CN112006919A

CN112006919A - Probiotics capsule wet tissue

Info

Publication number: CN112006919A
Application number: CN202010952830.XA
Authority: CN
Inventors: 韩郸; 赵丹妮
Original assignee: Shanghai Changsheng Biotechnology Co ltd
Current assignee: Shanghai Changsheng Biotechnology Co ltd
Priority date: 2020-09-11
Filing date: 2020-09-11
Publication date: 2020-12-01

Abstract

The application belongs to the field of biomedicine and discloses a probiotic capsule wet tissue, wherein a carrier layer coated with probiotics is arranged on a textile layer; the carrier layer coated with the probiotics contains a carrier coated with the probiotics; the carrier coated with probiotics is zeolite containing probiotics and high-molecular-weight polysaccharideOne or more of ridge soil, clay, bentonite and active carbon is fixed with the textile fabric layer by sticking, inlaying, adsorbing, hot pressing or sewing; drying wet towel and compressing to volume of no more than 6cm³. The wet tissue has good air permeability, can effectively promote the balance of skin flora, increase the immunity of the skin and reduce the occurrence of phenomena such as allergy on the basis of cleaning and disinfection.

Description

Probiotics capsule wet tissue

Technical Field

The application belongs to the field of biomedicine, and particularly relates to a wet tissue containing a loaded probiotic carrier. The application also comprises a preparation method and application of the wet tissue.

Background

Wet wipes, typically moist paper towels, are used to wipe the skin. Although the variety of the wet tissues is not as much as that of the paper tissues, the wet tissues become necessities of daily life along with the improvement of the awareness of cleanness and sanitation and the convenience of daily life. Especially for the old, the children, the sick and wounded or the disabled, the use frequency of the wet tissue is obviously increased.

At present, the types of domestic wet tissues are mostly limited to the types of common cleaning and antibacterial wet tissues, the types of the wet tissues in developed countries and regions in the world, particularly North America, Europe, Japan and the like, far exceed the cognitive range of people in China, and the physical surface disinfection wet tissues are popular abroad and just started in China. Domestic wet tissues can be divided into common wet tissues, sanitary wet tissues and disinfection wet tissues according to purposes. The common wet tissue is a product which takes non-woven fabric, dust-free paper or other raw materials as a carrier, takes purified water as production water, and is added with a proper amount of auxiliary materials such as preservative and the like to have a cleaning effect on hands, skin mucous membranes or object surfaces. The sanitary wet tissue is a product which takes non-woven fabric, dust-free paper or other raw materials as a carrier, takes purified water as production water, and is added with a proper amount of auxiliary materials such as preservatives and the like, and has the functions of cleaning and sterilizing hands, skin mucous membranes or object surfaces. The disinfection wet tissue is a product which takes non-woven fabric, dust-free paper or other raw materials as a carrier, takes purified water as production water, is added with a proper amount of auxiliary materials such as preservative and the like, and has the cleaning and disinfection effects on hands, skin mucosa, the surface of object medical equipment or the surface of production equipment.

With the increase of the types of air pollution and infectious diseases, the requirement of people on the cleanness and the sanitation of daily life is increasingly increased, and especially, wet tissues are considered to be used before and after meals or under the condition that the people are inconvenient to clean by flowing water. However, there are reports that wet tissues cause allergy because the wet tissues contain various chemical components and the quality of products of some manufacturers is not stable. In addition, the disinfection wet tissues in the market at present contain bactericidal components, kill beneficial bacteria while killing pathogenic bacteria on the surface of the skin, and can stimulate the skin to cause pachylosis, chapping and the like after long-term use.

Disclosure of Invention

The technical problem that this application will be solved provides a wet piece of cloth that security is good, is difficult for taking place unfavorable condition such as allergy under the condition of long-term the use, under the prerequisite that promotes skin surface microbial flora and balance, effectively protects the skin.

The invention aims to solve another technical problem of improving the use convenience and safety of the wet tissue and promoting people to use the wet tissue to prevent infectious diseases.

