CN112022091A - Animal behavioristics test system - Google Patents
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- CN112022091A CN112022091A CN201910483279.6A CN201910483279A CN112022091A CN 112022091 A CN112022091 A CN 112022091A CN 201910483279 A CN201910483279 A CN 201910483279A CN 112022091 A CN112022091 A CN 112022091A
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Abstract
The invention discloses an animal ethology testing system which comprises an ethology box body, a stimulation presenting module and a video monitoring module, wherein the ethology box body is configured to accommodate an experimental animal, the stimulation presenting module is configured to present stimulation towards the experimental animal, a light output end of a light pulse stimulation module extends into the ethology box body and is suitable for being connected with the head of the experimental animal, the video monitoring module comprises a visible light monitoring component and an infrared monitoring component, the visible light monitoring component is configured to monitor the behavior of the experimental animal under the condition of sufficient light, and the infrared monitoring component is configured to monitor the behavior of the experimental animal under the dark condition. The animal behavior testing system can monitor the experimental animals under the conditions of sufficient light and darkness, has good stimulation accuracy on the experimental animals, and has comprehensive functions.
Description
Technical Field
The invention relates to the technical field of life science research equipment, in particular to an animal behavioristics test system.
Background
There are various commercially mature animal behavioristics monitoring devices in the market, such as open field test, water maze, eight-arm maze, three-box social test, etc., but the behavioural system applied to instinct fear and arousal state research is still lacking. The existing animal behavioristics testing device has the following defects that firstly, the behavioristics testing device is poor in airtightness, when the device is applied to the research of mice in an anesthesia state, anesthetic gas is easy to diffuse, the personal safety of experimental researchers is influenced, and the monitoring mode of animal behaviors is single. Secondly, simple camera shooting can be performed only under the condition of sufficient light, and behavior monitoring cannot be performed in a dark environment; thirdly, most of experimental stimulation needs manual triggering and application, subjective influence is large, dependence on research personnel is also large, and meanwhile time accuracy is poor; fourthly, the function is single, and the application range of the behavior research device is limited.
Disclosure of Invention
The invention aims to provide an animal behavioristics testing system which can monitor experimental animals under the conditions of sufficient light and darkness, has good stimulation accuracy on the experimental animals and has more comprehensive functions.
In order to achieve the above purpose, the animal behavior test system of the invention adopts the following technical scheme.
An animal behavioral testing system comprising: a behavioural box configured to hold an experimental animal; the ethology box body is provided with a gas inlet, and anesthetic gas can be input into the ethology box body through the gas inlet; a stimulus presentation module disposed on the behavioral box, the stimulus presentation module configured to present a stimulus towards an experimental animal; the optical output end of the optical pulse stimulation module extends into the ethology box body and is suitable for being connected with the head of the experimental animal; the video monitoring module comprises a visible light monitoring component and an infrared monitoring component, the visible light monitoring component is configured to monitor the behavior of the experimental animal under the condition of sufficient light, and the infrared monitoring component is configured to monitor the behavior of the experimental animal under the dark condition.
In some embodiments, the behavioural case comprises: the visible light monitoring assembly is arranged corresponding to the light transmitting sheet of the main box body; the auxiliary box body is communicated with the main box body, the volume of the auxiliary box body is smaller than that of the main box body, at least one side face of the auxiliary box body is an infrared filter, and the infrared monitoring assembly corresponds to the infrared filter of the auxiliary box body.
In some specific embodiments, an opening is provided on the top wall of the main box, and the stimulation presenting module is a screen fitted at the opening.
In some optional embodiments, the auxiliary box body is a rectangular parallelepiped, and all side surfaces of the auxiliary box body are infrared filters.
In some embodiments, the animal behavioral testing system further comprises: a gas inlet tube in communication with the gas inlet, the gas inlet tube configured to input anesthetic gas into the behavioral compartment; a gas recovery pipe in communication with the ethology tank, the gas recovery pipe configured to recover gas inside the ethology tank; the gas sampling pipe is communicated with the ethology box body and configured to extract gas inside the ethology box body and output the gas to an external detection device.
In some specific embodiments, the pipe diameter of the gas recovery pipe is greater than the pipe diameter of the gas inlet pipe, and the pipe diameter of the gas inlet pipe is greater than the pipe diameter of the gas sampling pipe.
