CN111979120B - Rabies vaccine nucleic acid digestion removal device and experiment method - Google Patents
Rabies vaccine nucleic acid digestion removal device and experiment method Download PDFInfo
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- CN111979120B CN111979120B CN201910433004.1A CN201910433004A CN111979120B CN 111979120 B CN111979120 B CN 111979120B CN 201910433004 A CN201910433004 A CN 201910433004A CN 111979120 B CN111979120 B CN 111979120B
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
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- C12N2760/00011—Details
- C12N2760/20011—Rhabdoviridae
- C12N2760/20111—Lyssavirus, e.g. rabies virus
- C12N2760/20151—Methods of production or purification of viral material
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- Y02A40/70—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in livestock or poultry
Abstract
The invention discloses a rabies vaccine nucleic acid digestion and removal device and an experimental method, which comprises a bottom plate, an operation box, a box door, a charging hole, a switch, an operation table, an operation panel, a heat dissipation hole, an adjusting box, a vaccine liquid injection pipe, a PBS buffer liquid injection pipe, a driving motor, a rotating shaft, a microfiltration hollow fiber membrane, a first stirring blade, a second stirring blade, an electromagnetic valve, an ultrafiltration hollow fiber membrane, a pH test head, a salt concentration test head, a pH value tester, a salt concentration tester, a liquid guide pump, a treatment tank, a partition plate, a power supply, a spring, damping cotton, an electric box, an exchange chamber, a deactivation chamber, an enzyme treatment chamber, a purification chamber, an anion exchange medium port, an inactivating agent port, a heating wire, a restrictive endonuclease port, a gel filtration column and a storage chamber, wherein the operation box is arranged at one end of the top of the bottom plate, and the partition plate is symmetrically welded in the middle of the inner wall of the operation box, the nucleic acid digestion removal effect is good, the device structure is simple, and the operation is convenient.
Description
Technical Field
The invention relates to the technical field of rabies vaccine production, in particular to a rabies vaccine nucleic acid digestion removal device and an experimental method.
Background
Rabies is an acute infectious disease caused by rabies virus, is commonly suffered by human and animals, is mostly seen in carnivores such as dogs, wolves, cats and the like, is infected by being bitten by the sick animals, and is clinically manifested as unique water fear, wind fearfulness, pharyngeal muscle spasm, progressive paralysis and the like.
Disclosure of Invention
The invention aims to provide a rabies vaccine nucleic acid digestion removal device and an experimental method, so as to solve the problems in the background technology.
In order to solve the technical problems, the invention provides the following technical scheme: a rabies vaccine nucleic acid digestion and removal device comprises a bottom plate, an operation box, a box door, a charging hole, a switch, an operation table, an operation panel, heat dissipation holes, an adjusting box, a vaccine liquid injection pipe, a PBS buffer liquid injection pipe, a driving motor, a rotating shaft, a microfiltration hollow fiber membrane, a first stirring blade, a second stirring blade, an electromagnetic valve, an ultrafiltration hollow fiber membrane, a pH test head, a salt concentration test head, a pH value tester, a salt concentration tester, a first injection port, a second injection port, a liquid guide pump, a treatment tank, a partition plate, a power supply, a spring, damping cotton, an electric box, an exchange chamber, a deactivation chamber, an enzyme treatment chamber, a purification chamber, an anion exchange medium port, an inactivator port, a heating wire, a limiting endonuclease enzyme port, a gel filtration column and a storage chamber, wherein the operation box is arranged at one end of the top of the bottom plate, the partition plate is symmetrically welded in the middle of the inner wall of the operation box, and the power supply and the electric box are respectively arranged on the partition plate, the inner wall bottom symmetry of control box is provided with the spring, the spring has the shock attenuation cotton with baffle space department packing, control box one side is provided with the regulating box, pH value tester and salt concentration tester are installed respectively to regulating box one side, first filling opening and second filling opening have been seted up respectively to the corresponding both ends in the middle of the regulating box surface, the corresponding both ends in top one side of regulating box have welded vaccine liquid injection tube and PBS buffer solution injection tube respectively, the welding of inner wall top of regulating box has driving motor, driving motor's output respectively with first stirring leaf and second stirring leaf fixed connection through the pivot, first stirring leaf top is provided with the micro-filtration hollow fiber membrane, and PBS buffer solution injection tube bottom one end stretches into micro-filtration hollow fiber membrane below, be provided with the solenoid valve in the middle of the inner wall of regulating box, the super-filtration hollow fiber membrane is installed to the below of solenoid valve, the utility model discloses a pH test device, including regulating box, processing jar, anion exchange chamber, enzyme treatment room, pH test head and salt concentration test head are installed respectively to inner wall bottom one side of regulating box corresponding both ends, the regulating box opposite side is passed through the priming pump and is connected with the processing jar, processing jar inner wall top is provided with the exchange room, anion exchange medium mouth has been seted up to exchange room one side, the exchange room bottom is provided with the room of deactivating, the inactivator mouth has been seted up to room one side of deactivating, the room bottom of deactivating is provided with the enzyme treatment room, restriction endonuclease mouth has been seted up to enzyme treatment room one side, the heater strip has all been installed at the middle one side of enzyme treatment room corresponding both ends, enzyme treatment room bottom is provided with the purification room, be provided with the gel filtration post in the middle of the purification room, purification room bottom is provided with the apotheca.
