CN112210575A - Process for preparing xylose by using tea leaves - Google Patents
Process for preparing xylose by using tea leaves Download PDFInfo
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- CN112210575A CN112210575A CN202011224176.7A CN202011224176A CN112210575A CN 112210575 A CN112210575 A CN 112210575A CN 202011224176 A CN202011224176 A CN 202011224176A CN 112210575 A CN112210575 A CN 112210575A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
Abstract
The invention discloses a process for preparing xylose by using tea leaves, belonging to the technical field of xylose preparation. The process comprises (1) spraying water to tea leaves to maintain humidity, and performing microwave pretreatment; (2) adding the tea leaves pretreated by microwave into a reaction kettle, performing steam penetration, and performing mechanical ball milling to obtain ball milling slurry; (3) adding the ball-milling slurry and pure water into a reaction kettle, adjusting the pH value of the solution to 5-6, controlling the temperature to be 50-60 ℃, and adding the composite hemicellulase for 2-4 hours; (4) after the reaction is finished, the xylose is prepared by plate-type filtration, ion exchange, decoloration, concentration, crystallization and drying. The method takes the tea leaves as the raw material to prepare the xylose, changes waste into valuable, realizes the recycling of the tea leaves, reduces the environmental pollution and saves resources; the preparation process is simple, the conditions are mild, the yield and the purity of the xylose are high, and the method is stable and can be used for industrial production.
Description
Technical Field
The invention belongs to the technical field of xylose preparation, and particularly relates to a process for preparing xylose by using tea leaves.
Background
Xylose is a kind of non-caloric sugar and is a variety with better physiological activity in all edible sugars. It has no increase in blood sugar level and has effect of increasing Bifidobacterium, and has advantages over other sugar alcohols. In addition, it also has effects of reducing free fatty acid in vivo, regulating lipid metabolism, controlling body weight, and preventing obesity. It is used not only for preparing xylitol and feed yeast, but also widely used in chemical, medicine, fuel and other industrial departments. At the present stage, most of xylose is prepared by using corncobs or hemicellulose separated in the papermaking industry as a raw material, and when the corncobs are used as the raw material, the adopted processes are strong acid and alkali processes, so that the pollution is very serious, and the energy consumption is relatively high; and when the hemicellulose separated from the papermaking industry is used as a raw material, the papermaking industry is also a heavy pollution industry, so the development prospect of the preparation process is not optimistic.
Nowadays, people are more and more deeply aware that tea is beneficial to human health, and develop and produce various products. Due to the opening of a large number of tea beverage plants, tea polyphenol plants and other tea deep processing plants, a large amount of tea residues are generated, the emission of the waste tea residues pollutes the environment, and the requirements of the modern economic department on continuous development are not met. Most of the tea leaves are incinerated and become an environmental pollution source, so the tea leaves are recycled, and the application prospect of changing waste into valuable is good. The method has the potential of further application for the cellulose content of the tea leaves being 11.64%, the hemicellulose content being 35.51% and the lignin content being 11.03%.
Disclosure of Invention
Aiming at the problems in the prior art, the technical problem to be solved by the invention is to provide the process for preparing the xylose by using the tea leaves, the process takes the tea leaves as the raw material to prepare the xylose, changes waste into valuable, realizes the recycling of the tea leaves, reduces the environmental pollution and saves resources.
In order to solve the problems, the technical scheme adopted by the invention is as follows:
a process for preparing xylose by using tea leaves comprises the following steps;
(1) spraying water to the tea leaves to keep humidity, and then carrying out microwave pretreatment;
(2) adding the tea leaves pretreated by the microwave into a reaction kettle for steam penetration; obtaining ball-milling slurry by adopting a mechanical ball-milling mode after the steam penetration is finished;
(3) adding the ball-milling slurry and pure water into a reaction kettle, adjusting the pH value of the solution to 5-6, controlling the temperature to be 50-60 ℃, and adding the composite hemicellulase for 2-4 hours;
(4) after the reaction is finished, the xylose is prepared by plate-type filtration, ion exchange, decoloration, concentration, crystallization and drying.
The process for preparing xylose by using the tea leaves has the humidity of 25-35%.
According to the process for preparing xylose by using tea leaves, the microwave pretreatment temperature is 50-60 ℃, the microwave pretreatment time is 20-30 min, and the microwave power is 200-300W.
