CN111965288A - Method for determining ethanol content by gas chromatography in non-aqueous medium - Google Patents
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 205
- 238000000034 method Methods 0.000 title claims abstract description 32
- 238000004817 gas chromatography Methods 0.000 title claims abstract description 23
- 239000012736 aqueous medium Substances 0.000 title claims abstract description 9
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 claims abstract description 57
- 238000001514 detection method Methods 0.000 claims abstract description 51
- ZXEKIIBDNHEJCQ-UHFFFAOYSA-N isobutanol Chemical compound CC(C)CO ZXEKIIBDNHEJCQ-UHFFFAOYSA-N 0.000 claims abstract description 50
- 239000000126 substance Substances 0.000 claims abstract description 36
- 239000002904 solvent Substances 0.000 claims abstract description 32
- 238000002347 injection Methods 0.000 claims abstract description 8
- 239000007924 injection Substances 0.000 claims abstract description 8
- 239000000523 sample Substances 0.000 claims description 44
- 239000012086 standard solution Substances 0.000 claims description 31
- 239000012488 sample solution Substances 0.000 claims description 15
- 238000012937 correction Methods 0.000 claims description 10
- 239000007789 gas Substances 0.000 claims description 7
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 5
- 239000002202 Polyethylene glycol Substances 0.000 claims description 5
- 229920001223 polyethylene glycol Polymers 0.000 claims description 5
- 230000005526 G1 to G0 transition Effects 0.000 claims description 4
- 235000019441 ethanol Nutrition 0.000 description 61
- 239000000243 solution Substances 0.000 description 12
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 11
- 238000000926 separation method Methods 0.000 description 7
- 238000011084 recovery Methods 0.000 description 6
- 230000007774 longterm Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 239000000203 mixture Substances 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 238000004587 chromatography analysis Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000011895 specific detection Methods 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 208000033962 Fontaine progeroid syndrome Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000010813 internal standard method Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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- G—PHYSICS
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- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
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Abstract
The invention discloses a method for determining ethanol content by gas chromatography in a non-aqueous medium, which adopts isobutanol as an internal standard substance and N, N-dimethylformamide as a solvent, and has the following gas chromatography detection conditions: an InertCap WAX capillary column with the specification of 30m multiplied by 0.25mm and 0.25 mu m; column temperature: the initial temperature is 100 ℃, the temperature is maintained for 5min, the temperature is increased to 240 ℃ at the speed of 120 ℃/min, and the temperature is maintained for 5 min; the split ratio is as follows: 10: 1; sample inlet temperature: 250 ℃; detector temperature: 250 ℃, injection volume 0.5-1 μ L, column flow rate: 1.41mL/min, etc., and the method can reduce the loss of effective components of the chromatographic column, improve the detection stability and prolong the service life of the chromatographic column on the basis of ensuring the detection accuracy.
Description
Technical Field
The invention relates to ethanol content detection, in particular to a method for determining ethanol content by gas chromatography in a non-aqueous medium.
Background
The alcohol type no-clean hand sanitizer is convenient to take and clean, is gradually a substitute of perfumed soap, and is popular with people. Alcohol in the alcohol type no-clean hand sanitizer is a main sterilization and disinfection component, and aims to effectively detect the content of the alcohol in the hand sanitizer and ensure the disinfection effect. At present, the most accurate method for detecting ethanol is gas chromatography, water is generally used as a solvent, and in order to improve the detection accuracy in ethanol detection, particularly in the detection of the content of ethanol in gel hand sanitizer, n-propanol is used as an internal standard substance in the prior art. However, the conventional gas chromatography detection method is not good in long-term accuracy.
Therefore, the method for measuring the ethanol content by the gas chromatography with high long-term and short-term accuracy has great significance for detecting the ethanol.
