CN111972669A - Probiotic powder for enhancing immunity and preparation method thereof - Google Patents

Probiotic powder for enhancing immunity and preparation method thereof Download PDF

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Publication number
CN111972669A
CN111972669A CN202010802088.4A CN202010802088A CN111972669A CN 111972669 A CN111972669 A CN 111972669A CN 202010802088 A CN202010802088 A CN 202010802088A CN 111972669 A CN111972669 A CN 111972669A
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powder
freeze
probiotic
probiotics
dried powder
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刘敏
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Chengdu Miertang Vocational Skills Training School Co ltd
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Chengdu Miertang Vocational Skills Training School Co ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/03—Organic compounds
    • A23L29/035—Organic compounds containing oxygen as heteroatom
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/03—Organic compounds
    • A23L29/045—Organic compounds containing nitrogen as heteroatom
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
    • A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P10/00—Shaping or working of foodstuffs characterised by the products
    • A23P10/30—Encapsulation of particles, e.g. foodstuff additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/113—Acidophilus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/11—Lactobacillus
    • A23V2400/137—Delbrueckii
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/21—Streptococcus, lactococcus
    • A23V2400/249—Thermophilus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/513—Adolescentes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/531—Lactis
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00—Lactic or propionic acid bacteria
    • A23V2400/51—Bifidobacterium
    • A23V2400/533—Longum

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  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
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Abstract

The invention discloses probiotic powder for enhancing immunity and a preparation method thereof, and relates to the technical field of health-care food, wherein the probiotic powder comprises probiotics, prebiotics and an encapsulating layer, and the probiotics comprise bifidobacterium longum freeze-dried powder, bifidobacterium lactis freeze-dried powder, bifidobacterium adolescentis freeze-dried powder, streptococcus thermophilus freeze-dried powder, lactobacillus acidophilus freeze-dried powder and lactobacillus delbrueckii subsp bulgaricus freeze-dried powder; the invention solves the problem that the esophagus and other environments have influence on the activity of the probiotics after the probiotics powder is taken, and the embedded layer provides protection for unstable probiotics; the prebiotics have good biocompatibility, the embedding yield and efficiency are improved, the acid resistance and bile resistance of the prebiotic powder are improved, the mutual synergistic effect of the components of the embedding layer and the prebiotics is realized, the embedding yield and efficiency are improved, the tolerance to gastric acid is also improved, the low-pH gastric acid corrosion is resisted, the damage by bile is avoided, and the targeted planting of the prebiotics is ensured.

Description

Probiotic powder for enhancing immunity and preparation method thereof
Technical Field
The invention relates to the technical field of health-care food, in particular to probiotic powder for enhancing immunity and a preparation method thereof.
Background
Probiotics (Probiotics), also known as viable bacteria preparation, microbial regulator, ecological product, growth-promoting agent and the like, is an active microorganism which can promote the ecological balance of the intestinal microbial flora of a host and has beneficial effect on the host. Probiotics are widely distributed in nature, can colonize the intestinal tract and other organs of humans and animals, and are found in river water, lakes, feed, soil, food, plant rhizosphere and some clinical samples. The probiotics mainly comprise lactobacillus, bacillus, yeast, bifidobacterium, gram-positive coccus, some escherichia coli, photosynthetic bacteria and the like. The probiotics have been developed for years from discovery to clinical application, and the physiological effects of the probiotics are gradually clear, which mainly show that the probiotics can inhibit the growth of harmful bacteria in the intestinal tract of a host, can regulate the micro-ecological balance of the intestinal tract, and simultaneously have the capabilities of reducing cholesterol in the organism and promoting the immune function of the organism.
