CN112147242A - Fingerprint spectrum construction method for semi-finished product and finished product preparation of Zhenqi Fuzheng capsule - Google Patents
Fingerprint spectrum construction method for semi-finished product and finished product preparation of Zhenqi Fuzheng capsule Download PDFInfo
- Publication number
- CN112147242A CN112147242A CN202010820383.2A CN202010820383A CN112147242A CN 112147242 A CN112147242 A CN 112147242A CN 202010820383 A CN202010820383 A CN 202010820383A CN 112147242 A CN112147242 A CN 112147242A
- Authority
- CN
- China
- Prior art keywords
- fingerprint
- capsule
- preparation
- semi
- finished
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000002775 capsule Substances 0.000 title claims abstract description 146
- 238000002360 preparation method Methods 0.000 title claims abstract description 144
- 238000001228 spectrum Methods 0.000 title claims abstract description 51
- 239000000047 product Substances 0.000 title claims abstract description 44
- 239000011265 semifinished product Substances 0.000 title claims abstract description 25
- 238000010276 construction Methods 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 claims abstract description 41
- 239000003814 drug Substances 0.000 claims abstract description 38
- 241000830535 Ligustrum lucidum Species 0.000 claims abstract description 32
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 28
- 239000012085 test solution Substances 0.000 claims abstract description 23
- 239000007788 liquid Substances 0.000 claims abstract description 16
- 230000004044 response Effects 0.000 claims abstract description 10
- 230000008569 process Effects 0.000 claims abstract description 4
- 238000012545 processing Methods 0.000 claims abstract description 4
- 238000005728 strengthening Methods 0.000 claims description 32
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 30
- 235000006533 astragalus Nutrition 0.000 claims description 29
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 29
- 238000005406 washing Methods 0.000 claims description 28
- 241001061264 Astragalus Species 0.000 claims description 25
- 210000004233 talus Anatomy 0.000 claims description 25
- 238000005303 weighing Methods 0.000 claims description 21
- 238000001514 detection method Methods 0.000 claims description 14
- 238000011068 loading method Methods 0.000 claims description 14
- 238000004128 high performance liquid chromatography Methods 0.000 claims description 13
- 238000002390 rotary evaporation Methods 0.000 claims description 12
- 239000000945 filler Substances 0.000 claims description 11
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical group CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 10
- 238000011156 evaluation Methods 0.000 claims description 10
- 239000011347 resin Substances 0.000 claims description 10
- 229920005989 resin Polymers 0.000 claims description 10
- 239000000706 filtrate Substances 0.000 claims description 9
- 238000009210 therapy by ultrasound Methods 0.000 claims description 9
- 238000010828 elution Methods 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 6
- 239000003480 eluent Substances 0.000 claims description 5
- 238000012856 packing Methods 0.000 claims description 3
- 238000002604 ultrasonography Methods 0.000 claims description 2
- 238000003908 quality control method Methods 0.000 abstract description 17
- 238000005516 engineering process Methods 0.000 abstract description 11
- 238000011160 research Methods 0.000 abstract description 11
- 238000004519 manufacturing process Methods 0.000 abstract description 8
- 229940079593 drug Drugs 0.000 abstract description 2
- 229940107666 astragalus root Drugs 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 39
- 238000012795 verification Methods 0.000 description 17
- 238000011835 investigation Methods 0.000 description 16
- 230000014759 maintenance of location Effects 0.000 description 16
- 238000004587 chromatography analysis Methods 0.000 description 12
- 238000002474 experimental method Methods 0.000 description 10
- 239000000463 material Substances 0.000 description 10
- 239000000843 powder Substances 0.000 description 7
- 239000012488 sample solution Substances 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- 238000007621 cluster analysis Methods 0.000 description 6
- 150000001875 compounds Chemical class 0.000 description 6
- 239000000284 extract Substances 0.000 description 6
- 229940126680 traditional chinese medicines Drugs 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
- 238000011049 filling Methods 0.000 description 5
- 241000045403 Astragalus propinquus Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000004440 column chromatography Methods 0.000 description 3
- 235000008216 herbs Nutrition 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 238000007689 inspection Methods 0.000 description 3
- 238000013441 quality evaluation Methods 0.000 description 3
- 239000004952 Polyamide Substances 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000005485 electric heating Methods 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 229920002647 polyamide Polymers 0.000 description 2
- 238000004445 quantitative analysis Methods 0.000 description 2
- 239000000741 silica gel Substances 0.000 description 2
- 229910002027 silica gel Inorganic materials 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 229910021642 ultra pure water Inorganic materials 0.000 description 2
- 239000012498 ultrapure water Substances 0.000 description 2
- SMDOOINVMJSDPS-UHFFFAOYSA-N Astragaloside Natural products C1=C(O)C(OC)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)OC2C(C(OC3C(C(O)C(O)C(CO)O3)O)C(O)C(CO)O2)O)=C1 SMDOOINVMJSDPS-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- QMNWISYXSJWHRY-XWJCTJPOSA-N astragaloside Chemical compound O1[C@H](C(C)(O)C)CC[C@]1(C)[C@@H]1[C@@]2(C)CC[C@]34C[C@]4(CC[C@H](O[C@H]4[C@@H]([C@@H](O)[C@H](O)CO4)O)C4(C)C)C4[C@@H](O[C@H]4[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O4)O)CC3[C@]2(C)C[C@@H]1O QMNWISYXSJWHRY-XWJCTJPOSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000002980 postoperative effect Effects 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000009777 vacuum freeze-drying Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/86—Signal analysis
- G01N30/8624—Detection of slopes or peaks; baseline correction
- G01N30/8631—Peaks
- G01N30/8634—Peak quality criteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/86—Signal analysis
- G01N30/8675—Evaluation, i.e. decoding of the signal into analytical information
- G01N30/8686—Fingerprinting, e.g. without prior knowledge of the sample components
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/86—Signal analysis
- G01N30/8696—Details of Software
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Quality & Reliability (AREA)
- Library & Information Science (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention provides a fingerprint construction method of a semi-finished product and a finished product preparation of a Zhenqi Fuzheng capsule, which comprises the following steps: (1) preparing a test solution; (2) injecting the test solution obtained in the step (1) into a high performance liquid chromatograph, and recording a chromatogram; (3) processing the chromatographic information in the step (2) by using fingerprint similarity evaluation software to obtain a fingerprint; (4) and (4) selecting a common chromatographic peak with a high response value from the fingerprint obtained in the step (3) as a characteristic fingerprint peak to form a characteristic spectrum. The invention takes the glossy privet fruit and astragalus root capsules as research objects, develops the whole-process quality control research of semi-finished products and finished product preparations, and carries out research comparison on process key link samples in different production stages, thereby realizing the whole-process quality control of medicines and ensuring the quality stability. Compared with the traditional quality control technology, the invention provides a more scientific medicine quality control method and concept, and forms a more scientific quality control system of the Zhenqi Fuzheng capsule.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicine fingerprints, in particular to a fingerprint construction method of a semi-finished product and a finished product preparation of a Zhenqi Fuzheng capsule.
