CN112080410A - Microorganism detection system and method - Google Patents
Microorganism detection system and method Download PDFInfo
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Abstract
The invention provides a microorganism detection system and a method. The system comprises: the device comprises a processing assembly, a position detection assembly, a mechanical arm, an imaging assembly and a transmission assembly. Wherein, the processing assembly is respectively connected with the position detection assembly, the mechanical arm and the imaging assembly. The position detection subassembly measures the spatial position information who waits to detect the space, and the processing module is according to spatial position information, confirms the position of sampling point, according to the position control arm motion of sampling point in order to obtain the sample on the sampling point, and the transmission subassembly transmits the sample to the formation of image subassembly, and the formation of image subassembly is enlargied the back and is shot the sample, and the processing module is according to the image that the formation of image subassembly was shot and is obtained, confirms the quantity and the kind of microorganism. According to the system, the determination of the number and the types of the microorganisms in the space to be detected can be automatically completed through the microorganism detection system, manual intervention is not needed in the determination process, the labor cost is saved, the time consumption of the detection process is short, and the microorganism detection efficiency is improved.
Description
Technical Field
The invention relates to the technical field of detection, in particular to a microorganism detection system and a microorganism detection method.
Background
The microorganism is tiny and closely related to human beings, covers various types such as beneficial and harmful, and widely relates to various fields such as food, medicine, industry and agriculture, environmental protection, sports and the like, and different scenes in different fields need to detect the microorganism.
There are many existing methods for detecting microorganisms, for example, a volume measurement method in which the number and the type of microorganisms are determined by measuring the amount of hyphae contained in a certain volume of culture solution to reflect the growth of the microorganisms; weighing dry weight method to test the amount of microorganism in a certain weight by measuring the weight proportion of the microorganism in a certain weight by centrifugation or filtration; the hypha length measuring method measures the growth condition of microorganisms by measuring the growth length of hypha in a certain time, so as to determine the number and the type of the microorganisms; the liquid dilution method is a method in which microorganisms of a certain concentration are diluted and then cultured, thereby measuring the number of microorganisms.
However, the existing microbial detection takes a long time and is inefficient.
Disclosure of Invention
The invention provides a microorganism detection system and a microorganism detection method, which are used for solving the problems of long time consumption and low efficiency of the existing microorganism detection.
In a first aspect, the present invention provides a microbial detection system comprising:
the device comprises a position detection assembly, a processing assembly, a mechanical arm, an imaging assembly and a transmission assembly, wherein the processing assembly is respectively connected with the position detection assembly, the mechanical arm and the imaging assembly;
the position detection assembly is used for measuring the spatial position information of the space to be detected;
the processing component is used for determining the position of a sampling point according to the spatial position information and controlling the mechanical arm to move according to the position of the sampling point so as to obtain a sample on the sampling point;
the transport assembly for transporting the sample to the imaging assembly;
the imaging component is used for shooting the sample after being amplified;
the processing component is also used for determining the number and the type of the microorganisms according to the image shot by the imaging component.
Optionally, the transmission assembly includes: the conveying belt and be used for controlling the power unit of conveying belt motion.
Optionally, the processing component is specifically configured to:
and determining the number of sampling points and the positions of the sampling points according to the spatial position information and the sampling density.
Optionally, one or more stages for placing the sample are further placed on the conveying belt.
Optionally, the position detection assembly includes a first position probe and a second position probe, the first position probe is disposed at the bottom of the position detection assembly body, and the second position probe is disposed on the mechanical arm;
the first position probe is used for measuring horizontal position information in the space to be detected;
and the second position probe is used for measuring the height information in the space to be detected.
Optionally, the sampling end of the mechanical arm is detachably provided with a swab for sampling.
In a second aspect, the present invention provides a microorganism detection method applied to the microorganism detection system according to any one of the first aspect, including:
the position detection component measures spatial position information of a space to be detected and sends the spatial position information to the processing component;
the processing component determines the position of a sampling point according to the spatial position information and sends a motion control signal to the mechanical arm according to the position of the sampling point;
the mechanical arm moves according to the motion control signal to obtain a sample on the sampling point;
the transport assembly transports the sample to the imaging assembly;
the imaging component is used for amplifying the sample and then shooting the sample, and sending the shot image to the processing component;
the processing component determines the number and type of microorganisms from the image.
Optionally, the determining, by the processing component, a position of the sampling point according to the spatial position information includes:
and the processing component determines the number of sampling points and the positions of the sampling points according to the spatial position information and the sampling density.