It is important to note that the probiotics produce a corresponding effect in the host only when the probiotics reach a certain quantitative level. Therefore, foods, health products or medicines containing probiotics as functional ingredients need to ensure that the number of live probiotics contained is kept at a certain level. The requirement provides corresponding requirements for the production and preparation of probiotics, and in the prior art, the method for activating and culturing the probiotics is more traditional, and the obtained seed liquid has low activity, so that the application effect is poor. Because the storage conditions of the probiotics are harsh, the existing products generally have the technical problems that viable bacteria are easy to die, the number of the viable bacteria is unstable, the shelf life of the probiotic products is short, and the quality of the probiotic products is unstable.
Healthy intestinal flora colonization plays an important role in the development of early immunity and the establishment of intestinal barrier function, and is helpful for protecting babies against the threat of infection and allergy. The place where the probiotics finally act is in the lower end of the digestive tract, i.e. the middle and lower small intestine and the large intestine, and therefore whether the probiotics are able to reach the destination, on the intestinal wall, against the various digestive fluids of the digestive tract depends on their "adhesion" and "colonization" capabilities. The existing probiotic products have poor target colonization ability and lose activity when not reaching a target position.
The existing probiotic strains generally exist in the form of freeze-dried powder, when the probiotic freeze-dried powder is processed again, technological operations such as rehydration, wetting, stirring with other materials, mixing and the like of the freeze-dried powder are inevitably involved, when the operations are carried out, the activity of the probiotic strains is damaged, and the viable count of the probiotics is reduced to different degrees.
In conclusion, a probiotic powder with higher stability and capable of enhancing immunity is yet to be researched.
Disclosure of Invention
The invention aims to solve the problems in the prior art and provides probiotic powder for enhancing immunity and a preparation method thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
the probiotic powder comprises probiotics, prebiotics and an encapsulating layer, wherein the probiotics comprise bifidobacterium longum freeze-dried powder, bifidobacterium lactis freeze-dried powder, bifidobacterium adolescentis freeze-dried powder, streptococcus thermophilus freeze-dried powder, lactobacillus acidophilus freeze-dried powder and lactobacillus delbrueckii subsp bulgaricus freeze-dried powder.
Preferably, the prebiotic comprises stachyose and inulin.
Preferably, the mass ratio of the bifidobacterium longum freeze-dried powder, the bifidobacterium lactis freeze-dried powder, the bifidobacterium adolescentis freeze-dried powder, the streptococcus thermophilus freeze-dried powder, the lactobacillus acidophilus freeze-dried powder and the lactobacillus delbrueckii subsp bulgaricus freeze-dried powder is 1-2: 1-3: 2-3: 1-2: 2-4: 1-2.
Preferably, the mass ratio of stachyose to inulin is 1-2: 2-3, wherein the mass ratio of the stachyose to the bifidobacterium longum freeze-dried powder is 5-6: 1-2.
Preferably, the coating layer comprises poly-L-arginine and chitosan.
Preferably, the mass ratio of the poly-L-arginine to the chitosan is 1: 1-4, wherein the mass ratio of the chitosan to the bifidobacterium longum freeze-dried powder is 8-12: 2-4.
Preferably, the amount of the live bacteria in the probiotic powder is 109-1010CFU/g。
Preferably, the composite probiotics is powder.
Preferably, the preparation method of the probiotic powder for enhancing immunity comprises the following steps:
s1, mixing lyophilized powder of Bifidobacterium longum, lyophilized powder of Bifidobacterium lactis, lyophilized powder of Bifidobacterium adolescentis, lyophilized powder of Streptococcus thermophilus, lyophilized powder of Lactobacillus acidophilus and lyophilized powder of Lactobacillus delbrueckii subsp bulgaricus uniformly to obtain probiotic;
s2, sequentially adding prebiotics and embedding layers into the probiotics, and uniformly mixing to obtain a mixture I;
s3, freeze-drying the mixture I to obtain the probiotic powder for enhancing the immunity.
