CN112011460A - Biological cell culture device based on electromagnetic damping principle - Google Patents

Biological cell culture device based on electromagnetic damping principle Download PDF

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Publication number
CN112011460A
CN112011460A CN202010822063.0A CN202010822063A CN112011460A CN 112011460 A CN112011460 A CN 112011460A CN 202010822063 A CN202010822063 A CN 202010822063A CN 112011460 A CN112011460 A CN 112011460A
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magnetic
cell culture
incubator
biological cell
electromagnetic damping
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陈梓杰
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/26Conditioning fluids entering or exiting the reaction vessel
    • FMECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16FSPRINGS; SHOCK-ABSORBERS; MEANS FOR DAMPING VIBRATION
    • F16F15/00Suppression of vibrations in systems; Means or arrangements for avoiding or reducing out-of-balance forces, e.g. due to motion
    • F16F15/02Suppression of vibrations of non-rotating, e.g. reciprocating systems; Suppression of vibrations of rotating systems by use of members not moving with the rotating systems
    • F16F15/03Suppression of vibrations of non-rotating, e.g. reciprocating systems; Suppression of vibrations of rotating systems by use of members not moving with the rotating systems using magnetic or electromagnetic means

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
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  • Life Sciences & Earth Sciences (AREA)
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  • Chemical & Material Sciences (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
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  • Biochemistry (AREA)
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Abstract

The invention relates to the technical field of biological cell culture equipment, and discloses a biological cell culture device based on an electromagnetic damping principle. This biological cell culture device based on electromagnetic damping principle drives the dwang through the backup pad and rotates, and the dwang passes through a screw section of thick bamboo and rotates, and the dwang drives the pendulum rod and rotates, and the pendulum rod drives the liquid feeding pipe and moves the middle part of culture dish and carry out the interpolation of nutrient solution, and at the liquid feeding pipe shutoff in the incubator, consequently can only carry out the interpolation that has the nutrient solution in the household utensils that have the culture dish. This structure avoids the problem that the nutrient solution is wasted and the culture dish needs to be added one by one.

