CN112179880A - Rapid diagnosis method for water-soluble organic matter source of drinking water source - Google Patents
Rapid diagnosis method for water-soluble organic matter source of drinking water source Download PDFInfo
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Abstract
The invention belongs to the technical field of water quality detection, and particularly relates to a rapid diagnosis method for a water soluble organic matter source of a drinking water source. The main components of the fluorescent DOM are humus and protein-like sources, which approximately represent the main sources of the DOM in the water body, and the water source is high in protection degree, so that the organic pollution condition is relatively simple compared with other rivers, and a premise is provided for quickly diagnosing the DOM sources in the water body according to the existence conditions of the humoid and the protein-like. The fluorescence spectrum, also called as Excited Emission Matrix Spectra (EEMs), can quickly and accurately obtain the information of the composition, concentration and the like of DOC fluorescence components of the water body without damaging the structure of a sample. Meanwhile, the fluorescence characterization method for rapidly analyzing the DOC components is accurate, relatively cheap and has important significance on a large number of sampling processes in time and space, and can effectively and comprehensively track the distribution, composition and change rule of the DOC in the water body and the influence on the ecological environment.
Description
Technical Field
The invention belongs to the technical field of water quality detection, and particularly relates to a rapid diagnosis method for a water soluble organic matter source of a drinking water source.
Background
Water is a source of life, and the existence of liquid water is lacked, so that no possibility of life exists. The water status is particularly important in human life and production activities as an important strategy and material basis for sustainable development. For drinking water which is closely related to people, the drinking water is a necessity of human life and is an irreplaceable product. However, in recent years, with the rapid development of economic society, a large amount of domestic sewage and industrial water are discharged into rivers and lakes, so that the physical and mental health of human beings is seriously threatened. Aerobic organic pollutants such as azo materials, amines, phenols, bromates, bacteria, viruses, heavy metal ions and the like contained in drinking water source ground water cause water body malodor, and the water quality is seriously deteriorated. To ensure the rational utilization of water resources and the sustainable development of economy, to maintain human health, how to monitor and remove these toxic and harmful substances has become important to environmental workers.
The Dissolved Organic Matter (DOM) is one of water quality indexes reflecting the pollution of water bodies by organic matters, is a combination of carbon-containing organic matters, has complex and various components, and consists of mixtures of amino acids, carbohydrates, aromatic compounds, macromolecular humic acid and the like in different quantities. The existence of large amounts of dissolved organic matter presents a great challenge to the traditional water treatment process. The river DOM can represent the organic matter content and the biological activity level in the river water body, reflect the water pollution degree, and record the change of human activities (such as forest and grassland destruction, large-scale mechanical cultivation, mining, irrigation, dam building, cross-river basin water transfer, urbanization and the like) to the river basin environment. Therefore, the main source of analyzing the river DOM is exogenous input or endogenous release, and the method has obvious environmental significance for preventing and controlling organic pollution in the river domain.
The method for monitoring the pollutants of the soluble organic matters in the water of the drinking water source at the present stage mainly comprises the following steps: gas chromatography, liquid chromatography, ion chromatography, each of which has its own advantages and disadvantages. Different substances are different in type and property, detection means are different, and conditions for chromatographic and mass spectrometric detection must be adjusted for different concentrations. In the face of increasingly complex water environments, the aim of effective detection can be well achieved by combining a plurality of methods, but the subsequent investment cost is correspondingly high. For a new method, relatively speaking, the detectable substances are single, the water sample is easy to damage, the accuracy is low, and the speed is low, so that the method is difficult to widely popularize and apply. The detection of harmful substances in drinking water sources is still under constant research and investigation. Disclosure of Invention
The technical problems to be solved by the invention are as follows: aiming at the problems of easy water sample damage, low accuracy and low speed of the existing detection method, the rapid diagnosis method for the water soluble organic matter source of the drinking water source is provided.
In order to solve the technical problems, the invention adopts the following technical scheme:
a rapid diagnosis method for water-soluble organic matter sources of a drinking water source comprises the following steps:
(1) 80 parts of drinking water source water sample is taken and detected by a fluorescence spectrometer, and a three-dimensional fluorescence spectrum is obtained;
(2) decomposing the three-dimensional fluorescence spectrum, comparing the spectral characteristics of all components with the three-dimensional fluorescence spectrum results of other lakes and rivers, confirming the representative types of the components, and identifying 3 fluorescence components;
(3) the 3 fluorescent components are respectively C1, C2 and C3, wherein the fluorescence T peak of C1 is (260/440 nm), the fluorescence T peak of C2 is (230, 275/340 nm), the fluorescence T peak of C3 is (245/400 nm), C1 and C3 belong to the humoid fluorescent component, and C2 is a typical protein-like fluorescent component;
(4) 3, analyzing the fluorescence component to obtain the information of the DOC fluorescence component of the water body, such as the composition, the concentration and the like, without damaging the structure of the sample, thereby quickly diagnosing the source of the water-soluble organic matters of the drinking water source.
