CN112190397A - Traditional Chinese medicine microneedle patch for wound repair and preparation method thereof - Google Patents
Traditional Chinese medicine microneedle patch for wound repair and preparation method thereof Download PDFInfo
- Publication number
- CN112190397A CN112190397A CN202011202934.5A CN202011202934A CN112190397A CN 112190397 A CN112190397 A CN 112190397A CN 202011202934 A CN202011202934 A CN 202011202934A CN 112190397 A CN112190397 A CN 112190397A
- Authority
- CN
- China
- Prior art keywords
- chinese medicine
- traditional chinese
- microneedle
- microneedle patch
- wound repair
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F13/00—Bandages or dressings; Absorbent pads
- A61F13/02—Adhesive plasters or dressings
- A61F13/0276—Apparatus or processes for manufacturing adhesive dressings or bandages
- A61F13/0289—Apparatus or processes for manufacturing adhesive dressings or bandages manufacturing of adhesive dressings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F13/00—Bandages or dressings; Absorbent pads
- A61F13/02—Adhesive plasters or dressings
- A61F13/0203—Adhesive plasters or dressings having a fluid handling member
- A61F13/0213—Adhesive plasters or dressings having a fluid handling member the fluid handling member being a layer of hydrocoloid, gel forming material
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F13/00—Bandages or dressings; Absorbent pads
- A61F13/02—Adhesive plasters or dressings
- A61F13/0246—Adhesive plasters or dressings characterised by the skin adhering layer
- A61F13/0253—Adhesive plasters or dressings characterised by the skin adhering layer characterized by the adhesive material
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/42—Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
- A61K9/0021—Intradermal administration, e.g. through microneedle arrays, needleless injectors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/70—Web, sheet or filament bases ; Films; Fibres of the matrix type containing drug
- A61K9/7023—Transdermal patches and similar drug-containing composite devices, e.g. cataplasms
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M37/00—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin
- A61M37/0015—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin by using microneedles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F13/00—Bandages or dressings; Absorbent pads
- A61F2013/00361—Plasters
- A61F2013/00727—Plasters means for wound humidity control
- A61F2013/00748—Plasters means for wound humidity control with hydrocolloids or superabsorbers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F13/00—Bandages or dressings; Absorbent pads
- A61F2013/00361—Plasters
- A61F2013/00902—Plasters containing means
- A61F2013/00906—Plasters containing means for transcutaneous or transdermal drugs application
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F13/00—Bandages or dressings; Absorbent pads
- A61F13/02—Adhesive plasters or dressings
- A61F13/0276—Apparatus or processes for manufacturing adhesive dressings or bandages
- A61F2013/0296—Apparatus or processes for manufacturing adhesive dressings or bandages for making transdermal patches (chemical processes excluded)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M37/00—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin
- A61M37/0015—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin by using microneedles
- A61M2037/0023—Drug applicators using microneedles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M37/00—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin
- A61M37/0015—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin by using microneedles
- A61M2037/0053—Methods for producing microneedles
Abstract
The invention discloses a traditional Chinese medicine microneedle patch for wound surface repair and a preparation method thereof. The traditional Chinese medicine microneedle patch prepared by the invention is composed of a biodegradable hydrogel material and a traditional Chinese medicine extract, the hydrogel material can continuously release the traditional Chinese medicine extract to a wound in the degradation process so as to promote wound healing, and the preparation method is simple and easy to implement, has an obvious curative effect and is wide in application range.
Description
Technical Field
The invention relates to the technical field of biomedical materials, in particular to a traditional Chinese medicine microneedle patch for wound repair and a preparation method thereof.
Background
Accidental abrasions and violent injuries often cause severe skin damage and form open wounds, and particularly cause severe septicemia and even death after bacterial infection, so wound repair is an important problem which is continuously concerned and urgently needed to be solved in the field of global medical health. The wound surface is usually repaired by methods such as suturing, medical dressing coating, bandage and the like, and the methods adopt single drug effect, the dressing is not degradable, the dressing needs to be replaced for many times, the wound healing is not facilitated, and the treatment effect needs to be improved.
Microneedle patches have received a great deal of attention as a multifunctional patch, and have achieved remarkable success in the fields of wound healing, disease treatment, biosensing, skin immunization, and the like. Compared with the conventional drug carrier, the microneedle patch has a well-designed microstructure and superior drug carrying capacity, and can realize loading and release of active drugs. However, microneedle patches in widespread use today are generally prepared by complicated chemical synthesis involving a series of chemical reagents and complicated processes, and these chemically synthesized high molecular materials increase the risk of side effects of the microneedle patches.
