CN112191295A - Single cell genome amplification monitor - Google Patents
Single cell genome amplification monitor Download PDFInfo
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- CN112191295A CN112191295A CN202011081534.3A CN202011081534A CN112191295A CN 112191295 A CN112191295 A CN 112191295A CN 202011081534 A CN202011081534 A CN 202011081534A CN 112191295 A CN112191295 A CN 112191295A
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- 238000013412 genome amplification Methods 0.000 title claims abstract description 8
- 230000006698 induction Effects 0.000 claims abstract description 5
- 238000012544 monitoring process Methods 0.000 claims description 36
- 230000005540 biological transmission Effects 0.000 claims description 13
- 238000000926 separation method Methods 0.000 claims description 8
- 230000002146 bilateral effect Effects 0.000 claims description 7
- 238000004891 communication Methods 0.000 claims description 5
- 230000001174 ascending effect Effects 0.000 claims description 2
- 230000003321 amplification Effects 0.000 claims 5
- 238000003199 nucleic acid amplification method Methods 0.000 claims 5
- 238000007789 sealing Methods 0.000 claims 1
- 238000012360 testing method Methods 0.000 description 13
- 239000000243 solution Substances 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000012165 high-throughput sequencing Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000009461 vacuum packaging Methods 0.000 description 1
- 238000012070 whole genome sequencing analysis Methods 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/06—Test-tube stands; Test-tube holders
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F29/00—Mixers with rotating receptacles
- B01F29/30—Mixing the contents of individual packages or containers, e.g. by rotating tins or bottles
- B01F29/32—Containers specially adapted for coupling to rotating frames or the like; Coupling means therefor
- B01F29/321—Containers specially adapted for coupling to rotating frames or the like; Coupling means therefor of test-tubes or the like
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Clinical Laboratory Science (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Accessories For Mixers (AREA)
Abstract
The invention discloses a single cell genome amplification monitor, which comprises a main box body, wherein a lifting cavity is arranged in the main box body, the upper wall of the lifting cavity is communicated with a placing cavity with an upward opening, the right wall of the placing cavity is vertically and slidably connected with a placing box, a vertically through threaded cavity is arranged in the placing box, the left end surface of the placing box is fixedly connected with a supporting block, the supporting block is rotatably connected with a mixing barrel in a rotating manner, a switch cavity with an upward opening and a leftward and rightward symmetry opening is arranged in the mixing barrel, the lower wall of the switch cavity is communicated with a communicating cavity with a downward opening, and an induction mechanism is arranged in the placing box. And provides a separate storage space.
Description
Technical Field
The invention relates to the field of single cell genome, in particular to a single cell genome amplification monitor.
Background
The single cell whole genome sequencing technology is a new technology for amplifying and sequencing whole genome at single cell level, and the principle is that micro whole genome DNA of separated single cells is amplified, and high-throughput sequencing is carried out after a complete genome with high coverage rate is obtained so as to reveal cell population difference and cell evolution relation. Some existing monitoring equipment are difficult to classify and store test tubes, and do not have the function of automatically mixing the solution in the test tubes, so that the functionality is poor, and certain manpower and time need to be consumed.
Disclosure of Invention
The invention aims to provide a single cell genome amplification monitor, which solves the problems of low detection efficiency and low accuracy of a vacuum packaging bag.
The invention is realized by the following technical scheme.
