CN112147119A - Fluorescent probe device with high-sensitivity detection - Google Patents
Fluorescent probe device with high-sensitivity detection Download PDFInfo
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- CN112147119A CN112147119A CN202011051350.2A CN202011051350A CN112147119A CN 112147119 A CN112147119 A CN 112147119A CN 202011051350 A CN202011051350 A CN 202011051350A CN 112147119 A CN112147119 A CN 112147119A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pathology (AREA)
- Optics & Photonics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a fluorescent probe device with high-sensitivity detection, which comprises an analyzer main body, wherein an excitation structure, a detection structure and an auxiliary structure are arranged in the analyzer main body, the detection structure is positioned in the analyzer main body, the excitation structure is positioned on the inner wall surface of the analyzer main body, the excitation structure is wrapped on the detection structure, and the auxiliary structure is positioned in the detection structure; in the above, the detecting structure mainly comprises a first slide rail, a carrier, a first light source and a microscope; the present invention relates to the field of chemical detection. The device integrated design, the structure is scientific and compact, can satisfy the light intensity of different degrees, the fluorescence reaction under the different chromatogram's the light of closing to can also satisfy the temperature needs that need low temperature or high temperature detection because of special medicament, provide diversified detection environment, guarantee the accuracy of detection, the sensitivity of probe.
Description
Technical Field
The invention relates to the field of chemical detection, in particular to a fluorescent probe device with high-sensitivity detection.
Background
The fluorescence detection is a natural luminescence reaction, human cells, bacteria, mould, food residues and the like can be detected by reacting luciferase with ATP, the illumination intensity is measured by special equipment and is expressed in a digital form, was first applied in 1975 to the food industry, was most commonly used in the cosmetic manufacturing industry in 1985 for labeling antigens or antibodies in the fluoroimmunoassay, and also in the microenvironment, for example, the detection of microscopic characteristics of surfactant micelles, bimolecular membranes, protein active sites and the like generally requires a probe with a large molar absorption coefficient and a high fluorescence quantum yield; the fluorescence emission wavelength is in long wave and has larger Stokes shift; when the reagent is used for the immunoassay, binding to antigens or antibodies should not affect their activity and can also be used to label the nucleotide fragments to be detected for specific, quantitative detection of the amount of nucleic acids.
Disclosure of Invention
In view of the shortcomings of the prior art, the present invention provides a fluorescent probe device with high sensitivity detection. The method solves the problems that the detection sensitivity of the probe is insufficient when the fluorescent probe is used for detection, the detection result is inaccurate due to the influence of the surrounding environment, and the analysis comparison test is complex.
In order to achieve the purpose, the invention is realized by the following technical scheme: the analyzer comprises an analyzer main body, wherein an excitation structure, a detection structure and an auxiliary structure are arranged in the analyzer main body, the detection structure is positioned in the analyzer main body, the excitation structure is positioned on the inner wall surface of the analyzer main body, the excitation structure is wrapped on the detection structure, and the auxiliary structure is positioned in the detection structure;
in the above, the detecting structure mainly comprises a first slide rail, a carrier, a first light source and a microscope;
the analyzer comprises an analyzer body, a microscope, a light source, a slide rail, a carrier, a microscope, a light source and a light source, wherein the analyzer body is provided with a first rectangular through hole at the lower end of the left wall surface, the first slide rail is positioned on the lower wall surface of the rectangular through hole, the carrier is positioned on the slide rail and is positioned at the center of the analyzer body, the upper wall surface of the analyzer body is provided with a second rectangular through hole, the microscope is positioned in the second rectangular through hole and is positioned right above the carrier, and the first light source is positioned on the.
Preferably, the excitation structure mainly comprises: the second light source, the first tight closing valve, the second tight closing valve and the sealing plate;
the sealing plate is distributed on the inner wall surface of the analyzer main body, the first tight closing valve is located on the right wall surface of the analyzer main body, the second tight closing valve is located on the right wall surface of the analyzer main body, the left end of the first tight closing valve of the second tight closing valve is located at the left end of the second tight closing valve, the sealing plate is installed on the inner wall surface of the analyzer main body, the sealing plate forms a darkroom, and the second light source is installed on the inner wall surface of the darkroom formed by the sealing plate.