The bifidobacterium longum is mainly planted at the lower end of the intestinal tract, needs a weak acid environment for growth, and can enter a growth stable period within 48 hours. The specific fructose-6-phosphoketolase decomposes glucose into acetic acid and lactic acid, lowers the pH value of intestinal tracts, and inhibits the growth and reproduction of foreign bacteria. Research shows that short-chain fatty acids such as acetic acid and propionic acid produced by Bifidobacterium longum have antibacterial activity and have antibacterial effect on Pseudomonas and Staphylococcus aureus. Thereby inhibiting the growth of harmful bacteria and pathogenic bacteria in the intestinal tract. And the bifidobacterium belongs to obligate anaerobe, and the growth speed of the bifidobacterium is higher than that of facultative anaerobe under the anaerobic condition, so that the obligate anaerobe can grow preferentially under the condition of limited nutrient substances, and the potential pathogenic bacteria of the facultative anaerobe are in a disadvantage state generally. As intestinal dominant bacteria, the probiotic bacteria have remarkable probiotic functions, can be tightly planted on intestinal mucosa, and play the functions of nourishing the intestinal tract, blocking harmful bacteria planting, regulating immunity, balancing flora and repairing damaged intestinal barriers.
The lactobacillus in the Bulgaria yoghourt belongs to lactobacillus in classification, and is named as Lactobacillus delbrueckii Bulgaria subspecies (Lactobacillus bulgaria for short) by microbiologists due to the characteristics of excellent strain producing area, microbial characteristics, efficiency and the like. Excellent efficiency and can help people to have a good health and a long life. Can ferment sugar to obtain energy and produce a large amount of lactic acid.
Bifidobacterium adolescentis is a probiotic, and can be used for treating chronic diarrhea and constipation, and also has antiaging effect.
Streptococcus thermophilus can only reach the upper half of the small intestine, not the large intestine as does Bifidobacterium. Scientists believe that the live yoghurt bacteria streptococcus thermophilus can help lactose intolerant people digest lactose because this bacteria can produce lactase.
Lactobacillus acidophilus belongs to the genus Lactobacillus, gram-positive bacilli, the ends of the rods are rounded, and are mainly present in the small intestine, releasing lactic acid, acetic acid and some antibiotics acting on harmful bacteria, but the beneficial bacteria action is weak. Regulating intestinal flora balance, and inhibiting proliferation of intestinal undesirable microorganisms. Lactobacillus acidophilus has antagonistic effect on pathogenic microorganisms. The intake of the food containing the live bacteria is beneficial to digestive organs, especially for infants with gastrointestinal dysfunction and some patients with gastrointestinal dysfunction caused by long-term oral administration of antibiotics, and can quickly restore normal balance of flora in intestinal tracts and inhibit proliferation of putrefying bacteria, so the food has good nutrition and health care effects.
After a user swallows the strain, the strain passes through a plurality of organs from the oral cavity to the intestines and stomach, acidic substances in the esophagus and the stomach can destroy the activity of the strain in a large amount, so that few active strains finally entering the intestinal tract are remained, and the improvement of the targeted colonization effect of the strain is vital.
The strain combination of the scheme forms a combined flora which can grow fast and has lasting effect under different conditions, and a biological barrier is formed on the surface of the whole intestinal mucosa to inhibit harmful bacteria in the intestines, so that the intestinal function is improved. In addition, the live bifidobacterium duplex bacteria can produce lactic acid and acetic acid after fermentation in human intestines, can improve the utilization rate of calcium, phosphorus and iron, promote the absorption of iron and vitamin D, produce vitamins B1, B2, B6 and B12 and essential nutrients of human bodies such as alanine, valine, aspartic acid, threonine and the like, and have important nutrition effect which is not ignored for human bodies.
Stachyose is a naturally occurring tetrasaccharide, and is a functional oligosaccharide which can obviously promote the proliferation of beneficial bacteria such as bifidobacterium and the like. The pure product is white powder, has slightly sweet taste, sweetness of 22 percent of that of the cane sugar, pure taste and no bad taste or peculiar smell.