The application provides a wet tissue which comprises a textile layer, wherein a carrier layer coated with probiotics is arranged on the textile layer; the carrier layer coated with the probiotics contains a carrier coated with the probiotics, and is fixed with the textile fabric layer in a sticking, inlaying, adsorbing, hot-pressing or sewing mode.

Preferably, the textile layer comprises a breathable water-absorbing layer, and the carrier layer coated with the probiotics is arranged on one side for wiping the skin.

For the adhesion of microorganisms to the surface of a material, the structure and properties of the material directly influence the adhesion effect of the microorganisms on the carrier material. Wherein, the adsorption characteristic of the carrier material is closely related to the morphology structure, the pore distribution, the surface chemical property of the material and the like. The solid surface of the carrier material provides an adsorption site for adhesion of microorganisms. The material gaps not only provide growth spaces for microorganisms, are beneficial to the growth and the propagation of the microorganisms, but also are beneficial to the internal and external exchange of substances such as nutrient substances, metabolites, gases and the like. The ideal support material should have a large specific surface area and a high porosity.

Preferably, the carrier coated with the probiotics is one or more of zeolite, kaolin, clay, bentonite and activated carbon.

Preferably, the textile layer is made of petrochemical fibers or plant fibers, such as chemical fibers, polyester fibers, non-woven fabrics, gauze, sponge, viscose, cotton cloth or paper sheets. In some cases, quick-dry fabrics may also be used.

Different sizes can be designed for wet wipes that meet different objectives. For example, a width of 3 to 50cm and a length of 3 to 100cm may be used. As another example, the width is 5, 10, 20, 30cm, the length is 5, 15, 20, 30, 40, 50, 70, 90cm, and so forth.

The carrier layer coated with the probiotics is distributed in the shape of animal outlines, cartoon images, numbers or letters, characters and the like in order to improve the hygiene maintenance enthusiasm of the children when the children use the wet tissues usually for cleaning.

The probiotic bacteria are one or more of lactobacillus acidophilus, lactobacillus bulgaricus, lactobacillus paracasei, lactobacillus fermentum, lactobacillus rhamnosus, bifidobacterium bifidum and bifidobacterium longum.

In the examples of the present application, the probiotic bacteria include Lactobacillus fermentum (i.e., Lactobacillus fermentum, ATCC11976) purchased from ATCC, and Streptococcus thermophilus (i.e., Streptococcus thermophilus, ATCC19258) purchased from shanghai auspicious biotechnology limited.

Preferably, the mixing ratio of lactobacillus fermentum and streptococcus thermophilus is 10: (3-10). For example, the concentration of each of the two bacteria is 3 x 10⁸cfu/ml and 2.5 x 10⁸cfu/ml, 100ml and 80ml may be mixed, respectively.

For example, in a preferred embodiment of the present application, the probiotic bacteria comprise lactobacillus fermentum and streptococcus thermophilus; the quantitative ratio of lactobacillus fermentum to streptococcus thermophilus is 2: 1.

in another aspect, the present application provides a method for preparing the wet tissue, comprising the steps of:

preparing a woven fabric layer according to a predetermined shape;

preparing a carrier layer coated with probiotics, wherein the carrier layer coated with probiotics contains the carrier coated with probiotics; the carrier coated with the probiotics is one or more of zeolite, kaolin, clay, bentonite and activated carbon;

the carrier layer coated with the probiotics is fixed with the textile layer in a sticking, inlaying, adsorbing, hot-pressing or sewing mode, and the carrier layer coated with the probiotics is arranged on one side of the textile layer for wiping.

The probiotic bacteria of the present application may be prepared using conventional technical methods.

Preferably, the preparation method of the carrier coated with probiotics comprises the following steps:

(1) activating and culturing probiotics, wherein the probiotics are lactobacillus fermentum and/or streptococcus thermophilus;

(2) expanding culture solution of probiotics with density of not less than 2 x 10⁸cfu/ml；

(3) Mixing the bacterial liquids to obtain a composite bacterial liquid, and controlling the ratio of lactobacillus fermentum to streptococcus thermophilus to be 10: (3-10);

(4) sterilizing the carrier, and placing the carrier in the compound bacterial liquid;

(5) and (5) freeze-dehydrating the composite bacteria liquid to obtain the carrier coated with the probiotics.