An experimental method of a rabies vaccine nucleic acid digestion removal device comprises the steps of firstly, filtering and concentrating vaccine liquid; adjusting the pH value and the salt concentration; step three, anion exchange; step four, inactivation; step five, enzyme treatment; step six, purifying;
in the first step, vaccine liquid is introduced into a regulating tank through a vaccine liquid injection pipe, the vaccine liquid is subjected to primary clarification and filtration through a microfiltration hollow fiber membrane to form filtrate, PBS buffer liquid is injected into the filtrate through a PBS buffer liquid injection pipe to form mixed liquid, a switch is turned on, a driving motor works, a first stirring blade and a second stirring blade are driven to rotate through a rotating shaft, the mixed liquid is fully stirred, an electromagnetic valve is turned on, and the mixed liquid is subjected to secondary clarification and filtration through an ultrafiltration hollow fiber membrane to obtain the treated vaccine liquid;
in the second step, the pH value tester and the salt concentration tester work, the pH value and the salt concentration of the treated vaccine solution are tested through the pH test head and the salt concentration test head, hydrochloric acid and sodium hydroxide solution are added into the adjusting box through the first injection port to adjust the pH value of the vaccine solution, and sodium chloride solution is added into the adjusting box through the second injection port to adjust the salt concentration of the vaccine solution;
in the third step, the vaccine liquid with the pH value and the salt concentration adjusted is introduced into an exchange chamber of a treatment tank through a liquid guide pump, and an anion exchange medium is injected into the exchange chamber through an anion exchange medium port to carry out anion exchange treatment;
in the fourth step, the vaccine liquid after anion exchange treatment enters an inactivation chamber, an inactivator is injected into the inactivation chamber through an inactivator port for inactivation for 24-36 hours, and the pH value of an inactivation system is controlled to be stabilized between 7.0 and 7.4 in the inactivation process;
in the fifth step, the inactivated vaccine liquid enters an enzyme treatment chamber, the restriction endonuclease is injected into the enzyme treatment chamber through a restriction endonuclease port, nucleic acid is digested, a heating wire works, the temperature of the vaccine liquid is adjusted to be 30-37 ℃, and the vaccine liquid is kept stand for 24 hours;
and in the sixth step, the vaccine liquid after standing enters a purification chamber, gel filtration treatment is carried out through a gel filtration column, nucleic acid is further removed, and the vaccine liquid after removal enters a storage chamber for storage.
According to the technical scheme, one side of the surface of the operation box is hinged with the box door through the hinge, and the handle is welded on one side of the surface of the box door.
According to the technical scheme, the charging hole is formed in one end of the top of one side of the surface of the operation box, the switch is installed at the other end of the top of one side of the surface of the operation box, the operation table is arranged at the top of the operation box, and the operation panel is arranged on one side of the surface of the operation table.
According to the technical scheme, the bottom of one side of the surface of the operation box is uniformly provided with the heat dissipation holes.
According to the technical scheme, in the first step, the aperture of the microfiltration hollow fiber membrane is 0.45 mu m, and the aperture of the ultrafiltration hollow fiber membrane is 300 KD.
According to the technical scheme, in the second step, the pH value of the vaccine liquid is adjusted to be 6-8, and the salt concentration is 0.2-0.5 mol/mol.
According to the technical scheme, in the fourth step, the inactivating agent is beta-propiolactone, and accounts for 0.05-0.2% of the volume of the vaccine liquid.
