CN112141505B

CN112141505B - DNA extraction kit

Info

Publication number: CN112141505B
Application number: CN202011027429.1A
Authority: CN
Inventors: 杨磊; 申恒杰
Original assignee: Chongqing Wenchuang Medical Laboratory Co ltd
Current assignee: Chongqing Wenchuang medical laboratory Co.,Ltd.
Priority date: 2020-09-25
Filing date: 2020-09-25
Publication date: 2022-02-11
Anticipated expiration: 2040-09-25
Also published as: CN112141505A

Abstract

The invention relates to the field of kits, in particular to a DNA extraction kit, which comprises a box body and a box cover for covering the box body; a kit containing groove is formed in the box body, a plurality of kit containing positions are formed in the bottom of the kit containing groove, the DNA extraction kit further comprises a fixing plate, the fixing plate is hinged with the kit containing groove, and a plurality of through holes are formed in the positions, corresponding to the kit containing positions, of the fixing plate; the fixing plate is also provided with a mounting hole, and the opening of the accommodating groove is also provided with a clamping piece which comprises a vertical section and a horizontal section; the box cover comprises a top end and a bottom end opposite to the top end, the bottom end is hinged with the box body, a buckle is arranged in the middle of the top end, and a clamping groove is formed in the box body corresponding to the buckle; when the box cover is closed, the buckle is pressed down to stretch into the clamping groove, so that the box cover is locked. The DNA extraction kit has the advantages of firm installation and capability of ensuring that the test tube cannot topple over in the transportation process to cause leakage of the solution to be detected.

Description

DNA extraction kit

Technical Field

The invention relates to the field of kits, in particular to a DNA extraction kit.

Background

DNA is a carrier of genetic information, is one of the main subjects of molecular biology research, and with the continuous deepening of molecular biology research and the rapid development of gene sequencing technology, the time and cost required for gene sequencing are rapidly reduced, and diagnosis of diseases at the gene level has become a basic technical means of clinical diagnosis, and accurate medical treatment based on gene sequencing is gradually becoming an important mode in the fields of major disease diagnosis, personalized medical treatment, fetal genetic disease analysis, individual identification, and the like. When DNA extraction is required to be detected, a solution to be detected is placed into a test tube and then is placed into a DNA kit, but the existing DNA kit is not firm in mounting the test tube, and the solution to be detected is often toppled or collided in the transportation process, so that the solution to be detected is leaked.

In view of the above, there is a need to provide a novel DNA extraction kit to solve or at least alleviate the above technical drawbacks.

Disclosure of Invention

The invention mainly aims to provide a DNA extraction kit, and aims to solve the technical problems that an existing DNA extraction kit is not firm in mounting a test tube, and liquid to be detected in the test tube is easy to leak.

In order to achieve the above object, the present invention provides a DNA extraction kit comprising:

the box body and the box cover the box body, and the box cover is hinged with the box body to open or close the box body;

the kit containing groove is formed in the box body, a plurality of kit containing positions are formed in the bottom of the kit containing groove, the DNA extraction kit further comprises a fixing plate, the fixing plate is hinged with the kit containing groove, and a plurality of through holes are formed in the positions, corresponding to the kit containing positions, of the fixing plate;

the fixing plate is further provided with a mounting hole, a clamping piece is further arranged at an opening of the kit containing groove, the clamping piece comprises a vertical section and a horizontal section, the vertical section is vertically mounted on the box body, and one end of the horizontal section is hinged with the vertical section so that the horizontal section can rotate around the hinged point in a plane perpendicular to the vertical section;

the size of the mounting hole is larger than that of the horizontal section, when the fixing plate is put down, the horizontal section penetrates through the mounting hole, and then the horizontal section is rotated to clamp the fixing plate between the box body and the horizontal section;

the box cover comprises a top end and a bottom end opposite to the top end, the bottom end is hinged with the box body, a buckle is arranged at the middle position of the top end, and a clamping groove is arranged at the position, corresponding to the buckle, of the box body; when the box cover is closed, the buckle is pressed down to extend into the clamping groove, so that the box cover is locked.

Preferably, the inner wall of the box body is further provided with a horizontal detection device, the horizontal detection device comprises a vertically arranged U-shaped pipe, conductive liquid is arranged in the U-shaped pipe, two openings of the U-shaped pipe are respectively provided with an alarm device, the alarm device comprises a battery, a contact electrode and an alarm, the top surface of the battery is connected with the bottom surface of the alarm, the bottom surface of the battery is connected with the top surface of the contact electrode, and a gap is reserved between the bottom surface of the contact electrode and the conductive liquid; when the inclination angle of the U-shaped pipe is larger than a preset value, the conductive liquid is in contact with the contact electrode arranged at one end of the U-shaped pipe, and therefore an alarm circuit is switched on.

Preferably, the kit further comprises a pressure-sensitive sensor, a controller and an LED lamp, wherein the controller is in signal connection with the pressure-sensitive sensor and the LED lamp respectively, an opening is formed at a position of the box body facing the box cover, the pressure-sensitive sensor is arranged on a side edge of the opening, and the LED lamp is arranged on the box cover; when the box cover and the box body are closed and opened, the pressure-sensitive sensor detects a pressure reduction signal and then sends a light-on signal to the controller, and the controller controls the LED lamp to be turned on; when the box cover and the box body are closed, the pressure-sensitive sensor detects a pressure increase signal and then sends a light turning-off signal to the controller, and the controller controls the LED lamp to be turned off.

Preferably, a pulling plate is further arranged below the reagent kit accommodating groove, a handle connected with the pulling plate is arranged on the box body, vertical rods used for supporting the reagent kit accommodating groove are further arranged on two sides of the pulling plate, and the pulling plate is slidably arranged on the box body.

Preferably, a sliding groove is formed in the bottom of the drawing plate, a sliding rail is arranged at a position, corresponding to the sliding groove, of the box body, and the drawing plate is detachably mounted on the box body.

Preferably, one surface of the drawing plate facing the reagent kit accommodating groove is provided with an elastic block, and the side surface of the elastic block is an inclined surface.

Preferably, the bottom of box body is provided with the supporting legs, the supporting legs include bracing piece, callus on the sole and suit in the spring of bracing piece, the callus on the sole set up in the bottom of bracing piece.

Preferably, still be provided with the rubber seat in holding the box mounting groove, be provided with on the rubber seat the kit places the position.

Preferably, a refrigerating groove is further formed in the box body, a refrigerator is arranged in the refrigerating groove, and a heat insulation plate is arranged between the refrigerating groove and the kit mounting groove.

Preferably, the number of the reagent cartridge placing sites is plural.

In the above technical scheme of the present invention, the DNA extraction kit comprises: the box body and the box cover the box body, and the box cover is hinged with the box body to open or close the box body; a kit containing groove is formed in the box body, a plurality of kit containing positions are formed in the bottom of the kit containing groove, the DNA extraction kit further comprises a fixing plate, the fixing plate is hinged with the kit containing groove, and a plurality of through holes are formed in the positions, corresponding to the kit containing positions, of the fixing plate; the fixing plate is also provided with a mounting hole, the opening of the accommodating groove is also provided with a clamping piece, the clamping piece comprises a vertical section and a horizontal section, the vertical section is vertically arranged on the box body, and one end of the horizontal section is hinged with the vertical section so that the horizontal section can rotate around a hinged point in a plane perpendicular to the vertical section; the size of the mounting hole is larger than that of the horizontal section, when the fixing plate is put down, the horizontal section penetrates through the mounting hole, and then the horizontal section is rotated to clamp the fixing plate between the box body and the horizontal section; the box cover comprises a top end and a bottom end opposite to the top end, the bottom end is hinged with the box body, a buckle is arranged at the middle position of the top end, and a clamping groove is arranged at the position of the box body corresponding to the buckle; when the box cover is closed, the buckle is pressed down to stretch into the clamping groove, so that the box cover is locked. Among the above-mentioned scheme, when the test tube that is equipped with the detection liquid that waits to detect DNA is placed to needs, put down the fixed plate earlier, insert the test tube from the through-hole of fixed plate on the kit places the position, fix the test tube through the bottom and the middle part of test tube to thereby rotate the horizontal segment through joint spare with the fixed plate chucking between box body and horizontal segment, ensure that the fixed plate installation is firm, and then guarantee that the DNA kit can not empty in the transportation. And, through the cooperation of buckle and draw-in groove, guarantee that the lid is connected with the box body firmly. The test tube detection device has the advantages of being firm in installation and capable of ensuring that the test tube cannot topple over to cause leakage of liquid to be detected in the transportation process.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.