In some embodiments, the optical pulse stimulation module comprises: the laser generator is connected with the head of the experimental animal through an optical fiber; and the waveform generator is connected with the laser generator so as to adjust laser parameters emitted by the laser generator.
In some specific embodiments, the optical pulse stimulation module further comprises a suspension rod, one end of the suspension rod is connected to the inner wall of the ethology box body, the other end of the suspension rod is provided with a universal joint, and the optical fiber penetrates through the universal joint and then is connected with the head of the experimental animal.
In some optional embodiments, the ethological box body is provided with an adjusting hole and an adjusting glove matched with the adjusting hole.
In some optional embodiments, the stimulus presentation module comprises at least one of a light emitting device, a sound emitting device, and an odor generating device.
The animal behavioristics test system has the following advantages:
(1) the infrared detection assembly has the advantages that the visible light monitoring assembly capable of monitoring the behavior of the experimental animal under the condition of sufficient light and the infrared detection assembly capable of monitoring the behavior of the experimental animal under the dark condition are arranged, so that the requirement for monitoring the behavior and activity of the animal in the environment with sufficient light can be met, and the requirement for monitoring the behavior and activity of the animal in the dark environment can be met.
(2) Can be towards the inside anesthetic gas of inputing of behaviourology box, can monitor the behavioral activity of the experimental animals of anesthesia state, can monitor the behavioral activity of the experimental animals of awake state again, satisfied the needs to the research of the instinct fear of animal betterly.
(3) Because the light pulse stimulation module is provided, the nerve loop of the instinct fear and arousal relationship of the experimental animal can be analyzed, and therefore people can be helped to find out the neural mechanism with cross-species conservation behind the back.
(4) Because the stimulation presenting module is arranged, the precision and the effectiveness of adding stimulation are improved.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
Fig. 1 is a schematic structural diagram of an animal behavioral testing system according to an embodiment of the present invention.
Reference numerals:
a ethological box body 1,
A main casing 11, an opening 111, an adjustment hole 112, an auxiliary casing 12,
The stimulation presenting module (2) is provided with a stimulation presenting module,
a light pulse stimulation module 3, a laser generator 31, a waveform generator 32, a suspension rod 33,
A video monitoring module 4, a visible light monitoring component 41, an infrared monitoring component 42,
A control module 5,
A gas inlet pipe 6, a gas recovery pipe 7 and a gas sampling pipe 8.
Detailed Description
In order to make the technical problems solved, the technical solutions adopted and the technical effects achieved by the present invention clearer, the technical solutions of the present invention are further described below by way of specific embodiments with reference to the accompanying drawings.
The structure of an animal behavioral testing system according to an embodiment of the present invention will be described with reference to fig. 1.
As shown in fig. 1, the animal ethology testing system of the present embodiment includes an ethology box 1, a stimulation presenting module 2, an optical pulse stimulation module 3, and a video monitoring module 4, wherein the ethology box 1 is configured to accommodate an experimental animal, and the ethology box 1 is provided with an air inlet through which anesthetic gas can be input into the ethology box 1. The stimulation presenting module 2 is arranged on the ethology box body 1, the stimulation presenting module 2 is configured to present stimulation towards the experimental animal, the light output end of the light pulse stimulating module 3 extends into the ethology box body 1 and is suitable for being connected with the head of the experimental animal, the video monitoring module 4 comprises a visible light monitoring component 41 and an infrared monitoring component 42, the visible light monitoring component 41 is configured to monitor the behavior of the experimental animal under the condition of sufficient light, and the infrared monitoring component 42 is configured to monitor the behavior of the experimental animal under the dark condition.
It is understood that, in the present embodiment, the video monitoring module 4 includes a visible light monitoring component 41 capable of monitoring the behavior of the laboratory animal under the condition of sufficient light and an infrared detection component capable of monitoring the behavior of the laboratory animal under the dark condition. Therefore, the requirements of animal behavior and activity can be monitored in the environment with sufficient light, and the requirements of animal behavior and activity can be monitored in the dark environment. Especially for rodents such as mice, the activity in the dark environment better conforms to the nature of life in the natural environment, thereby better meeting the needs of animal behavioral research.
