CN112159805A - DNA draws uses mixing device - Google Patents
DNA draws uses mixing device Download PDFInfo
- Publication number
- CN112159805A CN112159805A CN202010980187.1A CN202010980187A CN112159805A CN 112159805 A CN112159805 A CN 112159805A CN 202010980187 A CN202010980187 A CN 202010980187A CN 112159805 A CN112159805 A CN 112159805A
- Authority
- CN
- China
- Prior art keywords
- mixing
- box
- belt pulley
- mixing device
- rotating shaft
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
- B01F27/80—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis
- B01F27/85—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis with two or more stirrers on separate shafts
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
- B01F27/80—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis
- B01F27/90—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis with paddles or arms
- B01F27/906—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis with paddles or arms with fixed axis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/50—Movable or transportable mixing devices or plants
- B01F33/501—Movable mixing devices, i.e. readily shifted or displaced from one place to another, e.g. portable during use
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/20—Measuring; Control or regulation
- B01F35/22—Control or regulation
- B01F35/221—Control or regulation of operational parameters, e.g. level of material in the mixer, temperature or pressure
- B01F35/2215—Temperature
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/40—Mounting or supporting mixing devices or receptacles; Clamping or holding arrangements therefor
- B01F35/41—Mounting or supporting stirrer shafts or stirrer units on receptacles
- B01F35/411—Mounting or supporting stirrer shafts or stirrer units on receptacles by supporting only one extremity of the shaft
- B01F35/4111—Mounting or supporting stirrer shafts or stirrer units on receptacles by supporting only one extremity of the shaft at the top of the receptacle
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/90—Heating or cooling systems
- B01F35/92—Heating or cooling systems for heating the outside of the receptacle, e.g. heated jackets or burners
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/90—Heating or cooling systems
- B01F2035/99—Heating
Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a mixing device for DNA extraction, which comprises a first motor, a water pump, a mixing blade, a temperature regulating box and a mixing box, wherein the first motor is used for rotating to drive a first belt pulley to rotate so as to drive a second belt pulley to rotate, and the mixing blade on a stirring rotating shaft is driven to rotate so as to realize the mixing of a solution in the mixing box, the mixing blade rotates together in the rotating process of the mixing blade, so that the effect of mixing the solution in the mixing box is improved, the temperature of the solution in the temperature regulating box is regulated by utilizing a heat conducting medium through a heat conducting pipe, the solution in the temperature regulating box further enters the mixing box through a liquid guiding pipe, the manual mixing of a detector is not needed, the working strength of the detector is greatly reduced, after the mixing is finished, a containing container such as a test tube is placed between a first clamping plate and a second clamping plate, the second clamping plate moves towards the first clamping plate under the action of spring force, thereby the realization holds the clamp of container to holding such as test tube and presss from both sides tightly spacing, makes things convenient for the measurement personnel to carry out DNA and draws.
Description
Technical Field
The invention relates to a blending device, in particular to a blending device for DNA extraction.
Background
DNA extraction is mainly a CTAB method, and other methods are physical methods such as a glass bead method, an ultrasonic method, a grinding method and a freeze-thaw method. Chemical methods such as guanidinium isothiocyanate method, alkali lysis method. The biological mode comprises the following steps: an enzymatic method. According to the nucleic acid separation and purification method, there are silica material, anion exchange resin, etc. The extraction method of genome DNA of different organisms (plants, animals and microorganisms) is different, but mainly comprises the steps of adding amyl alcohol, chloroform and other solutions, and further uniformly mixing.
At present, the manual mixing is generally carried out by depending on detection personnel, the working intensity of the detection personnel is greatly increased, and meanwhile, the mixing effect is difficult to ensure.
