CN112021317A

CN112021317A - Insecticidal composition containing pyriproxyfen and monosultap and application thereof

Info

Publication number: CN112021317A
Application number: CN202011074337.9A
Authority: CN
Inventors: 苑志军; 王大中; 蒙羽涵; 徐海燕; 张芝平; 郑昉; 王晓辉; 王鸿宾
Original assignee: Shanghai Shengnong Pesticide Co Ltd
Current assignee: Shanghai Shengnong Pesticide Co Ltd
Priority date: 2020-10-09
Filing date: 2020-10-09
Publication date: 2020-12-04

Abstract

The invention discloses an insecticidal composition containing pyriproxyfen and monosultap and application thereof, wherein the insecticidal composition comprises pyriproxyfen and monosultap, and the mass ratio of the pyriproxyfen to the monosultap is 1: 1-60. The insecticidal composition has the advantages that the lasting period is prolonged, the two effective components are compounded to achieve the synergistic effect, and the use amount of the two effective components is reduced compared with that of a single agent, so that the insecticidal effect is improved, the generation of resistance is slowed down, the environmental pressure is reduced, and the insecticidal composition is low in toxicity, low in residue and free of public nuisance.

Description

Insecticidal composition containing pyriproxyfen and monosultap and application thereof

Technical Field

The invention relates to the technical field of pesticides for plant chemical protection, in particular to a pesticide composition containing pyriproxyfen and monosultap and application thereof.

Background

Lepidoptera pests are harmful pests, and the common main species of the lepidoptera comprise rice leaf rollers, asparagus caterpillars, prodenia litura, peach fruit borers, cotton bollworms, diamond back moths, diaphania cucullata, bean pod borers and the like. Once serious resistance is generated, great loss, confusion and improvement of control cost are brought to farmers. Lepidopteran pests are completely metamorphic insects (eggs → larvae → pupae → adults), and are of a wide variety and distributed everywhere. Currently, about 8000 lepidoptera insects are known in China, most larvae are harmful to various plants, and the larger larvae often eat plant leaves or bore branches completely. Smaller-sized plants are usually damaged by rolling leaves, decorating leaves, knotting sheaths, spinning and knotting nets or drilling into plant tissues for eating, and adults mostly take nectar and the like as supplementary nutrition, so that the crops are directly killed when the damage is serious. The prior lepidoptera prevention and control agents are various, but in the process of preventing and controlling the old lepidoptera, various problems exist in the use of the agents, so that the lepidoptera pest has high resistance.

The liriomyza species are many in variety, small in size, usually about 2mm, and about 160 of them can be harmful to cultivated crops and ornamental plants. The generations of liriomyza insects are obviously overlapped, and researches show that more than 10 generations of liriomyza americana can occur all the year around, and 1 generation can be completed in 12 days at the shortest time under the appropriate environmental conditions. Liriomyza sativae is a pest which is seriously harmful and difficult to control on kidney beans in recent years. The liriomyza sativae is seriously harmful, the distribution range of the liriomyza sativae is wide, at present, more than 20 liriomyza sativae in China mainly eat bean leaves and tender pods, bent tunnels can be left after eating, photosynthesis of beans is affected, leaves of bean plants fall off when the liriomyza sativae is seriously damaged, flower buds and fruits are damaged, and the yield and commodity value of the beans are seriously affected. The hazard mode of the liriomyza sativae is mainly that the liriomyza sativae is eaten by submerging in leaves, petioles and pods, which brings certain difficulties to prevention and control. In recent years, the pesticide resistance of liriomyza sativae is continuously improved, researches show that the pesticide resistance level of the liriomyza sativae to abamectin is improved from 13.50 times to 36.05 times, and the pesticide resistance control is a great problem.

Monosultap (Monosultap) is an artificially synthesized nereistoxin analogue, and belongs to nereistoxin insecticides. The monosultap is quickly converted into nereistoxin or dihydronereistoxin after entering the insect body, and the nereistoxin or the dihydronereistoxin is a competitive inhibitor of acetylcholine, acts on acetylcholine receptors of a nervous system, blocks synaptic signal conduction, and paralyzes the insect until death. The monosultap has strong stomach toxicity and contact killing effect, also has certain systemic and fumigation effect, has a wide insecticidal spectrum, and is widely applied to pest control of crops such as rice, vegetables, oranges, tobacco, tea trees and the like.