Therefore, in the invention, a traditional Chinese medicine microneedle patch for wound repair and a preparation method thereof are designed and invented by a template replication method, the traditional Chinese medicine microneedle patch adopts traditional Chinese medicine extracts and natural hydrogel as raw materials, no chemical reagent or harsh synthetic environment is involved in the preparation process, the natural pharmacological activity of the traditional Chinese medicine extracts is ensured, in practical application, the traditional Chinese medicine microneedle patch is directly attached to a skin wound, and the traditional Chinese medicine extracts can be continuously released to the wound in the degradation process of the hydrogel material, so that the wound healing is promoted.
Disclosure of Invention
The invention aims to solve the technical problem of providing a traditional Chinese medicine microneedle patch for wound repair and a preparation method thereof aiming at the defects of the prior art.
In order to achieve the technical purpose, the technical scheme adopted by the invention is as follows:
a traditional Chinese medicine microneedle patch for wound repair is prepared by the following steps:
(1) preparing a microneedle raw material: weighing a proper amount of traditional Chinese medicine extract, adding the traditional Chinese medicine extract into the hydrogel solution, and fully stirring; adding a photoinitiator, and curing to obtain a uniform microneedle raw material;
(2) preparing a traditional Chinese medicine microneedle patch: filling the microneedle raw material obtained in the step (1) into a microneedle template by using a centrifugal method, and preparing a traditional Chinese medicine microneedle patch after ultraviolet irradiation; the traditional Chinese medicine microneedle patches with different needle body appearances can be prepared by selecting different microneedle templates.
Furthermore, the traditional Chinese medicine extract is from Chinese herbal medicine centella.
Further, the hydrogel solution is selected from one or more of methacrylate gelatin, methacrylate hyaluronic acid and methacrylate chitosan.
Further, the photoinitiator is 2-hydroxy-2-methyl propiophenone, and the addition volume percentage of the photoinitiator in the hydrogel solution is 1%.
Further, the hydrogel solution needs to be placed in the dark before curing.
Further, the microneedle body is conical or triangular pyramid-shaped.
Furthermore, the height of the conical or triangular pyramid-shaped microneedle body is 800-.
Further, the wavelength of the ultraviolet light irradiated by the ultraviolet light is 365 nm.
In the step (1), the ratio of the traditional Chinese medicine extract to the hydrogel solution is 1: 20.
in the step (2), the centrifugation conditions are as follows: centrifuge at 3500rpm for 5 min.
The traditional Chinese medicine microneedle patch disclosed by the invention adopts traditional Chinese medicine extracts and degradable hydrogel as raw materials, and in practical application, the traditional Chinese medicine microneedle patch is directly attached to a skin wound surface, and the hydrogel material can continuously release the traditional Chinese medicine extracts to the wound in the degradation process so as to promote the wound surface to heal.
Compared with the prior art, the invention has the beneficial effects that:
1) the traditional Chinese medicine microneedle patch for wound surface repair is prepared by a template copying method, the structure of a microneedle body is controllable, the method is simple and easy to implement, complex instruments and operation means are not needed, the precision is high, and the repeatability is good;
2) the traditional Chinese medicine microneedle patch for wound repair is prepared by taking traditional Chinese medicine extracts and degradable hydrogel as raw materials, does not relate to harmful chemical reagents or complex synthetic environment, and ensures the natural pharmacological activity of the traditional Chinese medicine extracts;
3) the traditional Chinese medicine microneedle patch for wound repair provided by the invention has good biocompatibility, can be directly attached to a skin wound, and can continuously release a traditional Chinese medicine extract at the wound along with the decrease of a hydrogel material, exert the pharmacological characteristics of the traditional Chinese medicine microneedle patch and accelerate the wound repair.
Drawings
Fig. 1 is a flow chart of a preparation process of the traditional Chinese medicine microneedle patch for wound repair.
Fig. 2 is a real image of the traditional Chinese medicine microneedle patch of the present invention, wherein fig. a is a whole real image photographed by a camera, and fig. b is a needle body image observed under a body type microscope.
Fig. 3 is a scanning electron microscope characterization view of the traditional Chinese medicine microneedle patch in example 1.