Disclosure of Invention
The invention aims to provide a mixing device for DNA extraction, which aims to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a mixing device for DNA extraction comprises a top plate, wherein a mixing box is fixedly mounted at the top of the top plate, a first motor is fixedly mounted at the top of the mixing box through bolts, an output shaft of the first motor extends into the mixing box and is fixedly provided with a first belt pulley, the first belt pulley is a double-belt pulley, a first mixing device and a second mixing device are symmetrically arranged at positions, which are positioned at two sides of the first belt pulley, in the mixing box, the first mixing device comprises a second belt pulley, a stirring rotating shaft, stirring blades and mixing blades, the bottom end of the stirring rotating shaft is rotatably mounted at the bottom of the inner side of the mixing box through a bearing seat, the top end of the stirring rotating shaft is rotatably mounted at the top of the inner side of the mixing box through the bearing seat, the second belt pulley is mounted on the stirring rotating shaft, the first belt pulley is connected with the second belt pulley through a belt, and the, the stirring device is characterized in that rotating shafts are rotated on two sides of the stirring blades, three mixing blades are arranged on the outer side of the rotating shafts at equal intervals along the circumferential direction, impellers are arranged between a first mixing device and a second mixing device below the inner part of the mixing box, the impellers are fixedly arranged on the first rotating shaft, the impellers and the first rotating shaft are concentrically arranged, the bottom end of the first rotating shaft penetrates through the mixing box and a top plate, a first bevel gear is fixedly arranged on the bottom end of the first rotating shaft, through holes for the first rotating shaft to pass through are respectively arranged on corresponding positions on the mixing box and the top plate, the inner wall of each through hole is rotatably connected with the first rotating shaft through bearings, a sealing rubber ring is arranged between the inner wall of each through hole and the first rotating shaft, a second bevel gear is arranged on one side of the first bevel gear, the first bevel gear is meshed with the second bevel gear, the second bevel gear is fixedly, the second motor is fixedly arranged on one side of the mounting plate, the mounting plate is fixedly arranged on the bottom of the top plate, the bottom of the mixing box is symmetrically provided with a first height adjusting device and a second height adjusting device, the liquid outlet of the mixing box is connected with one end of the three-way pipe through a liquid guide pipe, a third valve is arranged on the liquid guide pipe, the second end of the three-way pipe is connected with the liquid inlet of the water pump through the liquid guide pipe, a fifth valve is arranged on the liquid guide pipe, the water pump is fixedly arranged on the bottom of the top plate, the liquid outlet of the water pump is connected with the liquid inlet of the temperature adjusting box through the liquid guide pipe, the temperature adjusting box is fixedly arranged on the top of the mixing box, the liquid outlet of the temperature adjusting box is connected with the liquid inlet of the mixing box through the liquid guide pipe, one side, a first clamping plate and a second clamping plate are arranged below the other end of the catheter.
As a further scheme of the invention: the structure and each part specification of the first blending device and the second blending device are the same.
As a still further scheme of the invention: mixing case top symmetry is provided with first feed hopper and second feed hopper, the discharge gate of first feed hopper passes through the liquid pipe and is connected with the inlet of mixing case, installs first valve on the liquid pipe, the discharge gate of second feed hopper passes through the liquid pipe and is connected with the inlet of mixing case, installs the second valve on the liquid pipe, first feed hopper and the equal threaded connection in second feed hopper outside have sealed lid.
As a still further scheme of the invention: the second height adjusting device comprises a barrel and a rod body, the barrel is fixedly installed at the top of the bottom plate, the rod body is arranged in the barrel in a sliding mode, the top end of the rod body extends out of the barrel and is fixedly installed at the bottom of the mixing box, and a threaded through hole used for being connected with the bolt in a threaded mode is formed in the outer side of the barrel.
As a still further scheme of the invention: the second height adjusting device and the first height adjusting device are identical in structure and specifications of all parts.
As a still further scheme of the invention: the temperature regulating box is internally provided with a heat conducting pipe, two ends of the heat conducting pipe extend out of the temperature regulating box, and the part of the heat conducting pipe, which is positioned in the temperature regulating box, is spirally arranged.
As a still further scheme of the invention: the mixing box front surface is provided with the observation window, the observation window is the toughened glass material.
As a still further scheme of the invention: first splint and second splint are relative setting, first splint and second splint are the arc, first splint fixed mounting is inboard at first riser, first riser fixed mounting is at the bottom plate top, second splint outside fixed mounting has the one end of horizontal pole, the horizontal pole transversely runs through the second riser, second riser fixed mounting is at the bottom plate top, set up the through-hole that is used for the horizontal pole to pass on the second riser, the other end fixedly connected with pull ring of horizontal pole, be provided with the spring between the pull ring and the second riser outside, the spring housing is established in the horizontal pole outside and both ends difference fixed connection in the pull ring and the second riser outside.
As a still further scheme of the invention: and the bottom of the bottom plate is symmetrically provided with traveling wheels.