Pyriproxyfen (Pyriproxyfen) is an insect growth regulator capable of disturbing the normal growth of insects, belongs to a novel insecticide of juvenile hormone analogues, and has the characteristics of systemic transfer activity, low toxicity (for example, low toxicity to fishes), long lasting period, safety to crops and small influence on the ecological environment. In addition, pyriproxyfen has good control effect on pear psylla, bemisia tabaci, scale insect, diamond back moth, beet armyworm, prodenia litura, citrus psylla, thrips and the like; meanwhile, the pyriproxyfen has good control effect on sanitary pests such as flies, mosquitoes and the like, because the pyriproxyfen has the effect of inhibiting mosquitoes and fly larvae from pupating and eclosion, most of the mosquitoes and fly larvae die in the pupation stage and cannot eclosion after contacting the pyriproxyfen.

In conclusion, the pyriproxyfen and the monosultap are safe and efficient environment-friendly pesticides. However, when the pesticide composition is used as a single agent, the insecticidal spectrum is limited, for example, the pyriproxyfen has better control effect on scale insects, and the monosultap has poorer control effect on scale insects than the pyriproxyfen.

Chinese patent publication No. CN107347886A discloses a field tank-mixed pesticide for controlling rice pests, which is prepared by mixing two pesticide preparations, the active ingredients of the two preparations are monosultap and pyriproxyfen respectively, but the stability of different dosage forms of tank-mixing needs to be improved, for example, the stability of Soluble Powder (SP) using monosultap as the active ingredient and Emulsifiable Concentrate (EC) using pyriproxyfen as the active ingredient after tank-mixing is poor, and the pesticide cannot be used after tank-mixing.

Disclosure of Invention

The invention aims to provide an insecticidal composition containing pyriproxyfen and monosultap and application thereof, aiming at the defects in the prior art, the insecticidal composition has better stability, shows environment-friendly and efficient compound synergistic insecticidal effect, can be widely applied to agricultural plant production, and is mainly applied to the prevention and control of lepidoptera and homoptera pests of crops such as vegetables, fruit trees, rice, cotton and the like.

In order to achieve the purpose, the invention adopts the technical scheme that:

the invention provides an insecticidal composition containing pyriproxyfen and monosultap, which comprises pyriproxyfen and monosultap, wherein the mass ratio of the pyriproxyfen to the monosultap is 1: 1-60.

Preferably, the mass ratio of the pyriproxyfen to the monosultap is 1: 2-50.

Preferably, the mass ratio of the pyriproxyfen to the monosultap is 1: 5-40.

Preferably, the mass ratio of the pyriproxyfen to the monosultap is 1: 9-30.

Preferably, the mass ratio of the pyriproxyfen to the monosultap is 1: 10-20.

It is worth mentioning that the mass ratio of the pyriproxyfen and the monosultap can be further adjusted within a reasonable range according to specific application conditions. More preferably 1:55, 1:50, 1:45, 1:40, 1:39, 1:38, 1:37, 1:36, 1:35, 1:34, 1:33, 1:32, 1:31, 1:30, 1:29, 1:28, 1:27, 1:26, 1:25, 1:24, 1:23, 1:22, 1:21, 1:20, 1:19, 1:18, 1:17, 1:16, 1:15, 1:14, 1:13, 1:12, 1:11, 1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, etc.

Preferably, the mass sum of the pyriproxyfen and the monosultap accounts for 1-99% of the total mass of the insecticidal composition.

Preferably, the mass sum of the pyriproxyfen and the monosultap accounts for 5-95% of the total mass of the insecticidal composition.

Preferably, the mass sum of the pyriproxyfen and the monosultap accounts for 10-80% of the total mass of the insecticidal composition.

Preferably, the dosage form of the insecticidal composition comprises, but is not limited to, nano-microemulsion, soluble liquid agent, soluble powder, wettable powder, soluble granule, aqueous emulsion, aqueous suspension, missible oil and emulsion powder.

It is worth to be noted that the pyriproxyfen and the monosultap are used as effective components, and the pesticide auxiliary agents used for processing the pesticide formulations known to the person skilled in the art can be processed into the pesticide formulations according to different proportions expressed by the invention and by a pesticide formulation processing method known to the person skilled in the art.

