CN111995694A - Method for decoloring and purifying polygonatum kingianum polysaccharide - Google Patents
Method for decoloring and purifying polygonatum kingianum polysaccharide Download PDFInfo
- Publication number
- CN111995694A CN111995694A CN202010832822.1A CN202010832822A CN111995694A CN 111995694 A CN111995694 A CN 111995694A CN 202010832822 A CN202010832822 A CN 202010832822A CN 111995694 A CN111995694 A CN 111995694A
- Authority
- CN
- China
- Prior art keywords
- polysaccharide
- decoloring
- polygonatum kingianum
- water
- purifying
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The invention discloses a method for decoloring and purifying polygonatum kingianum polysaccharide, which comprises the following steps: (1) water extraction; decocting the steamed polygonatum kingianum with water to obtain a water extract; (2) decoloring; loading the water extract on macroporous adsorbent resin of LS-109D, eluting with water, and collecting eluate; (3) concentrating and drying; and concentrating and drying the eluent to obtain the compound. The invention has the advantages that: the obtained Polygonatum kingianum polysaccharide is white, and the polysaccharide content is not less than 60%.
Description
Technical Field
The invention belongs to the technical field of biological medicines, and particularly relates to a method for decoloring and purifying polygonatum kingianum polysaccharide.
Background
Polygonatum kingianum Coll. et Hemsl is a perennial herb of Polygonatum of Liliaceae, is used as a medicine by using roots and stems, is a common traditional Chinese medicine in China, and has the effects of tonifying qi and yin, strengthening spleen and tonifying kidney and the like; modern pharmacological research shows that polygonatum kingianum polysaccharide is one of the main active ingredients, has multiple pharmacological effects of immunoregulation, fatigue resistance, blood sugar reduction, aging resistance and the like, and is widely applied to the industries of medicines, health care products, cosmetics and the like.
Polysaccharides are saccharides with complex and large molecular structures, which are formed by condensation and dehydration of a plurality of monosaccharide molecules. The existing method for extracting polygonatum polysaccharide mainly comprises a water extraction method, alcohol separation, microwave-assisted extraction, ultrasonic extraction and the like, and has the problems of complex extraction components, dark color and the like. The removal of plant pigments mostly adopts activated carbon decolorization, cellulose anion exchange resin decolorization and the like, and has the problems of poor decolorization effect, damage to polysaccharide components, large polysaccharide loss, difficult filtration after decolorization and the like; in addition, the polysaccharide has the problems of strong hygroscopicity, difficult storage, easy decomposition and the like.
Therefore, the development of a preparation method which is rich in high polysaccharide, has good color and appearance and is easy to store is an urgent problem to be solved at present.
Disclosure of Invention
Aiming at the problems, the invention aims to provide a method for decoloring and purifying polygonatum kingianum polysaccharide, which can effectively enrich the polysaccharide.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a method for decoloring and purifying Polygonatum kingianum polysaccharide comprises the following steps:
(1) water extraction; decocting the steamed polygonatum kingianum with water to obtain a water extract;
(2) decoloring; loading the water extract on macroporous adsorbent resin of LS-109D, eluting with water, and collecting eluate;
(3) concentrating and drying; and concentrating and drying the eluent to obtain the compound.
Preferably, in the step (1), the number of times of water decoction is 1-5, and the time of each time of water decoction is 0.5-3 hours.
Preferably, in the step (2), the flow rate of water elution is 1-3 BV/h.
Preferably, in the step (2), the pretreatment of the macroporous adsorption resin with the model number of LS-109D comprises the following steps: and (3) taking LS-109D macroporous adsorption resin, soaking the resin in 95% ethanol for 36-72 hours, and then washing the resin with purified water until no alcohol smell exists for later use.
Preferably, in the step (3), the concentration is performed by open boiling concentration.
Preferably, in the step (3), the drying is performed by freeze drying.
Preferably, in order to reduce the hygroscopicity of the product and facilitate the storage, the maltodextrin is added into the concentrated solution to be freeze-dried, and then the concentrated solution is freeze-dried after being uniformly mixed.
Preferably, the addition amount of the maltodextrin accounts for 20-40% of the weight of the concentrated solution.
The invention also provides polygonatum kingianum polysaccharide prepared by the method.
Preferably, the polygonatum kingianum polysaccharide is white, and the polysaccharide content is higher than 60%.
The invention has the following beneficial effects:
the Polygonatum kingianum polysaccharide obtained after water extraction and decoloration is white, the polysaccharide content is not lower than 60%, and the remarkable technical effect is achieved.