Disclosure of Invention
In the existing gas chromatography, water is used as a solvent, and n-propanol or an internal standard substance is not used, so that the content of ethanol in the gel hand sanitizer is detected. The inventor of the invention researches and discovers that although the conventional method has better accuracy in the initial detection stage, the detection result in the later detection stage has larger deviation along with the prolonging of the detection time. In view of the above, the inventors of the present invention have found through research that, the existing gas chromatography ethanol methods all use water as a solvent, and because the existing gas chromatography columns such as DB-WAX and HP-WAX generally use polyethylene glycol packing as a stationary phase, the loss of polyethylene glycol is easily caused in an aqueous medium, which reduces the long-term accuracy of gas chromatography detection and also reduces the lifetime of the chromatography column. Therefore, it is very necessary to provide a method with less influence on the packing of the gas chromatography column, on the basis of ensuring the detection accuracy, to protect the loss of the effective components of the chromatography column, and to improve the detection stability and the service life of the chromatography column.
The invention aims to provide a method for measuring the content of ethanol by gas chromatography in a non-aqueous medium, which can reduce the loss of effective components of a chromatographic column, improve the detection stability and prolong the service life of the chromatographic column on the basis of ensuring the detection accuracy.
In order to achieve the aim, the invention provides a method for measuring the ethanol content by gas chromatography in a non-aqueous medium, which adopts isobutanol as an internal standard substance and N, N-dimethylformamide as a solvent, and has the following gas chromatography detection conditions: an InertCap WAX capillary column with the specification of 30m multiplied by 0.25mm and 0.25 mu m; column temperature: the initial temperature is 100 ℃, the temperature is maintained for 5min, the temperature is increased to 240 ℃ at the speed of 120 ℃/min, and the temperature is maintained for 5 min; the split ratio is as follows: 10: 1; sample inlet temperature: 250 ℃; detector temperature: 250 ℃, injection volume 0.5-1 μ L, column flow rate: 1.41mL/min, line speed: 35 cm/sec; hydrogen gas: 30 mL/min; air: 400mL/min, tail-blown: 12 mL/min.
According to the technical scheme, the isobutanol is used as an internal standard substance, the N, N-dimethylformamide is used as a solvent, the ethanol content in the sample is detected under the specific detection conditions, the method is high in detection accuracy, and the detection stability and the service life of the chromatographic column can be improved on the basis of ensuring the detection accuracy. The reason for this is that the flow using N, N-dimethylformamide as a solvent has a smaller influence than that of an InertCap WAX capillary column, and the separation degree of N, N-dimethylformamide to a sample peak is higher, so that the method has high detection accuracy, and can improve the detection stability and the service life of a chromatographic column on the basis of ensuring the detection accuracy.
Additional features and advantages of the invention will be set forth in the detailed description which follows.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a chromatogram of an ethanol solution using isobutanol as an internal standard, with DMF as the solvent.
Detailed Description
The following describes in detail specific embodiments of the present invention. It should be understood that the detailed description and specific examples, while indicating the present invention, are given by way of illustration and explanation only, not limitation.
The endpoints of the ranges and any values disclosed herein are not limited to the precise range or value, and such ranges or values should be understood to encompass values close to those ranges or values. For ranges of values, between the endpoints of each of the ranges and the individual points, and between the individual points may be combined with each other to give one or more new ranges of values, and these ranges of values should be considered as specifically disclosed herein.
The invention provides a method for determining ethanol content by gas chromatography in a non-aqueous medium, which adopts isobutanol as an internal standard substance and N, N-dimethylformamide as a solvent, and has the following gas chromatography detection conditions: an InertCap WAX capillary column with the specification of 30m multiplied by 0.25mm and 0.25 mu m; column temperature: the initial temperature is 100 ℃, the temperature is maintained for 5min, the temperature is increased to 240 ℃ at the speed of 120 ℃/min, and the temperature is maintained for 5 min; the split ratio is as follows: 10: 1; sample inlet temperature: 250 ℃; detector temperature: 250 ℃, injection volume 0.5-1 μ L, column flow rate: 1.41mL/min, line speed: 35 cm/sec; hydrogen gas: 30 mL/min; air: 400mL/min, tail-blown: 12 mL/min.