The second aspect of the invention provides an application of the insecticidal composition containing pyriproxyfen and monosultap in controlling crop pests, wherein the crop pests are lepidoptera pests, homoptera pests or underground pests, and the insecticidal composition is particularly applied to controlling the pests of chilo suppressalis, scale insects, leaf miners or leaf miners on crops.

By adopting the technical scheme, compared with the prior art, the invention has the following technical effects:

1. the insecticidal composition can control or delay the drug resistance of pests, is environment-friendly, expands the pest control spectrum, can control various pests simultaneously, has obvious compound synergistic effect, can reduce the pesticide application frequency, and achieves the effect of one-time pesticide application and simultaneous control of various pests.

2. The insecticidal composition has the advantages that the lasting period is prolonged, the two effective components are compounded to achieve the synergistic effect, and the use amount of the two effective components is reduced compared with that of a single agent, so that the insecticidal effect is improved, the generation of resistance is slowed down, the environmental pressure is reduced, and the insecticidal composition is low in toxicity, low in residue and free of public nuisance.

Detailed Description

The present invention is further illustrated by the following examples, which are not to be construed as limiting the invention.

It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict.

The test devices, materials, reagents and the like used in the following examples are commercially available unless otherwise specified.

Detection of co-toxicity coefficients of pyriproxyfen and monosultap in different ratios on rice stem borer, scale insect, leaf miner and leaf miner

(1) Determination of co-toxicity coefficient of pyriproxyfen and monosultap in different ratios to rice stem borer

In order to explore the synergistic effect, the additive effect or the antagonistic effect of the compounded pyriproxyfen and monosultap, relevant experiments of formula screening are carried out on pyriproxyfen and monosultap, Chilo suppressalis causing serious harm to rice production in China are selected as implementation objects of indoor toxicity measurement, and specific measurement conditions and test results are as follows:

according to the data provided by the ministry of agriculture drug inspection, the recommended dosage of 100g/L pyriproxyfen emulsifiable concentrate for preventing and controlling scale insects in citrus is 1000-1500 times liquid, and the recommended dosage of monosultap for preventing and controlling rice stem borer is 36-54 g/mu of effective component. Therefore, the ratio of the two agents for the indoor toxicity measurement is set to be 1:1, 1:2, 1:5, 1:10, 1:20, 1:30, 1:40, 1:50 and 1: 60. Except the compound treatment of two medicaments with different concentrations, two single-agent treatments of pyriproxyfen and monosultap and blank control acetone are additionally arranged.

The indoor bioassay method comprises the following steps: capillary micro-drop method. A capillary micro dropper with the volume of 1.0 mu L is adopted, and the test insects are larvae of the rice stem borer at 3 rd age. Dripping liquid medicine with various concentrations on the back of chilo suppressalis larvae one by using a capillary micro dropper, dripping 30 larvae into each type of larvae for processing, putting 5 test insects into a culture dish with the diameter of 9 cm, putting a small amount of feed into the culture dish for the test insects to eat, and dripping 30 larvae into acetone to serve as a control group. And (5) placing the treated test insects in a feeding room, and checking the mortality rate of the test insects after 48 hours. And (3) carrying out statistical treatment on the data obtained by the test by using SPSS software to obtain a toxicity regression equation, lethal medium concentration, correlation coefficient and the like, and solving the cotoxicity coefficient by using a Sun cloud Peel method. The joint virulence of the mixed preparation is expressed by adopting a Sunweier cotoxicity coefficient method:

theoretical virulence index of a compound formulation TTI ═ Sigma (virulence index ATI of a particular agent x the percentage of active ingredient in the compound)

The judgment standard of the synergistic effect of the two effective components after compounding is as follows:

when the CTC is more than 120, the CTC is synergistic, when the CTC is less than 80, the CTC is antagonistic, and 80-120 is additive.

The experiment determines that LC50 of the pyriproxyfen to chilo suppressalis is 13.88 mu g/mL, and the toxicity index of the pyriproxyfen to chilo suppressalis is set to be 100. The virulence index and the co-intensity coefficient of each treatment to chilo suppressalis are shown in table 1:

TABLE 1 indoor toxicity test results of pyriproxyfen and monosultap in different ratios to Chilo suppressalis

As can be seen from the data in table 1, when pyriproxyfen and monosultap are compounded according to different proportions, the co-toxicity coefficient of all the compounded formulas is greater than 120 except that a: B is 1:60, which indicates that the formulas achieve obvious synergistic effect. The composition shows that after two effective components of pyriproxyfen and monosultap are compounded, a good synergistic effect can be achieved. When the ratio of the pyriproxyfen to the monosultap is 1:10, the co-toxicity coefficient reaches 196.99, which is the highest value of the co-toxicity coefficients of all formulas, and the synergistic effect is most obvious.