It should be noted that the fear response of the animal is dependent on the level of arousal and consciousness of the animal, and different arousal states may cause the animal to be unable to realize the arrival of dangerous stimuli in time, so that defensive response cannot be made in time, and finally the survival is threatened. The anesthesia state is considered a body state as opposed to arousal, and researchers can obtain valuable experimental data by behavioral comparison of the anesthesia state with the arousal state. In this embodiment, the anesthetic gas may be introduced into the ethological box 1, so as to achieve anesthesia of the experimental animal. That is to say, the animal behavioural test system of the embodiment can monitor the behavioural activity of the experimental animal in the anesthesia state and the behavioural activity of the experimental animal in the waking state, and better meets the requirement of the research on the instinctive fear of the animal.
Because the light output end of the light pulse stimulation module 3 is connected with the head of the experimental animal, the bidirectional regulation and control of the specific brain area of the experimental animal can be realized, and the specific neuron is activated or inhibited, and then the change of the mouse behavior is observed through the video monitoring module 4, so that the experimental animal can analyze the neural loop of the instinct fear and arousal relationship, thereby being beneficial to people to find out the neural mechanism with cross-species conservation behind the back, and benefiting patients with fear-related diseases such as post-traumatic stress disorder, anxiety disorder and the like.
In the invention, the stimulation presenting module 2 may always add a specific certain stimulation to the behavioural box 1, or the stimulation presenting module 2 may be connected to an external control module 5, and the control module 5 may adjust the type of stimulation added to the behavioural box 1 by the stimulation presenting module 2 according to specific experimental requirements. Therefore, compared with artificial stimulation addition in the prior art, the animal behavior test system can improve the precision and effectiveness of stimulation addition.
In addition, preferably, the animal behavioural testing system may further comprise a control module 5, which is respectively communicated with the stimulation presenting module 2, the optical pulse stimulation module 3 and the video monitoring module 4 to transmit data. The control module 5 plays a role of data control and processing center, and provides setting, outputting and controlling of experimental stimulation parameters according to specific experimental requirements through computer programming. That is to say, when the control module 5 exists, the stimulus added to the experimental animal by the stimulus presenting module 2 is always under the control of the control module 5, so that the accuracy and effectiveness of adding the stimulus are improved. For example, the conventional stimulation for inducing the instinct fear response of the mouse can realize automatic stimulation triggering through a program, thereby improving the accuracy of experimental stimulation.
It should be added and explained that the materials of the behavioural box body 1 can be selected from methyl methacrylate materials, stainless steel, glass and other materials according to experimental requirements, all parts of the joints of the behavioural box body 1 are directly embedded together in the production process, so that the sealing performance is ensured, and the anesthetic gas can be prevented from leaking from the behavioural box body 1, thereby better solving the problem that the anesthetic gas in the prior art is easy to diffuse and the personal safety of experimental researchers is influenced. In addition, the visible light monitoring component 41 may be selected from a conventional camera, and the infrared monitoring component 42 may be selected from an infrared camera. The specific models of the visible light monitoring component 41 and the infrared monitoring component 42 are not limited, and both can be selected according to actual needs.
In some embodiments, as shown in fig. 1, the behavioural box 1 comprises a main box 11 and an auxiliary box 12, at least one side of the main box 11 is a light-transmitting sheet, the visible light monitoring component 41 is disposed corresponding to the light-transmitting sheet of the main box 11, the auxiliary box 12 is communicated with the main box 11, and the volume of the auxiliary box 12 is smaller than the volume of the main box 11. At least one side of the auxiliary box 12 is an infrared filter, and the infrared monitoring component 42 is disposed corresponding to the infrared filter of the auxiliary box 12.
It can be understood that, because the volume of the auxiliary box 12 is smaller than the volume of the main box 11, when the stimulation presenting module 2 presents visual stimulation inducing the experimental animal under dark conditions, the experimental animal can present the reaction of returning to the nest (i.e. entering the interior of the auxiliary box 12) at a high probability, at this time, because at least one side of the auxiliary box 12 is the infrared filter, the infrared monitoring component 42 is arranged corresponding to the infrared filter of the auxiliary box 12, so the infrared monitoring component 42 can well monitor the behavior and activity of the experimental animal in real time. Under the condition of sufficient light, the experimental animal is greatly recorded to move in the main box body 11 with a larger volume, and at the moment, the visible light monitoring component 41 can monitor the behavior and the activity of the experimental animal in real time. Therefore, the main box body 11 and the auxiliary box body 12 are designed in a partition mode, and requirements of various experimental conditions and stimulation can be well met.