Optionally, the position detection assembly includes a first position probe and a second position probe, the first position probe is disposed at the bottom of the position detection assembly body, and the second position probe is disposed on the mechanical arm;
the position detection assembly measures spatial position information of a space to be detected, and comprises:
measuring horizontal position information in the space to be detected through the first position probe;
and measuring the height information in the space to be detected through the second position probe.
Optionally, the method further includes:
the processing component receives a data transmission request sent by the terminal equipment;
and sending the number and the types of the microorganisms to the terminal equipment.
The invention provides a microorganism detection system and a method, wherein the microorganism detection system comprises: the device comprises a processing assembly, a position detection assembly, a mechanical arm, an imaging assembly and a transmission assembly. Wherein, the processing assembly is respectively connected with the position detection assembly, the mechanical arm and the imaging assembly. The position detection subassembly measures the spatial position information who waits to detect the space, and the processing module is according to spatial position information, confirms the position of sampling point, according to the position control arm motion of sampling point in order to obtain the sample on the sampling point, and the transmission subassembly transmits the sample to the formation of image subassembly, and the formation of image subassembly is enlargied the back and is shot the sample, and the processing module is according to the image that the formation of image subassembly was shot and is obtained, confirms the quantity and the kind of microorganism. The determination of the number and the types of the microorganisms in the space to be detected can be automatically completed through the microorganism detection system, the manual intervention is not needed in the determination process, the labor cost is saved, the detection can be directly performed after the sampling in the determination process, the detection process is short in time consumption, and the microorganism detection efficiency is improved.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present disclosure and together with the description, serve to explain the principles of the disclosure.
FIG. 1 is a schematic view of a microorganism detection system according to the present invention;
FIG. 2A is a schematic illustration of the number distribution of a microorganism;
FIG. 2B is a diagram showing the distribution of the number and types of microorganisms;
FIG. 3 is a schematic view of another embodiment of the microorganism detection system according to the present invention;
FIG. 4 is a schematic flow chart of a method for detecting microorganisms according to the present invention.
Description of the reference numerals
1: a processing component;
2: a position detection component;
3: a mechanical arm;
4: an imaging assembly;
5: a transmission assembly;
31: a sampling end;
51: and (5) conveying the belt.
Detailed Description
Reference will now be made in detail to the exemplary embodiments, examples of which are illustrated in the accompanying drawings. When the following description refers to the accompanying drawings, like numbers in different drawings represent the same or similar elements unless otherwise indicated. The implementations described in the exemplary embodiments below are not intended to represent all implementations consistent with the present disclosure. Rather, they are merely examples of apparatus and methods consistent with certain aspects of the present disclosure, as detailed in the appended claims.
Microorganisms, which are microorganisms that cannot be observed by the naked eye of an individual, include: bacteria, viruses, fungi, small protists, microscopic algae, and the like.
One of the most important effects of microorganisms on humans is the prevalence that leads to infectious diseases. In human diseases 50% are caused by viruses. Microorganisms have led to the history of human disease, i.e., the history of human struggling with it. In the prevention and treatment of diseases, human beings have made great progress, but new and recurring microbial infections continue to occur, like a large number of viral diseases are always lacking in effective therapeutic drugs. The pathogenesis of some diseases is unclear. The abuse of a large number of broad spectrum antibiotics creates a strong selective pressure, which causes many strains to mutate, resulting in the development of drug resistance, and new threats to human health.
Microorganisms are in various states, and some are putrefactive, i.e., cause undesirable changes in food odor and texture. The microorganism can cause diseases and can cause mildewing and rotting of foods, cloth, leather and the like, but the microorganism also has a beneficial side. Penicillin was originally discovered from penicillium to inhibit the growth of other bacteria, and this was an epoch-making discovery for the medical community. Subsequently, a large number of antibiotics were selected from metabolites of actinomycetes and the like. The use of antibiotics saves countless lives in world war ii. Some microorganisms are widely used in industrial fermentation for producing ethanol, food, various enzyme preparations and the like; a part of microorganisms can degrade plastics, treat wastewater and waste gas and the like, and the potential of renewable resources is very great, so the microorganisms are called environment-friendly microorganisms; there are also some microorganisms that can survive in extreme environments, such as: in the environment where ordinary living bodies cannot survive, such as high temperature, low temperature, high salt, high alkali, high radiation, etc., some microorganisms still exist.