Compared with the prior art, the invention has the following beneficial effects: according to the invention, the vaccine liquid is filtered and concentrated through the microfiltration hollow fiber membrane and the ultrafiltration hollow fiber membrane, the anion exchange treatment is carried out on the vaccine liquid after the pH value and the salt concentration of the vaccine liquid are adjusted, the nucleic acid is adsorbed, the inactivated vaccine liquid digests the nucleic acid in the vaccine liquid through the restriction endonuclease, the nucleic acid is changed into small fragments, and the nucleic acid gel filtration treatment is carried out through the cross-linked glucan or agarose balls in the gel filtration column, so that the nucleic acid is further removed, the digestion removal effect is good, the device structure is simple, and the operation is convenient.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of the construction of the operation box of the present invention;
FIG. 3 is a schematic view of the structure of the conditioning tank of the present invention;
FIG. 4 is a schematic view of the construction of a treatment tank of the present invention;
FIG. 5 is a flow chart of a method of the present invention;
in the figure: 1. a base plate; 2. an operation box; 3. a box door; 4. a charging hole; 5. a switch; 6. an operation table; 7. an operation panel; 8. heat dissipation holes; 9. an adjusting box; 10. a vaccine solution injection tube; 11. a PBS buffer solution injection tube; 12. a drive motor; 13. a rotating shaft; 14. micro-filtration of the hollow fiber membrane; 15. a first stirring blade; 16. a second stirring blade; 17. an electromagnetic valve; 18. ultrafiltration hollow fiber membranes; 19. a pH test head; 20. a salt concentration test head; 21. a pH value tester; 22. a salt concentration tester; 23. a first injection port; 24. a second injection port; 25. a liquid guiding pump; 26. a treatment tank; 27. a partition plate; 28. a power source; 29. a spring; 30. damping cotton; 31. an electric box; 32. an exchange chamber; 33. a deactivation chamber; 34. an enzyme treatment chamber; 35. a purification chamber; 36. an anion exchange media port; 37. an inactivator port; 38. heating wires; 39. a restriction endonuclease nick; 40. a gel filtration column; 41. a storage chamber.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
Referring to fig. 1-4, the present invention provides a technical solution: a rabies vaccine nucleic acid digestion and removal device comprises a bottom plate 1, an operation box 2, a box door 3, a charging hole 4, a switch 5, an operation table 6, an operation panel 7, a heat dissipation hole 8, an adjusting box 9, a vaccine liquid injection pipe 10, a PBS buffer liquid injection pipe 11, a driving motor 12, a rotating shaft 13, a microfiltration hollow fiber membrane 14, a first stirring blade 15, a second stirring blade 16, an electromagnetic valve 17, an ultrafiltration hollow fiber membrane 18, a pH test head 19, a salt concentration test head 20, a pH value tester 21, a salt concentration tester 22, a first injection port 23, a second injection port 24, a liquid guide pump 25, a treatment tank 26, a partition plate 27, a power supply 28, a spring 29, shock absorption cotton 30, an electric box 31, an exchange chamber 32, a deactivation chamber 33, an enzyme treatment chamber 34, a purification chamber 35, an anion exchange medium port 36, an inactivator port 37, a heating wire 38, a restrictive endonuclease port 39, a gel filtration column 40 and a storage chamber 41, an operation box 2 is arranged at one end of the top of a bottom plate 1, a partition plate 27 is symmetrically welded in the middle of the inner wall of the operation box 2, a power supply 28 and an electric box 31 are respectively arranged on the partition plate 27, springs 29 are symmetrically arranged at the bottom of the inner wall of the operation box 2, damping cotton 30 is filled in gaps between the springs 29 and the partition plate 27, an adjusting box 9 is arranged at one side of the operation box 2, a pH value tester 21 and a salt concentration tester 22 are respectively arranged at one side of the adjusting box 9, a first injection port 23 and a second injection port 24 are respectively arranged at corresponding two ends at the middle of the surface of the adjusting box 9, a vaccine liquid injection pipe 10 and a PBS buffer liquid injection pipe 11 are respectively welded at corresponding two ends at one side of the top of the adjusting box 9, a driving motor 12 is welded at the top of the inner wall of the adjusting box 9, the output end of the driving motor 12 is respectively fixedly connected with a first stirring blade 15 and a second stirring blade 16 through a rotating shaft 13, a microfiltration hollow fiber membrane 14 is arranged above the first stirring blade 15, one end of the bottom of a PBS buffer solution injection pipe 11 extends into the lower part of a microfiltration hollow fiber membrane 14, an electromagnetic valve 17 is arranged in the middle of the inner wall of an adjusting box 9, an ultrafiltration hollow fiber membrane 18 is arranged below the electromagnetic valve 17, a pH test head 19 and a salt concentration test head 20 are respectively arranged at the corresponding two ends of one side of the bottom of the inner wall of the adjusting box 9, the other side of the adjusting box 9 is connected with a processing tank 26 through a liquid guide pump 25, an exchange chamber 32 is arranged at the top of the inner wall of the processing tank 26, an anion exchange medium port 36 is arranged at one side of the exchange chamber 32, an inactivation chamber 33 is arranged at the bottom of the exchange chamber 32, an inactivation agent port 37 is arranged at one side of the inactivation chamber 33, an enzyme processing chamber 34 is arranged at the bottom of the inactivation chamber 33, a restrictive endonuclease port 39 is arranged at one side of the enzyme processing chamber 34, heating wires 38 are respectively arranged at the corresponding two ends of one side of the middle of the enzyme processing chamber 34, a purification chamber 35 is arranged at the bottom of the enzyme processing chamber 34, a gel filter column 40 is arranged in the middle of the purification chamber 35, the bottom of the purification chamber 35 is provided with a storage chamber 41.