Inulin is a reserve polysaccharide in plants, mainly comes from plants, and has been found in 36000 kinds, including 11 families of dicotyledonous plants such as Compositae, Campanulaceae and Gentianaceae, and 11 families of monocotyledonous plants such as Liliaceae and gramineae. For example, inulin is abundant in Jerusalem artichoke, tubers of chicory, root tuber of Paeonia suffruticosa (Dali chrysanthemum), and roots of Cirsium japonicum, wherein inulin content of Jerusalem artichoke is the highest.
The poly-L-arginine and the chitosan can improve the biocompatibility of the microcapsule, further improve the positioning and releasing capacity of the probiotics, further keep the stability, the activity and the effective planting rate of the composition to the maximum extent, and effectively prevent the reduction of the activity of the probiotics in the using and storing processes.
Therefore, the combination of the probiotic strain species in the scheme is a combined flora which can grow fast and has lasting effect, a biological barrier is formed on the surface of the whole intestinal mucosa, and harmful bacteria in the intestines are inhibited, so that the intestinal function is improved. The process of combining the probiotic composition with the epithelial cells of the host intestine in the intestinal tract is firstly the specificity of the host, and bacteria from a human body cannot necessarily be colonized in an animal body, and bacteria from the animal also cannot necessarily be colonized in the human body; secondly, the specificity of the bacterial strains, some bacterial strains of the same species can be attached and planted, and some bacterial strains can not be attached and planted; in addition, enough quantity is needed, the adhesion has colony effect, and the adhesion can be firm only when the bacteria reach a certain quantity; therefore, the probiotic strains of the scheme achieve the technical effect of combining the probiotic strains with the intestinal epithelial cells of the host whether the strains are combined by the strains or the strains are combined. Meanwhile, adhesion can prevent probiotics from being cleared by liquid secreted by gastrointestinal tracts such as gastric acid, bile and the like and intestinal peristalsis, and the possibility of adhesion of other harmful bacteria is reduced by the adhered bacteria. Meanwhile, a biological barrier is formed to prevent harmful bacteria from approaching epithelial cells of the intestinal tract. The adhered bacterial strains are fixedly planted to form a microbial film on the surface of the adhered film, so that the invasion of exogenous bacteria is prevented, and the health of intestinal mucosa is protected. Lactobacillus acidophilus, Bifidobacterium and the like have strong adhesion, colonization and adaptability to the digestive tract environment.
Meanwhile, auxiliary components such as prebiotics and embedding layers are added, and the components are coated, so that the activity of most of probiotic strains is kept compared with that of probiotics directly taken in the prior art when the probiotic strain composition is eaten in the process of entering the intestinal tract of a human body, and then the subsequent adhesion of the probiotic strains on epithelial cells of a host intestine is facilitated, and the targeted planting rate of the probiotic strains is improved.