(2) Determination of co-toxicity coefficient of pyriproxyfen and monosultap in different ratios to citrus scale insects

In order to determine whether the combination of pyriproxyfen and monosultap has a synergistic effect, an additive effect or an antagonistic effect on the control of homoptera scale insects, the formula in example 1 is used for carrying out indoor toxicity determination on citrus scale insects, and the specific method comprises the following steps:

the test adopts a spraying method, and refers to agriculture of the people's republic of ChinaIndustry Standard NY/T1154.9-2008. The test insects are scale insects nymphs collected in a citrus orchard and continuously bred and bred indoors. Selecting healthy and consistent-age Citrus scale insect nymphs, treating 4 culture dishes with 40 nymphs each, repeating for 4 times, and spraying with a potter spray tower at 1.47 × 10⁵Spraying under Pa pressure, spraying one dish each time, spraying 1mL of liquid in each dish, taking out the culture dish after settling for 1min, putting the treated culture dish into a container with the temperature of (25 +/-1) DEG C, the relative humidity of 60-80% and the illumination period of L: culturing in an illumination incubator for 16: 8h, and spraying clean water with the same amount as that of the blank control. And (5) processing for 72 hours to check the death condition of the test insects, recording the total number of the insects and the number of the dead insects, and calculating the death rate. The LC50 values for each drug were determined by linear regression analysis between the mortality probability values and the log values of the concentrations in the series, and the cotoxicity coefficient (CTC) of the mixture was calculated according to the formula above and according to the grandsino method. The experiment proves that the lethal middle concentration of the pyriproxyfen to the citrus scale insects is 16.58 mug/mL, and the virulence index of the pyriproxyfen to the citrus scale insects is set to be 100. Virulence indices and cotoxicity coefficients for citrus scale insects for each treatment are shown in table 2:

TABLE 2 indoor toxicity test results of pyriproxyfen and monosultap in different ratios for citrus scale insect

As can be seen from the data in Table 2, the co-toxicity coefficients of the pyriproxyfen and the monosultap compounded in different proportions are all larger than 120, namely the two active ingredients have synergistic effects on preventing and controlling citrus scale insect nymphs, and the compound of the pyriproxyfen and the monosultap is reasonable and feasible. When the mass ratio of the pyriproxyfen to the monosultap is 1:20, the cotoxicity coefficient is 229.55, and the optimal synergistic effect is achieved.

(3) Determination of co-toxicity coefficient of pyriproxyfen and monosultap in different ratios to citrus leaf miner

In order to determine whether the combination of pyriproxyfen and monosultap has a synergistic effect, an additive effect or an antagonistic effect on the control of homoptera scale insects, the formula in the embodiment 1 is used for carrying out indoor toxicity determination on citrus leaf miner, and the specific method comprises the following steps:

the test was carried out by the drop method. The test insects are larvae of the citrus leaf miner collected in a citrus orchard and bred indoors. Selecting healthy citrus leaf miner larvae with consistent age, putting the citrus leaf miner larvae into a culture dish with citrus leaves, processing the test insects head by using a micro-dropping instrument, dropping 1 mu L of medicament into each test insect, dropping the medicament onto the breast and back plates of the test insects, taking acetone drops as a control group for each 15 processed larvae, repeating the processing for 3 times, and putting the processed test insects into an artificial climate chamber for feeding for 24 hours, wherein the temperature of the processed test insects is 25 +/-1 ℃, the relative humidity of the processed test insects is 70 +/-5%, and the illumination ratio of the processed test insects is 12h:12 h. And (5) processing for 24 hours to check the death condition of the test insects, using fine hairbrush to lightly touch the test insects, regarding no reaction or only slight reaction as the test insects, recording the total number of the insects and the number of dead insects, and calculating the death rate. And (3) carrying out statistical treatment on the data obtained by the test by using SPSS software to obtain a toxicity regression equation, lethal medium concentration, correlation coefficient and the like, and solving the cotoxicity coefficient by using a Sun cloud Peel method.

