CN112210494A - Fermentation device for plant cell culture and use method thereof - Google Patents

Abstract

The invention discloses a fermentation device for plant cell culture and a using method thereof. The invention utilizes the circulating oil to heat and cool, thereby effectively controlling the temperature of the internal environment, saving resources, utilizing the water bath to heat the oil, utilizing the high specific heat and the cleanliness of the oil to ensure the constant temperature effect of the temperature and avoiding the pipeline from being blocked by scale generated by the water circulation heating.

Description

Fermentation device for plant cell culture and use method thereof

Technical Field

The invention relates to the field of plant cell culture, in particular to a fermentation device for plant cell culture and a using method thereof.

Background

The cell culture machine is mainly used for equipment for culturing animal and plant cells, can provide constant temperature and sterile environment for an animal and plant cell culture medium, so that cells in the cell culture medium can be stably propagated, and is common equipment in the cell culture industry.

Most cultivators all are some thermostats at present, utilize the heater strip to carry out direct heating up to inside, and the wasting of resources is serious to because the heater strip causes the internal environment dry easily, cause the cultivation effect unsatisfactory to and accuse temperature effect also unsatisfactory.

Disclosure of Invention

The present invention has been made to solve the above problems, and an object of the present invention is to provide a fermentation apparatus for plant cell culture and a method of using the same.

The invention realizes the purpose through the following technical scheme:

a fermentation device for plant cell culture comprises a drawing mechanism, a heating mechanism, an oil path temperature control mechanism and a connecting mechanism, wherein the drawing mechanism is connected to the inner side of an external protection mechanism in a sliding manner;

the external protection mechanism comprises a box body, the lower end of the box body is connected with a base, four universal wheels are mounted at the lower end of the base, an anti-skid pad is placed at the upper end of the box body, and a control panel is arranged on one side of the anti-skid pad;

the drawing mechanism comprises a drawer, the drawer is connected with the box body in a sliding manner, and the front end of the drawer is provided with a handle;

the temperature adjusting mechanism comprises a temperature control plate, the temperature control plate is connected to the lower end of the drawer, a heating oil duct is arranged on the inner side of the temperature control plate, a cooling oil duct is arranged on the inner side of the heating oil duct in a crossed mode, and oil inlet and oil outlet ends of the heating oil duct and the cooling oil duct are respectively provided with a one-way valve;

the heating mechanism comprises a heating box, heating wires, a water storage cavity and a spiral pipe, the heating box is connected to the oil path temperature control mechanism, the heating wires are arranged on the inner side of the box wall of the heating box, the water storage cavity is arranged inside the heating box, the spiral pipe is arranged in the water storage cavity, and the two ends of the spiral pipe are connected with the oil path temperature control mechanism;

the oil way temperature control mechanism comprises a circulating pump, an oil inlet pipe, a refrigeration box and an insulation box, wherein the oil outlet end of the circulating pump is connected with the oil inlet pipe, the oil inlet end of the circulating pump is connected with the oil outlet pipe, the oil inlet pipe is connected with the heating mechanism, the other oil inlet pipe is connected with the inlet of the refrigeration box, the insulation box is arranged on the inner side of the box body, the inner side of the insulation box penetrates through the oil outlet pipe, an oil duct electromagnetic valve is arranged between the oil outlet.

Preferably: the connecting mechanism comprises an insertion pipe, a thimble, an internal oil duct and a jacking spring, the thimble is arranged at the front end of the insertion pipe and is in sliding connection with the insertion pipe, the jacking spring is arranged between the thimble and the insertion pipe, the thimble is provided with the internal oil duct, the insertion groove is formed on the temperature control plate, an oil outlet is arranged between the insertion groove and the temperature control plate, and the inner side of the insertion groove is provided with scale-shaped sealing strips which are arranged in a crossed mode.

