CN112179737A - Tumour pathological section dewatering device - Google Patents
Tumour pathological section dewatering device Download PDFInfo
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- CN112179737A CN112179737A CN202011057869.1A CN202011057869A CN112179737A CN 112179737 A CN112179737 A CN 112179737A CN 202011057869 A CN202011057869 A CN 202011057869A CN 112179737 A CN112179737 A CN 112179737A
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- timer
- guide rod
- soaking
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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Abstract
The invention relates to a tumor pathological section dehydration device, which effectively solves the problems of multiple dehydration steps of pathological tissues, long time, high labor intensity and the like; the technical scheme for solving the problem is that the device comprises a base, wherein a cylinder body is fixedly arranged at the center of the base, a rotatable shaft is arranged at the center of the cylinder body, and a helical blade and a helical groove are arranged on the outer edge surface of the cylinder body; a sleeve is arranged on the rotating shaft, a cross rod is arranged on the sleeve, a vertical guide rod is inserted at the outer end of the cross rod, a first permanent magnet is arranged at the upper end of the guide rod, and a placing box is arranged at the lower end of the guide rod; soaking pools with upward openings are arranged on the upper side surfaces of the helical blades at equal intervals from bottom to top, a timer is fixed at the front end of each soaking pool, a plurality of fixing blocks are arranged on the barrel, limiting rods are inserted into the fixing blocks, and second permanent magnets are fixed at the upper ends of the limiting rods; a plurality of electromagnets corresponding to the second permanent magnets one by one are fixed on the lower side surface of the helical blade; the electromagnet is powered off after the timer returns to zero; the invention has the advantages of mechanical automation, convenient adjustment, simplicity and easy operation.
Description
Technical Field
The invention relates to the field of tumor pathological analysis, in particular to a dehydration device for a tumor pathological section.
Background
In pathological analysis of tumors, paraffin sections are required to be prepared for observation and study. The paraffin section method comprises the steps of material taking, fixing, washing and dehydrating, transparentizing, wax dipping, embedding, section and patch making, dewaxing, dyeing, dehydrating, transparentizing, sealing and the like. In the dehydration treatment of pathological tissues, alcohol is a commonly used dehydrating agent, which can be mixed with both water and a clearing agent. To reduce the abrupt contraction of the tissue material, it should be carried out using a sequence of increasing concentrations from low to high, typically starting with 30% or 50% alcohol, over 70%, 85%, 95% up to pure alcohol (absolute ethanol), each time thirty minutes or even hours. The tissue mass is of different sizes, so that the dehydration time in each step is different, and the dehydration time is not long enough.
At present, when tissue blocks are soaked, most of the tissue blocks are still manually soaked, and the soaking liquid is regularly replaced by inspectors; the dehydration step requires a long time for each step, which increases the workload of the examiner and is liable to cause hardening due to the tissue soaking for a long time due to the time loss. Some devices adopt mechanical arms to perform timed and automatic tissue replacement of soaking liquid, but the equipment has high cost, inconvenient maintenance and use, complicated device adjustment and high learning cost, so the device is not configured in most hospitals. In view of the foregoing, there is a need for a simpler apparatus for effecting the dehydration of tissue masses.
Disclosure of Invention
In view of the above situation, the present invention provides a tumor pathological section dehydration device, which can effectively solve the problems of multiple pathological tissue dehydration steps, long time, high labor intensity, etc., in the prior art.
The technical scheme for solving the problem is that the device comprises a base, wherein a vertical cylinder is fixedly arranged in the center of the base, a rotating shaft capable of rotating is inserted in the center of the cylinder, and a torsion spring is arranged between the lower end of the rotating shaft and the base; the outer edge surface of the cylinder body is provided with a helical blade, and the cylinder body is provided with a helical groove corresponding to the helical blade; the rotating shaft is sleeved with a sleeve which can only move up and down along the rotating shaft, a cross rod is fixed on the sleeve, the outer end of the cross rod penetrates through the spiral groove and is arranged on the outer side of the barrel body, a vertical guide rod is inserted into the outer end of the cross rod, and the guide rod can move up and down and reset upwards; the upper end of the guide rod is provided with a first permanent magnet, and the lower end of the guide rod is fixedly provided with a placing box;
soaking pools with upward openings are arranged on the upper side surfaces of the spiral blades at equal intervals from bottom to top, a timer is fixed at the front end of each soaking pool, a plurality of fixing blocks which correspond to the soaking pools one by one are arranged on the barrel, limiting rods which can move up and down and reset upwards are inserted in the fixing blocks, and the limiting rods can block the transverse rods from moving upwards along the spiral grooves after moving downwards; a second permanent magnet is fixed at the upper end of the limiting rod; a plurality of electromagnets corresponding to the second permanent magnets one by one are fixed on the lower side surface of the helical blade; each timer controls the electromagnet right above the timer, and the electromagnet is powered off after the timer returns to zero.