FIG. 1 is a schematic perspective view of a DNA extraction kit according to an embodiment of the present invention;

FIG. 2 is a schematic perspective view of a case according to an embodiment of the present invention;

FIG. 3 is a sectional view of a DNA extraction kit according to an embodiment of the present invention;

FIG. 4 is a schematic perspective view of a connector according to an embodiment of the present invention;

fig. 5 is a schematic view of a horizontal detection device according to an embodiment of the invention.

The reference numbers illustrate:

the implementation, functional features and advantages of the present invention will be further explained with reference to the accompanying drawings.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.

It should be noted that all the directional indicators (such as the upper and lower … …) in the embodiment of the present invention are only used to explain the relative position relationship, movement, etc. of the components in a specific posture (as shown in the drawings), and if the specific posture is changed, the directional indicator is changed accordingly.

In addition, the descriptions related to "first", "second", etc. in the present invention are only for descriptive purposes and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature.

Moreover, the technical solutions in the embodiments of the present invention may be combined with each other, but it is necessary to be able to be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should be considered to be absent, and is not within the protection scope of the present invention.

Referring to fig. 1 to 5, according to a preferred embodiment of the present invention, the present invention provides a DNA extraction kit including:

the box body 11 and a box cover 12 covering the box body 11, wherein the box cover 12 is hinged with the box body 11 to open or close the box body 11;

the kit containing groove 2 is formed in the box body 11, a plurality of kit placing positions 21 are formed in the bottom of the kit containing groove 2, the DNA extraction kit further comprises a fixing plate 3, the fixing plate 3 is hinged to the kit containing groove 2, and a plurality of through holes 31 are formed in the positions, corresponding to the kit placing positions 21, of the fixing plate 3;

the fixing plate 3 is further provided with a mounting hole 32, the opening of the accommodating groove is further provided with a clamping piece, the clamping piece comprises a vertical section 41 and a horizontal section 42, the vertical section 41 is vertically arranged on the box body 11, and one end of the horizontal section 42 is hinged with the vertical section 41 so that the horizontal section 42 can rotate around a hinged point in a plane perpendicular to the vertical section 41;

the size of the mounting hole 32 is larger than that of the horizontal section 42, and when the fastening plate 3 is lowered, the horizontal section 42 passes through the mounting hole 32, and then the horizontal section 42 is rotated to clamp the fastening plate 3 between the box body 11 and the horizontal section 42;

the box cover 12 comprises a top end and a bottom end opposite to the top end, the bottom end is hinged with the box body 11, a buckle 5 is arranged in the middle of the top end, and a clamping groove 6 is arranged in the position, corresponding to the buckle 5, of the box body 11; when the box is closed, the buckle 5 is pressed down to extend into the clamping groove 6, so that the box cover 12 is locked.

Above-mentioned embodiment, when the test tube that is equipped with the detection liquid that waits to detect DNA is placed to needs, put down fixed plate 3 earlier, insert the test tube from fixed plate 3's through-hole 31 on the position 21 is placed to the kit, fix the test tube through the bottom and the middle part of test tube to thereby rotate horizontal segment 42 through joint spare with fixed plate 3 chucking between box body 11 and horizontal segment 42, ensure that fixed plate 3 installation is firm, and then guarantee that the DNA kit can not topple over in the transportation. And, through the cooperation of buckle 5 with draw-in groove 6, guarantee that lid 12 is connected with box body 11 firmly. This embodiment has the installation firmly, can ensure that the test tube can not topple over and cause to detect the advantage that the liquid was revealed in the transportation.

According to the preferred embodiment of the invention, the inner wall of the box body 11 is further provided with a horizontal detection device, the horizontal detection device comprises a vertically arranged U-shaped pipe 71, a conductive liquid 72 is arranged in the U-shaped pipe, two openings of the U-shaped pipe 71 are respectively provided with an alarm device 8, each alarm device 8 comprises a battery 81, a contact electrode 83 and an alarm 82, the top surface of the battery 81 is connected with the bottom surface of the alarm 82, the bottom surface of the battery 81 is connected with the top surface of the contact electrode 83, and a gap is reserved between the bottom surface of the contact electrode 83 and the conductive liquid 72; when the inclination angle of the U-shaped pipe 71 is larger than the preset value, the conductive liquid 72 is in contact with the contact electrode 83 arranged at one end of the U-shaped pipe 71, so that the alarm circuit is conducted. When 11 inclination of box body is greater than the default, then the liquid that awaits measuring of test tube has the risk of pouring out, can send out the police dispatch newspaper through level detection device, suggestion operator correct operation. The preset value can be 10-20 degrees, and can be set by a person skilled in the art according to actual needs.