So set up, after the intubate injects the insertion groove inboard, pass scale type sealing strip, utilize the sealed effect of scale type sealing strip simultaneously, the thimble pushes up the insertion groove to make inside oil duct and oil outlet pipe intercommunication, make fluid can enter into in the heating oil duct or the cooling oil duct through the intubate.

Preferably: the connecting mechanism comprises an insertion pipe, a thimble, an internal oil duct and a jacking spring, the thimble is arranged at the front end of the insertion pipe and is connected with the insertion pipe in a sliding mode, the jacking spring is arranged between the thimble and the insertion pipe, the internal oil duct is arranged on the thimble, the insertion groove is formed in the temperature control plate, an oil outlet is formed between the insertion groove and the temperature control plate, and the inner side of the insertion groove is provided with alternately arranged cross sealing air bags.

So set up, after the intubate injects the insertion groove inboard, pass the cross seal gasbag, utilize the sealed effect of cross seal gasbag simultaneously, the thimble pushes up the insertion groove to make inside oil duct and oil outlet pipe intercommunication, make fluid can enter into in the heating oil duct or the cooling oil duct through the intubate.

Preferably: the base is connected with the box body and the universal wheels through bolts, and the control panel is connected with the box body through a rotating shaft.

So set up, the box plays sealed and heat preservation effect, and the universal wheel plays the removal effect.

Preferably: the handle is connected with the drawer through a screw, and a sealing strip is arranged between the drawer and the box body.

So set up, the drawer plays the effect of placing.

Preferably: the temperature control plate pass through the screw connection drawer, heating oil duct, cooling oil duct pass through the buckle and connect the temperature control plate, the clearance packing between temperature control plate and heating oil duct, the cooling oil duct has the heat preservation cotton, the check valve passes through threaded connection heating oil duct, cooling oil duct.

So set up, the accuse temperature board plays the installation effect, and the heating oil duct plays through the heating oil effect, and the cooling oil duct plays through the cooling oil effect.

Preferably: the heating wire is embedded in the heating box, and the spiral pipe is connected with the heating box through welding.

So set up, the heater strip plays the heating effect, and the heating cabinet plays the water storage effect to utilize water bath heating oil, thereby utilize the high specific heat of oil and cleanliness to guarantee the constant temperature effect of temperature and avoid utilizing the incrustation scale of hydrologic cycle heating production to block up the pipeline.

Preferably: the oil inlet pipe and the oil outlet pipe are connected with the spiral pipe and the circulating pump through flanges.

So set up, advance oil pipe, go out oil pipe and play the transmission effect, guarantee the leakproofness through flange joint.

Preferably: the oil duct electromagnetic valve is connected with an oil outlet pipe through threads.

So set up, the oil duct solenoid valve plays the control oil circuit and opens and control velocity of flow effect, has guaranteed the leakproofness through threaded connection.

A method of using a fermentation device for plant cell culture, comprising the steps of:

a. according to the culture conditions of different stages, plant cell culture vessels to be cultured are sequentially placed on the inner sides of a plurality of drawers, the plants are placed from low to high according to the temperature requirement, and then the drawers are reset to enable the drawers and the box body to be matched and sealed;

b. starting a heating wire to heat water in the heating box, starting a circulating pump while heating, and circulating oil in the oil inlet pipe, the oil outlet pipe and the spiral pipe to heat the oil in a water bath, so that the temperature is increased;

c. according to the requirement of the temperature of the inner side of each drawer, each oil duct electromagnetic valve is controlled to be opened, then heated oil is introduced into a heating oil duct at the lower end of the drawer to be heated, and the culture vessel is heated and stored, so that the culture effect is achieved, and when the temperature is enough, the flow is controlled by controlling the opening and closing of the oil duct electromagnetic valves, so that the temperature is controlled to be constant;

d. when the first stage is finished and the second stage culture is needed, the heating wires and the valves on the spiral pipes are closed, the refrigerating box is opened, oil is cooled and circulated, and the inner side of the drawer is cooled and treated.

