CN112075425A - Nepeta cataria volatile oil and application thereof in prevention and treatment of maize weevil and tribolium castaneum - Google Patents
Nepeta cataria volatile oil and application thereof in prevention and treatment of maize weevil and tribolium castaneum Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
Abstract
The invention discloses a Yunnan Chenopodium ambrosioides volatile oil which comprises the following compounds in percentage by weight: (+) -4-carene 36.136%, 2-carene 23.897%, o-cymene 10.571%, ascaridole epoxide 9.047%, ascaridole 4.882%, and the balance of trace compounds. Meanwhile, the invention also discloses that the volatile oil of the Yunnan Chenopodium ambrosioides can prevent and control the corn weevil and the Triplostegia castanea by fumigation, contact killing and avoidance. The using concentrations of the Yunnan Chenopodium ambrosioides volatile oil for preventing and treating the maize weevil and the Triplostegia castanea by fumigation are 1.35mg/L and 2.83mg/L respectively. The dosages of the Yunnan Chenopodium ambrosioides volatile oil for preventing and treating the elephant corn and the Triplostegia castanea by contact killing are respectively 16.8 mu g/head and 29.35 mu g/head. The Yunnan golden larchThe using concentration of the mustard volatile oil for preventing and controlling the maize weevil and the tribolium castaneum through evasion is 0.094-58.975 mug/cm respectively2And 58.975. mu.g/cm2. The volatile oil has good prevention and treatment effects on Zephyranthus maydis and Triplostegia rubescens, and can be used for prevention and treatment by fumigation, contact killing and avoidance.
Description
Technical Field
The invention relates to the application field of botanical pesticides in storage pests, in particular to application of Yunnan chenopodium ambrosioides volatile oil in prevention and treatment of zearales and tribolium castaneum.
Background
Elephant of corn (Sitophilus zeamais Motschulsky) belongs to Coleoptera (Coleoptera) weevil (Curculionidae) insects, is distributed in countries around the world, and harms stored materials such as rice, wheat, sorghum, corn, oil plants, traditional Chinese medicinal materials, grain products and the like. The pests in grain storage are not only the main reasons for reducing the quantity and the quality of stored grains, but also important media for grain mildew and pathogenic microorganism transmission, and seriously harm the physical and mental health of consumers.
Triboporium castaneum Herbst belongs to Coleoptera (Coleoptera) Bacteroideae (Tenebrionidae) insects, is also an important worldwide pest for storing grain, has miscellaneous feeding property, strong adaptability and rapid propagation, and occurs all over the world. In addition to direct eating damage to grains, flour, oil, etc., tapeworm is also transmitted, causing animal and human diseases. The worm has odor secretion from odor gland, and its secretion contains carcinogen benzoquinone, which causes the blocking of harmful substances, generates mildewy smell, and changes color.
At present, the main prevention and control method of the storage pests is to fumigate by phosphine and methyl bromide, when the phosphine is 0.4mg/m3, poisoning symptoms such as headache and the like occur to people, and the pests generate resistance after long-term use. Methyl bromide has a strong destructive effect on the atmospheric ozone layer, and is listed as a product for stopping using by the environmental planning administration of the united nations. The volatile oil as chemical information substance plays an important role in chemical ecology, and has various action modes of attracting, refusing food, avoiding, inhibiting growth and development, poisoning and the like on insects. Compared with chemical synthetic pesticides, the plant-derived pesticide has strong target specificity, high efficiency on pests, safety on non-target organisms such as human beings, livestock, natural enemies of the pests and the like, various structures of metabolites, unique mechanism and difficult generation of drug resistance when acting on a plurality of organ systems of the insects. Has the advantages of smooth degradation path in the nature, high environmental compatibility, no environmental pollution and the like, and becomes a hotspot for creating green and environment-friendly insecticides.
Chenopodium ambrosioides (Dysphania ambrosioides Linnaeus) Mosyakin & Clemants is a plant of genus Chenopodiaceae (Chenopodiaceae), an annual or perennial herb. The native tropical America is widely distributed in most areas of China. Northern is mostly born near villages and roadside, and many places have become important garden weeds. Herbs are indicated for ascariasis, ancylostomiasis and enterobiasis, and externally indicated for skin eczema. At present, the application research of the extractive of chenopodium ambrosioides in various solvents in agricultural pests is reported in documents, and the research shows that the chenopodium ambrosioides extractive has the functions of poisoning, killing, food refusal and growth inhibition on agricultural pests such as diamondback moth, cabbage caterpillar, phyllotreta striolata, aphid and the like, and has contact killing activity on storage pests such as green bean weevil, tetranychus, phaseolus vulgaris, grain weevil, large grain beetle and the like. Researches on yellow nine-membered rice wine, Wangyiping and the like find that ethanol, acetone, ethyl acetate and petroleum ether extracts of Chenopodium ambrosioides have contact killing and fumigation activities on grain beetles, tribolium castaneum and corn weevils.
The research mainly focuses on the application of the extractive of the chenopodium ambrosioides in agricultural pests, the extractive integrates alkaloids, glycosides, flavonoids, volatile oils, organic acids and other compounds, and although the extractive of the chenopodium ambrosioides can play a positive effect in the control of the agricultural pests, the research does not solve the problem that which kind of compounds in the extractive of the chenopodium ambrosioides are effective inhibiting components and whether the effective inhibiting components can improve the inhibiting effect on the agricultural pests.
Disclosure of Invention
The invention provides a volatile oil of chenopodium ambrosioides and application thereof in preventing and treating elephant corn and tribolium castaneum, aiming at the problems existing in the existing research.
The volatile oil of the Yunnan chenopodium ambrosioides provided by the invention comprises the following compounds in percentage: (+) -4-carene 36.136%, 2-carene 23.897%, o-cymene 10.571%, ascaridole epoxide 9.047%, ascaridole 4.882%, and the balance of trace compounds.
The volatile oil of the Yunnan chenopodium ambrosioides can prevent and control the elephant corn and the tribolium castaneum by fumigation, contact killing and avoidance.
The application concentrations of the Yunnan chenopodium ambrosioides volatile oil for preventing and treating the elephant corn and the tribolium castaneum by fumigation are respectively 1.35mg/L and 2.83 mg/L.
The dosages of the Yunnan chenopodium ambrosioides volatile oil for preventing and treating the elephant corn and the red-simulated sitaglipta castanea through contact killing are respectively 16.8 mu g/head and 29.35 mu g/head.
The concentration of the volatile oil of the chenopodium ambrosioides in the invention is 0.094-58.975 mug/cm through avoiding to prevent and control the corn weevil and the tribolium castaneum2And 58.975. mu.g/cm2。
The Yunnan chenopodium ambrosioides volatile oil is used for preventing and controlling grain storage pests.
The invention has the following beneficial effects: 1. the fumigating effect of the Yunnan chenopodium ambrosioides volatile oil on the elephant corn is superior to the fumigating effect of the parsley fruit, the fennel fruit, the dill fruit, the cumin fruit, the Paulinia leaf, the metaphylium leaf, the lindera root tuber, the overground part of the Suzhou camelina, the donkey root of laozoogoya and the cephalotaxus sinensis branch leaf volatile oil reported in the current research; the fumigation effect on the red-mimosa is superior to the fumigation effect of the volatile oil of artemisia rupestris leaves, cacumen biotae, chamomile, turmeric leaves, over-ground parts of filifolium ageratoides, over-ground parts of chamomile, rhizomes of purple ginger, fructus evodiae fruits, over-ground parts of shrubby ageratoides, fructus forsythiae fruits, amomum tsao-ko fruits and alpinia formosana leaves reported in the current research, so the volatile oil of the Yunnan chenopodium ambrosioides has good development potential of fumigants.
2. The contact killing effect of the Yunnan chenopodium ambrosioides volatile oil on the maize weevils is superior to the contact killing activity of the sweet orange peel, the dill fruit, the parsley fruit, the fennel fruit, the cumin fruit, the lindera root tuber, the overground part of Suzhou camelina and the donkey root-of-laeving volatile oil on the maize weevils reported in the current research; the contact killing effect of the Yunnan chenopodium ambrosioides volatile oil on the red paddy steams is better than the contact killing activity of the volatile oil of shrubby chrysanthemum overground part, rhododendron cephalospora leaf, thin leaf chrysanthemum overground part, chamomile overground part, arborvitae leaf, turmeric leaf, Sichuan chrysanthemum morifolium and forsythia fruit on the red paddy steams.