Background
The traditional Chinese medicine fingerprint spectrum technology is a comprehensive quantifiable chemical identification means, wherein a certain traditional Chinese medicine or a traditional Chinese medicine is properly processed, and the information of the chemical components of the traditional Chinese medicine or the traditional Chinese medicine is represented and described by using a modern analysis technology, so that a chromatogram or a spectrogram capable of marking the characteristics of the traditional Chinese medicine or the traditional Chinese medicine is obtained. The traditional Chinese medicine fingerprint can reveal various index components in the traditional Chinese medicine, identify the authenticity of the medicinal materials, evaluate the consistency and stability of the quality of the medicine and the like, and is concerned by people. In recent years, the quality control technology of traditional Chinese medicines is continuously mature, the traditional Chinese medicines are developed to the whole quality evaluation stage from the original quantitative analysis of chemical components, and the fingerprint technology plays a very important role in the quality evaluation process of the traditional Chinese medicines. At present, the traditional Chinese medicine fingerprint spectrum technology has become an international consensus as a quality control method of traditional Chinese medicines, and plays an increasingly important role in the production and quality inspection processes of the traditional Chinese medicines. The efficacy of Chinese herbs is the result of the combined action of many chemical components. However, the existing method for controlling the quality of the traditional Chinese medicine by Chinese pharmacopoeia only measures the content of single or a few known components in the medicine to judge the quality of the medicine, and does not meet the requirements of the traditional Chinese medicine theory. With the continuous progress of science and technology, the quality control of Chinese herbs has been developed from the early stage of limiting and simply describing only single ingredients to the comprehensive and three-dimensional stage of controlling the quality of Chinese herbs.
The Zhenqi Fuzheng Capsule is an immune function enhancer screened by Sunyansi academy and the like, is collected in the drug Standard of the Ministry of health, is a traditional Chinese medicine compound capsule preparation prepared by mixing two traditional Chinese medicines, namely astragalus and glossy privet fruit, according to a certain proportion and extracting and processing, is a traditional Chinese medicine originated in China for assisting cancer treatment, and is often used for treatment such as immunity enhancement, postoperative normal function recovery of organisms and the like. At present, the existing quality standard of the Zhenqi Fuzheng Capsule only carries out qualitative and quantitative analysis on astragaloside contained in the astragalus medicinal material in the compound, but the traditional Chinese medicine compound has complex components, only studies on single components or characteristic components, and cannot comprehensively control the medicine quality.
Disclosure of Invention
In order to solve the problems, the invention takes the semi-finished product and the finished product preparation of the Zhenqi Fuzheng capsule as research objects, adopts the high performance liquid chromatography technology to carry out the research of the fingerprint measuring method, verifies the fingerprint according to the relevant guiding principle of the fingerprint in the Chinese pharmacopoeia, and adopts fingerprint evaluation software to establish the fingerprint of the Zhenqi Fuzheng capsule and construct the characteristic spectrum of the Zhenqi Fuzheng capsule.
In order to achieve the above purpose, the technical scheme of the invention is as follows:
the fingerprint construction method of the semi-finished product and the finished product preparation of the Zhenqi Fuzheng capsule comprises the following steps:
(1) preparing a test solution: weighing the semi-finished preparation or finished preparation of the Zhenqi Fuzheng capsule, adding pure water, dissolving with ultrasound, loading on a chromatographic column, washing with pure water, washing the chromatographic column with ethanol, collecting filtrate, and concentrating by rotary evaporation to obtain a test solution;
(2) and (3) high performance liquid chromatography detection: injecting the test solution obtained in the step (1) into a high performance liquid chromatograph, and recording a chromatogram;
(3) constructing a fingerprint spectrum: processing the chromatographic information of the semi-finished preparation or finished preparation of the glossy privet fruit and astragalus strengthening capsules of a plurality of batches by adopting fingerprint similarity evaluation software to obtain fingerprints of the semi-finished preparation or finished preparation of the glossy privet fruit and astragalus strengthening capsules;
(4) constructing a characteristic spectrum: and (4) selecting a common chromatographic peak with a high response value from the fingerprint obtained in the step (3) as a characteristic fingerprint peak to form a characteristic spectrum.
The fingerprint construction method of the semi-finished product and finished product preparation of the glossy privet fruit and astragalus strengthening capsules comprises the following steps of (1) preparing a test solution: accurately taking 0.5-1.5g of the semi-finished preparation or finished preparation of the Zhenqi Fuzheng capsule, adding 20mL of pure water, performing ultrasonic treatment for 5min until the mixture is completely dissolved, loading the washed chromatographic column, washing the chromatographic column with 1000mL of pure water, washing the chromatographic column with 50-200mL of ethanol, collecting the filtrate, and performing rotary evaporation and concentration to 10mL to obtain a test solution.
The fingerprint construction method of the semi-finished product of the Zhenqi Fuzheng capsule and the finished product preparation comprises the steps of weighing 1.0g of the semi-finished product of the Zhenqi Fuzheng capsule or the finished product preparation; the chromatographic column filler is macroporous resin; the height of the chromatographic column packing is 12 cm; the volume of ethanol eluent was 100 mL.
The fingerprint construction method of the semi-finished product and the finished product preparation of the glossy privet fruit and astragalus strengthening capsules comprises the following steps of (2) carrying out high performance liquid chromatography under the following conditions: using a C18 chromatographic column; the mobile phase A is water, the mobile phase B is acetonitrile, and gradient elution is carried out; the volume flow is 1.0 mL/min; the column temperature is 35 ℃; the detection wavelength is 210nm-285 nm; the amount of the sample was 10. mu.L.
The fingerprint construction method of the semi-finished product and finished product preparation of the Zhenqi Fuzheng capsule comprises the following steps of (2): 0-15min, 5% -10% B; 15-35min, 10% -15% B; 35-60min, 15% -20% B; 60-100min, 20% -25% B; 100-120min, 25-40% B; 120 ℃ for 130min, 40-45% of B; 130-140min, 45-90% B; 140 ℃ for 150min, 90% B; the detection wavelength is 254 nm.
According to the fingerprint construction method of the semi-finished product and finished product preparation of the Zhenqi Fuzheng capsule, in the step (3), software of a traditional Chinese medicine chromatogram fingerprint similarity evaluation system 2004A is adopted, a certain batch of chromatograms are taken as reference spectra, a reference spectrum is generated according to an average value, a time window is set to be 0.05min, and the fingerprints are generated after automatic matching.
The fingerprint spectrum of the semi-finished preparation of the Zhenqi Fuzheng capsule obtained by any one of the methods is disclosed.