The invention discloses a unicellular genome amplification monitor, which comprises a main box body, wherein a lifting cavity is arranged in the main box body, the upper wall of the lifting cavity is communicated with a placing cavity with an upward opening, the right wall of the placing cavity is vertically and slidably connected with a placing box, a vertically through threaded cavity is arranged in the placing box, the left end surface of the placing box is fixedly connected with a supporting block, the supporting block is rotatably connected with a mixing barrel, the mixing barrel is internally provided with a switch cavity with an upward opening and a bilateral symmetry, the lower wall of the switch cavity is communicated with a communicating cavity with a downward opening, the placing box is internally provided with an induction mechanism, the lower wall of the lifting cavity is in friction connection with a bilaterally symmetrical monitoring barrel, the monitoring barrel is internally provided with an upward opening cavity, the circumference of the opening cavity is communicated with four storage cavities, the lower wall of the opening cavity is communicated with a gear ring cavity, the, be equipped with the bevel gear chamber in the bevel gear case, ring gear chamber lower wall rotates to be connected with upwards extends to the driving shaft of bevel gear intracavity, the driving shaft rotates with the bevel gear case to be connected, sliding connection has the bearing case about the disc outside the bevel gear case, bearing case up end fixedly connected with stopper, be equipped with monitoring mechanism in the monitoring bucket, the lift chamber downside is equipped with the separation and reunion chamber, separation and reunion chamber upper wall rotates to be connected with and runs through lift chamber and upwards extend to place the initiative screw rod of intracavity, separation and reunion chamber lower wall rotates to be connected with and is located initiative screw rod left side and upwards extend to with the mixed gear axle that lift chamber upper wall rotated the connection, disc fixedly connected with straight-teeth gear outside the mixed gear axle, be equipped with clutching mechanism in the separation and.
Preferably, the sensing mechanism comprises clamping springs fixedly connected with the left side wall and the right side wall of the switch cavity, the driving screw is in threaded connection with the threaded cavity, one end of each clamping spring, which is far away from the two side walls of the switch cavity, is fixedly connected with a clamping plate, one end surface of each clamping plate, which is close to the two side walls of the switch cavity, is fixedly connected with a clamping magnet, the left side wall of the switch cavity is fixedly connected with a switch, which is positioned on the lower side of the clamping electromagnet, on the left side, in an initial state, a through cavity, which is positioned on one side of the communicating cavity, which is close to the driving screw and has a downward opening, is arranged in the mixing barrel, a supporting plate, which can seal the communicating cavity, is hinged to the bottom end of one side wall of the through cavity, which is far away from the driving screw, and, the outer circular surface of the mixing barrel is fixedly connected with a gear ring positioned on the lower side of the supporting block.
Preferably, the monitoring mechanism comprises a monitoring motor arranged on the lower side of the monitoring barrel, the upper end surface of the monitoring motor is in power connection with the bottom end of the driving shaft, the upper wall of the bevel gear cavity is communicated with a transmission cavity, one side of the transmission cavity, which is far away from the driving screw, is provided with a screw cavity with an upward opening, the outer circular surface of the top end of the driving shaft is fixedly connected with a driving bevel gear, one side wall of the transmission cavity, which is close to the driving screw, is rotatably connected with a pushing screw extending into the screw cavity in the direction far away from one side of the driving screw, the outer circular surface of one side of the pushing screw, which is located in the transmission cavity, is fixedly connected with a driven bevel gear meshed with the driving bevel gear, the outer circular surface of one side of the pushing screw, which is located in the screw cavity, is in threaded connection with a pushing plate in sliding, the hinge joint of the stop lever and the bevel gear box is provided with the stop lever, and the upper wall of the storage cavity is provided with a camera.
Preferably, monitoring mechanism still includes the driving shaft is located outer disc fixed connection's of one side in the ring gear intracavity initiative straight-teeth gear, ring gear chamber lower wall fixedly connected with is located the back shaft on driving shaft right side, the outer disc of back shaft rotate be connected with initiative straight-teeth gear meshed idler, terminal surface fixedly connected with connecting axle under the bevel gear case, connecting axle terminal surface fixedly connected with the ring gear of idler meshing, be equipped with the decurrent bearing chamber of opening in the bearing case, bearing chamber upper wall intercommunication is equipped with the ascending chamber of stepping down of opening, the curved arc that the stopper left end face articulated have the longitudinal symmetry is embraced tightly the piece, the arc embrace tightly the piece with articulated department between the stopper is equipped with and embraces tightly a torsional spring, bevel gear case up end with fixedly connected with bearing spring between the bearing chamber upper wall.
Preferably, the outer disc of initiative screw rod rotates and is connected with and is located the slider that leads of monitoring bucket upside, the ejector pin of slider up end fixedly connected with bilateral symmetry leads, the gliding chamber of leading of opening decurrent arc and bilateral symmetry is located to the terminal surface under the slider, monitoring bucket up end fixedly connected with lead the curved arc piece of gliding chamber sliding connection.
