CN112147198A - Functional porous membrane material and application thereof in complex carbohydrate chain molecule recognition - Google Patents
Functional porous membrane material and application thereof in complex carbohydrate chain molecule recognition Download PDFInfo
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
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- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J7/00—Chemical treatment or coating of shaped articles made of macromolecular substances
- C08J7/12—Chemical modification
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
- G01N27/3278—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction involving nanosized elements, e.g. nanogaps or nanoparticles
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
- C08J2367/02—Polyesters derived from dicarboxylic acids and dihydroxy compounds
Abstract
The invention provides a functional porous membrane material, a preparation method and application thereof in recognition and detection of monosaccharide and sugar chain molecules. Firstly, a functional polymer is synthesized in a solution, and then the functional polymer is grafted into the pore channel of the inorganic porous membrane or the organic porous membrane by using a coupling reaction. The polymer membrane in the pore canal has specific interaction with monosaccharide and sugar chain molecules, and can cause the polymer layer to shrink in different degrees, the change of transmembrane ion current before and after the sugar chain molecule treatment can be detected by using a picometer, and the detection of different monosaccharide and sugar chain molecules can be realized through the amplitude of the current change. The material is used for detecting monosaccharide or sugar chain, has the advantages of simple operation, high sensitivity, high detection speed and no need of marking, and provides a favorable tool for sugar chain structure analysis in glycobiology.
Description
Technical Field
The invention relates to modification of a polymer on the surface of inorganic and organic porous membrane materials, and also relates to the application field of the polymer-modified porous membrane material in detection and analysis of different monosaccharides and sugar chains, in particular to a functional porous membrane material and application thereof in identification of complex sugar chain molecules.
Background
Carbohydrates serve as an important class of biomolecules beyond proteins and nucleic acids, and their structural support and energy supply roles have been fully revealed. However, saccharides, especially various oligosaccharide chains, which are important information substances in the body, participate in almost all cell biological activities, and particularly play specific recognition, mediation and regulation roles in life and disease processes such as cell differentiation, development, immunity, aging, canceration, information transmission, and the like. Oligosaccharide chains of organisms exist on the cell surface mainly in the form of glycoproteins, glycolipids and glycosaminoglycans, constituting a coating of carbohydrates. In glycoproteins, sugar chains represent a small portion of the entire glycoprotein, but have important physiological functions that affect all conformationally-determined functions, such as proper protein folding, intracellular localization, antigenicity, cell adhesion, and pathogen binding, by affecting the overall conformation of the protein. Of the mammalian proteins, about 50% of the proteins are glycosylated to varying degrees. From the medical point of view, the degree of glycosylation of proteins and the modification of sugar chain structure are closely related to various diseases. For example, sialic acid sugar chains are overexpressed on the surface of cancer cells, and the sugar chains overexpressed in different cancers usually have different structures, so that specific biomarkers are provided for early diagnosis, prognosis and treatment targets of cancers, and the biomarkers, such as Her2/Neu in breast cancer and AFP in liver cancer, are glycoproteins.
Currently, most of glycoconjugates are known to be composed of 2-20 monosaccharides connected in different types and numbers, different glycosidic bonds, different arrangement orders, even different branching forms, and the like. It follows that the number of sugar chain isomers formed by different monosaccharides far exceeds the number of isomers formed by proteins or nucleotides, and that as the number of monosaccharides increases, the number of sugar chain isomers will increase geometrically. Therefore, this structural diversity allows sugar chains to carry information far beyond proteins and nucleic acids, and also allows sugar biology and glycomics to develop great challenges in revealing sugar chain structures and further associating sugar chain functions. At present, the sugar chain structure analysis method mainly comprises special glycosidase enzymolysis and sugar chain derivatization ways, and electrophoresis, chromatography, mass spectrum, nuclear magnetism or combination of several means are utilized simultaneously. These approaches then have significant limitations, such as the need for expensive equipment, complex and time-consuming operations, high experience requirements for the analyst, and high sample requirements. Therefore, it is very urgent to develop a sugar chain recognition and detection system or analysis method which is simple, easy, highly sensitive and rapid.