Referring to fig. 5, the present invention provides a technical solution: an experimental method of a rabies vaccine nucleic acid digestion removal device comprises the steps of filtering and concentrating vaccine liquid; adjusting the pH value and the salt concentration; step three, anion exchange; step four, inactivation; step five, enzyme treatment; step six, purification;
in the first step, vaccine liquid is introduced into a regulating box 9 through a vaccine liquid injection pipe 10, the vaccine liquid is subjected to primary clarification and filtration through a microfiltration hollow fiber membrane 14 to form filtrate, a PBS buffer liquid is injected into the filtrate through a PBS buffer liquid injection pipe 11 to form mixed liquid, a switch 5 is turned on, a driving motor 12 works, a rotating shaft 13 drives a first stirring blade 15 and a second stirring blade 16 to rotate, the mixed liquid is fully stirred, an electromagnetic valve 17 is turned on, and the mixed liquid is subjected to secondary clarification and filtration through an ultrafiltration hollow fiber membrane 18 to obtain the treated vaccine liquid;
in the second step, the pH tester 21 and the salt concentration tester 22 work, the pH tester 19 and the salt concentration tester 20 test the pH and the salt concentration of the treated vaccine solution, hydrochloric acid and a sodium hydroxide solution are added into the adjusting box 9 through the first injection port 23 to adjust the pH of the vaccine solution, and a sodium chloride solution is added into the adjusting box 9 through the second injection port 24 to adjust the salt concentration of the vaccine solution;
in the third step, the vaccine liquid with the adjusted pH value and salt concentration is introduced into the exchange chamber 32 of the treatment tank 26 through the liquid guide pump 25, and an anion exchange medium is injected into the exchange chamber 32 through the anion exchange medium port 36 for anion exchange treatment;
in the fourth step, the vaccine liquid after anion exchange treatment enters the inactivation chamber 33, an inactivator is injected into the inactivation chamber 33 through an inactivator port 37 for inactivation for 24-36 hours, and the pH of the inactivation system is controlled to be stabilized between 7.0-7.4 in the inactivation process;
in the fifth step, the inactivated vaccine liquid enters the enzyme treatment chamber 34, the restriction endonuclease is injected into the enzyme treatment chamber 34 through the restriction endonuclease port 39 to digest nucleic acid, the heating wire 38 works, the temperature of the vaccine liquid is adjusted to be 30-37 ℃, and the vaccine liquid is kept stand for 24 hours;
in the sixth step, the vaccine liquid after standing enters the purification chamber 35, and is subjected to gel filtration treatment through the gel filtration column 40, so that nucleic acid is further removed, and the vaccine liquid after removal enters the storage chamber 41 for storage.
According to the technical scheme, one side of the surface of the operation box 2 is hinged with the box door 3 through the hinge, and one side of the surface of the box door 3 is welded with the handle.
According to the technical scheme, the charging hole 4 is opened to the top one end of surface one side of the operation box 2, the switch 5 is installed to the top other end of surface one side of the operation box 2, the top of the operation box 2 is provided with the operation table 6, and the operation panel 7 is arranged on one side of the surface of the operation table 6.
According to the technical scheme, the bottom of one side of the surface of the operation box 2 is uniformly provided with the heat dissipation holes 8.
According to the technical scheme, in the first step, the aperture of the microfiltration hollow fiber membrane 14 is 0.45 μm, and the aperture of the ultrafiltration hollow fiber membrane 18 is 300 KD.
According to the technical scheme, in the second step, the pH value of the vaccine liquid is adjusted to be 6-8, and the salt concentration is adjusted to be 0.2-0.5 mol/mol.
According to the technical scheme, in the fourth step, the inactivating agent is beta-propiolactone, and accounts for 0.05-0.2% of the volume of the vaccine liquid.
Based on the above, the present invention has the advantages that the vaccine liquid is filtered and concentrated through the microfiltration hollow fiber membrane 14 and the ultrafiltration hollow fiber membrane 18, the pH value and the salt concentration of the vaccine liquid are adjusted, then the anion exchange treatment is performed on the vaccine liquid, the nucleic acid is adsorbed, the inactivated vaccine liquid digests the nucleic acid in the vaccine liquid through the restriction endonuclease, the nucleic acid is changed into small fragments, the nucleic acid gel filtration treatment is performed through the cross-linked dextran or agarose beads in the gel filtration column 40, the nucleic acid is further removed, the digestion and removal effects are good, the device structure is simple, and the operation is convenient.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, article, or apparatus.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described above, or equivalents may be substituted for elements thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (5)
1. The utility model provides a rabies bacterin nucleic acid digestion remove device, including bottom plate (1), control box (2), chamber door (3), hole (4) charge, switch (5), operation panel (6), operating panel (7), louvre (8), regulating box (9), bacterin liquid injection tube (10), PBS buffer solution injection tube (11), driving motor (12), pivot (13), micro-filtration hollow fiber membrane (14), first stirring leaf (15), second stirring leaf (16), solenoid valve (17), ultrafiltration hollow fiber membrane (18), pH test head (19), salt concentration test head (20), pH value tester (21), salt concentration tester (22), first filling opening (23), second filling opening (24), drain pump (25), treatment tank (26), baffle (27), power (28), spring (29), shock attenuation cotton (30), electronic box (31), An exchange chamber (32), an inactivation chamber (33), an enzyme treatment chamber (34), a purification chamber (35), an anion exchange medium port (36), an inactivator port (37), a heating wire (38), a restriction endonuclease port (39), a gel filtration column (40), and a storage chamber (41), characterized in that: the device is characterized in that an operation box (2) is arranged at one end of the top of the bottom plate (1), a partition plate (27) is symmetrically welded in the middle of the inner wall of the operation box (2), a power supply (28) and an electric box (31) are respectively arranged on the partition plate (27), springs (29) are symmetrically arranged at the bottom of the inner wall of the operation box (2), damping cotton (30) is filled in gaps between the springs (29) and the partition plate (27), an adjusting box (9) is arranged on one side of the operation box (2), a pH value tester (21) and a salt concentration tester (22) are respectively installed on one side of the adjusting box (9), a first injection port (23) and a second injection port (24) are respectively formed in one corresponding end of the middle of the surface of the adjusting box (9), a vaccine solution injection pipe (10) and a PBS buffer solution injection pipe (11) are respectively welded in the corresponding two ends of one side of the top of the adjusting box (9), the welding of the inner wall top of regulating box (9) has driving motor (12), the output of driving motor (12) is through pivot (13) respectively with first stirring leaf (15) and second stirring leaf (16) fixed connection, first stirring leaf (15) top is provided with micro-filtration hollow fiber membrane (14), and PBS buffer solution injection pipe (11) bottom one end stretches into micro-filtration hollow fiber membrane (14) below, be provided with solenoid valve (17) in the middle of the inner wall of regulating box (9), ultrafiltration hollow fiber membrane (18) are installed to the below of solenoid valve (17), pH test head (19) and salt concentration test head (20) are installed respectively to the corresponding both ends in inner wall bottom one side of regulating box (9), regulating box (9) opposite side is passed through drain pump (25) and is connected with treatment tank (26), treatment tank (26) inner wall top is provided with swap chamber (32), an anion exchange medium port (36) is formed in one side of the exchange chamber (32), an inactivation chamber (33) is arranged at the bottom of the exchange chamber (32), an inactivating agent port (37) is formed in one side of the inactivation chamber (33), an enzyme treatment chamber (34) is arranged at the bottom of the inactivation chamber (33), a restriction endonuclease port (39) is formed in one side of the enzyme treatment chamber (34), heating wires (38) are mounted at two corresponding ends of one middle side of the enzyme treatment chamber (34), a purification chamber (35) is arranged at the bottom of the enzyme treatment chamber (34), a gel filter column (40) is arranged at the middle of the purification chamber (35), and a storage chamber (41) is arranged at the bottom of the purification chamber (35); the aperture of the microfiltration hollow fiber membrane (14) is 0.45 mu m, and the aperture of the ultrafiltration hollow fiber membrane (18) is 300 KD.
