CN111965309A - Portable plant photosynthetic rate detector and method thereof - Google Patents
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Abstract
本发明公开了一种便携式植物光合速率检测仪及其方法,涉及植物生理机能检测领域。本检测仪包括叶片(00),设置有叶室(10)、差分气路单元(20)、电源(30)、传感器阵列(40)和微控制平台(50);叶片(00)置于叶室(10)内,叶室(10)和差分气路单元(20)连通,在差分气路单元(20)的测量室(23)内设置有传感器阵列(40),传感器阵列(40)和微控制平台(50)连接;电源(30)给传感器阵列(40)和微控制平台(50)提供能源。与现有技术相比,本发明具有下列优点和积极效果:①可以快速、精确和实时地测定植株在不同的CO2浓度、光照强度和温湿度的环境条件下的光合速率;②结构轻巧,携带方便,适用范围广;③支持户外使用。
The invention discloses a portable plant photosynthetic rate detector and a method thereof, and relates to the field of plant physiological function detection. The detector includes a blade (00), which is provided with a blade chamber (10), a differential air circuit unit (20), a power supply (30), a sensor array (40) and a micro-control platform (50); the blade (00) is placed on the blade Inside the chamber (10), the leaf chamber (10) is communicated with the differential air circuit unit (20), and a sensor array (40) is arranged in the measurement chamber (23) of the differential air circuit unit (20), the sensor array (40) and The micro-control platform (50) is connected; the power supply (30) provides energy to the sensor array (40) and the micro-control platform (50). Compared with the prior art, the present invention has the following advantages and positive effects: 1. the photosynthetic rate of plants can be measured quickly, accurately and in real time under different environmental conditions of CO 2 concentration, light intensity and temperature and humidity; 2. lightweight structure, It is easy to carry and has a wide range of applications; ③It supports outdoor use.
Description
技术领域technical field
本发明涉及植物生理机能检测领域,尤其涉及一种便携式植物光合速率检测仪及其方法。The invention relates to the field of plant physiological function detection, in particular to a portable plant photosynthetic rate detector and a method thereof.
背景技术Background technique
光合作用是绿色植物生长发育的一种重要特征,测量植物的光合速率是判断植物生理特征的一项重要指标,植物的光合速率可以作为判断植物适宜的生活环境的一项数据。Photosynthesis is an important feature of the growth and development of green plants. Measuring the photosynthetic rate of plants is an important indicator for judging the physiological characteristics of plants. The photosynthetic rate of plants can be used as a data for judging the suitable living environment of plants.
植物进行光合作用时,从环境中吸收CO2和水,生成碳水化合物并释放氧气。在植物进行光合作用时,环境中的光照强度和CO2浓度是影响植物光合速率的主要因素,还有环境的温湿度也会影响植物的光合速率。When plants perform photosynthesis, they absorb CO2 and water from the environment, produce carbohydrates and release oxygen. When plants perform photosynthesis, the light intensity and CO 2 concentration in the environment are the main factors that affect the photosynthetic rate of plants, and the temperature and humidity of the environment will also affect the photosynthetic rate of plants.
传统的测量植物的光合速率的方法有:The traditional methods of measuring the photosynthetic rate of plants are:
1、改良半叶法,该方法须将叶片与植株分离,然后测量,操作复杂,实验误差大,而且无法实时测量植物的光合速率。1. Improved half-leaf method. This method must separate the leaves from the plants, and then measure them. The operation is complicated, the experimental error is large, and the photosynthetic rate of plants cannot be measured in real time.
2、气体体积变化法,这种方法只能测量一些小型植株,适用范围有限。2. The gas volume change method, this method can only measure some small plants, and the scope of application is limited.
3、红外CO2分析法,这种方法受到测量场地影响,无法测量户外植株的光合速率。3. Infrared CO 2 analysis method, this method is affected by the measurement site and cannot measure the photosynthetic rate of outdoor plants.
发明内容SUMMARY OF THE INVENTION
本发明的目的是克服现有技术存在的缺点和不足,提供一种便携式植物光合速率检测仪及其方法,可以快速、精确和实时地测定植株在不同的CO2浓度、光照强度和温湿度的环境条件下的光合速率。The purpose of the present invention is to overcome the shortcomings and deficiencies existing in the prior art, and to provide a portable plant photosynthetic rate detector and a method thereof, which can quickly, accurately and real-time measure the changes of plants in different CO2 concentrations, light intensity and temperature and humidity. Photosynthetic rate under ambient conditions.