There are many existing methods for detecting microorganisms, for example, a volume measurement method in which the number and the type of microorganisms are determined by measuring the amount of hyphae contained in a certain volume of culture solution to reflect the growth of the microorganisms; weighing dry weight method to test the amount of microorganism in a certain weight by measuring the weight proportion of the microorganism in a certain weight by centrifugation or filtration; the hypha length measuring method measures the growth condition of microorganisms by measuring the growth length of hypha in a certain time, so as to determine the number and the type of the microorganisms; the liquid dilution method is a method in which microorganisms of a certain concentration are diluted and then cultured, thereby measuring the number of microorganisms.
However, the microbial dilution process is complicated, and generally requires many times of dilution, which is time-consuming, and the microbial culture requires much time for waiting for the microbial community to grow, so that the microbial detection process is complicated, the time required for the test is long, the efficiency is low, and the labor cost is high. Since the microorganism itself has a fast change speed and a long detection period, the actually detected result may not be consistent with the situation in the real test space. Meanwhile, sampling in a severe environment has a large safety risk. In order to solve the above technical problems, embodiments of the present invention provide a system and a method for detecting microorganisms.
The present invention is applied to a case where sterilization is performed by measuring the number and types of microorganisms in a space where sterilization is required, such as an operating room or a classroom in a hospital.
The following describes the technical solutions of the present invention and how to solve the above technical problems with specific embodiments. The following several specific embodiments may be combined with each other, and details of the same or similar concepts or processes may not be repeated in some embodiments. Embodiments of the present invention will be described below with reference to the accompanying drawings.
Fig. 1 is a schematic structural diagram of a microorganism detection system provided by the present invention, and as shown in fig. 1, the system provided by this embodiment includes: a processing assembly 1, a position detection assembly 2, a robotic arm 3, an imaging assembly 4 and a transport assembly 5.
Wherein, the processing assembly 1 is respectively connected with the position detection assembly 2, the mechanical arm 3 and the imaging assembly 4.
And the position detection assembly 2 is used for measuring the spatial position information of the space to be detected.
The processing component 1 is used for determining the position of a sampling point according to the spatial position information and controlling the mechanical arm 3 to move according to the position of the sampling point so as to obtain a sample on the sampling point;
a transport assembly 5 for transporting the sample to the imaging assembly 4;
the imaging component 4 is used for shooting the amplified sample;
the processing component 1 is also used for determining the number and the types of the microorganisms according to the images obtained by the imaging component 4.
A processing assembly 1 for controlling the overall operation of the microbiological detection system, such as the operations associated with determining sampling points, controlling the robotic arm 3, determining microbial numbers and species. The processing assembly 1 may comprise one or more processors to execute instructions. Furthermore, the processing assembly 1 may comprise one or more modules facilitating interaction between the processing assembly 1 and other components in the microbiological detection system.
And the position detection assembly 2 is used for measuring the spatial position information of the space to be detected. Wherein, wait to detect the space and include: a certain space or a certain object plane to be measured, for example, the space to be detected may be a certain operating room or a certain operating table plane. The spatial position information may be a three-dimensional point cloud distribution map of the space to be detected or the object to be detected. After the position detection component 2 measures the spatial position information of the space to be detected, the processor can determine the position of the sampling point according to the spatial position information of the space to be detected.
The mechanical arm 3 is an automatic mechanical device, and the mechanical arm 3 can rotate, stretch out and draw back or go up and down, can be after receiving the sampling instruction that processing component 1 sent, accurately fix a position to a certain sampling point on three-dimensional (or two-dimensional) space and take a sample, wherein, sampling instruction includes sampling point position information.
The imaging assembly 4 is used for magnifying and shooting the sample to obtain the image of the microorganism, and the imaging assembly 4 includes but is not limited to: the zoom lens comprises a lens, a focusing module and a shooting module, wherein the lens can be a high power magnifying lens or a microscope lens and can magnify a sample, for example, the magnification of the microscope lens on the sample can be 10 times, 20 times, 50 times, 100 times, 200 times, 500 times, 1000 times or 2000 times and the like. The focusing module can realize automatic focusing on the sample, so that the shot picture is clear and can be used for identifying the shape and the type of microorganisms. The shooting module is used for shooting the sample imaged through the lens. The processor may control the imaging assembly 4 to take a photograph.
