CN111965309A - Portable plant photosynthetic rate detector and method thereof - Google Patents

Portable plant photosynthetic rate detector and method thereof Download PDF

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Publication number
CN111965309A
CN111965309A CN202010952677.0A CN202010952677A CN111965309A CN 111965309 A CN111965309 A CN 111965309A CN 202010952677 A CN202010952677 A CN 202010952677A CN 111965309 A CN111965309 A CN 111965309A
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China
Prior art keywords
chamber
blade
electromagnetic valve
leaf
way electromagnetic
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CN202010952677.0A
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Chinese (zh)
Inventor
杨春勇
刘剑
邓芳
侯金
陈少平
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South Central Minzu University
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South Central University for Nationalities
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/0098Plants or trees
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N7/00Analysing materials by measuring the pressure or volume of a gas or vapour

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  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
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  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention discloses a portable plant photosynthetic rate detector and a method thereof, and relates to the field of plant physiological function detection. The detector comprises a blade (00) which is provided with a blade chamber (10), a differential gas circuit unit (20), a power supply (30), a sensor array (40) and a micro-control platform (50); the blade (00) is arranged in the blade chamber (10), the blade chamber (10) is communicated with the differential gas circuit unit (20), a sensor array (40) is arranged in a measuring chamber (23) of the differential gas circuit unit (20), and the sensor array (40) is connected with the micro-control platform (50); a power supply (30) provides power to the sensor array (40) and the micro-control platform (50). Compared with the prior art, the invention has the following advantages and positive effects: can quickly, accurately and real-timely determine the different CO of plants2Photosynthetic rate under environmental conditions of concentration, illumination intensity and temperature and humidity; secondly, the structure is light and handy, the carrying is convenient, and the application range is wide; support ofThe outdoor use.