Compared with the prior art, the invention has the following beneficial effects:

1. the circulating oil is utilized to heat and cool, so that the internal environment is effectively controlled in temperature, and resources are saved;

2. utilize the water bath to heat oil, thereby utilize the high specific heat and the cleanliness of oil to guarantee the constant temperature effect of temperature and avoid utilizing the incrustation scale jam pipeline that water circulation heating produced.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

FIG. 1 is a schematic view of a first configuration of a fermentation apparatus for plant cell culture according to the present invention;

FIG. 2 is a schematic view of a second structure of a fermentation apparatus for plant cell culture according to the present invention;

FIG. 3 is a schematic diagram of an oil inlet pipe of a fermentation apparatus for plant cell culture according to the present invention;

FIG. 4 is a schematic view of the structure of a flow line of a fermentation apparatus for plant cell culture according to the present invention;

FIG. 5 is a schematic diagram of a drawing mechanism of a fermentation device for plant cell culture according to the present invention;

FIG. 6 is a schematic diagram of a temperature control mechanism of a fermentation device for plant cell culture according to the present invention;

FIG. 7 is a schematic diagram of a heating mechanism of a fermentation apparatus for plant cell culture according to the present invention;

FIG. 8 is a schematic view of a scale-type sealing strip of a fermentation apparatus for plant cell culture according to the present invention;

FIG. 9 is a schematic diagram of a cross-sealed air bag structure of a fermentation device for plant cell culture according to the present invention.

The reference numerals are explained below:

1. an external protection mechanism; 2. a drawing mechanism; 3. a temperature adjustment mechanism; 4. a heating mechanism; 5. an oil path temperature control mechanism; 6. a connecting mechanism; 11. a box body; 12. a base; 13. a universal wheel; 14. a non-slip mat; 15. a control panel; 21. a drawer; 22. a handle; 31. a temperature control plate; 32. a heating oil duct; 33. a cooling oil duct; 34. a one-way valve; 41. a heating box; 42. heating wires; 43. a water storage cavity; 44. a spiral tube; 51. a circulation pump; 52. an oil inlet pipe; 53. a refrigeration case; 54. a heat preservation box; 55. an oil outlet pipe; 56. an oil duct solenoid valve; 61. inserting a tube; 62. a thimble; 63. an internal oil passage; 64. the spring is tightly propped; 65. inserting the groove; 66. a scale-type seal strip; 67. an oil outlet hole; 611. the air bags are sealed crosswise.

Detailed Description

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, and are used only for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.

The invention will be further described with reference to the accompanying drawings in which:

example 1

As shown in fig. 1-8, a fermentation apparatus for plant cell culture comprises a drawing mechanism 2, a heating mechanism 4, an oil path temperature control mechanism 5 and a connecting mechanism 6, wherein the drawing mechanism 2 is slidably connected to the inner side of an external protection mechanism 1, the bottom of the drawing mechanism 2 is provided with a temperature adjustment mechanism 3, the rear end of the temperature adjustment mechanism 3 is connected with the oil path temperature control mechanism 5 through the connecting mechanism 6, and the oil path temperature control mechanism 5 is connected with the heating mechanism 4;

the external protection mechanism 1 comprises a box body 11, the lower end of the box body 11 is connected with a base 12, four universal wheels 13 are mounted at the lower end of the base 12, an anti-skid pad 14 is placed at the upper end of the box body 11, and a control panel 15 is arranged on one side of the anti-skid pad 14;

the drawing mechanism 2 comprises a drawer 21, the drawer 21 is connected with the box body 11 in a sliding way, and the front end of the drawer 21 is provided with a handle 22;

the temperature adjusting mechanism 3 comprises a temperature control plate 31, the temperature control plate 31 is connected to the lower end of the drawer 21, a heating oil passage 32 is arranged on the inner side of the temperature control plate 31, a cooling oil passage 33 is crosswise arranged on the inner side of the heating oil passage 32, and oil inlet and oil outlet ends of the heating oil passage 32 and the cooling oil passage 33 are respectively provided with a one-way valve 34;

