CN111965309A - Portable plant photosynthetic rate detector and method thereof - Google Patents

Abstract

The invention discloses a portable plant photosynthetic rate detector and a method thereof, and relates to the field of plant physiological function detection. The detector includes a blade (00), which is provided with a blade chamber (10), a differential air circuit unit (20), a power supply (30), a sensor array (40) and a micro-control platform (50); the blade (00) is placed on the blade Inside the chamber (10), the leaf chamber (10) is communicated with the differential air circuit unit (20), and a sensor array (40) is arranged in the measurement chamber (23) of the differential air circuit unit (20), the sensor array (40) and The micro-control platform (50) is connected; the power supply (30) provides energy to the sensor array (40) and the micro-control platform (50). Compared with the prior art, the present invention has the following advantages and positive effects: 1. the photosynthetic rate of plants can be measured quickly, accurately and in real time under different environmental conditions of CO ₂ concentration, light intensity and temperature and humidity; 2. lightweight structure, It is easy to carry and has a wide range of applications; ③It supports outdoor use.

Description

Portable plant photosynthetic rate detector and method thereof

technical field

The invention relates to the field of plant physiological function detection, in particular to a portable plant photosynthetic rate detector and a method thereof.

Background technique

Photosynthesis is an important feature of the growth and development of green plants. Measuring the photosynthetic rate of plants is an important indicator for judging the physiological characteristics of plants. The photosynthetic rate of plants can be used as a data for judging the suitable living environment of plants.

When plants perform photosynthesis, they absorb _CO2 and water from the environment, produce carbohydrates and release oxygen. When plants perform photosynthesis, the light intensity and CO ₂ concentration in the environment are the main factors that affect the photosynthetic rate of plants, and the temperature and humidity of the environment will also affect the photosynthetic rate of plants.

The traditional methods of measuring the photosynthetic rate of plants are:

1. Improved half-leaf method. This method must separate the leaves from the plants, and then measure them. The operation is complicated, the experimental error is large, and the photosynthetic rate of plants cannot be measured in real time.

2. The gas volume change method, this method can only measure some small plants, and the scope of application is limited.

3. Infrared CO ₂ analysis method, this method is affected by the measurement site and cannot measure the photosynthetic rate of outdoor plants.

SUMMARY OF THE INVENTION

The purpose of the present invention is to overcome the shortcomings and deficiencies existing in the prior art, and to provide a portable plant photosynthetic rate detector and a method thereof, which can quickly, accurately and real-time measure the changes of plants in different _CO2 concentrations, light intensity and temperature and humidity. Photosynthetic rate under ambient conditions.

The object of the present invention is achieved in this way:

The detector includes a blade, which is provided with a blade chamber, a differential air circuit unit, a power supply, a sensor array and a micro-control platform;

The blade to be tested is placed in the blade chamber, the blade chamber is connected with the differential air circuit unit, a sensor array is arranged in the measurement chamber of the differential air circuit unit, and the sensor array is connected with the micro-control platform;

The power supply provides energy to the sensor array and the microcontroller platform.

Compared with the prior art, the present invention has the following advantages and positive effects:

①The photosynthetic rate of plants can be measured quickly, accurately and in real time under different environmental conditions of CO ₂ concentration, light intensity and temperature and humidity;

②The structure is light, easy to carry, and has a wide range of applications;

③Support outdoor use.

Description of drawings

Fig. 1 is the structural representation of this detector;

Figure 2 is a schematic diagram of the structure of the leaf chamber.

In the picture:

00—leaf;

10—leaf chamber,

1A—upper chamber, 1B—lower chamber, 1C—screw,

11—air inlet, 12—air outlet;

20—differential air circuit unit,

21—the first three-way solenoid valve, 22—the second three-way solenoid valve, 23—measurement chamber, 24—air pump;

30—Power supply;

40—sensor array,

41—CO ₂ sensor, 42—temperature and humidity sensor, 43—gas flow rate sensor,

44—gas pressure sensor;

50—Micro control platform,

51—ADC sampling module, 52—pump speed control board, 53—wireless transmitting module.