Drawings
FIG. 1 is a process flow diagram of the process for decolorizing and purifying Polygonatum kingianum polysaccharide;
FIG. 2 is a photograph showing the decolorization effect of different types of macroporous adsorbent resins on the water extract of Polygonatum kingianum;
FIG. 3 is a photograph of the product obtained in examples 1 to 3;
FIG. 4 is a graph showing the result of detection of polysaccharide in the product of the present invention.
Detailed Description
The following further describes embodiments of the present invention with reference to the drawings. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
Example 1
As shown in figure 1, a method for decoloring and purifying polygonatum kingianum polysaccharide comprises the following steps:
(1) water extraction; decocting the steamed Polygonatum kingianum with water twice, the first time for 1.5 hr, the second time for 1 hr, mixing the decoctions, filtering with 300 mesh sieve, collecting filtrate, and concentrating to obtain water extract (crude extract);
(2) decoloring; and (3) taking LS-109D macroporous adsorption resin, soaking the resin in 95% ethanol for 48 hours, filling the resin into a column by a wet method, and washing the resin by purified water until no alcohol smell exists. Loading the water extract onto LS-109D macroporous adsorbent resin, and collecting the effluent; adding purified water for elution, wherein the flow rate of water washing is 2BV/h, and collecting eluent;
(3) concentrating and drying; mixing the effluent and eluate, boiling, and concentrating to obtain concentrated solution (decolorized extract). Dissolving the decolorized extract with appropriate amount of water, adding maltodextrin, mixing, freeze drying, pulverizing, and sieving with 80 mesh sieve (see figure 3). Wherein the addition amount of maltodextrin accounts for 30% of the weight of the decolorized extract.
Example 2
As shown in figure 1, a method for decoloring and purifying polygonatum kingianum polysaccharide comprises the following steps:
(1) water extraction; decocting the steamed Polygonatum kingianum with water for 5 times, each for 1 hr, mixing the decoctions for 5 times, filtering with 300 mesh sieve, collecting filtrate, and concentrating to obtain water extract (crude extract);
(2) decoloring; and (3) taking LS-109D macroporous adsorption resin, soaking for 72 hours by using ethanol with the volume percentage concentration of 95%, filling columns by using a wet method, and then washing by using purified water until no alcohol smell exists. Loading the water extract onto LS-109D macroporous adsorbent resin, and collecting the effluent; adding purified water for elution, wherein the flow rate of water washing is 3BV/h, and collecting eluent;
(3) concentrating and drying; mixing the effluent and eluate, boiling, and concentrating to obtain concentrated solution (decolorized extract). Dissolving the decolorized extract with appropriate amount of water, adding maltodextrin, mixing, freeze drying, pulverizing, and sieving with 80 mesh sieve (see figure 3). Wherein the addition amount of maltodextrin accounts for 20% of the weight of the decolorized extract.
Example 3
As shown in figure 1, a method for decoloring and purifying polygonatum kingianum polysaccharide comprises the following steps:
(1) water extraction; decocting the steamed Polygonatum kingianum with water for 3 hr, filtering the decoction with 300 mesh screen, collecting filtrate, and concentrating to obtain water extract (crude extract);
(2) decoloring; and (3) taking LS-109D macroporous adsorption resin, soaking for 36 hours by using ethanol with the volume percentage concentration of 95%, filling columns by using a wet method, and then washing by using purified water until no alcohol smell exists. Loading the water extract onto LS-109D macroporous adsorbent resin, and collecting the effluent; adding purified water for elution, wherein the flow rate of water washing is 1BV/h, and collecting eluent;
(3) concentrating and drying; mixing the effluent and eluate, boiling, and concentrating to obtain concentrated solution (decolorized extract). Dissolving the decolorized extract with appropriate amount of water, adding maltodextrin, mixing, freeze drying, pulverizing, and sieving with 80 mesh sieve (see figure 3). Wherein the addition amount of maltodextrin accounts for 40% of the weight of the decolorized extract.
Comparative examples 1 to 4
The macroporous adsorbent resin model LS-109D of example 1 was replaced with AB-8, D101, LS-305 and LS-160B, respectively. The same procedure was followed to give comparative example 1, comparative example 2, comparative example 3 and comparative example 4, respectively. The decolorization effect is shown in FIG. 2.
As can be seen from FIG. 2, the color appearance of the obtained decolorized filtrate is, from left to right, the filtrate which is obtained after the decolorization treatment is carried out on the decolorized filtrate and 5 macroporous resins of AB-8, D101, LS-305, LS-109D and LS-160B. As shown in FIG. 2, LS-109D and LS-160 showed better decolorization effect.