The experiment proves that the lethal middle concentration of the pyriproxyfen to the citrus leaf miner is 3.17 mu g/mL, and the virulence index of the pyriproxyfen to the citrus scale insect is set to be 100. Virulence indices and cotoxicity coefficients for citrus scale insects for each treatment are shown in table 3:

table 3 indoor toxicity test results of pyriproxyfen and monosultap in different ratios for citrus leaf miner

As can be seen from the data in Table 3, the co-toxicity coefficients of the pyriproxyfen and the monosultap in different proportions except 1:60 are all larger than 120, namely the two effective components have synergistic effect on preventing and controlling citrus leaf miner larvae, and the rationality and feasibility of the pyriproxyfen and monosultap compounding are demonstrated. When the mass ratio of the pyriproxyfen to the monosultap is 1:10, the cotoxicity coefficient is 229.00, and the optimal synergistic effect is achieved.

(4) Determination of co-toxicity coefficient of pyriproxyfen and monosultap in different proportions to liriomyza sativae

In order to determine whether the combination of pyriproxyfen and monosultap has a synergistic effect, an additive effect or an antagonistic effect on the control of liriomyza sativae, the formulation in example 1 is used for carrying out indoor toxicity determination on the liriomyza sativae, and the specific method comprises the following steps:

the test insects are collected from a garden of kidney beans in Shanghai Songjiang province and are raised indoors for many generations by adopting a dipping method. The formulation of example 1 was diluted to a series of concentrations with water, and 20 healthy, consistently aged larvae were picked up and placed into petri dishes with filter paper and kidney bean leaves, and 500 μ L of the formulation was dropped onto the worm using a micropipette, each treatment being repeated 3 times. After treatment, the treated larva is placed in a light incubator at the temperature of 21 +/-1 ℃, the state of the tested larva is checked after 24 hours, the larva is regarded as dead after being immobilized or incapable of normally feeding, the number of live insects and the number of dead insects are recorded, and the death rate is calculated. And (3) carrying out statistical treatment on the data obtained by the test by using SPSS software to obtain a toxicity regression equation, lethal medium concentration, correlation coefficient and the like, and solving the cotoxicity coefficient by using a Sun cloud Peel method.

The experiment proves that the lethal middle concentration of the pyriproxyfen to the citrus leaf miner is 24.07 mu g/mL, and the virulence index of the pyriproxyfen to the liriomyza sativae is set to be 100. The virulence index and cotoxicity coefficient for liriomyza sativae for each treatment are shown in table 4:

TABLE 4 indoor toxicity test results of pyriproxyfen and monosultap in different ratios for Liriomyza sativae

As can be seen from the data in Table 4, the co-toxicity coefficients of the pyriproxyfen and the monosultap compounded in different proportions are all larger than 120, namely the two effective components have synergistic effects on controlling the liriomyza sativae on the kidney beans, and the compound of the pyriproxyfen and the monosultap is reasonable and feasible. When the mass ratio of the pyriproxyfen to the monosultap is 1:10, the cotoxicity coefficient is 211.93, and the optimal synergistic effect is achieved.

Taken together, the above indoor virulence assay results show that: the pyriproxyfen and the monosultap are mixed according to different proportions, and basically have synergistic effect on chilo suppressalis, citrus leaf miner and scale insects of homoptera pests when the proportion of the pyriproxyfen to the monosultap is 1:1 to 1: 60.

Example 1

11% pyriproxyfen monosultap soluble liquid:

example 2

48% pyriproxyfen monosultap suspension:

example 3

50% pyriproxyfen monosultap dispersible oil suspension:

example 4

30% pyriproxyfen-monosultap water dispersible granule:

example 5

60% pyriproxyfen monosultap water dispersible granule:

the pyriproxyfen and the monosultap are prepared into the above 5 embodiments according to various different proportions, field pesticide effect tests for preventing and controlling rice stem borers, scale insects and underground pests are carried out, meanwhile, the control effects of the monosultap soluble powder with the concentration of 50 percent and the pyriproxyfen emulsifiable concentrate with the concentration of 100g/L are compared, and the synergistic effect after compounding is verified.

The field control effect test for the rice stem borers is carried out at the site of river pool city in Guangxi province, the time is 5 months and 15 days in 2019, 7 treatments are set, each treatment is repeated three times, test cells are randomly arranged, and the area of each cell is 8 x 4m 2. The number of chilo suppressalis larvae of 25-clump rice plants was investigated at 1d, 7d and 15d after application, respectively, and the control effect was calculated. The results of the efficacy calculations are shown in table 5:

TABLE 5 field test results of pesticide effect of pyriproxyfen, monosultap and compound preparation on Chilo suppressalis

As can be seen from the data in Table 5, the composition of pyriproxyfen and monosultap in different proportions has a significantly better effect on preventing rice stem borer than the two single agents of pyriproxyfen and monosultap, and the three formulas show synergistic effect. The control effect of each compound preparation on the 7 th day after application is higher than that of the 1 st day, the control effect of each compound preparation on the 15 th day is lower than that of the 7 th day, and the control effect of each compound preparation is obviously improved compared with that of pyriproxyfen and monosultap.

The experimental place for the field control effect of the citrus scale insects is Sichuan Meishan city, Sichuan province, the experimental time is 2019, 5 months and 26 days, six treatments are set, each treatment is repeated, experimental cells are randomly arranged, and the area of each cell is 9 x 5m². The population base number of the worms is checked before application, and the number of the remaining live worms is investigated 7d, 14d and 30d after application. 2 plants are randomly investigated in each cell, 5 leaves are respectively investigated in each cell according to the east, west, south and north directions, 40 leaves are investigated in each cell in total, the number of live insects is recorded, and the control effect is calculated. The prevention effect calculation method comprises the following steps:

control effect (%) - (1-CK)₁×Pt₁/CK₂×Pt₂)×100

Wherein CK₁Representing the population of the insects before the drug is applied to the control area; pt₁Representing the number of insect population after the treatment area is filled with the drug;

CK₂representing the number of insect population after drug administration in the control area; pt₂Representing the number of insect population before drug treatment in the treatment area;

the results of the efficacy calculations are shown in table 6:

TABLE 6 field efficacy test results of pyriproxyfen, monosultap and compound preparations on scale insects of citrus

As can be seen from the data in Table 6, the composition of pyriproxyfen and monosultap in different proportions has a significantly better prevention effect on citrus scale insects than the two single agents of pyriproxyfen and monosultap, and the three formulas have synergistic effects. The control effect of each compound preparation on the 14 th day after application is higher than that of the 7 th day, the control effect of each compound preparation on the 30 th day is lower than that of the 14 th day, and the control effect of each compound preparation is obviously improved compared with that of pyriproxyfen and monosultap.

The field efficacy test of the preparations of different formulations prepared in examples 1 to 4 for controlling bradysia odoriphaga was conducted to investigate the actual control effect on soil insects.

The field efficacy test site for the bradysia odoriphaga is south Chang city, Jiangxi province, ShihThe time interval is 2019, 8 and 27. The application method is root irrigation, and comprises setting 7 treatments, each of which is repeated 4 times, and has a cell area of 30m²。

The investigation method comprises the following steps: and (4) investigating the plant damage rate, sampling five Z-shaped points in each cell, taking 1 m-row Chinese chives at each point, and counting the number of damaged plants at 21d and 35d after application.

The calculation method comprises the following steps: calculating the reduction rate of insect population and correcting control effect of each treatment. The calculation formula is as follows:

percent reduction rate (%) of population (number of pre-pest control-number of post-pest control)/number of pre-pest control × 100

Correction control effect (%) - (oral cavity decline rate in drug treatment area-oral cavity decline rate in control area)/(oral cavity decline rate in control area) x 100

The test results are shown in table 7:

TABLE 7 field control test results of pyriproxyfen, monosultap and their mixed preparations against bradysia odoriphaga

As can be seen from the data in Table 7, the efficacy and duration of the pyriproxyfen and monosultap are optimized after being compounded. The pyriproxyfen single agent has a good effect of preventing and treating bradysia odoriphaga, and compared with pyriproxyfen, the monosultap single agent has a poor effect of preventing and treating bradysia odoriphaga.