Compared with the prior art, the invention provides the probiotic powder for enhancing the immunity and the preparation method thereof, and the probiotic powder has the following beneficial effects:
1. the prebiotics and the embedding layer can improve the biocompatibility of the microcapsule, improve the targeting planting effect of the probiotics, maintain the stability, activity and effective planting rate of the composition, and effectively prevent the reduction of the activity of the probiotics in the using and storing processes;
2. the prebiotics in the invention are helpful for coordinating intestines and stomach, can directly reach the intestines, are not corroded and damaged by acid gastric juice, generate volatile fatty acid, reduce the pH value of the intestines, directly promote and proliferate probiotic groups, indirectly inhibit the growth of harmful bacteria and protect the microecology of the intestines;
3. the invention solves the problem that the esophagus and other environments have influence on the activity of the probiotics after the probiotics powder is taken, and the embedded layer provides protection for unstable probiotics; the prebiotics have good biocompatibility, the embedding yield and efficiency of the prebiotics are improved, the acid resistance and bile resistance of the probiotic powder are improved, the embedding layer and the prebiotics play a mutual synergistic effect, the embedding yield and efficiency are improved, the gastric acid resistance is also improved, the low-pH gastric acid erosion is resisted, the damage by bile is avoided, and the targeted planting of the prebiotics is ensured;
4. the probiotic combination is specially treated, so that the activity of probiotics is improved, the balance of intestinal flora is effectively adjusted, and the immunity of the organism is improved;
5. the preparation method has simple process, reduces the production cost, and ensures that good income can be obtained in industrialization; the preparation method of the invention has high embedding efficiency and good embedding effect on the probiotics.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Example 1:
a probiotic powder for enhancing immunity and a preparation method thereof comprise the following steps:
s1, preparation of thalli: weighing probiotics, bifidobacterium longum freeze-dried powder, bifidobacterium lactis freeze-dried powder, bifidobacterium adolescentis freeze-dried powder, streptococcus thermophilus freeze-dried powder, lactobacillus acidophilus freeze-dried powder and lactobacillus delbrueckii subsp bulgaricus freeze-dried powder according to the weight ratio of 1: 1: 2: 1: 2: 1, uniformly mixing to prepare the probiotics, wherein the amount of the live bacteria in the probiotic powder is 109-1010CFU/g；
S2, adding prebiotics and an embedding layer into probiotics to obtain a mixture I, wherein the mass ratio of stachyose to inulin is 1: 2, the mass ratio of the stachyose to the bifidobacterium longum freeze-dried powder is 5: 1; the mass ratio of the poly-L-arginine to the chitosan is 1: 1; the mass ratio of the chitosan to the bifidobacterium longum freeze-dried powder is 8: 2;
s3, freeze-drying to obtain the probiotic powder for enhancing immunity.
Example 2:
a probiotic powder for enhancing immunity and a preparation method thereof comprise the following steps:
s1, preparation of thalli: weighing probiotics, bifidobacterium longum freeze-dried powder, bifidobacterium lactis freeze-dried powder, bifidobacterium adolescentis freeze-dried powder, streptococcus thermophilus freeze-dried powder, lactobacillus acidophilus freeze-dried powder and lactobacillus delbrueckii subsp bulgaricus freeze-dried powder according to the weight ratio of 2: 3: 3: 2: 4: 2, uniformly mixing to prepare the probiotics, wherein the amount of the live bacteria in the probiotic powder is 109-1010CFU/g；
S2, adding prebiotics and an embedding layer into probiotics to obtain a mixture I, wherein the mass ratio of stachyose to inulin is 2: 3, the mass ratio of the stachyose to the bifidobacterium longum freeze-dried powder is 6: 2; the mass ratio of the poly-L-arginine to the chitosan is 1: 4; the mass ratio of the chitosan to the bifidobacterium longum freeze-dried powder is 12: 4;
s3, freeze-drying to obtain the probiotic powder for enhancing immunity.
Example 3:
a probiotic powder for enhancing immunity and a preparation method thereof comprise the following steps:
s1, preparation of thalli: weighing probiotics, bifidobacterium longum freeze-dried powder, bifidobacterium lactis freeze-dried powder, bifidobacterium adolescentis freeze-dried powder, streptococcus thermophilus freeze-dried powder, lactobacillus acidophilus freeze-dried powder and lactobacillus delbrueckii subsp bulgaricus freeze-dried powder according to the proportion, wherein the mass ratio of the probiotics to the bifidobacterium longum freeze-dried powder to the bifidobacterium lactis freeze-dried powder to the lactobacillus delbrueckii subsp bu: 2: 2.5: 1.5: 3: 1.5, uniformly mixing to prepare the probiotics, wherein the amount of the live bacteria in the probiotic powder is 109-1010CFU/g；
S2, adding prebiotics and an embedding layer into probiotics to obtain a mixture I, wherein the mass ratio of stachyose to inulin is 1.5: 2.5, the mass ratio of the stachyose to the bifidobacterium longum freeze-dried powder is 5.5: 1.5; the mass ratio of the poly-L-arginine to the chitosan is 1: 2.5; the mass ratio of the chitosan to the bifidobacterium longum freeze-dried powder is 10: 3;
s3, freeze-drying to obtain the probiotic powder for enhancing immunity.
