CN112022686A - New application of graphene in amplitude improvement and channel following conduction of microvascular vasomotor - Google Patents
New application of graphene in amplitude improvement and channel following conduction of microvascular vasomotor Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61H2201/02—Characteristics of apparatus not provided for in the preceding codes heated or cooled
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Abstract
The present disclosure relates to novel uses of graphene for microvascular vasomotor amplitude enhancement and channel conduction, including use in the manufacture of a device for enhancing microvascular vasomotor amplitude and use in the manufacture of a device for inducing microvascular vasomotor activity channel conduction. The hot application of the graphene can obviously improve the amplitude of the microvascular autonomic vasomotor activity, so that the microvascular vasomotor activity is conducted along the channels and collaterals, and the treatment of diseases is facilitated.
Description
Technical Field
The present disclosure relates to applications of graphene fever products in disease treatment, and in particular to a new use of graphene in improving microvascular vasomotor amplitude.
Background
Microvascular vasomotor activity or microvascular autonomic motion refers to rhythmic contraction and expansion motion of the microvasculature, and is the active periodic oscillation of the microvasculature (billows and the like) without depending on heart beat, respiration and nerve conduction. The frequency and the amplitude are two main characteristic indexes of the microvascular vasomotor activity, acupuncture or traditional Chinese medicine has obvious influence on the amplitude of the microvascular vasomotor activity, and the frequency of the microvascular vasomotor activity is not obviously changed generally.
After the amplitude of the microvascular autoregulation relaxation activity is increased, the sensitivity threshold of microvascular endothelial cells can be obviously increased; can obviously improve the structure and the function of the endothelial cells of the microvascular, so that the endothelial cells can better maintain the environment of the cells; can use the vasodilating substance and the vasoconstricting substance secreted by the microvascular endothelial cells as the information substance to ensure that the antagonist substances around the cells are in a high-level equilibrium state, thereby repairing the imbalance among the antagonist substances caused by the pathogenic factors.
Disclosure of Invention
The technical problem to be solved by the present disclosure is to provide a new use of graphene in improving microvascular vasomotor amplitude.
The inventor finds that the common hot compress by a hot towel and the like can only expand capillary vessels and accelerate blood circulation, but cannot improve the vasomotor activity, and by applying the graphene heating product to beagle dogs and human bodies, the inventor finds that the application of the graphene heating product on the surface of the skin can obviously improve the vasomotor activity amplitude, and the vasomotor activity amplitude can be conducted along the channels.
The present disclosure provides new uses for graphene. Specifically, the present disclosure proposes the following technical solutions:
the present disclosure provides in a first aspect the use of graphene in the manufacture of a device for increasing microvascular vasomotor amplitude.
A second aspect of the present disclosure provides use of graphene in the manufacture of a device for inducing microvascular vasomotor activity to circulate meridian conduction.
In addition, in the above-mentioned use, the device includes a graphene electrothermal membrane.
In addition, in the above-mentioned application, the graphene electric heating diaphragm includes a graphene thin film, an electrode and an insulating protection layer, the electrode is disposed on a surface of the graphene thin film, and the insulating protection layer sandwiches the graphene thin film and the electrode.
The beneficial effects of this disclosure include:
the thermal pasting method of the graphene can obviously improve the amplitude of the microvascular autonomic vasomotor activity, and the microvascular vasomotor activity caused by thermal pasting of the graphene to acupuncture points can be conducted along meridians. The effect of treating diseases can be achieved by improving the amplitude of the microvascular autonomous relaxation activity.
Compared with other methods for improving the amplitude of the microvascular autonomous relaxation activity such as acupuncture and medicine, the method for thermally pasting the graphene overcomes the inconvenience brought to patients by professionals and special tools for acupuncture therapy application, and avoids the possible drug sensitization generated by the drug therapy.
Drawings
Fig. 1 is a graph showing the change in blood flow of beagle dogs before and after graphene irradiation in example 1.
Detailed Description
The technical solutions of the present disclosure will be clearly and completely described below. Obviously, all other embodiments obtained by a person of ordinary skill in the art without making creative efforts based on the specific embodiments in the present disclosure belong to the protection scope of the present disclosure.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present disclosure.
The term "graphene" is a two-dimensional carbon nanomaterial consisting of carbon atoms in a hexagonal honeycomb lattice with sp2 hybridized orbitals.
