CN112029682A - Pit mud functional bacterial liquid, functional bacteria and preparation method thereof - Google Patents
Pit mud functional bacterial liquid, functional bacteria and preparation method thereof Download PDFInfo
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Abstract
The invention discloses pit mud functional bacterial liquid, functional bacteria and a preparation method thereof, and particularly relates to the technical field of biological fermentation. A preparation method of pit mud functional bacterial liquid comprises the following steps: diluting yellow water according to a certain proportion, adding pit mud, and carrying out anaerobic culture at 30 ℃ and pH4.0-6.5 for 15-30 d to obtain a seed solution; transferring the seed solution into diluted yellow water, blowing off oxygen by nitrogen for 20-30 min, and carrying out anaerobic culture at 30 ℃ and pH4.0-6.5 for 15-30 d; repeating the steps for 5-8 times to obtain the functional bacterial liquid containing acid-producing flora. A preparation method of pit mud functional bacteria comprises the following steps: transferring the functional bacterial liquid into an acid-producing clostridium culture medium, and carrying out anaerobic culture for 15-30 d at 30 ℃ and pH4.0-6.5; diluting and coating the fermented bacterial liquid, and separating and screening out functional bacteria in pit mud. The technical scheme of the invention solves the problem of development and utilization of functional microorganisms in pit mud, and can be used for brewing white spirit and developing and researching functional bacteria.
Description
Technical Field
The invention relates to the technical field of biological fermentation, and particularly relates to pit mud functional bacterial liquid, functional bacteria and a preparation method thereof.
Background
In a common saying, "good liquor is obtained by well cellaring" and "good liquor still needs to be aged in a cellaring pool", the cellaring mud is a special fermentation container for the strong aromatic Chinese spirits, and along with the fermentation of one round and another round, a large amount of beneficial brewing microorganisms domesticated for a long time are enriched in the cellaring mud, and the functional microorganisms provide a prerequisite condition for producing high-quality strong aromatic Chinese spirits. Ethyl caproate, ethyl butyrate, ethyl lactate and ethyl acetate are main esters in the Luzhou-flavor liquor, and precursors (acetic acid, caproic acid and butyric acid) corresponding to the esters are mostly generated by acid-producing microorganisms in pit mud. Therefore, the development of the functional microorganisms is of great significance for improving the quality of the Luzhou-flavor liquor.
In the solid-state fermentation process of the white spirit, water generated by microbial metabolism and water which is not utilized in the fermented grains seep from the fermentation materials to the bottom of the pit to form yellow water. The yellow water contains organic acid, yeast extract, soluble starch, reducing sugar, ethanol, amino acid, etc. Because the pit mud is soaked in the yellow water for a long time, microorganisms in the pit mud also carry out mass transfer and metabolism through the yellow water. Therefore, it is feasible to take yellow water as a natural culture medium to enrich acid-producing bacteria in pit mud.
Disclosure of Invention
The invention aims to provide pit mud functional bacterial liquid, functional bacteria and a preparation method thereof, so as to solve the problem of development and utilization of functional microorganisms in pit mud.
In order to achieve the purpose, the technical scheme of the invention is as follows: a preparation method of pit mud functional bacteria comprises the following steps:
s102, adding 1 part of yellow water into 5-8 parts of water, preparing pit mud in a pit pool with the pit age of 20-50 years and normal fermentation, inoculating the pit mud into the diluted yellow water according to the mass ratio of 5-10%, adjusting the pH value to 4.0-6.5, and carrying out anaerobic culture at 30 ℃ for 15-30 days to obtain a seed solution;
s104, transferring the seed solution in the S102 to the same yellow water diluent in the step S102 according to the mass ratio of 10-30%, adjusting the pH value to 4.0-6.5, blowing off oxygen by nitrogen for 20-30 min, and then carrying out anaerobic culture at 30 ℃ for 15-30 d;
and S106, repeating the step S104 for 5-8 times to obtain the functional bacterial liquid containing the acid-producing bacteria.
The working principle of the scheme is as follows: the brewing wastewater (such as yellow water) contains a large amount of lactic acid and a small amount of ethanol. In the fermentation process of the strong aromatic Chinese spirits, the pit mud is directly soaked in the yellow water, and acid-producing bacteria in the pit mud act through the yellow water. Therefore, the microorganisms in the pit mud adapt to the environment of yellow water for a long time. Through the steps of S102-S106, acid-producing microorganisms in the pit mud are domesticated and enriched continuously, so that the pit mud functional liquid capable of metabolizing lactic acid or ethanol is prepared.