Example 4:
a probiotic powder for enhancing immunity and a preparation method thereof comprise the following steps:
s1, preparation of thalli: weighing probiotics, bifidobacterium longum freeze-dried powder, bifidobacterium lactis freeze-dried powder, bifidobacterium adolescentis freeze-dried powder, streptococcus thermophilus freeze-dried powder, lactobacillus acidophilus freeze-dried powder and lactobacillus delbrueckii subsp bulgaricus freeze-dried powder according to the weight ratio of 1: 3: 2: 2: 2: 2, uniformly mixing to prepare the probiotics, wherein the amount of the live bacteria in the probiotic powder is 109-1010CFU/g；
S2, adding prebiotics and an embedding layer into probiotics to obtain a mixture I, wherein the mass ratio of stachyose to inulin is 1: 3, the mass ratio of the stachyose to the bifidobacterium longum freeze-dried powder is 5: 2; the mass ratio of the poly-L-arginine to the chitosan is 1: 2; the mass ratio of the chitosan to the bifidobacterium longum freeze-dried powder is 8: 4;
s3, freeze-drying to obtain the probiotic powder for enhancing immunity.
Example 5:
a probiotic powder for enhancing immunity and a preparation method thereof comprise the following steps:
s1, preparation of thalli: weighing probiotics, bifidobacterium longum freeze-dried powder, bifidobacterium lactis freeze-dried powder, bifidobacterium adolescentis freeze-dried powder, streptococcus thermophilus freeze-dried powder, lactobacillus acidophilus freeze-dried powder and lactobacillus delbrueckii subsp bulgaricus freeze-dried powder according to the weight ratio of 2: 1: 3: 1: 4: 1, uniformly mixing to prepare the probiotics, wherein the amount of the live bacteria in the probiotic powder is 109-1010CFU/g；
S2, adding prebiotics and an embedding layer into probiotics to obtain a mixture I, wherein the mass ratio of stachyose to inulin is 2: 2, the mass ratio of the stachyose to the bifidobacterium longum freeze-dried powder is 5: 2; the mass ratio of the poly-L-arginine to the chitosan is 1: 3; the mass ratio of the chitosan to the bifidobacterium longum freeze-dried powder is 12: 2;
s3, freeze-drying to obtain the probiotic powder for enhancing immunity.
Comparative example 1:
in the comparative example 1, no prebiotics are added to the probiotic powder, and the strains are simply mixed and then embedded; the rest of the steps are consistent with example 1.
Comparative example 2:
the probiotic powder in the comparative example 2 is not added with an embedding layer, and the strain and the body benefiting essence are simply mixed; the rest of the steps are consistent with example 1.
Comparative example 3:
the probiotic powder in the comparative example 3 is not added with prebiotics and embedding layers, and the strains are simply mixed; the rest of the steps are consistent with example 1.
The prepared probiotic powder for enhancing immunity of the above examples 1 to 5 and comparative examples 1 to 3 were subjected to a viable bacteria content test.