The carrier coated with the probiotics is arranged on one side of the wet tissue for wiping.

Typically, the number of viable probiotic bacteria in a wet wipe is not less than 2 x 10⁷cfu/g。

In order to be convenient to carry and reduce the space occupied by the wet tissue, the wet tissue can be compressed and folded to the volume of not more than 6cm³E.g. 4cm³，3cm³Even 1cm³The following. The wet tissue is compressed into a column shape or a cuboid, and can also be in any shape or shape which is popular with people, such as a spherical shape, a small star shape, an animal shape, a doll shape, a knife shape, a vehicle shape and the like.

For further convenience of use, the wet wipes of the present application are further accompanied by a liquid-containing capsule. The liquid contained therein may be water, a disinfectant or other liquid suitable for use in the preparation of wet wipes.

Preferably, the wet tissue is compressed to form a three-dimensional structure with at least one plane, the water containing capsule also comprises a plane, one plane of the wet tissue is attached to the plane of the capsule, and the two planes are separated by a layer of film. When the wet tissue is used, the compressed wet tissue is pressed, breaks through the film, expands when meeting water, and is opened to be suitable. Meanwhile, the probiotics are activated when meeting water, so that the flora balance of the skin can be effectively improved, and the immune protection effect is achieved.

Correspondingly, the preparation method also comprises the following steps:

compressing and folding wet tissue to volume of not more than 6cm³The compressed wet tissue is basically free of moisture and has a plane;

preparing a liquid-filled capsule, wherein the liquid-filled capsule also comprises a plane, and the plane separates water from the outside by adopting a film;

one plane of the wet tissue is jointed with one plane of the capsule.

Preferably, the above preparation method further comprises the step of drying the wet wipe.

In one embodiment of the invention, the wipes may be compressed into any form that is easily identifiable or preferred, and the liquid-containing capsule is wrapped around the compressed wipes, the portion in contact with the wipes comprising at least one piercing portion. Typically, the compressed wet wipes have a volume less than the internal volume enclosed by the capsule.

The pressure resistance of the packaging material outside the capsule is higher than that of the puncture part, so that the puncture part can be damaged by pressing the capsule with force, liquid can enter the compressed wet tissue, but the outside of the capsule is not broken.

Preferably, the area of one piercing part is equivalent to one side of the capsule, and the wet tissue expands after absorbing water and extends into the capsule.

Preferably, the capsule has a relatively rigid outer shell around its periphery. When the wet tissue is not used, the external force is not enough to press and damage the puncture part under the normal condition because of the shell.

The wet tissue can be used for daily skin cleaning and nursing of crowds, can effectively reduce the phenomena of allergy, red swelling and the like, and is particularly suitable for crowds with weak immunity, such as teenagers and children. The wipes of the present application may also be formulated into products for specific uses, such as cosmetic pads, makeup removers, masks, wound protection dressings, and the like. The wet tissue is particularly suitable for daily skin cleaning of people with low immunity, daily protection of patients with skin diseases such as allergic dermatitis and the like, and tertiary protection of epidemic diseases such as influenza virus, coronavirus and the like.

Drawings

Fig. 1 is a schematic view of a probiotic wet wipe.

Wherein 11 is the wet tissue body, 12 is the carrier layer of the load probiotics.

Fig. 2 is a schematic diagram of a probiotic capsule wet wipe.

Wherein, 21 is the outer package of the probiotic capsule wet tissue, 22 is the compressed wet tissue, 23 is a capsule filled with water, and 24 is a carrier layer loaded with probiotics.

Fig. 3 is a schematic diagram II of a probiotic capsule wet wipe.

Wherein, A is a front view, B is a left view, C is a top view, and D is a perspective view.

In the perspective view, 31 is a water capsule, 32 is a compressed wet tissue, 33 is the internal space of the capsule, and 34 is the hard shell outer package of the probiotic capsule wet tissue.

Detailed Description

The present application will be described in more detail below. This application may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the application to those skilled in the art.

Example 1 probiotic Wet wipes

As shown in figure 1, the application provides a wet tissue, wherein the wet tissue body comprises a non-woven fabric layer, and a carrier layer coated with probiotics is distributed on one side of the non-woven fabric layer for wiping skin. The carrier layer of coating probiotics contains bentonite and the cotton layer of mixing probiotics, and the bentonite of mixing probiotics scatters in one side of whole wet piece of cloth, and the cotton layer is fixed through the hot pressing mode with the non-woven fabrics layer, and the bentonite of mixing probiotics is settled between non-woven fabrics and cotton.

When in use, the packaging bag of the wet tissue is opened, and the wet tissue can be used. The wet tissue is added with probiotic carrier mainly comprising bentonite, can better preserve the activity of probiotic, and is suitable for cleaning and caring skin. Compared with the skin-care skin-cleaning wet tissue prepared by using the disinfectant and chemical components, the skin-care skin-cleaning wet tissue is safer.

Example 2 probiotic capsule Wet wipes I

As shown in fig. 2, the present application provides a compressible wet wipe. The wet tissue is integrally formed when opened, and the textile layer of the wet tissue is provided with a carrier loaded with probiotics. The whole wet tissue is compressed into a cylinder shape after being dried, and the appearance of the water-filled capsule is in a cylinder shape and also comprises a plane. The wet tissue is compressed and placed on the upper part of the water containing capsule, the bottom plane of the wet tissue is attached to the plane of the capsule, the wet tissue and the capsule are separated by a layer of film, and a puncture part is arranged on the film. The probiotics used in the wet tissue are composite probiotics of lactobacillus fermentum and streptococcus thermophilus, wherein the ratio of the lactobacillus fermentum to the streptococcus thermophilus is 2: 1.

when the wet tissue block is used, the package is opened, the compressed wet tissue is pressed, the puncture part on the film is broken, and the wet tissue block expands when meeting water and can be used after being opened. Meanwhile, the probiotics are activated when meeting water, so that flora balance of the skin can be effectively improved. When the wet tissue is not used, the outer package is hard, so that the thin film between the wet tissue and the water containing capsule can be protected from being easily broken.

Example 3 probiotic capsule Wet wipes II

The capsule for loading liquid is approximately cubic, contains commercially available coix seed and towel gourd moisturizing water, and has a cylindrical space surrounded by the capsule. The walls of the cylindrical space are formed by a film and comprise a piercing part. The wet wipe together with the probiotic-loaded carrier thereon is dried and compressed into a heart shape, placed in the interior space of the capsule, with a certain porosity in relation to the walls of the interior space (cylindrical space) of the capsule. The packaging material outside the capsule has hard pressure resistance and is not easy to be extruded and deformed.

When in use, the outer package of the capsule is removed, and the capsule is pressed forcibly to destroy the puncture part. Liquid enters the compressed wet tissue, the wet tissue swells when meeting water, probiotics on the wet tissue are activated when meeting water, but the outside of the capsule is not broken.

Example 4 probiotic Wet wipes reduce the probability of facial skin allergies

To test the improvement effect of the probiotic wet wipes on facial skin, they were made into masks and 100 adults, who often experienced skin allergy, were recruited to test the masks made with the wet wipes of example 1. Before use, the mask is soaked in commercially available coix seed and towel gourd moisturizing water. Wherein 20 adults are used as a control group, the other 80 adults are divided into 4 experimental groups, a carrier layer loaded with probiotics is added on the basis of the common non-woven fabric, and the carrier layer is soaked in the common moisturizing water of the brand. The individuals in the experimental group and the control group draw and assign again every month. The mask was applied twice a week for 15 minutes each time in a month unit.