The term "amplitude of microvascular vasomotor activity" refers to the difference between the diameter at vasodilation and the diameter at vasoconstriction, a physical quantity that describes the intensity of movement of microvascular vasomotor activity, which amplitude can vary for the same microvascular population.
The term "microcirculation perfusion" refers to the amount of blood flowing into the microvessels per unit time.
The term "meridian-following conduction" refers to a phenomenon in TCM, which is the sensory conduction phenomenon of sour, numb, distending, pain, ant-walking, etc. along the meridian-following route from the stimulated meridian point when the subject stimulates the meridian point by various methods.
The term "PU value" (Perfusion Unit) is a basic indicator of a laser doppler blood flow meter, i.e., a doppler shift value generated by flowing RBCs (red blood cells), and is a relative Unit representing the magnitude of local tissue microcirculation blood flow in a measurement depth, and the change of the PU value directly reflects the change of the tissue microcirculation blood flow.
As described above, the present inventors found that heating the skin with the graphene heating product can significantly increase the amplitude of the microvascular autonomic activity, and solve the technical problems of inconvenience or difficulty in needle application due to acupuncture or the allergic problems of drugs.
The method of the present disclosure is illustrated by the following specific examples. Reagents or equipment or procedures not described herein are routinely determinable by one of ordinary skill in the art.
In order to observe the influence of graphene heating on the amplitude of microvascular vasomotor activity, the inventor prepares graphene into a patch (the specific structure can refer to a physical therapy patch provided in patent CN 208241915U), and the graphene is respectively acted on the Mingmen acupoint and the Baihui acupoint of beagle dogs and the left and right Hegu acupoints of volunteers, and the rule of the microvascular vasomotor activity in the acupoint skin and the condition of the microvascular voluntary vasomotor motion of different acupoints of the same meridian are measured by a laser Doppler blood flow perfusion imager.
Example 1 Effect of graphene on Microvasomotor Activity amplitude, microcirculation perfusion volume and channel-following conduction in beagle dogs
1 test Material
Materials: graphene application, acupoint color developing agent and normal saline
The instrument comprises the following steps: PeriFlux System 5000 type laser Doppler blood flow instrument, Boyu BY2005S single-path DC stabilized power supply, UT39 series novel digital multimeter
2 test method
(1) Selecting the acupoint to be tested
In order to facilitate the application of the graphene patch to the acupuncture points and ensure the accuracy of detection and operation, a Mingmen acupuncture point and a waist Baihui acupuncture point are selected, the Mingmen acupuncture point and the waist Baihui acupuncture point are positioned on the back of the beagle dog, and the distance between the two acupuncture points is about 10 cm.
(2) Preparation of color developing agent for acupuncture points
Boiling 100ml distilled water, adding 9g methyl cellulose, stirring, adding 0.8g phenolphthalein reagent, stirring, adding 1ml of 15% methyl hydroxybenzoate and 2.5% ethyl hydroxybenzoate respectively, and 2ml of 10% benzalkonium bromide, and cooling.
(3) Color location of acupuncture points by using low resistance characteristic of acupuncture points
Roughly determining the positions of acupuncture points according to the anatomical parts, removing hairs on the surface, scrubbing with clear water, and drying. An acupuncture point electrode (a copper sheet with the diameter of 20mm and the thickness of 0.5mm) is lightly placed on an acupuncture point to be detected and connected with the cathode of a Boyu BY3005S single-path direct-current stabilized power supply; the reference electrode (two stainless steel sheets of 40x20mm, thickness 0.2mm) is clamped at the root of the tested dog ear with gauze padded with physiological saline, connected with the positive electrode of a power supply, and connected with a novel UT39 series digital multimeter in series in the middle, wherein the multimeter uses 2mA grade. And switching on the power supply, observing the reading of the digital multimeter, gradually increasing the current reading along with the prolonging of time, and controlling the output voltage of the power supply at any time to control the current intensity. And when the current reaches 0.3mA, timing for 60s, cutting off the power supply, and coating a color developing agent on the action part of the acupoint electrode to display the rose small dots.
(4) Detection of amplitude of microvascular vasomotor activity
The beagle dogs were kept as stable as possible during the test. Before the experiment, the laser Doppler blood flow instrument is started, the PROBE is connected, the preheating is carried out for 20min, the PROBE407 PROBE of the laser Doppler blood flow instrument is fixed at the acupoint, and the PROBE is required to be vertical to the tested part. Recording is started when the pattern motion changes tend to be stable. After recording for 5 minutes, the PROBE is removed from the acupoint, the surface temperature of the graphene application is set to be 37 ℃, the graphene application is applied to the acupoint for 20 minutes, the graphene application is taken down after 20 minutes, the PROBE407 PROBE of the laser Doppler blood flow instrument is fixed to the acupoint, and recording is carried out for 5 minutes. When the detection of the amplitude of the microvascular vasomotor activity and the microcirculation blood perfusion amount is carried out, the PROBE407 PROBE tests that the acupuncture point and the graphene application acupuncture point are the same; when the meridian-following conduction detection is carried out, the PROBE407 PROBE tests that the acupuncture points and the graphene application acupuncture points are different.
(5) Image processing and data analysis
The blood flow change curve obtained in the whole measuring process is compressed by Perimed analysis software to obtain oscillograms of the microvascular vasomotor activity changing along with time before and after the graphene application, as shown in figure 1, in the test, the amplitude is calculated by intercepting a plurality of sections of oscillograms, and each section is calculated by the difference between the maximum PU value and the minimum PU value of each section.
In the graphene application group, 5 sections of data are respectively selected from micro-vasomotor activity amplitude images of experimental beagle dogs before and after graphene irradiation to calculate the average amplitude value and amplitude variation amplitude of acupuncture point areas before and after graphene irradiation. Meanwhile, monitoring of the blood perfusion amount of acupuncture points is kept in the whole irradiation process, and the average value of the blood perfusion amount of the micro-vessels before and after graphene irradiation is counted.
Amplitude before (middle, after) graphene irradiation is (PUMax-PUMIN)/5
Amplitude variation range-average amplitude after graphene irradiation-average amplitude before graphene irradiation.
Data were processed with prism statistical software. The results are shown in tables 1, 2 and 3.
TABLE 1 microcirculation blood perfusion statistics before and after graphene application
And (4) conclusion: after the graphene is applied and pasted on the waist Baihui acupoint of a beagle dog, the microcirculation blood flow perfusion amount of the waist Baihui acupoint area of the beagle dog is improved by 1.68 times at least, 5.54 times at most and 3.02 times on average.
TABLE 2 statistics of microvascular vasomotor activity amplitude before and after graphene application
And (4) conclusion: after the graphene is applied and pasted on the waist Baihui acupoint of a beagle dog, the amplitude of the microvascular vasomotor activity of the waist Baihui acupoint of the beagle dog to be tested is improved by 2.56 times at least, 7.96 times at most and 4.85 times on average.
TABLE 3 statistics of microvascular vasomotor activity along the meridian conduction amplitude changes before and after graphene application
And (4) conclusion: after the graphene is applied and pasted on the Mingmen acupoint of a beagle dog, the amplitude of the microvascular vasomotor activity in the lumbar centesis acupoint area of the beagle dog to be tested is improved by 2.27 times at least, is improved by 3.24 times at most and is improved by 2.95 times on average.
Example 2 influence of graphene on the amplitude of microvascular autoregulatory motility, microcirculation perfusion and conduction along the channels in the acupoint region of the human body
1 test Material
Same as example 1
2 test method
(1) Selecting the acupoint to be tested
According to the frequency of use and the difficulty of operation of clinical acupuncture points of traditional Chinese medicine, a Hegu acupuncture point and a Quchi acupuncture point on the left side or the right side are specially selected, the Hegu acupuncture point and the Quchi acupuncture point are arranged on the arms and the hands of a human body, and the distance between the two acupuncture points is about 20 cm.
(2) Preparation of color developing agent for acupuncture points
Same as example 1
(3) Color location of acupuncture points by using low resistance characteristic of acupuncture points
The positions of the acupuncture points are roughly determined according to the anatomical parts, and the acupuncture points are scrubbed clean by using clean water and dried. Lightly pressing the acupoint electrode (copper sheet with diameter of 20mm and thickness of 0.5mm) at the position to be developed, and connecting with cathode of Boyu BY3005S single-way DC regulated power supply; the reference electrode (two stainless steel sheets of 40x20mm, thickness 0.2mm) is clamped on the ear drop part of the testee by gauze which is soaked with physiological saline, and is connected with the positive electrode of a power supply, and a UT39 series novel digital multimeter is connected in series in the middle, and the multimeter uses 2mA grade. And switching on the power supply, observing the reading of the digital multimeter, gradually increasing the current reading along with the prolonging of time, and controlling the output voltage of the power supply at any time to control the current intensity. And when the current reaches 0.3mA, timing for 60s, cutting off the power supply, and coating a color developing agent on the action part of the acupoint electrode to display the rose small dots.
(4) Detection of amplitude of microvascular vasomotor activity
Subjects should avoid any activity affecting the perfusion of skin blood flow prior to the test and acclimate to the environment for 20 minutes in a quiet room at 25 ℃ room temperature prior to the test. In order to avoid the interference of artifacts caused by the physical movement of the subject, the subject should be kept as stable as possible during the test. Before the test, the laser Doppler blood flow instrument is started, the PROBE is connected, the preheating is carried out for 20min, the PROBE407 PROBE of the laser Doppler blood flow instrument is fixed at the acupoint, and the PROBE is required to be vertical to the tested part. Recording is started when the pattern motion changes tend to be stable. After recording for 5 minutes, the PROBE is removed from the acupoint, the surface temperature of the graphene application is set to be 37 ℃, the graphene application is applied to the acupoint for 20 minutes, the graphene application is taken down after 20 minutes, the PROBE407 PROBE of the laser Doppler blood flow instrument is fixed to the acupoint, and recording is carried out for 5 minutes.
(5) Image processing and data analysis
And (3) compressing the blood flow change curve obtained in the whole measuring process by using Perimed analysis software to obtain oscillograms of the microvascular vasomotor activity changing along with time before and after the graphene application, wherein in the test, the amplitude is calculated by intercepting a plurality of sections of oscillograms, and each section is calculated by the difference between the maximum PU value and the minimum PU value of each section. The results are shown in tables 4, 5 and 6.
TABLE 4 statistics of microcirculation blood perfusion volume of Hegu acupoint before and after graphene application
And (4) conclusion: after the graphene is pasted on the Hegu acupoint of a subject, the microcirculation blood flow perfusion amount of the Hegu acupoint area of the subject is improved by 1.87 times at least, 5.99 times at most and 3.71 times on average.
TABLE 5 statistics of the amplitude of the microvascular vasomotor activity of the Hegu acupoint before and after application of graphene
And (4) conclusion: after the graphene is pasted on the Hegu acupoint of a subject, the amplitude of the microvascular vasomotor activity in the Hegu acupoint area of the subject is improved by 0.52 times at least, 17.6 times at most and 7.06 times on average.
TABLE 6 statistics of conduction results of the front and back channels along the applied graphene
And (4) conclusion: after the graphene is pasted on the Quchi acupoint of a subject, the amplitude of the microvascular vasomotor activity in the Hegu acupoint area of the subject is improved by 1.4 times at least, 3.71 times at most and 2.5 times on average.
Obviously, the above examples demonstrate that the acupuncture point application of graphene can significantly improve the amplitude of human body microvascular autorhythmic vasomotor activity and the perfusion amount of microcirculation blood flow, and the microvascular vasomotor activity caused by the application of graphene to a human acupuncture point can be conducted along channels.
The above description is only for the specific embodiments of the present disclosure, but the scope of the present disclosure is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present disclosure, and all the changes or substitutions should be covered within the scope of the present disclosure. Therefore, the protection scope of the present disclosure shall be subject to the protection scope of the claims.
Claims (4)
1. Use of graphene in the manufacture of a device for increasing microvascular vasomotor amplitude.
2. Use of graphene in the manufacture of a device for causing microvascular vasomotor activity to circulate meridian conduction.
3. Use according to claim 1 or 2, wherein the device comprises a graphene electro-thermal membrane.
4. The use according to claim 3, wherein the graphene electric heating diaphragm comprises a graphene thin film, an electrode and an insulating protection layer, wherein the electrode is arranged on the surface of the graphene thin film, and the insulating protection layer sandwiches the graphene thin film and the electrode.
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| PCT/CN2021/074188 WO2022021826A1 (en) | 2020-07-29 | 2021-01-28 | Novel use of graphene for micro-vasomotor amplitude enhancement and meridian and collateral conduction |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022021826A1 (en) * | 2020-07-29 | 2022-02-03 | 烯旺新材料科技股份有限公司 | Novel use of graphene for micro-vasomotor amplitude enhancement and meridian and collateral conduction |
| CN114875018A (en) * | 2022-05-11 | 2022-08-09 | 北京农学院 | Application of graphene irradiation in improving insulin activity in vitro |
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