CN111995066A - Method for treating petrochemical industry wastewater by adopting biological enhanced microbial inoculum adsorption-embedding mode - Google Patents
Method for treating petrochemical industry wastewater by adopting biological enhanced microbial inoculum adsorption-embedding mode Download PDFInfo
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- CN111995066A CN111995066A CN202010863067.3A CN202010863067A CN111995066A CN 111995066 A CN111995066 A CN 111995066A CN 202010863067 A CN202010863067 A CN 202010863067A CN 111995066 A CN111995066 A CN 111995066A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/34—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32
- C02F2103/36—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32 from the manufacture of organic compounds
- C02F2103/365—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32 from the manufacture of organic compounds from petrochemical industry (e.g. refineries)
Abstract
The invention discloses a method for treating petrochemical industry wastewater by adopting a biological strengthening microbial inoculum adsorption-embedding mode, which comprises the steps of firstly immobilizing functional microbial inoculum by adopting an adsorption material, and then embedding a material adsorbed with active microorganisms. The novel active biological granular material can be directly added to quickly start a petrochemical industry wastewater biochemical treatment system, so that good wastewater treatment efficiency is obtained, frequent adding is not needed, and the persistence is more than several months.
Description
Technical Field
The invention relates to the field of microbial wastewater treatment, in particular to a method for treating petrochemical wastewater by adopting a biological enhanced microbial inoculum adsorption-embedding mode.
Background
The petrochemical industry is vital to economic development and national life of China, but a large amount of refractory organic wastewater is generated in the processes of mining, processing and chemical conversion of petroleum, coal, natural gas and other petrochemical energy sources, and the wastewater usually contains a large amount of refractory organic matters such as cyanogen, quinoline, pyridine, indole, long-chain hydrocarbons, aromatic compounds and the like and has high salt content. The wastewater must be purified before being discharged in order to prevent pollution.
At present, an activated sludge mode is usually adopted for wastewater treatment, a common activated sludge method is long in acclimatization time and poor in efficiency, functional bacteria obtained by adding and screening can be biologically enhanced to efficiently start a wastewater treatment system, but the problems of easy loss of functional microorganisms, poor environmental adaptability and the like exist, and the duration after adding is short. The embedding and immobilizing technology can effectively immobilize functional microorganisms, but due to the problem of effective mass transfer of organic matters and oxygen in embedded particles, the service life and the activity of the microorganisms need to be balanced, and the high level is difficult to achieve simultaneously. Therefore, the conventional embedding and immobilizing mode is not very suitable for treating the wastewater in the petrochemical industry, and the conventional wastewater treatment method in the petrochemical industry needs to be further improved.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention aims to provide a method for embedding a biological strengthening microbial inoculum, which has higher organic matter removal efficiency and longer service life.
A method for treating petrochemical industry wastewater by adopting a biological enhanced microbial inoculum adsorption-embedding mode comprises the following steps:
1) screening or enriching functional bacteria or flora according to the water quality characteristics of the target petrochemical wastewater;
2) carrying out propagation culture on the functional bacteria or flora obtained in the step 1) to prepare a biological strengthening microbial inoculum;
3) mixing the biological strengthening microbial inoculum obtained by the propagation in the step 2) with an adsorption material, and carrying out adsorption immobilization;
4) embedding and immobilizing the adsorption material adsorbed with the active microorganisms in the step 3);
5) and (3) adding the novel active biological particle material embedded and solidified in the step 4) into a petrochemical industry wastewater biochemical treatment system for wastewater treatment.
Preferably, the petrochemical industry wastewater is wastewater generated in chemical conversion processes of petroleum, coal and natural gas petrochemical energy exploitation, processing, refining and refining.
Preferably, the functional bacteria or flora in the step 1) are selected from single bacteria, mixed bacteria and activated sludge which are obtained by screening and enriching waste water in the petrochemical industry.
Preferably, the biological enhancement microbial inoculum in the step 2) is one or more of bacteria, fungi and other functional strains capable of efficiently degrading the non-degradable substances such as target organic compounds cyanogen, quinoline, pyridine, indole, long-chain hydrocarbons, aromatic compounds and the like.
Preferably, in the step 2), the bioaugmentation microbial inoculum expansion process can be respectively and purely culturing different microorganisms or expanding mixed microorganisms together.
Preferably, in step 3), the adsorbing material is selected from one or a mixture of more of powdered activated carbon, biochar, diatomite, fly ash, gypsum, zeolite, clay, ceramsite, magnetic particles, volcanic rock, bamboo charcoal porous material or high molecular organic material.
Preferably, in step 4), the embedding material for embedding and immobilizing is one or a mixture of more of high molecular compound materials such as sodium alginate, chitosan, polyvinyl alcohol, polyacrylamide or polyurethane.
Preferably, in step 5), the petrochemical industry wastewater biochemical treatment system is a primary or advanced treatment system such as an activated sludge tank, a biological contact oxidation tank, a membrane bioreactor, a biological filter or a biological rotating disk.
Preferably, in the step 5), the embedded and immobilized novel active biological particle material can be directly added when being added to a wastewater treatment system, or can be added after being subjected to activation culture.