It should be noted that, in some embodiments, each side of the main housing 11 is a light-transmitting sheet. Therefore, the visible light monitoring assembly 41 can monitor the experimental animal without dead angles, and is beneficial to the behavioral research of the experimental animal. Of course, in other embodiments, the light-transmitting sheet on the main box 11 can be adjusted according to practical situations, and is not limited to the above description.
In some alternative embodiments, as shown in fig. 1, the auxiliary case 12 is a rectangular parallelepiped, and all the side surfaces of the auxiliary case 12 are infrared filters. Therefore, the infrared monitoring assembly 42 can monitor the experimental animal without dead angles, and is beneficial to the research of the behavioristics of the experimental animal. Of course, in other embodiments, the infrared filter on the auxiliary box 12 can be adjusted according to practical situations, and is not limited to the above description.
In some embodiments, as shown in fig. 1, an opening 111 is provided on the top wall of the main housing 11, and the stimulation presentation module 2 is a screen fitted at the opening 111. From this, on the one hand be favorable to amazing the module 2 of presenting to send amazing towards experimental animals betterly, on the other hand, opening 111 can regard as the mouth of putting into of placing experimental animals, has reduced the hole structure on the main tank body 11 like this, has improved the sealing performance of main tank body 11.
Advantageously, the edge of the opening 111 is designed with soft silica gel, and the stimulation presenting module 2 can ensure the tightness of the main box body 11 after covering the opening 111, thereby avoiding the phenomenon that the leakage of anesthetic gas affects experimenters, and improving the safety of the animal behavior testing system.
It should be added here that when the stimulation presenting module 2 is a screen, a temperature sensor can be added to the stimulation presenting module 2 to monitor the temperature inside the ethological box 1, or a camera is installed on the stimulation presenting module 2 to shoot the experimental animal.
In some embodiments, as shown in fig. 1, the animal ethology testing system further includes a gas inlet pipe 6, a gas recovery pipe 7, and a gas sampling pipe 8, the gas inlet pipe 6 is communicated with the ethology box 1, the gas inlet pipe 6 is configured to input experimental gas into the ethology box 1, the gas recovery pipe 7 is communicated with the ethology box 1, the gas recovery pipe 7 is configured to recover gas inside the ethology box 1, the gas sampling pipe 8 is communicated with the ethology box 1, and the gas sampling pipe 8 is configured to extract gas inside the ethology box 1 and output the gas to an external detection device. Therefore, the stable input of anesthetic gas into the ethology box body 1 can be ensured, the stable recovery of gas in the ethology box body 1 can be ensured, the phenomenon of gas leakage in the ethology box body 1 is avoided, and the use safety of the animal ethology test system is ensured. And the existence of gas sampling pipe 8 can realize the real-time supervision of the inside gas concentration of behavioural box 1, composition, and what kind of anesthesia state that accurate monitoring experiment animal is in is favorable to the research to animal behavioural.
In some specific embodiments, as shown in fig. 1, the gas recovery tube 7 has a tube diameter greater than that of the gas inlet tube 6, and the gas inlet tube 6 has a tube diameter greater than that of the gas sampling tube 8. It can be understood that, the gas inside the behavior box 1 needs to be recovered after the experiment is finished under general conditions, and the pipe diameter of the gas recovery pipe 7 is large, so that the rapid recovery of the gas inside the behavior box 1 is facilitated, and the experiment efficiency is improved. And the pipe diameter of the gas inlet pipe 6 is relatively small, so that the flow of the anesthetic gas towards the behavioural box 1 can be well controlled, and the control on the anesthetic state of the experimental animal is better realized. In addition, the diameter of the gas sampling pipe 8 is small, and the influence on the gas environment in the ethology box body 1 is small in the sampling process, namely the diameter of the gas sampling pipe 8 is small, so that stable sampling of gas in the ethology box body can be guaranteed, the influence on the gas concentration in the ethology box body 1 can be reduced, and the possibility that the anesthesia state of an experimental animal is changed greatly in the experimental process is reduced. Of course, in other embodiments, the pipe diameters of the three pipes may be designed according to actual needs, and are not limited to the above definition.
In some embodiments, as shown in fig. 1, the light pulse stimulation module 3 includes a laser generator 31 and a waveform generator 32, the laser generator 31 is connected to the head of the experimental animal through an optical fiber, and the waveform generator 32 is connected to the laser generator 31 to adjust the laser parameters emitted by the laser generator 31. Thereby. The waveform generator 32 adjusts 113 the intensity and light output form of the laser generated by the laser generator 31, so that the photostimulation parameters required by the optogenetic experiment, such as duty ratio, amplitude, wave width and the like, can be realized. The specific models of the laser generator 31 and the waveform generator 32 are not limited, and the specific models of the laser generator 31 and the waveform generator 32 may be selected according to actual conditions.