Fig. 4 is a study on biocompatibility of the traditional Chinese medicine microneedle patch of example 3, in which fig. a is a result of cell staining and fig. b is a result of cell proliferation rate.
FIG. 5 is a staining pattern of tissue sections of the control group and the experimental group in example 4, in which FIG. a is an HE staining pattern, FIG. b is a Masson staining pattern, and FIG. c is a blood vessel immunostaining pattern.
Detailed Description
In order to make those skilled in the art better understand the technical solution of the present invention, the following detailed description of the embodiments of the present invention is provided with reference to the accompanying drawings.
The experimental procedures used in the examples below are, unless otherwise specified, conventional procedures and the reagents, methods and equipment used are, unless otherwise specified, conventional in the art.
The traditional Chinese medicine microneedle patch for wound surface repair is prepared by a template copying method, the structure of a microneedle body is controllable, the method is simple and easy to implement, complex instruments and operation means are not needed, and the traditional Chinese medicine microneedle patch is high in precision and good in repeatability.
The traditional Chinese medicine microneedle patch disclosed by the invention adopts traditional Chinese medicine extracts and natural hydrogel as raw materials, does not relate to chemical reagents or harsh synthetic environment, ensures the natural pharmacological activity of the traditional Chinese medicine extracts, and in practical application, the traditional Chinese medicine microneedle patch is directly attached to a skin wound surface, and a hydrogel material can continuously release the traditional Chinese medicine extracts to the wound in a degradation process so as to promote the wound surface to heal.
The following are examples:
example 1
A traditional Chinese medicine microneedle patch using methacrylate hyaluronic acid as a raw material is prepared by the following steps, wherein the preparation process is shown in figure 1:
(1) preparing microneedle material
Preparing 2% methacrylate hyaluronic acid aqueous solution, and after completely dissolving the methacrylate hyaluronic acid aqueous solution; adding Chinese medicinal asiatic acid solid powder with final concentration of 0.5 μ M into the solution, and stirring thoroughly; finally, adding 1 percent by volume of photoinitiator 2-hydroxy-2-methyl propiophenone into the mixed solution, uniformly mixing to obtain a microneedle raw material, and storing in a dark place;
(2) preparation of traditional Chinese medicine microneedle patch
Filling the microneedle raw material prepared in the step (1) into a designed microneedle mould, and centrifuging for 5min under the condition of 3500rpm to ensure that the microneedle body is completely filled with the microneedle raw material; then irradiating for 30s under ultraviolet light to solidify the microneedle raw material; finally, carefully peeling the solidified traditional Chinese medicine microneedle patch from the microneedle mould;
(3) characterization of traditional Chinese medicine microneedle patch for wound repair
Observing and characterizing the traditional Chinese medicine micro-needle patch prepared in the step (2) under a body type microscope and a scanning electron microscope, wherein fig. 2 is a real object image of the traditional Chinese medicine micro-needle patch, wherein a is an overall real object image shot by a camera, and b is a needle body image observed under the body type microscope; fig. 3 is a scanning electron microscope characterization diagram of the traditional Chinese medicine microneedle patch, and as can be seen from fig. 3, the needle body has a good structure, the needle body is conical, the height of the microneedle needle body is 800 μm, the diameter of the bottom of the needle body is 220 μm, and the diameter of the needle tip is 20 μm.
Example 2
A traditional Chinese medicine microneedle patch taking methacrylate gelatin as a raw material is prepared according to the following method:
(1) preparing microneedle material
Preparing a methacrylate gelatin aqueous solution with the mass concentration of 20% and completely dissolving the methacrylate gelatin aqueous solution; adding Chinese medicinal asiatic acid solid powder with final concentration of 0.5 μ M into the solution, stirring and dissolving; finally, adding 1 percent by volume of photoinitiator 2-hydroxy-2-methyl propiophenone into the mixed solution, uniformly mixing to obtain a microneedle raw material, and storing in a dark place;
(2) preparation of traditional Chinese medicine microneedle patch
Filling the microneedle raw material prepared in the step (1) into a designed microneedle mould, and centrifuging for 5min under the condition of 3500rpm to ensure that the microneedle body is completely filled with the microneedle raw material; then irradiating for 20s under ultraviolet light to solidify the microneedle raw material; and finally, carefully peeling the cured traditional Chinese medicine microneedle patch from the microneedle mould.