According to the technical scheme, the isobutanol is used as an internal standard substance, the N, N-dimethylformamide is used as a solvent, the ethanol content in the sample is detected under the specific detection conditions, the method is high in detection accuracy, and the detection stability and the service life of the chromatographic column can be improved on the basis of ensuring the detection accuracy. The reason for this is that the flow using N, N-dimethylformamide as a solvent has a smaller influence than that of an InertCap WAX capillary column, and the separation degree of N, N-dimethylformamide to a sample peak is higher, so that the method has high detection accuracy, and can improve the detection stability and the service life of a chromatographic column on the basis of ensuring the detection accuracy.
According to the method, the method has high detection accuracy, the specific formula for detection can adopt the conventional supply in the field, and the content of the ethanol in the sample can be calculated as long as the peak area detected in the chromatogram can be matched with the content of the sample. In a preferred embodiment of the present invention, in order to improve the accuracy of detection, it is preferable that the present invention comprises the following steps: (1) preparing a standard solution by using ethanol and isobutanol as an internal standard substance as solutes and using N, N-dimethylformamide as a solvent; (2) preparing a sample solution containing an internal standard substance; (3) measuring peak areas of an internal standard substance and ethanol in the standard solution and the sample solution respectively by using a gas chromatograph, and calculating a mass correction factor f of the ethanol in the standard solution relative to the internal standard substance according to the following formulan：
fn＝Wn*AS/Ws*An
In the formula WsThe mass of the internal standard substance in the standard solution;
Wnthe mass of ethanol in the standard solution;
ASis the peak area of the internal standard in the standard solution;
Anis the peak area of ethanol in the standard solution;
(4) calculating the content of ethanol in the sample C%:
C％＝Ws*Ai*fn*100％/Wsp*Ais
wherein Wis is the mass of an internal standard substance in the sample solution;
Wspis the total mass of the sample;
Aiis the peak area of ethanol in the sample;
Aisfor the sample solutionPeak area of the subject;
fnis the mass correction factor for ethanol in the sample.
The mass ratio of the ethanol in the standard solution to the added internal standard substance can be selected in a wider range, and in order to improve the detection accuracy, the mass ratio of the ethanol in the standard solution to the added internal standard substance is preferably 0.3-3: 1; further preferably, the mass ratio of ethanol to the added internal standard in the standard solution is 0.5-2: 1.
The mass ratio of the total weight of the sample to the added internal standard substance can be selected in a wide range, and in order to improve the detection accuracy, the mass ratio of the total weight of the sample to the added internal standard substance is preferably 0.3-3: 1; preferably, the mass ratio of the total weight of the sample to the added internal standard is 0.5-2: 1.
The sample of the present invention may be a solution containing ethanol and/or a gel containing ethanol, such as a gel-type hand sanitizer, and preferably, the content of ethanol in the sample is in the range of 40 wt% or more. The detection method is suitable for detecting the ethanol in the conventional gel-type ethanol disinfectant.
In order to further improve the accuracy of detection, it is preferable that the concentration of ethanol in the standard solution is 0.16 to 0.24 g/mL.
The gas chromatographic column is suitable for chromatographic columns of various models of InertCap WAX, and preferably, the gas chromatographic column not only shows higher accuracy, but also has higher detection stability when the stationary phase of the capillary column is polyethylene glycol.
Further preferably, the InertCap WAX capillary column employed in the present invention is DB-WAX or HP-WAX.
In the above technical solution, the selection of the sample amount can be adjusted in a wide range, and preferably, the sample amount is 0.5-0.8 μ L.