As a still further scheme of the invention: the first motor, the second motor and the water pump are respectively electrically connected with the switch through wires, and the switch is electrically connected with an external power supply through wires.
Compared with the prior art, the invention has the beneficial effects that:
1. the first motor is utilized to rotate to drive the first belt pulley to rotate, so that the second belt pulley is further driven to rotate, the stirring blade on the stirring rotating shaft is further driven to rotate, the solution in the mixing box is mixed uniformly, the mixing blade rotates together in the rotating process of the stirring blade, the effect of mixing the solution in the mixing box is improved, the mixing effect is good, and the effect of mixing the solution in the mixing box is further improved by arranging the second mixing device;
2. the solution in the mixing box enters the temperature adjusting box through the liquid guide pipe by using the water pump, when the temperature of the solution needs to be adjusted, the temperature of the solution in the temperature adjusting box is adjusted by using the heat conducting medium through the heat conducting pipe, the solution in the temperature adjusting box further enters the mixing box through the liquid guide pipe, the mixing effect is further improved, the manual mixing of detection personnel is not needed, and the working intensity of the detection personnel is greatly reduced;
3. after the mixing is finished, the cross rod is pulled to drive the second clamping plate to move, the containing containers such as test tubes are further placed between the first clamping plate and the second clamping plate, the second clamping plate moves towards the first clamping plate under the action of spring force, so that the containing containers such as test tubes are clamped and limited, and the rubber pads are arranged to improve the clamping effect of the containing containers such as test tubes;
4. thereby set up the walking wheel and make things convenient for the detector thrust unit to remove, the regulation body of rod is flexible in the barrel to the realization utilizes the screw through-hole internal rotation that the bolt was seted up on the barrel to the regulation of mixing incasement height, thereby realizes injecing the extrusion of the body of rod, easy operation makes things convenient for the detector to carry out DNA and draws.
Drawings
FIG. 1 is a schematic view showing the structure of a kneading apparatus for DNA extraction.
FIG. 2 is a front view of the mixing apparatus for DNA extraction.
FIG. 3 is a schematic view of the structure at the position A of the mixing apparatus for DNA extraction.
FIG. 4 is a schematic view of the structure at B in the mixing apparatus for DNA extraction.
FIG. 5 is a schematic view showing the structure of a second holder in the mixing apparatus for DNA extraction.
FIG. 6 is a schematic diagram showing the structure of a temperature-control box in the kneading apparatus for DNA extraction.
FIG. 7 is a schematic view of the structure at position C in the mixing apparatus for DNA extraction.
Shown in the figure: a blending box 1, a first valve 2, a first feeding funnel 3, a sealing cover 4, a temperature adjusting box 5, a first motor 6, a first belt pulley 7, a second belt pulley 8, a second valve 9, a second feeding funnel 10, a stirring rotating shaft 11, a first clamping plate 12, a switch 13, a first blending device 14, a third valve 15, a fourth valve 16, a first vertical plate 17, a fifth valve 18, a first height adjusting device 19, a water pump 20, a bottom plate 21 and a traveling wheel 22, the heat conduction pipe 23, the second height adjusting device 24, the cylinder 25, the rod body 26, the second blending device 27, the observation window 28, the three-way pipe 29, the stirring blade 30, the blending blade 31, the cross rod 32, the spring 33, the pull ring 34, the second vertical plate 35, the second clamping plate 36, the rubber pad 37, the top plate 38, the impeller 39, the second motor 40, the mounting plate 41, the first rotating shaft 42, the first bevel gear 43, the second rotating shaft 44 and the second bevel gear 45.