Preferably, the clutch mechanism comprises a main screw rod arranged on the lower side of the clutch cavity, the upper end surface of the main screw rod is in power connection with the bottom end of the driving screw rod, a driving belt wheel is fixedly connected to the outer circular surface of one side of the driving screw rod, which is positioned in the clutch cavity, a driven belt wheel is fixedly connected to the outer circular surface of one side of the mixing gear shaft, which is positioned in the clutch cavity, a belt is wound between the driven belt wheel and the driving belt wheel, a belt supporting block is fixedly connected to the lower end surface of the clutch cavity, a belt supporting cavity which runs through the belt is arranged in the belt supporting block and supports the belt, a fixed block positioned on the right side of the belt supporting block is fixedly connected to the lower wall of the clutch cavity, a sliding block positioned on the rear side of the, the tensioning device comprises a fixed block and a sliding block, and is characterized in that a tensioning electromagnet is fixedly connected to the rear end face of the fixed block, a tensioning magnetic block corresponding to the tensioning electromagnet is fixedly connected to the front end face of the sliding block, a tensioning shaft is fixedly connected to the upper end face of the sliding block, and a tensioning wheel is rotatably connected to the outer circular face of the tensioning shaft.
The invention has the beneficial effects that: according to the invention, the solutions which are not required to be mixed are stored in a classified manner, the test tubes which are not required to be mixed can be directly stored, and the test tubes which are required to be mixed are stored after being mixed through the gear in the storage process, so that the solutions are convenient to distinguish, and a separate storage space is arranged, so that the phenomenon that the test tubes are mixed during storage is prevented.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the invention, and it is obvious for those skilled in the art that other drawings can be obtained based on these drawings without creative efforts.
FIG. 1 is a schematic structural diagram of an embodiment of the present invention;
FIG. 2 is an enlarged schematic view of the embodiment of the present invention at A in FIG. 1;
FIG. 3 is an enlarged schematic view of the embodiment of the present invention at B in FIG. 1;
FIG. 4 is a schematic view of C-C in FIG. 3 according to an embodiment of the present invention;
FIG. 5 is an enlarged schematic view at D of FIG. 3 according to an embodiment of the present invention;
FIG. 6 is an enlarged schematic view of the embodiment of the present invention at E in FIG. 1;
FIG. 7 is a schematic view of F-F in FIG. 6 according to an embodiment of the present invention.
Detailed Description
The invention will now be described in detail with reference to fig. 1-7, wherein for ease of description the orientations described below are now defined as follows: the up, down, left, right, and front-back directions described below correspond to the up, down, left, right, and front-back directions in the projection relationship of fig. 1 itself.
The single cell genome amplification monitor described in conjunction with the attached fig. 1-7 comprises a main box body 11, wherein a lifting cavity 12 is arranged in the main box body 11, the upper wall of the lifting cavity 12 is communicated with a placing cavity 13 with an upward opening, the right wall of the placing cavity 13 is connected with a placing box 21 in a vertical sliding manner, a threaded cavity 20 which is communicated with the upper and lower portions is arranged in the placing box 21, the left end surface of the placing box 21 is fixedly connected with a supporting block 22, a mixing barrel 23 is rotatably connected with the supporting block 22, a switch cavity 29 with an upward opening and a left and right symmetrical opening is arranged in the mixing barrel 23, a communicating cavity 30 with a downward opening is arranged in the lower wall of the switch cavity 29, an induction mechanism 101 is arranged in the placing box 21, the lower wall of the lifting cavity 12 is connected with a monitoring barrel 38 with a left and right symmetrical opening, an upward opening cavity 63 is arranged in, the lower wall of the opening cavity 63 is communicated with a gear ring cavity 70, the gear ring cavity 70 is rotatably connected with a bevel gear box 43 extending upwards into the opening cavity 63, a bevel gear cavity 44 is arranged in the bevel gear box 43, the lower wall of the gear ring cavity 70 is rotatably connected with a driving shaft 42 extending upwards into the bevel gear cavity 44, the driving shaft 42 is rotatably connected with the bevel gear box 43, the outer circular surface of the bevel gear box 43 is vertically and slidably connected with a bearing box 76, the upper end surface of the bearing box 76 is fixedly connected with a limiting block 79, a monitoring mechanism 102 is arranged in the monitoring barrel 38, the lower side of the lifting cavity 12 is provided with a clutch cavity 17, the upper wall of the clutch cavity 17 is rotatably connected with a driving screw 14 penetrating through the lifting cavity 12 and extending upwards into the placing cavity 13, the lower wall of the clutch cavity 17 is rotatably connected with a mixing gear shaft 15 which is positioned on the left side of the driving screw 14 and extends upwards to be rotatably connected, the outer circular surface of the mixing gear shaft 15 is fixedly connected with a straight gear 16, and a clutch mechanism 103 is arranged in the clutch cavity 17.