the heating mechanism 4 comprises a heating box 41, heating wires 42, a water storage cavity 43 and a spiral pipe 44, the heating box 41 is connected to the oil path temperature control mechanism 5, the heating wires 42 are arranged on the inner side of the box wall of the heating box 41, the water storage cavity 43 is arranged in the heating box 41, the spiral pipe 44 is arranged in the water storage cavity 43, and two ends of the spiral pipe 44 are connected with the oil path temperature control mechanism 5;

the oil path temperature control mechanism 5 comprises a circulating pump 51, an oil inlet pipe 52, a refrigeration box 53 and an insulation box 54, wherein the oil outlet end of the circulating pump 51 is connected with the oil inlet pipe 52, the oil inlet end of the circulating pump 51 is connected with an oil outlet pipe 55, the oil inlet pipe 52 is connected with the heating mechanism 4, the other oil inlet pipe 52 is connected with the inlet of the refrigeration box 53, the insulation box 54 is arranged on the inner side of the box body 11, the inner side of the insulation box 54 penetrates through the oil outlet pipe 55, an oil path electromagnetic valve 56 is arranged between the oil outlet pipe 55 and the.

Preferably: the connecting mechanism 6 comprises an insertion tube 61, an ejector pin 62, an internal oil passage 63 and a tightening spring 64, wherein the ejector pin 62 is arranged at the front end of the insertion tube 61, the ejector pin 62 is slidably connected with the insertion tube 61, the tightening spring 64 is arranged between the ejector pin 62 and the insertion tube 61, the internal oil passage 63 is arranged on the ejector pin 62, an insertion groove 65 is formed in the temperature control plate 31, an oil outlet 67 is arranged between the insertion groove 65 and the temperature control plate 31, cross-arranged scale-shaped sealing strips 66 are arranged on the inner side of the insertion groove 65, after the insertion tube 61 is inserted into the insertion groove 65, the insertion groove 65 penetrates through the scale-shaped sealing strips 66, and meanwhile, the ejector pin 62 is pressed against the insertion groove 65 by utilizing the sealing effect of the scale-shaped sealing strips 66, so that the internal oil; the base 12 is connected with the box body 11 and the universal wheels 13 through bolts, the control panel 15 is connected with the box body 11 through a rotating shaft, the box body 11 plays a role in sealing and heat preservation, and the universal wheels 13 play a role in moving; the handle 22 is connected with the drawer 21 through a screw, a sealing strip is arranged between the drawer 21 and the box body 11, and the drawer 21 plays a role in placement; the temperature control plate 31 is connected with the drawer 21 through screws, the heating oil duct 32 and the cooling oil duct 33 are connected with the temperature control plate 31 through buckles, heat preservation cotton is filled in gaps among the temperature control plate 31, the heating oil duct 32 and the cooling oil duct 33, the one-way valve 34 is connected with the heating oil duct 32 and the cooling oil duct 33 through threads, the temperature control plate 31 plays a mounting role, the heating oil duct 32 plays a role of heating oil, and the cooling oil duct 33 plays a role of cooling oil; the heating wire 42 is embedded in the heating box 41, the spiral pipe 44 is connected with the heating box 41 through welding, the heating wire 42 plays a heating role, the heating box 41 plays a water storage role, so that oil is heated by using water bath, the constant temperature effect of temperature is ensured by using the high specific heat and the cleanliness of the oil, and the pipeline is prevented from being blocked by scale generated by water circulation heating; the oil inlet pipe 52 and the oil outlet pipe 55 are connected with the spiral pipe 44 and the circulating pump 51 through flanges, the oil inlet pipe 52 and the oil outlet pipe 55 play a transmission role, and the sealing performance is ensured through flange connection; the oil duct electromagnetic valve 56 is connected with the oil outlet pipe 55 through threads, the oil duct electromagnetic valve 56 plays a role in controlling the opening of an oil path and the flow rate, and the sealing performance is guaranteed through threaded connection.