Detailed ways

The present invention will be further described below with reference to the accompanying drawings and embodiments.

1. Detector

1. Overall

As shown in FIG. 1 , the present invention includes an object to be measured, a blade 00, which is provided with a blade chamber 10, a differential air circuit unit 20, a power supply 30, a sensor array 40 and a micro-control platform 50;

The blade 00 to be tested is placed in the blade chamber 10, the blade chamber 10 is communicated with the differential air circuit unit 20, a sensor array 40 is arranged in the measurement chamber 23 of the differential air circuit unit 20, and the sensor array 40 is connected with the micro-control platform 50;

Power supply 30 provides power to sensor array 40 and microcontroller platform 50 .

2. Functional components

0) Blade 00

1) Leaf chamber 10

As shown in FIG. 2, the leaf chamber 10 includes an upper leaf chamber 1A, a lower leaf chamber 1B and a screw 1C which are connected in sequence. The upper leaf chamber 1A is provided with an air inlet 11 and an air outlet 12, and the glass sheet of the upper leaf chamber 1A is drawn with uniform The grid is used to measure the leaf area of the tested plant when the leaves are too small to fill the entire leaf chamber. The area of each small grid is 1 mm ² , and the total grid area is 900 mm ² ; The lower leaf chamber 1A is controlled to move up and down by rotating the screw 1C, and rubber pads are attached to the contact parts of the upper and lower leaf chambers 1A and 1B to optimize the air tightness of the leaf chamber 10 and protect the tested plant leaves 00 from being clamped. damaged.

The leaf chamber 10 is also provided with a control handle, and the opening and closing of the leaf chamber 10 is controlled by the control handle.

2) Differential air circuit unit 20

As shown in FIG. 1 , the differential air circuit unit 20 includes a first three-way solenoid valve 21 , a second three-way solenoid valve 22 , a measurement chamber 23 and an air pump 24 ;

The first three-way solenoid valve 21 and the second three-way solenoid valve 22 which are in communication with each other are respectively connected to the air inlet 11 and the air outlet 12 of the leaf chamber 10, and the second three-way solenoid valve 22, the measuring chamber 23 and the air pump 24 are connected in sequence. ;

A sensor array 40 is arranged in the measurement chamber 23 .

3) Power 30

As shown in FIG. 1 , the power source 30 is a large-capacity lithium-ion rechargeable battery, the input voltage is 12.6VDC, the output voltage is 12.6-10.8VDC, and the capacity is 6800mAh.

4) Sensor array 40

As shown in FIG. 1 , the sensor array 40 includes a CO ₂ sensor 41 , a temperature and humidity sensor 42 , a gas flow rate sensor 43 and a gas pressure sensor 44 .

(1) CO ₂ sensor 41

A general purpose sensor that measures the concentration of _CO2 .

(2) Temperature and humidity sensor 42

It is a general-purpose sensor that measures temperature and humidity.

(3) Gas flow rate sensor 43

A general purpose sensor that measures gas flow rates.

(4) Gas pressure sensor 44

It is a general-purpose sensor that measures gas pressure.

5) Micro Control Platform 50

As shown in Figure 1, the micro control platform 50 selects MSP430F149 as the CPU to process data and control other modules;

Receive the digital signal processed by the ADC sampling module 51 , control the pump speed control board 52 to make the air pump 24 work, and transmit the data to the wireless transmission module 53 .

3. Working mechanism

The CO2 sensor, gas flow rate sensor, atmospheric pressure sensor and temperature and humidity sensor in the sensor array are connected to the ADC sampling module, and the _CO2 concentration, air flow rate, atmospheric pressure and temperature and humidity in the detected leaf chamber are converted into electrical signals, and then passed through the AD Analog-to-digital conversion converts electrical signals into digital signals that can be recognized by the micro-control platform. The micro-control platform analyzes and processes the digital signals, and then controls the air pump to work, and uses the Bluetooth wireless transmitter module to send the data to the smart mobile device to generate the photosynthetic rate of plants. Graph.

