CN112029644B - Microorganism sampling device and sampling method thereof - Google Patents

Microorganism sampling device and sampling method thereof Download PDF

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Publication number
CN112029644B
CN112029644B CN202010884436.7A CN202010884436A CN112029644B CN 112029644 B CN112029644 B CN 112029644B CN 202010884436 A CN202010884436 A CN 202010884436A CN 112029644 B CN112029644 B CN 112029644B
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sampling
bag
paper
sampling bag
microorganism
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CN112029644A (en
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何东贤
于学祥
孙琪
何启盖
侯小露
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Guangxi Agricultural Vocational College
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Guangxi Agricultural Vocational College
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/14Bags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/02Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Clinical Laboratory Science (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The invention discloses a microorganism sampling device and a microorganism sampling method, and belongs to the field of microorganism sampling. The sampling device of microorganism includes the sampling bag, and the sampling bag upper end outwards turns over to form the flanging, and the flanging lower extreme is connected with the protective sheath, and the sampling bag lower extreme is located the protective sheath inboard, just is located the inboard sampling paper that is equipped with of protective sheath on the sampling bag lateral wall, is equipped with from the strip of paper used for sealing on the inside wall of flanging, is equipped with the mark bag that is used for depositing the mark card on the inside wall of sampling bag, mark bag and sampling bag fixed connection, has seted up the processing mouth that can tear in sampling bag bottom. The invention also discloses a microorganism sampling method, which ensures the aseptic operation in the collection process by adopting a microorganism sampling device, has large sampling area and large sample quantity which can be collected, is convenient for collection and treatment, and ensures no artificial pollution before, during and after collection.

Description

Microorganism sampling device and sampling method thereof
Technical Field
The invention relates to the technical field of sample collection, in particular to a microorganism sampling device and a microorganism sampling method.
Background
An infectious disease is a disease that can be transmitted to each other and is widely prevalent from person to person or person to animal, and is an infectious disease that is transmitted to another person or species via various routes. Due to variation of pathogens, animal feeding density is increased, and population and animal flows are more frequent than before, which results in infectious diseases being transmitted through air transmission, water source transmission, food transmission, contact transmission, soil transmission, vertical transmission (mother and infant transmission), body fluid transmission, faecal opening transmission and the like by directly contacting infected individuals, body fluids and excretions of infected persons, and objects contaminated by infected persons. Some of the variant pathogens remain in the environment for a long time, and may be attached to the surfaces of soles, breeding devices, clothes, transport vehicles and other articles to spread, so that the variant pathogens infect susceptible people and susceptible animals, and once an infectious disease is outbreak, serious economic losses are caused. The quarantine and monitoring of infectious diseases are of vital importance, and the sample collection is one of the important links of quarantine and detection of infectious diseases, and the accuracy and the detection efficiency of infectious disease detection are related. The collection and detection of samples in the environment are important to ensuring biological safety and controlling the transmission of infectious diseases, but the collection of pathogens in the environment is often easily ignored by people, and the sampling devices suitable for collecting environmental samples are few at present, and the sampling devices and the sampling methods suitable for collecting the pathogens in the environment are not available. Some first-line infectious disease workers can adopt a cotton swab to collect environmental samples, but the contact surface of the cotton swab is small, the collected environmental samples are small in quantity, and the post-treatment is troublesome, so that the detection work and the detection result are influenced.
Disclosure of Invention
The invention aims to provide a microorganism sampling device and a microorganism sampling method, which solve the problems that a sample cannot be collected aseptically in a large area and the sample is inconvenient to store and process. The sampling device for the microorganisms has the advantages of simple structure and easy operation, and by adopting the sampling method, the aseptic operation in the collection process is ensured, the sampling area is large, the quantity of the collected samples is large, the collection and the treatment are convenient, and the artificial pollution is not caused before, during and after the collection. The technical scheme adopted by the invention is as follows:
according to one aspect of the invention, a sampling device for microorganisms is provided, the sampling device for microorganisms comprises a sampling bag, the upper end of the sampling bag is outwards turned to form an outward turned edge, the lower end of the outward turned edge is connected with a protective sleeve, the lower end of the sampling bag is positioned on the inner side of the protective sleeve, sampling paper is arranged on the outer side wall of the sampling bag and positioned on the inner side of the protective sleeve, a self-sealing strip is arranged on the inner side wall of the outward turned edge, a marking bag for storing a marking card is arranged on the inner side wall of the sampling bag, the marking bag is fixedly connected with the sampling bag, and a tearable processing opening is formed in the bottom of the sampling bag.
Preferably, the flange is in tearable connection with the protective sheath.
Preferably, the distance between the self-sealing strip and the junction of the flanging and the protective sleeve is 1-2cm.
Preferably, the sampling paper is electrostatic dust collection paper.
Preferably, the sampling bag is made of transparent PE material.
Preferably, a sampling method of a sampling device for microorganisms includes the steps of:
(1) Firstly, stretching a hand into a sampling bag, tearing off a protective sleeve from the flanging to expose sampling paper, and keeping the flanging to be everted;
(2) Dripping buffer solution or sterilized normal saline on the sampling paper to enable the sampling paper to be in a wet state;
(3) The hand drives the sampling paper to wipe the surface of the sample object, and the microorganism adhered to the surface of the object is collected;
(4) After the sample is collected, the sampling bag is grasped by hands, the flanging is stretched, the sampling bag is turned outwards from inside to outside, the sampling paper and the self-sealing strips are positioned at the inner side of the sampling bag, the self-sealing strips at the two sides of the sampling bag are attached, the marking card is marked, and the marking card is placed into the marking bag after the name and the number of the sample are written;
(5) And (3) dripping proper buffer solution or normal saline into the sampling bag, extruding the sampling bag to enable the flushing solution or normal saline to be fully contacted with the sampling paper, extruding the sampling bag again to enable the mixed sample solution to be gathered at the treatment opening, tearing the sample treatment opening, and collecting the sample solution by using a micro centrifuge tube so as to detect the sample solution.
The technical scheme adopted by the invention has the following remarkable effects:
the microorganism sampling device has the advantages of simple structure, easy operation, large area of articles contacted with the sample in the process of collection, and large quantity of collected samples, and has double functions of convenient collection and treatment; the design of the outward flange is adopted, the outward flange is connected with the protective sleeve, the protective sleeve can be torn off before use, the sampling paper can be exposed for sampling, the sampling bag can be turned from inside to outside after the sampling is completed, the sampling paper and the self-sealing strip are positioned at the inner side of the sampling bag, and the self-sealing strip is attached, so that artificial pollution is not caused before, during and after the sampling; the sampling paper is electrostatic dust collection paper, and is provided with static electricity, and dust and pathogenic microorganisms on the surface can be adsorbed by wiping the surface of an article in the process of sampling; in addition, the design of the treatment port can be torn after the sampling and treatment are finished, and the sample liquid can be collected by the micro centrifuge tube for subsequent detection work.
Drawings
FIG. 1 is a schematic diagram of a device for sampling microorganisms prior to sampling according to the present invention;
FIG. 2 is a schematic diagram of a microorganism sampling apparatus at the time of sampling according to the present invention;
FIG. 3 is a schematic structural view of a device for sampling microorganisms according to the present invention.
1-sampling bag, 2-flanging, 3-protective sleeve, 4-sampling paper, 5-self-sealing strip, 6-marking bag, 7-treatment port and 8-marking card.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be further described in detail below by referring to the accompanying drawings and by illustrating preferred embodiments. It should be noted, however, that many of the details set forth in the description are merely provided to provide a thorough understanding of one or more aspects of the invention, and that these aspects of the invention may be practiced without these specific details.
As shown in fig. 1 to 3, according to the sampling device and the sampling method of the microorganism of the present invention, the sampling device of the microorganism comprises a sampling bag 1, the upper end of the sampling bag 1 is turned outwards to form a turned-out edge 2, the lower end of the turned-out edge 2 is connected with a protective sleeve 3, and the turned-out edge 2 is connected with the protective sleeve 3 in a tearing way. The lower extreme of sampling bag 1 is located protective sheath 3 inboard, and on sampling bag 1 lateral wall and be located protective sheath 3 inboard and be equipped with sampling paper 4, sampling paper 4 is electrostatic precipitator paper. Before sampling, the protective sleeve 3 protects the sampling paper from being polluted, and when the sampling is prepared, the protective sleeve 3 is torn off from the flanging to expose the sampling paper 4, so that the article can be sampled through the sampling paper 4. The sampling paper 4 is electrostatic dust collection paper, and is itself charged with static electricity, and dust and pathogenic microorganisms on the surface can be adsorbed by wiping the surface of the object during the sampling process. The inner side wall of the flanging 2 is provided with a self-sealing strip 5, and the distance from the self-sealing strip 5 to the joint of the flanging 2 and the protective sleeve 3 is 1cm. The inside wall of sampling bag 1 is equipped with the mark bag 6 that is used for depositing mark card 8, mark bag 6 and sampling bag 1 fixed connection, has offered the processing mouth 7 that can tear in sampling bag 1 bottom.
Wherein the sampling bag 1 is made of transparent PE material.
When the invention is used, firstly, a hand is stretched into the sampling bag 1, the protective sleeve 3 is torn off from the flanging, the sampling paper 4 is exposed, and the flanging 2 is kept to be everted; dripping buffer solution or sterilized normal saline on the sampling paper 4 to enable the sampling paper 4 to be in a wet state; the sampling paper 4 is driven by hands to wipe the surface of a sample object, and microorganisms adhered to the surface of the object are collected; after the sample collection is completed, the sampling bag is grasped by hands, the flanging 2 is stretched, the sampling bag 1 is turned from inside to outside, the sampling paper 4 and the self-sealing strip 5 are positioned at the inner side of the sampling bag 1, the self-sealing strips 5 at the two sides of the sampling bag 1 are attached, and the sample name and the number are written at the position of the marking card 8 and then are placed into the marking bag 6; the proper buffer solution or normal saline is dripped into the sampling bag 1, the sampling bag 1 is extruded, so that the flushing solution or the normal saline is fully contacted with the sampling paper 4, the sampling bag 1 is extruded again, the mixed sample solution is gathered at the treatment opening 7, the sample treatment opening is torn, and the sample solution can be collected by a micro centrifuge tube so as to be detected.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.

Claims (4)

1. A sampling device for microorganisms, characterized in that: the sampling bag comprises a sampling bag, wherein the upper end of the sampling bag is outwards turned to form an outward turned edge, the lower end of the outward turned edge is connected with a protective sleeve, the lower end of the sampling bag is positioned on the inner side of the protective sleeve, sampling paper is arranged on the outer side wall of the sampling bag and positioned on the inner side of the protective sleeve, a self-sealing strip is arranged on the inner side wall of the outward turned edge, a marking bag for storing a marking card is arranged on the inner side wall of the sampling bag, the marking bag is fixedly connected with the sampling bag, and a tearable treatment opening is formed in the bottom of the sampling bag; the turnup is connected with the protective sleeve in a tearing way;
the distance between the self-sealing strip and the junction of the outward flange and the protective sleeve is 1-2cm.
2. A device for sampling a microorganism according to claim 1, wherein: the sampling paper is electrostatic dust collection paper.
3. A device for sampling a microorganism according to claim 1, wherein: the sampling bag is made of transparent PE material.
4. A sampling method of a sampling device for microorganisms according to any one of claims 1 to 3, characterized in that: the method comprises the following steps:
(1) Firstly, stretching a hand into a sampling bag, tearing off a protective sleeve from the flanging to expose sampling paper, and keeping the flanging to be everted;
(2) Dripping buffer solution or sterilized normal saline on the sampling paper to enable the sampling paper to be in a wet state;
(3) The hand drives the sampling paper to wipe the surface of the sample object, and the microorganism adhered to the surface of the object is collected;
(4) After the sample is collected, the sampling bag is grasped by hands, the flanging is stretched, the sampling bag is turned outwards from inside to outside, the sampling paper and the self-sealing strips are positioned at the inner side of the sampling bag, the self-sealing strips at the two sides of the sampling bag are attached, the marking card is marked, and the marking card is placed into the marking bag after the name and the number of the sample are written;
(5) And (3) dripping proper buffer solution or normal saline into the sampling bag, extruding the sampling bag to enable the flushing solution or normal saline to be fully contacted with the sampling paper, extruding the sampling bag again to enable the mixed sample solution to be gathered at the treatment opening, tearing the sample treatment opening, and collecting the sample solution by using a micro centrifuge tube so as to detect the sample solution.
CN202010884436.7A 2020-08-28 2020-08-28 Microorganism sampling device and sampling method thereof Active CN112029644B (en)

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CN112029644B true CN112029644B (en) 2023-11-21

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Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4262677A (en) * 1979-03-26 1981-04-21 Bader Robert F Culture sampling device and method
CN1178578A (en) * 1995-03-14 1998-04-08 霍华德·M·钱德勒 Sample collection device
CN203544627U (en) * 2013-10-25 2014-04-16 中国食品药品检定研究院食品化妆品检定所 Sterilized sampling bag
CN204556296U (en) * 2015-04-28 2015-08-12 福建农林大学 forest soil moisturizing sampling bag
CN204776544U (en) * 2015-06-29 2015-11-18 重庆北芝堂生物科技有限公司 Double -deck sampling bag
CN105087753A (en) * 2015-09-02 2015-11-25 河南农业大学 Methods for sampling and detecting microorganisms on contact surface
CN210455629U (en) * 2019-05-29 2020-05-05 广西农业职业技术学院 Integrated test tube box is cultivateed in sterilization
CN111164016A (en) * 2017-10-19 2020-05-15 嘉吉公司 Sampling apparatus and method
CN211002576U (en) * 2019-06-13 2020-07-14 李超 Novel aseptic sampling bag
CN212513766U (en) * 2020-07-06 2021-02-09 福建省国鼎检测技术有限公司 Novel food aseptic sampling bag

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3000546B1 (en) * 2012-12-28 2014-12-19 Biomerieux Sa DEVICE ADAPTED TO RECEIVE A BIOLOGICAL SAMPLE
FR3019473B1 (en) * 2014-04-04 2016-05-06 Prestodiag METHOD OF MICROBIOLOGICAL ANALYSIS OF A SAMPLE IN A SINGLE CONTAINER

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4262677A (en) * 1979-03-26 1981-04-21 Bader Robert F Culture sampling device and method
CN1178578A (en) * 1995-03-14 1998-04-08 霍华德·M·钱德勒 Sample collection device
CN203544627U (en) * 2013-10-25 2014-04-16 中国食品药品检定研究院食品化妆品检定所 Sterilized sampling bag
CN204556296U (en) * 2015-04-28 2015-08-12 福建农林大学 forest soil moisturizing sampling bag
CN204776544U (en) * 2015-06-29 2015-11-18 重庆北芝堂生物科技有限公司 Double -deck sampling bag
CN105087753A (en) * 2015-09-02 2015-11-25 河南农业大学 Methods for sampling and detecting microorganisms on contact surface
CN111164016A (en) * 2017-10-19 2020-05-15 嘉吉公司 Sampling apparatus and method
CN210455629U (en) * 2019-05-29 2020-05-05 广西农业职业技术学院 Integrated test tube box is cultivateed in sterilization
CN211002576U (en) * 2019-06-13 2020-07-14 李超 Novel aseptic sampling bag
CN212513766U (en) * 2020-07-06 2021-02-09 福建省国鼎检测技术有限公司 Novel food aseptic sampling bag

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