later use. Take 6-8 fresh feces samples from fattening pigs weighing 30-50 kg, add 5 ml of physiological saline per gram of fresh feces sample, mix the fresh feces sample and physiological saline, and filter them to prepare the bacterial source. Place 1 g of substrate in a fermentation bottle, add 10 mL of bacterial source and 164 mL of culture medium, pass CO2 to evacuate, seal with a rubber stopper, then place in a constant temperature oscillator, shake and culture at 37-40°C for 36-50 hours, collect the fermentation product and dry it to obtain the dietary residue.

按照如下方法分别检测日粮残渣以及待测日粮中的各营养成分的含量,结果如表3所示:The contents of various nutrients in the diet residue and the diet to be tested were tested according to the following methods. The results are shown in Table 3:

粗灰分含量Ash:灼烧残渣法;Crude ash content Ash: Ignition residue method;

粗蛋白含量CP:凯氏定氮法;Crude protein content CP: Kjeldahl method;

粗脂肪含量EE:油重法;Crude fat content EE: oil weight method;

中性洗涤纤维含量NDF:酸碱法。Neutral detergent fiber content NDF: acid-base method.

根据公式Ⅴ计算各营养成分的消化率,其中,公式Ⅴ为:某营养成分消化率(%)=(样品中某营养成分含量-残渣中某营养成分含量)/样品中某营养成分含量)×100。结果记入表3。The digestibility of each nutrient component was calculated according to Formula V, where Formula V is: digestibility of a nutrient component (%) = (content of a nutrient component in the sample - content of a nutrient component in the residue) / content of a nutrient component in the sample) × 100. The results are recorded in Table 3.

根据公式Ⅵ,计算出待测日粮中各营养成分的含量(%DM),其中,公式Ⅵ为:各营养成分含量(%DM)=日粮中该营养成分的百分含量(%)×该营养成分的消化率(%)/DM(%)。最后,根据公式Ⅱ计算A、B组日粮的消化能DE,其中,所述公式Ⅱ为:DE=4168-(9.1×Ash)+(1.9×CP)+(3.9×EE)-(3.6×NDF),结果记入表3。同时,使用仿生消化仪检测C、D组的消化能DE。According to formula VI, the content of each nutrient in the diet to be tested (%DM) is calculated, wherein formula VI is: the content of each nutrient (%DM) = the percentage content of the nutrient in the diet (%) × the digestibility of the nutrient (%) / DM (%). Finally, the digestible energy DE of the diets of groups A and B is calculated according to formula II, wherein the formula II is: DE = 4168-(9.1×Ash)+(1.9×CP)+(3.9×EE)-(3.6×NDF), and the results are recorded in Table 3. At the same time, the digestible energy DE of groups C and D was detected using a bionic digestion instrument.

(2)查阅表3,得到各组日粮的DCP和dADF值。(2) Refer to Table 3 to obtain the DCP and dADF values of each group of diets.

(3)基于待食用三组日粮的猪体重G为24~32Kg,得知四组日粮的ADFI均为1.308Kg/d。(3) Based on the body weight G of the pigs to be fed the three diets, which was 24 to 32 kg, it was found that the ADFI of the four diets was 1.308 kg/d.

(4)根据公式以及表3记载的DE、DCP以及dADF值,分别计算得各组日粮的PGIa如表4所示。(4) According to the formula Based on the DE, DCP and dADF values recorded in Table 3, the PGI a of each diet group was calculated as shown in Table 4.

(5)评定结果:从表4记录的数据可以看出,A、B、C三组日粮的PGIa均大于100,D组PGIa小于100,也就是说,A、B、C三组日粮均适合饲养猪,D组不适合。同时,A、B、C三组日粮的PGIa的大小为:B>C>A,说明三组日粮的营养品质由高到低依次为B组、C组、A组。(5) Evaluation results: From the data recorded in Table 4, it can be seen that the PGI a of the three groups of diets A, B, and C are all greater than 100, and the PGI a of group D is less than 100. In other words, the diets of groups A, B, and C are all suitable for feeding pigs, while group D is not. At the same time, the PGI a of the three groups of diets A, B, and C is: B>C>A, indicating that the nutritional quality of the three groups of diets is from high to low, group B, group C, and group A.