Example 2

As shown in fig. 9, the present embodiment is different from embodiment 1 in that:

the connecting mechanism 6 comprises an insertion tube 61, an ejector pin 62, an internal oil passage 63 and a tightening spring 64, wherein the ejector pin 62 is arranged at the front end of the insertion tube 61, the ejector pin 62 is slidably connected with the insertion tube 61, the tightening spring 64 is arranged between the ejector pin 62 and the insertion tube 61, the internal oil passage 63 is arranged on the ejector pin 62, an insertion groove 65 is formed on the temperature control plate 31, an oil outlet 67 is arranged between the insertion groove 65 and the temperature control plate 31, cross sealing air bags 611 which are alternately arranged are arranged on the inner side of the insertion groove 65, after the insertion tube 61 is inserted into the insertion groove 65, the insertion groove passes through the cross sealing air bags 611, and meanwhile, the ejector pin 62 is pressed against the insertion groove 65 by utilizing the sealing effect of the cross sealing air bags 611, so that the internal oil passage 63 is.

A method of using a fermentation device for plant cell culture, comprising the steps of:

a. according to the culture conditions of different stages, plant cell culture vessels to be cultured are sequentially placed on the inner sides of the plurality of drawers 21 from low to high according to the temperature requirement, and then the drawers 21 are reset to enable the drawers 21 and the box body 11 to be matched and sealed;

b. the heating wire 42 is started to heat the water in the heating box 41, the circulating pump 51 is started while heating, the oil in the oil inlet pipe 52, the oil outlet pipe 55 and the spiral pipe 44 is circulated, and the oil is heated by water bath, so that the temperature is increased;

c. according to the requirement of the temperature of the inner side of each drawer 21, controlling to open each oil channel electromagnetic valve 56, then introducing the heated oil into the heating oil channel 32 at the lower end of the drawer 21 to be heated, and heating and storing the culture vessel, so that the culture effect is achieved, and when the temperature is enough, controlling the flow rate by controlling the opening and closing of the oil channel electromagnetic valve 56, so as to control the constancy of the temperature;

d. when the first stage is finished and the second stage culture is needed, the heating wire 42 and the valves on the spiral pipe 44 are closed, the refrigerating box 53 is opened, the oil is cooled and circulated, and the inner side of the drawer 21 is cooled.

The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed.

Claims (10)

1. A fermentation device for plant cell culture, characterized in that: the drawing mechanism is connected to the inner side of the external protection mechanism in a sliding manner, the bottom of the drawing mechanism is provided with a temperature regulating mechanism, the rear end of the temperature regulating mechanism is connected with the oil path temperature regulating mechanism through the connecting mechanism, and the oil path temperature regulating mechanism is connected with the heating mechanism;

the external protection mechanism comprises a box body, the lower end of the box body is connected with a base, four universal wheels are mounted at the lower end of the base, an anti-skid pad is placed at the upper end of the box body, and a control panel is arranged on one side of the anti-skid pad;

the drawing mechanism comprises a drawer, the drawer is connected with the box body in a sliding manner, and the front end of the drawer is provided with a handle;

the temperature adjusting mechanism comprises a temperature control plate, the temperature control plate is connected to the lower end of the drawer, a heating oil duct is arranged on the inner side of the temperature control plate, a cooling oil duct is arranged on the inner side of the heating oil duct in a crossed mode, and oil inlet and oil outlet ends of the heating oil duct and the cooling oil duct are respectively provided with a one-way valve;

the heating mechanism comprises a heating box, heating wires, a water storage cavity and a spiral pipe, the heating box is connected to the oil path temperature control mechanism, the heating wires are arranged on the inner side of the box wall of the heating box, the water storage cavity is arranged inside the heating box, the spiral pipe is arranged in the water storage cavity, and the two ends of the spiral pipe are connected with the oil path temperature control mechanism;

the oil way temperature control mechanism comprises a circulating pump, an oil inlet pipe, a refrigeration box and an insulation box, wherein the oil outlet end of the circulating pump is connected with the oil inlet pipe, the oil inlet end of the circulating pump is connected with the oil outlet pipe, the oil inlet pipe is connected with the heating mechanism, the other oil inlet pipe is connected with the inlet of the refrigeration box, the insulation box is arranged on the inner side of the box body, the inner side of the insulation box penetrates through the oil outlet pipe, an oil duct electromagnetic valve is arranged between the oil outlet.