Further, the pit mud functional bacterial liquid can be applied to substance conversion of white spirit brewing and brewing wastewater.
The other technical scheme of the invention is as follows: a preparation method of pit mud functional bacteria comprises the following steps:
s108, transferring the functional bacterial liquid of S106 into an acidogenic clostridium culture medium taking lactic acid or ethanol as a carbon source according to the mass ratio of 15-30%, adjusting the pH to 4.0-6.5, and carrying out anaerobic culture at 30 ℃ for 15-30 days;
s110, diluting and coating the bacterial liquid obtained after the fermentation in the S108 into a solid acid-producing clostridium culture medium, and carrying out anaerobic culture at the temperature of 30 ℃ for 7-15 days; and (3) selecting a single colony on the plate, placing the single colony in a liquid acid-producing clostridium culture medium, carrying out anaerobic culture for 7-15 days at the temperature of 30 ℃, and detecting organic acid in fermentation liquor by adopting a gas chromatography-mass spectrometer so as to screen out an acid-producing strain.
The working principle of the scheme is as follows: since yellow water is used as a natural culture medium, the nutrient content is rich but complex, and substrates and products of metabolism of functional strains are difficult to distinguish. Therefore, the functional bacterial liquid of S106 is transferred to an acid-producing clostridium culture medium which takes ethanol or lactic acid as a unique carbon source, and the product detection can not only judge the effectiveness of domestication and enrichment of acid-producing bacteria by using yellow water, but also accurately screen a target functional strain.
Further, the acid productionThe clostridium culture medium is prepared by the following method: 20-30 g lactic acid, 1.0-3.0 g yeast extract, 0.5-1.0 g (NH)4)2SO4、0.1～0.3gMgSO4·7H20 and 0.5 to 1.0gK2HPO4Adding water to a constant volume of 1L, adjusting the pH value to 4.0-6.5 by using NaOH with the concentration of 5mol/L, sterilizing at 121 ℃ for 20-30 min, cooling the culture medium to the normal temperature, and adding 8.0-12.0 g/L sterile CaCO3。
Further, the culture medium for producing clostridium acidogenum is prepared by the following method: 1.0-3.0 g yeast extract, 0.5-1.0 g (NH)4)2SO4、0.1～0.3gMgSO4·7H20 and 0.5 to 1.0gK2HPO4Adding water to a constant volume of 1L, adjusting the pH value to 4.0-6.5 by using NaOH with the concentration of 5mol/L, sterilizing at 121 ℃ for 20-30 min, cooling the sterilized culture medium to normal temperature, and adding 10-20 g/L ethanol and 8.0-12.0 g/L sterile CaCO3。
Further, 15-20 g/L agar powder is added into the clostridium acidogenum culture medium, so that a solid culture medium is prepared.
Compared with the prior art, the beneficial effect of this scheme: acid-producing bacteria in the pit mud are enriched in yellow water for multiple rounds, so that the yellow water can be converted into functional bacteria liquid with recycling value, and the acid-producing functional bacteria in the pit mud are enriched. Acid-producing bacteria in the pit mud functional bacterial liquid are separated and screened by using an acid-producing clostridium culture medium which takes lactic acid and ethanol as unique carbon sources, and the carbon source and the acid-producing type of the acid-producing bacterial strain can be determined. The method of the invention can be used for rapidly obtaining acid-producing bacteria with pit mud function and has good practical application value for the obtained flora and single bacteria.
Detailed Description
The present invention will be described in further detail below by way of specific embodiments:
example 1:
a preparation method of pit mud functional bacterial liquid comprises the following steps:
s102, adding 1 part of yellow water into 5 parts of water, preparing pit mud in a pit pool with the pit age of 20-50 years and normal fermentation, inoculating the pit mud into the diluted yellow water according to the mass percentage of 10%, adjusting the pH value to 5.5, and carrying out anaerobic culture at 30 ℃ for 15-30 days to obtain a seed solution;
s104, transferring the seed solution of the S102 to a yellow water diluent which is the same as that in the S102 according to the mass ratio of 30%, adjusting the pH value to 5.5, blowing off oxygen by nitrogen for 20-30 min, and then carrying out anaerobic culture at 30 ℃ for 15-30 d;
and S106, repeating the step of S104 for 8 times to obtain the functional bacterial liquid containing the acid-producing bacteria group.
