Background
Cardiovascular disease is the disease most harmful to the human life health in modern society, and about 1790 million people die of cardiovascular disease in 2016, accounting for 31% of the total death in the world, according to the report of the world health organization. Thus, the medical need for new drugs for the prevention and treatment of cardiovascular diseases is very urgent. Related researches prove that the TRPC channel is an important pharmacological target for developing new drugs for cardiovascular diseases such as cardiomyopathy, heart failure, hypertension, cerebrovascular diseases and the like. The patent application WO2006/074802A1 discloses that TRPC channel is used for treating cardiovascular and cerebrovascular diseases, and research shows that by using gene technology, in a rabbit atherosclerosis model, the vascular function and vascular pathological changes of atherosclerosis can be obviously improved by inhibiting the activities of vascular endothelial cells TRPC3\ TRPC6 and TRPC 7.
The TRPC channel is a Ca2+The permeated non-selective ion channel is widely present in mammalian tissues. The TRPC family can be divided into four subgroups based on structural homology and functional predilection: TRPC1 and TRPC2 each constituted a subset; TRPC4 and TRPC5 have approximately 65% amino acid homology between them, thus dividing them into the same subpopulation; TRPC3, TRPC6 and TRPC7 have 70-80% amino acid homology and are classified into the same subgroup. TRPC3, TRPC6 and TRPC7 channels share a common activation mechanism, and the endogenous ligands thereof are known to be Diacylglycerol (DAG) and 4-ethyl- (3- (4-fluorophenyl) -7-hydroxy-2-methylpyrazole [1,5-a ]]-pyrimidin-5-yl) piperidine-1-carboxylic acid salt (M085). Organic inhibitors of TRPC known to date are 2-aminoethoxydiphenylboronic acid (2-APB), SKF96365, YM-58483(BTP2) and inorganic blockers (e.g. Gd)3+And La3 +) Etc., but all lack sufficient effectiveness and specificity. The natural composition, activation mechanism, physiological function and their use of TRPC are now involvedThe role in pathophysiology and disease, etc., remains a pending problem. Achieving in situ identification of native TRPC channels presents certain difficulties because of their widespread and partially overlapping distribution, potential heteropolymerization, similar electrophysiological properties, and the lack of tools for compounds that specifically track these channels.
Studies by Dietrich et al demonstrated that it is possible to unravel some of the TRPC's possible physiological functions by studying transgenic mouse models, summarizing the hetero-ultimate potential of TRPC3, 6, 7 subfamilies in vitro and in vivo, and provide preliminary data for their analysis of physiological functions in isolated tissue and gene-deficient mouse models with down-regulated channel activity. However, due to the lack of specific channel blockers, which are susceptible to compensatory effects from channels closely related to the TRPC channel, it is impossible to determine the physiological relevance of TRPC isoforms or isoforms in the function of complex organs throughout the body, and to overcome this drawback, targeted gene inactivation in embryonic stem cells and subsequent production of gene-deficient mouse models for each channel and channel subfamily are required, and the generation and analysis of these model systems is very time-consuming and costly, and has certain limitations.
Abelmoschus Manihot is dry flower of Abelmoschus Manihot (L.) Medic of Abelmoschus of Malvaceae, is recorded in Jiayouben, is widely distributed and has abundant resources, and is recorded in Ben compendium of materia Medica: the flowers have sweet, cold, smooth and nontoxic smell, are mainly used for treating urinary stranguria and hastening the growth of the patients, are used for treating patients with malignant sores and pus for a long time and are healed after being applied for powder, are the main medicines for sores, eliminate gangrene swelling, soak oil and spread soup for fire injury and the like.
Abelmoschus manihot contains various chemical components including: gallic acid, 5-hydroxymethyl-2-furancarboxylic acid, protocatechuic acid-3-O-beta-D-glucoside, protocatechuic acid, acortarin A, gossypetin-3-O-beta-D-glucose-8-O-beta-D-glucuronide, quercetin-3-O- [ beta-D-xylosyl (1 → 2) -alpha-L-rhamnosyl 1 → 6) ] -beta-D-galactoside, myricetin-3-O-beta-D-glucoside, quercetin-3-O-beta-D-xylosyl- (1 → 2) -beta-D-galactoside, quercetin-3-O-locust glucoside, rutin, hyperoside, isoquercitrin, myricetin-3 '-O-beta-D-glucoside, gossypetin-8-O-beta-D-glucuronide, myricetin, quercetin-3' -O-beta-D-glucoside, quercetin and the like.