2. A fermentation device for plant cell culture according to claim 1, wherein: the connecting mechanism comprises an insertion pipe, a thimble, an internal oil duct and a jacking spring, the thimble is arranged at the front end of the insertion pipe and is in sliding connection with the insertion pipe, the jacking spring is arranged between the thimble and the insertion pipe, the thimble is provided with the internal oil duct, the insertion groove is formed on the temperature control plate, an oil outlet is arranged between the insertion groove and the temperature control plate, and the inner side of the insertion groove is provided with scale-shaped sealing strips which are arranged in a crossed mode.

3. A fermentation device for plant cell culture according to claim 1, wherein: the connecting mechanism comprises an insertion pipe, a thimble, an internal oil duct and a jacking spring, the thimble is arranged at the front end of the insertion pipe and is connected with the insertion pipe in a sliding mode, the jacking spring is arranged between the thimble and the insertion pipe, the internal oil duct is arranged on the thimble, the insertion groove is formed in the temperature control plate, an oil outlet is formed between the insertion groove and the temperature control plate, and the inner side of the insertion groove is provided with alternately arranged cross sealing air bags.

4. A fermentation device for plant cell culture according to claim 1, wherein: the base is connected with the box body and the universal wheels through bolts, and the control panel is connected with the box body through a rotating shaft.

5. A fermentation device for plant cell culture according to claim 1, wherein: the handle is connected with the drawer through a screw, and a sealing strip is arranged between the drawer and the box body.

6. A fermentation device for plant cell culture according to claim 1, wherein: the temperature control plate pass through the screw connection drawer, heating oil duct, cooling oil duct pass through the buckle and connect the temperature control plate, the clearance packing between temperature control plate and heating oil duct, the cooling oil duct has the heat preservation cotton, the check valve passes through threaded connection heating oil duct, cooling oil duct.

7. A fermentation device for plant cell culture according to claim 1, wherein: the heating wire is embedded in the heating box, and the spiral pipe is connected with the heating box through welding.

8. A fermentation device for plant cell culture according to claim 1, wherein: the oil inlet pipe and the oil outlet pipe are connected with the spiral pipe and the circulating pump through flanges.

9. A fermentation device for plant cell culture according to claim 1, wherein: the oil duct electromagnetic valve is connected with an oil outlet pipe through threads.

10. A method of using a fermentation device for plant cell culture, comprising: the method comprises the following steps:

a. according to the culture conditions of different stages, plant cell culture vessels to be cultured are sequentially placed on the inner sides of a plurality of drawers, the plants are placed from low to high according to the temperature requirement, and then the drawers are reset to enable the drawers and the box body to be matched and sealed;

b. starting a heating wire to heat water in the heating box, starting a circulating pump while heating, and circulating oil in the oil inlet pipe, the oil outlet pipe and the spiral pipe to heat the oil in a water bath, so that the temperature is increased;

c. according to the requirement of the temperature of the inner side of each drawer, each oil duct electromagnetic valve is controlled to be opened, then heated oil is introduced into a heating oil duct at the lower end of the drawer to be heated, and the culture vessel is heated and stored, so that the culture effect is achieved, and when the temperature is enough, the flow is controlled by controlling the opening and closing of the oil duct electromagnetic valves, so that the temperature is controlled to be constant;

d. when the first stage is finished and the second stage culture is needed, the heating wires and the valves on the spiral pipes are closed, the refrigerating box is opened, oil is cooled and circulated, and the inner side of the drawer is cooled and treated.

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