The present invention will be described in detail below by way of examples. In the examples, the Shimadzu GCMS-QP2010SE gas chromatograph-mass spectrometer was used; AOC-20i type automatic sample injector; the hydrogen flame ionization detector, i.e., isobutanol, N-Dimethylformamide (DMF), N-propanol and absolute ethanol, were all commercially available analytical grade.
Example 1
The chromatographic conditions of the gas chromatography internal standard method are as follows:
a chromatographic column: InertCap WAX capillary column (30 m.times.0.25 mm, 0.25 μm), column temperature: the initial temperature is 100 ℃, the temperature is maintained for 5min, the temperature is increased to 240 ℃ at the speed of 120 ℃/min, the temperature is maintained for 5min, and the split ratio: 10:1, injection port temperature: 250 ℃, detector temperature: 250 ℃, injection volume 0.5 μ L, column flow rate: 1.41mL/min, line speed: 35cm/sec, hydrogen: 30mL/min, air: 400mL/min, tail-blown: 12 mL/min.
1. Preparation of standard solution and calculation of quality correction factor:
5 solutions with different concentrations are prepared according to the formula in the table 1 as standard solutions, and the specific preparation process is as follows: 60g of absolute ethyl alcohol is weighed into a 100mL volumetric flask, DMF is used as a solvent, and the mixture is shaken up in constant volume. 60g of isobutanol was weighed into another 100-volume flask and shaken to a constant volume using DMF as solvent. Transferring the solutions into volumetric flasks of 10.0mL to 25mL respectively by using a pipette, fixing the volume to the scale by using DMF as a solvent, shaking up, and standing. Injecting 0.5 μ L of standard solution, measuring peak areas of internal standard substance and ethanol of the standard solution with gas chromatograph under the above conditions, and calculating mass correction factor f of each component in the standard solution relative to the internal standard substance according to the following formulan：
fn＝Wn*AS/Ws*An
In the formula WsThe mass of the internal standard substance in the standard solution;
Wnthe mass of ethanol in the standard solution;
ASis the peak area of the internal standard in the standard solution;
Anis the peak area of ethanol in the standard solution.
TABLE 1
| Serial number | Isobutanol/g | Ethanol/g |
| 1 | 60.0109 | 66.7835 |
| 2 | 60.0109 | 57.8115 |
| 3 | 60.0109 | 54.8170 |
| 4 | 60.0109 | 56.4170 |
| 5 | 60.0109 | 62.1513 |
The ethanol relative mass correction factor was calculated according to the above formula and the results are shown in Table 2.
TABLE 2
As can be seen from table 2, the RSD relative to the mass correction factor was 0.77% (n ═ 5), indicating good process precision.
2. Determination of the ethanol content in the sample:
a commercial wash-free hand sanitizer containing 55-70% of alcohol is selected for actual sample test, 40g of the wash-free hand sanitizer containing 55-70% of alcohol is weighed into a 100mL volumetric flask, and DMF is used as a solvent for constant volume and shaking up. 60g of isobutanol is weighed into a 100mL volumetric flask, the volume is determined by using DMF as a solvent, and the mixture is shaken up. Transferring the solutions into volumetric flasks of 10.0mL to 25mL respectively by using a pipette, carrying out constant volume by using DMF as a solvent, shaking up, and preparing into sample solutions.
Peak areas of the internal standard substance and ethanol in the above sample solution were measured by gas chromatography, and the content C% of ethanol in the sample was calculated according to the following formula, and the results are shown in table 3:
C％＝Ws*Ai*fn*100％/Wsp*Ais
wherein Wis is the mass of an internal standard substance in the sample solution;
Wspis the total mass of the sample;
Aiis the peak area of ethanol in the sample;
Aisis the peak area of the internal standard substance in the sample solution;
fnis the average of the mass correction factors for ethanol in the sample, i.e., the relative mass correction factors in table 3.