In order to verify the technical effect of the invention, the polysaccharide yield and the polysaccharide loss rate of products treated by different models are measured, and the results are shown in table 1.
TABLE 15 comparison table of polysaccharide yield and polysaccharide loss after decolorization of macroporous adsorbent resin of different models
| Resin type | Polysaccharide yield (%) | Polysaccharide loss ratio (%) |
| AB-8 | 67.99 | 32.01 |
| D101 | 80.06 | 19.94 |
| LS-305 | 78.59 | 21.41 |
| LS-109D | 95.66 | 4.34 |
| LS-160B | 55.04 | 44.96 |
As can be seen from Table 1, the macroporous adsorbent resin with the model LS-109D has the highest polysaccharide yield and the lowest polysaccharide loss rate.
Wherein, the polysaccharide yield (%) is decolorized extract polysaccharide content/un-decolorized extract polysaccharide content x 100%
Polysaccharide loss rate (%) -. 100% -polysaccharide yield%
In the invention, the polysaccharide content is determined by a sulfuric acid-anthrone method, and the specific method comprises the following steps:
preparation of control solutions: accurately weighing 100mg of standard glucose dried to constant weight at 105 ℃, placing in a 100ml volumetric flask, adding purified water to scale, shaking up, and preparing into standard glucose mother liquor (stored at 4 ℃) with the concentration of 1 mg/ml. Precisely measuring 10.0ml of mother liquor, placing the mother liquor in a 50ml volumetric flask, adding purified water to the scale, and shaking up to obtain the standard glucose use solution with the concentration of 0.1 mg/ml.
Preparing a color developing solution: 0.2g of anthrone reagent is precisely weighed, transferred into a beaker, and 100ml of concentrated sulfuric acid is slowly added, carefully stirred by a glass rod, and stored in the dark for 1 hour before use. The preparation is ready for use in each test.
Preparation of a test solution:
1) and (3) testing the sample: accurately weighing 10.0mg of the product obtained in the example 1 into a 50ml colorimetric tube, adding water to dissolve the product and fixing the volume to the scale, shaking up, accurately transferring 1.0ml to a 10ml colorimetric tube, adding water to fix the volume to the scale, and shaking up to obtain the product.
2) Dextrin sample: accurately weighing 5.0mg of maltodextrin (added to a sample, type Glucosidex 19D) in a 50ml colorimetric tube, adding water to dissolve and fix the volume to scale, shaking up, accurately transferring 2.0ml to 10ml colorimetric tube, adding water to fix the volume to scale, and shaking up to obtain the final product.
Detection of
The test tubes were numbered according to Table 2, the corresponding solutions were added, and the procedure was followed. No. 0-5 is a standard curve, and S1 and S2 are respectively a detection sample and a maltodextrin to be detected.
TABLE 2
The absorbance values of the other tubes were measured at a wavelength of 620nm using tube 0 as a blank reference.
Drawing a standard curve: a standard curve was drawn with the glucose concentration (. mu.g/ml) as the abscissa and the absorbance value as the ordinate (the glucose concentration here is the final concentration of the prepared control solution after adding purified water and a color-developing solution as listed in "Table 2").
Computing: the total polysaccharide content (%) of the sample was (C1 × 2500 × 10)-3/M1-C2×1250×10-3/M2×50％)×100％
C1-content of sample solution (μ g/ml) measured and calculated by standard curve
C2-content of dextrin sample solution (. mu.g/ml), calculated from a standard curve
M1-quality of test sample (mg)
M2-dextrin sample Mass (mg)
The detection result is shown in FIG. 4; the raw data are shown in table 3.
TABLE 3
According to the original data, C1 is 6.2640ug/ml, C2 is 6.9270ug/ml, and the calculation formula is substituted into the Yunnan Siberian solomonseal rhizome total polysaccharide content calculation formula, so that the Yunnan Siberian solomonseal rhizome total polysaccharide content is 65.38%.
To summarize: the polygonatum kingianum extract freeze-dried powder which is rich in polysaccharide, light in color and easy to store can be obtained by 3 steps of water extraction, LS-109D resin decolorization and maltodextrin freeze-drying, the preparation process is simple and easy to operate, the extraction cost is low, the problems of complex production process, polysaccharide loss, difficult decolorization, easy moisture absorption and the like are well solved, and the polygonatum kingianum extract freeze-dried powder is more suitable for industrial production.
The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, and the scope of protection is still within the scope of the invention.