A transport assembly 5 for transporting the sample to the imaging assembly 4. The transmission assembly 5 may be controlled by the processor to start, stop and travel speed.
In practical application, when the number and the type of microorganisms in an object or a space need to be measured, the microorganism detection system can determine the spatial position information of the space to be detected in a step measurement mode, the microorganism detection system uses the position detection component 2 to measure the spatial position information of the space to be detected in the moving process, for example, the distribution condition of the microorganisms in an operating room of a hospital needs to be measured, and the microorganism detection system measures the position information of the ground, the operating table surface, the equipment table surface, the wall surface and the like of the operating room to be detected through the position detection component 2.
The processing assembly 1 determines the position of the sampling point where the microorganism needs to be sampled according to the spatial position information, wherein the sampling point may be one or more, and the invention is not limited thereto.
In a possible implementation manner, a position condition of the sampling point to be obtained may be set, and the processing component 1 determines the position of the sampling point according to the spatial position information. For example, in an operating room of a hospital, a sampling point is set as the center point position of each surface, and the processing assembly 1 in the microorganism detection system determines the coordinates of the sampling point as the coordinates of the center point of the ground, the coordinates of the center point of the operating bed surface, the coordinates of the center point of the equipment table surface and the coordinates of the center point of the wall surface according to the spatial position information.
In another possible implementation manner, the number of sampling points and the positions of the sampling points may be determined according to the spatial position information and the sampling density. The sampling density is the number of sampling points in unit area, and the sampling densities at different positions in the space to be measured can be the same or different. The area to be sampled in the space to be detected can be determined according to the spatial position information, so that the number of sampling points and the positions of the sampling points are determined according to the sampling density, for example, the determination of the spatial position information of the operating room to be detected is as follows: the ground area of the operating room is 20 square meters, the table surface area of the operating table is 2.5 square meters, the table surface of the equipment is 2.5 square meters, and the detection area of the wall surface is 20 square meters. The sampling density is set to be 0.1 square meter, one test point is taken, the processing component 1 in the microorganism detection system calculates the number of the sampling points to be 450 according to the spatial position information and the sampling density of the operating room to be detected, and the position coordinates of each sampling point are determined.
The processing assembly 1 controls the mechanical arm 3 to move according to the position of each sampling point so as to obtain the sample on the sampling point. Wherein, the microorganism detecting system can wholly move to take a sample, also can wholly fix in a certain position, takes a sample by the motion of the processing assembly 1 control arm 3. For example, after determining the position of a sampling point in an operating room to be tested, the microorganism detection system is moved to the corresponding detection position, and the processing assembly 1 controls the mechanical arm 3 to move to obtain a sample on the sampling point.
The sample on the acquired sampling point is transferred to the imaging assembly 4 by using the transfer assembly 5, so that the imaging assembly 4 can shoot the sample. The imaging component 4 is used for magnifying the sample and shooting the sample to obtain an image of the microorganism in the sample, wherein in order to determine the quantity of the microorganism, the image of the microorganism in the sample needs to be capable of identifying the microorganism individual, and the quantity of the microorganism in the microorganism sample is determined through the lens magnification and the quantity of the microorganism individual identified in the image. In order to determine the type of microorganism, the image of the microorganism in the sample needs to clearly show the individual morphology of the microorganism. The image of the microorganism in the sample may be one or a plurality of images, the imaging component 4 focuses from low magnification to high magnification until the shape of the individual microorganism can be distinguished, at this time, an image can be obtained, the processing component 1 uses an image recognition technology to identify the number of the microorganism in the image and determine the number of the microorganism in the sample according to the magnification, and further, the processing component 1 compares the image with the prestored images of different types of microorganisms, thereby determining the type of the microorganism. If the image can identify the type of the microorganism, the sample may not be photographed any more, if the processing component 1 does not identify the type of the microorganism at this time, the image may be continuously photographed with a higher magnification than that used in photographing the image, and then compared with the prestored images of different types of microorganisms until the type of the microorganism is identified, or if the lens is adjusted to the preset magnification, the photographed image still cannot identify the type of the microorganism, and the type of the microorganism is not identified any more, and at this time, the content of the microorganism in the measured sample may be very low or no microorganism may exist, and therefore the type of the microorganism cannot be identified. For example, the imaging assembly 4 automatically focuses and sequentially photographs the sample. When the magnification of the lens is 200 times, an image of the microorganisms in a sample is shot, the number of the microorganisms on the image is calculated to be 2 by the processing component 1, and the number of the microorganisms at the sampling point is calculated to be 200/square centimeter according to the area below the lens to be 1 square millimeter. And continuously shooting an image of a microorganism when the lens magnification is 2000 times, and comparing the image with pre-stored images of different types of microorganisms to determine that the microorganism in the sample is staphylococcus albus. The type of the microorganism at the sampling point was thus determined to be Staphylococcus albus, and the number of Staphylococcus albus was 200/cm.