(3) Characterization of traditional Chinese medicine microneedle patch for wound repair
The traditional Chinese medicine micro-needle patch prepared in the step (2) is observed and characterized under a body type microscope and a scanning electron microscope, the needle body structure is good, the needle body is triangular pyramid, the height of the micro-needle body is 900 micrometers, the diameter of the bottom of the needle body is 250 micrometers, and the diameter of the needle point is 20 micrometers.
Example 3
The traditional Chinese medicine micro-needle patch for wound repair has biocompatibility:
(1) cell culture
Selecting fibroblast NIH-3T3 as a tested cell line, placing a cell culture medium which is a DMEM high-sugar medium containing 10% fetal calf serum and 1% double antibody (penicillin and streptomycin) into a carbon dioxide incubator for culture, wherein the temperature of the carbon dioxide incubator is 37 ℃, the carbon dioxide content is 5%, the saturation humidity is high, the liquid can be changed every two days when the cell number is small, the liquid is changed every day after entering a logarithmic growth phase, and the cells are digested and passaged by 0.25% of pancreatin when the cell number is about 90%.
(2) Traditional Chinese medicine microneedle patch biocompatibility research for wound repair
Taking the traditional Chinese medicine microneedle patch prepared in example 1 as an example, the traditional Chinese medicine microneedle patch is placed in a culture dish, washed 3 times by using 75% absolute ethyl alcohol, then placed in an ultra-clean workbench for ultraviolet sterilization overnight, washed 3 times by using 1 × PBS every other day, the absolute ethyl alcohol is completely removed, then the traditional Chinese medicine microneedle patch is transferred to a new culture dish, the traditional Chinese medicine microneedle patch is washed 1-2 times by using the cell culture medium prepared in step 1, and finally the traditional Chinese medicine microneedle patch is transferred to a 6-well plate. After cell digestion counting, 2mL of cell suspension (5 x 10) was aspirated4cells/mL) is added into a 6-well plate containing a traditional Chinese medicine microneedle patch, which is an experimental group, a control group is used for cell culture under the same condition, the control group and the experimental group are respectively provided with at least 6 repetitions, calcein is added into 3 repeated 6-well plates after 24h and 48h of co-culture to stain and observe cell morphology by a laser confocal microscope, 800 muL of thiazole blue solution (MTT, 3mg/mL) is added into the other 3 repeated 6-well plates, the plates are put back into a carbon dioxide incubator to be continuously cultured for 3-4h, then the liquid in the plates is sucked out, 800 muL of dimethyl sulfoxide (DMSO) is added, and the dimethyl sulfoxide (DMSO) is gently addedAnd (3) shaking the pore plate until the blue-violet crystals are completely dissolved, finally measuring the light absorption value at 490nm by using an enzyme-labeling instrument, calculating the cell proliferation rate, and evaluating the cell biocompatibility of the traditional Chinese medicine microneedle patch. Fig. 4 shows the result of the biocompatibility study of the microneedle patch, in which fig. a shows the result of cell staining and fig. b shows the result of cell proliferation rate, and it can be seen from fig. 4 that the microneedle patch prepared in example 1 has good biocompatibility.
Example 4
The traditional Chinese medicine microneedle patch for wound repair acts on the rat wound:
taking the traditional Chinese medicine microneedle patch prepared in example 1 as an example, 12 SD male rats weighing about 220g are purchased, 10% (w/v) chloral hydrate is injected into the abdominal cavity to be anesthetized, and then a circular skin total defect model with the diameter of 1cm is constructed on the backs of the rats;
the rats with the wound on the back are randomly divided into 2 groups, a control group and an experimental group, wherein each group comprises 6 rats, and the wound of the rats in the control group is not treated; the experimental group rat wound surface is attached with a traditional Chinese medicine microneedle patch, the outer side of the traditional Chinese medicine microneedle patch is fixed by an adhesive tape and is continuously attached for 9 days, and then a sample is taken from the back wound surface of the rat and is subjected to immunostaining;
the immunostaining results are shown in fig. 5, and the results show that the thickness of the granulation tissue of the experimental group is significantly larger than that of the control group, which indicates that the traditional Chinese medicine microneedle patch can promote the regeneration of the granulation tissue; in addition, the Masson staining result also shows that compared with a control group, the collagen deposition of the experimental group is more, and the traditional Chinese medicine microneedle patch can effectively promote the formation of the new collagen; the result of the vascularization immunostaining shows that the number of the new vessels in the experimental group is obviously more than that in the control group, which indicates that the traditional Chinese medicine microneedle patch has the effect of promoting angiogenesis. To sum up, the traditional Chinese medicine microneedle patch can effectively promote wound repair, and compared with self repair of a control group, the traditional Chinese medicine microneedle patch has good capability of promoting wound repair.
The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention in any way, and any person skilled in the art can make any simple modification, equivalent replacement, and improvement on the above embodiment without departing from the technical spirit of the present invention, and still fall within the protection scope of the technical solution of the present invention.
Claims (10)
1. A traditional Chinese medicine microneedle patch for wound repair is characterized in that: the preparation method comprises the following steps:
(1) preparing a microneedle raw material: weighing a proper amount of traditional Chinese medicine extract, adding the traditional Chinese medicine extract into the hydrogel solution, and fully stirring; adding a photoinitiator, and curing to obtain a uniform microneedle raw material;
(2) preparing a traditional Chinese medicine microneedle patch: filling the microneedle raw material obtained in the step (1) into a microneedle template by using a centrifugal method, and preparing a traditional Chinese medicine microneedle patch after ultraviolet irradiation; the traditional Chinese medicine microneedle patches with different needle body appearances can be prepared by selecting different microneedle templates.
2. A traditional Chinese medicine microneedle patch for wound repair according to claim 1, characterized in that: the Chinese medicinal extract is from centella asiatica.
3. A traditional Chinese medicine microneedle patch for wound repair according to claim 1, characterized in that: the hydrogel solution is selected from one or more of methacrylate gelatin, methacrylate hyaluronic acid and methacrylate chitosan.
4. A traditional Chinese medicine microneedle patch for wound repair according to claim 1, characterized in that: the photoinitiator is 2-hydroxy-2-methyl propiophenone, and the addition volume percentage of the photoinitiator in the hydrogel solution is 1%.
5. A traditional Chinese medicine microneedle patch for wound repair according to claim 1, characterized in that: the hydrogel solution needs to be kept in the dark before curing.
6. A traditional Chinese medicine microneedle patch for wound repair according to claim 1, characterized in that: the microneedle body is conical or triangular pyramid-shaped.
7. A traditional Chinese medicine microneedle patch for wound repair as claimed in claim 6, wherein: the height of the conical or triangular pyramid-shaped microneedle body is 800-.
8. A traditional Chinese medicine microneedle patch for wound repair according to claim 1, characterized in that: the wavelength of ultraviolet light irradiated by the ultraviolet light is 365 nm.
9. A traditional Chinese medicine microneedle patch for wound repair according to claim 1, characterized in that: in the step (1), the ratio of the traditional Chinese medicine extract to the hydrogel solution is 1: 20.
10. a traditional Chinese medicine microneedle patch for wound repair according to claim 1, characterized in that: in the step (2), the centrifugation conditions are as follows: centrifuge at 3500rpm for 5 min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011202934.5A CN112190397A (en) | 2020-11-02 | 2020-11-02 | Traditional Chinese medicine microneedle patch for wound repair and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011202934.5A CN112190397A (en) | 2020-11-02 | 2020-11-02 | Traditional Chinese medicine microneedle patch for wound repair and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112190397A true CN112190397A (en) | 2021-01-08 |
Family
ID=74011155
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011202934.5A Pending CN112190397A (en) | 2020-11-02 | 2020-11-02 | Traditional Chinese medicine microneedle patch for wound repair and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112190397A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113041211A (en) * | 2021-04-07 | 2021-06-29 | 中国人民解放军军事科学院军事医学研究院 | Preparation method and application of MOF (Metal organic framework) microneedle patch |
| CN113069683A (en) * | 2021-04-07 | 2021-07-06 | 中国人民解放军军事科学院军事医学研究院 | Preparation method of soluble microneedle patch for wound repair |
| CN113144272A (en) * | 2021-02-07 | 2021-07-23 | 南京鼓楼医院 | Shape-adjustable hydrogel patch for wound repair and preparation method thereof |