Preferably, the auxiliary structure mainly comprises: the device comprises a first gear, a rack, a threaded pipe, a first support column, a second support column and a third support column;
the rack is fixed on the lower wall surface of the carrier, the rack is positioned above the first gear, the screwed pipe is positioned at the left end of the carrier base, the screwed pipe wraps the first support, the third support is positioned at the right end of the screwed pipe, and the third support is connected with the carrier base.
Preferably, a first enlarged disc is installed at the left end of the first support, a second enlarged disc is installed at the left end of the threaded pipe, and the second enlarged disc is sleeved and embedded in the first enlarged disc.
Preferably, a vortex tube is installed on the right wall surface of the analyzer body, and a cold port and a hot port of the vortex tube are respectively connected to the first tight closing valve and the second tight closing valve.
Preferably, the back surface of the sealing plate is provided with a plurality of heat conducting strips.
Advantageous effects
The device integrated design, the structure is scientific and compact, can satisfy the light intensity of different degrees, the fluorescence reaction under the different chromatogram's the light of closing to can also satisfy the temperature needs that need low temperature or high temperature detection because of special medicament, provide diversified detection environment, guarantee the accuracy of detection, the sensitivity of probe.
Drawings
FIG. 1 is a schematic structural diagram of a fluorescent probe device with high sensitivity detection according to the present invention.
FIG. 2 is a top view of a fluorescent probe device with high sensitivity detection according to the present invention.
FIG. 3 is a left side view of a fluorescent probe device with high sensitivity detection according to the present invention.
In the figure: 1-an analyzer body; 2-a first slide rail; 3-a carrier; 4-a first light source; 5-a microscope lens; 6-a second light source; 7-a first tight closing valve; 8-a second tight closing valve; 9-sealing plate; 10-a first gear; 11-a rack; 12-a threaded pipe; 13-a first pillar; 14-a second strut; 15-a third strut; 16-a first enlarged disc; 17-a second enlarged disc; 18-vortex tube.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-3, the present invention provides a technical solution: in order to achieve the purpose, the invention is realized by the following technical scheme: the analyzer comprises an analyzer main body 1, wherein an excitation structure, a detection structure and an auxiliary structure are arranged in the analyzer main body 1, the detection structure is positioned in the analyzer main body 1, the excitation structure is positioned on the inner wall surface of the analyzer main body 1, the excitation structure is wrapped on the detection structure, and the auxiliary structure is positioned in the detection structure; in the above, the detecting structure mainly comprises a first slide rail 2, a carrier rack 3, a first light source 4 and a microscope 5; the lower end of the left wall surface of the analyzer main body 1 is provided with a first rectangular through hole, the first slide rail 2 is positioned on the lower wall surface of the rectangular through hole, the object carrier 3 is positioned on the slide rail, the object carrier 3 is positioned at the center of the analyzer main body 1, the upper wall surface of the analyzer main body 1 is provided with a second rectangular through hole, the microscope 5 is positioned in the second rectangular through hole, the microscope 5 is positioned above the object carrier 3, and the first light source 4 is positioned on the object carrier 3; the excitation structure mainly comprises: a second light source 6, a first tight closing valve 7, a second tight closing valve 8 and a sealing plate 9; the sealing plate 9 is distributed on the inner wall surface of the analyzer main body 1, the first tight closing valve 7 is positioned on the right wall surface of the analyzer main body 1, the second tight closing valve 8 is positioned at the left end of the first tight closing valve 7, the sealing plate 9 is installed on the inner wall surface of the analyzer main body 1, the sealing plate 9 forms a darkroom, and the second light source 6 is installed on the inner wall surface of the darkroom formed by the sealing plate 9; the auxiliary structure mainly comprises: a first gear 10, a rack 11, a threaded pipe 12, a first support 13, a second support 14, and a third support 15; the first support 13 is located at the left end of the base of the carrier 3, the second support 14 is located at the right end of the first support 13, the first gear 10 is located at the right end of the second support 14, the first gear 10 is located below the carrier 3, the rack 11 is fixed on the lower wall surface of the carrier 3, the rack 11 is located above the first gear 10, the threaded pipe 12 is located at the left end of the base of the carrier 3, the threaded pipe 12 is wrapped on the first support 13, the third support 15 is located at the right end of the threaded pipe 12, and the third support 15 is connected to the base of the carrier 3; a first enlarged disc 16 is arranged at the left end of the first support 13, a second enlarged disc 17 is arranged at the left end of the threaded pipe 12, and the second enlarged disc 17 is sleeved on the first enlarged disc 16; a vortex tube 18 is arranged on the right wall surface of the analyzer body 1, and cold and hot ports of the vortex tube 18 are respectively connected with the first close valve 7 and the second close valve 8; the back of the sealing plate 9 is provided with a plurality of heat conducting strips.
Example (b): the detailed connection means is the known technology in the field, and the following working principle is not described, but the working principle and process are mainly described, when in use, please refer to fig. 1-3, the analyzer main body 1 is set up, the first slide rail 2 slides leftwards to expose the carrier 3, the probe and the sample are put into the carrier 3, the first slide rail 2 moves rightwards to send the sample into the device, the first light source 4 is opened, the microscope 5 is observed, the first enlarged disc 16 is rotated to drive the first support 13, the first support 13 drives the second support 14, the second support 14 drives the first gear 10, the first gear 10 drives the rack 11 to drive the carrier 3 to move back and forth, the second enlarged disc 17 is rotated, the second enlarged disc 17 drives the spiral tube 12, the spiral tube 12 rotates, the third support 15 is extended and retracted, the third support 15 drives the carrier 3 to move left and right, the position is adjusted to enable the sample to enter the visual field of the microscope 5, the microscope 5 and the first light source 4 are adjusted to enable the visual field to be clear and complete, if necessary, the first close valve 7 can be opened, the air flow is divided by the vortex tube 18, cold air is input through the first close valve 7, or the second tight closing valve 8 is opened, the airflow is divided by the vortex tube 18, hot air is input through the second tight closing valve 8, the environment temperature required by the test is ensured, the second light source 6 positioned on the sealing plate 9 can be opened, the proper brightness and different light colors are adjusted, and different light environments are constructed, so that the comparison test is conveniently carried out.
As a preferred scheme, furthermore, the sealing plate 9 is distributed on the inner wall surface of the analyzer body 1, the first close valve 7 is located on the right wall surface of the analyzer body 1, the second close valve 8 is located at the left end of the first close valve 7, the sealing plate 9 is installed on the inner wall surface of the analyzer body 1, the sealing plate 9 forms a darkroom, and the second light source 6 is installed on the inner wall surface of the darkroom formed by the sealing plate 9.
Preferably, the first support 13 is located at the left end of the base of the object carrier 3, the second support 14 is located at the right end of the first support 13, the first gear 10 is located at the right end of the second support 14, the first gear 10 is located below the object carrier 3, the rack 11 is fixed on the lower wall surface of the object carrier 3, the rack 11 is located above the first gear 10, the threaded pipe 12 is located at the left end of the base of the object carrier 3, the threaded pipe 12 wraps the first support 13, the third support 15 is located at the right end of the threaded pipe 12, and the third support 15 is connected to the base of the object carrier 3.
Preferably, a first enlarged disc 16 is mounted at the left end of the first support 13, a second enlarged disc 17 is mounted at the left end of the threaded pipe 12, and the second enlarged disc 17 is sleeved on the first enlarged disc 16.
Preferably, a further vortex tube 18 is located on the right wall surface of the analyzer body 1, and cold and hot ports of the vortex tube 18 are connected to the first and second tightly closed valves 7 and 8, respectively.
Preferably, the back surface of the sealing plate 9 is provided with a plurality of heat conducting strips.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation. The use of the phrase "comprising one of the elements does not exclude the presence of other like elements in the process, method, article, or apparatus that comprises the element.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. A fluorescent probe device with high-sensitivity detection comprises an analyzer main body (1), and is characterized in that an excitation structure, a detection structure and an auxiliary structure are installed in the analyzer main body (1), the detection structure is located in the analyzer main body (1), the excitation structure is located on the inner wall surface of the analyzer main body (1), the excitation structure is wrapped in the detection structure, and the auxiliary structure is located in the detection structure;
the detection structure mainly comprises a first slide rail (2), a carrier (3), a first light source (4) and a microscope (5);
the analyzer comprises an analyzer main body (1), a first rectangular through hole is formed in the lower end of the left wall face of the analyzer main body (1), a first sliding rail (2) is located on the lower wall face of the rectangular through hole, a carrier (3) is located on the sliding rail and is located at the center of the analyzer main body (1), a second rectangular through hole is formed in the upper wall face of the analyzer main body (1), a microscope (5) is located in the second rectangular through hole, the microscope (5) is located above the carrier (3), and a first light source (4) is located on the carrier (3).
2. The fluorescent probe device with high sensitivity detection according to claim 1, wherein the excitation structure mainly comprises: a second light source (6), a first tight closing valve (7), a second tight closing valve (8) and a sealing plate (9);
the utility model discloses a closed light source, including closing plate (9), first tight valve (7) and second tight valve (8), closing plate (9) distributes on analysis appearance main part (1) internal face, first tight valve (7) are located analysis appearance main part (1) right side wall, second tight valve (8) are located analysis appearance main part (1) right side wall, just second tight valve (8) are located first tight valve (7) left end, closing plate (9) are installed on analysis appearance main part (1) internal face, just closing plate (9) constitute the darkroom, second light source (6) are installed on closing plate (9) constitute the darkroom internal face.
3. The fluorescent probe device with high sensitivity detection according to claim 1, wherein the auxiliary structure mainly comprises: a first gear (10), a rack (11), a threaded pipe (12), a first strut (13), a second strut (14) and a third strut (15);
the rack is characterized in that the first support column (13) is located at the left end of a base of the object carrier (3), the second support column (14) is located at the right end of the first support column (13), the first gear (10) is located at the right end of the second support column (14), the first gear (10) is located below the object carrier (3), the rack (11) is fixed on the lower wall surface of the object carrier (3), the rack (11) is located above the first gear (10), the threaded pipe (12) is located at the left end of the base of the object carrier (3), the threaded pipe (12) wraps the first support column (13), the third support column (15) is located at the right end of the threaded pipe (12), and the third support column (15) is connected to the base of the object carrier (3).
4. The fluorescence probe device with high sensitivity detection of claim 3, wherein the first column (13) is provided with a first enlarged disk (16) at the left end, the threaded pipe (12) is provided with a second enlarged disk (17) at the left end, and the second enlarged disk (17) is sleeved on the first enlarged disk (16).
5. The fluorescent probe device with high sensitivity detection according to claim 1, characterized in that a vortex tube (18) is installed on the right wall surface of the analyzer body (1), and the cold and hot ports of the vortex tube (18) are respectively connected to the first close-closing valve (7) and the second close-closing valve (8).
6. The fluorescent probe device with high sensitivity detection according to claim 2, characterized in that the back of the sealing plate (9) is provided with a plurality of heat conducting strips.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011051350.2A CN112147119A (en) | 2020-09-29 | 2020-09-29 | Fluorescent probe device with high-sensitivity detection |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011051350.2A CN112147119A (en) | 2020-09-29 | 2020-09-29 | Fluorescent probe device with high-sensitivity detection |
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| Publication Number | Publication Date |
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| CN112147119A true CN112147119A (en) | 2020-12-29 |
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| CN202011051350.2A Withdrawn CN112147119A (en) | 2020-09-29 | 2020-09-29 | Fluorescent probe device with high-sensitivity detection |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117470824A (en) * | 2023-12-27 | 2024-01-30 | 西安石油大学 | Fluorescent probe device with high-sensitivity detection |
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2020
- 2020-09-29 CN CN202011051350.2A patent/CN112147119A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117470824A (en) * | 2023-12-27 | 2024-01-30 | 西安石油大学 | Fluorescent probe device with high-sensitivity detection |
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Application publication date: 20201229 |