本发明的目的是这样实现的:The object of the present invention is achieved in this way:
本检测仪包括叶片,设置有叶室、差分气路单元、电源、传感器阵列和微控制平台;The detector includes a blade, which is provided with a blade chamber, a differential air circuit unit, a power supply, a sensor array and a micro-control platform;
被测叶片置于叶室内,叶室和差分气路单元连通,在差分气路单元的测量室内设置有传感器阵列,传感器阵列和微控制平台连接;The blade to be tested is placed in the blade chamber, the blade chamber is connected with the differential air circuit unit, a sensor array is arranged in the measurement chamber of the differential air circuit unit, and the sensor array is connected with the micro-control platform;
电源给传感器阵列和微控制平台提供能源。The power supply provides energy to the sensor array and the microcontroller platform.
与现有技术相比,本发明具有下列优点和积极效果:Compared with the prior art, the present invention has the following advantages and positive effects:
①可以快速、精确和实时地测定植株在不同的CO2浓度、光照强度和温湿度的环境条件下的光合速率;①The photosynthetic rate of plants can be measured quickly, accurately and in real time under different environmental conditions of CO 2 concentration, light intensity and temperature and humidity;
②结构轻巧,携带方便,适用范围广;②The structure is light, easy to carry, and has a wide range of applications;
③支持户外使用。③Support outdoor use.
附图说明Description of drawings
图1是本检测仪的结构示意图;Fig. 1 is the structural representation of this detector;
图2是叶室的结构示意图。Figure 2 is a schematic diagram of the structure of the leaf chamber.
图中:In the picture:
00—叶片;00—leaf;
10—叶室,10—leaf chamber,
1A—上叶室,1B—下叶室,1C—螺杆,1A—upper chamber, 1B—lower chamber, 1C—screw,
11—进气口,12—出气口;11—air inlet, 12—air outlet;
20—差分气路单元,20—differential air circuit unit,
21—第1三通电磁阀,22—第2三通电磁阀,23—测量室,24—气泵;21—the first three-way solenoid valve, 22—the second three-way solenoid valve, 23—measurement chamber, 24—air pump;
30—电源;30—Power supply;
40—传感器阵列,40—sensor array,
41—CO2传感器,42—温湿度传感器,43—气体流速传感器,41—CO 2 sensor, 42—temperature and humidity sensor, 43—gas flow rate sensor,
44—气体压强传感器;44—gas pressure sensor;
50—微控制平台,50—Micro control platform,
51—ADC采样模块,52—泵速调控板,53—无线发射模块。51—ADC sampling module, 52—pump speed control board, 53—wireless transmitting module.
具体实施方式Detailed ways
下面结合附图和实施例对本发明作进一步说明。The present invention will be further described below with reference to the accompanying drawings and embodiments.
一、检测仪1. Detector
1、总体1. Overall
如图1,本发明包括被测对象——叶片00,设置有叶室10、差分气路单元20、电源30、传感器阵列40和微控制平台50;As shown in FIG. 1 , the present invention includes an object to be measured, a
被测叶片00置于叶室10内,叶室10和差分气路单元20连通,在差分气路单元20的测量室23内设置有传感器阵列40,传感器阵列40和微控制平台50连接;The
电源30给传感器阵列40和微控制平台50提供能源。
2、功能部件2. Functional components
0)叶片000)
1)叶室101)
如图2,叶室10包括依次连接的上叶室1A、下叶室1B和螺杆1C,上叶室1A设有进气口11和出气口12,在上叶室1A的玻璃片上画有均匀网格,用来测量被测植株叶片过小而无法填满整个叶室时的叶片面积,每一小格面积为1mm2,网格总面积为900mm2;下叶室1B与螺杆1C连接,通过旋转螺杆1C来控制下叶室1A上下移动,在上、下叶室1A、1B的接触部分贴有橡皮垫用来优化叶室10气密性和保护被测植株叶片00被夹时不会破损。As shown in FIG. 2, the
叶室10还设置有控制手柄,通过控制手柄控制叶室10的开合。The
2)差分气路单元202) Differential
如图1,差分气路单元20包括第1三通电磁阀21、第2三通电磁阀22、测量室23和气泵24;As shown in FIG. 1 , the differential
互为连通的第1三通电磁阀21和第2三通电磁阀22分别连接叶室10的进气口11和出气口12,第2三通电磁阀22、测量室23和气泵24依次连通;The first three-
在测量室23中设置传感器阵列40。A
3)电源303)
如图1,电源30为大容量锂离子可充电池,输入电压为12.6VDC,输出电压为12.6~10.8VDC,容量6800mAh。As shown in FIG. 1 , the
4)传感器阵列404)
如图1,传感器阵列40包括CO2传感器41、温湿度传感器42、气体流速传感器43和气体压强传感器44。As shown in FIG. 1 , the
(1)CO2传感器41(1) CO 2 sensor 41
为通用的传感器,测量CO2的浓度。A general purpose sensor that measures the concentration of CO2 .
(2)温湿度传感器42(2) Temperature and humidity sensor 42
为通用的传感器,测量温湿度。It is a general-purpose sensor that measures temperature and humidity.