Description

Portable plant photosynthetic rate detector and method thereof
Technical Field
The invention relates to the field of plant physiological function detection, in particular to a portable plant photosynthetic rate detector and a method thereof.
Background
Photosynthesis is an important characteristic of growth and development of green plants, measurement of the photosynthetic rate of the plants is an important index for judging physiological characteristics of the plants, and the photosynthetic rate of the plants can be used as data for judging a suitable living environment of the plants.
When plants undergo photosynthesis, CO is absorbed from the environment2And water, which generates carbohydrates and releases oxygen. Illumination intensity and CO in the environment when plants are subjected to photosynthesis2Concentration is a main factor influencing the plant photosynthetic rate, and the temperature and humidity of the environment also influence the plant photosynthetic rate.
The traditional method for measuring the photosynthetic rate of plants is as follows:
1. the improved semi-leaf method needs to separate leaves from plants and then measure, has complex operation and large experimental error, and cannot measure the photosynthetic rate of the plants in real time.
2. The gas volume change method can only measure a few small plants, and has a limited application range.
3. Infrared CO2The analysis method is influenced by a measurement field and cannot measure the photosynthetic rate of outdoor plants.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a portable plant photosynthetic rate detector and a method thereof, which can rapidly, accurately and real-timely detect different CO of plants2Photosynthetic rate under ambient conditions of concentration, illumination intensity and temperature and humidity.
The purpose of the invention is realized as follows:
the detector comprises a blade, a blade chamber, a differential gas circuit unit, a power supply, a sensor array and a micro-control platform, wherein the blade chamber is provided with the blade chamber;
the measured blade is arranged in the blade chamber, the blade chamber is communicated with the differential gas circuit unit, a sensor array is arranged in the measuring chamber of the differential gas circuit unit, and the sensor array is connected with the micro control platform;
the power supply provides power to the sensor array and the micro-control platform.
Compared with the prior art, the invention has the following advantages and positive effects:
can quickly, accurately and real-timely determine the different CO of plants2Photosynthetic rate under environmental conditions of concentration, illumination intensity and temperature and humidity;
secondly, the structure is light and handy, the carrying is convenient, and the application range is wide;
and thirdly, outdoor use is supported.
Drawings
FIG. 1 is a schematic view of the present detector;
fig. 2 is a schematic structural view of a leaf chamber.
In the figure:
00-blade;
10-the leaf chamber,
1A-upper blade chamber, 1B-lower blade chamber, 1C-screw,
11-inlet, 12-outlet;
20-a differential gas circuit unit,
21-1 st three-way electromagnetic valve, 22-2 nd three-way electromagnetic valve, 23-measuring chamber, 24-air pump;
30-a power supply;
40-an array of sensors,
41—CO2a sensor, 42-a temperature and humidity sensor, 43-a gas flow rate sensor,
44-gas pressure sensor;
50-a micro-control platform, wherein,
51-ADC sampling module, 52-pump speed regulation and control board, 53-wireless transmitting module.
Detailed Description
The invention is further illustrated by the following figures and examples.
First, detector
1. General of
As shown in fig. 1, the present invention includes a measured object, i.e., a vane 00, which is provided with a vane chamber 10, a differential gas path unit 20, a power supply 30, a sensor array 40 and a micro control platform 50;
the measured blade 00 is arranged in the blade chamber 10, the blade chamber 10 is communicated with the differential gas circuit unit 20, a sensor array 40 is arranged in the measuring chamber 23 of the differential gas circuit unit 20, and the sensor array 40 is connected with the micro-control platform 50;
the power supply 30 provides power to the sensor array 40 and the micro-control platform 50.
2. Functional component
0) Blade 00
1) Leaf chamber 10
As shown in figure 2, the leaf chamber 10 comprises an upper leaf chamber 1A, a lower leaf chamber 1B and a screw rod 1C which are connected in sequence, the upper leaf chamber 1A is provided with an air inlet 11 and an air outlet 12, uniform grids are drawn on a glass sheet of the upper leaf chamber 1A and used for measuring the leaf area when the leaf of a tested plant is too small to fill the whole leaf chamber, and the area of each small grid is 1mm2The total area of the grid is 900mm2(ii) a The lower leaf chamber 1B is connected with the screw rod 1C, the lower leaf chamber 1A is controlled to move up and down by rotating the screw rod 1C, and rubber pads are attached to the contact parts of the upper leaf chamber 1A and the lower leaf chamber 1B to optimize the air tightness of the leaf chamber 10 and protect the tested plant leaves 00 from being damaged when being clamped.
The leaf chamber 10 is further provided with a control handle, and the opening and closing of the leaf chamber 10 are controlled through the control handle.
2) Differential gas circuit unit 20