In some specific embodiments, as shown in fig. 1, the optical pulse stimulation module 3 further comprises a suspension rod 33. One end of the suspension rod 33 is connected to the inner wall of the ethology box body 1, the other end of the suspension rod 33 is provided with a universal joint, and the optical fiber penetrates through the universal joint and then is connected with the head of the experimental animal. Therefore, the optical fiber connected with the brain bag of the experimental animal cannot influence the free movement of the experimental animal due to the weight, and the phenomenon of optical fiber winding can be prevented.
In some alternative embodiments, as shown in fig. 1, the behavior box 1 is provided with an adjusting hole 112 and an adjusting glove fitted on the adjusting hole. Therefore, if the experiment needs manual intervention for some activities and postures of the experimental animal, the hand can be extended into the behavior box body 1 through the adjusting hole 112 for adjustment, so that the animal behavior research is facilitated. The existence of adjustment gloves when guaranteeing behavioural science box 1's leakproofness, can avoid experimental animal to scratch or bite laboratory glassware.
In some alternative embodiments, the stimulus presentation module 2 comprises at least one of a light emitting device, a sound emitting device, and an odor generating device. Therefore, various stimuli can be sent to the experimental animal, and the application range of the animal behavioural test system is expanded.
In some optional embodiments, the animal behavior test system can also ensure a sound wave positioning device, a fluorescence imaging device and the like to realize real-time monitoring on the experimental animal.
Example (b):
the specific structure of an animal behavioral system according to one embodiment of the present invention will be described below with reference to fig. 1.
The animal ethology system comprises an ethology box body 1, a stimulation presenting module 2, an optical pulse stimulation module 3, a video monitoring module 4 and a control module 5, wherein the ethology box body 1 is configured to accommodate experimental animals, a gas inlet is formed in the ethology box body 1, and anesthetic gas can be input into the ethology box body 1 through the gas inlet. The stimulation presenting module 2 is arranged on the ethology box body 1, the stimulation presenting module 2 is configured to present stimulation towards the experimental animal, the light output end of the light pulse stimulating module 3 extends into the ethology box body 1 and is suitable for being connected with the head of the experimental animal, the video monitoring module 4 comprises a visible light monitoring component 41 and an infrared monitoring component 42, the visible light monitoring component 41 is configured to monitor the behavior of the experimental animal under the condition of sufficient light, and the infrared monitoring component 42 is configured to monitor the behavior of the experimental animal under the dark condition. The control module 5 communicates with the stimulation presenting module 2, the optical pulse stimulation module 3, and the video monitoring module 4 respectively to transmit data.
The ethology box 1 comprises a main box body 11 and an auxiliary box body 12, wherein the main box body 11 is formed into a cuboid, the front side surface of the main box body 11 is a light-transmitting sheet, and the visible light monitoring component 41 is arranged corresponding to the light-transmitting sheet of the main box body 11. An opening 111 is arranged on the top wall of the main box body 11, and the stimulation presenting module 2 is a screen matched at the opening 111. The side wall of the ethological box 1 is provided with an adjusting hole 112. The auxiliary case 12 is communicated with the main case 11, and the volume of the auxiliary case 12 is smaller than that of the main case 11. The auxiliary case 12 is formed in a rectangular parallelepiped, and each side surface of the auxiliary case 12 is an infrared transmitting sheet.
The ethology box body 1 is provided with a gas inlet pipe 6, a gas recovery pipe 7 and a gas sampling pipe 8 which are communicated with the ethology box body 1, the gas inlet pipe 6 is configured to input experimental gas towards the interior of the ethology box body 1, the gas recovery pipe 7 is configured to recover gas inside the ethology box body 1, the gas sampling pipe 8 is communicated with the ethology box body 1, and the gas sampling pipe 8 is configured to extract gas inside the ethology box body 1 and output the gas to an external detection device. The pipe diameter of the gas recovery pipe 7 is larger than that of the gas inlet pipe 6, and the pipe diameter of the gas inlet pipe 6 is larger than that of the gas sampling pipe 8.