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1 to 7, in an embodiment of the present invention, a DNA extraction mixing device includes a mixing box 1, a first valve 2, a first feeding funnel 3, a sealing cover 4, a temperature-adjusting box 5, a first motor 6, a first belt pulley 7, a second belt pulley 8, a second valve 9, a second feeding funnel 10, a stirring rotating shaft 11, a first clamping plate 12, a switch 13, a first mixing device 14, a third valve 15, a fourth valve 16, a first vertical plate 17, a fifth valve 18, a first height adjusting device 19, a water pump 20, a bottom plate 21, a traveling wheel 22, a heat pipe 23, a second height adjusting device 24, a cylinder 25, a rod body 26, a second mixing device 27, an observation window 28, a three-way pipe 29, a stirring blade 30, a mixing blade 31, a cross bar 32, a spring 33, a pull ring 34, a second vertical plate 35, a second clamping plate 36, a rubber pad 37, and a top plate 38, wherein the top of the mixing box 1 is fixedly installed on the top plate 38, the mixing device is characterized in that a first motor 6 is fixedly installed at the top of the mixing box 1 through a bolt, an output shaft of the first motor 6 extends into the mixing box 1 and is fixedly provided with a first belt pulley 7, the first belt pulley 7 is a double belt pulley, a first mixing device 14 and a second mixing device 27 are symmetrically arranged at positions, located on two sides of the first belt pulley 7, in the mixing box 1, the first mixing device 14 comprises a second belt pulley 8, a stirring rotating shaft 11, a stirring blade 30 and a mixing blade 31, the bottom end of the stirring rotating shaft 11 is rotatably installed at the bottom of the inner side of the mixing box 1 through a bearing seat, the top end of the stirring rotating shaft 11 is rotatably installed at the top of the inner side of the mixing box 1 through the bearing seat, the second belt pulley 8 is installed on the stirring rotating shaft 11, the first belt pulley 7 is connected with the second belt pulley 8 through a belt, and the stirring blade, the stirring device is characterized in that rotating shafts are rotated on two sides of the stirring blade 30, three blending blades 31 are installed on the outer side of the rotating shafts at equal intervals along the circumferential direction, the structures and specifications of the first blending device 14 and the second blending device 27 are the same, an impeller 39 is arranged between the first blending device 14 and the second blending device 27 below the inner part of the blending box 1, the impeller 39 is fixedly installed on a first rotating shaft 42, the impeller 39 and the first rotating shaft 42 are concentrically arranged, the bottom end of the first rotating shaft 42 penetrates through the blending box 1 and a top plate 38 and is fixedly provided with a first bevel gear 43, through holes for the first rotating shaft 42 to pass through are respectively arranged on corresponding positions on the blending box 1 and the top plate 38, the inner wall of each through hole is rotatably connected with the first rotating shaft 42 through a bearing, a sealing rubber ring is arranged between the inner wall of each through hole and the first rotating shaft 42, and a second bevel, the first bevel gear 43 is meshed with the second bevel gear 45, the second bevel gear 45 is fixedly mounted at one end of a second rotating shaft 44, the other end of the second rotating shaft 44 is fixedly connected with an output shaft of a second motor 40 through a coupler, the second motor 40 is fixedly mounted at one side of a mounting plate 41, the mounting plate 41 is fixedly mounted at the bottom of the top plate 38, a first feeding funnel 3 and a second feeding funnel 10 are symmetrically arranged above the top of the mixing box 1, a discharge port of the first feeding funnel 3 is connected with a liquid inlet of the mixing box 1 through a liquid guide pipe, a first valve 2 is mounted on the liquid guide pipe, a discharge port of the second feeding funnel 10 is connected with a liquid inlet of the mixing box 1 through a liquid guide pipe, a second valve 9 is mounted on the liquid guide pipe, sealing covers 4 are in threaded connection with the outer sides of the first feeding funnel 3 and the second feeding funnel 10, a first height adjusting device 19 and a second height adjusting device 24 are symmetrically arranged, the second height adjusting device 24 comprises a barrel 25 and a rod body 26, the barrel 25 is fixedly installed at the top of the bottom plate 21, the rod body 26 is arranged in the barrel 25 in a sliding mode, the top end of the rod body 26 extends out of the barrel 25 and is fixedly installed at the bottom of the blending box 1, a threaded through hole for being in threaded connection with a bolt is formed in the outer side of the barrel 25, the structures and specifications of all parts of the second height adjusting device 24 and the first height adjusting device 19 are the same, traveling wheels 22 are symmetrically installed at the bottom of the bottom plate 21, a liquid outlet of the blending box 1 is connected with one end of a three-way pipe 29 through a liquid guide pipe, a third valve 15 is installed on the liquid guide pipe, the second end of the three-way pipe 29 is connected with a liquid inlet of the water pump 20 through the liquid guide pipe, a fifth valve 18 is installed on the liquid guide pipe, the water pump 20, temperature adjusting box 5 fixed mounting is at mixing box 1 top, the liquid outlet of temperature