Advantageously, the sensing mechanism 101 includes a clamping spring 64 fixedly connected to the left and right side walls of the switch cavity 29, the driving screw 14 is in threaded connection with the threaded cavity 20, one end of the clamping spring 64 far from the two side walls of the switch cavity 29 is fixedly connected to a clamping plate 28, one end surface of the clamping plate 28 near to the two side walls of the switch cavity 29 is fixedly connected to a clamping magnet 27, the left and right side walls of the switch cavity 29 are fixedly connected to a clamping electromagnet 26 corresponding to the clamping magnet 27, in an initial state, the left side wall of the switch cavity 29 is fixedly connected to a switch 25 located on the lower side of the clamping electromagnet 26 on the left side, a through cavity 32 located on one side of the communication cavity 30 near to the driving screw 14 and having a downward opening is arranged in the mixing barrel 23, a supporting plate 31 capable of closing the communication cavity 30 is hinged to the bottom end of one side wall of the through cavity 32, a supporting torsion spring 33 is arranged at the hinged position of the supporting plate 31 and the through cavity 32, and the outer circular surface of the mixing barrel 23 is fixedly connected with a gear ring 24 positioned at the lower side of the supporting block 22.
Advantageously, the monitoring mechanism 102 includes a monitoring motor 41 disposed on the lower side of the monitoring barrel 38, the upper end surface of the monitoring motor 41 is in power connection with the bottom end of the driving shaft 42, the upper wall of the bevel gear cavity 44 is provided with a transmission cavity 48 in communication, one side of the transmission cavity 48 away from the driving screw 14 is provided with a screw cavity 50 with an upward opening, the outer circular surface of the top end of the driving shaft 42 is fixedly connected with a driving bevel gear 45, one side wall of the transmission cavity 48 close to the driving screw 14 is rotatably connected with a pushing screw 47 extending into the screw cavity 50 in the direction away from the driving screw 14, one side outer circular surface of the pushing screw 47 located in the transmission cavity 48 is fixedly connected with a driven bevel gear 46 engaged with the driving bevel gear 45, one side outer circular surface of the pushing screw 47 located in the screw cavity 50 is in threaded connection with a pushing plate 49 slidably connected with the lower wall of the screw cavity 50, in an initial state, a stop lever 65 is hinged to one side end face, away from the driving screw 14, of the bevel gear box 43, a stop lever 66 is arranged at the hinged position of the stop lever 65 and the bevel gear box 43, and a camera 40 is arranged on the upper wall of the storage cavity 39.
Advantageously, the monitoring mechanism 102 further comprises a driving spur gear 72 fixedly connected with the outer circumferential surface of one side of the driving shaft 42 positioned in the gear ring cavity 70, a supporting shaft 73 positioned at the right side of the driving shaft 42 is fixedly connected to the lower wall of the ring gear chamber 70, an idle gear 74 meshed with the driving spur gear 72 is rotatably connected to the outer circumferential surface of the supporting shaft 73, the lower end surface of the bevel gear box 43 is fixedly connected with a connecting shaft 75, the end surface of the connecting shaft 75 is fixedly connected with a gear ring 71 meshed with the idle gear 74, a bearing cavity 83 with a downward opening is arranged in the bearing box 76, the upper wall of the bearing cavity 83 is communicated with a abdicating cavity 77 with an upward opening, the left end surface of the limiting block 79 is hinged with an arc-shaped clasping block 82 which is symmetrical front and back, a clasping block torsion spring 81 is arranged at the hinged position between the arc-shaped clasping block 82 and the limiting block 79, a bearing spring 78 is fixedly connected between the upper end face of the bevel gear box 43 and the upper wall of the bearing cavity 83.
Beneficially, the outer circular surface of the driving screw 14 is rotatably connected with a guide block 34 located on the upper side of the monitoring barrel 38, the upper end surface of the guide block 34 is fixedly connected with a bilaterally symmetrical ejector rod 35, the lower end surface of the guide block 34 is provided with an arc-shaped and bilaterally symmetrical guide sliding cavity 36 with a downward opening, and the upper end surface of the monitoring barrel 38 is fixedly connected with an arc-shaped arc block 37 slidably connected with the guide sliding cavity 36.
Advantageously, the clutch mechanism 103 includes a main screw 18 disposed at the lower side of the clutch cavity 17, the upper end surface of the main screw 18 is in power connection with the bottom end of the driving screw 14, the outer circumferential surface of one side of the driving screw 14 located in the clutch cavity 17 is fixedly connected with a driving pulley 51, the outer circumferential surface of one side of the mixing gear shaft 15 located in the clutch cavity 17 is fixedly connected with a driven pulley 53, a belt 52 is wound between the driven pulley 53 and the driving pulley 51, the lower end surface of the clutch cavity 17 is fixedly connected with a belt supporting block 54, a belt supporting cavity 55 which penetrates left and right and supports the belt 52 is disposed in the belt supporting block 54, a fixed block 58 located at the right side of the belt supporting block 54 is fixedly connected to the lower wall of the clutch cavity 17, and a sliding block 59 located at the rear side of the fixed block 58 is slidably, a tensioning spring 61 is fixedly connected between the fixed block 58 and the sliding block 59, a tensioning electromagnet 60 is fixedly connected on the rear end face of the fixed block 58, a tensioning magnetic block 62 corresponding to the tensioning electromagnet 60 is fixedly connected on the front end face of the sliding block 59, a tensioning shaft 57 is fixedly connected on the upper end face of the sliding block 59, and a tensioning wheel 56 is rotatably connected on the outer circular surface of the tensioning shaft 57.
In the initial state, the clamping spring 64 is in a relaxed state, the tensioning spring 61 is in a relaxed state, the belt 52 is in an untensioned state, the stop lever 66 is in a relaxed state to maintain the stop lever 65 in a horizontal state, the support torsion spring 33 is in a relaxed state to maintain the support plate 31 in a horizontal state, the clasping block torsion spring 81 is in a relaxed state, and the hold down spring 78 is in a relaxed state.
In operation, a test tube without mixed solution is placed on the upper end surface of the right side bearing plate 31 through the right side switch cavity 29, so that the right side left and right clamping plates 28 are pushed to the left and right sides against the thrust of the clamping spring 64, so that the right side switch 25 is pressed, the main screw 18 is started to drive the driving screw 14 to rotate, the placing box 21 is driven to move downwards through the guide sliding of the right wall of the placing cavity 13 and the lifting cavity 12 in threaded connection, when the placing box 21 moves downwards, the left end of the right side bearing plate 31 is pushed upwards against the torsional spring force of the supporting torsional spring 33 through the right side mandril 35, so that the lower side of the communicating cavity 30 is driven to be in a communicating state, at the moment, the clamping magnet block 27 is adsorbed by starting the clamping electromagnet 26, so that the clamping plate 28 is driven to not clamp the test tube, so that the test tube falls down and overcomes the torsional spring force of the clamping block torsional spring 81, so that the, thereby tightening the supporting spring 78 and driving the supporting box 76 to slide downwards by the gravity of the test tube, thereby making the pushing plate 49 extend upwards to the upper side of the supporting box 76, at this time, the monitoring motor 41 is started to drive the driving shaft 42 to rotate, the idle gear 74 is driven to rotate by the rotation of 42 through the meshing, thereby driving the gear ring 71 to rotate through the meshing, the rotation of the gear ring 71 drives the bevel gear box 43 to rotate through the connection of the connecting shaft 75, at this time, the monitoring barrel 38 is driven to rotate by overcoming the friction force through the limit of the storage cavity 39 by the stop lever 65, the driving bevel gear 45 is driven to rotate through the rotation of the driving shaft 42, thereby driving the driven bevel gear 46 to rotate through the meshing, thereby driving the pushing screw 47 to rotate, the pushing screw 47 rotates and drives the pushing plate 49 to move rightwards through the threaded connection so as to push the test tube to, at this time, the falling of the test tube pushes the stop lever 65 to overcome the torsion spring force of the stop lever 66 and turn downward, so that the monitoring barrel 38 is no longer driven to rotate, and at this time, the bevel gear box 43 is continuously driven to rotate, so that the stop lever 65 is driven to directly face the next storage cavity 39.