Description

Biological cell culture device based on electromagnetic damping principle
Technical Field
The invention relates to the technical field of biological cell culture equipment, in particular to a biological cell culture device based on an electromagnetic damping principle.
Background
The cell culture technology can be used for culturing a single cell into simple single cells or cells with few differentiation in a large scale, which is an indispensable link of the cloning technology, and the cell culture is the cloning of the cell. Biological cell culture technology is an important and common technology in cell biology research methods, and a large number of cells can be obtained through cell culture, and signal transduction, anabolism, growth and proliferation of the cells and the like of the cells can be researched through the biological cell culture technology.
When the existing cell culture box is taken out and placed in a culture dish, the cell culture box can be placed only in a single layer, and nutrient solution is conveniently added into the single layer of the culture dish; to the culture apparatus of multilayer setting, when placing, can not lead to placing inconvenient and place the great problem of in-process culture dish slip because the interlayer is too dark, and when it takes out, need take out the culture dish of upside in advance, this not only the operation degree of difficulty is big and make other culture dishes expose the time overlength outside the box, we provide a biological cell culture apparatus based on electromagnetic damping principle in order to solve this problem.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a biological cell culture device based on an electromagnetic damping principle, which has the advantages of large storage space, high stability and convenience in adding and taking nutrient solution, and solves the problems that the existing biological cell culture device has a single-layer storage space, is poor in stability, is inconvenient for adding the nutrient solution and is cumbersome in taking.
(II) technical scheme
In order to realize the purposes of large storage space, high stability and convenience for adding nutrient solution and taking, the invention provides the following technical scheme: a biological cell culture device based on the electromagnetic damping principle comprises an incubator, wherein rotary columns are arranged inside the incubator, a sliding groove is formed in the middle of the incubator, a metal layer is arranged inside the incubator and in the sliding groove, grooves are formed in the left end and the right end of the metal layer, a magnetic ring is connected inside the metal layer in a sliding mode, an inclined plane column corresponding to the groove is arranged on the outer side of the magnetic ring, a magnetic isolating layer is fixedly connected inside the magnetic ring, a culture dish is fixedly connected inside the magnetic isolating layer, a support plate is rotatably connected inside the incubator and outside the rotary columns, a magnetic handle is arranged at the non-support end of the support plate, a spring is fixedly connected between the lower portion of the magnetic handle and the incubator, a rotary rod is connected to the upper end of the support plate in a sliding mode, an arc-shaped ball is arranged at the lower end of the rotary rod, the swing rod is provided with a liquid feeding pipe at one end far away from the rotating rod, and a threaded cylinder corresponding to the rotating rod is arranged in the incubator.
Preferably, the inclined plane column is inclined and corresponds to the groove and the sliding groove, so that the magnetic isolation layer and the culture dish are guaranteed to move up and down stably through the magnetic ring.
Preferably, the metal level is closed annular, and is located the outside of magnetic ring to through the corresponding speed that slows down culture dish reciprocating motion of electromagnetic damping, thereby avoid the nutrient solution and the biological cell of culture dish to spill.
Preferably, the magnetic handle corresponds to the magnetic pole of the magnetic disk on the opposite surface, and a part of the magnetic disk is provided with a magnet, so that the magnetic disk after rotation pushes the magnetic handle to swing, and the magnetic handle drives the support plate to extend out through the rotary column.
Preferably, the spring constant of the lowermost spring is smaller than the spring constant of the upper spring, thereby ensuring that the lowermost spring pushes the support plate to extend.
Preferably, the inside of the incubator is provided with an arc-shaped groove corresponding to the swing rod, so that the swing rod can be conveniently retracted.
Preferably, the middle part of dwang is provided with the screw thread, and corresponding with a screw thread section of thick bamboo to through culture dish extrusion backup pad, the backup pad passes through the rotary column and drives the magnetic handle rotation, and the magnetic handle promotes the dwang upwards. The rotating rod rotates through the threaded cylinder and drives the magnetic disk to rotate, and the magnetic disk pushes the supporting plate on the upper side to swing through the magnet on the magnetic disk, so that the supporting plate is moved upwards to extend out.
(III) advantageous effects
Compared with the prior art, the invention provides a biological cell culture device based on the electromagnetic damping principle, which has the following beneficial effects:
1. this biological cell culture apparatus based on electromagnetic damping principle through placing the culture dish in the magnetism isolating layer, culture dish and magnetism isolating layer pass through the inclined plane post on the magnetic ring and stably move in the recess of metal level, because the magnetic ring is the magnet, and the metal level is closed the annular to through the corresponding speed that slows down the culture dish and reciprocate the removal of electromagnetic damping, thereby avoid the nutrient solution and the biological cell of culture dish to spill.
2. This biological cell culture device based on electromagnetic damping principle through squeezing the backup pad through the culture dish, and the backup pad passes through the column rotation and drives the rotation of magnetic handle, and the magnetic handle promotes the dwang upwards. The dwang passes through a screw section of thick bamboo and rotates, and drives the disk and rotate, and the disk promotes the backup pad swing of upside through magnet above that to reach the stretching out of shifting up the backup pad, further reach the linkage that the backup pad was put in proper order and stretch out, and current biological cell culture device storage space has been solved to this structure is mostly the individual layer, is not convenient for add the nutrient solution and gets and take loaded down with trivial details problem.