adjusting box 5 passes through the catheter and is connected with the inlet of mixing box 1, be provided with heat pipe 23 in the temperature adjusting box 5, temperature adjusting box 5 all stretches out at the both ends of heat pipe 23, and the part that heat pipe 23 is located temperature adjusting box 5 is for spiraling the setting, mixing box 1 one side fixed mounting has switch 13, mixing box 1 front surface is provided with observation window 28, observation window 28 is the toughened glass material, the third end of three-way pipe 29 is connected with the one end of catheter, installs fourth valve 16 on the catheter, and the other end below of catheter is provided with first splint 12 and second splint 36, first splint 12 and second splint 36 are relative setting, first splint 12 and second splint 36 are the arc, first splint 12 fixed mounting is inboard at first riser 17, first riser 17 fixed mounting is at bottom plate 21 top, 36 outside fixed mounting of second splint has the one end of horizontal pole 32, horizontal pole 32 transversely runs through second riser 35, second riser 35 fixed mounting is at bottom plate 21 top, offer the through-hole that is used for horizontal pole 32 to pass on the second riser 35, the other end fixedly connected with pull ring 34 of horizontal pole 32, be provided with spring 33 between the pull ring 34 and the second riser 35 outside, spring 33 cover is established in the horizontal pole 32 outside and both ends difference fixed connection in the pull ring 34 and the second riser 35 outside, first motor 6, second motor 40 and water pump 20 are connected through wire and switch 13 electricity respectively, and switch 13 passes through the wire and is connected with external power supply electricity.
The working principle of the invention is as follows:
when DNA needs to be extracted, firstly, the sealing cover 4 is opened, DNA of animals and plants and microorganisms and solutions of isoamylol, amyl alcohol, chloroform and the like are further poured into the blending box 1 through the first feeding funnel 3 and the second feeding funnel 10 respectively, the first motor 6 is further opened by the switch 13, the first motor 6 is rotated to drive the first belt pulley 7 to rotate, so that the second belt pulley 8 is further driven to rotate, the stirring blade 30 on the stirring rotating shaft 11 is further driven to rotate, the blending of the solution in the blending box 1 is realized, the blending blade 31 rotates together in the rotating process of the stirring blade 30, the effect of blending the solution in the blending box 1 is improved, the blending effect is good, the second blending device 27 is arranged to further improve the effect of blending the solution in the blending box 1, the switch 13 is used for opening the water pump 20, the third valve 15 is opened, the solution in the blending box 1 enters the temperature adjusting box 5 through the liquid guide pipe by using the water pump 20, when the temperature of the solution needs to be adjusted, the heat conducting medium passes through the heat conducting pipe 23, so that the temperature of the solution in the temperature adjusting box 5 is adjusted, the solution in the temperature adjusting box 5 further enters the blending box through the liquid guide pipe, the blending effect is further improved, manual blending by detection personnel is not needed, the working strength of the detection personnel is greatly reduced, after the blending is completed, the cross rod 32 is pulled to drive the second clamping plate 36 to move, the containing containers such as test tubes are further placed between the first clamping plate 12 and the second clamping plate 36, the second clamping plate 36 moves towards the first clamping plate 12 under the action of the elastic force of the spring 33, so that the clamping limit of the containing containers such as test tubes is realized, the clamping effect of the containing containers such as test tubes is improved by arranging the rubber pad 37, the fifth valve 18 is further closed and the fourth valve, thereby make the solution after the even completion of mixing in mixing box 1 pass through the catheter and discharge, thereby set up walking wheel 22 and make things convenient for measurement personnel thrust unit to remove, adjust the body of rod 26 and stretch out and draw back in barrel 25 to the realization is to the regulation of mixing box 1 internal height, utilizes the screw through-hole internal rotation that the bolt was seted up on barrel 25, thereby realizes prescribing a limit to the extrusion of the body of rod 26, and easy operation makes things convenient for measurement personnel to carry out DNA and draws.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents and modifications of some features of the invention can be made without departing from the spirit and scope of the invention.