In the first month, 20 control group members used masks made of ordinary wet tissues, and freeze-dried powders of lactobacillus fermentum were scattered on the masks within 10 minutes before use. The remaining testers (experimental group) used masks made with the wet wipes of example 1, with the lactobacillus fermentum number on the carrier layer being comparable to the control group. In one month, 9 of 20 control group members had red and swollen allergy, and the test was stopped when allergy occurred; and 33 of 80 panelists developed red, swollen and allergic conditions. Therefore, the carrier layer loaded with the probiotics is added to the wet tissue, so that the flora balance and the immunity of the facial skin can be improved.

In the second month, the experimental groups were divided into 4 groups of 20 persons each, and the mask prepared using probiotic wet wipes, wherein the ratio of the number of lactobacillus fermentum to streptococcus thermophilus used was 1: 0.5,1: 1,1: 2,1: 3. in one month, 8 of 20 control group members showed allergic symptoms, and 5, 7, 6 and 8 of 4 experimental group members showed allergic symptoms such as exanthema or red swelling, respectively. Therefore, the combination of the lactobacillus fermentum and the streptococcus thermophilus can better play a role in enhancing the skin immunity, and on the basis, the increase of the proportion of the lactobacillus fermentum is beneficial to promoting the health condition of the face generally.

In the third month, the experimental group was still divided into 4 groups of 20 persons each, and the mask was made using probiotic wet wipes, wherein the ratio of the number of lactobacillus fermentum to streptococcus thermophilus used was 1: 0.3,1: 0.5,1: 0.8,1: 1. in one month, 7 of 20 control group members showed allergic symptoms, and 5, 4, 5, and 7 of 4 experimental group members showed red swelling or rash.

In general, the use of a wet wipe with a carrier layer of probiotic bacteria is effective in promoting skin health.

As those skilled in the art will appreciate, the present application may be embodied in many other specific forms without departing from the spirit or scope thereof. Although embodiments of the present application have been described, it is to be understood that the present application is not limited to those precise embodiments, and that various changes and modifications may be effected therein by one skilled in the art within the spirit and scope of the present application as defined by the appended claims.

Claims

1. A wet tissue comprises a textile layer, and is characterized in that a carrier layer coated with probiotics is arranged on the textile layer;

the carrier layer coated with the probiotics contains a carrier coated with the probiotics;

the carrier coated with the probiotics is one or more of zeolite, kaolin, clay, bentonite and activated carbon, and is fixed with the textile fabric layer in a sticking, inlaying, adsorbing, hot-pressing or sewing mode;

the wet tissue is compressed to a volume of not more than 6cm³The dry three-dimensional structure of (1).

2. The wet wipe as set forth in claim 1 wherein the textile layer is petrochemical or vegetable fiber.

3. The wet wipe as set forth in claim 1 wherein the probiotics comprise lactobacillus fermentum and streptococcus thermophilus; the quantitative ratio of lactobacillus fermentum to streptococcus thermophilus is 10: (3-10).

4. The wet wipe as set forth in claim 1 wherein the probiotic-coated carrier layer is disposed in the central portion of the wet wipe and is disposed in the shape of the outline of an animal.

5. A wet wipe as set forth in claim 1 wherein the probiotic-coated carrier layer is interspersed on both sides of the textile layer.

6. The wet wipe as set forth in claim 1 wherein the probiotic-coated carrier layer further comprises a breathable woven fabric layer;

the breathable textile layer is arranged on one side, used for wiping skin, of the carrier layer coated with the probiotics, and the carrier coated with the probiotics is located between the textile layer of the wet tissue and the breathable textile layer.

7. A wet wipe as claimed in any one of claims 1 to 6 wherein the wet wipe comprises a dry wet wipe as claimed in any one of claims 1 to 6 and water containing capsules.

8. The wet wipe as set forth in claim 7 wherein the wet wipe is compressed to form a dry product and is separated from the water containing capsule by a membrane, the membrane comprising one or more piercing elements.

9. Use of a wet wipe according to any of claims 1-6, characterized in that the wet wipe is used for wiping the skin or a surface in contact with the skin.

10. Use according to claim 9, characterized in that the wet wipes are used for the preparation of cosmetic cottons, make-up removers or masks.