3. This biological cell culture device based on electromagnetic damping principle drives the dwang through the backup pad and rotates, and the dwang passes through a screw section of thick bamboo and rotates, and the dwang drives the pendulum rod and rotates, and the pendulum rod drives the liquid feeding pipe and moves the middle part of culture dish and carry out the interpolation of nutrient solution, and at the liquid feeding pipe shutoff in the incubator, consequently can only carry out the interpolation that has the nutrient solution in the household utensils that have the culture dish. This structure avoids the problem that the nutrient solution is wasted and the culture dish needs to be added one by one.
Drawings
FIG. 1 is a schematic top view of the present invention;
FIG. 2 is an enlarged view of the structure of FIG. 1 at B according to the present invention;
FIG. 3 is a cross-sectional view of the structure of FIG. 1 taken along line A-A thereof in accordance with the present invention;
FIG. 4 is an enlarged view of the structure of FIG. 3 at C according to the present invention;
FIG. 5 is a partial structural view of the support plate of the present invention in an initial state;
fig. 6 is a sectional view of the magnetic ring according to the present invention.
In the figure: 1 incubator, 101 rotating columns, 102 sliding chutes, 2 metal layers, 201 grooves, 3 magnetic rings, 301 inclined plane columns, 4 magnetic isolating layers, 5 culture dishes, 6 supporting plates, 601 magnetic handles, 7 springs, 8 rotating rods, 801 arc balls, 9 magnetic disks, 10 oscillating bars, 11 liquid feeding pipes and 12 threaded cylinders.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-6, a biological cell culture device based on electromagnetic damping principle comprises an incubator 1, wherein rotary columns 101 are arranged inside the incubator 1, a sliding groove 102 is formed in the middle of the incubator 1, a metal layer 2 is arranged inside the incubator 1 and in the sliding groove 102, grooves 201 are formed in the left end and the right end of the metal layer 2, a magnetic ring 3 is connected inside the metal layer 2 in a sliding mode, an inclined column 301 corresponding to the groove 201 is arranged on the outer side of the magnetic ring 3, a magnetism isolating layer 4 is fixedly connected inside the magnetic ring 3, a culture dish 5 is fixedly connected inside the magnetism isolating layer 4, the inclined column 301 is inclined and corresponds to the groove 201 and the sliding groove 102, and therefore the up-down movement stability of the magnetism isolating layer 4 and the culture dish 5 is guaranteed through the magnetic ring 3. The metal level 2 is for sealing the annular, and is located the outside of magnetic ring 3 to through the corresponding speed that slows down culture dish 5 and reciprocate the removal of electromagnetic damping, thereby avoid culture dish 5's nutrient solution and biological cell to spill.
The inside of incubator 1 just rotates in the outside of rotary column 101 and is connected with backup pad 6, and the unsupported end of backup pad 6 is provided with magnetic handle 6, fixedly connected with spring 7 between the lower part of magnetic handle 6 and incubator 1, and the elastic coefficient of downside spring 7 is less than the elastic coefficient of spring 7 on upper portion to guarantee that downside spring 7 promotes backup pad 6 and stretches out. The upper end sliding connection of backup pad 6 has dwang 8, and the lower extreme of dwang 8 is provided with arc ball 801, and the upper end fixedly connected with magnetic disc 9 of dwang 8, the magnetic pole of the magnetic disc 9 corresponding with the opposite face of magnetic stem 601 is corresponding, and a part of magnetic disc 9 is provided with the magnet to guarantee that magnetic disc 9 after the rotation promotes the swing of magnetic stem 601, magnetic stem 601 passes through rotary column 101 and drives backup pad 6 and stretch out. The middle part of the rotating rod 8 is fixedly connected with a swing rod 10, and an arc-shaped groove corresponding to the swing rod 10 is formed in the incubator 1, so that the swing rod 10 can be conveniently folded and unfolded. The one end of pendulum rod 10 keeping away from dwang 8 is provided with liquid feeding pipe 11, and the inside of incubator 1 is provided with the screw thread section of thick bamboo 12 that corresponds with dwang 8, and the middle part of dwang 8 is provided with the screw thread, and corresponds with screw thread section of thick bamboo 12 to through culture dish 5 extrusion backup pad 6, backup pad 6 drives magnetic handle 601 through rotary column 101 and rotates, and magnetic handle 601 promotes dwang 8 upwards. The rotating rod 8 rotates through the threaded cylinder 12 and drives the magnetic disk 9 to rotate, and the magnetic disk 9 pushes the upper supporting plate 6 to swing through the magnet on the magnetic disk, so that the support plate 6 is moved upwards to extend.
The working principle is as follows: this biological cell culture device based on electromagnetic damping principle, when using, through placing culture dish 5 in magnetism isolating layer 4, culture dish 5 and magnetism isolating layer 4 are through the stable removal of inclined plane post 301 on magnetic ring 3 in the recess 201 of metal level 2, because magnetic ring 3 is the magnet, and metal level 2 is closed ring shape to the corresponding speed that slows down culture dish 5 and reciprocate the removal through electromagnetic damping, thereby avoid culture dish 5's nutrient solution and biological cell to spill.
By pressing the support plate 6 through the culture dish 5, the support plate 6 drives the magnetic handle 601 to rotate through the rotating column 101, and the magnetic handle 601 pushes the rotating rod 8 upward. Dwang 8 rotates through a screw section of thick bamboo 12, and drives disk 9 and rotate, and disk 9 promotes the 6 swings of backup pad of upside through the magnet above that to reach the stretching out of shifting up backup pad 6, further reach the linkage that 6 pendulums of backup pad in proper order and stretch out, and current biological cell culture device storage space has been solved to this structure is mostly the individual layer, is not convenient for add the nutrient solution and gets and take loaded down with trivial details problem.
The rotating rod 8 is driven to rotate through the supporting plate 6, the rotating rod 8 rotates through the threaded cylinder 12, the rotating rod 8 drives the swing rod 10 to rotate, the swing rod 10 drives the liquid feeding pipe 11 to move to the middle of the culture dish 5 to carry out addition of nutrient solution, and the liquid feeding pipe 11 is plugged in the culture box 1, so that the nutrient solution can be added only in a vessel with the culture dish 5. This structure avoids the problems of the waste of the nutrient solution and the need to add the culture dishes 5 one by one.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (7)

1. The utility model provides a biological cell culture device based on electromagnetic damping principle, includes incubator (1), its characterized in that: the incubator is characterized in that rotary columns (101) are arranged inside the incubator (1), a sliding groove (102) is formed in the middle of the incubator (1), metal layers (2) are arranged inside the incubator (1) and in the sliding groove (102), grooves (201) are formed in the left end and the right end of each metal layer (2), magnetic rings (3) are connected inside the metal layers (2) in a sliding mode, inclined plane columns (301) corresponding to the grooves (201) are arranged on the outer sides of the magnetic rings (3), magnetic isolating layers (4) are fixedly connected inside the magnetic rings (3), culture dishes (5) are fixedly connected inside the magnetic isolating layers (4), a support plate (6) is rotatably connected inside the incubator (1) and outside the rotary columns (101), a magnetic handle (6) is arranged at the non-supporting end of the support plate (6), springs (7) are fixedly connected between the lower portions of the magnetic handles (6) and the incubator (1), and a top end of the rotary rod (6) is connected with a sliding, the lower end of the rotating rod (8) is provided with an arc-shaped ball (801), the upper end of the rotating rod (8) is fixedly connected with a magnetic disk (9), the middle of the rotating rod (8) is fixedly connected with a swinging rod (10), one end of the swinging rod (10) far away from the rotating rod (8) is provided with a liquid adding pipe (11), and the inside of the incubator (1) is provided with a threaded cylinder (12) corresponding to the rotating rod (8).
2. A biological cell culture apparatus based on electromagnetic damping principle according to claim 1, characterized in that: the inclined plane column (301) is inclined and corresponds to the groove (201) and the sliding groove (102).
3. A biological cell culture apparatus based on electromagnetic damping principle according to claim 1, characterized in that: the metal layer (2) is in a closed ring shape and is positioned on the outer side of the magnetic ring (3).
4. A biological cell culture apparatus based on electromagnetic damping principle according to claim 1, characterized in that: the magnetic handle (601) corresponds to the magnetic poles of the magnetic disk (9) on the opposite surface, and a part of the magnetic disk (9) is provided with a magnet.
5. A biological cell culture apparatus based on electromagnetic damping principle according to claim 1, characterized in that: the elastic coefficient of the lowermost spring (7) is smaller than the elastic coefficient of the upper spring (7).
6. A biological cell culture apparatus based on electromagnetic damping principle according to claim 1, characterized in that: an arc-shaped groove corresponding to the swing rod (10) is formed in the incubator (1).
7. A biological cell culture apparatus based on electromagnetic damping principle according to claim 1, characterized in that: the middle part of the rotating rod (8) is provided with threads and corresponds to the thread cylinder (12).
CN202010822063.0A 2020-08-15 2020-08-15 Biological cell culture device based on electromagnetic damping principle Withdrawn CN112011460A (en)

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CN202010822063.0A CN112011460A (en) 2020-08-15 2020-08-15 Biological cell culture device based on electromagnetic damping principle

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Application Number Priority Date Filing Date Title
CN202010822063.0A CN112011460A (en) 2020-08-15 2020-08-15 Biological cell culture device based on electromagnetic damping principle

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112608840A (en) * 2020-12-16 2021-04-06 李娄 Novel adjustable cell culture dish frame
CN114958547A (en) * 2022-06-29 2022-08-30 珠海美华医疗科技有限公司 Mycoplasma culture form viewing device

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112608840A (en) * 2020-12-16 2021-04-06 李娄 Novel adjustable cell culture dish frame
CN112608840B (en) * 2020-12-16 2023-08-25 上海倍锦生物科技有限公司 Novel adjustable cell culture dish frame
CN114958547A (en) * 2022-06-29 2022-08-30 珠海美华医疗科技有限公司 Mycoplasma culture form viewing device

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Application publication date: 20201201