2. Detection method

①Initialize the detection system:

Ⅰ. Turn on the power supply 30 to make each module work normally;

II. Set the ports a and b of the first three-way solenoid valve 21 and the ports e and d of the second three-way solenoid valve 22 to conduct, so that the air flows in the direction of the dashed arrow in the air path, even if the air flows in the direction of the first three-way solenoid valve The solenoid valve 21, the second three-way solenoid valve 22, the measuring chamber 23 and the air pump 24 flow in the direction in which they are connected in sequence;

III. Make the sensor array 40 work normally, and transmit the detection data of each sensor to the micro-control platform 40. The micro-control platform 40 regulates the work of the air pump 24 according to the data detected by the atmospheric pressure sensor 43 and the gas flow rate sensor 42, and controls the differential gas the gas flow rate in the circuit unit 20;

IV. The data measured by the CO ₂ sensor 41 is filtered and processed by the Kalman algorithm and digital-to-analog conversion by the ADC sampling module 51, and uploaded to the smart mobile device through the Bluetooth wireless transmission module 53. If the measured CO ₂ concentration is basically unchanged with time, that is, System initialization is complete;

②After the system initialization is completed, start to detect the photosynthetic rate of the tested plant, including the following steps:

ⅰ. Press the opening and closing control handle button of the leaf chamber 10 to clamp the leaf 00. If the contact area of the leaf 00 is too small to fill the entire leaf chamber 10, count the upper chamber 1A glass sheet occupied by the leaves in the leaf chamber 10. The number of small grids, each small grid area is 1mm ² , the area of the blade 00 is estimated, if the blade 00 can fill the entire leaf chamber 10, the area of the blade 00 can be regarded as the entire leaf chamber area 1600mm ² ;

ii. Set the ports a and c of the first three-way solenoid valve 21 to be turned on, the port b to be closed, the ports d and f of the second three-way solenoid valve 22 to be turned on, and the port e to be closed, so that the air flow in the gas path follows the solid line Flow in the direction of the line arrow, even if the airflow flows in the direction in which the first three-way solenoid valve 21, the leaf chamber 10, the second three-way solenoid valve 22, the measuring chamber 23 and the air pump 24 are connected in sequence;

3. Keep measuring for a period of time, turn on the Bluetooth function of the smart mobile device, receive the data sent by the detector through Bluetooth, and collect the data for a period of time to generate a plant photosynthetic rate curve.

Claims (7)