In recent years, the research of the bionic ion nano-channel is flourishing day by day, and the artificial nano-channel can simulate the on-off of the biological ion channel, thereby providing an application strategy of molecular recognition sensing. Therefore, in the present invention, we innovatively use nanochannels for recognition and discrimination of monosaccharide molecules as well as sugar chain molecules, and develop a sugar chain detection device with high sensitivity. By grafting the functional polymer with the specific recognition function on the sugar molecules in the nanometer pore canal, different sugar molecules can induce the grafted polymer to shrink to different degrees, so that the ion current flowing through the nanometer pore canal is obviously changed, and finally, the detection of different monosaccharides and even different chain structure sugar chain molecules is realized. The present invention provides a means for sugar chain detection analysis and provides a strategy for development of sugar chain detection devices.
Disclosure of Invention
The invention aims to provide a polymer with a specific recognition function on sugar molecules, and the polymer is grafted into the pore canals of inorganic and organic nano porous membranes so as to prepare a polymer nano channel membrane, wherein the polymer nano channel membrane can be used for sugar molecule recognition detection and accurate distinguishing of different sugar molecules. Compared with the traditional electrochemical and mass spectrometric detection method, the polymer modified porous membrane material has the advantages of simple operation, high sensitivity, high detection speed and no need of marking when detecting monosaccharide or sugar chain molecules. Is very suitable for sugar biological sugar chain structure detection and analysis.
The technical scheme adopted by the invention is as follows:
a functional porous membrane material comprising a porous membrane and a functional polymer grafted to the interior surface of the pore channels of the porous membrane, the polymer having the following molecular structure:
wherein, the main chain polymer polyethyleneimine (branched type, molecular weight 10000-60000), the grafting amount is 1-99% (taking the number of primary amino groups as a base number), and n is 20-120;
the porous membrane is an inorganic porous membrane or an organic porous membrane;
the diameter of the porous membrane material is 5-50 mm, and the average pore diameter is 20-200 nm;
the inorganic porous membrane is anodic aluminum oxide (PAA) or silicon nitride (Si)3N4) One of (1);
the organic porous membrane is one of polyethylene terephthalate (PET), Polyimide (PI) and Polycarbonate (PC).
A preparation method of a functional porous membrane material, the preparation method grafts functional recognition unit-glucoside molecule on polymer skeleton, then grafts the polymer with recognition unit to the inner surface of the pore channel of the porous membrane, the concrete steps are as follows:
(1) dissolving 1g of polyethyleneimine (branched type, molecular weight of 10000-60000) in 20ml of ethanol to prepare a polymer solution; weighing 0.5-1.5 g of 4-formylphenyl-beta-D-glucoside, adding into the polymer solution, and reacting at room temperature for 24 hours; then putting the reaction solution into a dialysis bag (molecular weight cut-off is 3500), dialyzing in water for 5 days, and freeze-drying to obtain the required polymer;
(2) modifying the inorganic porous membrane: placing the inorganic porous membrane modified with isothiocyanate groups into a flask, adding 10mL of methanol/water solution (v/v is 1/1) dissolved with 0.2g of polymer, and reacting at 60 ℃ for 24 h; taking out, washing with a large amount of deionized water, and blow-drying with nitrogen to obtain a polymer modified inorganic porous membrane for later use;
(3) modifying the organic porous membrane: placing the organic porous membrane into a flask, dissolving 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) in MES (2-N-morpholine ethanesulfonic acid monohydrate) buffer (pH 5.5, 0.1mol/L), and activating the organic porous membrane for 1 h; and then thoroughly cleaning the organic porous membrane by using MES buffer solution, immersing the organic porous membrane into 2ml of MES buffer solution dissolved with 40mg of polymer, reacting for 24 hours at room temperature, taking out the organic porous membrane, cleaning the organic porous membrane by using a large amount of deionized water, and drying the organic porous membrane by using nitrogen to obtain the polymer modified organic porous membrane for later use.