The field efficacy test for preventing and controlling the citrus leaf miner is carried out on the preparations with different formulations prepared in examples 1-5, so as to investigate the actual control effect of the preparations on the citrus leaf miner. The test method comprises the following steps: refer to a related test method in GB/T17980.58-2004 'pesticide field efficacy experiment criterion (I) insecticide control citrus leaf miner'. The test results are shown in table 8:

table 8 results of field control test of pyriproxyfen, monosultap and mixed preparation thereof on citrus leaf miner

As can be seen from the data in Table 8, the control effect of the single pesticide on the citrus leaf miner is poor, and the control effect of the single pyriproxyfen on the citrus leaf miner is superior to that of the single pesticide, which is related to the difference of the action mechanisms of the two pesticides. The pyriproxyfen and monosultap are compounded in different proportions, the control effect on citrus leaf miner is superior to the single-agent control effect of the two active ingredients, and the persistence is excellent.

The formulations of different formulations prepared in examples 1-3 were subjected to a field efficacy test for controlling liriomyza sativae on beans to examine the actual control effect thereof on liriomyza sativae on beans.

The field efficacy test site of the liriomyza sativae on kidney beans is in the Songjiang area of Shanghai city, and the time is 9 months and 12 days in 2019.

The test crop is kidney bean, and the test plot is 18m²Repeating the treatment for 4 times, additionally arranging blank control, randomly combining and arranging all the cells, and uniformly cultivating the cells.

The application method is spraying.

The population number was investigated before application, and 3d, 5d, 10d, 15d, and 20d after application. During the investigation, 10 beans are selected in the middle of each cell, 2 leaves are selected on the middle and upper parts of each bean for investigation, the number of live insects is recorded, the control effect is calculated, and the test results are shown in table 9.

The corrected control effect (%) (oral cavity reduction rate in the drug-treated area-oral cavity reduction rate in the control area)/(oral cavity reduction rate in the control area) x 100. The test results are shown in table 9:

TABLE 9 field control test results of pyriproxyfen and monosultap and their mixed preparations on liriomyza sativae on kidney bean

As can be seen from the data in Table 9, the pyriproxyfen and monosultap have poor control effect on liriomyza sativae, and the control effect of the pyriproxyfen and monosultap compounded in different proportions on the liriomyza sativae is superior to the control effect of the two active ingredients on the single agents, and the persistence is excellent.

In conclusion, the data in tables 5 to 9 show that the compounding of monosultap and pyriproxyfen expands the insecticidal control spectrum, remarkably reduces the dosage of the pesticide, prolongs the effective period, reduces the pesticide application times, and reduces the pesticide cost and environmental stress of farmers.

While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention.

Claims

1. The insecticidal composition containing pyriproxyfen and monosultap is characterized by comprising pyriproxyfen and monosultap, wherein the mass ratio of the pyriproxyfen to the monosultap is 1: 1-60.

2. The insecticidal composition containing pyriproxyfen and monosultap as claimed in claim 1, wherein the mass ratio of pyriproxyfen to monosultap is 1: 2-50.

3. The insecticidal composition containing pyriproxyfen and monosultap as claimed in claim 2, wherein the mass ratio of pyriproxyfen to monosultap is 1: 5-40.

4. The insecticidal composition containing pyriproxyfen and monosultap as claimed in claim 3, wherein the mass ratio of pyriproxyfen to monosultap is 1: 9-30.

5. The insecticidal composition containing pyriproxyfen and monosultap as claimed in claim 4, wherein the mass ratio of pyriproxyfen to monosultap is 1: 10-20.

6. The insecticidal composition containing pyriproxyfen and monosultap as claimed in claim 1, wherein the mass sum of pyriproxyfen and monosultap accounts for 1-99% of the total mass of the insecticidal composition.

7. The insecticidal composition containing pyriproxyfen and monosultap as claimed in claim 6, wherein the mass sum of pyriproxyfen and monosultap accounts for 5-95% of the total mass of the insecticidal composition.

8. The insecticidal composition containing pyriproxyfen and monosultap as claimed in claim 7, wherein the mass sum of pyriproxyfen and monosultap accounts for 10-80% of the total mass of the insecticidal composition.

9. The insecticidal composition containing pyriproxyfen and monosultap as claimed in claim 1, wherein the formulation of the insecticidal composition includes but is not limited to nano-microemulsion, soluble liquid agent, soluble powder agent, wettable powder agent, soluble granule agent, aqueous emulsion, aqueous suspension, emulsifiable concentrate, and emulsion powder agent.

10. Use of a pesticidal composition containing pyriproxyfen and monosultap according to any one of claims 1 to 9 for controlling crop pests, which are lepidopteran pests, homopteran pests or subterranean pests.