TABLE 3
Through tests, the absolute difference value of the measurement results of the two parallel samples is 0.1%, the parallelism result is satisfactory, and the repeatability of the method is better.
Detection example 1
Determination of precision:
preparing a sample solution with known content, weighing 60g of absolute ethyl alcohol into a 100mL volumetric flask, using DMF as a solvent to fix the volume, and shaking up. 60g of isobutanol is weighed into a 100mL volumetric flask, the volume is determined by using DMF as a solvent, and the mixture is shaken up. Transferring the solutions into volumetric flasks of 10.0mL to 25mL respectively by using a pipette, carrying out constant volume by using DMF as a solvent, shaking up, and preparing into sample solutions. The samples were separately injected by gas chromatography and measured 2 times as described above, and the measurement results are shown in Table 4.
TABLE 4
Therefore, the method has good reproducibility and repeatability and can meet the daily analysis requirement.
Detection example 2
And (3) standard addition recovery test:
in order to verify the detection accuracy by using isobutanol as an internal standard and DMF as a solvent and applying the method disclosed by the invention, 40g of absolute ethyl alcohol is weighed into a 100mL volumetric flask, and DMF is used as the solvent to fix the volume and shake up. 60g of isobutanol is weighed into a 100mL volumetric flask, the volume is determined by using DMF as a solvent, and the mixture is shaken up. Transferring the solutions into volumetric flasks of 10.0mL to 25mL respectively by using a pipette, carrying out constant volume by using DMF as a solvent, shaking up, and preparing into sample solutions. The content of ethanol in the known solution was measured by the above-mentioned gas chromatograph, and the results of the recovery of the analyzed samples are shown in Table 5.
TABLE 5
| Serial number | Sample ethanol content (g) | Determination of the ethanol content (g) | Recovery (%) |
| 1 | 2.692 | 2.675 | 99.4 |
As can be seen from Table 5, the recovery rate of the sample is between 95% and 105%, which can meet the requirement of daily analysis precision, and it is shown that isobutanol can be used as an internal standard substance as an analysis method for determining ethanol under the condition of using DMF as a solvent.
Comparative example 1
And (3) detection of the separation degree:
preparing a DMF solution containing 0.16g/mL of n-propanol and 0.24g/mL of ethanol; a DMF solution containing 0.16g/mL isobutanol and 0.24g/mL ethanol was prepared.
According to the detection conditions of the embodiment, the influence of isobutanol as an internal standard substance and n-propanol as an internal standard substance in a medium with DMF as a solvent on the sample peak of ethanol is detected, and the specific result is shown in figure 1 with isobutanol as an internal standard substance, wherein in figure 1, the absorption peaks of an X axis from low to high are isobutanol, ethanol and DMF in sequence. According to verification, when the n-propanol is selected as the internal standard sample, the internal standard peak and the sample peak of the ethanol cannot be effectively separated, and when the isobutanol is selected, the sample peak of the ethanol and the internal standard peak are more beneficial to separation, so that the separation effect is better. Comparison of the degrees of separation in DMF medium with isobutanol as internal standard is shown in Table 6.
TABLE 6
| Internal standard substance | Degree of separation of ethanol from impurities |
| Isobutanol | 7.83 |
Detection example 3
Detection of long-term stability:
a sample solution which takes DMF as a solvent and isobutanol as an internal standard is applied to a brand new InertCap WAX chromatographic column for multiple sample injection, and the sample recovery rate of each sample injection is detected to verify the stability of the detection method. The results are shown in Table 7.
TABLE 7
The inventor of the invention finds that the recovery rate of a sample is 99.3% after 20 times of tests by using the solvent and the internal standard of the invention and adopting the same InertCap WAX chromatographic column, which indicates that the invention has better test stability.
The solvent and the internal standard of the invention have small influence on the polyethylene glycol stationary phase in the InertCap WAX chromatographic column, and can keep the stability of long-term detection.