| CN114231632A (en) * | 2021-12-20 | 2022-03-25 | 深圳大学 | DNA hydrogel microneedle patch capable of stimulating response and preparation method and application thereof |
| CN115025276A (en) * | 2022-06-21 | 2022-09-09 | 武汉大学中南医院 | Photo-crosslinking microneedle material containing cationic salt and preparation method and application thereof |
| CN116350583A (en) * | 2023-03-30 | 2023-06-30 | 沈阳药科大学 | Zinc oxide nano particle/ionic liquid dispersion with synergistic antibacterial effect and preparation and application thereof |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1252272A (en) * | 1998-07-30 | 2000-05-10 | 莱雅公司 | Plaster for beauty, medicine or skin science |
| CN108578540A (en) * | 2018-06-05 | 2018-09-28 | 深圳市旷逸生物科技有限公司 | A kind of biologic formulation and preparation method thereof for inhibiting scar and promoting wound healing |
| CN108721204A (en) * | 2018-06-04 | 2018-11-02 | 南京紫源康医药科技有限公司 | A kind of solubility alkannin microneedle patch and preparation method thereof |
| CN108837299A (en) * | 2018-07-18 | 2018-11-20 | 武汉大学 | A kind of intelligence adjusts the microneedle patch and preparation method thereof of blood glucose |
| CN109675185A (en) * | 2019-01-02 | 2019-04-26 | 浙江大学 | Quick microneedle patch of a kind of phenyl boric acid water-setting matrix sugar and preparation method thereof |
| CN110897997A (en) * | 2019-12-31 | 2020-03-24 | 广州贝奥吉因生物科技股份有限公司 | Dextran grafted methacrylic acid hydrogel microneedle and preparation method thereof |
| CN111388407A (en) * | 2020-04-17 | 2020-07-10 | 南京鼓楼医院 | Microneedle array based on dopamine gel and preparation and application thereof |
| CN111408047A (en) * | 2020-04-17 | 2020-07-14 | 南京鼓楼医院 | Conductive microneedle patch for wound repair and preparation method thereof |
| CN111450042A (en) * | 2020-04-17 | 2020-07-28 | 南京鼓楼医院 | Controllable oxygen carrier microneedle and application thereof |
| CN111467668A (en) * | 2020-04-17 | 2020-07-31 | 南京鼓楼医院 | Microneedle array with substrate provided with micro sucker structure and preparation method and application thereof |
| CN111568887A (en) * | 2020-06-23 | 2020-08-25 | 云南省药物研究所 | Bulleyaconitine A dissolvable microneedle patch and preparation method thereof |
-
2020
- 2020-11-02 CN CN202011202934.5A patent/CN112190397A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1252272A (en) * | 1998-07-30 | 2000-05-10 | 莱雅公司 | Plaster for beauty, medicine or skin science |
| CN108721204A (en) * | 2018-06-04 | 2018-11-02 | 南京紫源康医药科技有限公司 | A kind of solubility alkannin microneedle patch and preparation method thereof |
| CN108578540A (en) * | 2018-06-05 | 2018-09-28 | 深圳市旷逸生物科技有限公司 | A kind of biologic formulation and preparation method thereof for inhibiting scar and promoting wound healing |
| CN108837299A (en) * | 2018-07-18 | 2018-11-20 | 武汉大学 | A kind of intelligence adjusts the microneedle patch and preparation method thereof of blood glucose |
| CN109675185A (en) * | 2019-01-02 | 2019-04-26 | 浙江大学 | Quick microneedle patch of a kind of phenyl boric acid water-setting matrix sugar and preparation method thereof |
| CN110897997A (en) * | 2019-12-31 | 2020-03-24 | 广州贝奥吉因生物科技股份有限公司 | Dextran grafted methacrylic acid hydrogel microneedle and preparation method thereof |
| CN111388407A (en) * | 2020-04-17 | 2020-07-10 | 南京鼓楼医院 | Microneedle array based on dopamine gel and preparation and application thereof |
| CN111408047A (en) * | 2020-04-17 | 2020-07-14 | 南京鼓楼医院 | Conductive microneedle patch for wound repair and preparation method thereof |
| CN111450042A (en) * | 2020-04-17 | 2020-07-28 | 南京鼓楼医院 | Controllable oxygen carrier microneedle and application thereof |
| CN111467668A (en) * | 2020-04-17 | 2020-07-31 | 南京鼓楼医院 | Microneedle array with substrate provided with micro sucker structure and preparation method and application thereof |
| CN111568887A (en) * | 2020-06-23 | 2020-08-25 | 云南省药物研究所 | Bulleyaconitine A dissolvable microneedle patch and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 杨丹等: "《桂北药用植物资源现代研究》", 31 March 2019 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113144272A (en) * | 2021-02-07 | 2021-07-23 | 南京鼓楼医院 | Shape-adjustable hydrogel patch for wound repair and preparation method thereof |