Claims (10)
1. The utility model provides a DNA draws and uses mixing device, includes roof (38), its characterized in that: the top of the top plate (38) is fixedly provided with a mixing box (1), the top of the mixing box (1) is fixedly provided with a first motor (6) through a bolt, an output shaft of the first motor (6) extends into the mixing box (1) and is fixedly provided with a first belt pulley (7), the first belt pulley (7) is a double-belt pulley, a first mixing device (14) and a second mixing device (27) are symmetrically arranged at positions, which are positioned at two sides of the first belt pulley (7), in the mixing box (1), the first mixing device (14) comprises a second belt pulley (8), a mixing rotating shaft (11), a mixing blade (30) and a mixing blade (31), the bottom end of the mixing rotating shaft (11) is rotatably arranged at the inner bottom of the mixing box (1) through a bearing seat, the top end of the mixing rotating shaft (11) is rotatably arranged at the inner top of the mixing box (1) through the bearing seat, install second belt pulley (8) on stirring pivot (11), first belt pulley (7) are connected with second belt pulley (8) through the belt, install stirring vane (30) on stirring pivot (11), stirring vane (30) both sides are rotated the pivot, and three mixing blade (31) are installed along the circumferencial direction equidistance in the pivot outside, be provided with impeller (39) between mixing tank (1) inside below first mixing device (14) and second mixing device (27), impeller (39) fixed mounting is on first pivot (42), impeller (39) and first pivot (42) are concentric setting, mixing tank (1) and roof (38) and fixed mounting are run through to the bottom of first pivot (42) have first bevel gear (43), set up a through-hole that is used for first pivot (42) to pass on mixing tank (1) and roof (38) respectively on corresponding the position, the through hole inner wall is rotatably connected with a first rotating shaft (42) through a bearing, a sealing rubber ring is arranged between the through hole inner wall and the first rotating shaft (42), a second bevel gear (45) is arranged on one side of the first bevel gear (43), the first bevel gear (43) is meshed with the second bevel gear (45), the second bevel gear (45) is fixedly arranged at one end of a second rotating shaft (44), the other end of the second rotating shaft (44) is fixedly connected with an output shaft of a second motor (40) through a coupler, the second motor (40) is fixedly arranged on one side of a mounting plate (41), the mounting plate (41) is fixedly arranged at the bottom of a top plate (38), a first height adjusting device (19) and a second height adjusting device (24) are symmetrically arranged at the bottom of the blending box (1), a liquid outlet of the blending box (1) is connected with one end of a three-way pipe (29) through a liquid, install third valve (15) on the catheter, the second end of three-way pipe (29) is connected through the inlet of catheter and water pump (20), installs fifth valve (18) on the catheter, water pump (20) fixed mounting is in roof (38) bottom, the liquid outlet of water pump (20) passes through the catheter and is connected with the inlet of temperature regulating box (5), and temperature regulating box (5) fixed mounting is at mixing box (1) top, the liquid outlet of temperature regulating box (5) passes through the catheter and is connected with the inlet of mixing box (1), mixing box (1) one side fixed mounting has switch (13), the third end of three-way pipe (29) is connected with the one end of catheter, installs fourth valve (16) on the catheter, and the other end below of catheter is provided with first splint (12) and second splint (36).
2. The mixing device for DNA extraction according to claim 1, characterized in that: the structure and the specification of each part of the first blending device (14) and the second blending device (27) are the same.
3. The mixing device for DNA extraction according to claim 1, characterized in that: mixing case (1) top symmetry is provided with first feed hopper (3) and second feed hopper (10), the discharge gate of first feed hopper (3) passes through the liquid pipe and is connected with the inlet of mixing case (1), installs first valve (2) on the liquid pipe, the discharge gate of second feed hopper (10) passes through the liquid pipe and is connected with the inlet of mixing case (1), installs second valve (9) on the liquid pipe, the equal threaded connection in first feed hopper (3) and second feed hopper (10) outside has sealed lid (4).
4. The mixing device for DNA extraction according to claim 1, characterized in that: second height adjusting device (24) include barrel (25) and the body of rod (26), barrel (25) fixed mounting is at bottom plate (21) top, it is provided with the body of rod (26) to slide in barrel (25), barrel (25) and fixed mounting are stretched out on the top of the body of rod (26) in mixing case (1) bottom, the screw thread through-hole that is used for with bolt threaded connection is offered in barrel (25) outside.
5. The mixing device according to claim 1 or 4, wherein: the second height adjusting device (24) and the first height adjusting device (19) are identical in structure and specifications of all components.
6. The mixing device for DNA extraction according to claim 1, characterized in that: be provided with heat pipe (23) in thermoregulation box (5), thermoregulation box (5) all stretches out at the both ends of heat pipe (23), and the part that heat pipe (23) are located thermoregulation box (5) is for circling the setting.
