CN112017132A - Vein image enhancement method based on maximum curvature method and multi-scale Hessian matrix - Google Patents
Vein image enhancement method based on maximum curvature method and multi-scale Hessian matrix Download PDFInfo
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Abstract
The invention discloses a vein image enhancement method based on a maximum curvature method and a multi-scale Hessian matrix, and belongs to the technical field of acquisition and processing of superficial vein distribution images. Firstly, determining vein central lines in four directions, setting the range and the iteration step length of a scale factor, then initializing the scale factor, then performing Hessian enhancement to obtain an enhanced image, and then iterating the scale factor until the scale factor exceeds the maximum value of the range; then, the enhancement results in all directions are compared, and the maximum value is reserved; and finally, adding the maximum correspondences in the four directions to obtain a final enhancement result. The vein image enhancement method based on the maximum curvature method and the multi-scale Hessian matrix has the advantages that the extracted vein structure is complete and coherent, the details are rich, and the condition that the enhancement effect of broken lines and weak veins in the single-scale method enhancement result is not obvious is improved.
Description
Technical Field
The invention relates to a vein image enhancement method based on a maximum curvature method and a multi-scale Hessian matrix, and belongs to the technical field of acquisition and processing of superficial vein distribution images.
Background
Acquisition and processing of superficial vein distribution images are one of important research subjects in the field of biomedical imaging, and have important application values in the fields of medical diagnosis and treatment, biological feature identification and the like. For example, in the field of clinical medicine, the feature extraction of superficial veins is helpful to improve the success rate of venipuncture, assist diagnosis and treatment of vascular diseases, and the like; the superficial veins are characterized by universality and uniqueness, are difficult to forge or alter, and are more user-friendly and safe for biometric identification as a living feature. However, due to the difference of factors such as skin pigment, the thickness of blood vessels, the positions and depths of blood vessels, the thickness of fat and the like, the vein conditions are different among different individuals, the vein detection capability is different, and particularly, the vein detection of special people such as infants, old people and obese patients is more difficult.
Due to different absorption capacities of biological tissues to infrared light, the strong absorption capacity of hemoglobin in vein blood vessels to infrared light enables a vein image which is clearer than the vein distribution situation of visible light imaging to be obtained through near infrared imaging. However, near infrared imaging has the defects of low image contrast, low signal-to-noise ratio, blurred vein texture edge, poor visual effect and the like, and is not enough to be directly used for imaging or auxiliary diagnosis and treatment and the like. In order to improve the definition of the vein image and suppress noise, so as to facilitate the identification of human eyes or machines, the acquired vein image needs to be enhanced.
Therefore, a vein image enhancement method based on the maximum curvature method and the multi-scale Hessian matrix is needed.
Disclosure of Invention
In order to solve the technical problems, the invention discloses a vein image enhancement method based on a maximum curvature method and a multi-scale Hessian matrix, which has the following specific technical scheme: comprises that
Step 4, pairRespectively carrying out Hessian enhancement to obtain enhanced images in four directions;
step 6, comparing the enhancement result graphs in all directions, and keeping the maximum value to obtain
And 7, adding the maximum responses in the four directions to obtain a final enhancement result.
To obtain a more accurate center point position, multiple directions of the vein image are detected, and representative angles of 0 °, 45 °, 90 ° and 135 ° are selected, as shown in fig. 1. And respectively carrying out curvature calculation on all cross section curves in the 4 directions of the vein image to obtain points of all local curvature maximum values, and forming a vein central point position detection result graph in the 4 directions.
By usingRepresenting pixel points on a vein imageIs determined by the gray-scale value of (a),then the image is representedA certain positionThe cross section curve of the upper part has a mapping functionSo that。
cross-sectional curve of vein imageIs concave upwards, then its curvatureThe value is positive and the lowest point of the fovea is the center point of the vein. One curve is recorded withEach concave region can be calculated to obtain a local curvature maximum value point, namely a vein central point, and the position of the vein central point is recorded asWherein, in the step (A),. The width of the concave region can be regarded as the width of the vein and is set as the center point of the veinWithin the neighborhood range of (2), the number of continuous pixels with positive curvature is recorded as。The larger the center pointThe greater the width of the vein in which it is located. To indicate the detected centre pointThe probability of the vein region located at each central point is provided with a scoreDefinition of. Projecting a score toPlane, as shown in the following formula:
Respectively carrying out the above calculation on cross section curves of the vein image in four directions of horizontal, vertical, 135 degrees and 45 degrees to obtain the preliminarily detected vein centerline images in the four directionsAs shown in fig. 2.
Further, in order to obtain a clear and accurate vein structure and suppress noise, a Hessian matrix is adopted to enhance the vein center images in four directions extracted by the maximum curvature method in the last step. The vein vessel is a linear tubular structure, and the vessel enhancement can be regarded as a filtering process for finding a linear structure. The Hessian matrix is sensitive to the structure, is often used for extracting linear structure features, and can be applied to two-dimensional and three-dimensional vein images.
The basic idea of the Hessian matrix is to extract the main direction of the local second order structural decomposition of the image. The eigenvalue and eigenvector of the Hessian matrix can be used to describe the local structure of the image, the eigenvector corresponding to the eigenvalue with the smallest absolute value represents the direction with the smallest curvature, i.e. along the eigen direction of the image, and the eigenvector corresponding to the eigenvalue with the largest absolute value represents the direction with the largest curvature, i.e. perpendicular to the eigen direction of the image.
A binary Hessian matrix is defined as follows:
wherein the content of the first and second substances,as an imageIn thatThe second partial derivative in the direction of the direction,is composed ofIn thatThe second partial derivative in the direction of the direction,representative imageIn thatDirection andthe mixed partial derivative in direction is shown as follows:
since the second-order partial derivative is sensitive to noise, the Hessian matrix is first subjected to gaussian smoothing, which is considered as the convolution of the image with the second-order gaussian derivative, as shown in the following formula:
wherein, in orderTwo-dimensional Gaussian function of scaleAnd the second order partial derivative thereof is shown as follows:
for vein central line images detected in four directionsHessian matrix enhancement is performed respectively. In the horizontal direction thereofFor example, willRespectively carrying out convolution operation with three second-order partial derivatives of the Gaussian function to obtain second-order partial derivatives at three different angles in horizontal, vertical and diagonal directions. For Hessian matrixThe absolute value of the characteristic value is searched and sequenced to obtain the characteristic valueAndfrom the feature values, a vein similarity function can be constructed.The above pixel points pass through the vein similarity function to obtain response output。
Further, in general, pixels in an image can be classified into three categories:
1. background, the gray distribution is more uniform, and the curvature in each direction is smaller;
2. isolated points, with greater curvature in each direction;
3. the blood vessel has a large curvature in the radial direction and a small curvature in the axial direction.
Table 1 summarizes the correspondence between eigenvalues of the Hessian matrix and structural shapes in the two-dimensional case,andrespectively representing the magnitude and magnitude of the characteristic value, the signs + and-then representing the positive and negative of the characteristic value, setting。
TABLE 1 relationship table of characteristic values of Hessian matrix of various possible shape structures under two-dimensional conditions
Further, the vein similarity function is defined as follows:
wherein the content of the first and second substances,can be used to distinguish structural shapes.The sensitivity for regulating and distinguishing block region and strip region, c has influence on the smooth degree of filtered image, S is a second-order structure, and has。Andset to 0.5 and 450, respectively.
Vein center line image for other directionsRespectively performing the same operation to obtain. The Hessian matrix enhancement results are shown in fig. 3.
Further, the maximum responses in the four directions are added to obtain the final enhancement result.
Vein central line image of four directions enhanced by Hessian matrixAndit is necessary to merge and filter out noise to achieve the final vein structure extraction. Every pixel point on every vein center imageComparing with two adjacent pixels on the left and right sides of the pixel, and connecting the pixels if the values of the pixel and the left and right pixels are larger; if the pixel point value is smaller, the two side pixelsIf the value of the point is larger, only marking the pixel point; if the pixel point is larger and the values of the pixel points on the two sides are smaller, the point is marked as noise. This process can be represented by the following formula:
the same operation is done in the other three directions as shown in the following equation:
finally, the four directional images are compared point by pointAndand taking the maximum value of the values of the same pixel point as a final vein feature image, wherein the maximum value is shown as the following formula:。
has the advantages that: the vein image enhancement method based on the maximum curvature method and the multi-scale Hessian matrix has the advantages that the extracted vein structure is complete and coherent, the details are rich, and the condition that the enhancement effect of broken lines and weak veins in the single-scale method enhancement result is not obvious is improved.
Drawings
FIG. 1 is a flow chart of the present invention;
FIG. 2 is a schematic diagram of the direction of curvature calculation of a cross-sectional curve;
figure 3 vein image vein centerline image in four directions: (a) original drawing; (b) level of(ii) a (c) Is perpendicular to ; (d) 135°;(e) 45°;
FIG. 4 is a diagram of a Hessian enhancement result of a vein central line image in four directions of a vein image; (a) original drawing; (b) level of(ii) a (c) Is perpendicular to; (d) 135°;(e) 45°;
FIG. 5 EMC and EMCs enhancement results compare: (a) original drawing; (b) EMC enhancement results; (c) (iii) EMCs enhancement results; (d) filtering burrs by Gaussian filtering after EMCs are enhanced;
FIG. 6 contrast results of different algorithm enhancements under the same lighting conditions: (a) original drawing; (b) CLAHE; (c) retinex; (d) EMC; (e) EMC-C; (f) EMC-fast; (g) EMCs;
FIG. 7 contrast the enhancement effect of different algorithms under different lighting conditions: (a) original drawing; (b) CLAHE; (c) retinex; (d) EMC; (e) EMC-fast; (f) EMCs.
Detailed Description
The present invention is further illustrated by the following figures and specific examples, which are to be understood as illustrative only and not as limiting the scope of the invention, which is to be given the full breadth of the appended claims and any and all equivalent modifications thereof which may occur to those skilled in the art upon reading the present specification.
The vein Enhancement Method (EMC) combining the maximum curvature method and the single-scale Hessian matrix enhancement can well extract main vein structures, but thin and weak deep veins are lost. To obtain more details of the vein, this section proposes to fuse Hessian enhancement features of different scales of the vein centerline.
The vein diameters in the vein images are different, and the vein structure extracted by adopting single-scale Hessian enhancement only has a part of veins with relatively consistent diameters, as shown in FIG. 4, the detected main veins are relatively obvious and thick veins on the original vein image. Some of the deeper and weaker veins are not enhanced or even completely unextracted. For better enhancement results, different size scales are setMatching veins of different diameters. Determining responses at different scalesThen, the maximum response is obtained as shown in the following formula:
the specific process is as follows:
4. To pairRespectively carrying out Hessian enhancement to obtain enhanced images in four directions;
6. then comparing the enhancement result graphs in all directions, and keeping the maximum value to obtainAnd;
7. and adding the maximum responses in the four directions to obtain a final enhancement result.
Compared with the EMC method, the enhancement result of the vein Enhancement Method (EMCs) combining the maximum curvature method and the multi-scale Hessian matrix enhancement is shown in FIG. 5, the contrast of the enhancement effect of the EMCs is higher, the vein structure is more consistent, and the broken line part in the EMC algorithm enhancement result is enhanced in the EMCs algorithm. The weak veins which are not obvious in the original vein image have better enhancement effect in the EMCs algorithm.
Results and analysis of the experiments
The invention improves a vein Enhancement Method (EMC) combining a maximum curvature method and Hessian matrix enhancement in the prior art, and provides a CUDA acceleration algorithm (EMC-C) of the EMC, a structure optimization algorithm (EMC-fast) based on the EMC-C and an EMCs algorithm of multi-scale Hessian matrix enhancement.
(1) Different algorithms enhance result comparison
The vein enhancement results of the different algorithms are shown for example in fig. 6 and 7:
as can be seen from fig. 6, the CLAHE algorithm highlights the vein structure to some extent, improving the image contrast, but at the same time the noise is also enhanced. The Retinex algorithm results in an overall gray value improvement, but does not enhance the venous structures well. Most veins are extracted by EMC and EMC-C algorithms, the contrast of an EMC-fast enhancement result is higher, but the three algorithms have weaker enhancement capability on the thin and weak veins, and the extracted vein structure has a fracture condition. The EMCs algorithm provided for realizing more accurate vein image enhancement further improves the enhancement effect, has higher image contrast, more complete vein structure, few broken lines and richer details, and can highlight weak veins with unobvious enhancement effect in other methods.
Fig. 7 shows the enhancement effect of each algorithm under different lighting conditions. Therefore, most algorithms including CLAHE, EMC and EMC-fast have poor enhancement effect on the vein images acquired under the dark light condition, and the Retinex algorithm has better enhancement effect on the images under the dark light than under the bright light condition, but enhances the noise. The enhancement effect of the EMCs algorithm is not influenced by the illumination condition, and images with high contrast and rich details can be enhanced.
(2) Enhanced image quality assessment
Since the vein image lacks a corresponding clear reference image, several commonly used non-reference image quality evaluation functions are used to analyze the enhancement effect.
(2.1) gradient function Brenner
Brenner is a simple gradient evaluation function, which is the sum of the squares of the differences in gray values of any two adjacent pixels on the image, as shown by the following equation:
(2.2) Gray-level differential product function SMD2
The gray difference product function takes the gray change as an evaluation criterion, and accumulates the product of the gray difference between each pixel and two adjacent pixels, as shown in the following formula:
(2.3) standard deviation STD
The standard deviation describes the degree of dispersion of the pixel gray scale value from the mean value on the image, and the larger the standard deviation, the more dispersed the image gray scale is, the better the image quality is, as shown in the following formula:
wherein the content of the first and second substances,is the size of the image size and,is the image mean.
The enhancement performance indicators for the different algorithms are shown in table 2.
TABLE 2 different algorithms enhancement effect index
As can be seen from Table 2, the enhancement results of the method (EMCs) combining the maximum curvature method and the multi-scale Hessian matrix and the EMC method (EMC-fast) subjected to CUDA acceleration and structure optimization are superior to other methods in three indexes of Brenner, SMD2 and STD, and are greatly improved relative to the original vein image. Among them, the indexes of EMCs are the best. Compared with EMC, EMC-fast and EMCs are both more advantageous in vein image enhancement, and the enhancement result indexes are about 1.5 times and 1.95 times of EMC respectively.
In summary, in order to enhance more vein structure details and realize the extraction of fine and deep veins, the invention proposes to combine a multiscale Hessian matrix with a maximum curvature method on the basis of the original algorithm and set the scaleDifferent Hessian matrixes are matched and enhanced with vein vessels with different diameters, and then multi-scale enhancement results are fused. Experiments prove that compared with other traditional image enhancement methods and the original vein image enhancement algorithm based on the maximum curvature method and the Hessian matrix, the maximum curvature method and the multi-scale Hessian combined method can obtain the enhancement effect with higher contrast, the extracted vein structure is complete and coherent, the details are rich, and the condition that the enhancement effect of broken lines and weak veins in the single-scale method enhancement result is not obvious is improved.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (6)
1. A vein image enhancement method based on a maximum curvature method and a multi-scale Hessian matrix is characterized in that: comprises that
Step 4, pairRespectively carrying out Hessian enhancement to obtain enhanced images in four directions
step 6, comparing the enhancement result graphs in all directions, and keeping the maximum value to obtain
And 7, adding the maximum responses in the four directions to obtain a final enhancement result.
2. The vein image enhancement method based on the maximum curvature method and the multi-scale Hessian matrix according to claim 1, wherein: vein center line in the step 1The obtaining step comprises:
step 1.1: detecting a plurality of directions of the vein image, and selecting representative angles of 0 degree, 45 degrees, 90 degrees and 135 degrees;
step 1.2: respectively carrying out curvature calculation on all cross section curves in the 4 directions of the vein image to obtain points of all local curvature maximum values, and forming a vein central point position detection result graph in the 4 directions;
step 1.3: by usingRepresenting pixel points on a vein imageIs determined by the gray-scale value of (a),then the image is representedA certain positionThe cross section curve of the upper part has a mapping functionSo that(ii) a CurvatureThe formula of (c) is shown as follows:
step 1.4: cross-sectional curve of vein imageIs concave upwards, then its curvatureThe value is positive, and the lowest point of the concave area is the central point of the vein;one curve is recorded withEach concave region can be calculated to obtain a local curvature maximum value point, namely a vein central point, and the position of the vein central point is recorded asWherein, in the step (A),(ii) a The width of the concave region is the width of the vein and is set as the center point of the veinWithin the neighborhood range of (2), the number of continuous pixels with positive curvature is recorded as;The larger the center pointThe greater the width of the vein in which it is located; to indicate the detected centre pointThe probability of the vein region located at each central point is provided with a scoreDefinition of the fractionProjected to the targetPlane, as shown in the following formula:
3. The vein image enhancement method based on the maximum curvature method and the multi-scale Hessian matrix according to claim 1, wherein: the Hessian enhancement in the step 3 comprises the following steps:
step 3.1: will be provided withRespectively carrying out convolution operation with three second-order partial derivatives of the Gaussian function to obtain second-order partial derivatives at three different angles in horizontal, vertical and diagonal directions
Step 3.2: for Hessian matrixThe absolute value of the characteristic value is searched and sequenced to obtain the characteristic valueAndconstructing a vein similarity function from the characteristic values;
step 3.3:the above pixel points pass through the vein similarity function to obtain response output;
4. The vein image enhancement method based on the maximum curvature method and the multi-scale Hessian matrix according to claim 3, wherein: obtaining the characteristic value in the step 3.2Andcomparing the corresponding relation between the characteristic value of the Hessian matrix and the structural shape under the two-dimensional condition; definition H and L represent the magnitude and magnitude of the characteristic value, respectively, and the definition symbols + and-represent the positive and negative of the characteristic value, respectively(ii) a When the linear structure is bright, the characteristic value L corresponds toCharacteristic value H-corresponding(ii) a When the linear structure is dark, the characteristic value corresponds toThe characteristic value H + corresponds to(ii) a When the lumpy structure is bright, the characteristic value H-corresponds toCharacteristic value H-corresponding(ii) a When the lump-shaped structure is dark, the characteristic value H + corresponds toCharacteristic value H-corresponding。
5. The vein image enhancement method based on the maximum curvature method and the multi-scale Hessian matrix according to claim 4, wherein: the vein similarity function in step 3.2 is defined as follows:
wherein the content of the first and second substances,can be used to distinguish structural shapes;
6. The vein image enhancement method based on the maximum curvature method and the multi-scale Hessian matrix according to claim 1, wherein: the step of gaussian convolution in step 3.1 is to define a binary Hessian matrix as follows:
wherein the content of the first and second substances,as an imageIn thatThe second partial derivative of the direction is,is composed ofIn thatThe second partial derivative in the direction of the direction,representative imageIn thatDirection andthe mixed partial derivatives in direction, I (x, y), represent the two-dimensional image as shown in the following equation:
since the second partial derivative is sensitive to noise, when the Hessian matrix is calculated, gaussian smoothing, i.e. convolution of the image with the second derivative of gaussian, is performed as shown in the following equation:
wherein, in orderTwo-dimensional Gaussian function of scaleAnd the second order partial derivative thereof is shown as follows:
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