At present, no research shows that the inhibition effect of quercetin-3-O-robinin on TRPC channel and the application thereof in preparing the medicine for treating cardiovascular diseases, coronary heart disease, atherosclerosis, end-stage renal failure, neurological diseases, chronic pain, acute pain or inflammatory diseases related to the TRPC channel are provided.
In view of the above, the invention provides the use of the abelmoschus manihot extract as an inhibitor of a TRPC ion channel and the preparation of a medicament for treating cardiovascular diseases, coronary heart diseases, atherosclerosis, end-stage renal failure, neurological diseases, chronic pain, acute pain or inflammatory diseases, and proves that quercetin-3-O-robioside in the extract is used as a new pharmacological tool, can selectively inhibit the TRPC ion channel, can be distinguished between TRPC subfamilies and inside TRPC subfamilies, so that the effects of different channels under physiological and pathophysiological conditions can be clarified, ideas for cardiovascular and cerebrovascular diseases and the like are developed, and the use of the abelmoschus manihot extract is expanded.
Disclosure of Invention
The technical scheme of the invention is as follows:
the terms:
1. the terms "TRPC channel", "TRPC ion channel" or "TRPC" in the context of the present invention refer to Ca permeable2+Non-selective cation channels of (a). It refers to any one of the following list of transient receptor potentials typical ion channels: TRPC1, TRPC2, TRPC3, TRPC4, TRPC5, TRPC6 and TRPC 7. Particularly preferred are TRPC3, TRPC6 and TRPC 7.
Such TRPC ion channels may be derived from any vertebrate, and in particular mammalian species (e.g., dog, horse, cow, mouse, rat, dog, rabbit, chicken, ape, human, or others). TRPC can be isolated from tissue probes of such vertebrate organisms or produced by methods of recombinant biomaterials capable of expressing TRPC proteins.
The term may refer to native polypeptides, polymorphic variants, mutants, and interspecies homologs.
2. The term "pharmacological tool" in the context of the present invention refers to compounds and combinations of compounds whose functional properties enable the study of how drugs interact with living organisms to produce changes in the function of interest, thereby enabling the study of new pharmaceutical compositions, as well as the properties, interactions, toxicology, therapy, medically and antiviral abilities. Moreover, the term refers to compounds that can be used to characterize potential targets in new drug development, e.g., to characterize their natural components, activation mechanisms, physiological functions, and roles in pathophysiology and disease.
3. The term "TRPC ion channel modulator" in the context of the present invention refers to a regulatory molecule of the TRPC channel, in particular an inhibitory or activating molecule ("inhibitor" or "activator"), in particular an inhibitor of the TRPC channel identifiable according to the method of the present invention. The inhibitor is typically a compound, as described in detail preferably above, for example, that binds to, partially or totally blocks activity, reduces, prevents, delays activation, inactivates, desensitizes or down regulates the activity or expression of at least one TRPC channel. An activator is generally a compound, as described in detail preferably above, for example, that increases, opens, activates, promotes, enhances activation, sensitizes, agonizes or upregulates the activity or expression of at least one TRPC channel. Such modulators include genetically engineered versions of the TRPC channel, preferably inactivating mutants of the TRPC channel, as well as naturally occurring or synthetic ligands, antagonists, agonists, peptides, cyclic peptides, nucleic acids, antibodies, antisense molecules, ribozymes, small organic molecules, and the like. Examples of TRPC activators are diacylglycerol, in particular 1-oleoyl-2 acetyl-sn-glycerol (OAG); gq-coupled receptor agonists, such as phenylephrine, particularly trypsin; agonists that stimulate receptor tyrosine kinases such as Epidermal Growth Factor (EGF); or a diacylglycerol-producing enzyme such as a phospholipase or an activator thereof. Examples of measurements of modulation of TRPC ion channel activity in the presence of test compounds are as follows: generally, a cell expressing a TRPC channel is provided. Such cells can be produced using genetic methods known to those skilled in the art. After performing inducible expression of the TRPC channel, it will generally beThe cells are placed in, for example, a microplate and grown. Typically cells are grown and fixed at the bottom of a multi-well plate. The cells are then washed routinely and a dye, preferably a fluorescent dye such as fluo4am, is added in a suitable loading buffer. After removal of the loading buffer, the cells are incubated with test compounds or modulators (in particular the biochemical or chemical test compounds mentioned above, for example in the form of chemical compound libraries Ca2+The measurement can be performed by using, for example, a fluorescence imaging plate reader (FLIPR). To stimulate Ca2+ influx through the TRPC channel, channel activators such as OAG and 4-ethyl- (3- (4-fluorophenyl) -7-hydroxy-2-methylpyrazole [1,5-a ] are generally used]-pyrimidin-5-yl) piperidine-1-carboxylic acid salt (M085). The expected effect of the inhibitor is, for example, a decrease in the increase in fluorescence. An activator may result in, for example, a further increase in fluorescence induced by the activator, or induce, for example, an increase in fluorescence independent of the activator. Thereafter, suitable modulators, in particular inhibitors, can be analyzed and/or isolated. Screening of chemical compound libraries is preferably performed using high throughput assays known to the skilled artisan or commercially available.