(3)气体流速传感器43(3) Gas flow rate sensor 43
为通用的传感器,测量气体流速。A general purpose sensor that measures gas flow rates.
(4)气体压强传感器44(4) Gas pressure sensor 44
为通用的传感器,测量气体压强。It is a general-purpose sensor that measures gas pressure.
5)微控制平台505)
如图1,微控制平台50选用MSP430F149作为CPU,来处理数据和控制其他模块;内嵌有ADC采样模块51、泵速调控板52和无线发射模块 53。As shown in Figure 1, the
接收经过ADC采样模块51处理的数字信号,控制泵速调控板52使气泵24工作,将数据传送至无线发射模块53。Receive the digital signal processed by the
3、工作机理3. Working mechanism
通过传感器阵列中的CO2传感器、气体流速传感器、大气压强传感器和温湿度传感器与ADC采样模块相连,将检测叶室内的CO2浓度、空气流速、大气压强和温湿度转化为电信号,然后通过AD模数转换,将电信号转化为微控制平台可识别的数字信号,微控制平台对数字信号进行分析处理,进而控制气泵工作,并用蓝牙无线发射模块将数据发送到智能移动设备上生成植物光合速率曲线图。The CO2 sensor, gas flow rate sensor, atmospheric pressure sensor and temperature and humidity sensor in the sensor array are connected to the ADC sampling module, and the CO2 concentration, air flow rate, atmospheric pressure and temperature and humidity in the detected leaf chamber are converted into electrical signals, and then passed through the AD Analog-to-digital conversion converts electrical signals into digital signals that can be recognized by the micro-control platform. The micro-control platform analyzes and processes the digital signals, and then controls the air pump to work, and uses the Bluetooth wireless transmitter module to send the data to the smart mobile device to generate the photosynthetic rate of plants. Graph.
二、检测方法2. Detection method
①对检测系统初始化:①Initialize the detection system:
Ⅰ、开启电源30,让各模块正常工作;Ⅰ. Turn on the
Ⅱ、设置第1三通电磁阀21的端口a、b和第2三通电磁阀22的端口e、d导通,使气流在气路中按照虚线箭头方向流动,即使气流按第1三通电磁阀21、第2三通电磁阀22、测量室23和气泵24依次连接的方向流动;II. Set the ports a and b of the first three-
Ⅲ、使传感器阵列40正常工作,并将各传感器的检测数据传入微控制平台40,微控制平台40根据大气压强传感器43和气体流速传感器42检测的数据,来调控气泵24工作,控制差分气路单元20内气体流速;III. Make the
Ⅳ、CO2传感器41测得数据经过卡尔曼算法滤波处理和ADC采样模块51数模转换,通过蓝牙无线发射模块53上传至智能移动设备上,若测得CO2浓度随时间基本不变,即系统初始化完成;IV. The data measured by the CO 2 sensor 41 is filtered and processed by the Kalman algorithm and digital-to-analog conversion by the
②系统初始化完成之后,开始检测被测植株的光合速率,包括以下几个步骤:②After the system initialization is completed, start to detect the photosynthetic rate of the tested plant, including the following steps:
ⅰ、按下叶室10开合控制手柄按钮,夹紧叶片00,若叶片00接触面积太小无法填满整个叶室10,则数出叶室10中叶片所占的上叶室1A玻璃片上的小网格格数,每一个小网格面积为1mm2,估算出叶片00的面积,若叶片00可以填满整个叶室10,则叶片00面积可视为整个叶室面积1600mm2;ⅰ. Press the opening and closing control handle button of the
ⅱ、设置第1三通电磁阀21的端口a、c导通,端口b关闭,第2三通电磁阀22的端口d、f导通,端口e关闭,使气流在气路中按照实线线箭头方向流动,即使气流按第1三通电磁阀21、叶室10、第2三通电磁阀22、测量室23和气泵24依次连接的方向流动;ii. Set the ports a and c of the first three-
ⅲ、保持测量一段时间,打开智能移动设备的蓝牙功能,接收检测仪通过蓝牙发送的数据,收集一段时间的数据即可生成植物光合速率曲线图。3. Keep measuring for a period of time, turn on the Bluetooth function of the smart mobile device, receive the data sent by the detector through Bluetooth, and collect the data for a period of time to generate a plant photosynthetic rate curve.
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CN113418888A (en) * | 2021-06-21 | 2021-09-21 | 安徽农业大学 | Modularized plant photosynthesis detector |
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CN114814104A (en) * | 2022-04-28 | 2022-07-29 | 湖南杂交水稻研究中心 | Device for inducing photosynthesis of single-sided leaves |
CN115307951A (en) * | 2021-05-06 | 2022-11-08 | 中国科学院分子植物科学卓越创新中心 | Plant leaf original state rapid freezing sampling device, sampling system and sampling method |
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