C1 in the step (3) has 1 excitation peak and 1 emission peak.
C1 mainly reflects the fluorescence property of short-wavelength humus, which is similar to the A peak of traditional terrestrial humus and the M peak of marine humus, wherein the A peak represents the fluorescence peak of short-wavelength humus with HA as the main component; m peak is fluorescence peak of sea-derived long-wave humus, which is used as important index of primary productivity and vitality of water body, and C3 represents terrestrial humus.
The tryptophan single body has 2 excitation peaks and 1 emission peak, is consistent with a fluorescence T peak (225-230, 270-280/340-350 nm) of a traditional tryptophan single body, and represents an endogenous dissolved organic matter.
Compared with other methods, the method has the beneficial technical effects that:
the main components of the fluorescent DOM are humus and protein-like sources, which approximately represent the main sources of the DOM in the water body, and the water source is high in protection degree, so that the organic pollution condition is relatively simple compared with other rivers, and a premise is provided for quickly diagnosing the DOM sources in the water body according to the existence conditions of the humoid and the protein-like. The fluorescence spectrum, also called as Excited Emission Matrix Spectra (EEMs), can quickly and accurately obtain the information of the composition, concentration and the like of DOC fluorescence components of the water body without damaging the structure of a sample. Meanwhile, the fluorescence characterization method for rapidly analyzing the DOC components is accurate, relatively cheap and has important significance on a large number of sampling processes in time and space, and can effectively and comprehensively track the distribution, composition and change rule of the DOC in the water body and the influence on the ecological environment.
Drawings
FIG. 1 shows the fluorescent composition C1;
FIG. 2 shows the fluorescent composition C2;
FIG. 3 shows the fluorescent composition C3.
Detailed Description
A rapid diagnosis method for water-soluble organic matter sources of a drinking water source comprises the following steps:
(1) 80 parts of drinking water source water sample is taken and detected by a fluorescence spectrometer, and a three-dimensional fluorescence spectrum is obtained;
(2) decomposing the three-dimensional fluorescence spectrum, comparing the spectral characteristics of all components with the three-dimensional fluorescence spectrum results of other lakes and rivers, confirming the representative types of the components, and identifying 3 fluorescence components;
(3) the 3 fluorescent components are respectively C1, C2 and C3, wherein the fluorescence T peak of C1 is (260/440 nm), the fluorescence T peak of C2 is (230, 275/340 nm), the fluorescence T peak of C3 is (245/400 nm), C1 and C3 belong to the humoid fluorescent component, and C2 is a typical protein-like fluorescent component;
(4) 3, analyzing the fluorescence component to obtain the information of the DOC fluorescence component of the water body, such as the composition, the concentration and the like, without damaging the structure of the sample, thereby quickly diagnosing the source of the water-soluble organic matters of the drinking water source.
C1 in the step (3) has 1 excitation peak and 1 emission peak.
C1 mainly reflects the fluorescence property of short-wavelength humus, which is similar to the A peak of traditional terrestrial humus and the M peak of marine humus, wherein the A peak represents the fluorescence peak of short-wavelength humus with HA as the main component; m peak is fluorescence peak of sea-derived long-wave humus, which is used as important index of primary productivity and vitality of water body, and C3 represents terrestrial humus.
The tryptophan single body has 2 excitation peaks and 1 emission peak, is consistent with a fluorescence T peak (225-230, 270-280/340-350 nm) of a traditional tryptophan single body, and represents an endogenous dissolved organic matter.
Examples
A rapid diagnosis method for water-soluble organic matter sources of a drinking water source comprises the following steps:
(1) 80 parts of drinking water source water sample is taken and detected by a fluorescence spectrometer, and a three-dimensional fluorescence spectrum is obtained;
(2) decomposing the three-dimensional fluorescence spectrum, comparing the spectral characteristics of all components with the three-dimensional fluorescence spectrum results of other lakes and rivers, confirming the representative types of the components, and identifying 3 fluorescence components;
(3) the 3 fluorescent components are respectively C1, C2 and C3, wherein the fluorescence T peak of C1 is (260/440 nm), the fluorescence T peak of C2 is (230, 275/340 nm), the fluorescence T peak of C3 is (245/400 nm), C1 and C3 belong to the humoid fluorescent component, and C2 is a typical protein-like fluorescent component;
(4) 3, analyzing the fluorescence component to obtain the information of the DOC fluorescence component of the water body, such as the composition, the concentration and the like, without damaging the structure of the sample, thereby quickly diagnosing the source of the water-soluble organic matters of the drinking water source.