As shown in fig. 1, the differential air path unit 20 includes a 1 st three-way solenoid valve 21, a 2 nd three-way solenoid valve 22, a measurement chamber 23, and an air pump 24;
a 1 st three-way electromagnetic valve 21 and a 2 nd three-way electromagnetic valve 22 which are communicated with each other are respectively connected with the air inlet 11 and the air outlet 12 of the leaf chamber 10, and the 2 nd three-way electromagnetic valve 22, the measuring chamber 23 and the air pump 24 are sequentially communicated;
a sensor array 40 is arranged in the measurement chamber 23.
3) Power supply 30
As shown in FIG. 1, the power supply 30 is a large-capacity lithium ion rechargeable battery with an input voltage of 12.6VDC, an output voltage of 12.6-10.8 VDC and a capacity of 6800 mAh.
4) Sensor array 40
As in FIG. 1, sensor array 40 includes CO2Sensor 41, temperature and humidity sensorA vessel 42, a gas flow rate sensor 43, and a gas pressure sensor 44.
(1)CO2Sensor 41
Is a universal sensor, measures CO2The concentration of (c).
(2) Temperature and humidity sensor 42
The temperature and humidity are measured by a universal sensor.
(3) Gas flow rate sensor 43
The gas flow rate is measured for a universal sensor.
(4) Gas pressure sensor 44
Gas pressure is measured for a common sensor.
5) Micro-control platform 50
As shown in fig. 1, the micro-control platform 50 selects MSP430F149 as a CPU to process data and control other modules; embedded with an ADC sampling module 51, a pump speed control board 52 and a wireless transmission module 53.
The digital signal processed by the ADC sampling module 51 is received, the pump speed control board 52 is controlled to operate the air pump 24, and the data is transmitted to the wireless transmitting module 53.
3. Mechanism of operation
The CO2 sensor, the gas flow rate sensor, the atmospheric pressure sensor and the temperature and humidity sensor in the sensor array are connected with the ADC sampling module to detect the CO in the leaf chamber2Concentration, air flow rate, atmospheric pressure and humiture turn into the signal of telecommunication, then through AD analog-to-digital conversion, turn into the recognizable digital signal of little control platform with the signal of telecommunication, little control platform carries out analysis processes to the digital signal, and then control air pump work to generate plant photosynthetic rate curve graph on sending data to intelligent mobile device with bluetooth wireless transmitting module.
Second, detection method
Initializing a detection system:
i, turning on a power supply 30 to enable each module to work normally;
II, setting the ports a and b of the 1 st three-way electromagnetic valve 21 and the ports e and d of the 2 nd three-way electromagnetic valve 22 to be communicated, so that the air flow flows in the air path according to the arrow direction of a dotted line, namely the air flow flows in the direction of the sequential connection of the 1 st three-way electromagnetic valve 21, the 2 nd three-way electromagnetic valve 22, the measuring chamber 23 and the air pump 24;
III, enabling the sensor array 40 to work normally, transmitting detection data of each sensor into the micro control platform 40, and enabling the micro control platform 40 to regulate and control the work of the air pump 24 and control the flow rate of the gas in the differential gas circuit unit 20 according to the data detected by the atmospheric pressure sensor 43 and the gas flow rate sensor 42;
Ⅳ、CO2data measured by the sensor 41 is subjected to Kalman algorithm filtering processing and ADC (analog-to-digital conversion) by an ADC (analog-to-digital converter) sampling module 51, is uploaded to the intelligent mobile equipment by a Bluetooth wireless transmitting module 53, and if CO is measured2The concentration is basically unchanged along with the time, namely the system initialization is completed;
secondly, after the system initialization is finished, the photosynthetic rate of the tested plant is detected, and the method comprises the following steps:
pressing a button of an opening and closing control handle of the leaf chamber 10 to clamp the leaf 00, counting the number of small grids on a glass sheet of an upper leaf chamber 1A occupied by the leaves in the leaf chamber 10 if the contact area of the leaf 00 is too small to fill the whole leaf chamber 10, wherein the area of each small grid is 1mm2The area of the blade 00 is estimated, and if the blade 00 can fill the whole blade chamber 10, the area of the blade 00 can be regarded as the whole blade chamber area 1600mm2
Ii, setting the ports a and c of the 1 st three-way electromagnetic valve 21 to be communicated, the port b to be closed, the ports d and f of the 2 nd three-way electromagnetic valve 22 to be communicated and the port e to be closed, so that the airflow flows in the gas path according to the arrow direction of the solid line, namely the airflow flows in the direction in which the 1 st three-way electromagnetic valve 21, the leaf chamber 10, the 2 nd three-way electromagnetic valve 22, the measuring chamber 23 and the air pump 24 are sequentially connected;
and iii, keeping the measurement for a period of time, starting a Bluetooth function of the intelligent mobile equipment, receiving data sent by the detector through Bluetooth, and collecting data for a period of time to generate a plant photosynthetic rate curve graph.