Place the test tube that will need mixed solution at left side bearing plate 31 up end, be pressed through left side switch 25 this moment, thereby it adsorbs the thrust of tensioning magnet 62 overcoming tensioning spring 61 to drive tensioning wheel 56, thereby drive tensioning wheel 56 with belt 52 tensioning, start main screw 18 this moment and thereby drive screw 14 and rotate, thereby drive pulley 51 and rotate, thereby it rotates to drive driven pulley 53 through the belt 52 transmission, thereby it rotates to drive mixing gear axle 15, thereby drive straight-teeth gear 16 and rotate, when placing case 21 downstream, ring gear 24 can mesh with straight-teeth gear 16, thereby drive left side mixing bucket 23 at supporting shoe 22 internal rotation, thereby mix the solution in the test tube, thereby can put into to the left side and deposit in chamber 39.
The above embodiments are merely illustrative of the technical ideas and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the protection scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.
Claims (6)
1. A single cell genome amplification monitor, includes the main box body, its characterized in that: the upper wall of the lifting cavity is communicated with a placing cavity with an upward opening, the right wall of the placing cavity is connected with the placing box in a vertical sliding mode, a threaded cavity which is communicated from top to bottom is arranged in the placing box, the left end face of the placing box is fixedly connected with a supporting block, a mixing barrel is rotationally connected with the supporting block, a switch cavity with an upward opening and a bilateral symmetry is arranged in the mixing barrel, a communicating cavity with a downward opening is arranged in the lower wall of the switch cavity, an induction mechanism is arranged in the placing box, a monitoring barrel with a bilateral symmetry is connected with the lower wall of the lifting cavity in a friction mode, an upward opening cavity is arranged in the monitoring barrel, four storage cavities are circumferentially communicated with each other, a gear ring cavity is communicated with the lower wall of the opening cavity, a bevel gear box which extends upwards into the opening cavity is rotationally connected with the gear ring cavity, and, ring gear chamber lower wall rotates to be connected with and upwards extends to the driving shaft of bevel gear intracavity, the driving shaft rotates with the bevel gear case to be connected, sliding connection has the bearing case about the outer disc of bevel gear case, bearing case up end fixedly connected with stopper, be equipped with monitoring mechanism in the monitoring bucket, lift chamber downside is equipped with the separation and reunion chamber, separation and reunion chamber upper wall rotates to be connected with and runs through lift chamber and upwards extend to place the initiative screw rod of intracavity, separation and reunion chamber lower wall rotates to be connected with and is located initiative screw rod left side and upwards extend to with the lift chamber upper wall rotates the mixed gear axle of connecting, the outer disc fixedly connected with straight-teeth gear of mixed gear axle, be equipped with clutching mechanism in the separation and reunion intracavity.
2. The single cell genomic amplification monitor of claim 1, wherein: the induction mechanism comprises clamping springs fixedly connected with the left side wall and the right side wall of the switch cavity, the driving screw is in threaded connection with the threaded cavity, one end, far away from the two side walls of the switch cavity, of each clamping spring is fixedly connected with a clamping plate, one side end face, close to the two side walls of the switch cavity, of each clamping plate is fixedly connected with a clamping magnetic block, the left side wall of the switch cavity is fixedly connected with a clamping electromagnet corresponding to the clamping magnetic block, in an initial state, the left side wall of the switch cavity is fixedly connected with a switch positioned on the lower side of the clamping electromagnet on the left side, a through cavity which is positioned on one side, close to the driving screw, of the communication cavity and is downward in opening is arranged in the mixing barrel, a supporting plate capable of sealing the communication cavity is hinged to the bottom end of one side wall, far away, the outer circular surface of the mixing barrel is fixedly connected with a gear ring positioned on the lower side of the supporting block.