Claims (10)
1. A method for decoloring and purifying Polygonatum kingianum polysaccharide is characterized in that: the method comprises the following steps:
(1) water extraction; decocting the steamed polygonatum kingianum with water to obtain a water extract;
(2) decoloring; loading the water extract on macroporous adsorbent resin of LS-109D, eluting with water, and collecting eluate;
(3) concentrating and drying; and concentrating and drying the eluent to obtain the compound.
2. The method for decoloring and purifying polygonatum kingianum polysaccharide according to claim 1, wherein the method comprises the following steps: in the step (1), the water decoction times are 1-5 times, and the water decoction time is 0.5-3 hours each time.
3. The method for decoloring and purifying polygonatum kingianum polysaccharide according to claim 1, wherein the method comprises the following steps: in the step (2), the flow rate of water elution is 1-3 BV/h.
4. The method for decoloring and purifying polygonatum kingianum polysaccharide according to claim 1, wherein the method comprises the following steps: in the step (2), the pretreatment of the macroporous adsorption resin with the model of LS-109D comprises the following steps: and (3) taking LS-109D macroporous adsorption resin, soaking the resin in 95% ethanol for 36-72 hours, and then washing the resin with purified water until no alcohol smell exists for later use.
5. The method for decoloring and purifying polygonatum kingianum polysaccharide according to claim 1, wherein the method comprises the following steps: in the step (3), the concentration is carried out in an open boiling concentration mode.
6. The method for decoloring and purifying polygonatum kingianum polysaccharide according to claim 1, wherein the method comprises the following steps: in the step (3), the drying is performed by freeze drying.
7. The method for decoloring and purifying polygonatum kingianum polysaccharide according to claim 6, wherein the method comprises the following steps: and adding maltodextrin into the concentrated solution to be freeze-dried, uniformly mixing, and freeze-drying.
8. The method for decoloring and purifying polygonatum kingianum polysaccharide according to claim 7, wherein the method comprises the following steps: the addition amount of the maltodextrin accounts for 20-40% of the weight of the concentrated solution.
9. A polygonatum kingianum polysaccharide prepared by the method of any one of claims 1 to 8.
10. The polygonatum kingianum polysaccharide of claim 9, wherein: the Polygonatum kingianum polysaccharide is white, and the polysaccharide content is higher than 60%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010832822.1A CN111995694B (en) | 2020-08-18 | 2020-08-18 | Method for decoloring and purifying polygonatum kingianum polysaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010832822.1A CN111995694B (en) | 2020-08-18 | 2020-08-18 | Method for decoloring and purifying polygonatum kingianum polysaccharide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111995694A true CN111995694A (en) | 2020-11-27 |
| CN111995694B CN111995694B (en) | 2022-07-01 |
Family
ID=73472604
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010832822.1A Active CN111995694B (en) | 2020-08-18 | 2020-08-18 | Method for decoloring and purifying polygonatum kingianum polysaccharide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111995694B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114306190A (en) * | 2022-01-25 | 2022-04-12 | 云南贝泰妮生物科技集团股份有限公司 | Polygonatum kingianum extract as well as preparation method and application thereof |
| CN115043955A (en) * | 2022-06-29 | 2022-09-13 | 西南林业大学 | Polygonatum polysaccharide extract and preparation method thereof |
| CN115261427A (en) * | 2022-09-27 | 2022-11-01 | 云南英格生物技术有限公司 | Sealwort fermented oligosaccharide and preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104910443A (en) * | 2015-05-11 | 2015-09-16 | 南昌大学 | Preparation method of spray-dried dendrobium polysaccharide powder |
| CN107739314A (en) * | 2017-10-24 | 2018-02-27 | 西安蓝深环保科技有限公司 | A kind of decoloration of glycin mother liquid method |
| CN107827995A (en) * | 2017-12-18 | 2018-03-23 | 大理大学 | A kind of method for extraction and purification of P. kingianum polysaccharide |
| CN107854864A (en) * | 2017-10-24 | 2018-03-30 | 陕西蓝深特种树脂有限公司 | A kind of phenylglycine produces mother liquor decolorizing process technique |