The invention has the following characteristics:
1. the number of the soaking pools required to be adopted can be selected by adjusting the timer;
2. the device can be vibrated to enable the low-concentration soaking liquid attached to the device and tissues to drip;
3. the liquid which is poured out can be collected into the lowest groove along the helical blade;
4. after soaking, the water can automatically rise to the top, so that the state can be conveniently determined;
5. after a plurality of soaking pools are arranged, the occupied area is still small;
6. the mechanical automation is realized, the adjustment is convenient, the operation is simple and easy, and the soaking process can be accelerated or repeated at any time;
7. and according to the located height, quickly recognizing the located process.
Drawings
Fig. 1 is a schematic perspective view i of the present invention.
Fig. 2 is a schematic perspective view ii of the present invention.
Fig. 3 is a front view of the present invention.
Fig. 4 is a front sectional view of the present invention (with wedge removed).
Fig. 5 is a cross-sectional view of the timepiece.
Detailed Description
The following describes in further detail embodiments of the present invention with reference to the accompanying drawings.
As shown in fig. 1 to 5, the present invention comprises a base 1, a vertical cylinder 2 is fixedly installed in the center of the base 1, a rotatable rotating shaft 3 is inserted in the center of the cylinder 2, and a torsion spring 4 is installed between the lower end of the rotating shaft 3 and the base 1; the outer edge surface of the cylinder body 2 is provided with a helical blade 5, and the cylinder body 2 is provided with a helical groove 6 corresponding to the helical blade 5; a sleeve 7 which can only move up and down along the rotating shaft 3 is sleeved on the rotating shaft 3, a cross rod 8 is fixed on the sleeve 7, the outer end of the cross rod 8 penetrates through the spiral groove 6 and is arranged on the outer side of the barrel body 2, a vertical guide rod 9 is inserted at the outer end of the cross rod 8, and the guide rod 9 can move up and down and reset upwards; the upper end of the guide rod 9 is provided with a first permanent magnet 10, and the lower end of the guide rod 9 is fixed with a placing box 11;
soaking pools 12 with upward openings are arranged on the upper side surfaces of the spiral blades 5 at equal intervals from bottom to top, a timer 13 is fixed at the front end of each soaking pool 12, a plurality of fixing blocks 14 which correspond to the soaking pools 12 one by one are arranged on the barrel 2, limiting rods 15 which can move up and down and reset upwards are inserted in the fixing blocks 14, and the limiting rods 15 can block the cross rods 8 from moving upwards along the spiral grooves 6 after moving downwards; a second permanent magnet 16 is fixed at the upper end of the limiting rod 15; a plurality of electromagnets 17 corresponding to the second permanent magnets 16 one by one are fixed on the lower side surface of the helical blade 5; each timer 13 controls the electromagnet 17 directly above it, and after the timer 13 has been reset to zero, the electromagnet 17 is de-energized.
In order to realize that the timer 13 controls the on-off of the electromagnet 17, the timer 13 comprises a shell 18, a rotatable pointer 19 and a reset pointer 19 are mounted at the center of the shell 18, a first electrode plate 20 is wrapped at the upper end of the pointer 19, and a fan-shaped second electrode plate 21 is mounted on the shell 18; the second electrode avoids the zero graduation line right above the timer 13; when the hand 19 is rotated, the first electrode piece 20 comes into contact with the second electrode piece 21, and the electromagnet 17 corresponding to the timer 13 is energized.
In order to make the residual soaking liquid on the tissue block quickly drop, a plurality of wedge blocks 22 positioned between two adjacent electromagnets 17 are uniformly distributed on the lower side surface of the helical blade 5.