4. The term "TRPC expressing cell" in the context of the present invention refers to a cell or recombinant cell endogenously expressing an ion channel of interest. The cell is typically a mammalian cell, such as a human cell, a mouse cell, a rat cell, a chinese hamster cell, and the like. Cells which have been found to be convenient include MDCK, HEK293, HEK 293T, BHK, COS, NIH3T3, Swiss3T3 and CHO cells, preferably HEK293 cells.
5. The term "tissue" in the context of the present invention refers to any type of tissue preparation, or a part of a tissue or organ (e.g. brain, liver, spleen, kidney, heart, blood vessels, muscle, skin etc., also refers to any type of bodily fluid such as blood, saliva, lymph, synovial fluid etc.), preferably if derived from a vertebrate, more preferably from a mammal such as a human. Tissue samples can be obtained by well-known techniques, such as blood sampling, tissue lancing, or surgical techniques.
6. The term "drug" in the context of the present invention refers to a therapeutic agent comprising a therapeutically effective amount of quercetin-3-O-robioside, or a plant extract comprising these compounds. The drug can be administered systemically or locally in any conventional manner. This can be done, for example, by means of oral dosage forms such as tablets, granules or capsules, by means of mucous membranes such as the nasal or buccal cavity, depot preparations for subcutaneous implantation, by means of injections, infusions or gels containing the medicaments according to the invention. If appropriate, for the treatment of a particular disease as mentioned above, it is also possible to administer the drug locally (topocally and locally) in the form of a liposome complex. The drug may also be administered in the form of an injection or infusion solution, and if only a relatively small amount of solution or suspension, for example about 1 to 20mL, is administered to the body, usually using an injection solution.
In one aspect, the invention provides the use of quercetin-3-O-robinin in the preparation of a medicament for the treatment of a calcium channel mediated disease.
As an example, the invention provides the application of quercetin-3-O-robinin in preparing a medicament for inhibiting a calcium ion channel.
As an illustrative or preferred example, the calcium channel is a TRPC channel (or referred to as a TRPC ion channel).
As an illustrative or preferred example, the TRPC channel TRPC3, TRPC6 or TRPC7 channel described above.
As an illustrative or preferred example, the above calcium channel refers to a calcium channel used by quercetin-3-O-robioside for inhibition in vitro and in vivo.
In another aspect, the present invention provides the use of quercetin-3-O-robinin in the preparation of a medicament for the diagnosis, treatment or adjunctive treatment of cardiovascular disease, coronary heart disease, atherosclerosis, end-stage renal failure, neurological disease, chronic pain, acute pain or inflammatory disease.
By way of example, the present invention provides the use of quercetin-3-O-robinin in the manufacture of a medicament for the diagnosis, treatment or adjunct treatment of cardiovascular disease, coronary heart disease, atherosclerosis, end stage renal failure, neurological disease, chronic pain, acute pain or inflammatory disease.
As an illustrative or preferred example, the medicament may be formulated with one or more pharmaceutically acceptable carriers or adjuvants. Pharmaceutically acceptable carriers or adjuvants are, for example, physiological buffer solutions such as sodium chloride solutions, demineralised water, stabilisers such as protease or nuclease inhibitors, or chelating agents such as EDTA.
In another aspect, the present invention provides a plant extract comprising more than 0.2% by weight of quercetin-3-O-robioside; further, the plant extract contains 0.2-1.2% by weight of quercetin-3-O-robioside; further, the plant extract contains 0.4-0.8% by weight of quercetin-3-O-robioside; preferably, the plant is selected from Hibiscus plant and Malvaceae plant, and more preferably one or more of Abelmoschus manihot, Hibiscus viniferus, Hibiscus esculentus and Phyllanthus niruri.
As an illustrative or preferred example, the plant extract is an extract of abelmoschus manihot. The flos Abelmoschi Manihot flower extract is ethanol extract, preferably 50-95% ethanol reflux extract, and more preferably 80-95% ethanol reflux extract.
The abelmoschus manihot flower extract can be prepared by the following method: heating and refluxing flos Abelmoschi Manihot with ethanol, filtering, concentrating the filtrate, and drying. Further, it is preferably prepared by the following method: extracting the flower of abelmoschus manihot with 85-95% ethanol under reflux for 1-3 times, each time for 1-2 hours, filtering, combining the filtrates, recovering ethanol, concentrating the filtrate until the specific gravity is 1.20-1.35, standing the concentrated solution at 0-4 ℃ for 24-48 hours, removing an oil layer of a refrigerating solution, adjusting the pH value to 6.0-7.0, concentrating, and performing thin-layer quick drying or vacuum drying to obtain the flower of abelmoschus manihot extract.
The preparation method of the abelmoschus manihot flower extract can comprise the following steps: extracting flos Abelmoschi Manihot with 95% ethanol under reflux for 2 times, each for 1 hr, filtering, mixing filtrates, recovering ethanol, concentrating the filtrate to specific gravity of 1.20, standing the concentrated solution at 0-4 deg.C for 24-48 hr, removing oil layer of the cold storage solution, adjusting pH to 6.0, slowly adding the cold storage solution into thin-layer quick drying roller tank until the liquid level of the cold storage solution in the roller tank just contacts with the surface of the roller, preheating the surface temperature of the roller to 140-150 deg.C, and controlling air pressure to 0.4-0.5 Mpa, turning on the roller rolling start button, controlling the rotation speed of the roller to 3-3.5 min/r, coating the rolled extract liquid on a polytetrafluoroethylene plate to cool, cooling to brittleness, breaking, and packaging into clean double-layer plastic bags to obtain flos Abelmoschi Manihot extract.
The conditions of the thin layer rapid drying operation are as follows: the surface temperature of the preheating thin layer quick drying roller body is 135-160 ℃, the air pressure is 0.3-0.6 Mpa, the rotating speed of the roller is 2-4.5 minutes/revolution, the coating plate is a plastic plate or a stainless steel plate, the plastic plate is selected from a polyethylene plate, a PVC plastic plate, a PP plastic plate, a PE plastic plate and a polytetrafluoroethylene plate, and the polytetrafluoroethylene plate is preferred.
The preferable preparation method of the abelmoschus manihot flower extract comprises the following steps: extracting flos Abelmoschi Manihot with 95% ethanol under reflux for 2 times, each for 1 hr, filtering, mixing filtrates, recovering ethanol, concentrating the filtrate to specific gravity of 1.20, standing the concentrated solution at 0-4 deg.C for 24-48 hr, removing oil layer of the cold storage solution, adjusting pH to 6.0, concentrating, and slowly adding into vacuum belt drier for vacuum belt drying.
As an example, the preparation method of the abelmoschus manihot extract comprises the following steps: taking 4000g of abelmoschus manihot as a medicinal material, performing reflux extraction for 2 times by using 15 times (mass/volume ratio) of 95% ethanol, performing 1 hour each time, filtering, combining filtrates, recovering the ethanol, concentrating the filtrate to the specific gravity of 1.20, standing the concentrated solution for 24 hours at the temperature of 0-4 ℃, removing an oil layer of a refrigerating solution, adjusting the pH value to 6.0, slowly adding the concentrated solution into a drier after concentration, drying, crushing, and filling into a clean double-layer plastic bag to obtain the abelmoschus manihot extract.
In another aspect, the present invention provides a sunflower flower extract comprising more than 0.2% by weight quercetin-3-O-robioside; further, the flower extract of abelmoschus manihot contains 0.2-1.2% by weight of quercetin-3-O-robioside; further, the sunflower flower extract contains 0.4-0.8% by weight quercetin-3-O-robioside.
On the other hand, the invention provides the application of the abelmoschus manihot extract in preparing a medicament for inhibiting a calcium ion channel.
In another aspect, the present invention provides the use of the above abelmoschus manihot extract in the manufacture of a medicament for the diagnosis, treatment or adjuvant treatment of cardiovascular diseases, coronary heart disease, atherosclerosis, end-stage renal failure, neurological diseases, chronic pain, acute pain or inflammatory diseases.
In another aspect, the present invention provides a medicament for the treatment or co-treatment of cardiovascular disease, coronary heart disease, atherosclerosis, end stage renal failure, neurological disease, chronic pain, acute pain, or inflammatory disease, said medicament comprising quercetin-3-O-robioside.
As an example, the present invention provides a medicament for the treatment or co-treatment of cardiovascular disease, coronary heart disease, atherosclerosis, end-stage renal failure, neurological disease, chronic pain, acute pain, or inflammatory disease, said medicament comprising quercetin-3-O-robioside.
In another aspect, the present invention provides a new pharmacological tool that is able to distinguish between and within TRPC subfamilies. Thereby enabling elucidation of the role of the different channels under physiological and pathophysiological conditions. That is, the present invention provides a pharmacological tool for characterizing channels belonging to different TRPC subfamilies, said pharmacological tool comprising quercetin-3-O-robioside.
According to the present invention, this is achieved by inhibiting TRPC3, TRPC6 and TRPC7 with quercetin-3-O-robioside. Thus, quercetin-3-O-robioside enables pharmacological differentiation of channels belonging to different TRPC subfamilies. In addition, quercetin-3-O-robioside does not interfere with the common G protein-coupled receptor, Gq, phospholipase C β pathway that mediates TRPC channel activation in many cells. These properties make quercetin-3-O-robioside a preferred tool for identifying and modulating TRPC3, TRPC6 and TRPC 7.
As an inhibitor of TRPC3, TRPC6 and TRPC7, quercetin-3-O-robioside was used as a pharmacological tool to characterize channels belonging to different TRPC subfamilies, distinguishing TRPC3/6/7 subfamily members from other ion channel family members (fig. 1-6).
As such an inhibitor, quercetin-3-O-robioside may further be used as a tool compound for developing and validating assays to measure activity of the ion channel of interest. An example of such an assay is shown in FIGS. 1-3.
In another aspect, the present invention provides: use of quercetin-3-O-robioside for differential analysis of channel function of TRPC3/6/7 subfamily members under physiological and pathological conditions. This can be done as described in the examples. The assay may be performed in cells, tissues or animals. The animal may be a rodent, preferably a mouse or a rat.
According to a preferred embodiment, the modulation of native TRPC by quercetin-3-O-robioside can be studied using the HEK293 cell line, wherein the HEK293 cell line is a validated model system for studying endogenously expressed TRPC ion channels. Further details of such a preferred assay system are given in the examples and in FIGS. 1-3.
In another aspect, the present invention provides: a method for determining the effect of quercetin-3-O-robioside on TRPC channel activity, preferably TRPC ion channels TRPC3, TRPC6 and TRPC 7.
Generally, cells expressing a TRPC ion channel are contacted with quercetin-3-O-robioside, and the effect of quercetin-3-O-robioside on TRPC ion channel activity is measured or detected.
In another aspect, the present invention provides: preferred TRPC ion channels for the method of identifying a TRPC ion channel modulator are TRPC3, TRPC6 and TRPC 7.
Generally, a cell expressing a TRPC ion channel is contacted with a test compound, and the effect of the test compound on the TRPC ion channel activity is measured or detected.
In embodiments, the cells used in the above methods are fluorescent cells.
Preferred cells according to the invention are MDCK, HEK293, HEK 293T, BHK, COS, NIH3T3, Swiss3T3 or CHO cells, in particular HEK293 cells.
The activity of TRPC channels can be measured by, for example, the patch clamp technique, whole cell current, radiolabeled ion flux, or in particularMeasuring or detecting ion flux, particularly Ca, by fluorescence (e.g. using a voltage-or ion-sensitive dye)2+The change in ion current is measured or detected.
An example of an assay for TRPC channel activity is an assay comprising the following steps:
(1) contacting quercetin-3-O-robioside with fluorescent cells expressing TRPC ion channels and stimulating Ca with a channel activator before, simultaneously with or after the contacting2+Internal flow;
(2) detecting a change in TRPC ion channel activity.
In another aspect, the present invention provides: directed to methods of describing the selectivity of quercetin-3-O-robioside for the TRPC channel, comprising assessing the ability of quercetin-3-O-robioside to inhibit the TRPC channel activity.
The invention has the following beneficial effects:
(1) the invention provides a new application of quercetin-3-O-robinin, an application in preparing a medicament for inhibiting a calcium ion channel and a related application thereof.
(2) Provides a new idea for preparing medicines for treating cardiovascular diseases, coronary heart diseases, atherosclerosis, end-stage renal failure, neurological diseases, chronic pain, acute pain and inflammatory diseases particularly related to TRPC channels and developing selective inhibitors of TRPC ion channels;
(3) the application of the abelmoschus manihot extract is expanded.