Further, the kit also comprises a pressure-sensitive sensor 9, a controller 10 and an LED lamp 20, wherein the controller 10 is in signal connection with the pressure-sensitive sensor 9 and the LED lamp 20 respectively, an opening is formed at the position, facing the box cover 12, of the box body 11, the pressure-sensitive sensor 9 is arranged on the side edge of the opening, and the LED lamp 20 is arranged on the box cover 12; when the box cover 12 and the box body 11 are closed and opened, the pressure-sensitive sensor 9 detects a pressure reduction signal and then sends a light-on signal to the controller 10, and the controller 10 controls the LED lamp 20 to be turned on; when the box cover 12 and the box body 11 are closed, the pressure-sensitive sensor 9 detects a pressure increase signal and then sends a light-off signal to the controller 10, and the controller 10 controls the LED lamp 20 to be turned off. The on-off of the LED lamp 20 can be automatically controlled through the pressure-sensitive sensor 9 and the controller 10, and the LED lamp 20 is arranged so that an operator can clearly see labels of the test tubes and other articles in the box body 11.

Further, a drawing plate 22 is further arranged below the reagent kit accommodating groove 2, a handle connected with the drawing plate 22 is arranged on the box body 11, vertical rods 30 used for supporting the reagent kit accommodating groove 2 are further arranged on two sides of the drawing plate 22, and the drawing plate 22 is slidably mounted on the box body 11. The bottom of the drawing plate 22 is provided with a sliding groove, the position of the box body 11 corresponding to the sliding groove is provided with a sliding rail, and the drawing plate 22 is detachably installed on the box body 11. The drawer plate 22 may be provided with a storage box for storing the desired items. And when the drawing plate 22 is taken out, the reagent vessel 2 can be pushed out from the bottom of the reagent vessel 2 for cleaning or replacement.

Further, one surface of the drawing plate 22 facing the reagent kit accommodating groove 2 is provided with an elastic block 221, and the side surface of the elastic block 221 is an inclined surface. The inclined plane mainly plays a guiding role, and when the drawing plate 22 needs to be placed into the box body 11, the drawing plate is more conveniently placed through the guiding function of the inclined plane.

Further, the bottom of box body 11 is provided with supporting legs 23, and supporting legs 23 includes bracing piece, callus on the sole and the spring of suit in the bracing piece, and the callus on the sole sets up in the bottom of bracing piece. The spring has a buffering effect, and the shaking of the DNA extraction kit caused by the vibration of the table top is reduced. The callus on the sole can guarantee that box body 11 is more firm to be placed on the mesa.

Further, still be provided with the rubber seat in holding the box mounting groove, be provided with the kit on the rubber seat and place position 21. The rubber has certain elasticity and can play a certain buffering role.

Further, a refrigerating tank 24 is arranged in the box body 11, a refrigerator is arranged in the refrigerating tank 24, and a heat insulation plate 25 is arranged between the refrigerating tank 24 and the reagent kit mounting groove. In practice, some of the liquid to be tested has a storage temperature requirement, and can be placed in the refrigerating tank 24

Further, the number of the reagent cartridge placing positions 21 is plural, and the shape and the size of the reagent cartridge placing positions 21 can be set according to actual needs.

The above description is only a preferred embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications and equivalents of the technical spirit of the present invention, which are made by the contents of the present specification and the accompanying drawings, or directly/indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims

1. A DNA extraction kit, comprising:

the box comprises a box body (11) and a box cover (12) covering the box body (11), wherein the box cover (12) is hinged with the box body (11) to open or close the box body (11);

the kit containing groove (2) is formed in the box body (11), a plurality of kit placing positions (21) are arranged at the bottom of the kit containing groove (2), the DNA extraction kit further comprises a fixing plate (3), the fixing plate (3) is hinged to the kit containing groove (2), and a plurality of through holes (31) are formed in the position, corresponding to the kit placing positions (21), of the fixing plate (3);

the fixing plate (3) is further provided with a mounting hole (32), a clamping piece is further arranged at an opening of the kit accommodating groove (2), the clamping piece comprises a vertical section (41) and a horizontal section (42), the vertical section (41) is vertically arranged on the box body (11), and one end of the horizontal section (42) is hinged to the vertical section (41) so that the horizontal section (42) can rotate around the hinge point in a plane perpendicular to the vertical section (41);

the size of the mounting hole (32) is larger than that of the horizontal section (42), when the fixing plate (3) is put down, the horizontal section (42) penetrates through the mounting hole (32), and then the horizontal section (42) is rotated to clamp the fixing plate (3) between the box body (11) and the horizontal section (42);

the box cover (12) comprises a top end and a bottom end opposite to the top end, the bottom end is hinged with the box body (11), a buckle (5) is arranged in the middle of the top end, and a clamping groove (6) is arranged in the position, corresponding to the buckle (5), of the box body (11); when the box is closed, the buckle (5) is pressed to extend into the clamping groove (6) so as to lock the box cover (12);

the inner wall of the box body (11) is further provided with a horizontal detection device, the horizontal detection device comprises a vertically arranged U-shaped pipe (71), conductive liquid (72) is arranged in the U-shaped pipe, two openings of the U-shaped pipe (71) are respectively provided with an alarm device (8), each alarm device (8) comprises a battery (81), a contact electrode (83) and an alarm (82), the top surface of the battery (81) is connected with the bottom surface of the alarm (82), the bottom surface of the battery (81) is connected with the top surface of the contact electrode (83), and a gap is reserved between the bottom surface of the contact electrode (83) and the conductive liquid (72); when the inclination angle of the U-shaped pipe (71) is larger than a preset value, the conductive liquid (72) is in contact with the contact electrode (83) arranged at one end of the U-shaped pipe (71), so that an alarm circuit is conducted.

2. The DNA extraction kit according to claim 1, further comprising a pressure sensitive sensor (9), a controller (10) and an LED lamp (20), wherein the controller (10) is in signal connection with the pressure sensitive sensor (9) and the LED lamp (20), respectively, an opening is formed at a position of the case body (11) facing the case cover (12), the pressure sensitive sensor (9) is disposed on a side of the opening, and the LED lamp (20) is disposed on the case cover (12); when the box cover (12) and the box body (11) are closed and opened, the pressure-sensitive sensor (9) detects a pressure reduction signal and then sends a light-on signal to the controller (10), and the controller (10) controls the LED lamp (20) to be turned on; when the box cover (12) and the box body (11) are closed, the pressure-sensitive sensor (9) detects a pressure increase signal and then sends a light-off signal to the controller (10), and the controller (10) controls the LED lamp (20) to be closed.

3. The DNA extraction kit according to claim 1, wherein a drawing plate (22) is further disposed below the kit accommodating groove (2), a handle connected with the drawing plate (22) is disposed on the box body (11), vertical rods (30) for supporting the kit accommodating groove (2) are further disposed on two sides of the drawing plate (22), and the drawing plate (22) is slidably mounted on the box body (11).

4. The DNA extraction kit according to claim 3, wherein a sliding groove is formed at the bottom of the drawing plate (22), a sliding rail is formed at the position of the box body (11) corresponding to the sliding groove, and the drawing plate (22) is detachably mounted on the box body (11).

5. The DNA extraction kit according to claim 4, wherein an elastic block (221) is provided on a surface of the drawing plate (22) facing the kit accommodating groove (2), and a side surface of the elastic block (221) is a slope.

6. The DNA extraction kit according to claim 1, wherein the bottom of the box body (11) is provided with a support leg (23), the support leg (23) comprises a support rod, a foot pad and a spring sleeved on the support rod, and the foot pad is arranged at the bottom of the support rod.

7. The DNA extraction kit according to claim 1, wherein a rubber seat is further provided in the housing box installation groove, and the rubber seat is provided with the kit placement site (21).

8. The DNA extraction kit according to claim 1, wherein a refrigerating tank (24) is further provided in the case body (11), a refrigerator is provided in the refrigerating tank (24), and a heat insulating plate (25) is provided between the refrigerating tank (24) and the kit mounting groove.

9. The DNA extraction kit according to claim 1, wherein the number of the kit placing positions (21) is plural.