3. The single cell genomic amplification monitor of claim 1, wherein: the monitoring mechanism comprises a monitoring motor arranged on the lower side of the monitoring barrel, the upper end face of the monitoring motor is in power connection with the bottom end of the driving shaft, the upper wall of the bevel gear cavity is communicated with a transmission cavity, one side of the transmission cavity, which is far away from the driving screw rod, is provided with a screw rod cavity with an upward opening, the outer circular surface at the top end of the driving shaft is fixedly connected with a driving bevel gear, one side wall of the transmission cavity, which is close to the driving screw rod, is rotatably connected with a pushing screw rod extending into the screw rod cavity in a direction far away from one side of the driving screw rod, the outer circular surface at one side of the pushing screw rod, which is positioned in the transmission cavity, is fixedly connected with a driven bevel gear meshed with the driving bevel gear, the outer circular surface at one side of the pushing screw rod cavity is in threaded connection with a pushing plate in, the hinge joint of the stop lever and the bevel gear box is provided with the stop lever, and the upper wall of the storage cavity is provided with a camera.
4. The single cell genomic amplification monitor of claim 1, wherein: monitoring mechanism still includes the driving shaft is located outer disc fixed connection's of one side of ring gear intracavity initiative straight-teeth gear, ring gear chamber lower wall fixedly connected with is located the back shaft on driving shaft right side, the outer disc of back shaft rotate be connected with initiative straight-teeth gear meshed idler, terminal surface fixedly connected with connecting axle under the bevel gear case, connecting axle terminal surface fixedly connected with idler meshed ring gear, be equipped with the decurrent bearing chamber of opening in the bearing case, bearing chamber upper wall intercommunication is equipped with the ascending chamber of stepping down of opening, the stopper left end face articulates has the curved arc of longitudinal symmetry to embrace tightly the piece, the arc embrace tightly the piece with articulated department between the stopper is equipped with and embraces tightly a torsional spring, bevel gear case up end with fixedly connected with bearing spring between the bearing chamber upper wall.
5. The single cell genomic amplification monitor of claim 1, wherein: the outer disc of initiative screw rod rotates and is connected with and is located the slider that leads of monitoring bucket upside, lead slider up end fixedly connected with bilateral symmetry's ejector pin, lead the gliding chamber of leading of the downward arc of opening and bilateral symmetry of slider lower extreme face locating, monitoring bucket up end fixedly connected with lead the curved arc piece of gliding chamber sliding connection.
6. The single cell genomic amplification monitor of claim 1, wherein: the clutch mechanism comprises a main screw rod arranged on the lower side of the clutch cavity, the upper end surface of the main screw rod is in power connection with the bottom end of the driving screw rod, one outer circular surface of the driving screw rod, which is positioned in the clutch cavity, is fixedly connected with a driving belt pulley, one outer circular surface of the mixed gear shaft, which is positioned in the clutch cavity, is fixedly connected with a driven belt pulley, a belt is wound between the driven belt pulley and the driving belt pulley, the lower end surface of the clutch cavity is fixedly connected with a belt supporting block, a belt supporting cavity which is communicated with the left and the right sides and supports the belt is arranged in the belt supporting block, the lower wall of the clutch cavity is fixedly connected with a fixed block positioned on the right side of the belt supporting block, the lower wall of the clutch cavity is in front and back sliding connection with a sliding, the front end face of the sliding block is fixedly connected with a tensioning magnetic block corresponding to the tensioning electromagnet, the upper end face of the sliding block is fixedly connected with a tensioning shaft, and the outer circular face of the tensioning shaft is rotatably connected with a tensioning wheel.
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| CN202011081534.3A CN112191295B (en) | 2020-10-12 | 2020-10-12 | Single cell genome amplification monitor |
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| CN202011081534.3A CN112191295B (en) | 2020-10-12 | 2020-10-12 | Single cell genome amplification monitor |
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| CN112191295B CN112191295B (en) | 2021-09-14 |
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| CN112191295B (en) | 2021-09-14 |
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