1. A portable plant photosynthetic rate detector, comprising a blade (00), characterized in that: A leaf chamber (10), a differential air circuit unit (20), a power supply (30), a sensor array (40) and a micro-control platform (50) are provided; The blade (00) is placed in the blade chamber (10), the blade chamber (10) is communicated with the differential air circuit unit (20), and a sensor array (40) is arranged in the measurement chamber (23) of the differential air circuit unit (20) , the sensor array (40) is connected with the micro-control platform (50); A power supply (30) provides energy to the sensor array (40) and the microcontroller platform (50). 2. by the portable plant photosynthetic rate detector according to claim 1, it is characterized in that: The vane chamber (10) includes an upper vane chamber (1A), a lower vane chamber (1B) and a screw (1C), and the upper vane chamber (1A) is provided with an air inlet (11) and an air outlet (12). A uniform grid is drawn on the glass sheet of the upper leaf chamber (1A), which is used to measure the leaf area of the tested plant when the leaves are too small to fill the entire leaf chamber. The area of each small grid is 1 mm ² , and the total grid area is 900 mm. ² ; The lower chamber (1B) is connected with the screw (1C), and the lower chamber (1A) is controlled to move up and down by rotating the screw (1C), and a rubber pad is attached to the contact part of the upper and lower (1A, 1B) chambers To optimize the air tightness of the leaf chamber and protect the tested plant leaves (00) from being damaged when they are clamped; The leaf chamber (10) is also provided with a control handle, through which the opening and closing of the leaf chamber 10 is controlled. 3. by the described portable plant photosynthetic rate detector of claim 1, it is characterized in that: The differential air circuit unit (20) includes a first three-way solenoid valve (21), a second three-way solenoid valve (22), a measuring chamber (23) and an air pump (24); The first three-way solenoid valve (21) and the second three-way solenoid valve (22) that communicate with each other are respectively connected to the air inlet (11) and the air outlet (12) of the leaf chamber (10), and the second three-way solenoid valve (22), the measuring chamber (23) and the air pump (24) are communicated in sequence; A sensor array (40) is arranged in the measurement chamber (23). 4. by the described portable plant photosynthetic rate detector of claim 1, it is characterized in that: The power supply (30) is a large-capacity lithium-ion rechargeable battery with an input voltage of 12.6VDC, an output voltage of 12.6-10.8VDC, and a capacity of 6800mAh. 5. by the portable plant photosynthetic rate detector according to claim 1, it is characterized in that: The sensor array (40) includes a CO ₂ sensor (41), a temperature and humidity sensor (42), a gas flow rate sensor (43) and a gas pressure sensor (44). 6. by the described portable plant photosynthetic rate detector of claim 1, it is characterized in that: The micro-control platform (50) selects MSP430F149 as a CPU to process data and control other modules; an ADC sampling module (51), a pump speed control board (52) and a wireless transmission module (53) are embedded in it Receive the digital signal processed by the ADC sampling module (51), control the pump speed control board (52) to make the air pump (24) work, and transmit the data to the wireless transmission module (53). 7. based on the detection method of the described portable plant photosynthetic rate detector of claim 1,2,3,4,5 or 6, it is characterized in that: ①Initialize the detection system: Ⅰ. Turn on the power and let each module work normally; Ⅱ. Set the ports a and b of the first three-way solenoid valve (21) and the ports e and d of the second three-way solenoid valve (22) to conduct, so that the air flow is in accordance with the first three-way solenoid valve (21) and the second three-way solenoid valve (21). The three-way solenoid valve (22), the measuring chamber (23) and the air pump (24) flow in the direction in which they are connected in sequence; III. The sensor array (40) works normally and transmits the detection data of each sensor to the micro-control platform (50), and the micro-control platform (50) regulates and controls according to the data detected by the gas flow rate sensor (42) and the atmospheric pressure sensor (43). The air pump (24) works to control the gas flow rate in the differential air circuit unit (20); IV. The data measured by the _{CO 2} sensor (41) is filtered and processed by the Kalman algorithm and digital-to-analog conversion by the ADC sampling module (51), and uploaded to the smart mobile device through the Bluetooth wireless transmission module (53). The time is basically unchanged, that is, the system initialization is completed; ②After the system initialization is completed, start to detect the photosynthetic rate of the tested plant, including the following steps: ⅰ. Press the opening and closing control handle button of the leaf chamber (10) to clamp the blade (00). If the contact area of the blade (00) is too small to fill the entire leaf chamber (10), count out the The number of small grids on the glass sheet of the upper chamber occupied by the blade, and the area of each small grid is 1mm ² . Calculate the area of the blade. If the blade can fill the entire chamber (10), the area of the blade (00) can be It is regarded as the entire leaf chamber area of 1600mm2; ii. Set the ports a and c of the first three-way solenoid valve (21) to be turned on, the port b to be closed, the ports d and f of the second three-way solenoid valve (22) to be turned on, and the port e to be closed, so that the air flows in the air path. flow in the direction in which the first three-way solenoid valve (21), the leaf chamber (10), the second three-way solenoid valve (22), the measuring chamber (23) and the air pump (24) are connected in sequence; iii. Keep measuring for a period of time, turn on the bluetooth function of the smart mobile device, receive the data sent by the detector through bluetooth, and generate a graph of plant photosynthetic rate.

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