2. The rabies vaccine nucleic acid digestion removal device according to claim 1, wherein: one side of the surface of the operation box (2) is hinged with a box door (3) through a hinge, and one side of the surface of the box door (3) is welded with a handle.
3. The nucleic acid digestion removal device for rabies vaccines according to claim 2, wherein: charging hole (4) have been seted up to surface one side top one end of control box (2), switch (5) are installed to the surface one side top other end of control box (2), the top of control box (2) is provided with operation panel (6), operation panel (6) surface one side is provided with operating panel (7).
4. The nucleic acid digestion removal device for rabies vaccines according to claim 3, wherein: the bottom of one side of the surface of the operation box (2) is uniformly provided with heat dissipation holes (8).
5. An experimental method of the rabies vaccine nucleic acid digestion removal device according to any one of claims 1 to 4, comprising the steps of firstly, filtering and concentrating vaccine liquid; adjusting the pH value and the salt concentration; step three, anion exchange; step four, inactivation; step five, enzyme treatment; step six, purification; the method is characterized in that:
in the first step, vaccine liquid is introduced into a regulating box (9) through a vaccine liquid injection pipe (10), the vaccine liquid is subjected to primary clarification and filtration through a microfiltration hollow fiber membrane (14) to form filtrate, a PBS buffer liquid is injected into the filtrate through a PBS buffer liquid injection pipe (11) to form a mixed liquid, a switch (5) is turned on, a driving motor (12) works, a first stirring blade (15) and a second stirring blade (16) are driven to rotate through a rotating shaft (13), the mixed liquid is fully stirred, an electromagnetic valve (17) is turned on, the mixed liquid is subjected to secondary clarification and filtration through an ultrafiltration hollow fiber membrane (18), and the treated vaccine liquid is obtained; the aperture of the microfiltration hollow fiber membrane (14) is 0.45 mu m, and the aperture of the ultrafiltration hollow fiber membrane (18) is 300 KD;
in the second step, a pH value tester (21) and a salt concentration tester (22) work, the pH value and the salt concentration of the treated vaccine liquid are tested through a pH test head (19) and a salt concentration test head (20), hydrochloric acid and a sodium hydroxide solution are added into an adjusting box (9) through a first injection port (23) to adjust the pH value of the vaccine liquid, and a sodium chloride solution is added into the adjusting box (9) through a second injection port (24) to adjust the salt concentration of the vaccine liquid; adjusting the pH value of the vaccine liquid to be 6-8 and the salt concentration to be 0.2-0.5 mol/mol;
in the third step, the vaccine liquid with the adjusted pH value and salt concentration is introduced into an exchange chamber (32) of a treatment tank (26) through a liquid guide pump (25), and an anion exchange medium is injected into the exchange chamber (32) through an anion exchange medium port (36) to carry out anion exchange treatment;
in the fourth step, the vaccine liquid after anion exchange treatment enters an inactivation chamber (33), an inactivator is injected into the inactivation chamber (33) through an inactivator port (37) for inactivation for 24-36 h, and the pH value of an inactivation system is controlled to be stabilized between 7.0-7.4 in the inactivation process; the inactivator is beta-propiolactone, and accounts for 0.05-0.2% of the volume of the vaccine liquid;
in the fifth step, the inactivated vaccine liquid enters an enzyme treatment chamber (34), restriction endonuclease is injected into the enzyme treatment chamber (34) through a restriction endonuclease port (39) to digest nucleic acid, a heating wire (38) works, the temperature of the vaccine liquid is adjusted to be between 30 and 37 ℃, and the vaccine liquid is kept stand for 24 hours;
in the sixth step, the vaccine liquid after standing enters a purification chamber (35), gel filtration treatment is carried out through a gel filtration column (40), nucleic acid is further removed, and the vaccine liquid after removal enters a storage chamber (41) for storage.
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