| CN108077683A (en) * | 2017-12-03 | 2018-05-29 | 西北大学 | A kind of production method for the depickling jujube juice that decolourizes |
-
2020
- 2020-08-18 CN CN202010832822.1A patent/CN111995694B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104910443A (en) * | 2015-05-11 | 2015-09-16 | 南昌大学 | Preparation method of spray-dried dendrobium polysaccharide powder |
| CN107739314A (en) * | 2017-10-24 | 2018-02-27 | 西安蓝深环保科技有限公司 | A kind of decoloration of glycin mother liquid method |
| CN107854864A (en) * | 2017-10-24 | 2018-03-30 | 陕西蓝深特种树脂有限公司 | A kind of phenylglycine produces mother liquor decolorizing process technique |
| CN108077683A (en) * | 2017-12-03 | 2018-05-29 | 西北大学 | A kind of production method for the depickling jujube juice that decolourizes |
| CN107827995A (en) * | 2017-12-18 | 2018-03-23 | 大理大学 | A kind of method for extraction and purification of P. kingianum polysaccharide |
Non-Patent Citations (5)
| Title |
|---|
| 侯双菊: "黄精多糖的提取工艺及提取液树脂法脱色的研究", 《中国优秀博硕士学位论文全文数据库(硕士) 工程科技Ｉ辑》 * |
| 安雅琦等: "大孔树脂吸附处理苯胺废水及再生方法试验研究", 《煤炭加工与综合利用》 * |
| 张春燕等: "利用大孔树脂对莼菜多糖脱色的工艺优化", 《食品工业科技》 * |
| 张洪坤: "大孔树脂吸附纯化茯苓多糖工艺研究", 《食品研究与开发》 * |
| 潘永康等: "《现代干燥技术》", 31 May 2007, 化学工业出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114306190A (en) * | 2022-01-25 | 2022-04-12 | 云南贝泰妮生物科技集团股份有限公司 | Polygonatum kingianum extract as well as preparation method and application thereof |
| CN114306190B (en) * | 2022-01-25 | 2023-09-12 | 云南贝泰妮生物科技集团股份有限公司 | Polygonatum kingianum extract and preparation method and application thereof |
| CN115043955A (en) * | 2022-06-29 | 2022-09-13 | 西南林业大学 | Polygonatum polysaccharide extract and preparation method thereof |
| CN115261427A (en) * | 2022-09-27 | 2022-11-01 | 云南英格生物技术有限公司 | Sealwort fermented oligosaccharide and preparation method and application thereof |
| CN115261427B (en) * | 2022-09-27 | 2022-12-16 | 云南英格生物技术有限公司 | Sealwort fermented oligosaccharide and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111995694B (en) | 2022-07-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111995694B (en) | Method for decoloring and purifying polygonatum kingianum polysaccharide | |
| CN110693930B (en) | Method for extracting high-purity panax notoginseng saponins | |
| CN101229207B (en) | Decoloring refined technology of notoginseng total saponin duolite method | |
| CN102258588B (en) | Preparation method of peony general glycoside | |
| CN101336949B (en) | Method for extracting polysaccharide and flavone from Gynura divaricata | |
| CN111187366B (en) | Double-aqueous-phase extraction method of polygonatum sibiricum polysaccharide | |
| CN1282654C (en) | Process for preparing astraglus base total saponin | |
| CN102423329B (en) | A kind of discoloration method of panax notoginsenoside extract | |
| CN102631414B (en) | SepHaniadelavayi Diels total alkaloid extraction and purification technology | |
| CN108888669B (en) | Preparation method of oral liquid for clearing heat and relieving cough of children | |
| CN104530253A (en) | Polygonatum sibiricum polysaccharide column chromatography separation method and extraction method | |
| CN107213180B (en) | Separation and extraction method of notoginseng flavone | |
| CN102002087A (en) | Method for preparing bryonia alcohol acid | |
| CN111548380A (en) | Preparation method of monotropein in morinda officinalis | |
| CN102920785A (en) | Method for extracting total flavone from guava leaves | |
| CN1272331C (en) | Method of extracting effective components in kudzu vine root | |
| CN113440547B (en) | Method for separating and purifying Japanese thistle herb total glycosides by adopting macroporous resin series dynamic axial compression column | |
| CN114617913A (en) | Method for extracting, separating and purifying total saponins of astragalus stems and leaves | |
| CN112121071B (en) | A product prepared from Ginseng radix | |
| CN104771713A (en) | Mulberry-ginger extract, and preparation process and application thereof | |
| CN108186773B (en) | Weight-losing and lipid-lowering medicinal preparation and preparation method thereof | |
| CN112043733A (en) | Production method of water-soluble ginkgo leaf extract | |
| CN106336440A (en) | Method for extracting and separating out oleanolic acid from olive leaves | |
| CN110025645A (en) | A method of extracting American ginseng total saponins | |
| NL2026582B1 (en) | Treatment method for improving saponin from aerial part of panax notoginseng |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |