CN112011648B - Method for detecting heterophilic mouse leukemia virus in biological product - Google Patents
Method for detecting heterophilic mouse leukemia virus in biological product Download PDFInfo
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- CN112011648B CN112011648B CN202011176128.5A CN202011176128A CN112011648B CN 112011648 B CN112011648 B CN 112011648B CN 202011176128 A CN202011176128 A CN 202011176128A CN 112011648 B CN112011648 B CN 112011648B
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- 241000700605 Viruses Species 0.000 title claims abstract description 32
- 208000032839 leukemia Diseases 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 23
- 239000000523 sample Substances 0.000 claims abstract description 52
- 238000006243 chemical reaction Methods 0.000 claims abstract description 22
- 238000011144 upstream manufacturing Methods 0.000 claims abstract description 13
- 238000003753 real-time PCR Methods 0.000 claims abstract description 12
- 239000000126 substance Substances 0.000 claims description 9
- 125000006853 reporter group Chemical group 0.000 claims description 5
- 238000010791 quenching Methods 0.000 claims description 4
- 230000000171 quenching effect Effects 0.000 claims description 4
- ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 claims description 3
- 238000001514 detection method Methods 0.000 abstract description 15
- 230000035945 sensitivity Effects 0.000 abstract description 9
- 238000011084 recovery Methods 0.000 abstract description 4
- 230000003321 amplification Effects 0.000 description 29
- 238000003199 nucleic acid amplification method Methods 0.000 description 29
- 230000000052 comparative effect Effects 0.000 description 25
- 108020004414 DNA Proteins 0.000 description 20
- 239000000047 product Substances 0.000 description 12
- 238000010586 diagram Methods 0.000 description 6
- 150000007523 nucleic acids Chemical group 0.000 description 6
- 239000006228 supernatant Substances 0.000 description 5
- 108020004707 nucleic acids Proteins 0.000 description 4
- 102000039446 nucleic acids Human genes 0.000 description 4
- 108700004025 env Genes Proteins 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 241000714177 Murine leukemia virus Species 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 238000000137 annealing Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- 101150030339 env gene Proteins 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 230000000415 inactivating effect Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000010839 reverse transcription Methods 0.000 description 2
- 238000003757 reverse transcription PCR Methods 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 241001430294 unidentified retrovirus Species 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000725303 Human immunodeficiency virus Species 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 108020005202 Viral DNA Proteins 0.000 description 1
- 108020000999 Viral RNA Proteins 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
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- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000001566 pro-viral effect Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/702—Specific hybridization probes for retroviruses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes
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- Organic Chemistry (AREA)
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- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Virology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
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Priority Applications (1)
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CN202011176128.5A CN112011648B (en) | 2020-10-29 | 2020-10-29 | Method for detecting heterophilic mouse leukemia virus in biological product |
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CN202011176128.5A CN112011648B (en) | 2020-10-29 | 2020-10-29 | Method for detecting heterophilic mouse leukemia virus in biological product |
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CN112011648A CN112011648A (en) | 2020-12-01 |
CN112011648B true CN112011648B (en) | 2021-01-15 |
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CN202011176128.5A Active CN112011648B (en) | 2020-10-29 | 2020-10-29 | Method for detecting heterophilic mouse leukemia virus in biological product |
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Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN114317821B (en) * | 2021-12-27 | 2023-11-24 | 苏州良辰生物医药科技有限公司 | Method for rapidly detecting leukemia virus of rat |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108531662A (en) * | 2018-07-02 | 2018-09-14 | 苏州良辰生物医药科技有限公司 | A kind of specific primer and detection method of different preferendum mouse leukemia virus |
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2020
- 2020-10-29 CN CN202011176128.5A patent/CN112011648B/en active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108531662A (en) * | 2018-07-02 | 2018-09-14 | 苏州良辰生物医药科技有限公司 | A kind of specific primer and detection method of different preferendum mouse leukemia virus |
Non-Patent Citations (1)
Title |
---|
Ning Tang.Development of sensitive single-round pol or env RT-PCR assays to screen for XMRV in multiple sample types.《Journal of Virological Methods》.2011,第179卷第127-134页, 尤其是第128-133页,材料与方法和结果和讨论和图3. * |
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Application publication date: 20201201 Assignee: Jiangsu Jinmao Finance Leasing Co.,Ltd. Assignor: LIANGCHEN BIO (SUZHOU) Corp. Contract record no.: X2023320010004 Denomination of invention: A Method for Detecting Heterophilic Mouse Leukemia Virus in Biological Products Granted publication date: 20210115 License type: Exclusive License Record date: 20230106 |
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PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
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Denomination of invention: A Method for Detecting Heterophilic Mouse Leukemia Virus in Biological Products Effective date of registration: 20230109 Granted publication date: 20210115 Pledgee: Jiangsu Jinmao Finance Leasing Co.,Ltd. Pledgor: LIANGCHEN BIO (SUZHOU) Corp. Registration number: Y2023320010019 |
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EC01 | Cancellation of recordation of patent licensing contract | ||
EC01 | Cancellation of recordation of patent licensing contract |
Assignee: Jiangsu Jinmao Finance Leasing Co.,Ltd. Assignor: LIANGCHEN BIO (SUZHOU) Corp. Contract record no.: X2023320010004 Date of cancellation: 20240203 |
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PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Granted publication date: 20210115 Pledgee: Jiangsu Jinmao Finance Leasing Co.,Ltd. Pledgor: LIANGCHEN BIO (SUZHOU) Corp. Registration number: Y2023320010019 |