In this way, the kind and number of microorganisms at each sampling point were determined in turn.
Alternatively, the number and/or type of microorganisms may be plotted by data on the type and number of microorganisms at each sampling point. Illustratively, fig. 2A is a schematic diagram of the number distribution of a microorganism, fig. 2B is a schematic diagram of the number and type distribution of microorganisms, as shown in fig. 2A and 2B, X and Y respectively represent the position coordinates of a certain plane in space, the Z axis perpendicular to the X, Y axis represents the number of microorganisms, A, B, C, D, E, F represents the types of different microorganisms, the points in the diagrams represent the number of microorganisms at sampling points of the corresponding position coordinates in space, and any one of a-F marked on the points represents the type of microorganism. The number and/or the distribution map of the types of the microorganisms can be drawn through the determined types and the number of the microorganisms of each sampling point, so that the types and the number distribution of the microorganisms in the space to be detected can be displayed more visually.
In this embodiment, the microorganism detection system comprises: the device comprises a processing assembly, a position detection assembly, a mechanical arm, an imaging assembly and a transmission assembly. Wherein, the processing assembly is respectively connected with the position detection assembly, the mechanical arm and the imaging assembly. The position detection subassembly measures the spatial position information who waits to detect the space, and the processing module is according to spatial position information, confirms the position of sampling point, according to the position control arm motion of sampling point in order to obtain the sample on the sampling point, and the transmission subassembly transmits the sample to the formation of image subassembly, and the formation of image subassembly is enlargied the back and is shot the sample, and the processing module is according to the image that the formation of image subassembly was shot and is obtained, confirms the quantity and the kind of microorganism. The determination of the number and the types of the microorganisms in the space to be detected can be automatically completed through the microorganism detection system, the manual intervention is not needed in the determination process, the labor cost is saved, the detection can be directly performed after the sampling in the determination process, the detection process is short in time consumption, and the microorganism detection efficiency is improved.
Fig. 3 is a schematic structural diagram of another microorganism detection system provided by the present invention, and fig. 3 is a schematic structural diagram of a transmission assembly 5, based on the embodiment shown in fig. 1, as shown in fig. 3, including: a conveyor belt 51 and a power unit for controlling the movement of the conveyor belt 51.
A power unit may be provided below the conveyor belt 51 for controlling the movement of the conveyor belt 51, the power unit may be connected to the processing assembly 1, and the processing assembly 1 controls the start, stop and speed of the power unit.
The conveyor belt 51 has a closed shape and may have a circular or oval shape, but the present invention is not limited thereto. The conveying belt 51 is used for conveying the sample to the position below the lens of the imaging component 4 for shooting under the control of the power unit after the mechanical arm 3 acquires the sample.
Optionally, one or more stages 52 for placing the sample are further disposed on the conveyor belt 51. The carrying platform is a flat-surface object block for placing a microorganism sample, and can be a culture dish, a glass sheet, a metal sheet or a quartz sheet, and the like, the invention is not limited to the above, the carrying platform can be rectangular, square, circular or other closed shapes, and the microorganism sample can be placed on the carrying platform, and the invention is not limited to the above. The area of the stage may be any value of 1cm to 100 cm, for example, the stage may be 1cm × 1cm, or 10cm × 10 cm.
Optionally, the carrier carries a volume of sampling fluid for lysing microorganisms in the sample sampled by the robotic arm 3.
Alternatively, the power unit may be an electric motor.
Optionally, the sampling end 31 of the robotic arm 3 is detachably provided with a swab for sampling.
Wherein, the swab is a material with absorption capacity, and in the sampling process of the sampling point, the mechanical arm 3 uses the swab to repeatedly wipe the sampling point for N times, wherein N is an integer which is more than or equal to 2 and less than or equal to 20. Then the mechanical arm 3 puts the swab into the sampling liquid, so that the microorganism is dissolved in the sampling liquid, and the sampling liquid is uniformly distributed on the carrier.
Optionally, the mechanical arm 3 may contain a plurality of swabs therein, and the swabs are automatically ejected during sampling.
Alternatively, the swab may be a cotton swab.
For example, carry out the microorganism detection in the operating room of hospital, microorganism detecting system moves to the position of corresponding sampling point in proper order automatically, the cotton swab is popped out to the sampling end 31 of arm 3, control cotton swab is 5 times repeatedly and is cleaned the sampling point, then put into the sample solution of microscope carrier with the cotton swab, the microscope carrier is 5 square centimeter's square culture dish, it has 5 ml's sample solution to fill on it, repeatedly dip in and wipe 5 times, 2 rings of anticlockwise stirring simultaneously, make the microorganism dissolve in sample solution, and sample solution evenly distributed is on the microscope carrier. The stage is placed on a conveyor belt 51 and is transported to a position below the high power microscope by a 3cm step by a transport motor.
This embodiment, through the transmission band and be used for controlling the power pack of transmission band motion to take a sample to the sample of sampling point to accomplish the transmission of sample automatically, transmit the sample to the camera lens below and shoot, thereby improved microbiological detection's efficiency.
Optionally, on the basis of the foregoing embodiment, in the microorganism detection system provided in this embodiment, the position detection assembly 2 includes a first position probe and a second position probe, the first position probe is disposed at the bottom of the position detection assembly 2 body, and the second position probe is disposed on the robot arm 3;
the first position probe is used for measuring horizontal position information in the space to be detected;
and the second position probe is used for measuring height information in the space to be detected.
At a certain position, the first position probe measures the current horizontal position information in the space to be detected, and the height information in the space to be detected measured by the second position probe is combined, so that the position information coordinate of the position is obtained, the microorganism detection platform continues to move, and the space position information is measured while moving, so that the space position information of the space to be detected is determined.
Optionally, the microorganism detection system further comprises a communication component, the communication component is connected with the processing component 1, and the communication component is used for realizing data interaction between the processing component 1 and other devices. The processing component 1 can transmit data of the type and amount of microorganisms to other devices through the communication component, and the other devices can map the type and amount of microorganisms according to the type and amount of microorganisms.
An application scenario of the microorganism detection system according to the embodiment of the present invention is described below by taking the detection of the number and the type of microorganisms in a compartment of a high-speed rail as an example.
A position detection assembly in the microorganism detection system measures a high-speed rail carriage to be detected to obtain spatial position information of the high-speed rail carriage to be detected, the position detection assembly sends the spatial position information of the high-speed rail carriage to be detected to a processing assembly, and the processing assembly calculates the number of test points and corresponding test positions. The spatial position information of the high-speed rail carriage comprises information of positions of the ground, the seat surface, the seat bottom, the wall surface and the like.
The sampling density was set in the microbiological testing system, where one sampling point was taken for a ground and seat bottom sampling density of 0.1 square meters and five sampling points were taken for a seat surface sampling density of 0.1 square meters. And the processing component calculates the number of sampling points and the positions of the sampling points required in the carriage of the high-speed rail according to the spatial position information and the sampling density.
The microorganism detection system moves to corresponding positions in a high-speed rail car in sequence, the mechanical arm automatically samples, and a sampling end 31 of the mechanical arm repeatedly wipes sampling points by using a cotton swab for 10 times. The stage was a square petri dish with an area of 10cm square, and 5ml of the sample solution was filled on the stage. The sample liquid is put into to cotton swab, repeatedly dips in and wipes 5 times, and anticlockwise stirring 3 rings simultaneously makes the microorganism dissolve in sample liquid, and sample liquid evenly distributed is on the microscope carrier. The carrying platform is placed on the conveying belt and moves along with the conveying belt, and the carrying platform is conveyed to the position below the lens of the imaging assembly by a conveying motor in a stepping mode according to 5 cm.
The imaging assembly automatically focuses and sequentially shoots the microorganism samples on the carrying platform. A microorganism distribution image is shot under a low-power lens with the magnification of 200 times, the number of microorganisms on the identification image of the processing component is 4, and the number of the microorganisms at the monitoring point is calculated to be 400/square centimeter according to the area under one lens to be 1 square millimeter. And shooting a microorganism morphology image under a high-power lens with the magnification of 1000 times, and comparing the image with pre-stored images of various microorganisms by the processing assembly to determine that the microorganism is salmonella enteritidis. Thus, the type of microorganism at the sampling site was determined to be Salmonella enteritidis, and the number of Salmonella enteritidis was 400 per square centimeter.
After the platform is used, the platform is transmitted to a cleaning pool by a stepping motor, the platform is sequentially washed for 10 seconds by sulfuric acid, washed for 10 seconds by alcohol and washed for 10 seconds by clear water, and the platform after the platform is cleaned can be reused.
And continuously detecting the microbial species and the number of the residual sampling points in the carriage of the high-speed rail.
The microorganism detection system can store the detected type and quantity data of the microorganisms in the high-speed rail carriage in the memory, the microorganism detection system also comprises a 5G data transmission card which can transmit the data to other equipment, such as terminal equipment or a central control computer, and the other equipment can draw a bacterial type distribution and quantity diagram of the high-speed rail carriage so as to provide a basis for the subsequent sterilization of the high-speed rail carriage.
Fig. 4 is a schematic flow chart of a microorganism detection method provided by the present invention, and as shown in fig. 4, the method provided by the present embodiment is applied to any one of the microorganism detection systems, and the method provided by the present embodiment includes:
s401, the position detection component measures spatial position information of a space to be detected and sends the spatial position information to the processing component;
s402, the processing component determines the position of a sampling point according to the spatial position information and sends a motion control signal to the mechanical arm according to the position of the sampling point;
s403, the mechanical arm moves according to the movement control signal to obtain a sample on the sampling point;
s404, the transmission component transmits the sample to the imaging component;
s405, the imaging component amplifies the sample, shoots the sample, and sends the shot image to the processing component;
s406, the processing component determines the number and the type of the microorganisms according to the image.
Optionally, the determining, by the processing component in step S402, the position of the sampling point according to the spatial position information includes:
and the processing component determines the number of the sampling points and the positions of the sampling points according to the spatial position information and the sampling density.
Optionally, on the basis of the above embodiment, the position detecting assembly includes a first position probe and a second position probe, the first position probe is disposed at the bottom of the position detecting assembly body, and the second position probe is disposed on the robot arm;
s401 includes:
s4011, measuring horizontal position information in the space to be detected by a first position probe.
And S4012, measuring height information in the space to be detected through a second position probe.
Optionally, the method provided in this embodiment further includes:
s407, the processing component receives a data transmission request sent by the terminal equipment.
And S408, sending the number and the types of the microorganisms to the terminal equipment.
The method of the above embodiment may be implemented by using the system of the above system embodiment, and the implementation principle and technical effect are similar, which are not described herein again.
The following will illustrate the principle of the method provided in this embodiment, taking the detection of microorganisms in classrooms of schools as an example.
A position detection assembly in the microorganism detection system measures classrooms to be detected to obtain spatial position information of the classrooms to be detected, the position detection assembly sends the spatial position information to be detected to a processing assembly, and the processing assembly calculates the number of test points and corresponding test positions. The spatial position information of a school classroom includes information on the ground, the seat surface, the seat bottom, the desk, and the like.
The sampling density is set in the microbiological testing system, wherein one sampling point is taken when the sampling density of the ground and the bottom of the seat is 0.1 square meter, and five sampling points are taken when the sampling density of the surface of the seat and the desk is 0.1 square meter. And the processing component calculates the number of sampling points and the positions of the sampling points required in the classrooms of the school according to the spatial position information and the sampling density.
Microorganism detecting system moves to the relevant position in proper order in the classroom of school, and the arm is automatic to be sampled, and the sampling end of arm adopts cotton swab to clean the sampling point 5 times repeatedly. The platform was a square petri dish with an area of 4 cm square, and the platform was filled with 1ml of the sampling solution. The sample liquid is put into to cotton swab, repeatedly dips in and wipes 5 times, and anticlockwise stirring 2 circles simultaneously makes the microorganism dissolve in sample liquid, and sample liquid evenly distributed is on the microscope carrier. The carrying platform is placed on the conveying belt and moves along with the conveying belt, and the carrying platform is conveyed to the position below the lens of the imaging assembly by a conveying motor according to 2cm steps.
The imaging assembly automatically focuses and sequentially shoots the microorganism samples on the carrying platform. A microorganism distribution image is shot under a low-power lens with the magnification of 200 times, the number of microorganisms on the image identified by the processing component is 1, and the number of microorganisms at the sampling point is calculated to be 100/square centimeter according to the area under one lens to be 1 square millimeter approximately. And shooting a microorganism morphology image under a high-power lens with the magnification of 2000 times, and comparing the image with pre-stored images of various microorganisms by the processing component to determine that the microorganisms are escherichia coli. Thus, the kind of the microorganism at the sampling point was determined to be Escherichia coli, and the number of Escherichia coli was 100 per square centimeter.