The technical scheme has the following beneficial effects: compared with the common embedding method, the adsorption-embedding co-immobilized active biological particle material has higher organic matter removal efficiency and longer service life. The novel active biological granular material can be directly added to quickly start a petrochemical industry wastewater biochemical treatment system, so that good wastewater treatment efficiency is obtained, frequent adding is not needed, and the persistence is more than several months.
Drawings
FIG. 1 is a graph showing the effect comparison between the conventional embedding method and the wastewater treatment method of this patent.
Detailed Description
The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will become apparent to those skilled in the art from the present disclosure.
The example embodiments may be embodied in many different forms and should not be construed as limited to the examples set forth herein; rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the concept of example embodiments to those skilled in the art. The described features, structures, or characteristics may be combined in any suitable manner in one or more embodiments. In the following description, numerous specific details are provided to provide a thorough understanding of embodiments of the invention. One skilled in the relevant art will recognize, however, that the invention may be practiced without one or more of the specific details, or with other methods, components, devices, steps, and so forth. In other instances, well-known technical solutions have not been shown or described in detail to avoid obscuring aspects of the invention.
The petrochemical industry wastewater treated by the wastewater treatment method is mainly wastewater generated in chemical conversion processes of petroleum, coal and natural gas petrochemical energy exploitation, processing, refining and the like. The waste water contains the non-degradable substances such as organic cyanogen, quinoline, pyridine, indole, long-chain hydrocarbon, aromatic compounds and the like. The wastewater treatment method of this patent will be described in detail with reference to specific examples.
Example one
Shale gas fracturing flowback liquid (COD 520 mg/L, ammonia nitrogen 210 mg/L and salinity 3.2%) is used as a wastewater treatment object, and the specific treatment method comprises the following steps:
1) screening functional bacteria according to water quality characteristics, adding yeast powder into wastewater for enrichment for 3 times, coating on a limiting agar plate prepared from shale gas fracturing flowback fluid, and selecting single bacterial colonies to test one by one to obtain the functional bacteria with high degradation efficiency.
2) Then the screened functional bacteria are expanded and cultured, 5 high-efficiency functional bacteria are respectively placed in 30 YPD culture mediumsoC, expanding culture in a shaking table at 150 rpm for 24 hours.
3) And (3) mixing the microbial bacteria liquid obtained by propagation culture, adding an adsorption material, and uniformly mixing 250 mL of the microbial bacteria liquid with 10 g of powdered activated carbon for adsorption immobilization.
4) Then adding 700 mL of sodium alginate solution (30 g/L), 10 mL of oleic acid and 805 mL of Tween and an adsorbing material adsorbing active microorganisms, uniformly stirring, and carrying out embedding immobilization to prepare an active microorganism functional material as a novel active biological particle material, wherein the active microorganism functional material is adsorbed and embedded and co-immobilized.
5) And finally, directly adding the active microorganism functional material subjected to adsorption-embedding co-fixation into a biochemical treatment system for the shale gas fracturing flow-back fluid wastewater to perform wastewater treatment. The biochemical treatment system for the shale gas fracturing flow-back wastewater is a primary or advanced treatment system such as an activated sludge tank, a biological contact oxidation tank, a membrane bioreactor, a biological filter or a biological rotating disk, and the biochemical treatment system for the wastewater in the petrochemical industry is the prior art, so the detailed description is not needed.
Example two
Coal chemical wastewater (COD 720 mg/L, ammonia nitrogen 10 mg/L, salinity 5.8%) is used as a wastewater treatment object, and the specific treatment method comprises the following steps:
1) firstly enriching functional microbial flora, adding yeast powder into the wastewater for enrichment for 6 times, and then placing the enriched high-efficiency functional microbial flora in a YPD culture medium at 30oC, expanding culture in a shaking table at 150 rpm for 24 hours; then 250 mL of bacterial liquid and 5 g of powder are takenUniformly mixing the activated carbon, and carrying out adsorption immobilization; adding 700 mL of 10% polyvinyl alcohol solution, uniformly mixing, and dripping into saturated boric acid solution with pH of 6.8 to obtain solution 4oStanding for 24 h, and carrying out embedding immobilization to prepare an active microorganism functional material which is adsorbed, embedded and co-immobilized; the prepared adsorption-embedding co-immobilized active microorganism functional material is activated by using 20 g/L molasses and then is added into a biochemical treatment system for treating the high-salinity wastewater in the coal chemical industry.
The method for treating the wastewater in the petrochemical industry firstly adopts an adsorption material to immobilize functional microorganism liquid and then embeds the material adsorbed with active microorganisms. Referring to fig. 1, compared with the general embedding method, the adsorption-embedding co-immobilized active biological particle material has higher organic matter removal efficiency and longer service life; and the novel active biological granular material can be directly added to quickly start a petrochemical industry wastewater biochemical treatment system, so that good wastewater treatment efficiency is obtained, frequent adding is not needed, and the persistence is more than several months.
The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.
Claims (9)
1. A method for treating petrochemical industry wastewater by adopting a biological enhanced microbial inoculum adsorption-embedding mode is characterized by comprising the following steps: which comprises the following steps:
1) screening or enriching functional bacteria or flora according to the water quality characteristics of the target petrochemical wastewater;
2) carrying out propagation culture on the functional bacteria or flora obtained in the step 1) to prepare a biological strengthening microbial inoculum;
3) mixing the biological strengthening microbial inoculum obtained by the propagation in the step 2) with an adsorption material, and carrying out adsorption immobilization;
4) embedding and immobilizing the adsorption material adsorbed with the active microorganisms in the step 3);
5) and (3) adding the novel active biological particle material embedded and immobilized in the step 4) into a petrochemical industry wastewater biochemical treatment system for wastewater treatment.
2. The method for treating petrochemical industry wastewater by adopting a bioaugmented microbial inoculum adsorption-embedding mode according to claim 1, which is characterized in that: the petrochemical industry wastewater is wastewater generated by refining and refining in the processes of petroleum, coal and natural gas petrochemical energy exploitation, processing and chemical conversion.
3. The method for treating petrochemical industry wastewater by adopting a bioaugmented microbial inoculum adsorption-embedding mode according to claim 1, which is characterized in that: the functional bacteria or flora in the step 1) are single bacteria, mixed bacteria and activated sludge which are screened or enriched by using the waste water in the petrochemical industry.
4. The method for treating petrochemical industry wastewater by adopting a bioaugmented microbial inoculum adsorption-embedding mode according to claim 1, which is characterized in that: the biological strengthening microbial inoculum in the step 2) is one or more of bacteria, fungi and other functional strains which can efficiently degrade refractory substances such as target organic compounds, such as cyanogen, quinoline, pyridine, indole, long-chain hydrocarbons, aromatic compounds and the like.
5. The method for treating petrochemical industry wastewater by adopting a bioaugmented microbial inoculum adsorption-embedding mode according to claim 1, which is characterized in that: in the step 2), different microorganisms can be respectively and purely cultured or mixed microorganisms can be simultaneously cultured in the process of the amplification culture of the biological enhanced microbial inoculum.
6. The method for treating petrochemical industry wastewater by adopting a bioaugmented microbial inoculum adsorption-embedding mode according to claim 1, which is characterized in that: in the step 3), the adsorbing material is selected from one or a mixture of more of powdered activated carbon, biochar, diatomite, fly ash, gypsum, zeolite, clay, ceramsite, magnetic particles, volcanic rock, bamboo charcoal porous material or organic polymer material.
7. The method for treating petrochemical industry wastewater by adopting a bioaugmented microbial inoculum adsorption-embedding mode according to claim 1, which is characterized in that: in the step 4), the embedding material for embedding and immobilizing is one or a mixture of more of high molecular compound materials such as sodium alginate, chitosan, polyvinyl alcohol, polyacrylamide or polyurethane.
8. The method for treating petrochemical industry wastewater by adopting a bioaugmented microbial inoculum adsorption-embedding mode according to claim 1, which is characterized in that: in the step 5), the embedded and immobilized novel active biological particle material can be directly added when being added into a wastewater treatment system, or can be added after being subjected to activation culture.
9. The method for treating petrochemical industry wastewater by adopting a bioaugmented microbial inoculum adsorption-embedding mode according to claim 1, which is characterized in that: in the step 5), the petrochemical industry wastewater biochemical treatment system is a primary or advanced treatment system such as an activated sludge tank, a biological contact oxidation tank, a membrane bioreactor, a biological filter or a biological rotating disk.
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Cited By (4)
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| CN112619615A (en) * | 2020-12-16 | 2021-04-09 | 中国矿业大学(北京) | Preparation method of biochar-microorganism composite material and method for treating tailing wastewater |
| CN112745858A (en) * | 2020-12-15 | 2021-05-04 | 广东华南环保产业技术研究院有限公司 | Persistent organic pollutant contaminated site repairing agent and preparation method thereof |
| CN114214310A (en) * | 2021-12-14 | 2022-03-22 | 天津大学 | Modified biochar immobilized petroleum degrading bacteria microsphere as well as preparation method and application thereof |
| CN115010245A (en) * | 2022-05-11 | 2022-09-06 | 安徽理工大学 | Chemical wastewater treating agent, preparation method and application thereof |
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| CN114214310A (en) * | 2021-12-14 | 2022-03-22 | 天津大学 | Modified biochar immobilized petroleum degrading bacteria microsphere as well as preparation method and application thereof |
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| CN115010245B (en) * | 2022-05-11 | 2023-09-12 | 安徽理工大学 | Chemical wastewater treatment agent, preparation method and application thereof |
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Inventor after: Han Xiongshen Inventor after: Li Li Inventor after: Jin Yan Inventor after: Shi Fengyang Inventor after: Fan Congfeng Inventor after: Cao Zhijun Inventor before: Han Xiushen Inventor before: Li Li Inventor before: Jin Yan Inventor before: Shi Fengyang Inventor before: Fan Congfeng Inventor before: Cao Zhijun |
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Application publication date: 20201127 |