3. The effect of Yunnan Chenopodium ambrosioides on zealand is better than that of deet, and the effect of deet on Chipset is better than that of deet in later stage.
In conclusion, the volatile oil of the Yunnan chenopodium ambrosioides has excellent effects of preventing and controlling the corn weevil and the red-simulated paddy theirs by fumigating, contact killing and avoidance, and is suitable for occasions of preventing and controlling the grain storage pests.
Detailed Description
The following detailed description of specific embodiments of the present invention is provided in conjunction with examples, but it should be understood that the scope of the present invention is not limited to the specific embodiments.
Example 1: extraction and determination of Yunnan Chenopodium ambrosioides volatile oil
The overground part of chenopodium ambrosioides in the embodiment is collected from yilian county of Kunming city, Yunnan province, longitude: 103 ° 3' 43 ", latitude: 24 ° 58' 27 ", altitude: 1742m, and drying in shade for use, wherein the extract is identified as Chenopodium ambrosioides (Dysphania ambrosioides) of Chenopodium of Chenopodiaceae by Trees research room of forest institute of university of southwest forestry.
The extraction process of the Yunnan Chenopodium ambrosioides volatile oil comprises the following steps: cutting fresh Chenopodium ambrosioides samples into pieces, putting into a 1000mL round bottom flask, adding a proper amount of water, extracting for 5h by a volatile oil extractor and a steam distillation method to obtain pale yellow oily substances, freeze-drying, preparing solutions with different concentrations, and storing at-4 ℃ for later use.
The measuring program of the Yunnan Chenopodium ambrosioides volatile oil is as follows: gas chromatography conditions: GC-MS: HP7890A-5975C, quartz capillary column (HP-5MS 30m '0.25 mm' 0.25 mm); the column temperature is that the initial temperature is kept at 50 ℃ for 5min, and the temperature is programmed to be increased to 5 ℃/min to 250 ℃; the column flow rate is 1.0 mL/min; the temperature of a sample inlet is 250 ℃; the front column pressure is 100 kPa; the sample injection amount is 2.0 mL; the split ratio is 20: 1; the carrier gas was high purity helium.
Mass spectrum conditions: an ionization mode EI; electron energy 70 ev; the gas interface temperature is 280 ℃; the ion source temperature is 230 ℃; quadrupole rod temperature 150 ℃, electron multiplier voltage: 1894 v; the characterization was performed by a Wiley7n.l standard library search. And calculating the relative content of each component by an area normalization method.
After measurement, the chemical components of the Yunnan Chenopodium ambrosioides are shown in Table 1
TABLE 1 Chenopodium ambrosioides volatile oil chemical composition
GC-MS analysis revealed that 99 compounds were detected from the chenopodium ambrosioides volatile oil, and the identified compound accounted for 99.624% of the total, of which the relative contents were higher: (+) -4-carene (36.136%); 2-carene (23.897%); o-cymene (10.571%); ascaridole epoxide (9.047%); ascaridole (4.882%), etc.
Example 2: application of volatile oil of Chenopodium ambrosioides in prevention and treatment of Sitophilus zeamais and Triplostegia castanea by fumigation
(1) Test for Zephyranthus maydis and Tribotium ferrugineum
The corn weevil source to be tested is provided by a natural interesting insect plant museum at the canal of a Zhenchuan county in Nanyang city, Henan province, a constant-temperature incubator (the temperature is 27 +/-2 ℃, the relative humidity is 75 +/-5%, the illumination period L is 16h to 8h) is used for feeding wheat, and after the wheat is placed in an insect feeding room for feeding for 7D, adults are screened out; when the new generation of imagoes appear a lot for about one week, the imagoes are screened out to be used as test insects.
The triathlete castanosomate source to be tested is provided by a natural interesting insect plant museum at the canal of the Zhen county of Nanyang city, Henan province, and is cultured in an indoor artificial climate incubator (the temperature is 27 ℃ plus or minus 2 ℃, the relative humidity is 75 percent plus or minus 5 percent, and the illumination period L is 16 h: 8h) for over 3 generations of artificial breeding. Mixing whole wheat flour and yeast according to the weight ratio of 9: 1 proportion of the mixture for feeding. Healthy adults about one week after eclosion were selected as test insects.
(2) Determination of Fumigation Activity
Diluting the volatile oil of Chenopodium ambrosioides with n-hexane to obtain 5 solutions with different concentration gradients, and measuring the fumigation activity of Chenopodium ambrosioides oil on Zealand corn weevil and Triplostegian castanea. The concentration of the solution was determined by pre-testing. The fumigation concentration of the chenopodium ambrosioides volatile oil on the elephant corn is as follows: 5mg/mL, 2.5mg/mL, 1.25mg/mL, 0.625mg/mL, 0.313 mg/mL. The fumigation concentration of the tribolium castaneum is as follows: 50mg/mL, 10mg/mL, 2mg/mL, 0.4mg/mL,0.08 mg/mL. Placing 10 insects to be tested into a glass bottle with diameter of 2.5cm, height of 5.5cm and volume of 25mL, adhering filter paper with diameter of 2.0cm to the bottle cap with solid glue, and sucking 10 μ L of different concentrationsThe solution is uniformly dropped on a filter paper sheet, and after the solution is volatilized for 20s, a bottle cap is quickly screwed down and sealed to form a closed space. N-hexane was a blank control and treatment was repeated 5 times per group. After treatment, putting the treated insects in an incubator for 24h, respectively checking the death heads of all groups of test insects, calculating the mortality and correcting the mortality by using formulas (1) and (2), and calculating the median lethal concentration LC by using a sps statistical software50(mg/L)。
Mortality (%). mortality vs. total treated insects × 100 (1)
Corrected mortality (%) - (treatment-control mortality/(1-control mortality) ] × 100 (2)
(3) Fumigation activities of the volatile oil of Chenopodium ambrosioides in Zealand and Trimeresurus castanea are shown in Table 2
TABLE 2 Fumigation Activity of Chenopodium ambrosioides volatile oil on Zealand corn elephant and Tribotium ferrugineum
Note: methyl bromide was the positive control and the data were from the literature.
As can be seen from Table 2, LC of the fumigant activity of Chenopodium ambrosioides volatile oil on Zea mays Linne50The value was 1.35 mg/L. LC higher than positive control methyl bromide50The value is 0.67mg/L, but is lower than the fumigation activity LC of the volatile oil on the maize weevil reported in the current literature50The value of (c). Such as parsley (Petroselinum crispum) fruit, fennel (Foeniculum vulgare) fruit, dill (Anethum graveolens) fruit, cumin (Cuminum cyminum) fruit, Paulinia pinata leaf, Codiaea variegatum (Codiaceae) leaf, Lindera aggregata root tuber, Mosla soochowensis (Mosla soochowensis) aerial part, Equ Roxburgh (Echinops latifolia) root, Torreya grandis (Cephalotaxus chinensis) branch leaf volatile oil LC50The values are 535.8mg/L, 442.8mg/L, 229.4mg/L,
157.1mg/L, 155.64mg/L, 72.07mg/L, 23.04mg/L, 12.19mg/L, 9.98mg/L, 5.20 mg/L. The chenopodium ambrosioides volatile oil has good development potential of fumigants.
Fumigation activity LC of chenopodium ambrosioides volatile oil on tribolium castaneum50It was 2.83 mg/L. LC higher than positive control methyl bromide50The value is 1.75mg/L, which is lower than that of volatile oil LC reported in the literature50The value is obtained. At present, more reports on the fumigation activity of plant volatile oil on tribolium castaneum are available. Such as leaves of Artemisia nivea (Artemisia dubia), leaves of Platycladus orientalis (Platycaldus orientalis), leaves of California chamomilla (Ajania montanii), leaves of Curcuma Longa (Curcuma Longa), aerial parts of Ajania tenuifolia (Ajania tenuifolia), aerial parts of Chrysanthemum morifolium (Dendranthema lavandulifolium), aerial parts of Zingiber officinale (Zingiber purpureum), fruits of Evodia rutaecarpa (Evodia rutaecarpa), aerial parts of Chrysanthemum fruticosum (Ajania fruticosa), fruits of Forsythia subspina, fruits of Amomum tsaoko, fruits of Alpinia zerumbet (Alpinia zerumbet), volatile oil Fumigation activity LC50The values were 49.54mg/L, 39.25mg/L, 34.21mg/L, 19.62mg/L, 15.88mg/L, 14.40mg/L, 13.6mg/L, 11.7mg/L, 11.52mg/L, 7.68mg/L, 5.85mg/L, 5.19mg/L, respectively. From the data, the fumigation activity of the chenopodium ambrosioides volatile oil on the tribolium castaneum is obviously superior to that of other volatile oils.
Example 3: application of Yunnan chenopodium ambrosioides volatile oil in preventing and treating elephant corn and tribolium castaneum through contact killing
(1) Test for Zephyranthus maydis and Tribotium ferrugineum
The same as in example 2.
(2) Determination of contact killing Activity
The contact activity of the chenopodium ambrosioides volatile oil on the maize weevils and the tribolium castaneum is measured by a micro-drop method. The test concentration range was determined by preliminary experiments. The contact concentration of the chenopodium ambrosioides volatile oil on the elephant corn is as follows: 112.5mg/mL, 56.25mg/mL, 28.125mg/mL, 14.06mg/mL, 7.03 mg/mL. The contact concentration of the tribolium castaneum is as follows: 50mg/mL, 10mg/mL, 2mg/mL, 0.4mg/mL,0.08 mg/mL. Dripping 0.5 μ L of solution with different concentrations onto the back of chest of Zealand corn or Tripsammophila castanea by pipette, treating 10 test insects with each concentration, repeating for 5 times, transferring to glass bottle with diameter of 2.5cm and height of 5.5cm, and culturing in incubator. N-hexane is used as a negative control, and fenpropathrin is used as a positive control. After 24h, the death condition of each group of test insects is respectively checked, and the mortality and the corrected mortality are calculated by using the formulas (1) and (2). By spssStatistical software calculation of lethal intermediate LD50(μ g/head).
(3) The results of the contact killing activity of the volatile oil of chenopodium ambrosioides on zealand tribolium castaneum and tribolium castaneum are shown in table 3.
TABLE 3 contact killing Activity of Chenopodium ambrosioides volatile oil on Zealand corn elephant and Tribotium ferrugineum
Note: fenpropathrin was the positive control and the data were from the literature.
As can be seen from Table 3, the contact killing activity LD of Chenopodium ambrosioides volatile oil on Zea mays Linne50The value was 16.80. mu.g/head, lower than the positive control fenpropathrin 40.74. mu.g/head. The contact killing activity of other plant volatile oils on Zea mays is lower than that of most plant volatile oils reported in the literature, such as sweet orange (Citrus sinensis) pericarp, dill (Anethum graveolens) fruit, parsley (Petroselinum crispum) fruit, fennel (Foeniculum vulgare) fruit, cumin (Cuminum cyminum) fruit, Lindera (Lindera aggregata) root tuber, Mosla Soochowensis (Mosla soochowensis) aerial part, and donkey-hide (Echinops latifolia) root oil LD for Zea mays contact killing activity5095.63. mu.g/head, 111.3. mu.g/head, 128.2. mu.g/head, 150.0. mu.g/head, 120.4. mu.g/head, 61.65. mu.g/head, 25.45. mu.g/head and 36.40. mu.g/head, respectively. Is higher than that of Cephalotaxus sinensis (Cephalotaxus sinensis) branch and leaf volatile oil LD508.47. mu.g/head.
Contact killing activity LD of chenopodium ambrosioides volatile oil on tribolium castaneum5029.35 mu g/head, which is higher than the value of the positive control fenpropathrin by 0.60 mu g/head, does not show stronger toxicity, but shows obvious or similar contact killing activity compared with the value of the volatile oil determined by a similar method reported in the current literature. Such as aerial parts of Ajania fruticosa (Ajania fructicosa), leaves of Rhododendron cephalospora (Rhododendron capitatum), aerial parts of Ajania tenuifolia (Ajania tenuifolia), aerial parts of chamomile (Dendranthema lavandulifolium), leaves of Platycladus orientalis (Platycaldus orientalis), leaves of Curcuma Longa (Curcuma Longa), Ajania punctifolia (Ajania potanini), fruits of Forsythia subspina, fruits of Rosaceae, fruits of Rosa rugosa (Thunb), and their mixture,Touch killing activity LD of volatile oil of Amomum tsaoko fruit, Liriope muscari (Liriope muscari) and Alpinia zerumbet (Alpinia zerumbet) leaves on tribolium castaneum50The values of (a) were 105.67. mu.g/head, 104.92. mu.g/head, 52.37. mu.g/head, 49.95. mu.g/head, 48.59 g/head, 39.0. mu.g/head, 34.18. mu.g/head, 30.13. mu.g/head, 16.52. mu.g/head, 13.36. mu.g/head and 6.59. mu.g/head, respectively.
Example 4: application of chenopodium ambrosioides volatile oil in prevention and treatment of elephant corn and tribolium castaneum through evasion
(1) Test for Zephyranthus maydis and Tribotium ferrugineum
The same as in example 2.
(2) Determination of repellent Activity
The test concentration range was determined by preliminary experiments. The chenopodium ambrosioides volatile oil is diluted into 5 solutions with different concentration gradients of 12.5mg/mL, 2.5mg/mL, 0.5mg/mL, 0.1mg/mL and 0.02mg/mL by using normal hexane for standby. A circular filter paper with the diameter of 9cm is symmetrically cut apart and is symmetrically attached to the bottom of a 9cm culture dish by using solid glue. Taking 300 mu L of n-hexane solution by using a liquid transfer gun, uniformly dripping the n-hexane solution on one half of filter paper, drawing a circle on a corner by using a pencil, sucking 300 mu L of sample solution of the chenopodium ambrosioides volatile oil with different concentrations by using the liquid transfer gun, uniformly dripping the n-hexane solution on the other half of filter paper, volatilizing for 30s, respectively transferring 20 Chihuayu beetles to the center of a culture dish, sleeving silk stockings on the culture dish, wrapping the Chihua beetles by using a preservative film for external use, fastening the Chihua beetles by using rubber bands, puncturing holes on the preservative film by using a needle, and shading and culturing by. N-hexane and diethyltoluamide (DEET: N, N-diethyl-3-methylidenzamide) were used as negative and positive controls, respectively. Each concentration was repeated 5 times. Observing the distribution condition of the test insects on the filter paper when the insects are inoculated for 2h and 4h respectively, and calculating the repelling rate (PR) by using a formula (3).
Avoidance Rate (%) of 100 × (Nc-Nt/Nc + Nt) (3)
Wherein: nc is the number of test insects appearing in the n-hexane negative control area, and Nt is the number of test insects appearing in different concentrations of Chenopodium ambrosioides solution area.
(3) The results of the activity of the volatile oil of chenopodium ambrosioides in repelling zea maydis and triboliutamaridum are shown in table 4.
TABLE 4 repellency Activity of Chenopodium ambrosioides volatile oil against Zebra and Tribotium ferrugineum
Note: deet was a positive control and data were from the literature.
The experimental results of table 4 show that: the concentration of the chenopodium ambrosioides volatile oil is within the range of 0.094-58.975 mu g/cm2, and the repellent activity to zeamais is better than that of diethyltoluamide at 2 hours; the concentration is in the range of 0.094-2.359 mu g/cm2, and the repellent activity to corn elephants is better than that of diethyltoluamide at 4 h; the concentration is 11.795-58.975 mu g/cm2, and the aversion activity to the zearales is weaker than that of the diethyltoluamide. The chenopodium ambrosioides volatile oil has the repellent activity on the tribolium castaneum, the repellent activity on the tribolium castaneum is the same as that of the diethyltoluamide at the highest test concentration of 58.975 mu g/cm2 in 2 hours, and the repellent activity on the tribolium castaneum is higher than that of the diethyltoluamide at 4 hours. In conclusion, the chenopodium ambrosioides volatile oil has good repellent activity on the zea mays within 2 hours, and is equivalent to the repellent activity of deet within 4 hours. The composition shows good repellent activity to the tribolium castaneum at 2h and at a lower concentration, and the repellent activity at the highest concentration is the same as that of the diethyltoluamide. At 4h and the highest concentration, the compound shows good avoidance activity on the tribolium castaneum.
Claims (6)
1. The Yunnan chenopodium ambrosioides volatile oil is characterized by comprising the following compounds in percentage by weight: (+) -4-carene 36.136%, 2-carene 23.897%, o-cymene 10.571%, ascaridole epoxide 9.047%, ascaridole 4.882%, and the balance of trace compounds.
2. The use of the volatile oil of Chenopodium ambrosioides of claim 1 for controlling Sitophilus zeamais and Triplostegia castanea.
3. The use of the volatile oil of chenopodium ambrosioides as claimed in claim 2 for preventing and treating the elephant corn and the tribolium castaneum, wherein the volatile oil of chenopodium ambrosioides is used for preventing and treating the elephant corn and the tribolium castaneum by fumigation, contact killing and avoidance.
4. The application of the volatile oil of chenopodium ambrosioides in preventing and treating elephantopus zealand and tribolium castaneum according to claim 3, wherein the concentrations of the volatile oil of chenopodium ambrosioides in preventing and treating elephantopus zea and tribolium castaneum through fumigation are respectively 1.35mg/L and 2.83 mg/L.
5. The application of the volatile oil of the chenopodium ambrosioides in preventing and treating the elephant corn and the tribolium castaneum as claimed in claim 3, wherein the dosages of the volatile oil of the chenopodium ambrosioides in preventing and treating the elephant corn and the tribolium castaneum by contact are respectively 16.8 μ g/head and 29.35 μ g/head.
6. The application of the volatile oil of chenopodium ambrosioides in preventing and treating elephantopus zealand and tribolium castaneum according to claim 3, wherein the use concentration of the volatile oil of chenopodium ambrosioides in preventing and treating elephantopus zealand and tribolium castaneum through avoidance is 0.094-58.975 μ g/cm2And 58.975. mu.g/cm2。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010968799.9A CN112075425A (en) | 2020-09-15 | 2020-09-15 | Nepeta cataria volatile oil and application thereof in prevention and treatment of maize weevil and tribolium castaneum |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106490026A (en) * | 2016-10-31 | 2017-03-15 | 郑州思辩科技有限公司 | A kind of storage pest insecticide and preparation method thereof |
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| CN106490026A (en) * | 2016-10-31 | 2017-03-15 | 郑州思辩科技有限公司 | A kind of storage pest insecticide and preparation method thereof |
Non-Patent Citations (6)
| Title |
|---|
| LANGSI D. J.等: "Insecticidal activity of essential oils of Chenopodium ambrosioides and Cupressus sempervirens and their binary combinations on Sitophilus zeamais", 《GSC BIOLOGICAL AND PHARMACEUTICAL SCIENCES》 * |
| SHA SHA CHU等: "Composition of essential oil of Chinese Chenopodium ambrosioidesand insecticidal activity against maize weevil,Sitophilus zeamais", 《PEST MANAG SCI》 * |
| 廖颖等: "入侵植物土荆芥化学成分及其功效研究进展 ", 《安徽农业科学》 * |
| 张方梅等: "土荆芥精油对灰茶尺蠖幼虫的熏蒸活性及对其体内两种酶活力的影响 ", 《中国植保导刊》 * |
| 梁倩等: "云南野生土荆芥挥发油的化学成分及其抑菌活性的研究 ", 《时珍国医国药》 * |
| 邓寒霜等: "土荆芥挥发油成分及其活性研究进展", 《陕西农业科学》 * |
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