After the platform is used, the platform is transmitted to a cleaning pool by a stepping motor, the platform is sequentially washed for 15 seconds by sulfuric acid, washed for 15 seconds by alcohol and washed for 15 seconds by clear water, and the platform after the platform is cleaned can be reused.
And continuously detecting the types and the quantity of the microorganisms at the rest sampling points in the classrooms of the school.
The microbial detection system can store the detected type and quantity data of microbes in the school classrooms in the memory, and also comprises a 5G data transmission card which can transmit the data to other equipment, such as terminal equipment or a central control computer, wherein the other equipment can draw a bacterial type distribution and quantity diagram of the school classrooms so as to provide a basis for the sterilization of the subsequent school classrooms.
Other embodiments of the disclosure will be apparent to those skilled in the art from consideration of the specification and practice of the disclosure disclosed herein. This application is intended to cover any variations, uses, or adaptations of the disclosure following, in general, the principles of the disclosure and including such departures from the present disclosure as come within known or customary practice within the art to which the disclosure pertains. It is intended that the specification and examples be considered as exemplary only, with a true scope and spirit of the disclosure being indicated by the following claims.
It will be understood that the present disclosure is not limited to the precise arrangements described above and shown in the drawings and that various modifications and changes may be made without departing from the scope thereof. The scope of the present disclosure is limited only by the appended claims.
Claims (10)
1. A microbial detection system, comprising: the device comprises a position detection assembly, a processing assembly, a mechanical arm, an imaging assembly and a transmission assembly, wherein the processing assembly is respectively connected with the position detection assembly, the mechanical arm and the imaging assembly;
the position detection assembly is used for measuring the spatial position information of the space to be detected;
the processing component is used for determining the position of a sampling point according to the spatial position information and controlling the mechanical arm to move according to the position of the sampling point so as to obtain a sample on the sampling point;
the transport assembly for transporting the sample to the imaging assembly;
the imaging component is used for shooting the sample after being amplified;
the processing component is also used for determining the number and the type of the microorganisms according to the image shot by the imaging component.
2. The system of claim 1, wherein the transmission assembly comprises: the conveying belt and be used for controlling the power unit of conveying belt motion.
3. The system of claim 2, wherein the processing component is specifically configured to:
and determining the number of sampling points and the positions of the sampling points according to the spatial position information and the sampling density.
4. The system of claim 2, wherein one or more stages for holding samples are also placed on the conveyor belt.
5. The system of claim 1, wherein the position sensing assembly comprises a first position probe disposed at a bottom of the position sensing assembly body and a second position probe disposed on the robotic arm;
the first position probe is used for measuring horizontal position information in the space to be detected;
and the second position probe is used for measuring the height information in the space to be detected.
6. A system according to any of claims 1-5, wherein the sampling end of the robotic arm is detachably provided with a swab for sampling.
7. A microorganism detection method applied to the microorganism detection system according to any one of claims 1 to 6, comprising:
the position detection component measures spatial position information of a space to be detected and sends the spatial position information to the processing component;
the processing component determines the position of a sampling point according to the spatial position information and sends a motion control signal to the mechanical arm according to the position of the sampling point;
the mechanical arm moves according to the motion control signal to obtain a sample on the sampling point;
the transport assembly transports the sample to the imaging assembly;
the imaging component is used for amplifying the sample and then shooting the sample, and sending the shot image to the processing component;
the processing component determines the number and type of microorganisms from the image.
8. The method of claim 7, wherein the processing component determines locations of sampling points based on the spatial location information, comprising:
and the processing component determines the number of sampling points and the positions of the sampling points according to the spatial position information and the sampling density.
9. The method of claim 7 or 8, wherein the position sensing assembly comprises a first position probe disposed at a bottom of the position sensing assembly body and a second position probe disposed on the robotic arm;
the position detection assembly measures spatial position information of a space to be detected, and comprises:
measuring horizontal position information in the space to be detected through the first position probe;
and measuring the height information in the space to be detected through the second position probe.
10. The method of claim 7, further comprising:
the processing component receives a data transmission request sent by the terminal equipment;
and sending the number and the types of the microorganisms to the terminal equipment.
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