The fingerprint spectrum of the finished product preparation of the Zhenqi Fuzheng capsule obtained by any one of the methods.
The semi-finished preparation of the Zhenqi Fuzheng capsule obtained by any one of the methods has a characteristic map, and the map has 12 characteristic fingerprint peaks.
The finished product preparation of the Zhenqi Fuzheng capsule obtained by any one of the methods has a characteristic spectrum, and the spectrum has 28 characteristic fingerprint peaks.
Compared with the prior art, the invention has the beneficial effects that:
the invention adopts a high performance liquid fingerprint chromatogram determination method to systematically construct the fingerprint chromatogram of the Zhenqi Fuzheng Capsule, carries out the whole-process quality control research from the semi-finished preparation of the Zhenqi Fuzheng Capsule, carries out research and comparison on the key links of the production process at different production stages on the sample of the Zhenqi Fuzheng Capsule, realizes the whole-process quality control of the medicine, and ensures the stability of the sample quality. Compared with the traditional quality control technology, the invention provides a relatively scientific medicine quality control method and medicine quality control concept, forms a relatively scientific quality control system of the Zhenqi Fuzheng Capsule by means of the fingerprint technology, and provides a reference for perfecting the quality evaluation of finished preparation of the Zhenqi Fuzheng Capsule. Meanwhile, necessary guarantee is provided for Chinese medicaments entering the international market, and reference basis is provided for the fingerprint technology to become the international standard of Chinese medicament quality control.
Drawings
FIG. 1 is a chromatogram comparison of a semi-finished preparation of Zhenqi Fuzheng Capsule when different fillers are used for a chromatographic column;
FIG. 2 is a chromatogram comparison of the semi-finished preparation of Zhenqi Fuzheng Capsule when the sample loading amount of the chromatographic column is different;
FIG. 3 is a chromatogram comparison of a semi-finished preparation of Zhenqi Fuzheng Capsule when the volumes of eluents of chromatographic columns are different;
FIG. 4 is a chromatogram comparison of a semi-finished preparation of Zhenqi Fuzheng Capsule when the amount (height) of the filler in the chromatographic column is different;
FIG. 5 is a chromatogram comparison of the semi-finished preparation of Zhenqi Fuzheng Capsule at different detection wavelengths;
FIG. 6 shows the result of the stability investigation of the methodological verification of the semifinished glossy privet fruit-astragalus body resistance strengthening capsule preparation;
FIG. 7 shows the result of the methodology verification-precision investigation of the semi-finished preparation of Zhenqi Fuzheng Capsule;
FIG. 8 shows the methodological verification-repeatability investigation result of the Zhenqi Fuzheng capsule semi-finished product;
FIG. 9 is fingerprint of the semi-finished preparation of ZHENQIFUZHENG Capsule;
FIG. 10 is a characteristic spectrum of a semi-finished preparation of Zhenqi Fuzheng Capsule;
FIG. 11 is a cluster analysis of the semi-finished preparation of Zhenqi Fuzheng Capsule;
fig. 12 shows the result of the stability investigation of the finished preparation of zhenqi fuzheng capsule;
fig. 13 shows the result of the methodology verification-precision investigation of the finished preparation of zhenqi fuzheng capsule;
fig. 14 is a methodology verification-repeatability investigation result of the finished product preparation of zhenqi fuzheng capsule;
FIG. 15 is fingerprint of the finished product of Zhenqi Fuzheng Capsule;
FIG. 16 is a characteristic spectrum of the finished preparation of Zhenqi Fuzheng Capsule;
fig. 17 is a cluster analysis of the finished product preparation of zhenqi fuzheng capsule;
fig. 18 is a spectrogram comparison of Zhenqi Fuzheng Capsule in different production stages.
Detailed Description
Materials and reagents used in this example:
a semi-finished preparation of Zhenqi Fuzheng Capsule, with batches S1-S13, wherein the batch S1 is: 20191109, 50 g/batch, collected from Xinbang pharmaceutical Co., Ltd, Guizhou;
13 batches of finished product preparation samples of the Zhenqifuzheng capsule, 50 g/batch, are collected from Xinbang pharmaceutical Co., Ltd.
(1) preparing a test solution: accurately weighing 1.0g of a semi-finished preparation of the Zhenqi Fuzheng capsule, adding 20mL of pure water, carrying out ultrasonic treatment for 5min until the semi-finished preparation is fully dissolved, loading a macroporous resin chromatographic column with the column height of 12cm, weighing 1000mL of pure water for washing, then washing the chromatographic column with 100mL of ethanol, collecting filtrate, and carrying out rotary evaporation and concentration to 10mL to obtain a test solution of the semi-finished preparation of the Zhenqi Fuzheng capsule;
(2) and (3) high performance liquid chromatography detection: injecting the test solution of the semi-finished preparation of the Zhenqi Fuzheng capsule obtained in the step (1) into a high performance liquid chromatograph, and recording a chromatogram; chromatographic conditions are as follows: an Agilent Eclipse XDB-C18 (4.6X 250mm, 5 μm) chromatography column; gradient elution with water (a) -acetonitrile (B) as mobile phase: 0-15min, 5% -10% B; 15-35min, 10% -15% B; 35-60min, 15% -20% B; 60-100min, 20% -25% B; 100-120min, 25-40% B; 120 ℃ for 130min, 40-45% of B; 130-140min, 45-90% B; 140 ℃ for 150min, 90% B; the volume flow is 1.0 mL/min; the column temperature is 35 ℃; the detection wavelength is 254 nm; the sample volume is 10 mu L;
(3) the fingerprint of the semi-finished preparation of the Zhenqi Fuzheng capsule is constructed as follows: introducing the chromatogram of each batch of the semi-finished preparation of the glossy privet fruit and astragalus strengthening capsules obtained in the step (2) into 'traditional Chinese medicine chromatogram fingerprint similarity evaluation system software (2004 version)', generating a reference spectrum according to an average value by taking S1 (batch: 20191109) as a reference spectrum, setting a time window to be 0.05min, and generating the fingerprint of the semi-finished preparation of the glossy privet fruit and astragalus strengthening capsules after automatic matching;
(4) the characteristic spectrum of the semi-finished preparation of the glossy privet fruit and astragalus strengthening healthy energy capsule is constructed: and (4) selecting common chromatographic peaks with higher response values from the fingerprint obtained in the step (3) as characteristic fingerprint peaks, wherein the total number of chromatographic peaks is 12, and thus forming the characteristic spectrum of the semi-finished preparation of the Zhenqi Fuzheng capsule.
Example 2: the fingerprint construction method of the finished product preparation of the glossy privet fruit and astragalus strengthening capsules comprises the following steps:
(1) preparing a test solution: accurately weighing 1.0g of finished product preparation of the Zhenqi Fuzheng capsule, adding 20mL of pure water, carrying out ultrasonic treatment for 5min until the finished product preparation is fully dissolved, loading a macroporous resin chromatographic column with the column height of 12cm, weighing 1000mL of pure water for washing, then washing the chromatographic column with 100mL of ethanol, collecting filtrate, and carrying out rotary evaporation and concentration to 10mL to obtain a test solution of the finished product preparation of the Zhenqi Fuzheng capsule;
(2) and (3) high performance liquid chromatography detection: injecting the test solution of the finished preparation of the Zhenqi Fuzheng capsule obtained in the step (1) into a high performance liquid chromatograph, and recording a chromatogram; chromatographic conditions are as follows: an Agilent Eclipse XDB-C18 (4.6X 250mm, 5 μm) chromatography column; gradient elution with water (a) -acetonitrile (B) as mobile phase: 0-15min, 5% -10% B; 15-35min, 10% -15% B; 35-60min, 15% -20% B; 60-100min, 20% -25% B; 100-120min, 25-40% B; 120 ℃ for 130min, 40-45% of B; 130-140min, 45-90% B; 140 ℃ for 150min, 90% B; the volume flow is 1.0 mL/min; the column temperature is 35 ℃; the detection wavelength is 254 nm; the sample volume is 10 mu L;
(3) the fingerprint spectrum of the finished product preparation of the glossy privet fruit-astragalus strengthening capsules is constructed: importing the chromatograms of the finished glossy privet fruit and astragalus body resistance strengthening capsule preparations obtained in the step (2) into traditional Chinese medicine chromatogram fingerprint similarity evaluation system software (2004 version), generating a reference spectrum according to an average value by taking a certain batch of chromatograms as a reference spectrum, setting a time window to be 0.05min, and automatically matching to generate a finished glossy privet fruit and astragalus body resistance strengthening capsule preparation fingerprint;
(4) the characteristic spectrum of the finished preparation of the glossy privet fruit-astragalus strengthening capsules is constructed: and (4) selecting 28 common chromatographic peaks with higher response values from the fingerprint obtained in the step (3) as characteristic fingerprint peaks to form the finished product preparation characteristic spectrum of the Zhenqi Fuzheng capsule.
Experimental example 1: fingerprint spectrum research of semi-finished preparation of Zhenqi Fuzheng Capsule
1. Experimental materials and instruments
1.1 materials and reagents
The semi-finished preparation samples of the Zhenqi Fuzheng capsule used in the experiment are collected from Xinbang pharmaceutical Co., Ltd.
1.2 Experimental instruments
A high performance liquid chromatograph Agilent 1260; one-ten-thousandth balance XS105 DU; one in ten thousand electronic balance AL204 Mettler-Torledo instrument; an electric heating constant temperature blast drying box 101-2 AB; a micropipette Eppendorf Research plus; KQ-300DE type of numerical control ultrasonic cleaner; a pure water/ultrapure water system was prepared as clear TM-D24 UV.
2. Experimental methods
2.1 sample preparation
Accurately taking 1.0g of a semi-finished preparation of the Zhenqi Fuzheng capsule, measuring 20mL of pure water, putting the mixture into a 50mL beaker, carrying out ultrasonic treatment for 5min until the mixture is fully dissolved, loading a washed chromatographic column, measuring 1000mL of pure water for washing, then washing the chromatographic column with 100mL of ethanol, collecting filtrate, and carrying out rotary evaporation and concentration to 10mL for later use.
2.2 chromatographic conditions
An Agilent Eclipse XDB-C18 (4.6X 250mm, 5 μm) chromatography column; the mobile phase is water (A) -acetonitrile (B), gradient elution is carried out (0-15min, 5-10% B, 15-35min, 10-15% B, 35-60min, 15-20% B, 60-100min, 20-25% B, 100-120min, 25-40% B, 120-130min, 40-45% B, 130-140min, 45-90% B, 140-150min and 90% B); the volume flow is 1.0 mL/min; the column temperature is 35 ℃; the detection wavelength is 210 nm; 220 nm; 254 nm; 285 nm; the amount of the sample was 10. mu.L.
2.3 sample preparation Condition optimization
The inventor carries out optimization comparison experiments on chromatographic column parameters.
(1) Inspection of chromatographic column packing
Filling 3 different filler media of polyamide, silica gel and macroporous resin into a chromatographic column with the same specification, ensuring that the height of a filter column is consistent, balancing 0.05mol/L PBS buffer solution with pH value of 7.5 to make a base line stable, washing the chromatographic column with pure water until the pH value is not changed, adding a completely dissolved sample solution (3 parts in parallel), washing the chromatographic column with 1000mL of pure water slowly at a constant speed, measuring 200mL of ethanol, continuously washing the chromatographic column, collecting filtrate, performing rotary evaporation and concentration to 10mL, inspecting 3 different fillers of polyamide, silica gel and macroporous resin of the chromatographic column respectively, determining under optimized chromatographic conditions, and comparing the peak shape of each sample chromatographic peak and the response value of each main chromatographic peak, wherein the result is shown in figure 1.
The sample treated by the macroporous resin chromatographic column has more chromatographic peaks, higher peak shape and better effect.
(2) Sample loading amount investigation
Filling the processed macroporous resin filler medium into chromatographic columns with the same specification, paralleling 3 parts, ensuring that the washing conditions of the samples are consistent, respectively weighing 0.5g, 1.0g and 1.5g of samples, fully dissolving, then loading, weighing 1000mL of pure water, then washing with 200mL of ethanol, collecting the washing liquid, performing rotary evaporation and concentration to 10mL, performing determination under the optimized chromatographic conditions, and comparing the chromatographic peak shape and the main chromatographic peak response value of each sample, wherein the result is shown in figure 2.
When the sample loading amount is 1.0g, the sample treatment effect is good.
(3) Volume study of eluent
Filling the processed macroporous resin filler medium into chromatographic columns with the same specification, ensuring the consistent washing conditions of the samples, respectively weighing 1.0g of the samples, fully dissolving the samples, loading the samples, weighing 1000mL of pure water, washing the samples with 50mL, 100mL, 150mL, 200mL and 250mL of ethanol, collecting the washing liquid, performing rotary evaporation and concentration to 10mL, measuring under the optimized chromatographic conditions, and comparing the peak shape of each sample chromatographic peak and the response value of each main chromatographic peak, wherein the result is shown in figure 3.
When the volume of the ethanol eluent is 100mL, the sample treatment effect is better.
(4) Investigation of filler dosage
Filling the processed macroporous resin filler medium into chromatographic columns with the same specification, wherein the column filling heights are respectively 10cm, 11cm, 12cm and 13cm, ensuring that the washing conditions of the samples are consistent, respectively weighing 1.0g of the samples, fully dissolving, then loading, weighing 1000mL of pure water, washing with 200mL of ethanol, collecting the washing liquid, performing rotary evaporation and concentration to 10mL, performing determination under the optimized chromatographic conditions, and comparing the chromatographic peak shape and the main chromatographic peak response value of each sample, wherein the results are shown in FIG. 4.