表3Table 3

注:同行数值中,肩标相同表示差异不显著(P>0.05),肩标不同表示差异显著(P<0.05)。Note: In the same line, the same shoulder marks indicate no significant difference (P>0.05), while different shoulder marks indicate significant difference (P<0.05).

表4各组评价指标Table 4 Evaluation indicators of each group

PGIa PGI PBGIPBGI A组Group A 125.113125.113 0.5540.554 B组Group B 174.414174.414 0.5690.569 C组Group C 130.065130.065 0.5580.558 D组Group D 99.72999.729 0.4670.467

(一)评价方法准确性考察(I) Evaluation method accuracy

以下通过饲喂试验对经本发明评定方法评定过的日粮的饲喂效果进行获得。The feeding effect of the diet evaluated by the evaluation method of the present invention is obtained through a feeding test.

选择24头健康、平均体重28.2±4.46kg的杜×长×大三元杂交猪,随机分为4组(每组6头猪),分别为4个实验组(实验组1、实验组2、实验组3、实验组4)。其中,实验组1、实验组2、实验组3、实验组4对应采用日粮A、B、C、D进行喂养。预饲期按照试验猪自由采食量的85%~90%的量准确定量饲喂,然后向试验期过渡。预饲期5天,试验期28天。考察各组育肥猪的平均日增重(ADG)、平均日采食量(ADFI)以及料重比(F/G),结果见表5。Twenty-four healthy Duroc×Chang×Da hybrid pigs with an average weight of 28.2±4.46kg were selected and randomly divided into 4 groups (6 pigs in each group), namely 4 experimental groups (experimental group 1, experimental group 2, experimental group 3, and experimental group 4). Among them, experimental group 1, experimental group 2, experimental group 3, and experimental group 4 were fed with diets A, B, C, and D respectively. In the pre-feeding period, the pigs were fed accurately and quantitatively according to 85% to 90% of the free feed intake of the experimental pigs, and then transitioned to the experimental period. The pre-feeding period was 5 days and the experimental period was 28 days. The average daily weight gain (ADG), average daily feed intake (ADFI) and feed-to-weight ratio (F/G) of the fattening pigs in each group were investigated, and the results are shown in Table 5.

表5育肥猪的生长性能对比Table 5 Comparison of growth performance of fattening pigs

ADGADG ADFIADFI F/GF/G 实验组1Experimental Group 1 529.88529.88 1308.631308.63 2.592.59 实验组2Experimental Group 2 588.63588.63 1333.631333.63 2.412.41 实验组3Experimental Group 3 571.31571.31 1349.401349.40 2.532.53 实验组4Experimental Group 4 523.57523.57 1302.631302.63 2.492.49

从上表5数据可以看出,实验组1至3的试验猪的生长性能明显优于实验组4,且实验组1至3中,实验组2的试验猪的生长性能最佳,说明根据本发明提供的日粮评定方法作出的营养品质评定结果具有准确性。It can be seen from the data in Table 5 above that the growth performance of the experimental pigs in experimental groups 1 to 3 is significantly better than that in experimental group 4, and among experimental groups 1 to 3, the growth performance of the experimental pigs in experimental group 2 is the best, indicating that the nutritional quality assessment results made by the diet assessment method provided by the present invention are accurate.

(二)评价方法精密度考察(II) Evaluation method precision study

对比例:根据计算公式PBGI=(DE×ADFI)/K计算PBGI评价指数,基于评价指数PBGI评价A、B、C三组日粮的营养品质。其中,DE和ADFI可查阅表3得到,K为ADG/DE,单位为g/MJ,可以根据中国猪饲养标准得出,基于待食用三组日粮的猪体重G为24~32Kg,三组日粮的K值均为46.3。PBGI计算结果记入表4。Comparative example: The PBGI evaluation index was calculated according to the calculation formula PBGI = (DE × ADFI) / K, and the nutritional quality of the three diets of groups A, B, and C was evaluated based on the evaluation index PBGI. DE and ADFI can be found in Table 3, K is ADG/DE, in g/MJ, which can be obtained according to the Chinese pig feeding standards, based on the weight G of the pigs to be consumed in the three diets being 24 to 32 kg, and the K values of the three diets are all 46.3. The PBGI calculation results are recorded in Table 4.

参阅表4,可以看出,A、B、C三组日粮的PBGI指数同样呈B>C>A的顺序,也就是说,根据PBGI指数,三组日粮的营养品质由高到低依次为B组、A组、C组。然而,A组和C组的PBGI指数分别为0.554和0.558,二者的相对偏差值为0.72%,而B组和C组的PGIa指数分别为125.113和130.065,二者的相对偏差值为3.9%,相较于PBGI指数,PGIa指数结果相差更大,区分度更好,实际应用时,评价精密度更好,不容易因外界因素干扰导致的随机误差影响到评定结果。Referring to Table 4, it can be seen that the PBGI index of the three groups of diets A, B, and C also presents the order of B>C>A, that is, according to the PBGI index, the nutritional quality of the three groups of diets from high to low is group B, group A, and group C. However, the PBGI index of group A and group C is 0.554 and 0.558, respectively, and the relative deviation value between the two is 0.72%, while the PGI a index of group B and group C is 125.113 and 130.065, respectively, and the relative deviation value between the two is 3.9%. Compared with the PBGI index, the PGI a index results are more different and have better discrimination. In practical application, the evaluation precision is better, and it is not easy for random errors caused by external factors to affect the evaluation results.

以上仅为本发明的优选实施例,并非因此限制本发明的专利范围,对于本领域的技术人员来说,本发明可以有各种更改和变化。凡在本发明的精神和原则之内,所作的任何修改、等同替换、改进等,均应包括在本发明的专利保护范围内。The above are only preferred embodiments of the present invention, and do not limit the patent scope of the present invention. For those skilled in the art, the present invention may have various changes and modifications. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the patent protection scope of the present invention.

Claims (7)

1.一种猪日粮品质的评定方法,其特征在于,包括以下步骤:1. A method for evaluating the quality of pig diet, which is characterized in that it includes the following steps: 获得待测日粮的消化能DE;Obtain the digestible energy DE of the diet to be tested; 获得所述待测日粮的可消化粗蛋白含量DCP;Obtain the digestible crude protein content DCP of the diet to be tested; 获得所述待测日粮的可消化酸性洗涤纤维含量dADF;Obtain the digestible acid detergent fiber content dADF of the diet to be tested; 获得所述待测日粮的平均日采食量ADFI;Obtain the average daily feed intake ADFI of the diet to be tested; 根据公式Ⅰ计算所述待测日粮的评定指数PGIa,其中,所述公式Ⅰ为:Calculate the evaluation index PGI a of the diet to be tested according to Formula I, where the Formula I is: ; 根据所述评定指数PGIa,评定所述待测日粮的营养品质;Evaluate the nutritional quality of the diet to be tested according to the evaluation index PGI a ; 其中,获得所述待测日粮的可消化粗蛋白含量DCP的步骤包括:Wherein, the steps of obtaining the digestible crude protein content DCP of the diet to be tested include: 获得所述待测日粮中粗蛋白含量CP;Obtain the crude protein content CP in the diet to be tested; 获取所述待测日粮的CP消化率;Obtain the CP digestibility of the diet to be tested; 获取所述待测日粮的干物质含量DM;Obtain the dry matter content DM of the diet to be tested; 根据公式Ⅲ计算所述待测日粮的可消化粗蛋白含量DCP,其中,所述公式Ⅲ为:DCP=(CP×CP消化率)/DM;Calculate the digestible crude protein content DCP of the diet to be tested according to formula III, where the formula III is: DCP=(CP×CP digestibility)/DM; 获得所述待测日粮的可消化酸性洗涤纤维含量dADF的步骤包括:The steps of obtaining the digestible acid detergent fiber content dADF of the diet to be tested include: 获得所述待测日粮中酸性洗涤纤维含量ADF;Obtain the acid detergent fiber content ADF in the diet to be tested; 获取所述待测日粮的ADF消化率;Obtain the ADF digestibility of the diet to be tested; 获取所述待测日粮的干物质含量DM;Obtain the dry matter content DM of the diet to be tested; 根据公式Ⅳ计算所述待测日粮的可消化粗蛋白含量dADF,其中,所述公式Ⅳ为:dADF=(ADF×ADF消化率)/DM。Calculate the digestible crude protein content dADF of the diet to be tested according to formula IV, where the formula IV is: dADF=(ADF×ADF digestibility)/DM. 2.如权利要求1所述的猪日粮品质的评定方法,其特征在于,根据所述评定指数PGIa,评定所述待测日粮的营养品质的步骤包括:2. The method for evaluating the quality of pig diets according to claim 1, wherein the step of evaluating the nutritional quality of the diet to be tested according to the evaluation index PGI a includes: 判断所述评定指数PGIa是否不小于100,若所述评定指数PGIa不小于100,判定所述待测日粮的营养品质合格。Determine whether the evaluation index PGI a is not less than 100. If the evaluation index PGI a is not less than 100, it is judged that the nutritional quality of the diet to be tested is qualified. 3.如权利要求1所述的猪日粮品质的评定方法,其特征在于,根据所述评定指数PGIa,评定所述待测日粮的营养品质的步骤包括:3. The method for evaluating the quality of pig diets according to claim 1, wherein the step of evaluating the nutritional quality of the diet to be tested according to the evaluation index PGI a includes: 根据多个所述待测日粮的评定指数PGIa的大小顺序,对多个所述待测日粮的营养品质进行排序。According to the order of the evaluation index PGI a of the multiple diets to be tested, the nutritional quality of the multiple diets to be tested is sorted. 4.如权利要求1所述的猪日粮品质的评定方法,其特征在于,获得待测日粮的消化能DE的步骤包括:4. The method for evaluating the quality of pig diets according to claim 1, wherein the step of obtaining the digestible energy DE of the diet to be tested includes: 获得待测日粮中粗灰分含量Ash(%DM)、粗蛋白含量CP(%DM)、粗脂肪含量EE(%DM)以及中性洗涤纤维含量NDF(%DM);Obtain the crude ash content Ash (%DM), crude protein content CP (%DM), crude fat content EE (%DM) and neutral detergent fiber content NDF (%DM) in the diet to be tested; 根据公式Ⅱ计算所述待测日粮的消化能DE,其中,所述公式Ⅱ为:DE = 4168-(9.1 ×Ash)+(1.9 × CP)+(3.9 × EE)-(3.6 × NDF)。Calculate the digestible energy DE of the diet to be tested according to formula II, where the formula II is: DE = 4168-(9.1 ×Ash)+(1.9 × CP)+(3.9 × EE)-(3.6 × NDF) . 5.如权利要求1所述的猪日粮品质的评定方法,其特征在于,获得所述待测日粮的平均日采食量ADFI的步骤包括:5. The method for evaluating the quality of pig diets according to claim 1, wherein the step of obtaining the average daily feed intake ADFI of the diet to be tested includes: 获取待食用所述待测日粮的猪的体重G;Obtain the body weight G of the pigs to be fed the diet to be tested; 当所述体重G小于8Kg时,ADFI为0.3;When the weight G is less than 8Kg, ADFI is 0.3; 当所述体重G为8~20Kg时,ADFI为0.75;When the weight G is 8~20Kg, ADFI is 0.75; 当所述体重G为20~35Kg时,ADFI为1.45;When the weight G is 20~35Kg, ADFI is 1.45; 当所述体重G为35~60Kg时,ADFI为1.9;When the body weight G is 35~60Kg, ADFI is 1.9; 当所述体重G大于60Kg时,ADFI为2.55。When the body weight G is greater than 60Kg, the ADFI is 2.55. 6.一种猪日粮品质评定装置,其特征在于,所述猪日粮品质评定装置包括存储器、处理器及存储在所述存储器上并可在所述处理器上运行的猪日粮品质评定程序,所述猪日粮品质评定程序配置为实现如权利要求1至5中任一项所述的猪日粮品质的评定方法的步骤。6. A pig diet quality assessment device, characterized in that the pig diet quality assessment device includes a memory, a processor, and a pig diet quality assessment program that is stored in the memory and can be run on the processor. , the pig diet quality assessment program is configured to implement the steps of the pig diet quality assessment method according to any one of claims 1 to 5. 7.一种存储介质,其特征在于,所述存储介质上存储有猪日粮品质评定程序,所述猪日粮品质评定程序被处理器执行时实现如权利要求1至5中任一项所述的猪日粮品质的评定方法的步骤。7. A storage medium, characterized in that a pig diet quality assessment program is stored on the storage medium. When the pig diet quality assessment program is executed by a processor, the pig diet quality assessment program implements the method according to any one of claims 1 to 5. The steps of the described method for evaluating the quality of pig diets.
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