| CN113041211A (en) * | 2021-04-07 | 2021-06-29 | 中国人民解放军军事科学院军事医学研究院 | Preparation method and application of MOF (Metal organic framework) microneedle patch |
| CN113069683A (en) * | 2021-04-07 | 2021-07-06 | 中国人民解放军军事科学院军事医学研究院 | Preparation method of soluble microneedle patch for wound repair |
| CN114231632A (en) * | 2021-12-20 | 2022-03-25 | 深圳大学 | DNA hydrogel microneedle patch capable of stimulating response and preparation method and application thereof |
| CN114231632B (en) * | 2021-12-20 | 2023-09-22 | 深圳大学 | DNA hydrogel microneedle patch capable of stimulating response and preparation method and application thereof |
| CN115025276A (en) * | 2022-06-21 | 2022-09-09 | 武汉大学中南医院 | Photo-crosslinking microneedle material containing cationic salt and preparation method and application thereof |
| CN115025276B (en) * | 2022-06-21 | 2023-09-19 | 武汉大学中南医院 | Photo-crosslinking microneedle material containing cationic salt and preparation method and application thereof |
| CN116350583A (en) * | 2023-03-30 | 2023-06-30 | 沈阳药科大学 | Zinc oxide nano particle/ionic liquid dispersion with synergistic antibacterial effect and preparation and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112190397A (en) | Traditional Chinese medicine microneedle patch for wound repair and preparation method thereof | |
| Zhou et al. | Rapid printing of bio-inspired 3D tissue constructs for skin regeneration | |
| CN109913400B (en) | Artificial tissue precursors and methods for making same | |
| Shafiee et al. | Convergence of 3D printed biomimetic wound dressings and adult stem cell therapy | |
| CN109985279B (en) | Micro-patterned nanofiber material compounded with drug-loaded MOF (Metal organic framework), and preparation method and application thereof | |
| CN112569399B (en) | Photo-crosslinking/electrostatic spinning preparation and application of hydrogel composite scaffold with double-layer skin structure | |
| He et al. | Manufacture of PLGA multiple-channel conduits with precise hierarchical pore architectures and in vitro/vivo evaluation for spinal cord injury | |
| Ajalloueian et al. | One-stage tissue engineering of bladder wall patches for an easy-to-use approach at the surgical table | |
| Kates et al. | Tissue-engineered urinary conduits | |
| CN112675360B (en) | Preparation and application of hADSCs-loaded double-layer skin bionic hydrogel composite scaffold | |
| CN112870453B (en) | Gelatin-type III collagen hydrogel and preparation method and application thereof | |
| Liu et al. | A novel use of genipin-fixed gelatin as extracellular matrix for peripheral nerve regeneration | |
| Yang et al. | Sciatic nerve repair by reinforced nerve conduits made of gelatin–tricalcium phosphate composites | |
| Ma et al. | 3D bioprinting of a gradient stiffened gelatin–alginate hydrogel with adipose-derived stem cells for full-thickness skin regeneration | |
| Chen et al. | Robot-assisted in situ bioprinting of gelatin methacrylate hydrogels with stem cells induces hair follicle-inclusive skin regeneration | |
| CN114129506A (en) | Asiaticoside-carrying microneedle and application thereof in promoting wound healing | |
| CN114904056A (en) | Composite hydrogel based on human placenta acellular matrix and preparation method thereof | |
| Dong et al. | Electrospun nanofibrous membranes of recombinant human collagen type III promote cutaneous wound healing | |
| JP5935181B2 (en) | Biomaterial for wound healing and its preparation | |
| Zhang et al. | Chitosan nerve conduit filled with ZIF-8-functionalized guide microfibres enhances nerve regeneration and sensory function recovery in sciatic nerve defects | |
| CN112426568B (en) | Tissue engineering scaffold based on lotus roots and preparation method and application thereof | |
| Du et al. | Strontium-doped mesoporous bioglass nanoparticles for enhanced wound healing with rapid vascularization | |
| Majd et al. | The Possibility of Diabetic Wound Healing Using Electro spun PLA Nano Fibers | |
| Mohammadi et al. | Fabrication and Evaluation of Gelatin-based Nanofibrous Wound Dressings incorporated with therapeutic algae extract for potential wound healing | |
| CN117510911A (en) | Neural biological printing ink and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210108 |