The results are shown in table 1 below:
Figure BDA0002627765050000071
Figure BDA0002627765050000081
referring to the above examples 1-5, it can be seen from the experimental data in the comparative examples 1-3 and table 1 that, compared with the comparative examples 1-3, the content of viable bacteria in the probiotic powder for enhancing immunity in the present invention is higher, thereby solving the problem of the influence of the environment such as the esophagus after taking the probiotic powder on the activity of the probiotic, and the embedding layer of the present invention provides protection for unstable probiotic; the prebiotics have good biocompatibility, the embedding yield and efficiency of the prebiotics are improved, the acid resistance and bile resistance of the probiotic powder are improved, the embedding layer and the prebiotics play a mutual synergistic effect, the embedding yield and efficiency are improved, the gastric acid resistance is also improved, the low-pH gastric acid erosion is resisted, the damage by bile is avoided, and the targeted planting of the prebiotics is ensured; the prebiotics and the embedding layer can improve the biocompatibility of the microcapsule, improve the targeting planting effect of the probiotics, maintain the stability, activity and effective planting rate of the composition, and effectively prevent the reduction of the activity of the probiotics in the using and storing processes; the prebiotics in the invention are helpful for coordinating intestines and stomach, can directly reach the intestines, are not corroded and damaged by acid gastric juice, generate volatile fatty acid, reduce the pH value of the intestines, directly promote and proliferate probiotic groups, indirectly inhibit the growth of harmful bacteria and protect the microecology of the intestines; the probiotic combination is specially treated, so that the activity of the probiotics is improved, the balance of intestinal flora is effectively adjusted, and the immunity of the organism is improved. The preparation method has simple process, reduces the production cost, and ensures that good income can be obtained in industrialization; the preparation method of the invention has high embedding efficiency and good embedding effect on the probiotics.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (8)

1. The probiotic powder is characterized by comprising probiotics, prebiotics and an encapsulating layer, wherein the probiotics comprise bifidobacterium longum freeze-dried powder, bifidobacterium lactis freeze-dried powder, bifidobacterium adolescentis freeze-dried powder, streptococcus thermophilus freeze-dried powder, lactobacillus acidophilus freeze-dried powder and lactobacillus delbrueckii subsp bulgaricus freeze-dried powder.
2. The probiotic powder for enhancing immunity according to claim 1, wherein the mass ratio of the bifidobacterium longum lyophilized powder, the bifidobacterium lactis lyophilized powder, the bifidobacterium adolescentis lyophilized powder, the streptococcus thermophilus lyophilized powder, the lactobacillus acidophilus lyophilized powder and the lactobacillus delbrueckii subsp bulgaricus lyophilized powder is 1-2: 1-3: 2-3: 1-2: 2-4: 1-2.
3. The probiotic powder for boosting immunity according to claim 1, wherein the prebiotics comprise stachyose and inulin.
4. The probiotic powder for enhancing immunity according to claim 3, wherein the mass ratio of stachyose to inulin is 1-2: 2-3, wherein the mass ratio of the stachyose to the bifidobacterium longum freeze-dried powder is 5-6: 1-2.
5. The probiotic powder for boosting immunity according to claim 1, characterized in that the encapsulating layer comprises poly-L-arginine and chitosan.
6. The probiotic powder for enhancing immunity according to claim 5, wherein the mass ratio of poly-L-arginine to chitosan is 1: 1-4, wherein the mass ratio of the chitosan to the bifidobacterium longum freeze-dried powder is 8-12: 2-4.
7. The probiotic powder for enhancing immunity according to claim 1, characterized in that the amount of live bacteria in the probiotic powder is 109-1010CFU/g。
8. The method for preparing the probiotic powder for enhancing immunity according to any one of claims 1 to 7, characterized by comprising the following steps:
s1, mixing lyophilized powder of Bifidobacterium longum, lyophilized powder of Bifidobacterium lactis, lyophilized powder of Bifidobacterium adolescentis, lyophilized powder of Streptococcus thermophilus, lyophilized powder of Lactobacillus acidophilus and lyophilized powder of Lactobacillus delbrueckii subsp bulgaricus uniformly to obtain probiotic;
s2, sequentially adding prebiotics and embedding layers into the probiotics, and uniformly mixing to obtain a mixture I;
s3, freeze-drying the mixture I to obtain the probiotic powder for enhancing the immunity.
CN202010802088.4A 2020-08-11 2020-08-11 Probiotic powder for enhancing immunity and preparation method thereof Withdrawn CN111972669A (en)

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