When the height of the chromatographic column filler is 12cm, the sample treatment effect is better.
2.4 chromatographic wavelength investigation
4 wavelengths of 210nm, 220nm, 254nm and 285nm are selected for measurement in the experiment, and full-wavelength scanning is synchronously carried out under an ultraviolet detector. The full-wavelength scanning spectrum shows that the chromatographic peak appearing under 254nm is relatively large, most of the chromatographic peaks have relatively high absorption, and the sample chromatographic peak has relatively good peak shape and presents good resolution. Therefore, 254nm is selected as the detection wavelength of HPLC fingerprint of the semi-finished preparation of Zhenqi Fuzheng Capsule, and the result is shown in FIG 5.
3 methodology examination
Carrying out methodology verification stability, verification precision and verification repeatability experiments on the semi-finished preparation of the Zhenqi Fuzheng capsule according to relevant guiding principles of fingerprint spectrum methodology verification of Chinese pharmacopoeia.
3.1 stability Studies
Accurately weighing 1.0g of semi-finished preparation powder of the Zhenqi Fuzheng capsule, performing ultrasonic treatment for 5min to completely dissolve a sample, performing chromatography column chromatography to obtain a sample solution, performing sample injection at different intervals of 0h, 2h, 4h, 8h, 12h and 24h according to the chromatographic condition of '2.2', calculating the relative retention time of each main chromatographic peak and the RSD (%) value of the relative peak area, and performing sample stability investigation.
And carrying out a methodology verification stability experiment on the semi-finished product sample of the Zhenqi strengthening capsule according to related guiding principles of fingerprint spectrum methodology verification of Chinese pharmacopoeia. The results show that the peak areas and the retention times of the main chromatographic peaks of the semi-finished preparation of the Zhenqi Fuzheng Capsule at different time stages have no obvious change, the results are shown in FIG 6, and the RSD values of the relative retention time and the relative peak area are both less than 3.0%, which both meet the requirements of fingerprint, and show that the semi-finished preparation of the Zhenqi Fuzheng Capsule has stable chemical properties within 24 h.
3.2 precision investigation
Accurately weighing 1.0g of semi-finished preparation powder of the Zhenqi Fuzheng Capsule, performing ultrasonic treatment for 5min to completely dissolve a sample, performing chromatography column chromatography to obtain a sample solution, continuously injecting sample for 6 times under the chromatographic condition of '2.2', calculating the relative retention time of a main chromatographic peak and the RSD (%) value of a relative peak area, and performing sample precision investigation.
According to the related guiding principle of fingerprint spectrum methodology verification of Chinese pharmacopoeia, methodology verification precision experiment is carried out on the semi-finished preparation sample of the Zhenqi Fuzheng capsule, and the result is shown in figure 7. The relative retention time of each main common peak and the RSD of the relative peak area of the common peak are both less than 3.0 percent, which accords with the requirement of fingerprint spectrum and shows that the precision of experimental instruments is good.
3.3 repeatability examination
Accurately weighing 1.0g of semi-finished preparation powder of the Zhenqi Fuzheng capsule, performing ultrasonic treatment for 5min to completely dissolve a sample, performing chromatography column chromatography to obtain a sample solution, performing sample injection on the sample solution in parallel by 6 parts, performing sequence sample injection according to the chromatographic condition of '2.2', calculating the relative retention time of a main chromatographic peak and the RSD (%) value of the relative peak area, and performing sample repeatability inspection.
According to the related guiding principle of the methodology verification of the fingerprint spectrum in the Chinese pharmacopoeia, the methodology verification repeatability experiment is carried out on the semi-finished product sample of the Zhenqi strengthening capsule, and the result is shown in figure 8. The result shows that the relative retention time of each common peak and the relative peak area RSD value are both less than 3.0 percent, and the method accords with the requirement of a fingerprint spectrum, and shows that the method has good reproducibility.
4 fingerprint map establishment
The collected information of the semi-finished preparation samples of the glossy privet fruit and astragalus strengthening capsules is processed by a fingerprint evaluation software-traditional Chinese medicine chromatogram fingerprint similarity evaluation system A (2004 edition) for the semi-finished products (50 g/batch) of the glossy privet fruit and astragalus strengthening capsules in different 13 batches, and the fingerprints of the semi-finished preparation of the glossy privet fruit and astragalus strengthening capsules are established.
And (3) after the preparation of the test solution is finished according to the step 2.1, determining different batches of samples in different areas under the condition of 2.2 chromatography, and recording a chromatogram. HPLC fingerprint of 13 batches of the semi-finished preparation extract of Zhenqi Fuzheng Capsule: a traditional Chinese medicine chromatogram fingerprint similarity evaluation system A (2004 edition) is adopted, S1 (batch: 20191109) is used as a reference spectrum, a reference spectrum is generated according to an average value, a time window is set to be 0.05min, and a fingerprint is generated after automatic matching is carried out, as shown in figure 9.
5 feature map construction
Selecting high performance liquid chromatograms of samples of different batches from the fingerprints of 13 batches of the semi-finished preparation of the Zhenqi Fuzheng Capsule, selecting a common chromatographic peak with a high response value as a characteristic fingerprint peak, and finally selecting 12 peaks as characteristic peaks of the semi-finished preparation of the Zhenqi Fuzheng Capsule, wherein the characteristic chromatogram is shown in figure 10.
6 evaluation of similarity of fingerprint
And calculating the similarity between different batches of the semi-finished glossy privet fruit and astragalus strengthening capsules by using the similarity evaluation software of the fingerprint of the traditional Chinese medicinal materials of the State pharmacopoeia Commission. The similarity of the chromatogram of the semi-finished preparation extract of glossy privet fruit and astragalus strengthening capsules of 13 batches and the similarity of the comparison fingerprint chromatogram of the collected samples of 13 batches are all larger than 0.9, as shown in the table 1-1, and the requirement of the fingerprint is met.
TABLE 1-1 similarity of semi-finished preparation of Zhenqi Fuzheng Capsule
7 statistical analysis
Fingerprint similarity evaluation software-traditional Chinese medicine chromatogram fingerprint similarity system A (2004 edition) is adopted to establish a fingerprint of the semi-finished preparation of the Zhenqi Fuzheng capsule, and clustering analysis is carried out by taking the common peak-to-peak area of each batch of the semi-finished preparation of the Zhenqi Fuzheng capsule as a clustering parameter by virtue of IBM SPSS Statistics 21 software. The analysis is carried out by taking 12 common peaks of 13 batches of the semi-finished preparation of the Zhenqi Fuzheng capsule as clustering variables, wherein the common peaks 2, 4 and 7 have larger peak areas, and the peak areas of the common peaks are larger than 90% of the peak area of the common peaks, as shown in figure 11, S01, S02, S03, S04, S06, S07, S08, S09, S10, S11 and S13 are class I. The semi-finished preparation of Zhenqi Fuzheng capsule in S05 and S12 batches is type II. The common peak area of the II type is relatively high, and the common peak areas of the two types are similar, which indicates that the contents of common peak compounds of HPLC (high performance liquid chromatography) spectra of 13 batches of the glossy privet fruit and astragalus strengthening capsule semi-finished product preparation are basically consistent.
8 small knot
Establishing a characteristic spectrogram of the semi-finished preparation of the Zhenqi Fuzheng capsule according to the liquid chromatogram, the main chromatographic peak and the characteristics of the relative retention time of the semi-finished preparation of the Zhenqi Fuzheng capsule of different batches, determining the common peak of the semi-finished preparation of the Zhenqi Fuzheng capsule of different batches, and carrying out cluster analysis by taking the peak area of the common chromatographic peak as a clustering parameter. The results show that certain differences exist among different batches of the semi-finished preparation of the Zhenqi Fuzheng capsule, which provides a certain theoretical basis for judging the quality of different batches of samples, and the correlation between each common chromatographic peak and the biological activity of the preparation needs to be further verified.
Experimental example 2: fingerprint spectrum research of finished product preparation of Zhenqi Fuzheng Capsule
1. Experimental materials and instruments
1.1 materials and reagents
13 finished product preparation samples of the Zhenqi Fuzheng capsules of batches are collected from Xinbang pharmaceutical Co., Ltd, Guizhou.
1.2 Experimental instruments
A high performance liquid chromatograph Agilent 1260; one-ten-thousandth balance XS105 DU; one in ten thousand electronic balance AL204 Mettler-Torledo instrument; an electric heating constant temperature blast drying box 101-2 AB; a micropipette Eppendorf Research plus; KQ-300DE type of numerical control ultrasonic cleaner; a pure water/ultrapure water system was prepared as clear TM-D24 UV.
2 method of experiment
2.1 sample preparation
Accurately taking 1.0g of finished preparation of the Zhenqi Fuzheng capsule, measuring 20mL of pure water, putting the mixture into a 50mL beaker, carrying out ultrasonic treatment for 5min until the mixture is fully dissolved, loading the washed chromatographic column, measuring 1000mL of pure water for washing, then washing the chromatographic column with 100mL of ethanol, collecting filtrate, and carrying out rotary evaporation and concentration to 10mL for later use.
2.2 chromatographic conditions
Agilent Eclipse XDB-C18 (4.6X 250mm, 5 μm) chromatography column. The mobile phase is water (A) -acetonitrile (B), gradient elution is carried out (0-15min, 5-10% B, 15-35min, 10-15% B, 35-60min, 15-20% B, 60-100min, 20-25% B, 100-120min, 25-40% B, 120-130min, 40-45% B, 130-140min, 45-90% B, 140-150min and 90% B); the volume flow is 1.0 mL/min; the column temperature is 35 ℃; the detection wavelength is 254 nm; 210 nm; 285 nm; the amount of the sample was 10. mu.L.
3 methodology examination
Carrying out methodology verification stability, verification precision and verification repeatability experiments on finished glossy privet and astragalus strengthening capsule preparations according to relevant guiding principles verified by fingerprint spectrum methodology in Chinese pharmacopoeia.
3.1 stability Studies
Accurately weighing 1.0g of finished preparation powder of the Zhenqi Fuzheng capsule, preparing a sample according to the condition of 2.1 to obtain a test solution, injecting samples for 0h, 2h, 4h, 8h, 12h and 24h under the condition of 2.2 chromatography, calculating the relative retention time of a main chromatographic peak and the RSD (%) value of the relative peak area, and carrying out methodology verification stability investigation.
The result shows that the statistical peak area and the retention time of each main common peak have no obvious change, the relative retention time RSD value and the relative peak area RSD value of each common peak are both less than 3 percent and both meet the requirement of a fingerprint, and as shown in figure 12, the chemical property of the finished product preparation solution of the Zhenqi Fuzheng capsule is stable within 24 hours.
3.2 precision investigation
Accurately weighing 1.0g of finished preparation powder of the Zhenqi Fuzheng capsule, preparing a sample according to the condition of 2.1 to obtain a test sample solution, continuously feeding samples for 6 times under the condition of 2.2 chromatography, detecting to obtain a liquid chromatogram, calculating the relative retention time and the relative peak area RSD (%) of a main chromatographic peak, and carrying out methodology verification precision investigation.
The result is shown in figure 13, the relative retention time RSD value and the relative peak area RSD value of each common peak are both less than 3 percent, which accords with the requirement of fingerprint spectrum and shows that the precision of the instrument is good.
3.3 repeatability examination
Accurately weighing 1.0g of finished preparation powder of the Zhenqi Fuzheng capsule, preparing a sample according to the condition of 2.1 to obtain a test solution, parallelly weighing 6 parts of the test solution, continuously feeding samples under the condition of 2.2 chromatography, detecting to obtain a liquid chromatogram, calculating the relative retention time of a main chromatographic peak and the RSD (%) value of the relative peak area, and carrying out methodology verification repeatability investigation.
The result is shown in fig. 14, the relative retention time RSD value and the relative peak area RSD value of each common peak are both less than 3%, and both accord with the requirement of the fingerprint, which indicates that the method has good reproducibility.
4 fingerprint map establishment
Collecting 13 batches of finished glossy privet fruit and astragalus membranaceus body resistance strengthening capsules (50 g/batch), carrying out vacuum freeze drying on samples, accurately weighing 1.0g of finished glossy privet fruit and astragalus membranaceus body resistance strengthening capsule preparation powder, preparing sample solution according to the extraction method under the item '2.1', sampling and collecting sample chromatographic information of each batch of finished glossy privet fruit and astragalus membranaceus body resistance strengthening capsule preparations under the chromatographic condition under the item '2.2', and obtaining the fingerprint of the finished glossy privet fruit and astragalus membranaceus body resistance strengthening capsule preparation by means of fingerprint similarity evaluation software. The finished product preparation extracts of the glossy privet fruit and astragalus strengthening capsules of 13 batches are measured, chromatograms are recorded, and fingerprints of finished product preparation samples of the glossy privet fruit and astragalus strengthening capsules are established by using software of a traditional Chinese medicine chromatogram fingerprint similarity evaluation system A (2004 edition), as shown in figure 15.
5 feature map construction
The fingerprint spectra of 13 batches of the finished preparation extracts of the Zhenqi Fuzheng Capsule are marked with the aid of fingerprint spectrum similarity evaluation software, and 28 chromatographic peaks are used as characteristic peaks of the finished preparation of the Zhenqi Fuzheng Capsule, wherein the characteristic spectra are shown in figure 16.