In order to collect and treat the residual liquid more conveniently, the base 1 is provided with an annular groove 23, and the outer edges of the helical blades 5 are provided with baffle pieces 24.
In order to realize the up-and-down movement of the sleeve 7 along the rotating shaft 3, the cross section of the rotating shaft 3 is square, and the upper end of the rotating shaft 3 is provided with a knob 25.
In order to realize the up-and-down movement and upward reset of the guide rod 9 and the limiting rod 15, the guide rod 9 is sleeved with a first tension spring 26 positioned between the first permanent magnet 10 and the cross rod 8, and the limiting rod 15 is sleeved with a second tension spring 27 positioned between the second permanent magnet 16 and the fixing block 14.
For the tissue mass soaking, the placing box 11 is made of stainless steel filter screen.
When the invention is used, firstly, alcohol soak solutions with gradually increased alcohol content are respectively added into the plurality of soaking pools 12 from bottom to top. Because the number of the soaking pools 12 is multiple from bottom to top, when the number of required soaking solutions is small, namely the soaking steps are small, only a part of the soaking pools 12 are needed; the specific application is not strictly limited, and the specific application is preferably ordered from bottom to top, so that subsequent observation is facilitated.
Then placing the pathological tissues in the placing box 11, and sealing the placing box 11; in an initial state, because the torsion spring 4 is arranged between the rotating shaft 3 and the base 1, the sleeve 7 can move up and down along the rotating shaft 3, and the cross rod 8 on the sleeve 7 is arranged in the spiral groove 6 of the barrel 2, the cross rod 8 is positioned at the uppermost end of the spiral groove 6 under the action of the torsion force of the torsion spring 4; the sleeve 7 is now also above. After the tissue block is placed, the rotating shaft 3 needs to be rotated clockwise (in a overlooking state), and the rotating shaft 3 is square, so that the sleeve 7 descends along the spiral groove 6 due to the rotation of the rotating shaft 3; until the placing box 11 at the lower end of the guide rod 9 corresponds to the lowest soaking pool 12, the rotation of the rotating shaft 3 is stopped at the moment.
During the above-mentioned downward progressive movement of the sleeve 7, it is necessary to manually press the guide rod 9, preventing the upper end of the guide rod 9 from being blocked by the wedge 22, thus affecting the descent.
At this time, the position of the rotating shaft 3 needs to be kept, the rotating shaft 3 is prevented from being reset under the action of the torsion spring 4, then the timer 13 on the bottommost soaking pool 12 is rotated, taking the first soaking pool 12 for soaking for 1 hour as an example, the timer 13 on the first soaking pool 12 is rotated to the position of 1 hour; when the timer 13 rotates, the first electrode piece 20 on the pointer 19 in the timer 13 contacts with the second electrode piece 21 on the outer shell 18, so that the electromagnet 17 positioned right above the soaking pool 12 is electrified; when the electromagnet 17 is energized, the first permanent magnet 10 and the second permanent magnet 16 are pushed to move downward simultaneously.
After the first permanent magnet 10 moves downwards, the guide rod 9 moves downwards at the moment, so that the placing box 11 at the lower end of the guide rod 9 is soaked in the soaking pool 12 at the lowest part; after the second permanent magnet 16 moves downwards, the limiting rod 15 is pushed to move downwards, so that the lower end of the limiting rod 15 blocks the cross rod 8, and the cross rod 8 cannot rise along the spiral groove 6; after which the spindle 3 can be released.
Referring to fig. 1, the placing box 11 is positioned in the bottommost soaking pool 12; since the timer 13 of the first steeping cistern 12 (the lowermost steeping cistern 12) is set to 1 hour, if the second steeping cistern 12 needs to be steeped for one hour, the second timer 13 is set to 2 hours, and similarly, if the third steeping cistern 12 needs to be steeped for 30 minutes, the third timer 13 is set to two and a half hours, and so on, the time for the tissue blocks to be steeped in each steeping cistern 12 is the current timer 13 minus the previous timer 13.
After the rest of the timers 13 are set, the electromagnet 17 right above the timer 13 with the set time is in an electrified state, and the limiting rod 15 corresponding to the electrified electromagnet 17 is located at the lowest part of the stroke, namely, the transverse rod 8 can be blocked from moving upwards.
When the time of the first soaking pool 12 reaches, the timer 13 returns to zero, the electromagnet 17 corresponding to the first soaking pool 12 is powered off, and the first permanent magnet 10 and the second permanent magnet 16 lose repulsive force; the limiting rod 15 and the guide rod 9 can be reset upwards; after the limiting rod 15 is reset upwards, the limiting rod 15 does not block the cross rod 8, and at the moment, the cross rod 8 can move upwards along the spiral groove 6 under the action of the rotating shaft 3, the torsion spring 4 and the sleeve 7; after the guide rod 9 is reset up and down, the guide rod 9 is reset upwards at the moment, and the placing box 11 at the lower end of the guide rod 9 is separated from the first soaking pool 12.
In the process that the cross rod 8 rises along the spiral groove 6, because the plurality of wedge blocks 22 are uniformly distributed between two adjacent electromagnets, along with the rising of the cross rod 8, the upper end of the guide rod 9 can be continuously stirred by the plurality of wedge blocks 22, so that the guide rod 9 continuously vibrates up and down, the soaking liquid attached to the placing box 11 and the tissue block can drip down and can drip on the spiral blade 5, and then the soaking liquid continuously flows downwards along the spiral blade 5 and finally flows into the groove 23 of the base 1.
Because the timer 13 on the second soaking pool 12 is still in a timing state, the electromagnet 17 corresponding to the second soaking pool 12 is in an energized state, and at this time, the limiting rod 15 at the position is in a state of blocking the cross rod 8, so that the cross rod 8 can reach the position of the second soaking pool 12 after rising along the spiral groove 6 and stop, and simultaneously, under the action of the electromagnet, the first permanent magnet 10 at the upper end of the guide rod 9 can also receive repulsive force, so that the placing box 11 at the lower end of the guide rod 9 is soaked into the second soaking pool 12.
By analogy, the tissue blocks can be sequentially soaked in the soaking pools 12 finally, and the automatic sequential soaking effect of the tissue blocks is realized.
After the soaking is finished, the tissue block is separated from the last soaking pool 12, at the moment, because the subsequent timer 13 is in time, the subsequent electromagnet 17 is in a power-off state, the cross rod 8 can ascend along the spiral groove 6 all the way and finally stay at the top, and the whole process is finished.
After each use, the whole device can be repeatedly used after being cleaned and disinfected.
This device is through setting up helical blade 5 and helicla flute 6, utilizes helicla flute 6 to play the effect to the horizontal pole 8 direction, makes horizontal pole 8 along the ascending gradually of helicla flute 6, and helical blade 5's the side of going up is provided with soaking pond 12, and the downside is provided with electro-magnet 17, through the upper and lower mutually supporting, can make electro-magnet 17 promote guide arm 9, makes organize the piece and soak to soaking pond 12 in, has realized both ingenious combinations.
This device is through setting up time-recorder 13 and electro-magnet 17 to and the design of gag lever post 15, through the time-recorder 13 of the fermentation vat 12 that will soak and open, and gag lever post 15 that corresponds with this fermentation vat 12 this moment can be in the state that blocks, makes horizontal pole 8 drive the stay in each fermentation vat 12 that the piece can once organize, realizes automatic soaking.
This device arranges a plurality of steeping ponds 12 along helical blade 5 through the spiral, has reduced area, can realize more soaking steps, improves the effect of soaking.
In the device, because the timer 13 is installed on each soaking pool 12, the length of the soaking time of each soaking pool 12 can be adjusted at any time by rotating the timer 13, so that reasonable adjustment is facilitated.
The design of wedge 22 in this device can make the continuous vibrations of guide arm 9 to the soaking liquid of low concentration is dripped on making placing box 11, makes the raffinate drip on helical blade 5, and descends to the recess 23 of base 1 along helical blade 5 in, has realized the automatic collection of waste liquid.
Claims (7)
1. A dehydration device for pathological tumor sections comprises a base (1) and is characterized in that a vertical cylinder (2) is fixedly installed at the center of the base (1), a rotating shaft (3) capable of rotating is inserted into the center of the cylinder (2), and a torsion spring (4) is installed between the lower end of the rotating shaft (3) and the base (1); the outer edge surface of the barrel body (2) is provided with a helical blade (5), and the barrel body (2) is provided with a helical groove (6) corresponding to the helical blade (5); a sleeve (7) which can only move up and down along the rotating shaft (3) is sleeved on the rotating shaft (3), a cross rod (8) is fixed on the sleeve (7), the outer end of the cross rod (8) penetrates through the spiral groove (6) and is arranged on the outer side of the barrel body (2), a vertical guide rod (9) is inserted in the outer end of the cross rod (8), and the guide rod (9) can move up and down and reset upwards; the upper end of the guide rod (9) is provided with a first permanent magnet (10), and the lower end of the guide rod (9) is fixed with a placing box (11);
soaking pools (12) with upward openings are arranged on the upper side surface of the spiral blade (5) at equal intervals from bottom to top, a timer (13) is fixed at the front end of each soaking pool (12), a plurality of fixing blocks (14) which correspond to the soaking pools (12) one by one are arranged on the barrel body (2), a limiting rod (15) which can move up and down and reset upwards is inserted in each fixing block (14), and the limiting rod (15) can block the cross rod (8) from moving upwards along the spiral groove (6) after moving downwards; a second permanent magnet (16) is fixed at the upper end of the limiting rod (15); a plurality of electromagnets (17) which correspond to the second permanent magnets (16) one by one are fixed on the lower side surface of the helical blade (5); each timer (13) controls the electromagnet (17) right above the timer, and the electromagnet (17) is powered off after the timer (13) returns to zero.
2. The dehydration device for pathological tumor sections according to claim 1, wherein the timer (13) comprises a housing (18), a rotatable pointer (19) and a reset pointer (19) are installed at the center of the housing (18), the upper end of the pointer (19) is wrapped with a first electrode plate (20), and a fan-shaped second electrode plate (21) is installed on the housing (18); the second electrode avoids the zero graduation line right above the timer (13); when the hand (19) is rotated, the first electrode piece (20) and the second electrode piece (21) are in contact with each other, and the electromagnet (17) corresponding to the timer (13) is energized.
3. The dehydration device for pathological sections of tumors according to claim 1, wherein a plurality of wedge blocks (22) between two adjacent electromagnets (17) are uniformly distributed on the lower side of the helical blade (5).
4. The tumor pathological section dehydration device according to claim 1, characterized in that the base (1) is provided with an annular groove (23), and the outer edge of the helical blade (5) is provided with a baffle (24).
5. The dehydration device for pathological tumor sections according to claim 1, wherein the cross section of the rotating shaft (3) is square, and the upper end of the rotating shaft (3) is provided with a knob (25).
6. The dehydration device for pathological tumor sections as claimed in claim 1, wherein the guide rod (9) is sleeved with a first tension spring (26) between the first permanent magnet (10) and the cross rod (8), and the limiting rod (15) is sleeved with a second tension spring (27) between the second permanent magnet (16) and the fixing block (14).
7. The dehydration device of the pathological section of tumor according to claim 1, characterized in that said box (11) is made of stainless steel screen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011057869.1A CN112179737B (en) | 2020-09-30 | 2020-09-30 | Tumour pathological section dewatering device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011057869.1A CN112179737B (en) | 2020-09-30 | 2020-09-30 | Tumour pathological section dewatering device |
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| CN112179737A true CN112179737A (en) | 2021-01-05 |
| CN112179737B CN112179737B (en) | 2023-02-03 |
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| CN202011057869.1A Active CN112179737B (en) | 2020-09-30 | 2020-09-30 | Tumour pathological section dewatering device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113178635A (en) * | 2021-03-31 | 2021-07-27 | 杭州震弘环境科技有限公司 | Waste lithium cobalt oxide battery recovery equipment |
| CN114136742A (en) * | 2021-11-23 | 2022-03-04 | 桂林医学院 | Immunohistochemical automatic mounting device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113178635A (en) * | 2021-03-31 | 2021-07-27 | 杭州震弘环境科技有限公司 | Waste lithium cobalt oxide battery recovery equipment |
| CN114136742A (en) * | 2021-11-23 | 2022-03-04 | 桂林医学院 | Immunohistochemical automatic mounting device |
| CN114136742B (en) * | 2021-11-23 | 2024-03-15 | 桂林医学院 | Automatic sealing device of immunohistochemistry |
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| CN112179737B (en) | 2023-02-03 |
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