The light pulse stimulation module 3 comprises a laser generator 31, a waveform generator 32 and a suspension rod 33, wherein the laser generator 31 is connected with the head of the experimental animal through an optical fiber, and the waveform generator 32 is connected with the laser generator 31 so as to adjust laser parameters emitted by the laser generator 31. One end of the suspension rod 33 is connected to the inner wall of the ethology box body 1, the other end of the suspension rod 33 is provided with a universal joint, and the optical fiber penetrates through the universal joint and then is connected with the head of the experimental animal.
The animal behavioral system of the embodiment can be used for behavioral research of instinct fear, arousal and anesthesia states, and can also be used for researching behavioral characterization of animal models with mental diseases such as epilepsy and Alzheimer's disease.
The animal behavioristics system of this example has the following characteristics:
(1) the airtightness is fully considered in the production process, when the box body is used for related researches on anesthesia, the behavioristics box body 1 can be filled with gas anesthetics such as isoflurane and the like, and the gas sampling tube 8 is matched, so that the personal safety of experimenters and the accuracy of gas concentration are ensured.
(2) The animal behavior monitoring device is provided with a common visible light monitoring component 41 and is combined with an infrared monitoring component 42, so that the requirement of experimenters for monitoring animal behavior activities in a dark environment can be met. Is particularly suitable for experimental animals which move in dark environment and better accord with the nature of life in natural environment.
(3) The stimulation applied towards the experimental animal can be triggered automatically by the program of the control module 5, improving the accuracy of the experimental stimulation.
(4) The optical pulse stimulation module 3 is arranged, the combination of conventional ethology experiments and the optogenetic technology is considered, the characteristics of the optogenetic technology can be fully exerted, and specific behavior indexes are considered under the state that experimental animals move freely.
In the description herein, references to the description of "some embodiments," "other embodiments," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The above description is only a preferred embodiment of the present invention, and for those skilled in the art, the present invention should not be limited by the description of the present invention, which should be interpreted as a limitation.
Claims (10)
1. An animal behavioral testing system, comprising:
the ethological box body (1), the ethological box body (1) is configured to contain experimental animals, a gas inlet is arranged on the ethological box body (1), and anesthetic gas can be input into the ethological box body (1) through the gas inlet;
a stimulus presentation module (2), the stimulus presentation module (2) being provided on the behavioural box (1), the stimulus presentation module (2) being configured to present a stimulus towards an experimental animal;
the optical output end of the optical pulse stimulation module (3) extends into the ethology box body (1) and is suitable for being connected with the head of an experimental animal;
the video monitoring module (4), the video monitoring module (4) includes visible light monitoring subassembly (41) and infrared monitoring subassembly (42), visible light monitoring subassembly (41) are configured to the action of experimental animal under the sufficient condition of monitoring light, infrared monitoring subassembly (42) are configured to the action of experimental animal under the dark condition.
2. Animal ethology test system according to claim 1, characterized in that the ethology box (1) comprises:
the visible light monitoring device comprises a main box body (11), wherein at least one side surface of the main box body (11) is provided with a light transmitting sheet, and the visible light monitoring component (41) is arranged corresponding to the light transmitting sheet of the main box body (11);
the auxiliary box body (12), auxiliary box body (12) with main box body (11) intercommunication, just the volume of auxiliary box body (12) is less than the volume of main box body (11), at least one side of auxiliary box body (12) is infrared filter, infrared monitoring subassembly (42) correspond auxiliary box body (12) infrared filter sets up.
3. The animal ethology testing system according to claim 2, wherein an opening (111) is provided on the top wall of the main box (11), and the stimulus presentation module (2) is a screen fitted at the opening (111).
4. The animal ethology testing system according to claim 2, wherein the auxiliary box (12) is a cuboid, and all sides of the auxiliary box (12) are infrared filters.
5. The animal behavioral testing system according to claim 1, characterized in that the animal behavioral testing system further comprises:
a gas inlet tube (6), the gas inlet tube (6) being in communication with the gas inlet, the gas inlet tube (6) being configured to input anesthetic gas into the ethological box (1);
a gas recovery pipe (7), the gas recovery pipe (7) being in communication with the ethological tank (1), the gas recovery pipe (7) being configured to recover gas inside the ethological tank (1);
a gas sampling pipe (8), the gas sampling pipe (8) with the behavioural box (1) intercommunication, the gas sampling pipe (8) are configured to extract the inside gas of behavioural box (1) and export to external detection device.