In conclusion, the detection method disclosed by the invention is high in accuracy, high in detection stability and high in application value.
The preferred embodiments of the present invention have been described in detail, however, the present invention is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications are within the protective scope of the present invention.
It should be noted that the various technical features described in the above embodiments can be combined in any suitable manner without contradiction, and the invention is not described in any way for the possible combinations in order to avoid unnecessary repetition.
In addition, any combination of the various embodiments of the present invention is also possible, and the same should be considered as the disclosure of the present invention as long as it does not depart from the spirit of the present invention.
Claims (10)
1. A method for measuring ethanol content by gas chromatography in non-aqueous medium is characterized in that isobutanol is used as an internal standard substance, N-dimethylformamide is used as a solvent, and the detection conditions of the gas chromatography are as follows:
an InertCap WAX capillary column with the specification of 30m multiplied by 0.25mm and 0.25 mu m;
column temperature: the initial temperature is 100 ℃, the temperature is maintained for 5min, the temperature is increased to 240 ℃ at the speed of 120 ℃/min, and the temperature is maintained for 5 min;
the split ratio is as follows: 10: 1;
sample inlet temperature: 250 ℃;
detector temperature: 250 ℃, injection volume 0.5-1 μ L, column flow rate: 1.41mL/min, line speed: 35 cm/sec;
hydrogen gas: 30 mL/min;
air: 400mL/min, tail-blown: 12 mL/min.
2. The method of claim 1, comprising the steps of:
(1) preparing a standard solution by using ethanol and isobutanol as an internal standard substance as solutes and using N, N-dimethylformamide as a solvent;
(2) preparing a sample solution containing an internal standard substance;
(3) measuring peak areas of an internal standard substance and ethanol in the standard solution and the sample solution respectively by using a gas chromatograph, and calculating a mass correction factor f of the ethanol in the standard solution relative to the internal standard substance according to the following formulan：
fn＝Wn*AS/Ws*An
In the formula WsThe mass of the internal standard substance in the standard solution;
Wnthe mass of ethanol in the standard solution;
ASis the peak area of the internal standard in the standard solution;
Anis the peak area of ethanol in the standard solution;
(4) calculating the content of ethanol in the sample C%:
C％＝Ws*Ai*fn*100％/Wsp*Ais
wherein Wis is the mass of an internal standard substance in the sample solution;
Wspis the total mass of the sample;
Aiis the peak area of ethanol in the sample;
Aisis the peak area of the internal standard substance in the sample solution;
fnis the mass correction factor for ethanol in the sample.
3. The method according to claim 2, wherein the mass ratio of ethanol to the added internal standard in the standard solution is 0.3-3: 1.
4. The method according to claim 2, wherein the mass ratio of ethanol to the added internal standard in the standard solution is 0.5-2: 1.
5. The method according to claim 2, wherein the mass ratio of the total weight of the sample to the added internal standard is 0.3-3: 1.
6. The method according to claim 2, wherein the mass ratio of the total weight of the sample to the added internal standard is 0.5-2: 1.
7. The method according to any one of claims 2 to 6, wherein the concentration of ethanol in the standard solution is 0.16 to 0.24 g/mL.
8. The method according to any one of claims 2 to 6, wherein the concentration of ethanol in the standard solution is 0.16 to 0.24 g/mL.
9. The method according to any one of claims 1-6, wherein the stationary phase of the InertCap WAX capillary column is polyethylene glycol.
10. The method of any one of claims 1-6, wherein the InertCap WAX capillary column is DB-WAX or HP-WAX.
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| CN112517099A (en) * | 2020-12-25 | 2021-03-19 | 安徽精公检测检验中心有限公司 | Anti-suck-back device for ear washing ball |
| WO2023040391A1 (en) * | 2021-09-18 | 2023-03-23 | 天地壹号饮料股份有限公司 | Method for determining content of ethanol in wines on basis of gas chromatographic method |
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