The application of a functional porous membrane material in the identification of complex sugar chain molecules, and the application of the porous membrane material in the high-sensitivity detection of monosaccharides and sugar chains.
The specific steps of the porous membrane material in the high-sensitivity detection application of monosaccharide and sugar chain are as follows:
and 2, removing the electrolyte added in the step 1, adding the electrolyte containing sugar molecules with different types and different concentrations again, standing for 5-15 minutes, inserting electrodes at two ends of the electrochemical cell, and measuring transmembrane current of the electrochemical cell by using a Peak meter.
In the step 1 and the step 2, the electrolyte is 0.01-2.0 mol/L sodium chloride or potassium chloride solution, the pH of the electrolyte is 2-10, and the solvent is deionized water solution.
The electrode in the step 1 is a silver-silver chloride electrode, a mercury-mercury chloride electrode, graphite or platinum wire electrode; in the step 2, the electrode is a silver-silver chloride electrode, a mercury-mercury chloride electrode or a graphite electrode.
When the change of transmembrane ion current is measured by adopting a picometer in the step 1 and the step 2, a power supply applies scanning voltage of-0.2 to +0.2V to two ends of an electrode, and the duration time of each voltage is 1-2 s.
The invention has the technical advantages that:
1. the polymer used by the polymer modified porous membrane material prepared by the invention is prepared by utilizing a commercial polymer main chain coupling recognition unit before grafting, and has the advantages of simple preparation, low cost and strong expandability;
2. when the polymer modified porous membrane material prepared by the invention is used for identifying and detecting sugar molecules, the responsiveness to different monosaccharide molecules and different connecting sugar chain molecules is different, and the accurate identification and differentiation of different monosaccharides and sugar chains can be realized;
3. when the polymer modified porous membrane material prepared by the invention is used for detecting sugar molecules, signals are micro-currents, the integration and the expansibility are good, and a foundation can be provided for development of detection devices.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 is a schematic diagram of a polymer modified porous membrane;
FIG. 2 is an AFM image of the surface of a porous membrane before and after polymer modification;
FIG. 3 thermogravimetric analysis of porous membranes before and after polymer modification;
FIG. 4 XPS plots of porous membranes before and after polymer modification;
FIG. 5 is a graph showing the voltage-current curve before and after the polymer-modified inorganic porous membrane;
FIG. 6 is a graph showing the change of transmembrane current with concentration after the polymer modified inorganic porous membrane is treated by adding different monosaccharides;
FIG. 7 is a graph of transmembrane current as a function of concentration for polymer-modified inorganic porous membranes treated with different disaccharide chains;
FIG. 8 is a graph of transmembrane current increasing with concentration after treatment of a polymer modified inorganic porous membrane with different trisaccharide chains;
FIG. 9 is a graph showing the voltage-current curve before and after the polymer modification of the organic porous film;
FIG. 10 is a graph showing the voltage-current curves of polymer modified organic porous membranes with different sugar molecules.
Detailed Description
In order to make the contents, technical solutions and advantages of the present invention more apparent, the present invention is further described below with reference to specific embodiments and drawings, and these embodiments are merely used to illustrate the present invention, and the present invention is not limited to the following embodiments.
Synthesis of Polymer:
example 1
The polymer structure is shown in figure 1, 1g of polyethyleneimine (branched type, molecular weight is 10000g/moL) is dissolved in 20ml of ethanol, 0.5g of 4-formylphenyl-beta-D-glucoside is weighed and added into the aqueous solution, and the mixture is stirred at room temperature and reacts for 24 hours; then filling the reaction solution into a dialysis bag (the molecular weight cutoff is 3500g/moL), putting the dialysis bag into water for dialysis for 5 days, and freeze-drying to obtain the polymer (PEI-g-Gls for short).