6 evaluation of similarity of fingerprint
Preparing 13 batches of finished product preparation extracts of the glossy privet fruit and astragalus strengthening capsules under the condition of 2.1 to obtain a test solution, and measuring under the condition of 2.2 chromatography. Establishing a common mode fingerprint matching graph by using the similarity evaluation software of the fingerprints of the traditional Chinese medicinal materials in the State pharmacopoeia Commission. And obtaining a calculation result of HPLC fingerprint similarity of the Zhenqi Fuzheng capsule through software calculation. The similarity of the chromatogram of the finished preparation extract of the Zhenqi Fuzheng Capsule of 13 batches and the similarity of the comparison fingerprint chromatogram of the collected 13 batches is more than 0.9, as shown in the table 2-1, and the similarity meets the requirement of the fingerprint.
TABLE 2-113 batches of Zhenqi Fuzheng Capsule for improving body resistance
7 statistical analysis
Fingerprint similarity evaluation software-traditional Chinese medicine chromatogram fingerprint similarity evaluation system A (2004 edition) is adopted to establish the finished product preparation fingerprint of the Zhenqi Fuzheng capsule and establish the characteristic spectrum thereof, and the IBM SPSS statics 21 software is used for cluster analysis by taking the common peak area of the fingerprint as reference. And (3) analyzing by taking 28 common peaks of 13 batches of finished preparation of the Zhenqi Fuzheng capsules as clustering variables, wherein the common peaks 1, 12, 15, 19 and 20 have larger peak areas, and the peak areas of the common peaks are more than 90% of the peak area of the common peaks. As shown in fig. 17, the cluster analysis results show that S01, S02, S03, S10, and S11 are class i. The finished preparation of Zhenqifuzheng capsules of S04, S05, S06, S07, S08, S09 and S12 is class II. The common peak areas of the two types are similar, which indicates that the contents of the compounds of the common peak of the HPLC spectra of the finished product preparation of the Zhenqi Fuzheng capsule of 13 batches are basically consistent.
8 small knot
Establishing a characteristic spectrogram of the finished product preparation of the Zhenqi Fuzheng Capsule according to the liquid chromatogram, the main chromatographic peaks and the characteristics of the relative retention time of the 13 finished product preparations of the Zhenqi Fuzheng Capsule, determining the common peaks of the samples of different batches, and performing cluster analysis on the common chromatographic peak areas of the samples of different batches by taking the common chromatographic peak areas as clustering parameters. The clustering analysis result of the finished preparation of the Zhenqi Fuzheng capsule of different batches shows that the clustering result has certain correlation with the content of each main active ingredient measured by a spectrophotometry, which provides a certain theoretical basis for judging the quality of samples of different batches, and the correlation between the single peak area of the common peak and the bioactivity needs to be further analyzed and verified.
Experimental example 3: spectrogram analysis of Zhenqi Fuzheng Capsule at different production stages
The spectrogram of the Zhenqi Fuzheng Capsule at different production stages is shown in fig. 18, the light-colored marks indicate that the two medicinal materials contain chemical components together, and by comparison, the loss of the compound in the preparation process can be seen at the circle marks, while no obvious change exists between the semi-finished product and the finished product of the Zhenqi Fuzheng Capsule.
Therefore, the quality control of the semi-finished preparation and the finished preparation can be considered to be integrated into a system, and the quality control of the medicament at the early stage of production can be enhanced.
Claims (10)
1. The fingerprint construction method of the semi-finished product and the finished product preparation of the Zhenqi Fuzheng capsule is characterized by comprising the following steps:
(1) preparing a test solution: weighing the semi-finished preparation or finished preparation of the Zhenqi Fuzheng capsule, adding pure water, dissolving with ultrasound, loading on a chromatographic column, washing with pure water, washing the chromatographic column with ethanol, collecting filtrate, and concentrating by rotary evaporation to obtain a test solution;
(2) and (3) high performance liquid chromatography detection: injecting the test solution obtained in the step (1) into a high performance liquid chromatograph, and recording a chromatogram;
(3) constructing a fingerprint spectrum: processing the chromatographic information of the semi-finished preparation or finished preparation of the glossy privet fruit and astragalus strengthening capsules of a plurality of batches by adopting fingerprint similarity evaluation software to obtain fingerprints of the semi-finished preparation or finished preparation of the glossy privet fruit and astragalus strengthening capsules;
(4) constructing a characteristic spectrum: and (4) selecting a common chromatographic peak with a high response value from the fingerprint obtained in the step (3) as a characteristic fingerprint peak to form a characteristic spectrum.
2. The fingerprint construction method of the semifinished product and the finished product preparation of the Zhenqi Fuzheng capsule of claim 1, wherein the fingerprint construction method comprises the following steps: the step (1) of preparing the test solution specifically comprises the following steps: accurately taking 0.5-1.5g of the semi-finished preparation or finished preparation of the Zhenqi Fuzheng capsule, adding 20mL of pure water, performing ultrasonic treatment for 5min until the mixture is completely dissolved, loading the washed chromatographic column, washing the chromatographic column with 1000mL of pure water, washing the chromatographic column with 50-200mL of ethanol, collecting the filtrate, and performing rotary evaporation and concentration to 10mL to obtain a test solution.
3. The fingerprint construction method of the semi-finished product and the finished product preparation of Zhenqi Fuzheng capsule of claim 2, which is characterized in that: the sample amount of the semi-finished preparation or the finished preparation of the glossy privet fruit and astragalus strengthening capsules is 1.0 g; the chromatographic column filler is macroporous resin; the height of the chromatographic column packing is 12 cm; the volume of ethanol eluent was 100 mL.
4. The fingerprint construction method of the semi-finished product and finished product preparation of Zhenqi Fuzheng capsule according to claim 1 or 2, which is characterized in that: in the step (2), the high performance liquid chromatography conditions are as follows: using a C18 chromatographic column; the mobile phase A is water, the mobile phase B is acetonitrile, and gradient elution is carried out; the volume flow is 1.0 mL/min; the column temperature is 35 ℃; the detection wavelength is 210nm-285 nm; the amount of the sample was 10. mu.L.
5. The fingerprint construction method of the semifinished product and the finished product preparation of Zhenqi Fuzheng capsule of claim 4, wherein the fingerprint construction method comprises the following steps: in the step (2), the gradient elution process is as follows: 0-15min, 5% -10% B; 15-35min, 10% -15% B; 35-60min, 15% -20% B; 60-100min, 20% -25% B; 100-120min, 25-40% B; 120 ℃ for 130min, 40-45% of B; 130-140min, 45-90% B; 140 ℃ for 150min, 90% B; the detection wavelength is 254 nm.
6. The fingerprint construction method of the semi-finished product and finished product preparation of Zhenqi Fuzheng capsule according to claim 1 or 2, which is characterized in that: in the step (3), software 2004A version of the traditional Chinese medicine chromatogram fingerprint similarity evaluation system is adopted, a certain batch of chromatograms are used as reference atlases, a reference atlas is generated according to an average value, a time window is set to be 0.05min, and the fingerprints are generated after automatic matching.
7. The fingerprint of the semi-finished preparation of the Zhenqi Fuzheng capsule obtained by the method of any one of claims 1 to 6.
8. The fingerprint spectrum of the finished product preparation of the Zhenqifuzheng capsule obtained by the method of any one of claims 1 to 6.
9. The semi-finished preparation of Zhenqi Fuzheng Capsule obtained by the method of any one of claims 1-6 has a characteristic map, and the map has 12 characteristic fingerprint peaks.
10. The finished product preparation of the Zhenqifuzheng capsule obtained by the method of any one of claims 1 to 6 has a characteristic spectrum, and the spectrum has 28 characteristic fingerprint peaks.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010820383.2A CN112147242A (en) | 2020-08-14 | 2020-08-14 | Fingerprint spectrum construction method for semi-finished product and finished product preparation of Zhenqi Fuzheng capsule |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010820383.2A CN112147242A (en) | 2020-08-14 | 2020-08-14 | Fingerprint spectrum construction method for semi-finished product and finished product preparation of Zhenqi Fuzheng capsule |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112147242A true CN112147242A (en) | 2020-12-29 |
Family
ID=73887514
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010820383.2A Pending CN112147242A (en) | 2020-08-14 | 2020-08-14 | Fingerprint spectrum construction method for semi-finished product and finished product preparation of Zhenqi Fuzheng capsule |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112147242A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102621244A (en) * | 2012-03-27 | 2012-08-01 | 中山大学 | Construction method for HPLC (high performance liquid chromatography) finger-print chromatogram of ginseng and astragalus strengthening injection and application of finger-print |
| WO2014067478A1 (en) * | 2012-11-02 | 2014-05-08 | 丽珠集团利民制药厂 | Method for establishing shenqi fuzheng injection fingerprint spectrum |
| CN108982738A (en) * | 2018-10-24 | 2018-12-11 | 吉林省现代中药工程研究中心有限公司 | The intermediate characteristic spectrum of zhenqi fuzheng granules or the construction method and detection method of zhenqi fuzheng granules finger-print |
| US20190219550A1 (en) * | 2012-11-02 | 2019-07-18 | Li Min Pharmaceutical Factory Of Livzon Pharmaceutical Group | System and Method for Identifying Shenqi Fuzheng Injection |
-
2020
- 2020-08-14 CN CN202010820383.2A patent/CN112147242A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102621244A (en) * | 2012-03-27 | 2012-08-01 | 中山大学 | Construction method for HPLC (high performance liquid chromatography) finger-print chromatogram of ginseng and astragalus strengthening injection and application of finger-print |
| WO2014067478A1 (en) * | 2012-11-02 | 2014-05-08 | 丽珠集团利民制药厂 | Method for establishing shenqi fuzheng injection fingerprint spectrum |
| US20190219550A1 (en) * | 2012-11-02 | 2019-07-18 | Li Min Pharmaceutical Factory Of Livzon Pharmaceutical Group | System and Method for Identifying Shenqi Fuzheng Injection |
| CN108982738A (en) * | 2018-10-24 | 2018-12-11 | 吉林省现代中药工程研究中心有限公司 | The intermediate characteristic spectrum of zhenqi fuzheng granules or the construction method and detection method of zhenqi fuzheng granules finger-print |
Non-Patent Citations (3)
| Title |
|---|
| 崔方等: "高效液相色谱法测定贞芪扶正制剂中8种生物活性化合物的含量", 《药物分析杂志》 * |
| 张勇慧等: "参芪扶正注射液中党参药材HPLC指纹图谱研究", 《中药材》 * |
| 魏晓雨等: "贞芪扶正颗粒的HPLC指纹图谱研究", 《中国药房》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108717095B (en) | Detection method, identification method and content determination method of loquat leaves or medicine containing loquat leaf raw material | |
| CN111337589A (en) | Method for establishing orange-shell mixture HPLC fingerprint spectrum | |
| CN107478749B (en) | Detection method of medicinal preparation of Taohong Siwu decoction | |
| CN107607649B (en) | Method for detecting periploca forrestii schltr | |
| CN103336078B (en) | Method for measuring content of cough-relieving and anti-asthma pill | |
| CN101766771A (en) | Quality control method of medicine for gynecopathy treatment | |
| CN104034821B (en) | The detection of bitter buckwheat or its product and discrimination method | |
| CN107589191A (en) | Golden shellfish oral liquid HPLC finger-prints are established and detection method | |
| CN100437112C (en) | Method for inspecting Chinese medicinal preparation quality in treatment of old man eyes dieases | |
| CN103472148B (en) | Fingerprint spectrum detection method of Chinese medicine composition preparation | |
| CN102441057B (en) | High performance liquid chromatography (HPLC) fingerprint detection method for blood-nourishing brain-refreshing grain | |
| CN105510488B (en) | A kind of finger-print and its quality determining method for relieving pain patch | |
| CN104316613A (en) | Method for establishing fingerprint spectrum of wind-dispelling and detoxifying capsules | |
| CN107688072A (en) | A kind of detection method of XINGNAOJING ZHUSHEYE | |
| CN112147242A (en) | Fingerprint spectrum construction method for semi-finished product and finished product preparation of Zhenqi Fuzheng capsule | |
| CN105588888A (en) | Traditional Chinese medicine fingerprint spectrum quality control and measuring method | |
| CN113759057B (en) | Characteristic spectrum of allium macrostemon white water extract and preparation thereof and construction method thereof | |
| CN110274970A (en) | The method for building up for melting poor finger-print and its application in Yixiesheng capsule Quality Control | |
| CN106918673A (en) | A kind of method for building up of the finger-print of Chinese medicine composition | |
| CN113866309A (en) | HPLC fingerprint spectrum establishment method and application of 11 bufogenin components in venenum bufonis | |
| CN109358120B (en) | Method for detecting characteristic spectrum of sophora fruit preparation | |
| CN107976494B (en) | Construction of standard characteristic spectrum of Kangfu tincture and quality detection method thereof | |
| CN112924600B (en) | Fingerprint spectrum establishment method of preparation for treating joint swelling and pain caused by rheumatic arthralgia | |
| CN104133028A (en) | Establishment method for high performance liquid chromatography fingerprint of Rubia cordifolia root formula granule and standard fingerprint thereof | |
| CN111474276B (en) | Quality control method of yang invigorating tablet preparation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20201229 |
|
| RJ01 | Rejection of invention patent application after publication |