7. The mixing device for DNA extraction according to claim 1, characterized in that: the mixing box (1) front surface is provided with observation window (28), observation window (28) are toughened glass material.
8. The mixing device for DNA extraction according to claim 1, characterized in that: the first clamping plate (12) and the second clamping plate (36) are arranged oppositely, the first clamping plate (12) and the second clamping plate (36) are both arc-shaped plates, the first splint (12) is fixedly arranged at the inner side of a first vertical plate (17), the first vertical plate (17) is fixedly arranged at the top of the bottom plate (21), one end of a cross bar (32) is fixedly arranged on the outer side of the second clamping plate (36), the cross bar (32) transversely penetrates through the second vertical plate (35), the second vertical plate (35) is fixedly arranged at the top of the bottom plate (21), a through hole for the cross rod (32) to pass through is formed in the second vertical plate (35), the other end of the cross bar (32) is fixedly connected with a pull ring (34), a spring (33) is arranged between the pull ring (34) and the outer side of the second vertical plate (35), the spring (33) is sleeved on the outer side of the cross rod (32) and two ends of the spring are respectively and fixedly connected to the outer sides of the pull ring (34) and the second vertical plate (35).
9. The mixing device for DNA extraction according to claim 8, wherein: and the bottom of the bottom plate (21) is symmetrically provided with traveling wheels (22).
10. The mixing device for DNA extraction according to claim 1, characterized in that: the first motor (6), the second motor (40) and the water pump (20) are electrically connected with the switch (13) through wires respectively, and the switch (13) is electrically connected with an external power supply through wires.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010980187.1A CN112159805B (en) | 2020-09-17 | 2020-09-17 | DNA draws uses mixing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010980187.1A CN112159805B (en) | 2020-09-17 | 2020-09-17 | DNA draws uses mixing device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112159805A true CN112159805A (en) | 2021-01-01 |
| CN112159805B CN112159805B (en) | 2022-07-01 |
Family
ID=73858292
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010980187.1A Active CN112159805B (en) | 2020-09-17 | 2020-09-17 | DNA draws uses mixing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112159805B (en) |
Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205361340U (en) * | 2016-01-29 | 2016-07-06 | 南通汉瑞新材料科技有限公司 | Chemical production is with reaction unit who has sample function |
| CN205603472U (en) * | 2016-03-11 | 2016-09-28 | 成都绿迪科技有限公司 | Novel fertilizer fermentation device |
| CN205659586U (en) * | 2016-06-06 | 2016-10-26 | 潍坊市园林管理处 | Safflower maple cuttage agitating unit for matrix |
| CN206215065U (en) * | 2016-10-27 | 2017-06-06 | 天津联森包装制品有限公司 | Corrugated board production glue making machine |
| CN107029596A (en) * | 2017-06-16 | 2017-08-11 | 洛阳宁竹药业有限公司 | It is a kind of that there is cleaning and the pharmacy material-compound tank of height adjusting function |
| CN206577685U (en) * | 2017-03-22 | 2017-10-24 | 自贡中电辰光新材料有限公司 | A kind of high efficient mixer for polyflon raw material |
| CN108068715A (en) * | 2016-11-10 | 2018-05-25 | 江苏明雨耐磨材料有限公司 | A kind of pilotless automobile camera bracket with bumper and absorbing shock |
| CN108187093A (en) * | 2018-01-12 | 2018-06-22 | 邹百川 | It is a kind of to carry out environmental protecting sterilized method using formic acid and calcium lignosulfonate mixed liquid |
| CN208375502U (en) * | 2018-06-23 | 2019-01-15 | 福建省将乐好家园木业有限公司 | Clamping device is used in a kind of processing of bamboo and wood products |
| CN111068564A (en) * | 2019-09-26 | 2020-04-28 | 杭州为我健康新零售科技有限公司 | Honey mixing system and honey mixing method suitable for honey beverage vending machine |
| CN111572958A (en) * | 2020-05-21 | 2020-08-25 | 青岛浩丰食品集团有限公司 | A intelligent flute case for processing storage of modern agriculture fruit vegetables |