Preparing a polymer modified porous membrane, namely preparing a functional porous membrane:
example 2
(1) A schematic representation of a polymer modified porous membrane is shown in figure 1. Taking a porous alumina membrane (PAA) as an example, firstly, the PAA membrane is cleaned thoroughly by using ethanol ultrasound, dried by blowing nitrogen, cleaned for 10 minutes by using an ultraviolet ozone cleaner, placed in an Erlenmeyer flask, 0.5mL of 3- (triethoxysilane) propyl isothiocyanate (Cas No.:58698-89-8) is dissolved in 10mL of toluene, added into the flask, and passes through the PAA membrane, heated to reflux and reacted for 6 hours. After the reaction is finished, the reaction kettle is taken out, is thoroughly washed by toluene and dichloromethane in sequence, is dried by nitrogen, and is placed in a clean flask again. 10mL of PEI-g-Gls solution (20mg/mL, methanol/water, v/v. 1/1) was prepared, added to the flask, and reacted at 60 ℃ for 24h without passing through the PAA membrane. After the reaction is finished, taking out the PAA membrane, thoroughly cleaning with a large amount of deionized water, and blow-drying to obtain a polymer modified inorganic porous membrane material;
(2) the surface topography of the polymer modified inorganic porous membrane is shown in figure 2, and the pore diameter change of the porous membrane can be obviously seen before and after the polymer modification;
(3) thermogravimetric analysis of the polymer modified inorganic porous membrane is shown in fig. 3, and it can be obviously seen that the polymer modified porous membrane generates apparent weight loss;
(4) the analysis of the polymer modified inorganic porous membrane by X-ray photoelectron spectroscopy (XPS) is shown in FIG. 4, and the N1s and C1s peaks after the polymer modification can be clearly seen;
example 3:
the organic porous film is exemplified by a polyethylene terephthalate porous film (PET) after latent track etching. Firstly, thoroughly cleaning a PET film with ethanol, drying the PET film with nitrogen, and placing the PET film into a triangular flask; 70mg of 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride (EDC) and 45mg of N-hydroxysuccinimide (NHS) were weighed out and dissolved in 10mL of MES (2-N-morpholinoethanesulfonic acid monohydrate) buffer (pH 5.5, 0.1mol/L), and added to the flask to activate the PET film for 1 h; the PET membrane was then thoroughly washed with MES buffer and blown dry with nitrogen. Then, the mixture was immersed in 10mL of a polymer solution (20mg/mL, MES buffer) and reacted at room temperature for 24 hours. And (3) taking out the PET membrane after the reaction is finished, washing the PET membrane by using a large amount of deionized water, and drying the PET membrane by using nitrogen to obtain the polymer modified organic porous membrane material.
The application of the polymer modified porous membrane in sugar molecule detection:
example 4
(1) A polymer modified inorganic porous membrane material takes a PEI-g-Gls modified PAA membrane as an example, the membrane is held between two self-made electrochemical tanks, 0.01M sodium chloride solution is filled on two sides of the membrane, an electrode (a silver/silver chloride electrode is taken as an example) is inserted, ion current passing through the membrane is measured by a picometer, and the voltage range is-0.2V. The transmembrane current change before and after the polymer modification is shown in fig. 5, and it can be seen that the transmembrane ionic current of the PAA membrane becomes smaller due to the smaller pore diameter of the nanochannel after the polymer modification.
(2) A0.01M sodium chloride solution was used as a solvent to prepare a sialic acid solution (Neu5Ac, concentration 1X 10)-3M). 0.01M sodium chloride solution with dissolved sialic acid was added to both sides of the electrochemical cell, and after waiting for 10min, electrodes were inserted and transmembrane ionic current was measured. The current profile is shown in fig. 5, and it is evident that the pore size of the nanochannel increased after sialic acid was added, resulting in an increase in current. Therefore, the recognition and detection of sialic acid can be realized by utilizing the characteristic of the current increase.
Example 5
Taking 0.01M sodium chloride solution as a solvent, and preparing sialic acid, galactose, glucose and mannose solutions with different concentrations. For a sugar molecule, a porous membrane modified by a polymer is held in the middle of a self-made electrochemical cell, sugar solutions with the concentration from small to large are sequentially added to two sides of the electrochemical cell, the cell waits for 10min, then electrodes are inserted, and transmembrane ionic current is measured once after each addition of the solution. The current increase proportion and the concentration increase condition are plotted, as shown in fig. 6, it can be clearly seen that the ion current increase conditions of the nanochannel caused by different sugar molecules are obviously different, and by utilizing the different current change characteristics, the polymer modified nanochannel realizes the identification and detection of different monosaccharide molecules.
Example 6
Solutions of 2, 3-disaccharide chains (Neu5 Ac. alpha.2, 3 Gal. beta.MP,) and 2, 6-disaccharide chains (Neu5 Ac. alpha.2, 6 Gal. beta.MP) were prepared at different concentrations using 0.01M sodium chloride solution as a solvent. Aiming at a sugar chain molecule, a porous membrane modified by a polymer is arranged in the middle of a self-made electrochemical tank, sugar chain solutions with the concentration from small to large are sequentially added to two sides of the electrochemical tank, the waiting time is 10min, then electrodes are inserted, and transmembrane ionic current is measured once after each solution addition. The current increase ratio and the concentration increase condition are plotted, as shown in fig. 7, it can be clearly seen that the ion current increase conditions of the nanochannel caused by different sugar chain molecules are obviously different, and by utilizing the different current change characteristics, the polymer modified nanochannel realizes the identification and detection of different sugar chain molecules.
Example 7
Different concentrations of 2, 3-oligosaccharide (Neu5Ac α 2,3Gal β 1,4Glc) and 2, 6-oligosaccharide (Neu5Ac α 2,6Gal β 1,4Glc) solutions were prepared using 0.01M sodium chloride solution as a solvent. Aiming at a sugar chain molecule, a porous membrane modified by a polymer is arranged in the middle of a self-made electrochemical tank, sugar chain solutions with the concentration from small to large are sequentially added to two sides of the electrochemical tank, the waiting time is 10min, then electrodes are inserted, and transmembrane ionic current is measured once after each solution addition. The current increase ratio and the concentration increase condition are plotted, as shown in fig. 8, it can be clearly seen that the ion current increase conditions of the nanochannel caused by different sugar chain molecules are obviously different, and by utilizing the different current change characteristics, the polymer modified nanochannel realizes the identification and detection of different sugar chain molecules.
Example 8
A polymer modified organic porous membrane material is prepared by taking a PEI-g-Gls modified PET membrane as an example, adding the membrane between two self-made electrochemical tanks, filling 0.1M sodium chloride solution on two sides, inserting an electrode (taking a platinum wire electrode as an example here), measuring transmembrane ionic current by using a picoammeter, and measuring the voltage range of the transmembrane ionic current to be-2V. The transmembrane current change before and after the polymer modification is shown in fig. 9, and it can be seen that the transmembrane ionic current of the PET film is reduced due to the reduction of the effective pore size of the nanochannel after the polymer modification.
Example 9
0.1M sodium chloride solution is used as solvent, and the respective preparation concentrations are 1 × 10-8Sialic acid of M, 2,3 trisaccharide chain and 2,6 trisaccharide chain solution. For a sugar molecule, a piece of polymer modified PET membrane is added and held in the middle of a self-made electrochemical cell, the sugar solution is added at two sides of the electrochemical cell, the time is waited for 10min, then a platinum wire electrode is inserted, and transmembrane ionic current is measured.
The current change diagram is shown in fig. 10, and it can be clearly seen that the increase conditions of the ion current of the nanochannel caused by different sugar molecules are significantly different, and by utilizing the different current change characteristics, the polymer modified nanochannel can realize the identification, detection and distinction of different sugar chain molecules under extremely low concentration.
The present invention is not limited to the above-described embodiments, and any obvious improvements, substitutions or modifications can be made by those skilled in the art without departing from the spirit of the present invention.
Claims (9)
1. A functional porous membrane material characterized by: the porous membrane material comprises a porous membrane and a functional polymer grafted to the inner surface of the pore channel of the porous membrane, and the molecular structure of the polymer is as follows:
wherein the grafting amount is 1-99% (based on the number of primary amino groups), and n is 20-120;
the porous membrane is an inorganic porous membrane or an organic porous membrane.
2. The functional porous membrane material of claim 1, wherein: the inorganic porous membrane is one of an anodic aluminum oxide porous membrane and a silicon nitride porous membrane.
3. The functional porous membrane material of claim 1, wherein: the organic porous membrane is one of polyethylene terephthalate, polyimide and polycarbonate porous membrane.
4. A method of preparing a functional porous membrane material according to any one of claims 1 to 3, characterized in that: the preparation method comprises the following steps of grafting functional recognition unit-glucoside molecules on a polymer framework, and grafting a polymer with a recognition unit to the inner surface of a pore channel of a porous membrane, wherein the specific steps are as follows:
(1) dissolving 1g of polyethyleneimine (branched) in 20ml of ethanol to prepare a polymer solution; weighing 4-formylphenyl-beta-D-glucoside 0.5-1.5 g, adding into the polymer solution, and reacting at room temperature for 24 hours; then putting the reaction solution into a dialysis bag, dialyzing in water for 5 days, and freeze-drying to obtain the required polymer;
(2) modifying the inorganic porous membrane: placing the inorganic porous membrane modified with isothiocyanate groups into a flask, adding 10mL of methanol/water solution (v/v is 1/1) dissolved with 0.2g of polymer, and reacting at 60 ℃ for 24 h;
(3) modifying the organic porous membrane: placing the chemically etched organic porous membrane into a flask; after activation with 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS), the resulting mixture was immersed in a polymer solution (20mg/mL) and reacted at room temperature for 24 hours.
5. Use of the functional porous membrane material according to claim 1 for the recognition of complex sugar chain molecules, characterized in that: the porous membrane material is applied to high-sensitivity detection of monosaccharides and sugar chains.
6. The use of the functional porous membrane material according to claim 5 in the recognition of complex sugar chain molecules, characterized in that: the specific steps of the porous membrane material in the high-sensitivity detection application of monosaccharide and sugar chain are as follows:
step 1, placing a porous membrane modified by a polymer between electrochemical cell clamps, then injecting an electrolyte into the cell, standing for 5-15 minutes, inserting electrodes at two ends of the electrochemical cell, and measuring transmembrane current of the electrochemical cell by using a picoammeter;
and 2, removing the electrolyte added in the step 1, adding the electrolyte containing different types of sugar molecules with different concentrations again, standing for 5-15 minutes, inserting electrodes at two ends of the electrochemical cell, and measuring transmembrane current of the electrochemical cell by using a Peak meter.
7. The use of the functional porous membrane material according to claim 6 in the recognition of complex sugar chain molecules, characterized in that: the electrolyte is 0.01-2.0 mol/L sodium chloride or potassium chloride solution, the pH of the electrolyte is 2-10, and the solvent is deionized water solution.
8. The use of the functional porous membrane material according to claim 6 in the recognition of complex sugar chain molecules, characterized in that: the electrode is a silver-silver chloride electrode, a mercury-mercury chloride electrode, a graphite or platinum wire electrode.
9. The use of the functional porous membrane material according to claim 6 in the recognition of complex sugar chain molecules, characterized in that: when a picoammeter is used in the step 1 and the step 2 to measure the change of transmembrane ion current, a power supply applies-0.2V scanning voltage to two ends of an electrode, and the duration time of each voltage is 1-2 seconds.
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| CN115541893A (en) * | 2021-06-30 | 2022-12-30 | 无锡博慧斯生物医药科技有限公司 | Saccharification detection card membrane treatment process |
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