Claims (7)

1. A portable plant photosynthetic rate detector, includes blade (00), its characterized in that:
the device is provided with a leaf chamber (10), a differential gas circuit unit (20), a power supply (30), a sensor array (40) and a micro-control platform (50);
the blade (00) is arranged in the blade chamber (10), the blade chamber (10) is communicated with the differential gas circuit unit (20), a sensor array (40) is arranged in a measuring chamber (23) of the differential gas circuit unit (20), and the sensor array (40) is connected with the micro-control platform (50);
a power supply (30) provides power to the sensor array (40) and the micro-control platform (50).
2. The portable instrument for measuring photosynthetic rate of plants of claim 1 wherein:
the leaf chamber (10) comprises an upper leaf chamber (1A), a lower leaf chamber (1B) and a screw (1C), the upper leaf chamber (1A) is provided with an air inlet (11) and an air outlet (12), uniform grids are drawn on a glass sheet of the upper leaf chamber (1A) and used for measuring the area of the leaves of a tested plant when the leaves are too small to fill the whole leaf chamber, and the area of each grid is 1mm2The total area of the grid is 900mm2(ii) a The lower leaf chamber (1B) is connected with the screw (1C), the lower leaf chamber (1A) is controlled to move up and down by rotating the screw (1C), rubber pads are attached to the contact parts of the upper leaf chamber and the lower leaf chamber (1A and 1B) to optimize the air tightness of the leaf chambers and protect the tested plant leaves (00) from being damaged when being clamped;
the leaf chamber (10) is also provided with a control handle, and the opening and closing of the leaf chamber (10) are controlled by the control handle.
3. The portable instrument for measuring photosynthetic rate of plants of claim 1 wherein:
the differential gas circuit unit (20) comprises a 1 st three-way electromagnetic valve (21), a 2 nd three-way electromagnetic valve (22), a measuring chamber (23) and a gas pump (24);
a 1 st three-way electromagnetic valve (21) and a 2 nd three-way electromagnetic valve (22) which are communicated with each other are respectively connected with an air inlet (11) and an air outlet (12) of the blade chamber (10), and the 2 nd three-way electromagnetic valve (22), the measuring chamber (23) and the air pump (24) are sequentially communicated;
a sensor array (40) is arranged in the measuring chamber (23).
4. The portable instrument for measuring photosynthetic rate of plants of claim 1 wherein:
the power supply (30) is a high-capacity lithium ion rechargeable battery, the input voltage is 12.6VDC, the output voltage is 12.6-10.8 VDC, and the capacity is 6800 mAh.
5. The portable instrument for measuring photosynthetic rate of plants of claim 1 wherein:
the sensor array (40) comprises CO2A sensor (41), a temperature and humidity sensor (42), a gas flow rate sensor (43), and a gas pressure sensor (44).
6. The portable instrument for measuring photosynthetic rate of plants of claim 1 wherein:
the micro-control platform (50) selects MSP430F149 as a CPU to process data and control other modules; embedded with ADC sampling module (51), pump speed control board (52) and wireless transmitting module (53)
And receiving the digital signal processed by the ADC sampling module (51), controlling the pump speed regulating and controlling board (52) to enable the air pump (24) to work, and transmitting data to the wireless transmitting module (53).
7. The method for detecting the photosynthetic rate of a portable plant according to claim 1, 2, 3, 4, 5 or 6, comprising the steps of:
initializing a detection system:
i, turning on a power supply to enable each module to work normally;
II, setting the ports a and b of the 1 st three-way electromagnetic valve (21) and the ports e and d of the 2 nd three-way electromagnetic valve (22) to be communicated, so that the air flow flows in the direction in which the 1 st three-way electromagnetic valve (21), the 2 nd three-way electromagnetic valve (22), the measuring chamber (23) and the air pump (24) are connected in sequence;
III, the sensor array (40) works normally and transmits the detection data of each sensor into the micro control platform (50), and the micro control platform (50) regulates and controls the work of the air pump (24) according to the data detected by the gas flow rate sensor (42) and the atmospheric pressure sensor (43) and controls the gas flow rate in the differential gas circuit unit (20);
Ⅳ、CO2data measured by the sensor (41) is subjected to Kalman algorithm filtering processing and ADC (analog-to-digital conversion) by an ADC (analog-to-digital converter) sampling module (51), and is uploaded to the intelligent mobile equipment by a Bluetooth wireless transmitting module (53), and if CO is measured2The concentration is basically unchanged along with the time, namely the system initialization is completed;
secondly, after the system initialization is finished, the photosynthetic rate of the tested plant is detected, and the method comprises the following steps:
pressing a button of an opening and closing control handle of the leaf chamber (10) to clamp the leaf (00), and counting the number of small grids on the glass sheet of the upper leaf chamber occupied by the leaf in the leaf chamber (10) if the contact area of the leaf (00) is too small to fill the whole leaf chamber (10), wherein the area of each small grid is 1mm2Estimating the area of the blade, and if the blade can fill the whole blade chamber (10), the area of the blade (00) can be regarded as the whole blade chamber area 1600mm 2;
ii, setting the ports a and c of the 1 st three-way electromagnetic valve (21) to be communicated, the port b to be closed, the ports d and f of the 2 nd three-way electromagnetic valve (22) to be communicated and the port e to be closed, so that the air flow flows in the air path according to the direction in which the 1 st three-way electromagnetic valve (21), the leaf chamber (10), the 2 nd three-way electromagnetic valve (22), the measuring chamber (23) and the air pump (24) are sequentially connected;
and iii, keeping the measurement for a period of time, starting a Bluetooth function of the intelligent mobile device, receiving data sent by the detector through Bluetooth, and generating a plant photosynthetic rate curve graph.
CN202010952677.0A 2020-09-11 2020-09-11 Portable plant photosynthetic rate detector and method thereof Pending CN111965309A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
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Application Number Priority Date Filing Date Title
CN202010952677.0A CN111965309A (en) 2020-09-11 2020-09-11 Portable plant photosynthetic rate detector and method thereof

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CN111965309A true CN111965309A (en) 2020-11-20

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113093620A (en) * 2021-04-07 2021-07-09 安徽农业大学 Distributed photosynthetic rate monitoring system
CN113418888A (en) * 2021-06-21 2021-09-21 安徽农业大学 Modularized plant photosynthesis detector
CN114814104A (en) * 2022-04-28 2022-07-29 湖南杂交水稻研究中心 Device for inducing photosynthesis of single-sided leaves
CN118566444A (en) * 2024-08-01 2024-08-30 北京复兰环保科技有限公司 Photosynthetic rate detection apparatus and method

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113093620A (en) * 2021-04-07 2021-07-09 安徽农业大学 Distributed photosynthetic rate monitoring system
CN113418888A (en) * 2021-06-21 2021-09-21 安徽农业大学 Modularized plant photosynthesis detector
CN114814104A (en) * 2022-04-28 2022-07-29 湖南杂交水稻研究中心 Device for inducing photosynthesis of single-sided leaves
WO2023208030A1 (en) * 2022-04-28 2023-11-02 湖南杂交水稻研究中心 Apparatus for inducing single-side leaf photosynthesis
CN118566444A (en) * 2024-08-01 2024-08-30 北京复兰环保科技有限公司 Photosynthetic rate detection apparatus and method

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