| CN111592189A (en) * | 2020-05-29 | 2020-08-28 | 刘洪起 | Integrated sewage treatment equipment and use method thereof |
| CN211386617U (en) * | 2019-12-12 | 2020-09-01 | 重庆交通大学 | Magnesium alloy single-point incremental forming fixing tool |
-
2020
- 2020-09-17 CN CN202010980187.1A patent/CN112159805B/en active Active
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205361340U (en) * | 2016-01-29 | 2016-07-06 | 南通汉瑞新材料科技有限公司 | Chemical production is with reaction unit who has sample function |
| CN205603472U (en) * | 2016-03-11 | 2016-09-28 | 成都绿迪科技有限公司 | Novel fertilizer fermentation device |
| CN205659586U (en) * | 2016-06-06 | 2016-10-26 | 潍坊市园林管理处 | Safflower maple cuttage agitating unit for matrix |
| CN206215065U (en) * | 2016-10-27 | 2017-06-06 | 天津联森包装制品有限公司 | Corrugated board production glue making machine |
| CN108068715A (en) * | 2016-11-10 | 2018-05-25 | 江苏明雨耐磨材料有限公司 | A kind of pilotless automobile camera bracket with bumper and absorbing shock |
| CN206577685U (en) * | 2017-03-22 | 2017-10-24 | 自贡中电辰光新材料有限公司 | A kind of high efficient mixer for polyflon raw material |
| CN107029596A (en) * | 2017-06-16 | 2017-08-11 | 洛阳宁竹药业有限公司 | It is a kind of that there is cleaning and the pharmacy material-compound tank of height adjusting function |
| CN108187093A (en) * | 2018-01-12 | 2018-06-22 | 邹百川 | It is a kind of to carry out environmental protecting sterilized method using formic acid and calcium lignosulfonate mixed liquid |
| CN208375502U (en) * | 2018-06-23 | 2019-01-15 | 福建省将乐好家园木业有限公司 | Clamping device is used in a kind of processing of bamboo and wood products |
| CN111068564A (en) * | 2019-09-26 | 2020-04-28 | 杭州为我健康新零售科技有限公司 | Honey mixing system and honey mixing method suitable for honey beverage vending machine |
| CN211386617U (en) * | 2019-12-12 | 2020-09-01 | 重庆交通大学 | Magnesium alloy single-point incremental forming fixing tool |
| CN111572958A (en) * | 2020-05-21 | 2020-08-25 | 青岛浩丰食品集团有限公司 | A intelligent flute case for processing storage of modern agriculture fruit vegetables |
| CN111592189A (en) * | 2020-05-29 | 2020-08-28 | 刘洪起 | Integrated sewage treatment equipment and use method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112159805B (en) | 2022-07-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107983288A (en) | On-line monitoring intelligent linkage reaction kettle | |
| CN112159805B (en) | DNA draws uses mixing device | |
| CN212586093U (en) | Micro-fluidic chip drug testing sample splitter | |
| CN111672379A (en) | Homogeneity device is used in food deep-processing | |
| CN210303459U (en) | Vacuum stirring device | |
| CN114806860A (en) | Vacuum decompression fermentation system that probiotic fermentation used | |
| CN112387163A (en) | Pharmacy is with batching jar | |
| CN211246200U (en) | Treating agent agitator tank | |
| CN211586611U (en) | Neutralization reaction kettle | |
| CN113913279A (en) | Energy-saving fermentation tank with fine defoaming function for fermentation engineering | |
| CN214514002U (en) | Traditional chinese medicine mycoplasm enzymolysis mixes and cools off all-in-one | |
| CN110354786A (en) | A kind of water treatment agent process units with organic exhaust gas detection function | |
| CN205127963U (en) | Polymer polymerization jar | |
| CN219186652U (en) | High efficiency mixer | |
| CN217418775U (en) | Biological oxidation tank for gold ore treatment | |
| CN221028387U (en) | Microorganism fermentation device | |
| CN205347183U (en) | Mud agitator tank body | |
| CN209242744U (en) | A kind of sewage treatment equipment of synthetic rubber production | |
| CN214233615U (en) | Controllable chemical reagent mixing apparatus | |
| CN211199228U (en) | Tissue cell dissociation device with constant temperature device | |
| CN221108179U (en) | Mixing reaction tank convenient for dosing | |
| CN217746614U (en) | Water purification is with stirring mixing mechanism for water treatment | |
| CN220467948U (en) | Processing equipment capable of uniformly heating | |
| CN218404153U (en) | Medicine raw material fermentation device | |
| CN220861197U (en) | Catalyst preparation device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |