CN112080044A - Arabic gum composition and preparation method thereof - Google Patents

Arabic gum composition and preparation method thereof Download PDF

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CN112080044A
CN112080044A CN202010763757.1A CN202010763757A CN112080044A CN 112080044 A CN112080044 A CN 112080044A CN 202010763757 A CN202010763757 A CN 202010763757A CN 112080044 A CN112080044 A CN 112080044A
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gum
acacia gum
arabic
acacia
accounts
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CN112080044B (en
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张九凯
陈颖
韩建勋
胡冰
曹际娟
韩玲钰
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Chinese Academy of Inspection and Quarantine CAIQ
Dalian Minzu University
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Dalian Minzu University
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    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08LCOMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L5/00Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/10Foods or foodstuffs containing additives; Preparation or treatment thereof containing emulsifiers
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/20Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
    • A23L29/206Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin
    • A23L29/25Exudates, e.g. gum arabic, gum acacia, gum karaya or tragacanth
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08BPOLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/006Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
    • C08B37/0063Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
    • C08B37/0072Hyaluronic acid, i.e. HA or hyaluronan; Derivatives thereof, e.g. crosslinked hyaluronic acid (hylan) or hyaluronates
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08BPOLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/006Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
    • C08B37/0087Glucomannans or galactomannans; Tara or tara gum, i.e. D-mannose and D-galactose units, e.g. from Cesalpinia spinosa; Tamarind gum, i.e. D-galactose, D-glucose and D-xylose units, e.g. from Tamarindus indica; Gum Arabic, i.e. L-arabinose, L-rhamnose, D-galactose and D-glucuronic acid units, e.g. from Acacia Senegal or Acacia Seyal; Derivatives thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08LCOMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L2205/00Polymer mixtures characterised by other features
    • C08L2205/02Polymer mixtures characterised by other features containing two or more polymers of the same C08L -group
    • C08L2205/025Polymer mixtures characterised by other features containing two or more polymers of the same C08L -group containing two or more polymers of the same hierarchy C08L, and differing only in parameters such as density, comonomer content, molecular weight, structure
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08LCOMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L2205/00Polymer mixtures characterised by other features
    • C08L2205/03Polymer mixtures characterised by other features containing three or more polymers in a blend
    • C08L2205/035Polymer mixtures characterised by other features containing three or more polymers in a blend containing four or more polymers in a blend

Abstract

The invention relates to the technical field of food chemistry, and particularly relates to a Arabic gum composition and a preparation method thereof. The composition comprises acacia gum I, acacia gum II, acacia gum III and acacia gum IV, wherein the AGP content in the acacia gum I accounts for 54 percent of the total weight of the acacia gum I, the AGP content in the acacia gum II accounts for 46 percent of the total weight of the acacia gum II, the AGP content in the acacia gum III accounts for 39 percent of the total weight of the acacia gum II, and the AGP content in the acacia gum IV accounts for 34 percent of the total weight of the acacia gum IV. The preparation method comprises the steps of degrading hyaluronic acid to obtain hyaluronic acid with different molecular weights, separating acacia gum isolated phases by the hyaluronic acid with different molecular weights, and mixing the graded acacia gums. The Arabic gum composition disclosed by the invention has high AGP content and has better viscoelasticity, water retention property and emulsibility.

Description

Arabic gum composition and preparation method thereof
Technical Field
The invention relates to the technical field of food chemistry, and particularly relates to a Arabic gum composition and a preparation method thereof.
Background
Acacia gum is an amber dry powder made from a series of processes of a sticky substance generated from the branches of acacia plants, has long been used in the food industry, and is one of the polysaccharide soluble colloids which are first allowed to be used in the food industry by the U.S. food and drug administration.
In China, with the gradual expansion of the application range of the Arabic gum and the gradual maturity of the using technology, the demand of the market for high-quality Arabic gum is greatly improved. In the food industry, gum arabic is commonly used in oil-in-water emulsions, oil microcapsules, candies, desserts, chewing gums, baked goods, ice creams and beverages. The application in the oil-in-water emulsion system and the oil microcapsule field is particularly important.
The high molecular weight polysaccharides and small amounts of proteins in gum arabic are present in three major components, Arabinogalactans (AG), Arabinogalactan proteins (AGP), and Glycoproteins (GP), respectively. Different AGP levels of gum arabic have different emulsifying properties.
Disclosure of Invention
One of the main purposes of the invention is to provide a gum arabic composition. The Arabic gum composition comprises Arabic gums with different AGP contents, and the Arabic gum composition can be used as an emulsifier, a thickening agent, a coating material, an adhesive and a capsule material in the industries of food, biomedicine and the like.
It is another object of the present invention to provide a process for the preparation of the above composition. The preparation method comprises the steps of degrading hyaluronic acid to obtain hyaluronic acid with different molecular weights, separating acacia gum isolated phases by the hyaluronic acid with different molecular weights, and mixing the graded acacia gums.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention provides a gum arabic composition, which comprises gum arabic I, gum arabic II, gum arabic III and gum arabic IV, wherein the AGP content in the gum arabic I accounts for 54% of the total weight of the gum arabic I, the AGP content in the gum arabic II accounts for 46% of the total weight of the gum arabic II, the AGP content in the gum arabic III accounts for 39% of the total weight of the gum arabic II, and the AGP content in the gum arabic IV accounts for 34% of the total weight of the gum arabic IV.
Preferably, the weight ratio of acacia gum I, acacia gum II, acacia gum III and acacia gum IV in the acacia gum composition is 30-90: 1-50: 1-30: 0.1-20.
The present invention also provides a method for preparing the above gum arabic composition, comprising: the weight average molecular weights were 16.9X 10, respectively5g/mol、15.1×105g/mol、7.3×105g/mol、3.1×105g/mol hyaluronic acid is respectively mixed with Arabic gum in water under the same concentration, corresponding mixed aqueous solutions are respectively formed after fully stirring and mixing, standing is carried out for 24-48 hours until phase separation is completely stopped, and all the graded Arabic gums are mixed according to a proportion to obtain the compound Arabic gum.
Preferably, the mass ratio of hyaluronic acid in the mixed aqueous solution is 0.25%, and the mass ratio of gum arabic is 5%.
Preferably, the temperature at which the segregated phase separation of hyaluronic acid of different molecular weights from the mixed solution of gum arabic occurs is 20 to 27 ℃.
The present invention also provides the use of the above gum arabic composition, comprising: used as emulsifier, thickener, coating material, binder or encapsulating material.
Compared with the prior art, the invention has the advantages that:
the Arabic gum composition disclosed by the invention has high AGP content and has better viscoelasticity, water retention property and emulsibility. The composition is obtained by liquid-liquid phase separation, the preparation method only relates to physical change, the chemical structure of the Arabic gum is not changed, and the composition can be safely applied to industries such as food chemistry, biological medicine and the like.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate exemplary embodiments of the invention and together with the description serve to explain the invention and not to limit the invention.
FIG. 1 is a diagram showing the behavior of separating hyaluronic acid of different molecular weights from acacia gum after mixing.
FIG. 2 is a graph showing the effect of hyaluronic acid of different molecular weights on the molecular weight fractionation of gum arabic by gel permeation chromatography and multi-angle laser light scattering.
FIG. 3 is a graph showing the content of AGP components resulting from molecular weight fractionation of hyaluronic acid of different molecular weights after isolated phase separation of the corresponding gum arabic.
FIG. 4 is a graph showing the effect of the content change of each component on the molecular weight of the acacia gum-enriched phase after the isolated phase separation of hyaluronic acid and acacia gum with different molecular weights.
Detailed Description
It is to be understood that the following detailed description is exemplary and is intended to provide further explanation of the invention as claimed. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the invention. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of the stated features, steps, operations, and/or combinations thereof, unless the context clearly indicates otherwise.
In order to make the technical solutions of the present invention more clearly understood by those skilled in the art, the technical solutions of the present invention will be described in detail below with reference to specific embodiments.
Examples
1) Preparation of hyaluronic acids of different molecular weights: by using60The gamma rays generated by Co decay irradiate and degrade Hyaluronic Acid (HA) solid powder and a solution state sample (the concentration is 2% -1% w/w) at the irradiation intensity of 25kGy to obtain samples with different molecular weights, the samples perform molecular weight and basic structure characterization analysis on hyaluronic acid with different molecular weights through a gel permeation chromatograph and a multi-angle laser light scattering instrument (GPC-MALLS), and as shown in Table 1, the prepared weight average molecular weights are respectively: 15.1X 105g/mol, 7.3X 105g/mol, 3.1X 105g/mol of hyaluronic acid.
TABLE 1 basic parameters for irradiation induced degradation of hyaluronic acid with different degrees of molecular weight
Figure BDA0002613812250000041
Note: SSI: irradiating in a solid state; ASI: irradiation in liquid state
2) Mixing hyaluronic acid with different molecular weights and Arabic gum for isolated phase separation: after hyaluronic acid samples with different molecular weights are obtained through the process of 1), the hyaluronic acid samples are respectively mixed with Arabic gum according to the mixing concentration in the table 2 to respectively form corresponding mixed aqueous solutions, the mixed aqueous solutions are kept stand for 24 hours until phase separation is completely stopped, and the classified Arabic gum is mixed according to the proportion, so that isolation type phase separation behaviors with different degrees can be generated in the mixed solutions due to thermodynamic instability, as shown in the figure 1, the solutions after the isolated type phase separation of the Arabic gum and the hyaluronic acid can be divided into an upper layer and a lower layer which are respectively a hyaluronic acid enrichment phase and an Arabic gum enrichment phase, as shown in the table 2, the volume percentage of the Arabic gum enrichment phase is increased along with the reduction of the molecular weight of.
TABLE 2 mixing concentration of hyaluronic acid and gum arabic with different molecular weights and volume ratio of each phase after phase separation
Figure BDA0002613812250000042
Figure BDA0002613812250000051
3) The isolated phase separation of hyaluronic acid and Arabic gum with different molecular weights induces the regulation and control characterization of molecular weight grading: and characterizing the acacia gum enriched phase component of the hyaluronic acid and acacia gum phase separation mixed system with different molecular weights by utilizing a gel permeation chromatography and differential detector combined technology. As shown in fig. 3, the content of AGP in gum arabic I accounts for 54% of the total weight of gum arabic I, the content of AGP in gum arabic II accounts for 46% of the total weight of gum arabic II, the content of AGP in gum arabic III accounts for 39% of the total weight of gum arabic II, and the content of AGP in gum arabic IV accounts for 34% of the total weight of gum arabic IV.
4) Mixing gum arabic I, gum arabic II, gum arabic III and gum arabic IV in a ratio of 60: 30: 20: 10 and mixing to obtain the final product.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.

Claims (6)

1. The acacia gum composition comprises acacia gum I, acacia gum II, acacia gum III and acacia gum IV, wherein the AGP content in the acacia gum I accounts for 54 percent of the total weight of the acacia gum I, the AGP content in the acacia gum II accounts for 46 percent of the total weight of the acacia gum II, the AGP content in the acacia gum III accounts for 39 percent of the total weight of the acacia gum II, and the AGP content in the acacia gum IV accounts for 34 percent of the total weight of the acacia gum IV.
2. The composition of claim 1, wherein the gum arabic composition comprises gum arabic I, gum arabic II, gum arabic III and gum arabic IV in a weight ratio of 30 to 90: 1-50: 1-30: 0.1-20.
3. The method for preparing gum arabic composition according to claim 1 or 2, wherein the weight average molecular weights are 16.9 x 10, respectively5g/mol、15.1×105g/mol、7.3×105g/mol、3.1×105g/mol hyaluronic acid is respectively mixed with Arabic gum in water under the same concentration, corresponding mixed aqueous solutions are respectively formed after fully stirring and mixing, standing is carried out for 24-48 hours until phase separation is completely stopped, and all the graded Arabic gums are mixed according to a proportion to obtain the compound Arabic gum.
4. The method according to claim 3, wherein the mass ratio of hyaluronic acid to gum arabic in the aqueous mixed solution is 0.25% and 5%.
5. The method of claim 3, wherein the temperature at which the phase separation of hyaluronic acid of different molecular weights from the mixed solution of gum arabic is carried out is 20 to 27 ℃.
6. Use of gum arabic compositions according to claims 1-2 as emulsifiers, thickeners, coating materials, binders or encapsulating materials.
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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102952274A (en) * 2012-08-01 2013-03-06 湖北工业大学 Arabic gum component grading method as well as product thereby and application of product
CN102985054A (en) * 2010-06-30 2013-03-20 弗门尼舍有限公司 Solid core coacervated capsules
WO2018027014A1 (en) * 2016-08-03 2018-02-08 Qatar Foundation For Education, Science And Community Development Polymer membrane with acacia gum (gum arabic) additive
CN109012520A (en) * 2018-07-18 2018-12-18 湖北工业大学 A method of anti-solvent is limited based on gel network and prepares core-shell microcapsule

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102985054A (en) * 2010-06-30 2013-03-20 弗门尼舍有限公司 Solid core coacervated capsules
BR112012028909A2 (en) * 2010-06-30 2016-07-26 Firmenich & Cie solid core coacervated capsules
CN102952274A (en) * 2012-08-01 2013-03-06 湖北工业大学 Arabic gum component grading method as well as product thereby and application of product
WO2018027014A1 (en) * 2016-08-03 2018-02-08 Qatar Foundation For Education, Science And Community Development Polymer membrane with acacia gum (gum arabic) additive
CN109012520A (en) * 2018-07-18 2018-12-18 湖北工业大学 A method of anti-solvent is limited based on gel network and prepares core-shell microcapsule

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BING HU 等: "Effects of temperature and solvent condition on phase separation induced molecular fractionation of gum arabic/hyaluronan aqueous mixtures", 《INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES》 *
HAN, LINGYU 等: "Effect of arabinogalactan protein complex content on emulsification performance of gum arabic", 《CARBOHYDRATE POLYMERS》 *
JIN, WEIPING 等: "Molecular migration of konjac glucomannan and gum Arabic phase separation and its application in oil-water interfaces", 《FOOD HYDROCOLLOIDS》 *
MAO PENG 等: "Phase separation induced molecular fractionation of gum arabic-Sugar beet pectin systems", 《CARBOHYDRATE POLYMERS》 *
毛鹏: "阿拉伯胶与甜菜果胶混合体系的相分离行为研究及其应用", 《中国优秀硕士学位论文全文数据库工程科技I辑》 *

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