C1 in the step (3) has 1 excitation peak and 1 emission peak.
C1 mainly reflects the fluorescence property of short-wavelength humus, which is similar to the A peak of traditional terrestrial humus and the M peak of marine humus, wherein the A peak represents the fluorescence peak of short-wavelength humus with HA as the main component; m peak is fluorescence peak of sea-derived long-wave humus, which is used as important index of primary productivity and vitality of water body, and C3 represents terrestrial humus.
The tryptophan single body has 2 excitation peaks and 1 emission peak, is consistent with a fluorescence T peak (225-230, 270-280/340-350 nm) of a traditional tryptophan single body, and represents an endogenous dissolved organic matter.
Wherein, the spectral characteristics of the 3 fluorescent components are as follows:
in conclusion, the detection method is rapid and accurate, does not damage a water sample, and is worthy of popularization, meanwhile, the parallel factor analysis is one of statistical methods, the maximum fluorescence intensity of each fluorescent component of DOM in the water body can be analyzed in detail based on Matlab by combining fluorescence spectrum, and the DOM in the water body is mainly from an endogenous source or an exogenous source through calculating the specific gravity of each component relative to the fluorescence intensity. 80 water samples are grouped according to possible factors influencing DOM in the environment, such as different land utilization types (woodland, farmland and city), each group is used as 1 influencing factor, fluorescent data of each component of DOM under different groups is analyzed by combining with an SPSS general linear model, the influence degree of each component influenced by each environmental factor can be judged according to the coefficient size and the significance of each factor in the model, and the specific input source of the DOM and the influence of the environmental factors on the DOM source can be roughly judged according to the influence degrees of the factors, so that a basis is provided for corresponding preventive and control measures aiming at organic matter pollution of water bodies.
Claims (4)
1. A rapid diagnosis method for a source of water-soluble organic matters of a drinking water source is characterized by comprising the following steps:
(1) 80 parts of drinking water source water sample is taken and detected by a fluorescence spectrometer, and a three-dimensional fluorescence spectrum is obtained;
(2) decomposing the three-dimensional fluorescence spectrum, comparing the spectral characteristics of all components with the three-dimensional fluorescence spectrum results of other lakes and rivers, confirming the representative types of the components, and identifying 3 fluorescence components;
(3) the 3 fluorescent components are respectively C1, C2 and C3, wherein the fluorescence T peak of C1 is (260/440 nm), the fluorescence T peak of C2 is (230, 275/340 nm), the fluorescence T peak of C3 is (245/400 nm), C1 and C3 belong to the humoid fluorescent component, and C2 is a typical protein-like fluorescent component;
(4) 3, analyzing the fluorescence component to obtain the information of the DOC fluorescence component of the water body, such as the composition, the concentration and the like, without damaging the structure of the sample, thereby quickly diagnosing the source of the water-soluble organic matters of the drinking water source.
2. The rapid diagnosis method for water-soluble organic matter source of drinking water source as claimed in claim 1, wherein C1 in step (3) has 1 excitation peak and 1 emission peak.
3. The method for rapidly diagnosing a source of a water-soluble organic substance in a drinking water source as claimed in claim 2, wherein C1 mainly reflects the fluorescence property of the short wavelength humus-like substance, similar to the conventional A peak of the terrestrial humus-like substance and M peak of the marine humus-like substance, wherein the A peak represents the fluorescence peak of the short wavelength humus-like substance containing HA as a main component; m peak is fluorescence peak of sea-derived long-wave humus, which is used as important index of primary productivity and vitality of water body, and C3 represents terrestrial humus.
4. The method for rapidly diagnosing the source of the soluble organic matters in the drinking water source according to claim 1, wherein the source of the soluble organic matters in the drinking water source has 2 excitation peaks and 1 emission peak, is consistent with a fluorescence T peak (225-230, 270-280/340-350 nm) of a traditional tryptophan monomer, and represents the endogenous soluble organic matters.
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| CN112881353A (en) * | 2021-01-11 | 2021-06-01 | 江西师范大学 | Method and device for measuring concentration of soluble organic carbon in water body |
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| 陈永娟;胡玮璇;庞树江;王晓燕;: "北运河水体中荧光溶解性有机物空间分布特征及来源分析", 环境科学, no. 08, pages 1 - 2 * |
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| CN112881353A (en) * | 2021-01-11 | 2021-06-01 | 江西师范大学 | Method and device for measuring concentration of soluble organic carbon in water body |
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