6. The animal behavioristics testing system according to claim 5, wherein a pipe diameter of the gas recovery pipe (7) is greater than a pipe diameter of the gas inlet pipe (6), and a pipe diameter of the gas inlet pipe (6) is greater than a pipe diameter of the gas sampling pipe (8).
7. Animal behavioristic testing system as claimed in claim 1, characterized in that said light pulse stimulation module (3) comprises:
the laser generator (31), the said laser generator (31) couples to head of the experimental animal through the optic fibre;
the waveform generator (32), the waveform generator (32) is connected with the laser generator (31) to adjust the laser parameters emitted by the laser generator (31).
8. The animal ethology testing system according to claim 7, wherein the light pulse stimulation module (3) further comprises a suspension rod (33), one end of the suspension rod (33) is connected to the inner wall of the ethology box body (1), the other end of the suspension rod (33) is provided with a universal joint, and the optical fiber is connected with the head of the experimental animal after passing through the universal joint.
9. The animal ethology test system according to claim 1, wherein the ethology box (1) is provided with an adjusting hole (112) and an adjusting glove fitted on the adjusting hole.
10. The animal behavioristic testing system according to claim 1, wherein the stimulus presentation module (2) comprises at least one of a light emitting device, a sound emitting device and an odor generating device.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910483279.6A CN112022091A (en) | 2019-06-04 | 2019-06-04 | Animal behavioristics test system |
| PCT/CN2019/120889 WO2020244171A1 (en) | 2019-06-04 | 2019-11-26 | Animal behavioral test system |
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| CN201910483279.6A CN112022091A (en) | 2019-06-04 | 2019-06-04 | Animal behavioristics test system |
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| CN112022091A true CN112022091A (en) | 2020-12-04 |
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| CN201910483279.6A Pending CN112022091A (en) | 2019-06-04 | 2019-06-04 | Animal behavioristics test system |
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| CN (1) | CN112022091A (en) |
| WO (1) | WO2020244171A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113875617A (en) * | 2021-10-18 | 2022-01-04 | 中国科学院深圳先进技术研究院 | Behaviourology experimental device capable of adjusting visual danger stimulation intensity |
| WO2024098392A1 (en) * | 2022-11-11 | 2024-05-16 | 中国科学院深圳先进技术研究院 | Apparatus for detecting fear behavior of animal for olfactory stimulus |
| WO2024108651A1 (en) * | 2022-11-25 | 2024-05-30 | 中国科学院深圳理工大学(筹) | Apparatus for monitoring in-vivo intestinal motility in real time |
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| CN203852448U (en) * | 2014-04-24 | 2014-10-01 | 温州医科大学附属第二医院 | Inhalation anesthesia exposing and monitoring device |
| CN104382606A (en) * | 2014-11-12 | 2015-03-04 | 中国科学院深圳先进技术研究院 | Animal behavior test system and method |
| KR101638605B1 (en) * | 2014-11-28 | 2016-07-11 | 기초과학연구원 | Test apparatus and method of recognition function of target |
| CN104523232B (en) * | 2014-12-26 | 2018-07-06 | 中国科学院深圳先进技术研究院 | A kind of meiofauna fear behavioral value system |
| US20170000081A1 (en) * | 2015-06-30 | 2017-01-05 | Mousera, Inc | System and method of automatic classification of animal behaviors |
| CN105532489B (en) * | 2015-12-10 | 2018-12-21 | 中国科学院深圳先进技术研究院 | A kind of animal experiment device |
| CN106264569B (en) * | 2016-08-10 | 2020-03-06 | 深圳先进技术研究院 | Shared emotion nerve experiment system based on observational fear acquisition |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113875617A (en) * | 2021-10-18 | 2022-01-04 | 中国科学院深圳先进技术研究院 | Behaviourology experimental device capable of adjusting visual danger stimulation intensity |
| WO2023065498A1 (en) * | 2021-10-18 | 2023-04-27 | 中国科学院深圳先进技术研究院 | Behavioral experimental apparatus capable of adjusting the intensity of visual threatening stimulus |
| WO2024098392A1 (en) * | 2022-11-11 | 2024-05-16 | 中国科学院深圳先进技术研究院 | Apparatus for detecting fear behavior of animal for olfactory stimulus |
| WO2024108651A1 (en) * | 2022-11-25 | 2024-05-30 | 中国科学院深圳理工大学(筹) | Apparatus for monitoring in-vivo intestinal motility in real time |
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