The functional bacterial liquid is detected by a gas chromatography-mass spectrometer, and the content of organic acid in the yellow water after dilution according to parts is compared as shown in table 1:
table 1: comparing the content of organic acid in the diluted yellow water with that in the fermented yellow water
| Acetic acid (g/L) | Propionic acid (g/L) | Butyric acid (g/L) | Hexanoic acid (g/L) | Lactic acid (g/L) | |
| Diluted yellow water | 0.57 | 0.14 | 0.35 | 0.21 | 32.11 |
| Fermented yellow water | 5.68 | 0.96 | 7.63 | 4.82 | 2.76 |
As can be seen from table 1, when the pH reaches 5.5, the microbial population in the pit mud exerts a large acid-producing effect compared to the diluted yellow water. Wherein acetic acid is increased by 8.96 times, propionic acid is increased by 5.86 times, butyric acid is increased by 20.80 times, caproic acid is increased by 21.95 times, and lactic acid is decreased by 0.91 times. Therefore, the yellow water can enrich acid-producing flora in pit mud, has a good acid-producing effect, is reflected in 'increasing already and reducing milk', and can be changed into things of value from waste.
A preparation method of pit mud functional bacteria comprises the following steps:
s108, transferring the functional bacterial liquid of S106 into an acid-producing clostridium culture medium taking lactic acid or ethanol as a carbon source according to the mass percentage of 15%, adjusting the pH to 5.5, and carrying out anaerobic culture at 30 ℃ for 15-30 days;
the culture medium for producing acid clostridium is prepared by the following method: 30g lactic acid, 2.0g yeast extract, 0.5 (NH)4)2SO4、0.1g MgSO4·7H20 and 0.5gK2HPO4And adding water to a constant volume of 1L. Adjusting the pH value to 5.5 by NaOH with the concentration of 5mol/L, sterilizing for 20-30 min at 121 ℃, adding 8.0-12.0 g/L sterile CaCO after the culture medium is cooled to normal temperature3. If replacing lactic acid with ethanol, the sterilized culture medium is cooled to room temperature, and then 20g/L ethanol and 10.0g/L sterile CaCO are added3. If the solid culture medium is to be prepared, 15g/L agar powder is required to be added into the culture medium of the clostridium acidovorans.
The fermented bacterial liquid is detected by a gas chromatograph-mass spectrometer, and the content of organic acid in the clostridium acidovorans with ethanol or lactic acid as a carbon source is shown in the following table 2:
table 2: comparison of organic acid content in Clostridium acidogenum culture with ethanol or lactic acid as carbon source
As can be seen from table 2, the acid-producing bacteria group mainly have different acid-producing types in the culture medium of acid-producing clostridium using ethanol as a carbon source and lactic acid as a carbon source, and the acid-producing bacteria group is biased toward caproic acid in the culture medium using ethanol as a sole carbon source, and biased toward butyric acid in the culture medium using lactic acid as a sole carbon source. Therefore, the flora can be enriched in strains with different functions in culture mediums with different carbon sources according to the selectivity of the flora on the carbon source.
Diluting the fermented bacterial liquid obtained in the step S108, coating the diluted bacterial liquid in a solid acid-producing clostridium culture medium, and carrying out anaerobic culture at the temperature of 30 ℃ for 7-15 days; and (2) selecting a single bacterial colony on the plate, placing the single bacterial colony in a liquid acid-producing clostridium culture medium, carrying out anaerobic culture for 7-15 days at the temperature of 30 ℃, detecting the fermented bacterial liquid by a gas chromatography-mass spectrometer, and screening 8 acid-producing functional bacteria in total, wherein 5 bacteria can produce butyric acid by using lactic acid, 2 bacteria can produce propionic acid by using lactic acid, and 1 bacteria can produce butyric acid by using ethanol.
Example 2:
a preparation method of pit mud functional bacterial liquid comprises the following steps:
s102, adding 1 part of yellow water into 6 parts of water, preparing pit mud in a pit pool with the pit age of 20-50 years and normal fermentation, inoculating the pit mud into the diluted yellow water according to the mass percentage of 8%, adjusting the pH value to 4.0, and carrying out anaerobic culture at 30 ℃ for 15-30 days to obtain a seed solution;
s104, transferring the seed solution of the S102 to a yellow water diluent which is the same as that in the S102 according to the mass percentage of 10%, adjusting the pH value to 4.0, blowing off oxygen by nitrogen for 20-30 min, and then carrying out anaerobic culture at 30 ℃ for 15-30 d;
and S106, repeating the step of S104 for 5 times to obtain the functional bacterial liquid containing the acid-producing bacteria group.
The functional bacterial liquid is detected by a gas chromatography-mass spectrometer, and the content of organic acid in the yellow water after dilution according to parts is shown in the table 3:
table 3: comparing the content of organic acid in the diluted yellow water with that in the fermented yellow water
| Acetic acid (g/L) | Propionic acid (g/L) | Butyric acid (g/L) | Hexanoic acid (g/L) | Lactic acid (g/L) | |
| Diluted yellow water | 0.43 | 0.11 | 0.29 | 0.17 | 25.82 |
| Fermented yellow water | 1.59 | 0.37 | 3.25 | 2.59 | 13.28 |
As can be seen from Table 3, at a pH of 4.0, acetic acid in the fermentation broth was increased by 2.69 times, propionic acid by 2.36 times, butyric acid by 10.21 times, caproic acid by 14.23 times, and lactic acid by 0.48 times. Under the condition, the lactic acid in the yellow water is more and is not fully converted, and although the content of other organic acids is increased, the acclimatization and enrichment effects of acid-producing functional floras are not obvious.
A preparation method of pit mud functional bacteria comprises the following steps:
s108, transferring the functional bacterial liquid of S106 into an acid-producing clostridium culture medium with lactic acid or ethanol as a carbon source according to the mass ratio of 20%, adjusting the pH to 4.0, and carrying out anaerobic culture at 30 ℃ for 15-30 days;
the culture medium for producing acid clostridium is prepared by the following method: 25g lactic acid, 1.0g yeast extract, 1.0g (NH)4)2SO4、0.3g MgSO4·7H20 and 1.0gK2HPO4And adding water to a constant volume of 1L. Adjusting the pH value to 4.0 by NaOH with the concentration of 5mol/L, sterilizing for 20-30 min at 121 ℃, adding sterile CaCO with the concentration of 12.0g/L after the culture medium is cooled to normal temperature3. If replacing lactic acid with ethanol, adding 15g/L ethanol and 12.0g/L sterile CaCO after cooling the sterilized culture medium to room temperature3. If the solid culture medium is to be prepared, agar powder with the concentration of 20g/L needs to be added into the culture medium of the clostridium acidovorans.
The fermented bacterial liquid is detected by a gas chromatograph-mass spectrometer, and the content of organic acid in the acid-producing clostridium using ethanol or lactic acid as a carbon source is shown in the following table 4:
table 4: comparison of organic acid content in Clostridium acidogenum culture with ethanol or lactic acid as carbon source
Diluting the fermented bacterial liquid obtained in the step S108, coating the diluted bacterial liquid in a solid acid-producing clostridium culture medium, and carrying out anaerobic culture at the temperature of 30 ℃ for 7-15 days; and (2) selecting a single bacterial colony on the plate, placing the single bacterial colony in a liquid acid-producing clostridium culture medium, carrying out anaerobic culture for 7-15 days at the temperature of 30 ℃, detecting the fermented bacterial liquid by a gas chromatography-mass spectrometer, and screening 2 acid-producing functional bacteria in total, wherein 1 strain can produce butyric acid by using lactic acid, and 1 strain can produce propionic acid by using lactic acid.
Example 3:
a preparation method of pit mud functional bacterial liquid comprises the following steps:
s102, adding 1 part of yellow water into 8 parts of water, preparing pit mud in a pit pool with the pit age of 20-50 years and normal fermentation, inoculating the pit mud into the diluted yellow water according to the mass percentage of 5%, adjusting the pH value to 6.5, and carrying out anaerobic culture at 30 ℃ for 15-30 days to obtain a seed solution;
s104, transferring the seed solution of the S102 to a yellow water diluent which is the same as that in the S102 according to the mass percentage of 15%, adjusting the pH value to 6.5, blowing off oxygen by nitrogen for 20-30 min, and then carrying out anaerobic culture at 30 ℃ for 15-30 d;
and S106, repeating the step of S104 for 6 times to obtain the functional bacterial liquid containing the acid-producing bacteria group.
The functional bacterial liquid is detected by a gas chromatography-mass spectrometer, and the content of organic acid in the yellow water after dilution according to parts is shown in the table 5:
table 5: comparing the content of organic acid in the diluted yellow water with that in the fermented yellow water
| Acetic acid (g/L) | Propionic acid (g/L) | Butyric acid (g/L) | Hexanoic acid (g/L) | Lactic acid (g/L) | |
| Diluted yellow water | 0.34 | 0.08 | 0.22 | 0.13 | 20.10 |
| Fermented yellow water | 3.24 | 0.43 | 5.32 | 3.01 | 3.96 |
As can be seen from table 5, at a pH of 6.5, acetic acid was increased 8.53 times, propionic acid was increased 4.38 times, butyric acid was increased 23.18 times, caproic acid was increased 22.15 times, and lactic acid was decreased 0.80 times, compared to the diluted yellow water.
A preparation method of pit mud functional bacteria comprises the following steps:
s108, transferring the functional bacterial liquid of S106 into an acid-producing clostridium culture medium taking lactic acid or ethanol as a carbon source according to the mass ratio of 30%, adjusting the pH to 6.5, and carrying out anaerobic culture at the temperature of 30 ℃ for 15-30 d;
the culture medium for producing acid clostridium is prepared by the following method: 20g lactic acid, 3.0g yeast extract, 0.7g (NH)4)2SO4、0.2g MgSO4·7H20 and 0.8gK2HPO4And adding water to a constant volume of 1L. Adjusting the pH value to 6.5 by NaOH with the concentration of 5mol/L, sterilizing at 121 ℃ for 20-30 min, cooling the culture medium to normal temperature, and addingSterile CaCO with the concentration of 8.0g/L3. If the lactic acid is replaced by ethanol, the sterilized culture medium is cooled to normal temperature, and then 10g/L ethanol and 12.0g/L sterile CaCO are added3. If the solid culture medium is to be prepared, agar powder with the concentration of 18g/L needs to be added into the haloxylon acidogenic bacterium culture medium.
The fermented bacterial liquid is detected by a gas chromatograph-mass spectrometer, and the content of organic acid in the acid-producing clostridium using ethanol or lactic acid as a carbon source is shown in the following table 6:
table 6: comparison of organic acid content in Clostridium acidogenum culture with ethanol or lactic acid as carbon source
Diluting the fermented bacterial liquid obtained in the step S108, coating the diluted bacterial liquid in a solid acid-producing clostridium culture medium, and carrying out anaerobic culture at the temperature of 30 ℃ for 7-15 days; and (2) selecting a single colony on the plate to be placed in a liquid acid-producing clostridium culture medium, carrying out anaerobic culture for 7-15 d at 30 ℃, detecting the fermented bacterial liquid by a gas chromatography-mass spectrometer, and screening 8 acid-producing functional bacteria in total, wherein 4 acid-producing bacteria capable of producing butyric acid by using lactic acid, 2 acetic acid-producing bacteria and 2 butyric acid and caproic acid-producing bacteria by using ethanol.
1 strain with the highest butyric acid yield (5.47g/L) and 1 strain with the highest caproic acid yield (1.68g/L) are respectively selected from the method, the morphological identification is carried out, DNA is extracted, a 16SrRNA primer is adopted for PCR amplification, and the amplified product is sent to Shanghai biological engineering for sequencing. And performing blast on the sequencing result in an NCBI database, and selecting a result with the comparison similarity of more than 98%. Through identification and comparison, 1 Clostridium tyrobutyricum capable of producing butyric acid by using lactic acid and 1 Clostridium krusei capable of producing caproic acid by using ethanol are determined to be screened from the functional bacterial liquid and named as D-2 and J-1 respectively, and are preserved in the wine brewing biotechnology of Sichuan university of light chemical industry and the key laboratory applied to Sichuan province.
The foregoing are merely examples of the present invention and common general knowledge of known specific structures and/or features of the schemes has not been described herein in any greater detail. It should be noted that, for those skilled in the art, without departing from the structure of the present invention, several changes and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (6)
1. A preparation method of pit mud functional bacterial liquid is characterized by comprising the following steps: the method comprises the following steps:
s102, adding 1 part of yellow water into 5-8 parts of water, preparing pit mud in a pit pool with the pit age of 20-50 years and normal fermentation, inoculating the pit mud into the diluted yellow water according to the mass ratio of 5-10%, adjusting the pH value to 4.0-6.5, and carrying out anaerobic culture at 30 ℃ for 15-30 days to obtain a seed solution;
s104, transferring the seed solution in the S102 to the same yellow water diluent in the step S102 according to the mass ratio of 10-30%, adjusting the pH value to 4.0-6.5, blowing off oxygen by nitrogen for 20-30 min, and then carrying out anaerobic culture at 30 ℃ for 15-30 d;
and S106, repeating the step S104 for 5-8 times to obtain the functional bacterial liquid containing the acid-producing bacteria.
2. An application of pit mud functional bacteria liquid in white spirit brewing and substance conversion of brewing wastewater.
3. A preparation method of pit mud functional bacteria is characterized by comprising the following steps: the method comprises the following steps:
s108, transferring the functional bacterial liquid of S106 into an acidogenic clostridium culture medium taking lactic acid or ethanol as a carbon source according to the mass ratio of 15-30%, adjusting the pH to 4.0-6.5, and carrying out anaerobic culture at 30 ℃ for 15-30 days;
s110, diluting and coating the bacterial liquid obtained after the fermentation in the S108 into a solid acid-producing clostridium culture medium, and carrying out anaerobic culture at the temperature of 30 ℃ for 7-15 days; and (3) selecting a single colony on the plate, placing the single colony in a liquid acid-producing clostridium culture medium, carrying out anaerobic culture for 7-15 days at the temperature of 30 ℃, and detecting organic acid in fermentation liquor by adopting a gas chromatography-mass spectrometer so as to screen out an acid-producing strain.
4. The method for preparing pit mud functional bacteria according to claim 3, characterized in that: the culture medium for producing the clostridium acidovorans is prepared by the following method: 20-30 g lactic acid, 1.0-3.0 g yeast extract, 0.5-1.0 g (NH)4)2SO4、0.1～0.3g MgSO4·7H20 and 0.5 to 1.0g K2HPO4Adding water to a constant volume of 1L, adjusting the pH value to 4.0-6.5 by using NaOH with the concentration of 5mol/L, sterilizing at 121 ℃ for 20-30 min, cooling the culture medium to the normal temperature, and adding 8.0-12.0 g/L sterile CaCO3。
5. The method for preparing pit mud functional bacteria according to claim 3, characterized in that: the culture medium for producing the clostridium acidovorans is prepared by the following method: 1.0-3.0 g yeast extract, 0.5-1.0 g (NH)4)2SO4、0.1～0.3g MgSO4·7H20 and 0.5 to 1.0g K2HPO4Adding water to a constant volume of 1L, adjusting the pH value to 4.0-6.5 by using NaOH with the concentration of 5mol/L, sterilizing at 121 ℃ for 20-30 min, cooling the sterilized culture medium to normal temperature, and adding 10-20 g/L ethanol and 8.0-12.0 g/L sterile CaCO3。
6. The method for preparing pit mud functional bacteria according to any one of claims 4 or 5, wherein the pit mud functional bacteria comprises the following steps: and adding 15-20 g/L agar powder into the acidogenic clostridium culture medium to prepare the solid culture medium.
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| CN113355183A (en) * | 2021-07-16 | 2021-09-07 | 四川轻化工大学 | Method for treating bottom boiler water through low-cost anaerobic fermentation |
| CN115161242A (en) * | 2022-07-26 | 2022-10-11 | 宜宾五粮液股份有限公司 | Method for directionally enriching and culturing clostridium microorganisms |
| CN115521846A (en) * | 2022-10-19 | 2022-12-27 | 江苏今世缘酒业股份有限公司 | Method for preparing high-ester seasoning wine by using yellow water, product and application |
| CN116083191A (en) * | 2022-09-07 | 2023-05-09 | 江南大学 | Method for reducing and controlling lactic acid accumulation in fermentation process of Luzhou-flavor liquor by utilizing chemotaxis of clostridium |
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| CN116083191A (en) * | 2022-09-07 | 2023-05-09 | 江南大学 | Method for reducing and controlling lactic acid accumulation in fermentation process of Luzhou-flavor liquor by utilizing chemotaxis of clostridium |
| CN116083191B (en) * | 2022-09-07 | 2024-04-30 | 江南大学 | Method for reducing and controlling lactic acid accumulation in fermentation process of Luzhou-flavor liquor by utilizing chemotaxis of clostridium |
| CN115521846A (en) * | 2022-10-19 | 2022-12-27 | 江苏今世缘酒业股份有限公司 | Method for preparing high-ester seasoning wine by using yellow water, product and application |
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