According to the process for preparing xylose by using tea leaves, the microwave pretreatment temperature is 56 ℃, the time is 25min, and the microwave power is 230W.
According to the process for preparing xylose by using tea leaves, the steam penetrating through the tea leaves is at the temperature of 100-150 ℃, the pressure of 1-2 Pa and the time of 1-10 min.
According to the process for preparing xylose by using tea leaves, the steam temperature for penetrating steam is 120 ℃, the pressure is 1.3Pa, and the time is 2 min.
According to the process for preparing xylose by using tea leaves, the mass ratio of the tea leaves to pure water is 0.3: 1-0.4: 1; and adding citric acid to adjust the pH value of the solution to 5-6.
According to the process for preparing xylose by using the tea leaves, the mass ratio of the tea leaves to pure water is 0.35: 1; citric acid was added to adjust the pH of the solution to 5.8.
According to the process for preparing xylose by using the tea leaves, the using amount of the composite hemicellulase is 3% -5% of the mass of the tea leaves.
According to the process for preparing xylose by using tea leaves, in the mechanical ball milling process, a mode of mixing large balls and small balls is adopted, the diameter of the large balls is 10mm, the diameter of the small balls is 5mm, the ball milling rotation speed is 300-500 rpm, the ball milling time is 1-5 h, and the ball-to-material ratio is 10: 1-20: 1, wherein the ball-to-material ratio refers to the mass ratio of the grinding balls to the ball milling liquid.
Has the advantages that: compared with the prior art, the invention has the advantages that:
the method takes the tea leaves as the raw material to prepare the xylose, changes waste into valuable, realizes the recycling of the tea leaves, reduces the environmental pollution and saves resources; the preparation process is simple, the conditions are mild, the yield and the purity of the xylose are high, and the method is stable and can be used for industrial production.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with examples are described in detail below.
Example 1
A process for preparing xylose by using tea leaves comprises the following steps;
(1) spraying water to 35g of tea leaves, keeping the humidity at 30%, and then performing microwave pretreatment for 25min at 56 ℃; the microwave power is 230W;
(2) adding the tea leaves pretreated by the microwave into a reaction kettle for steam penetration; in the steam penetration operation, the steam temperature is 120 ℃, the pressure is 1.3Pa, and the time is 2 min; obtaining ball-milling slurry by adopting a mechanical ball-milling mode after the steam penetration is finished, wherein in the mechanical ball-milling process, a mode of mixing large balls and small balls is adopted, the diameter of the large balls is 10mm, the diameter of the small balls is 5mm, the ball-milling rotating speed is 300rpm, the ball-milling time is 2h, and the ball-to-material ratio is 10: 1;
(3) adding ball grinding materials and 100g of pure water into a reaction kettle; then adding citric acid to adjust the pH value of the solution to 5.8, controlling the temperature to be 55 ℃, adding 1.05g of composite hemicellulase, and reacting for 3 hours;
(4) and after the reaction is finished, plate-type filtration, ion exchange, decoloration, concentration, crystallization and drying are carried out to obtain 29.8g of xylose crystals.
Example 2
A process for preparing xylose by using tea leaves comprises the following steps;
(1) spraying water to 40g of tea leaves, keeping the humidity at 30%, and then performing microwave pretreatment for 30min at 60 ℃; the microwave power is 200W;
(2) adding the tea leaves pretreated by the microwave into a reaction kettle for steam penetration; in the steam penetration operation, the steam temperature is 100 ℃, the pressure is 2.0Pa, and the time is 5 min; after steam penetrates and receives the mixture, ball milling slurry is obtained by adopting a mechanical ball milling mode, in the mechanical ball milling process, a mode of mixing large balls and small balls is adopted, the diameter of the large balls is 10mm, the diameter of the small balls is 5mm, the ball milling rotation speed is 400rpm, the ball milling time is 5h, and the ball-to-material ratio is 15: 1;
(3) adding ball grinding materials and 100g of pure water into a reaction kettle; then adding citric acid to adjust the pH value of the solution to 6, controlling the temperature at 50 ℃, adding 2g of composite hemicellulase, and reacting for 2 h;
(4) after the reaction, 28g of xylose crystals were obtained by plate filtration, ion exchange, decolorization, concentration, crystallization, and drying.
Example 3
A process for preparing xylose by using tea leaves comprises the following steps;
(1) spraying water to 30kg of tea leaves residue to keep the humidity at 30%, and then performing microwave pretreatment for 20min at 50 ℃; the microwave power is 300W;
(2) adding the tea leaves subjected to microwave treatment into a reaction kettle for steam penetration; in the steam penetration operation, the steam temperature is 150 ℃, the pressure is 1.0Pa, and the time is 8 min; obtaining ball-milling slurry by adopting a mechanical ball-milling mode after the steam penetration is finished, wherein in the mechanical ball-milling process, a mode of mixing large balls and small balls is adopted, the diameter of the large balls is 10mm, the diameter of the small balls is 5mm, the ball-milling rotating speed is 500rpm, the ball-milling time is 3h, and the ball-to-material ratio is 20: 1;
(3) adding ball grinding materials and 100kg of pure water into a reaction kettle; then adding citric acid to adjust the pH value of the solution to 5.0, controlling the temperature at 60 ℃, adding 1.5kg of composite hemicellulase, and reacting for 4 hours;
(4) after the reaction, 19.5g of xylose crystals were obtained by plate filtration, ion exchange, decolorization, concentration, crystallization, and drying.
In the embodiment, the raw materials are subjected to expanded production, and the purity and yield of the finally prepared xylose are still high, which indicates that the method has high stability and can be used for industrial production.
Claims (10)
1. A process for preparing xylose by using tea leaves is characterized by comprising the following steps;
(1) spraying water to the tea leaves to keep humidity, and then carrying out microwave pretreatment;
(2) adding the tea leaves pretreated by the microwave into a reaction kettle for steam penetration; obtaining ball-milling slurry by adopting a mechanical ball-milling mode after the steam penetration is finished;
(3) adding the ball-milling slurry and pure water into a reaction kettle, adjusting the pH value of the solution to 5-6, controlling the temperature to be 50-60 ℃, and adding the composite hemicellulase for 2-4 hours;
(4) after the reaction is finished, the xylose is prepared by plate-type filtration, ion exchange, decoloration, concentration, crystallization and drying.
2. The process for preparing xylose from tea leaves as claimed in claim 1, wherein the humidity is 25% to 35%.
3. The process for preparing xylose by using tea leaves as claimed in claim 1, wherein the temperature of the microwave pretreatment is 50 to 60 ℃, the time is 20 to 30min, and the microwave power is 200 to 300W.
4. The process for preparing xylose by using tea leaves as claimed in claim 1, wherein the temperature of the microwave pretreatment is 56 ℃, the time is 25min, and the microwave power is 230W.
5. The process for preparing xylose from tea leaves as claimed in claim 1, wherein the steam temperature for steam penetration is 100 to 150 ℃, the pressure is 1 to 2Pa, and the time is 1 to 10 min.
6. The process for preparing xylose from tea leaves residue as claimed in claim 1, wherein the steam temperature for steam breakthrough is 120 ℃, the pressure is 1.3Pa and the time is 2 min.
7. The process for preparing xylose by using tea leaves as claimed in claim 1, wherein the mass ratio of the tea leaves to the pure water is 0.3:1 to 0.4: 1; and adding citric acid to adjust the pH value of the solution to 5-6.
8. The process for preparing xylose by using tea leaves as claimed in claim 1, wherein the mass ratio of the tea leaves to the pure water is 0.35: 1; citric acid was added to adjust the pH of the solution to 5.8.
9. The process for preparing xylose by using tea leaves as claimed in claim 1, wherein the amount of the composite hemicellulase is 3-5% of the mass of the tea leaves.
10. The process for preparing xylose by using tea leaves as claimed in claim 1, wherein in the mechanical ball milling process, a mode of mixing big balls and small balls is adopted, the diameter of the big balls is 10mm, the diameter of the small balls is 5mm, the ball milling rotation speed is 300-500 rpm, the ball milling time is 1-5 h, and the ball-material ratio is 10: 1-20: 1.
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| CN202011224176.7A CN112210575A (en) | 2020-11-05 | 2020-11-05 | Process for preparing xylose by using tea leaves |
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Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5639793A (en) * | 1979-09-04 | 1981-04-15 | Agency Of Ind Science & Technol | Preparation of xylose |
| WO1991003566A1 (en) * | 1989-09-06 | 1991-03-21 | Cultor Ltd. | A process for the hydrolysis of hemicellulose by immobilized enzymes and a product comprising an immobilized hemicellulolytic enzyme |
| WO2002070753A2 (en) * | 2001-02-28 | 2002-09-12 | Iogen Energy Corporation | Method of processing lignocellulosic feedstock for enhanced xylose and ethanol production |
| CN101948889A (en) * | 2010-09-14 | 2011-01-19 | 山东龙力生物科技股份有限公司 | Method for preparing oligoxylose containing ferulic acid |
| CN102776244A (en) * | 2012-06-21 | 2012-11-14 | 北京化工大学 | Process for producing polyatomic sugar alcohol and lignin by comprehensively using agricultural and forestry wasters of corncobs |
| CN104099378A (en) * | 2014-07-11 | 2014-10-15 | 清华大学 | Method for preparing xylitol with sweet sorghum fermentation residues |
| CN104498559A (en) * | 2014-11-28 | 2015-04-08 | 山东福田药业有限公司 | Clean production process of xylose |
| CN104946803A (en) * | 2015-05-27 | 2015-09-30 | 华南理工大学 | Method for preparing xylose hydrolysis fluid by utilizing oxalic acid mixed ball milling pretreated corncob |
| CN105705649A (en) * | 2013-08-01 | 2016-06-22 | 诺维信公司 | Process for the enzymatic conversion of lignocellulosic biomass |
| CN106498001A (en) * | 2016-10-18 | 2017-03-15 | 山东福田药业有限公司 | A kind of improved clean manufacturing xylose process |
| US20180169173A1 (en) * | 2016-12-16 | 2018-06-21 | Hill's Pet Nutrition, Inc. | Pet Food Compositions |
| CN110438168A (en) * | 2019-08-17 | 2019-11-12 | 浙江金晟环保股份有限公司 | A method of xylitol is synthesized using bagasse biocatalysis |
-
2020
- 2020-11-05 CN CN202011224176.7A patent/CN112210575A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5639793A (en) * | 1979-09-04 | 1981-04-15 | Agency Of Ind Science & Technol | Preparation of xylose |
| WO1991003566A1 (en) * | 1989-09-06 | 1991-03-21 | Cultor Ltd. | A process for the hydrolysis of hemicellulose by immobilized enzymes and a product comprising an immobilized hemicellulolytic enzyme |
| WO2002070753A2 (en) * | 2001-02-28 | 2002-09-12 | Iogen Energy Corporation | Method of processing lignocellulosic feedstock for enhanced xylose and ethanol production |
| CN101948889A (en) * | 2010-09-14 | 2011-01-19 | 山东龙力生物科技股份有限公司 | Method for preparing oligoxylose containing ferulic acid |
| CN102776244A (en) * | 2012-06-21 | 2012-11-14 | 北京化工大学 | Process for producing polyatomic sugar alcohol and lignin by comprehensively using agricultural and forestry wasters of corncobs |
| CN105705649A (en) * | 2013-08-01 | 2016-06-22 | 诺维信公司 | Process for the enzymatic conversion of lignocellulosic biomass |
| CN104099378A (en) * | 2014-07-11 | 2014-10-15 | 清华大学 | Method for preparing xylitol with sweet sorghum fermentation residues |
| CN104498559A (en) * | 2014-11-28 | 2015-04-08 | 山东福田药业有限公司 | Clean production process of xylose |
| CN104946803A (en) * | 2015-05-27 | 2015-09-30 | 华南理工大学 | Method for preparing xylose hydrolysis fluid by utilizing oxalic acid mixed ball milling pretreated corncob |
| CN106498001A (en) * | 2016-10-18 | 2017-03-15 | 山东福田药业有限公司 | A kind of improved clean manufacturing xylose process |
| US20180169173A1 (en) * | 2016-12-16 | 2018-06-21 | Hill's Pet Nutrition, Inc. | Pet Food Compositions |
| CN110438168A (en) * | 2019-08-17 | 2019-11-12 | 浙江金晟环保股份有限公司 | A method of xylitol is synthesized using bagasse biocatalysis |
Non-Patent Citations (2)
| Title |
|---|
| JIANJUN YANG等: "Pharmacological evaluation of tea polysaccharides with antioxidant activity in gastric cancer mice", 《CARBOHYDRATE POLYMERS》 * |
| 郑生宏等: "茶籽壳酸水解制备木糖工艺研究", 《茶叶科学》 * |
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